Rh7AG277000

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005660
Physical Location & Seq
Forward (+)
29048945 .. 29062032
13088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG277000.1

Sequence Viewer

Length: 2976 bp
ATGGCTGGTGATGAAGGGACGCCGGTCACTGCAGATGTCACTTCAGGAGATATGATTTTCGGGCCTATCATTGAGGATGGTGTGTTCCGATTTGATTGTTCTGCAAATGATAGAGAAGCAGCACATCCTAGTCTCTCTTTTGTTAATGGCAAGGATAGGGAGACACCGATTCAGAGTCAGAACATGCCGTCCTATATCCCAAATTTTGAATGTCTTCTTGGGCAGCAGATTGTTAAACTTGAGCTTCCTGTTGGTACCTCTTTATACGGCACTGGGGAGGTTAGCGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAATTCGGATGCATGGGGCTATGGTTCTGGAACTACGTCGTTGTACCAGTCACATCCTTGGGTGCTAGCTGTTCTTCCTTCTGGAGAGGCATTAGGAGTTCTTGCGGATACAACAAAGCGTTGTGAGATTGATTTAAGAAAAGAATCTTTGATACGGTTCATCGCTCCATCCTCTTATCCTGTCATTACTTTTGGTCCATTTGCTACACCCAATGCTGTTTTGAGAGCTTTATCACGTGCAATTGGTACTGTATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAATGTCGCTGGAGCTATGATTCTGATAAGAGAGTTCAAGAGGTTACAAGAACTTTTCGGGAGAAAAAGATTCCTTGTGATGTCGTATGGATGGATATTGATTATATGGATGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAAGGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAAGTGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTCTGGATTTCGAAAGCAGATGGAAAACCTTTTGTTGGGGAGGTGTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAGGCAAAAGTTCGATCGTGGTGGTCCAATTTAGTCAAAGATTTCATTTGTAATGGTGTTGATGGTATATGGAATGACATGAATGAACCAGCTGTTTTTAAGGTTGTCACAAAAACAATGCCTGAGAGCAATATCCATAAGGGGGATGATGAACTGGGAGGCTGCCAGAATCACTCACACTACCACAATGTCTATGGGTTGCTGATGGCAAGATCAACTTATGAAGGGATGAAATTGGGTAATGCAAAGAAACGTCCTTTTGTCCTCACGAGAGCTGGATTTATTGGTAGCCAGCGGTATGCTGCAACATGGACCGGAGACAATCTTTCAACTTGGGAACACCTGCATATGAGTATTCCCATGGTACTTCAATTGGGACTTAGTGGTCAGCCAATGTCGGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTCTTTGGAAGATGGGTGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAAAAGGACACCGCTGACCATGAGCCGTGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACCGCCTTATACCTCATTTATACACTCTCTTCTATATGGCTCATACGACGGGTACTCCAGTGGCAAGTCCTACATTTTTTGCTGATCCCAAAGATACCAGCTTAAGGAAATTGGAGACTTCTTTTCTGTTGGGTCCAGTTCTAGTGTATGCAAGCACTGCGGTTGGTCGGGGGATGGATGCTTTGCAGTGCACATTGCCCAAAGGAATTTGGATGAGTTTCGATTTTGATGATTCACATCCAGATTTACCAGCGTTATATTTGCAAGGTGGAGCAATTATTCCTGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTAATATATTAGATGACTTGACACTCCTTGTGGCTTTAAATGAACATGGGAAAGCTGAAGGTGTTTTATATGAAGATGATGGTGATGGATATGAATTCCTGGAAGGAGGATTCTTGTTGACACACTATGTTGCTGAACTTCAATCATCAACACTTACTCTCAAAATTTCAAAAACTGAAGGATCTTGGAAGAGGCCAAACCGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATATGGGGTAAGGATGGAGAGATTCTTCAACTTTCAGCGCCTTCAGAACAGGAGGTCTCGCAGCTGGTTTCTACCAGTGAGAAACAGTACAGAAGTCGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGTGAGTTCTGCGCAGAAAGGAATAGAGCTTTCAAGGACGCCTATTGAATTGAAAGGTGGTGATTGGGTTATTAAAGTAGTGCCCTGGATTGGTGGTCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACCGAGTACCGCTCTGCTGGATGTTCTGAGGAATACAATGTTATTGAACGAAATCCCGAGCATGCCGGGGAGGAGGAATCTCTCATGTTAGAAGGTGACATAGGGGGTGGATTGGTTCTCCAACGACAGTTCTATATTCCAAAGAATGATCCCAAGGTTTTCCGGATCGACTCTAGCATTATAGCACGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGGCTGGTCTGCTTGAGAGTACACCCGATGTTCACCCTGTTGCACCCCTCAGAATCTTATGTTTCATTTACAGCCATTGACGGGTCAAAGCATGAAATTGGGCCCGAATCTAACGAGCAGTTCTATGAAGGGAATTTAATGCCTAATGGTGAATGGATGCTTATTGATAAATGCCTTGGCTTGGGGTTATTGAATCGGTTTGAGGTCAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATTTGGAGCTGTGGTCCGAGGAGAGGCCTGTTTCAAACCAATCACCTCTTAGAATATCCCATGAGTATGAGGTGATAGATCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

991

Amino Acids

110.5

Weight (kDa)

5.42

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 78 - 152 7.3e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 193 - 522 1.6e-126 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 624 - 692 3.6e-13 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1308
AasI GACNNNNNNGTC 1 cut(s) 1341
Acc16I TGCGCA 1 cut(s) 2244
Acc36I ACCTGC 1 cut(s) 1308
Acc65I GGTACC 2 cut(s) 254, 2414
AccB1I GGYRCC 3 cut(s) 254, 2087, 2414
AccBSI CCGCTC 2 cut(s) 298, 2427
AccIII TCCGGA 1 cut(s) 2577
AciI CCGC 9 cut(s) 285, 298, 428, 1252, 1467, 1516, 1540, 1697, 2425
AclWI GGATC 6 cut(s) 755, 789, 1616, 2041, 2558, 2588
AcsI RAATTY 6 cut(s) 202, 325, 1743, 1946, 2016, 2767
AcuI CTGAAG 5 cut(s) 27, 1929, 2049, 2127, 2340
AcvI CACGTG 1 cut(s) 560
AcyI GRCGYC 2 cut(s) 20, 2270
AflII CTTAAG 1 cut(s) 1639
AjnI CCWGG 4 cut(s) 823, 928, 1950, 2315
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AloI GAACNNNNNNTCC 4 cut(s) 68, 100, 173, 205
Alw21I GWGCWC 2 cut(s) 1401, 1730
Alw26I GTCTC 5 cut(s) 137, 155, 1269, 1646, 2161
Alw44I GTGCAC 1 cut(s) 1726
AlwI GGATC 6 cut(s) 755, 789, 1616, 2041, 2558, 2588
Ama87I CYCGRG 1 cut(s) 2471
Aor13HI TCCGGA 1 cut(s) 2577
AoxI GGCC 7 cut(s) 62, 926, 932, 1358, 2045, 2735, 2912
ApaI GGGCCC 1 cut(s) 2739
ApaLI GTGCAC 1 cut(s) 1726
ApeKI GCWGC 5 cut(s) 119, 223, 1119, 1259, 2161
ApoI RAATTY 6 cut(s) 202, 325, 1743, 1946, 2016, 2767
AseI ATTAAT 1 cut(s) 2391
Asp700I GAANNNNTTC 2 cut(s) 213, 2335
Asp718I GGTACC 2 cut(s) 254, 2414
AspLEI GCGC 3 cut(s) 1536, 2140, 2245
AsuC2I CCSGG 1 cut(s) 2482
AsuII TTCGAA 1 cut(s) 890
AsuNHI GCTAGC 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 2471
AvaII GGWCC 8 cut(s) 518, 594, 748, 981, 1269, 1670, 1826, 2901
BaeGI GKGCMC 4 cut(s) 1452, 1730, 2316, 2739
BaeI ACNNNNGTAYC 2 cut(s) 1581, 1614
BanI GGYRCC 3 cut(s) 254, 2087, 2414
BanII GRGCYC 2 cut(s) 1401, 2739
BauI CACGAG 1 cut(s) 1225
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 1401, 1730
BbvI GCAGC 5 cut(s) 131, 235, 1106, 1246, 2173
BceAI ACGGC 4 cut(s) 172, 283, 1428, 1465
BciT130I CCWGG 4 cut(s) 825, 930, 1952, 2317
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 1 cut(s) 2482
BcoDI GTCTC 5 cut(s) 137, 155, 1269, 1646, 2161
BfaI CTAG 4 cut(s) 129, 389, 1679, 2589
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 2 cut(s) 1537, 2141
BfrI CTTAAG 1 cut(s) 1639
BfuAI ACCTGC 1 cut(s) 1308
BfuI GTATCC 1 cut(s) 424
BglII AGATCT 1 cut(s) 2965
BisI GCNGC 6 cut(s) 120, 224, 1120, 1260, 1516, 2162
BlsI GCNGC 6 cut(s) 121, 225, 1121, 1261, 1517, 2163
Bme1390I CCNGG 5 cut(s) 825, 930, 1952, 2317, 2482
Bme18I GGWCC 8 cut(s) 518, 594, 748, 981, 1269, 1670, 1826, 2901
BmeT110I CYCGRG 1 cut(s) 2471
BmiI GGNNCC 7 cut(s) 256, 1359, 1671, 1827, 2089, 2416, 2737
BmrFI CCNGG 5 cut(s) 825, 930, 1952, 2317, 2482
BmrI ACTGGG 3 cut(s) 282, 1121, 2878
BmsI GCATC 4 cut(s) 322, 804, 1705, 2781
BmtI GCTAGC 1 cut(s) 392
BmuI ACTGGG 3 cut(s) 282, 1121, 2878
BoxI GACNNNNGTC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 2411, 2443
BpmI CTGGAG 3 cut(s) 426, 640, 1578
Bpu10I CCTNAGC 1 cut(s) 1395
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 3 cut(s) 260, 314, 2668
BpuMI CCSGG 1 cut(s) 2482
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 1773
BsaHI GRCGYC 2 cut(s) 20, 2270
BsaI GGTCTC 1 cut(s) 2161
BsaWI WCCGGW 2 cut(s) 1271, 2577
BsaXI ACNNNNNCTCC 4 cut(s) 1576, 1606, 2885, 2915
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 8 cut(s) 277, 370, 1116, 1595, 1673, 2175, 2873, 2881
Bse3DI GCAATG 1 cut(s) 1730
Bse8I GATNNNNATC 1 cut(s) 1773
BseAI TCCGGA 1 cut(s) 2577
BseBI CCWGG 4 cut(s) 825, 930, 1952, 2317
BseJI GATNNNNATC 1 cut(s) 1773
BseMI GCAATG 1 cut(s) 1730
BseMII CTCAG 4 cut(s) 1071, 1386, 2433, 2697
BseNI ACTGG 8 cut(s) 277, 370, 1116, 1595, 1673, 2175, 2873, 2881
BseRI GAGGAG 4 cut(s) 1543, 1821, 2501, 2921
BseSI GKGCMC 4 cut(s) 1452, 1730, 2316, 2739
BseXI GCAGC 5 cut(s) 131, 235, 1106, 1246, 2173
Bsh1285I CGRYCG 1 cut(s) 974
BshFI GGCC 7 cut(s) 64, 928, 934, 1360, 2047, 2737, 2914
BshNI GGYRCC 3 cut(s) 254, 2087, 2414
BsiEI CGRYCG 1 cut(s) 974
