AT3G23640

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005660
Physical Location & Seq
Forward (+)
8501556 .. 8509649
8094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23640.2

Sequence Viewer

Length: 2976 bp
ATGACTTTAAGTGGGGATAGCTCGGAAACTGTAGAGATGACTTCAACTGATATGATTTTCGAGCCGATTTTGGAGCATGGAGTTTTCCGGTTTGATTGTTCTGTTGACCATAGGAAGGCTGCTTTTCCTAGTGTTTCATTTAAGAACAGCAAAGACCGGGAAGTTCCTATTGTGAGTCACATTGTTCCAGCATATATTCCTACTTGTGGTTGTCTTCAGGACCAACAAGTTGTCACCTTTGAGTTTTCTCCTGGTACATCGTTTTATGGGACGGGAGAAGTTAGTGGTCAGTTGGAGAGAACAGGCAAACGGGTTTTTACTTGGAACACTGATGCATGGGGATATGGTTCTGGAACCACTTCGTTATACCAGTCACATCCTTGGGTACTAGTTGTCCTTCCAACTGGAGAAACACTAGGGGTTCTTGCTGATACAACACGAAAGTGTGAGATTGATCTACGGAAAGAGGGAATCATAAGAATTATCTCCCCAGCATCATATCCAATAATCACATTTGGTCCATTCTCCTCGCCTACTGCTGTCTTGGAGTCCCTCTCGCACGCCATTGGAACTGTGTTTATGCCTCCGAAGTGGGCCTTAGGCTACCACCAATGCCGTTGGAGTTATATGTCTGACAAGCGAGTAGCCGAGATCGCTCAAACATTTCGCGACAAGAAAATTCCTTCTGACGTGATATGGATGGATATTGACTACATGGATGGTTTCCGTTGTTTCACATTCGACAAGGAGCGTTTTCCGGATCCAAGTGCTTTGGCCAAAGATCTCCATAGTAATGGTTTCAAAGCTATCTGGATGCTTGACCCTGGAATAAAGCAGGAGGAAGGTTATTATGTCTACGATAGTGGGAGTAAAAATGATGTTTGGATTAGCCGAGCAGATGGGAAGCCATTTACTGGTGAAGTATGGCCTGGGCCTTGCGTTTTCCCTGACTATACAAATTCCAAAGCACGCTCTTGGTGGGCTAATTTGGTTAAGGAATTTGTTTCAAATGGTGTTGATGGTATATGGAATGATATGAATGAGCCAGCTGTTTTCAAAGTCGTGACAAAGACGATGCCTGAGAACAATATTCACCATGGAGATGATGAGCTTGGAGGTGTTCAAAACCACTCACACTACCACAATGTCTATGGGATGCTGATGGCAAGATCAACTTACGAAGGGATGGAGCTAGCTGATAAGAATAAGCGACCTTTTGTATTGACAAGGGCTGGATTCATAGGTAGCCAGAGATATGCTGCGACTTGGACAGGAGATAACCTTTCAAACTGGGAGCATCTACATATGTCTATATCCATGGTCCTTCAGCTGGGGCTTAGTGGTCAGCCCTTATCAGGGCCAGATATCGGTGGCTTTGCTGGCAATGCAACTCCGCGGCTATTTGGACGTTGGATGGGTGTTGGAGCTATGTTTCCATTCTGTCGTGGCCATTCCGAGGCTGGCACTGATGACCATGAACCATGGTCCTTTGGAGAAGAGTGTGAGGAAGTCTGTCGTGCCGCATTGAAGAGGCGTTACCAACTCCTACCACATTTTTACACCTTGTTTTACATAGCCCATACAACTGGTGCTCCAGTTGCAGCTCCAATCTTTTTCGCTGATCCAATAGATTCCCGGCTAAGAGCTGTTGAAAATGGCTTTCTTTTGGGCCCACTTCTTATATATGCAAGCACTTTGTCTAGTCAGGGATCACATGAGCTGCAGCACATATTACCGAGAGGAATTTGGCATAGGTTTGATTTTGCAGATTCACATCCGGATTTACCAACTTTATATTTACAAGGGGGATCCATCATATCATTGGCTCCTCCACATCTGCATGTTGGGGAGTTTAGTCTGTCAGATGACTTGACCCTACTTGTGTCATTAGATGAAAATGGGAAAGCTAAAGGACTCTTGTTCGAAGATGATGGAGATGGATATGGCTACACTAAAGGAAGATTTCTAGTTACACACTACATTGCTGAGCGGGATTCTTCCACTGTCACCGTCAAGGTTTCAAAAACTGAAGGAGACTGGCAGAGGCCAAACCGCCGTGTTCATGTCCAATTATTACTAGGAGGCGGTGCAATGCTTGATGCTTGGGGAATGGATGGAGAGTTTATCCACATTAAGGTGCCTTCAGAAAGTGGAATTTCAGAGTTAATATCCACAAGCAATGAGCGCTTTAAACTTCATATGGAAAATACAAAACTGATACCTGAGAAGGAAGTGGTACCTGGACAAAAGGGAATGGAACTTTCAAAGGAGCCAGTTGAGTTAAGCAGTGGTGATTGGAAACTTAATATAGTTCCCTGGGTTGGTGGAAGGATATTGTCTATGACACATGTTCCCTCAGGAATTCAATGGCTTCATAGCAGGATAGATATCAATGGTTATGAGGAGTATAGTGGTACTGAGTACCGGTCAGCTGGATGTACTGAGGAATACAATGTCATTGAGAGGGATTTGGAGCATGCAGGAGAAGAAGAATCTCTGATCCTAGAAGGTGATGTAGGTGGTGGACTGGTTCTTCGGCGTAAAATAAGTATAGCTAAAGACAATCAACGAGTTTTCCGGATTGCCTCTAGCATTGAAGCCCGTAGTGTTGGTGCTGGTTCTGGTGGATTTTCAAGGCTGGTATGCTTAAGAGTCCATCCAACTTTCACTCTTTTGCATCCGACTGAATCGTTTGTCTCTTTCACGTCGATTGATGGTTCAAAGCATGAAGTTTGGCCAGATTCTGGAGACCAAATATATGAAGGGAACAACCTTCCACATGGGAAATGGATGTTGGTGGACAAGAGCCTCAATCTTCGGATGGTAAACAGGTTCGATGTTAGTCAGGTCTTCAAATGTATTATCCACTGGGACTGTGGAACTGTTAACTTAGAGCTATGGTCTAAAGAGCGGCCTGTTTCCAAGGAATCTCCTCTGAAAATAGAGCATGAATATGAAGTCACAAGTTTCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

991

Amino Acids

110.88

Weight (kDa)

5.57

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 78 - 152 2.7e-17 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 193 - 522 1.3e-126 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 624 - 692 3.4e-13 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2236
AccB1I GGYRCC 2 cut(s) 2137, 2236
AccB7I CCANNNNNTGG 2 cut(s) 914, 2744
AccBSI CCGCTC 2 cut(s) 1990, 2911
AccI GTMKAC 1 cut(s) 855
AccII CGCG 2 cut(s) 669, 1396
AccIII TCCGGA 3 cut(s) 757, 1777, 2577
AciI CCGC 7 cut(s) 1394, 1396, 1521, 1990, 2053, 2085, 2911
AclWI GGATC 7 cut(s) 755, 768, 1616, 1717, 1803, 1816, 2494
AcoI YGGCCR 3 cut(s) 774, 1447, 2735
AcsI RAATTY 6 cut(s) 678, 958, 998, 1743, 2154, 2361
AcuI CTGAAG 4 cut(s) 200, 1310, 2049, 2127
AfaI GTAC 6 cut(s) 256, 387, 2238, 2416, 2423, 2440
AfeI AGCGCT 1 cut(s) 2186
AflII CTTAAG 1 cut(s) 2647
AflIII ACRYGT 1 cut(s) 2347
AgeI ACCGGT 1 cut(s) 2424
AhlI ACTAGT 1 cut(s) 388
AjiI CACGTC 2 cut(s) 691, 2706
AjnI CCWGG 5 cut(s) 250, 823, 928, 2239, 2315
AjuI GAANNNNNNNTTGG 2 cut(s) 2777, 2809
AleI CACNNNNGTG 1 cut(s) 442
Alw21I GWGCWC 1 cut(s) 1594
