Rh7BG271100

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005660
Physical Location & Seq
Forward (+)
24919291 .. 24933075
13785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG271100.1

Sequence Viewer

Length: 3210 bp
ATGGGAGAAGCACCAGTGCTCACTGCCACACTAGTAGGTGAAGCATCCTCCAAATTCACCCGACGATTTCCAAGTACCACTTCCATTTTTGACACCAACTCTTACAGTGCCGCTCATCCAACCCCGATTTCGTTGCTGAGGTTGAACAAGAAGCCTGGTTCTCAGTCTGCCTTCATCAGAAAGAAGTGTACTAAGTTGGTTACGAAAAGTTTGATATCCAAAATGGCTGGTGATGAAGGGACGCCGGTCACTGCAGATGTCACTTCAGGAGATATGATTTTCGGGCCTATTATTGAGGATGGTGTGTTCCGATTTGATTGTTCTGCAAATGATAGAGAAGCAGCACATCCTAGTCTCTCTTTTGTGAATGGGAAGGATAGGGAGACACCGATTCAGAGTCAAAACATGCCGTCCTATATCCCAAATTTTGAACGTCTTCTTGGGCAGCAGATTGTTAAACTTGAGCTTCCTGTTGGTACCTCTTTATACGGCACTGGGGAGGTTAGCGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAATTCGGATGCATGGGGCTATGGTTCTGGAACTACATCATTGTACCAGTCACATCCTTGGGTGCTAGCTGTTCTTCCTTCTGGAGAGGCATTAGGAGTTCTTGCTGATACAACAAAGCGTTGTGAGATTGATTTAAGAAAAGAATCTTTGATACGGTTCATCGCTCCATCCTCTTATCCGGTCATTACTTTTGGTCCATTTGCTACACCCAATGCTGTTTTGAGAGCTTTATCACGTGCAATTGGTACTGTATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAATGTCGCTGGAGCTATGACTCTGATAAGAGAGTTCAAGAGGTTACAAGAACTTTTCGGGAGAAAAAGATTCCTTGTGATGTCGTATGGATGGATATTGATTATATGGATGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAAGGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAAGTGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTCTGGATTTCGAAAGCAGATGGAAAACCTTTTGTTGGGGAGGTGTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAGGCAAAAGTTCGATCGTGGTGGTCCAATTTAGTCAAAGATTTCATTTGTAATGGTGTTGATGGTATATGGAATGACATGAATGAACCAGCTGTTTTTAAGGTTGTCACAAAAACAATGCCTGAGAGCAATATCCATAAGGGGGATGATGAACTGGGAGGCTGCCAGAATCACTCACACTACCACAATGTCTATGGGTTGCTGATGGCAAGATCAACTTATGAAGGGATGAAATTGGGTAATGCAAAGAAACGTCCTTTTGTCCTCACGAGAGCTGGATTTATTGGTAGCCAGCGGTATGCTGCAACATGGACCGGAGACAATCTTTCAACTTGGGAACACCTGCATATGAGTATTCCCATGGTACTTCAATTGGGACTTAGTGGTCAGCCAATGTCGGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTCTTTGGAAGATGGGTGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAACAGGACACCGCTGACCATGAGCCGTGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACCGCCTTATACCTCATTTATACACTCTCTTCTATATGGCTCATACGACGGGTACTCCAGTGGCAAGTCCTACATTTTTTGCTGATTCCAAAGATACCAGCTTAAGGAAACTGGAGACTTCTTTTTTGTTGGGTCCAGTTCTAGTGTATGCAAGCACTGCGGTTGGTCGGGGGATGGATGGTTTGCAGTGCACATTGCCCAAAGGAATCTGGATGAGTTTCGATTTTGATGATTCACATCCAGATTTACCAGCGTTATATTTGCAAGGTGGAGCAATTATTCCTGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTAATATATTAGATGACTTGACACTCCTTGTGGCTTTAAATGAACATGGGAAAGCTGAAGGTGTTTTATATGAAGATGATGGTGATGGATATGAATTCCTGGAAGGAGGATTCTTGTTGACACACTATGTTGCTGAACTTCAATCATCAACACTTACTCTCAAAATTTCAAAAACTGAAGGATCTTGGAAGAGACCAAACCGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATATGGGGTAAGGATGGAGAGATTCTTCAACTTTCAACGCCTTCAGAACAGGAGGTCTCGCAGCTGGTTTCTACCAGTGAGAAACAGTACAGAACTCGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGTGAGTTCTGCGCAGAAAGGAATAGAGCTTTCAAGGACGCCTATTGAATTGAAAGGTGGTGATTGGGTTATTAAAGTAGTGCCCTGGATTGGTGGTCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACCGAGTACCGCTCTGCTGGATGTTCCGAGGAATACAATGTTATTGATCTCCCTCGCAGACGAAATCTCGAGCATGCCGGGGAGGAGGAATCTCTCATGTTAGAAGGTGATATAGGGGGTGGATTGGTTCTCCAACGACAGTTATATATTCCAAAGAATGATCCCAAGGTTTTCCGGATCGACTCTAGCATTATAGCACGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGACTGGTCTGCTTGAGAGTACACCCGATGTTCACCCTGTTGCACCCCTCAGAATCTTATGTTTCATTTACGGCCATTGACGGGTCAAAGCATGAAATTGGGCCCGAATCTAACGAGCAGTTCTATGAAGGGAATTTAATGCCTAATGGTGAATGGATGCTCATTGATAAATGCCTTGGCTTGGGGTTATTGAATCGGTTTGATGTCAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATTTGGAGCTGTGGTCCGAGGAGAGGCCTGTTTCAAACCAATCACCTCTTAGAATATCCCATGAGTATGAGGTGATAGATCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1069

