pycom13g16750

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009892
Physical Location & Seq
Reverse (-)
12506851 .. 12508702
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16750.1

Sequence Viewer

Length: 873 bp
ATGGCGGAAAACGGCTCTCTTCTCTCTCCCAGAGAAAAACACACAAAACTCACTTCCTCTTTCTTCGCGAATCCGAGATTGTTCACCAGCTTTACCTCAAAAGCCGTCTCTGAATCCGACGCCGTAATGAGCCCAACCTCCATCCTCGAAACGAAACCGTTTCTGGGCCTCCGAAACCCCTTCTGGTCCGAATCGAACACCCCCAGAACCCCGGAACCCGAATCCAGACGCATCCACTGGGACAAATTGGACTGCAAAGGCATCGGCCTCGCCATCGTCGACGCCCTCAACGAATCCGACCCGAAACCGTCAAATCCCAAAACCCGAATGGTGATTTTCGGGTCGCAGCTCAAGATCCAAATACCTCCCCTGAAACCCTCTGTTCTCTCCCCTTCCCGATCAACGAAATCTTCGGCGGATTTCAGCACCAAGACGGAGAATTCCCATCTGGGTTCTTTCTCCTCCGTCTTGTCTCAGTCGCCGGAGAAGAAATCGCTGTTCGAATCCGCGTACTCCGGGCTCGAGACGAAGAATTCGACCCGGGTTTTCACGAGCTGTCTCTCTGTCAGCGAAATGGAGCTCTCTGAGGATTACACGTGTGTGATTTCCCATAGGCCGAACCCGAAAACCACGCACATCTTCGACAATTGCATCGTGGATAGCTCCGATGGCGTCCCGGAGTTTTCTCCCGGCAGGAAGTCAAACGGGTCAAGCTCTCTGTCTGAGAGTTTTCTCAGCTTCTGTGACAATTGCAAGACGAATCTTGGCCAGGGCAAAGACATTTTTATGTACAGGGGTGAGAAGGCATTCTGCAGCCGTGAGTGCCGTTACCAGGAGATGTTGAAGGAGGGAGTGGATAAACTGGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

291

Amino Acids

32.1

Weight (kDa)