Alw26I GTCTC 3 cut(s) 2028, 2701, 2742
AlwI GGATC 7 cut(s) 755, 768, 1616, 1717, 1803, 1816, 2494
AlwNI CAGNNNCTG 1 cut(s) 2744
Aor13HI TCCGGA 3 cut(s) 757, 1777, 2577
Aor51HI AGCGCT 1 cut(s) 2186
ApaI GGGCCC 1 cut(s) 1673
ApeKI GCWGC 5 cut(s) 119, 1259, 1601, 1720, 1723
ApoI RAATTY 6 cut(s) 678, 958, 998, 1743, 2154, 2361
ArsI GACNNNNNNTTYG 2 cut(s) 2517, 2549
AsiGI ACCGGT 1 cut(s) 2424
Asp700I GAANNNNTTC 1 cut(s) 358
Asp718I GGTACC 1 cut(s) 2236
AspLEI GCGC 1 cut(s) 2187
AspS9I GGNCC 9 cut(s) 220, 518, 594, 932, 1321, 1358, 1485, 1669, 1670
AsuC2I CCSGG 2 cut(s) 158, 1636
AsuHPI GGTGA 6 cut(s) 226, 929, 1085, 1999, 2303, 2522
AsuII TTCGAA 1 cut(s) 1923
AsuNHI GCTAGC 1 cut(s) 1192
AvaII GGWCC 4 cut(s) 220, 518, 1321, 1485
AxyI CCTNAGG 2 cut(s) 598, 2356
BaeGI GKGCMC 1 cut(s) 1673
BalI TGGCCA 3 cut(s) 776, 1449, 2737
BamHI GGATCC 2 cut(s) 760, 1808
BanI GGYRCC 2 cut(s) 2137, 2236
BanII GRGCYC 1 cut(s) 1673
BarI GAAGNNNNNNTAC 2 cut(s) 1520, 1552
BbsI GAAGAC 2 cut(s) 206, 2842
Bbv12I GWGCWC 1 cut(s) 1594
BbvI GCAGC 5 cut(s) 106, 1246, 1613, 1707, 1735
BceAI ACGGC 2 cut(s) 600, 2040
BciT130I CCWGG 5 cut(s) 252, 825, 930, 2241, 2317
BcnI CCSGG 2 cut(s) 158, 1636
BcoDI GTCTC 3 cut(s) 2028, 2701, 2742
BcuI ACTAGT 1 cut(s) 388
BfaI CTAG 9 cut(s) 129, 389, 416, 1193, 1701, 1967, 2078, 2504, 2589
BfmI CTRYAG 2 cut(s) 30, 1721
BfoI RGCGCY 1 cut(s) 2188
BfrI CTTAAG 1 cut(s) 2647
BglII AGATCT 1 cut(s) 781
BisI GCNGC 8 cut(s) 120, 1260, 1397, 1521, 1602, 1721, 1724, 2912
BlpI GCTNAGC 1 cut(s) 1986
BlsI GCNGC 8 cut(s) 121, 1261, 1398, 1522, 1603, 1722, 1725, 2913
Bme1390I CCNGG 7 cut(s) 158, 252, 825, 930, 1636, 2241, 2317
Bme18I GGWCC 4 cut(s) 220, 518, 1321, 1485
BmgBI CACGTC 2 cut(s) 691, 2706
BmgT120I GGNCC 9 cut(s) 220, 518, 594, 932, 1321, 1358, 1485, 1669, 1670
BmiI GGNNCC 8 cut(s) 355, 762, 1671, 1810, 1827, 2139, 2238, 2271
BmrFI CCNGG 7 cut(s) 158, 252, 825, 930, 1636, 2241, 2317
BmrI ACTGGG 2 cut(s) 1300, 2878
BmsI GCATC 8 cut(s) 322, 503, 804, 1065, 1146, 1306, 2089, 2686
BmtI GCTAGC 1 cut(s) 1196
BmuI ACTGGG 2 cut(s) 1300, 2878
BpiI GAAGAC 2 cut(s) 206, 2842
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpmI CTGGAG 3 cut(s) 426, 1578, 2766
Bpu1102I GCTNAGC 1 cut(s) 1986