BsiHKAI GWGCWC 2 cut(s) 1401, 1730
BsiHKCI CYCGRG 1 cut(s) 2471
BsiSI CCGG 4 cut(s) 23, 1272, 2481, 2578
BslFI GGGAC 3 cut(s) 31, 1347, 2374
BsmAI GTCTC 5 cut(s) 137, 155, 1269, 1646, 2161
BsmFI GGGAC 3 cut(s) 31, 1347, 2374
BsnI GGCC 7 cut(s) 64, 928, 934, 1360, 2047, 2737, 2914
Bso31I GGTCTC 1 cut(s) 2161
BsoBI CYCGRG 1 cut(s) 2471
Bsp119I TTCGAA 1 cut(s) 890
Bsp120I GGGCCC 1 cut(s) 2735
Bsp1286I GDGCHC 5 cut(s) 1401, 1452, 1730, 2316, 2739
Bsp13I TCCGGA 1 cut(s) 2577
Bsp143I GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
Bsp19I CCATGG 2 cut(s) 1317, 2091
BspACI CCGC 9 cut(s) 285, 298, 428, 1252, 1467, 1516, 1540, 1697, 2425
BspANI GGCC 7 cut(s) 64, 928, 934, 1360, 2047, 2737, 2914
BspCNI CTCAG 4 cut(s) 1072, 1387, 2434, 2696
BspEI TCCGGA 1 cut(s) 2577
BspLI GGNNCC 7 cut(s) 256, 1359, 1671, 1827, 2089, 2416, 2737
BspMAI CTGCAG 1 cut(s) 34
BspMI ACCTGC 1 cut(s) 1308
BspOI GCTAGC 1 cut(s) 392
BspPI GGATC 6 cut(s) 755, 789, 1616, 2041, 2558, 2588
BspT104I TTCGAA 1 cut(s) 890
BspT107I GGYRCC 3 cut(s) 254, 2087, 2414
BspTI CTTAAG 1 cut(s) 1639
BspTNI GGTCTC 1 cut(s) 2161
BsrBI CCGCTC 2 cut(s) 298, 2427
BsrDI GCAATG 1 cut(s) 1730
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 8 cut(s) 277, 370, 1116, 1595, 1673, 2175, 2873, 2881
BssAI RCCGGY 1 cut(s) 22
BssMI GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
BssNI GRCGYC 2 cut(s) 20, 2270
BssSI CACGAG 1 cut(s) 1225
BssT1I CCWWGG 7 cut(s) 380, 597, 771, 1317, 2091, 2568, 2809
Bst2BI CACGAG 1 cut(s) 1225
Bst2UI CCWGG 4 cut(s) 825, 930, 1952, 2317
Bst4CI ACNGT 9 cut(s) 480, 574, 752, 2054, 2187, 2367, 2378, 2544, 2884
Bst6I CTCTTC 3 cut(s) 1491, 1571, 2036
BstACI GRCGYC 2 cut(s) 20, 2270
BstAFI CTTAAG 1 cut(s) 1639
BstAPI GCANNNNNTGC 2 cut(s) 1694, 2074
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 7 cut(s) 287, 390, 1250, 1690, 2060, 2478, 2602
BstDEI CTNAG 6 cut(s) 1080, 1337, 1395, 2442, 2683, 2936
BstDSI CCRYGG 4 cut(s) 1317, 1444, 1481, 2091
BstH2I RGCGCY 2 cut(s) 1537, 2141
BstHHI GCGC 3 cut(s) 1536, 2140, 2245
BstKTI GATC 9 cut(s) 763, 784, 974, 1172, 1624, 2036, 2566, 2583, 2968
BstMAI GTCTC 5 cut(s) 137, 155, 1269, 1646, 2161
BstMBI GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
BstMCI CGRYCG 1 cut(s) 974
BstMWI GCNNNNNNNGC 4 cut(s) 295, 1385, 1694, 2074
BstNI CCWGG 4 cut(s) 825, 930, 1952, 2317
BstNSI RCATGY 4 cut(s) 187, 1844, 2066, 2480
BstPAI GACNNNNGTC 1 cut(s) 23
BstSCI CCNGG 5 cut(s) 823, 928, 1950, 2315, 2480