7.55

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 237 - 282 5.1e-20 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 279
AccII CGCG 2 cut(s) 68, 509
AciI CCGC 3 cut(s) 5, 416, 507
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 766
AcsI RAATTY 2 cut(s) 439, 532
AcvI CACGTG 1 cut(s) 597
AcyI GRCGYC 3 cut(s) 120, 282, 672
AfaI GTAC 2 cut(s) 512, 791
AfiI CCNNNNNNNGG 2 cut(s) 164, 832
AflIII ACRYGT 2 cut(s) 594, 596
AgsI TTSAA 1 cut(s) 844
AjnI CCWGG 2 cut(s) 768, 831
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 8 cut(s) 90, 349, 555, 580, 663, 714, 738, 869
AluI AGCT 8 cut(s) 90, 349, 555, 580, 663, 714, 738, 869
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 4 cut(s) 112, 477, 518, 563
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 741
Ama87I CYCGRG 2 cut(s) 521, 540
AoxI GGCC 4 cut(s) 166, 265, 614, 766
ApeKI GCWGC 2 cut(s) 346, 813
ApoI RAATTY 2 cut(s) 439, 532
ArsI GACNNNNNNTTYG 2 cut(s) 517, 549
Asp700I GAANNNNTTC 1 cut(s) 806
AspS9I GGNCC 2 cut(s) 166, 186
AsuC2I CCSGG 6 cut(s) 212, 517, 541, 542, 677, 690
AsuHPI GGTGA 3 cut(s) 76, 343, 809
AsuII TTCGAA 1 cut(s) 501
AvaI CYCGRG 2 cut(s) 521, 540
AvaII GGWCC 1 cut(s) 186
BalI TGGCCA 1 cut(s) 768
BanII GRGCYC 3 cut(s) 134, 522, 582
BauI CACGAG 1 cut(s) 550
BbrPI CACGTG 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 2 cut(s) 358, 825
BccI CCATC 4 cut(s) 149, 281, 453, 662
BceAI ACGGC 5 cut(s) 28, 89, 107, 801, 810
BcgI CGANNNNNNTGC 4 cut(s) 244, 250, 278, 284
BciT130I CCWGG 2 cut(s) 770, 833
BcnI CCSGG 6 cut(s) 212, 517, 541, 542, 677, 690
BcoDI GTCTC 4 cut(s) 112, 477, 518, 563
BfmI CTRYAG 1 cut(s) 811
BisI GCNGC 2 cut(s) 347, 814
BlsI GCNGC 2 cut(s) 348, 815
Bme1390I CCNGG 8 cut(s) 212, 517, 541, 542, 677, 690, 770, 833
Bme18I GGWCC 1 cut(s) 186
BmeT110I CYCGRG 2 cut(s) 521, 540
BmgT120I GGNCC 2 cut(s) 166, 186
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 8 cut(s) 212, 517, 541, 542, 677, 690, 770, 833
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 3 cut(s) 240, 270, 660
BmuI ACTGGG 1 cut(s) 247
Bpu14I TTCGAA 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 6 cut(s) 212, 517, 541, 542, 677, 690
BsaAI YACGTR 1 cut(s) 597
BsaHI GRCGYC 3 cut(s) 120, 282, 672
BsaJI CCNNGG 3 cut(s) 210, 540, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 832
Bse1I ACTGG 2 cut(s) 242, 867
BseBI CCWGG 2 cut(s) 770, 833
BseDI CCNNGG 3 cut(s) 210, 540, 769
BseGI GGATG 2 cut(s) 141, 231
BseLI CCNNNNNNNGG 2 cut(s) 164, 832
BseMII CTCAG 4 cut(s) 488, 576, 714, 748
BseNI ACTGG 2 cut(s) 242, 867
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 2 cut(s) 358, 825
Bsh1236I CGCG 2 cut(s) 68, 509
BshFI GGCC 4 cut(s) 168, 267, 616, 768
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 2 cut(s) 521, 540
BsiSI CCGG 6 cut(s) 212, 482, 516, 541, 677, 690
BslFI GGGAC 2 cut(s) 254, 659
BslI CCNNNNNNNGG 2 cut(s) 164, 832
BsmAI GTCTC 4 cut(s) 112, 477, 518, 563
BsmBI CGTCTC 2 cut(s) 112, 518
BsmFI GGGAC 2 cut(s) 254, 659
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 4 cut(s) 168, 267, 616, 768
BsoBI CYCGRG 2 cut(s) 521, 540
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 3 cut(s) 134, 522, 582
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 2 cut(s) 354, 398