Bpu14I TTCGAA 1 cut(s) 1923
BpuMI CCSGG 2 cut(s) 158, 1636
BsaBI GATNNNNATC 2 cut(s) 1773, 2387
BsaI GGTCTC 1 cut(s) 2742
BsaWI WCCGGW 5 cut(s) 87, 757, 1777, 2424, 2577
BsaXI ACNNNNNCTCC 2 cut(s) 1576, 1606
Bse118I RCCGGY 1 cut(s) 2424
Bse21I CCTNAGG 2 cut(s) 598, 2356
Bse3DI GCAATG 4 cut(s) 1390, 1980, 2097, 2185
Bse8I GATNNNNATC 2 cut(s) 1773, 2387
BseAI TCCGGA 3 cut(s) 757, 1777, 2577
BseBI CCWGG 5 cut(s) 252, 825, 930, 2241, 2317
BseJI GATNNNNATC 2 cut(s) 1773, 2387
BseMI GCAATG 4 cut(s) 1390, 1980, 2097, 2185
BseMII CTCAG 6 cut(s) 1071, 1977, 2214, 2370, 2409, 2433
BseRI GAGGAG 4 cut(s) 517, 1818, 2417, 2922
BseSI GKGCMC 1 cut(s) 1673
BseXI GCAGC 5 cut(s) 106, 1246, 1613, 1707, 1735
BseYI CCCAGC 2 cut(s) 490, 1330
Bsh1236I CGCG 2 cut(s) 669, 1396
BshNI GGYRCC 2 cut(s) 2137, 2236
BshTI ACCGGT 1 cut(s) 2424
BsiHKAI GWGCWC 1 cut(s) 1594
BsiSI CCGG 7 cut(s) 88, 157, 758, 1636, 1778, 2425, 2578
BslFI GGGAC 3 cut(s) 283, 535, 2885
BsmAI GTCTC 3 cut(s) 2028, 2701, 2742
BsmFI GGGAC 3 cut(s) 283, 535, 2885
Bso31I GGTCTC 1 cut(s) 2742
Bsp119I TTCGAA 1 cut(s) 1923
Bsp120I GGGCCC 1 cut(s) 1669
Bsp1286I GDGCHC 2 cut(s) 1594, 1673
Bsp13I TCCGGA 3 cut(s) 757, 1777, 2577
Bsp143I GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
Bsp1720I GCTNAGC 1 cut(s) 1986
Bsp19I CCATGG 3 cut(s) 1096, 1317, 1481
Bsp68I TCGCGA 1 cut(s) 669
BspACI CCGC 7 cut(s) 1394, 1396, 1521, 1990, 2053, 2085, 2911
BspCNI CTCAG 6 cut(s) 1072, 1978, 2215, 2369, 2410, 2434
BspEI TCCGGA 3 cut(s) 757, 1777, 2577
BspFNI CGCG 2 cut(s) 669, 1396
BspLI GGNNCC 8 cut(s) 355, 762, 1671, 1810, 1827, 2139, 2238, 2271
BspMAI CTGCAG 1 cut(s) 1725
BspOI GCTAGC 1 cut(s) 1196
BspPI GGATC 7 cut(s) 755, 768, 1616, 1717, 1803, 1816, 2494
BspT104I TTCGAA 1 cut(s) 1923
BspT107I GGYRCC 2 cut(s) 2137, 2236
BspTI CTTAAG 1 cut(s) 2647
BspTNI GGTCTC 1 cut(s) 2742
BsrBI CCGCTC 2 cut(s) 1990, 2911
BsrDI GCAATG 4 cut(s) 1390, 1980, 2097, 2185
BsrFI RCCGGY 1 cut(s) 2424
BssAI RCCGGY 1 cut(s) 2424
BssMI GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
BssT1I CCWWGG 5 cut(s) 380, 1096, 1317, 1481, 2922
Bst2UI CCWGG 5 cut(s) 252, 825, 930, 2241, 2317
Bst4CI ACNGT 6 cut(s) 31, 574, 2005, 2011, 2876, 2884
Bst6I CTCTTC 2 cut(s) 1491, 1523
