AT3G22550

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009892
Physical Location & Seq
Reverse (-)
7991403 .. 7993588
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22550.1

Sequence Viewer

Length: 804 bp
ATGCTAAAGAAGAGATCAAGAAGCAAGCAAGCTTTAATGGCGGAAACAAACCAGAGTCAGAATCAGAAACAGAGTAAAACGACGCCGTTTCCGAGGCTCTTCACAGCTTTTAGTAGCTTTAAAAGCTTCACTGAAAACGACGCCGTCGCGAGTCCAACTTCAATTCTCGACACCAAACCTTTCTCTGTTTTGAAAAACCCATTTGGATCCGATAACCCGAAAACCCAAGAACCCGAAACCCGTCTCAAACTCGAACCCAAAAGAATCGGACTCGCCATTGTCGATTCTCTAATCCAAGACGAAACACCCGAACCCGGACCGAGATCCGGGACAATTCTATTCGGGTCTCAGCTTCGGATCCGGGTCCCGGATTCTCCGATTTCCTCGTCGGATTTCGGGATCAAAACGAGGAATTCTCAACCGGAGACGAAGAAACCGGGTTCCGAGTCGGGTCTTGGGTCTCCCAGAATCATCTCCGGCTATTTCCCGGCGAGCGATATGGAGTTATCGGAGGATTACACATGCGTGACGTGTCACGGGCCTAACCCGAGAACCATTCATATATTCGATAACTGTATCGTTGAGAGCCAACCCGGCGTCGTTTTCTTCCGGAGCTCCGACCCGGTTAATGAATCGGATTCGGATTATTCCCCACCCGACAGTTTTCTCAGTTGTTGCTGTAACTGTAAGAAGAGTCTTGGGCCTCGTGATGACATTTTCATGTACAGGGGAGATAGAGCTTTCTGTAGCAGTGAATGCAGGTCCATAGAGATGATGATGTCAGAGGAAAATGATACTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

267

Amino Acids

29.64

Weight (kDa)

5.85

Isoelectric Point (pI)