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 4 cut(s) 1452, 1730, 2316, 2739
BstV1I GCAGC 5 cut(s) 131, 235, 1106, 1246, 2173
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 4 cut(s) 760, 781, 2033, 2965
BstXI CCANNNNNNTGG 1 cut(s) 794
BstYI RGATCY 4 cut(s) 760, 781, 2033, 2965
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 7 cut(s) 64, 928, 934, 1360, 2047, 2737, 2914
BtgI CCRYGG 4 cut(s) 1317, 1444, 1481, 2091
BtgZI GCGATG 1 cut(s) 469
BtsI GCAGTG 3 cut(s) 27, 1692, 1730
BtsIMutI CAGTG 8 cut(s) 27, 270, 1602, 1692, 1730, 2182, 2372, 2866
BveI ACCTGC 1 cut(s) 1308
Cac8I GCNNGC 7 cut(s) 287, 390, 1250, 1690, 2060, 2478, 2602
CfoI GCGC 3 cut(s) 1536, 2140, 2245
Cfr10I RCCGGY 1 cut(s) 22
CseI GACGC 2 cut(s) 28, 2278
DdeI CTNAG 6 cut(s) 1080, 1337, 1395, 2442, 2683, 2936
DpnI GATC 9 cut(s) 762, 783, 973, 1171, 1623, 2035, 2565, 2582, 2967
DpnII GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
DraI TTTAAA 1 cut(s) 1890
DrdI GACNNNNNNGTC 1 cut(s) 1341
DseDI GACNNNNNNGTC 1 cut(s) 1341
Eam1104I CTCTTC 3 cut(s) 1491, 1571, 2036
EarI CTCTTC 3 cut(s) 1491, 1571, 2036
Ecl136II GAGCTC 1 cut(s) 1399
Eco130I CCWWGG 7 cut(s) 380, 597, 771, 1317, 2091, 2568, 2809
Eco147I AGGCCT 1 cut(s) 2914
Eco24I GRGCYC 2 cut(s) 1401, 2739
Eco31I GGTCTC 1 cut(s) 2161
Eco47I GGWCC 8 cut(s) 518, 594, 748, 981, 1269, 1670, 1826, 2901
Eco53kI GAGCTC 1 cut(s) 1399
Eco57I CTGAAG 5 cut(s) 27, 1929, 2049, 2127, 2340
Eco72I CACGTG 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 2471
EcoICRI GAGCTC 1 cut(s) 1399
EcoO109I RGGNCCY 2 cut(s) 1358, 1826
EcoRI GAATTC 2 cut(s) 325, 1946
EcoRII CCWGG 4 cut(s) 823, 928, 1950, 2315
EcoT14I CCWWGG 7 cut(s) 380, 597, 771, 1317, 2091, 2568, 2809
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 1401, 2739
ErhI CCWWGG 7 cut(s) 380, 597, 771, 1317, 2091, 2568, 2809
FalI AAGNNNNNCTT 4 cut(s) 581, 613, 1159, 1191
FaqI GGGAC 3 cut(s) 31, 1347, 2374
FauI CCCGC 1 cut(s) 278
FauNDI CATATG 1 cut(s) 1305
Fnu4HI GCNGC 6 cut(s) 120, 224, 1120, 1260, 1516, 2162
FriOI GRGCYC 2 cut(s) 1401, 2739
Fsp4HI GCNGC 6 cut(s) 120, 224, 1120, 1260, 1516, 2162
FspBI CTAG 4 cut(s) 129, 389, 1679, 2589
FspI TGCGCA 1 cut(s) 2244
GlaI GCGC 3 cut(s) 1535, 2139, 2244
GluI GCNGC 6 cut(s) 120, 224, 1120, 1260, 1516, 2162
GsuI CTGGAG 3 cut(s) 426, 640, 1578
HaeII RGCGCY 2 cut(s) 1537, 2141
HaeIII GGCC 7 cut(s) 64, 928, 934, 1360, 2047, 2737, 2914
HapII CCGG 4 cut(s) 23, 1272, 2481, 2578
HgaI GACGC 2 cut(s) 28, 2278
HhaI GCGC 3 cut(s) 1536, 2140, 2245
Hin1I GRCGYC 2 cut(s) 20, 2270