BstAFI CTTAAG 1 cut(s) 2647
BstBI TTCGAA 1 cut(s) 1923
BstC8I GCNNGC 8 cut(s) 561, 970, 1047, 1194, 1381, 1462, 1690, 2478
BstDSI CCRYGG 4 cut(s) 1096, 1317, 1394, 1481
BstFNI CGCG 2 cut(s) 669, 1396
BstH2I RGCGCY 1 cut(s) 2188
BstHHI GCGC 1 cut(s) 2187
BstKTI GATC 9 cut(s) 457, 654, 763, 784, 1172, 1624, 1712, 1811, 2502
BstMAI GTCTC 3 cut(s) 2028, 2701, 2742
BstMBI GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
BstMWI GCNNNNNNNGC 5 cut(s) 653, 1380, 1385, 1598, 2184
BstNI CCWGG 5 cut(s) 252, 825, 930, 2241, 2317
BstNSI RCATGY 3 cut(s) 1844, 2351, 2480
BstSCI CCNGG 7 cut(s) 156, 250, 823, 928, 1634, 2239, 2315
BstSFI CTRYAG 2 cut(s) 30, 1721
BstSLI GKGCMC 1 cut(s) 1673
BstUI CGCG 2 cut(s) 669, 1396
BstV1I GCAGC 5 cut(s) 106, 1246, 1613, 1707, 1735
BstV2I GAAGAC 2 cut(s) 206, 2842
BstX2I RGATCY 3 cut(s) 760, 781, 1808
BstXI CCANNNNNNTGG 2 cut(s) 794, 1586
BstYI RGATCY 3 cut(s) 760, 781, 1808
Bsu36I CCTNAGG 2 cut(s) 598, 2356
BtgI CCRYGG 4 cut(s) 1096, 1317, 1394, 1481
BtrI CACGTC 2 cut(s) 691, 2706
BtsI GCAGTG 1 cut(s) 2293
BtsIMutI CAGTG 5 cut(s) 327, 1464, 2001, 2293, 2866
BtuMI TCGCGA 1 cut(s) 669
Cac8I GCNNGC 8 cut(s) 561, 970, 1047, 1194, 1381, 1462, 1690, 2478
CaiI CAGNNNCTG 1 cut(s) 2744
CfoI GCGC 1 cut(s) 2187
Cfr10I RCCGGY 1 cut(s) 2424
Cfr13I GGNCC 9 cut(s) 220, 518, 594, 932, 1321, 1358, 1485, 1669, 1670
Cfr42I CCGCGG 1 cut(s) 1397
Csp6I GTAC 6 cut(s) 255, 386, 2237, 2415, 2422, 2439
CspAI ACCGGT 1 cut(s) 2424
CviQI GTAC 6 cut(s) 255, 386, 2237, 2415, 2422, 2439
DpnI GATC 9 cut(s) 456, 653, 762, 783, 1171, 1623, 1711, 1810, 2501
DpnII GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
DraI TTTAAA 1 cut(s) 2191
EaeI YGGCCR 3 cut(s) 774, 1447, 2735
Eam1104I CTCTTC 2 cut(s) 1491, 1523
EarI CTCTTC 2 cut(s) 1491, 1523
Eco130I CCWWGG 5 cut(s) 380, 1096, 1317, 1481, 2922
Eco24I GRGCYC 1 cut(s) 1673
Eco31I GGTCTC 1 cut(s) 2742
Eco32I GATATC 2 cut(s) 1366, 2389
Eco47I GGWCC 4 cut(s) 220, 518, 1321, 1485
Eco47III AGCGCT 1 cut(s) 2186
Eco57I CTGAAG 4 cut(s) 200, 1310, 2049, 2127
Eco81I CCTNAGG 2 cut(s) 598, 2356
EcoRI GAATTC 1 cut(s) 2361
EcoRII CCWGG 5 cut(s) 250, 823, 928, 2239, 2315
EcoRV GATATC 2 cut(s) 1366, 2389