64.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 213 - 263 2.8e-20 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 750
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 609
AciI CCGC 1 cut(s) 41
AclWI GGATC 6 cut(s) 201, 214, 318, 352, 365, 407
AcsI RAATTY 1 cut(s) 412
AcyI GRCGYC 3 cut(s) 83, 141, 597
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 314, 326, 367
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 2 cut(s) 162, 193
AjiI CACGTC 1 cut(s) 531
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 7 cut(s) 32, 107, 117, 126, 352, 615, 740
AluI AGCT 7 cut(s) 32, 107, 117, 126, 352, 615, 740
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 4 cut(s) 248, 351, 419, 465
AlwI GGATC 6 cut(s) 201, 214, 318, 352, 365, 407
Ama87I CYCGRG 1 cut(s) 547
Aor13HI TCCGGA 1 cut(s) 609
AoxI GGCC 2 cut(s) 539, 701
ApoI RAATTY 1 cut(s) 412
AspS9I GGNCC 5 cut(s) 317, 364, 539, 701, 762
AsuC2I CCSGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 3 cut(s) 317, 364, 762
BamHI GGATCC 2 cut(s) 206, 357
BanII GRGCYC 1 cut(s) 617
BauI CACGAG 1 cut(s) 705
Bbv12I GWGCWC 1 cut(s) 617
BceAI ACGGC 2 cut(s) 70, 128
BcnI CCSGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
BcoDI GTCTC 4 cut(s) 248, 351, 419, 465
BfmI CTRYAG 1 cut(s) 745
BfuAI ACCTGC 1 cut(s) 750
BglI GCCNNNNNGGC 1 cut(s) 594
Bme1390I CCNGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
Bme18I GGWCC 3 cut(s) 317, 364, 762
BmeT110I CYCGRG 1 cut(s) 547
BmgBI CACGTC 1 cut(s) 531
BmgT120I GGNCC 5 cut(s) 317, 364, 539, 701, 762
BmiI GGNNCC 5 cut(s) 208, 359, 365, 366, 442
BmrFI CCNGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpuMI CCSGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
BsaHI GRCGYC 3 cut(s) 83, 141, 597
BsaI GGTCTC 2 cut(s) 351, 465
BsaJI CCNNGG 1 cut(s) 92
BsaWI WCCGGW 2 cut(s) 421, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 326, 367
BseAI TCCGGA 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 92
BseLI CCNNNNNNNGG 3 cut(s) 314, 326, 367
BseMII CTCAG 2 cut(s) 362, 682
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 2 cut(s) 541, 703
BsiHKAI GWGCWC 1 cut(s) 617
BsiHKCI CYCGRG 1 cut(s) 547
BslFI GGGAC 2 cut(s) 343, 350
BslI CCNNNNNNNGG 3 cut(s) 314, 326, 367
BsmAI GTCTC 4 cut(s) 248, 351, 419, 465
BsmBI CGTCTC 2 cut(s) 248, 419
BsmFI GGGAC 2 cut(s) 343, 350
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 2 cut(s) 541, 703
Bso31I GGTCTC 2 cut(s) 351, 465
BsoBI CYCGRG 1 cut(s) 547
Bsp1286I GDGCHC 1 cut(s) 617
Bsp13I TCCGGA 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 5 cut(s) 14, 206, 323, 357, 399
Bsp68I TCGCGA 1 cut(s) 149
BspACI CCGC 1 cut(s) 41
BspANI GGCC 2 cut(s) 541, 703
BspCNI CTCAG 2 cut(s) 361, 681
BspEI TCCGGA 1 cut(s) 609
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 5 cut(s) 208, 359, 365, 366, 442
BspMI ACCTGC 1 cut(s) 750
BspPI GGATC 6 cut(s) 201, 214, 318, 352, 365, 407
BspQI GCTCTTC 1 cut(s) 104
BspTNI GGTCTC 2 cut(s) 351, 465
BsrGI TGTACA 1 cut(s) 723
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 5 cut(s) 14, 206, 323, 357, 399
BssNI GRCGYC 3 cut(s) 83, 141, 597
BssSI CACGAG 1 cut(s) 705
Bst2BI CACGAG 1 cut(s) 705
Bst4CI ACNGT 3 cut(s) 575, 662, 686
Bst6I CTCTTC 3 cut(s) 5, 104, 686
BstACI GRCGYC 3 cut(s) 83, 141, 597
BstAPI GCANNNNNTGC 1 cut(s) 756
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 3 cut(s) 26, 30, 493
BstDEI CTNAG 2 cut(s) 348, 668
BstFNI CGCG 1 cut(s) 149
BstKTI GATC 5 cut(s) 17, 209, 326, 360, 402
BstMAI GTCTC 4 cut(s) 248, 351, 419, 465
BstMBI GATC 5 cut(s) 14, 206, 323, 357, 399
BstMWI GCNNNNNNNGC 4 cut(s) 38, 123, 594, 756
BstNSI RCATGY 1 cut(s) 525
BstSCI CCNGG 8 cut(s) 313, 326, 360, 366, 436, 486, 592, 621
BstSFI CTRYAG 1 cut(s) 745
BstUI CGCG 1 cut(s) 149
BstX2I RGATCY 3 cut(s) 206, 323, 357
BstYI RGATCY 3 cut(s) 206, 323, 357
BsuRI GGCC 2 cut(s) 541, 703
BtrI CACGTC 1 cut(s) 531
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 2 cut(s) 129, 757
BtuMI TCGCGA 1 cut(s) 149
BveI ACCTGC 1 cut(s) 750
Cac8I GCNNGC 3 cut(s) 26, 30, 493
Cfr13I GGNCC 5 cut(s) 317, 364, 539, 701, 762
CpoI CGGWCCG 1 cut(s) 317
CseI GACGC 3 cut(s) 91, 149, 586
Csp6I GTAC 1 cut(s) 724
CspI CGGWCCG 1 cut(s) 317
CviAII CATG 2 cut(s) 522, 721