Bsp68I TCGCGA 1 cut(s) 68
BspACI CCGC 3 cut(s) 5, 416, 507
BspANI GGCC 4 cut(s) 168, 267, 616, 768
BspCNI CTCAG 4 cut(s) 487, 577, 715, 747
BspFNI CGCG 2 cut(s) 68, 509
BspLI GGNNCC 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 815
BspPI GGATC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 501
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 2 cut(s) 242, 867
BssECI CCNNGG 3 cut(s) 210, 540, 769
BssMI GATC 2 cut(s) 354, 398
BssNI GRCGYC 3 cut(s) 120, 282, 672
BssSI CACGAG 1 cut(s) 550
Bst2BI CACGAG 1 cut(s) 550
Bst2UI CCWGG 2 cut(s) 770, 833
Bst4CI ACNGT 2 cut(s) 159, 309
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 3 cut(s) 120, 282, 672
BstAUI TGTACA 1 cut(s) 789
BstBAI YACGTR 1 cut(s) 597
BstBI TTCGAA 1 cut(s) 501
BstDEI CTNAG 4 cut(s) 474, 585, 723, 734
BstF5I GGATG 2 cut(s) 141, 231
BstFNI CGCG 2 cut(s) 68, 509
BstKTI GATC 2 cut(s) 357, 401
BstMAI GTCTC 4 cut(s) 112, 477, 518, 563
BstMBI GATC 2 cut(s) 354, 398
BstMWI GCNNNNNNNGC 2 cut(s) 669, 822
BstNI CCWGG 2 cut(s) 770, 833
BstSCI CCNGG 8 cut(s) 210, 515, 539, 540, 675, 688, 768, 831
BstSFI CTRYAG 1 cut(s) 811
BstUI CGCG 2 cut(s) 68, 509
BstV1I GCAGC 2 cut(s) 358, 825
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 4 cut(s) 168, 267, 616, 768
BtsCI GGATG 2 cut(s) 141, 231
BtsIMutI CAGTG 1 cut(s) 235
BtuMI TCGCGA 1 cut(s) 68
CaiI CAGNNNCTG 1 cut(s) 741
Cfr13I GGNCC 2 cut(s) 166, 186
Cfr9I CCCGGG 1 cut(s) 540
CseI GACGC 4 cut(s) 128, 237, 290, 661
Csp6I GTAC 2 cut(s) 511, 790
CviQI GTAC 2 cut(s) 511, 790
DdeI CTNAG 4 cut(s) 474, 585, 723, 734
DpnI GATC 2 cut(s) 356, 400
DpnII GATC 2 cut(s) 354, 398
EaeI YGGCCR 1 cut(s) 766
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 2 cut(s) 20, 431
Ecl136II GAGCTC 1 cut(s) 580
Eco24I GRGCYC 3 cut(s) 134, 522, 582
Eco47I GGWCC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 580
Eco72I CACGTG 1 cut(s) 597
Eco88I CYCGRG 2 cut(s) 521, 540
EcoICRI GAGCTC 1 cut(s) 580
EcoRI GAATTC 2 cut(s) 439, 532
EcoRII CCWGG 2 cut(s) 768, 831
EcoT38I GRGCYC 3 cut(s) 134, 522, 582
Esp3I CGTCTC 2 cut(s) 112, 518
FaiI YATR 2 cut(s) 612, 788
FaqI GGGAC 2 cut(s) 254, 659
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 347, 814
FokI GGATG 2 cut(s) 128, 218
FriOI GRGCYC 3 cut(s) 134, 522, 582
Fsp4HI GCNGC 2 cut(s) 347, 814
GluI GCNGC 2 cut(s) 347, 814
HaeIII GGCC 4 cut(s) 168, 267, 616, 768
HapII CCGG 6 cut(s) 212, 482, 516, 541, 677, 690
HgaI GACGC 4 cut(s) 128, 237, 290, 661
Hin1I GRCGYC 3 cut(s) 120, 282, 672
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 867
HinfI GANTC 7 cut(s) 70, 113, 191, 221, 293, 503, 760
HpaII CCGG 6 cut(s) 212, 482, 516, 541, 677, 690
HphI GGTGA 3 cut(s) 76, 343, 809
Hpy166II GTNNAC 2 cut(s) 84, 280
Hpy188I TCNGA 9 cut(s) 75, 112, 118, 173, 190, 298, 586, 667, 724
Hpy188III TCNNGA 6 cut(s) 67, 225, 352, 396, 523, 550
Hpy8I GTNNAC 2 cut(s) 84, 280
Hpy99I CGWCG 3 cut(s) 122, 281, 284
HpyAV CCTTC 4 cut(s) 190, 402, 796, 838
HpyCH4III ACNGT 2 cut(s) 159, 309
HpyCH4IV ACGT 1 cut(s) 596
HpyCH4V TGCA 4 cut(s) 255, 651, 753, 813
HpyF10VI GCNNNNNNNGC 2 cut(s) 669, 822
HpyF3I CTNAG 4 cut(s) 474, 585, 723, 734
HpySE526I ACGT 1 cut(s) 596
Hsp92I GRCGYC 3 cut(s) 120, 282, 672