Hin6I GCGC 3 cut(s) 1534, 2138, 2243
HinP1I GCGC 3 cut(s) 1534, 2138, 2243
HincII GTYRAC 1 cut(s) 1971
HindII GTYRAC 1 cut(s) 1971
HpaII CCGG 4 cut(s) 23, 1272, 2481, 2578
Hpy166II GTNNAC 4 cut(s) 1728, 1971, 2656, 2667
Hpy8I GTNNAC 4 cut(s) 1728, 1971, 2656, 2667
Hpy99I CGWCG 3 cut(s) 364, 1588, 2058
HpyCH4III ACNGT 9 cut(s) 480, 574, 752, 2054, 2187, 2367, 2378, 2544, 2884
HpyCH4IV ACGT 3 cut(s) 359, 559, 1210
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 1385, 1694, 2074
HpyF3I CTNAG 6 cut(s) 1080, 1337, 1395, 2442, 2683, 2936
HpySE526I ACGT 3 cut(s) 359, 559, 1210
Hsp92I GRCGYC 2 cut(s) 20, 2270
HspAI GCGC 3 cut(s) 1534, 2138, 2243
Kpn2I TCCGGA 1 cut(s) 2577
KpnI GGTACC 2 cut(s) 258, 2418
Kzo9I GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
LmnI GCTCC 4 cut(s) 493, 621, 1808, 2893
Lsp1109I GCAGC 5 cut(s) 131, 235, 1106, 1246, 2173
LweI GCATC 4 cut(s) 322, 804, 1705, 2781
MaeI CTAG 4 cut(s) 129, 389, 1679, 2589
MaeII ACGT 3 cut(s) 359, 559, 1210
MaeIII GTNAC 6 cut(s) 25, 37, 372, 652, 1063, 2510
MalI GATC 9 cut(s) 762, 783, 973, 1171, 1623, 2035, 2565, 2582, 2967
MbiI CCGCTC 2 cut(s) 298, 2427
MboI GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
MfeI CAATTG 2 cut(s) 564, 1328
MflI RGATCY 4 cut(s) 760, 781, 2033, 2965
MhlI GDGCHC 5 cut(s) 1401, 1452, 1730, 2316, 2739
MlyI GAGTC 3 cut(s) 184, 2338, 2579
MmeI TCCRAC 1 cut(s) 2560
Mph1103I ATGCAT 1 cut(s) 337
MroI TCCGGA 1 cut(s) 2577
MroXI GAANNNNTTC 2 cut(s) 213, 2335
MslI CAYNNNNRTG 5 cut(s) 792, 1839, 1845, 2339, 2952
MspA1I CMGCKG 4 cut(s) 1049, 1252, 1469, 2164
MspCI CTTAAG 1 cut(s) 1639
MspI CCGG 4 cut(s) 23, 1272, 2481, 2578
MspR9I CCNGG 5 cut(s) 825, 930, 1952, 2317, 2482
MunI CAATTG 2 cut(s) 564, 1328
MvaI CCWGG 4 cut(s) 825, 930, 1952, 2317
MwoI GCNNNNNNNGC 4 cut(s) 295, 1385, 1694, 2074
NciI CCSGG 1 cut(s) 2482
NcoI CCATGG 2 cut(s) 1317, 2091
NdeI CATATG 1 cut(s) 1305
NdeII GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
NheI GCTAGC 1 cut(s) 388
NlaIV GGNNCC 7 cut(s) 256, 1359, 1671, 1827, 2089, 2416, 2737
NmuCI GTSAC 5 cut(s) 25, 37, 372, 1063, 2510
NsbI TGCGCA 1 cut(s) 2244
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 4 cut(s) 187, 1844, 2066, 2480
NspV TTCGAA 1 cut(s) 890
PaeI GCATGC 1 cut(s) 2480
PaqCI CACCTGC 1 cut(s) 1308
PasI CCCWGGG 1 cut(s) 824
PceI AGGCCT 1 cut(s) 2914
PdmI GAANNNNTTC 2 cut(s) 213, 2335
PfoI TCCNGGA 1 cut(s) 1950
PkrI GCNGC 6 cut(s) 121, 225, 1121, 1261, 1517, 2163
Ple19I CGATCG 1 cut(s) 974
PleI GAGTC 3 cut(s) 183, 2338, 2579