EcoT14I CCWWGG 5 cut(s) 380, 1096, 1317, 1481, 2922
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 1673
ErhI CCWWGG 5 cut(s) 380, 1096, 1317, 1481, 2922
FalI AAGNNNNNCTT 4 cut(s) 581, 613, 1159, 1191
FaqI GGGAC 3 cut(s) 283, 535, 2885
FauI CCCGC 1 cut(s) 1983
FauNDI CATATG 2 cut(s) 1305, 2199
FblI GTMKAC 1 cut(s) 855
Fnu4HI GCNGC 8 cut(s) 120, 1260, 1397, 1521, 1602, 1721, 1724, 2912
FriOI GRGCYC 1 cut(s) 1673
Fsp4HI GCNGC 8 cut(s) 120, 1260, 1397, 1521, 1602, 1721, 1724, 2912
FspBI CTAG 9 cut(s) 129, 389, 416, 1193, 1701, 1967, 2078, 2504, 2589
GlaI GCGC 1 cut(s) 2186
GluI GCNGC 8 cut(s) 120, 1260, 1397, 1521, 1602, 1721, 1724, 2912
GsaI CCCAGC 2 cut(s) 494, 1334
GsuI CTGGAG 3 cut(s) 426, 1578, 2766
HaeII RGCGCY 1 cut(s) 2188
HapII CCGG 7 cut(s) 88, 157, 758, 1636, 1778, 2425, 2578
HhaI GCGC 1 cut(s) 2187
Hin6I GCGC 1 cut(s) 2185
HinP1I GCGC 1 cut(s) 2185
HincII GTYRAC 2 cut(s) 106, 2887
HindII GTYRAC 2 cut(s) 106, 2887
HpaI GTTAAC 1 cut(s) 2887
HpaII CCGG 7 cut(s) 88, 157, 758, 1636, 1778, 2425, 2578
HphI GGTGA 6 cut(s) 226, 929, 1085, 1999, 2303, 2522
Hpy166II GTNNAC 6 cut(s) 106, 856, 2525, 2800, 2827, 2887
Hpy8I GTNNAC 6 cut(s) 106, 856, 2525, 2800, 2827, 2887
Hpy99I CGWCG 1 cut(s) 2710
HpyCH4III ACNGT 6 cut(s) 31, 574, 2005, 2011, 2876, 2884
HpyCH4IV ACGT 3 cut(s) 690, 1408, 2705
HpyF10VI GCNNNNNNNGC 5 cut(s) 653, 1380, 1385, 1598, 2184
HpySE526I ACGT 3 cut(s) 690, 1408, 2705
HspAI GCGC 1 cut(s) 2185
Kpn2I TCCGGA 3 cut(s) 757, 1777, 2577
KpnI GGTACC 1 cut(s) 2240
KspAI GTTAAC 1 cut(s) 2887
KspI CCGCGG 1 cut(s) 1397
Kzo9I GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
Lsp1109I GCAGC 5 cut(s) 106, 1246, 1613, 1707, 1735
LweI GCATC 8 cut(s) 322, 503, 804, 1065, 1146, 1306, 2089, 2686
MaeI CTAG 9 cut(s) 129, 389, 416, 1193, 1701, 1967, 2078, 2504, 2589
MaeII ACGT 3 cut(s) 690, 1408, 2705
MaeIII GTNAC 8 cut(s) 176, 232, 372, 1063, 1535, 1969, 2005, 2959
MalI GATC 9 cut(s) 456, 653, 762, 783, 1171, 1623, 1711, 1810, 2501
MbiI CCGCTC 2 cut(s) 1990, 2911
MboI GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
MflI RGATCY 3 cut(s) 760, 781, 1808
MhlI GDGCHC 2 cut(s) 1594, 1673
MlsI TGGCCA 3 cut(s) 776, 1449, 2737
MluCI AATT 9 cut(s) 480, 678, 958, 985, 998, 1743, 2069, 2154, 2361