Amino Acids

119.05

Weight (kDa)

5.87

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 152 - 226 7.3e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 267 - 596 2.7e-126 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 698 - 766 3.9e-13 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1530
AasI GACNNNNNNGTC 2 cut(s) 1563, 2274
Acc16I TGCGCA 1 cut(s) 2466
Acc36I ACCTGC 1 cut(s) 1530
Acc65I GGTACC 2 cut(s) 476, 2636
AccB1I GGYRCC 3 cut(s) 476, 2309, 2636
AccBSI CCGCTC 3 cut(s) 113, 520, 2649
AccIII TCCGGA 1 cut(s) 2811
AciI CCGC 9 cut(s) 111, 507, 520, 1474, 1689, 1738, 1762, 1919, 2647
AclWI GGATC 5 cut(s) 977, 1011, 2263, 2792, 2822
AcoI YGGCCR 1 cut(s) 2940
AcsI RAATTY 6 cut(s) 53, 424, 547, 2168, 2238, 3001
AcuI CTGAAG 5 cut(s) 249, 2151, 2271, 2349, 2562
AcvI CACGTG 1 cut(s) 782
AcyI GRCGYC 2 cut(s) 242, 2492
AflII CTTAAG 1 cut(s) 1861
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
AjuI GAANNNNNNNTTGG 6 cut(s) 64, 96, 112, 144, 423, 455
AloI GAACNNNNNNTCC 2 cut(s) 290, 322
Alw21I GWGCWC 3 cut(s) 21, 1623, 1952
Alw26I GTCTC 6 cut(s) 359, 377, 1491, 1868, 2260, 2383
Alw44I GTGCAC 1 cut(s) 1948
AlwI GGATC 5 cut(s) 977, 1011, 2263, 2792, 2822
Ama87I CYCGRG 1 cut(s) 2705
Aor13HI TCCGGA 1 cut(s) 2811
AoxI GGCC 7 cut(s) 284, 1148, 1154, 1580, 2940, 2969, 3146
ApaI GGGCCC 1 cut(s) 2973
ApaLI GTGCAC 1 cut(s) 1948
ApeKI GCWGC 5 cut(s) 341, 445, 1341, 1481, 2383
ApoI RAATTY 6 cut(s) 53, 424, 547, 2168, 2238, 3001
ArsI GACNNNNNNTTYG 2 cut(s) 1867, 1899
AseI ATTAAT 1 cut(s) 2613
Asp700I GAANNNNTTC 2 cut(s) 435, 2557
Asp718I GGTACC 2 cut(s) 476, 2636
AspLEI GCGC 2 cut(s) 1758, 2467
AsuC2I CCSGG 1 cut(s) 2716
AsuII TTCGAA 1 cut(s) 1112
AsuNHI GCTAGC 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 2705
AvaII GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3135
BaeGI GKGCMC 4 cut(s) 1674, 1952, 2538, 2973
BaeI ACNNNNGTAYC 2 cut(s) 1803, 1836
BanI GGYRCC 3 cut(s) 476, 2309, 2636
BanII GRGCYC 2 cut(s) 1623, 2973
BauI CACGAG 1 cut(s) 1447
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 3 cut(s) 21, 1623, 1952
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
BceAI ACGGC 5 cut(s) 394, 505, 1650, 1687, 2955
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BcnI CCSGG 1 cut(s) 2716