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 3 cut(s) 183, 2338, 2579
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 1826
PshAI GACNNNNGTC 1 cut(s) 23
PshBI ATTAAT 1 cut(s) 2391
Psp124BI GAGCTC 1 cut(s) 1401
Psp5II RGGWCCY 1 cut(s) 1826
Psp6I CCWGG 4 cut(s) 823, 928, 1950, 2315
PspCI CACGTG 1 cut(s) 560
PspGI CCWGG 4 cut(s) 823, 928, 1950, 2315
PspN4I GGNNCC 7 cut(s) 256, 1359, 1671, 1827, 2089, 2416, 2737
PspOMI GGGCCC 1 cut(s) 2735
PspPPI RGGWCCY 1 cut(s) 1826
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 4 cut(s) 760, 781, 2033, 2965
PvuI CGATCG 1 cut(s) 974
PvuII CAGCTG 2 cut(s) 1049, 2164
RseI CAYNNNNRTG 5 cut(s) 792, 1839, 1845, 2339, 2952
SacI GAGCTC 1 cut(s) 1401
SatI GCNGC 6 cut(s) 120, 224, 1120, 1260, 1516, 2162
Sau3AI GATC 9 cut(s) 760, 781, 971, 1169, 1621, 2033, 2563, 2580, 2965
SchI GAGTC 3 cut(s) 184, 2338, 2579
ScrFI CCNGG 5 cut(s) 825, 930, 1952, 2317, 2482
SduI GDGCHC 5 cut(s) 1401, 1452, 1730, 2316, 2739
SfaNI GCATC 4 cut(s) 322, 804, 1705, 2781
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 890
SinI GGWCC 8 cut(s) 518, 594, 748, 981, 1269, 1670, 1826, 2901
SmiMI CAYNNNNRTG 5 cut(s) 792, 1839, 1845, 2339, 2952
SmlI CTYRAG 4 cut(s) 239, 293, 1639, 2647
SmoI CTYRAG 4 cut(s) 239, 293, 1639, 2647
SphI GCATGC 1 cut(s) 2480
SseBI AGGCCT 1 cut(s) 2914
SsiI CCGC 9 cut(s) 285, 298, 428, 1252, 1467, 1516, 1540, 1697, 2425
SspMI CTAG 4 cut(s) 129, 389, 1679, 2589
SstI GAGCTC 1 cut(s) 1401
StuI AGGCCT 1 cut(s) 2914
StyD4I CCNGG 5 cut(s) 823, 928, 1950, 2315, 2480
StyI CCWWGG 7 cut(s) 380, 597, 771, 1317, 2091, 2568, 2809
TaaI ACNGT 9 cut(s) 480, 574, 752, 2054, 2187, 2367, 2378, 2544, 2884
TaiI ACGT 3 cut(s) 362, 562, 1213
TaqI TCGA 5 cut(s) 890, 970, 1758, 2329, 2583
TatI WGTACW 2 cut(s) 2187, 2653
TauI GCSGC 1 cut(s) 1518
TscAI CASTG 8 cut(s) 34, 277, 1602, 1699, 1730, 2182, 2372, 2873
TseFI GTSAC 5 cut(s) 25, 37, 372, 1063, 2510
TseI GCWGC 5 cut(s) 119, 223, 1119, 1259, 2161
Tsp45I GTSAC 5 cut(s) 25, 37, 372, 1063, 2510
TspRI CASTG 8 cut(s) 34, 277, 1602, 1699, 1730, 2182, 2372, 2873
Vha464I CTTAAG 1 cut(s) 1639
VneI GTGCAC 1 cut(s) 1726
VpaK11BI GGWCC 8 cut(s) 518, 594, 748, 981, 1269, 1670, 1826, 2901
VspI ATTAAT 1 cut(s) 2391
XapI RAATTY 6 cut(s) 202, 325, 1743, 1946, 2016, 2767
XceI RCATGY 4 cut(s) 187, 1844, 2066, 2480
XcmI CCANNNNNNNNNTGG 2 cut(s) 1148, 2873
XmnI GAANNNNTTC 2 cut(s) 213, 2335
XspI CTAG 4 cut(s) 129, 389, 1679, 2589
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.