MluNI TGGCCA 3 cut(s) 776, 1449, 2737
MlyI GAGTC 4 cut(s) 184, 557, 1908, 2661
MmeI TCCRAC 7 cut(s) 273, 425, 599, 1391, 1402, 2684, 2705
Mox20I TGGCCA 3 cut(s) 776, 1449, 2737
Mph1103I ATGCAT 1 cut(s) 337
MroI TCCGGA 3 cut(s) 757, 1777, 2577
MroXI GAANNNNTTC 1 cut(s) 358
MscI TGGCCA 3 cut(s) 776, 1449, 2737
MslI CAYNNNNRTG 6 cut(s) 442, 792, 1101, 1839, 2135, 2952
Msp20I TGGCCA 3 cut(s) 776, 1449, 2737
MspA1I CMGCKG 4 cut(s) 1049, 1330, 1396, 2432
MspCI CTTAAG 1 cut(s) 2647
MspI CCGG 7 cut(s) 88, 157, 758, 1636, 1778, 2425, 2578
MspR9I CCNGG 7 cut(s) 158, 252, 825, 930, 1636, 2241, 2317
MvaI CCWGG 5 cut(s) 252, 825, 930, 2241, 2317
MvnI CGCG 2 cut(s) 669, 1396
MwoI GCNNNNNNNGC 5 cut(s) 653, 1380, 1385, 1598, 2184
NciI CCSGG 2 cut(s) 158, 1636
NcoI CCATGG 3 cut(s) 1096, 1317, 1481
NdeI CATATG 2 cut(s) 1305, 2199
NdeII GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
NheI GCTAGC 1 cut(s) 1192
NlaIV GGNNCC 8 cut(s) 355, 762, 1671, 1810, 1827, 2139, 2238, 2271
NmeAIII GCCGAG 2 cut(s) 673, 917
NmuCI GTSAC 6 cut(s) 176, 232, 372, 1063, 2005, 2959
NruI TCGCGA 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 3 cut(s) 1844, 2351, 2480
NspV TTCGAA 1 cut(s) 1923
OliI CACNNNNGTG 1 cut(s) 442
PaeI GCATGC 1 cut(s) 2480
PasI CCCWGGG 1 cut(s) 2316
PciI ACATGT 1 cut(s) 2347
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 9 cut(s) 471, 1236, 1631, 1769, 1994, 2492, 2687, 2741, 2927
PflMI CCANNNNNTGG 2 cut(s) 914, 2744
PinAI ACCGGT 1 cut(s) 2424
PkrI GCNGC 8 cut(s) 121, 1261, 1398, 1522, 1603, 1722, 1725, 2913
PleI GAGTC 4 cut(s) 183, 556, 1908, 2660
PpsI GAGTC 4 cut(s) 183, 556, 1908, 2660
PscI ACATGT 1 cut(s) 2347
Psp6I CCWGG 5 cut(s) 250, 823, 928, 2239, 2315
PspFI CCCAGC 2 cut(s) 490, 1330
PspGI CCWGG 5 cut(s) 250, 823, 928, 2239, 2315
PspN4I GGNNCC 8 cut(s) 355, 762, 1671, 1810, 1827, 2139, 2238, 2271
PspOMI GGGCCC 1 cut(s) 1669
PspPI GGNCC 9 cut(s) 220, 518, 594, 932, 1321, 1358, 1485, 1669, 1670
PstI CTGCAG 1 cut(s) 1725
PstNI CAGNNNCTG 1 cut(s) 2744
PsuI RGATCY 3 cut(s) 760, 781, 1808
PvuII CAGCTG 3 cut(s) 1049, 1330, 2432
RruI TCGCGA 1 cut(s) 669
RsaI GTAC 6 cut(s) 256, 387, 2238, 2416, 2423, 2440
RsaNI GTAC 6 cut(s) 255, 386, 2237, 2415, 2422, 2439