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 2 cut(s) 348, 668
DpnI GATC 5 cut(s) 16, 208, 325, 359, 401
DpnII GATC 5 cut(s) 14, 206, 323, 357, 399
DraI TTTAAA 1 cut(s) 121
Eam1104I CTCTTC 3 cut(s) 5, 104, 686
EarI CTCTTC 3 cut(s) 5, 104, 686
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 615
Eco24I GRGCYC 1 cut(s) 617
Eco31I GGTCTC 2 cut(s) 351, 465
Eco47I GGWCC 3 cut(s) 317, 364, 762
Eco53kI GAGCTC 1 cut(s) 615
Eco88I CYCGRG 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 617
Esp3I CGTCTC 2 cut(s) 248, 419
FaeI CATG 2 cut(s) 525, 724
FaiI YATR 6 cut(s) 500, 523, 561, 563, 722, 767
FaqI GGGAC 2 cut(s) 343, 350
FatI CATG 2 cut(s) 521, 720
FriOI GRGCYC 1 cut(s) 617
HaeIII GGCC 2 cut(s) 541, 703
HgaI GACGC 3 cut(s) 91, 149, 586
Hin1I GRCGYC 3 cut(s) 83, 141, 597
Hin1II CATG 2 cut(s) 525, 724
HindIII AAGCTT 2 cut(s) 30, 124
Hpy188III TCNNGA 6 cut(s) 18, 148, 167, 397, 610, 707
Hpy99I CGWCG 5 cut(s) 85, 143, 149, 391, 602
HpyCH4III ACNGT 3 cut(s) 575, 662, 686
HpyCH4IV ACGT 1 cut(s) 530
HpyCH4V TGCA 1 cut(s) 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 123, 594, 756
HpyF3I CTNAG 2 cut(s) 348, 668
HpySE526I ACGT 1 cut(s) 530
Hsp92I GRCGYC 3 cut(s) 83, 141, 597
Hsp92II CATG 2 cut(s) 525, 724
KflI GGGWCCC 1 cut(s) 364
Kpn2I TCCGGA 1 cut(s) 609
Kzo9I GATC 5 cut(s) 14, 206, 323, 357, 399
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 2 cut(s) 612, 620
MaeII ACGT 1 cut(s) 530
MaeIII GTNAC 3 cut(s) 526, 533, 680
MalI GATC 5 cut(s) 16, 208, 325, 359, 401
MboI GATC 5 cut(s) 14, 206, 323, 357, 399
MboII GAAGA 5 cut(s) 22, 91, 442, 598, 703
MflI RGATCY 3 cut(s) 206, 323, 357
MhlI GDGCHC 1 cut(s) 617
MluCI AATT 3 cut(s) 162, 333, 412
MlyI GAGTC 5 cut(s) 64, 160, 264, 455, 703
MmeI TCCRAC 3 cut(s) 179, 369, 642
MnlI CCTC 6 cut(s) 87, 394, 402, 505, 714, 778
MroI TCCGGA 1 cut(s) 609
MseI TTAA 3 cut(s) 35, 120, 627
MslI CAYNNNNRTG 3 cut(s) 524, 719, 770
MspR9I CCNGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
Mva1269I GAATGC 1 cut(s) 761
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 38, 123, 594, 756
NciI CCSGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
NdeII GATC 5 cut(s) 14, 206, 323, 357, 399
NlaIII CATG 2 cut(s) 525, 724
NlaIV GGNNCC 5 cut(s) 208, 359, 365, 366, 442
NmuCI GTSAC 2 cut(s) 526, 533
NruI TCGCGA 1 cut(s) 149
NspI RCATGY 1 cut(s) 525
OliI CACNNNNGTG 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 3 cut(s) 89, 279, 306
PctI GAATGC 1 cut(s) 761
PfeI GAWTC 7 cut(s) 61, 264, 284, 371, 468, 632, 638
PflFI GACNNNGTC 1 cut(s) 143
PfoI TCCNGGA 2 cut(s) 326, 366
PleI GAGTC 5 cut(s) 63, 159, 264, 454, 702
PpsI GAGTC 5 cut(s) 63, 159, 264, 454, 702
PpuMI RGGWCCY 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 364
PspN4I GGNNCC 5 cut(s) 208, 359, 365, 366, 442
PspPI GGNCC 5 cut(s) 317, 364, 539, 701, 762
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 3 cut(s) 206, 323, 357
PsyI GACNNNGTC 1 cut(s) 143
RruI TCGCGA 1 cut(s) 149
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
RseI CAYNNNNRTG 3 cut(s) 524, 719, 770
Rsr2I CGGWCCG 1 cut(s) 317
RsrII CGGWCCG 1 cut(s) 317
SacI GAGCTC 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 35, 120, 627
Sau3AI GATC 5 cut(s) 14, 206, 323, 357, 399
Sau96I GGNCC 5 cut(s) 317, 364, 539, 701, 762
SchI GAGTC 5 cut(s) 64, 160, 264, 455, 703
ScrFI CCNGG 8 cut(s) 315, 328, 362, 368, 438, 488, 594, 623
SduI GDGCHC 1 cut(s) 617
SfcI CTRYAG 1 cut(s) 745
SinI GGWCC 3 cut(s) 317, 364, 762
SmiMI CAYNNNNRTG 3 cut(s) 524, 719, 770
Sse9I AATT 3 cut(s) 162, 333, 412
SsiI CCGC 1 cut(s) 41
SstI GAGCTC 1 cut(s) 617
StyD4I CCNGG 8 cut(s) 313, 326, 360, 366, 436, 486, 592, 621
TaaI ACNGT 3 cut(s) 575, 662, 686
TaiI ACGT 1 cut(s) 533
TaqI TCGA 4 cut(s) 168, 252, 282, 567
TaqII GACCGA 1 cut(s) 334
TasI AATT 3 cut(s) 162, 333, 412
TatI WGTACW 1 cut(s) 723
TfiI GAWTC 7 cut(s) 61, 264, 284, 371, 468, 632, 638
Tru1I TTAA 3 cut(s) 35, 120, 627
Tru9I TTAA 3 cut(s) 35, 120, 627
TscAI CASTG 2 cut(s) 136, 757
TseFI GTSAC 2 cut(s) 526, 533
Tsp45I GTSAC 2 cut(s) 526, 533
TspDTI ATGAA 3 cut(s) 548, 645, 709
TspRI CASTG 2 cut(s) 136, 757
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 3 cut(s) 317, 364, 762
XapI RAATTY 1 cut(s) 412
XceI RCATGY 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.