Kzo9I GATC 2 cut(s) 354, 398
LmnI GCTCC 2 cut(s) 577, 668
Lsp1109I GCAGC 2 cut(s) 358, 825
LweI GCATC 3 cut(s) 240, 270, 660
MaeII ACGT 1 cut(s) 596
MaeIII GTNAC 2 cut(s) 743, 827
MalI GATC 2 cut(s) 356, 400
MboI GATC 2 cut(s) 354, 398
MboII GAAGA 6 cut(s) 11, 55, 402, 499, 541, 631
MfeI CAATTG 2 cut(s) 646, 748
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 3 cut(s) 134, 522, 582
MlsI TGGCCA 1 cut(s) 768
MluCI AATT 5 cut(s) 245, 439, 532, 646, 748
MluNI TGGCCA 1 cut(s) 768
MmeI TCCRAC 2 cut(s) 141, 321
Mox20I TGGCCA 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 806
MscI TGGCCA 1 cut(s) 768
MslI CAYNNNNRTG 2 cut(s) 599, 785
Msp20I TGGCCA 1 cut(s) 768
MspI CCGG 6 cut(s) 212, 482, 516, 541, 677, 690
MspR9I CCNGG 8 cut(s) 212, 517, 541, 542, 677, 690, 770, 833
MunI CAATTG 2 cut(s) 646, 748
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 2 cut(s) 770, 833
MvnI CGCG 2 cut(s) 68, 509
MwoI GCNNNNNNNGC 2 cut(s) 669, 822
NciI CCSGG 6 cut(s) 212, 517, 541, 542, 677, 690
NdeII GATC 2 cut(s) 354, 398
NlaIV GGNNCC 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 743
NruI TCGCGA 1 cut(s) 68
NspV TTCGAA 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 521
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 288, 533
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 7 cut(s) 70, 113, 191, 221, 293, 503, 760
PfoI TCCNGGA 1 cut(s) 675
PkrI GCNGC 2 cut(s) 348, 815
PmaCI CACGTG 1 cut(s) 597
PmlI CACGTG 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 597
Psp124BI GAGCTC 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 768, 831
PspCI CACGTG 1 cut(s) 597
PspGI CCWGG 2 cut(s) 768, 831
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 166, 186
PstI CTGCAG 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 741
PsuI RGATCY 1 cut(s) 354
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 2 cut(s) 512, 791
RsaNI GTAC 2 cut(s) 511, 790
RseI CAYNNNNRTG 2 cut(s) 599, 785
SacI GAGCTC 1 cut(s) 582
SalI GTCGAC 1 cut(s) 278
SatI GCNGC 2 cut(s) 347, 814
Sau3AI GATC 2 cut(s) 354, 398
Sau96I GGNCC 2 cut(s) 166, 186
ScrFI CCNGG 8 cut(s) 212, 517, 541, 542, 677, 690, 770, 833
SduI GDGCHC 3 cut(s) 134, 522, 582
SfaNI GCATC 3 cut(s) 240, 270, 660
SfcI CTRYAG 1 cut(s) 811
Sfr274I CTCGAG 1 cut(s) 521
SfuI TTCGAA 1 cut(s) 501
SgrDI CGTCGACG 1 cut(s) 278
SinI GGWCC 1 cut(s) 186
SlaI CTCGAG 1 cut(s) 521
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 599, 785
SmlI CTYRAG 2 cut(s) 350, 521
SmoI CTYRAG 2 cut(s) 350, 521
Sse9I AATT 5 cut(s) 245, 439, 532, 646, 748
SsiI CCGC 3 cut(s) 5, 416, 507
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 8 cut(s) 210, 515, 539, 540, 675, 688, 768, 831
TaaI ACNGT 2 cut(s) 159, 309
TaiI ACGT 1 cut(s) 599
TaqI TCGA 7 cut(s) 147, 194, 279, 501, 522, 536, 642
TasI AATT 5 cut(s) 245, 439, 532, 646, 748
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 7 cut(s) 70, 113, 191, 221, 293, 503, 760
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 346, 813
Tsp45I GTSAC 1 cut(s) 743
TspGWI ACGGA 2 cut(s) 449, 454
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 2 cut(s) 439, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 521
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.