RseI CAYNNNNRTG 6 cut(s) 442, 792, 1101, 1839, 2135, 2952
SacII CCGCGG 1 cut(s) 1397
SatI GCNGC 8 cut(s) 120, 1260, 1397, 1521, 1602, 1721, 1724, 2912
Sau3AI GATC 9 cut(s) 454, 651, 760, 781, 1169, 1621, 1709, 1808, 2499
Sau96I GGNCC 9 cut(s) 220, 518, 594, 932, 1321, 1358, 1485, 1669, 1670
SchI GAGTC 4 cut(s) 184, 557, 1908, 2661
ScrFI CCNGG 7 cut(s) 158, 252, 825, 930, 1636, 2241, 2317
SduI GDGCHC 2 cut(s) 1594, 1673
SfaNI GCATC 8 cut(s) 322, 503, 804, 1065, 1146, 1306, 2089, 2686
SfcI CTRYAG 2 cut(s) 30, 1721
Sfr303I CCGCGG 1 cut(s) 1397
SfuI TTCGAA 1 cut(s) 1923
SgrBI CCGCGG 1 cut(s) 1397
SinI GGWCC 4 cut(s) 220, 518, 1321, 1485
SmiMI CAYNNNNRTG 6 cut(s) 442, 792, 1101, 1839, 2135, 2952
SmlI CTYRAG 1 cut(s) 2647
SmoI CTYRAG 1 cut(s) 2647
SpeI ACTAGT 1 cut(s) 388
SphI GCATGC 1 cut(s) 2480
Sse9I AATT 9 cut(s) 480, 678, 958, 985, 998, 1743, 2069, 2154, 2361
SsiI CCGC 7 cut(s) 1394, 1396, 1521, 1990, 2053, 2085, 2911
SspI AATATT 1 cut(s) 1090
SspMI CTAG 9 cut(s) 129, 389, 416, 1193, 1701, 1967, 2078, 2504, 2589
StyD4I CCNGG 7 cut(s) 156, 250, 823, 928, 1634, 2239, 2315
StyI CCWWGG 5 cut(s) 380, 1096, 1317, 1481, 2922
TaaI ACNGT 6 cut(s) 31, 574, 2005, 2011, 2876, 2884
TaiI ACGT 3 cut(s) 693, 1411, 2708
TaqI TCGA 5 cut(s) 60, 741, 1923, 2708, 2835
TasI AATT 9 cut(s) 480, 678, 958, 985, 998, 1743, 2069, 2154, 2361
TatI WGTACW 1 cut(s) 2438
TauI GCSGC 3 cut(s) 1399, 1523, 2914
TfiI GAWTC 9 cut(s) 471, 1236, 1631, 1769, 1994, 2492, 2687, 2741, 2927
TscAI CASTG 5 cut(s) 334, 1471, 2008, 2293, 2873
TseFI GTSAC 6 cut(s) 176, 232, 372, 1063, 2005, 2959
TseI GCWGC 5 cut(s) 119, 1259, 1601, 1720, 1723
Tsp45I GTSAC 6 cut(s) 176, 232, 372, 1063, 2005, 2959
TspGWI ACGGA 2 cut(s) 475, 716
TspRI CASTG 5 cut(s) 334, 1471, 2008, 2293, 2873
Van91I CCANNNNNTGG 2 cut(s) 914, 2744
Vha464I CTTAAG 1 cut(s) 2647
VpaK11BI GGWCC 4 cut(s) 220, 518, 1321, 1485
XapI RAATTY 6 cut(s) 678, 958, 998, 1743, 2154, 2361
XceI RCATGY 3 cut(s) 1844, 2351, 2480
XcmI CCANNNNNNNNNTGG 5 cut(s) 1148, 1457, 1819, 2784, 2873
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 9 cut(s) 129, 389, 416, 1193, 1701, 1967, 2078, 2504, 2589
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.