BcoDI GTCTC 6 cut(s) 359, 377, 1491, 1868, 2260, 2383
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 5 cut(s) 32, 351, 611, 1901, 2823
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 1759
BfrI CTTAAG 1 cut(s) 1861
BfuAI ACCTGC 1 cut(s) 1530
BglII AGATCT 1 cut(s) 3199
BisI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
BlsI GCNGC 7 cut(s) 112, 343, 447, 1343, 1483, 1739, 2385
Bme1390I CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2716
Bme18I GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3135
BmeT110I CYCGRG 1 cut(s) 2705
BmiI GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2971
BmrFI CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2716
BmrI ACTGGG 3 cut(s) 504, 1343, 3112
BmsI GCATC 4 cut(s) 53, 544, 1026, 3015
BmtI GCTAGC 1 cut(s) 614
BmuI ACTGGG 3 cut(s) 504, 1343, 3112
BoxI GACNNNNGTC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 2633, 2665
BpmI CTGGAG 4 cut(s) 648, 862, 1800, 1892
Bpu10I CCTNAGC 2 cut(s) 137, 1617
Bpu14I TTCGAA 1 cut(s) 1112
BpuEI CTTGAG 3 cut(s) 482, 536, 2902
BpuMI CCSGG 1 cut(s) 2716
BsaAI YACGTR 1 cut(s) 782
BsaBI GATNNNNATC 1 cut(s) 1995
BsaHI GRCGYC 2 cut(s) 242, 2492
BsaI GGTCTC 2 cut(s) 2260, 2383
BsaWI WCCGGW 3 cut(s) 724, 1493, 2811
BsaXI ACNNNNNCTCC 4 cut(s) 1798, 1828, 3119, 3149
Bse118I RCCGGY 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 1952
Bse8I GATNNNNATC 1 cut(s) 1995
BseAI TCCGGA 1 cut(s) 2811
BseBI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BseJI GATNNNNATC 1 cut(s) 1995
BseMI GCAATG 1 cut(s) 1952
BseMII CTCAG 5 cut(s) 128, 176, 1293, 1608, 2931
BseRI GAGGAG 4 cut(s) 1765, 2043, 2735, 3155
BseSI GKGCMC 4 cut(s) 1674, 1952, 2538, 2973
BseXI GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2942, 2971, 3148
BshNI GGYRCC 3 cut(s) 476, 2309, 2636
BsiEI CGRYCG 1 cut(s) 1196
BsiHKAI GWGCWC 3 cut(s) 21, 1623, 1952
BsiHKCI CYCGRG 1 cut(s) 2705
BsiSI CCGG 5 cut(s) 245, 725, 1494, 2715, 2812
BslFI GGGAC 3 cut(s) 253, 1569, 2596
BsmAI GTCTC 6 cut(s) 359, 377, 1491, 1868, 2260, 2383
BsmFI GGGAC 3 cut(s) 253, 1569, 2596
BsnI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2942, 2971, 3148
Bso31I GGTCTC 2 cut(s) 2260, 2383
BsoBI CYCGRG 1 cut(s) 2705
Bsp119I TTCGAA 1 cut(s) 1112
Bsp120I GGGCCC 1 cut(s) 2969
Bsp1286I GDGCHC 6 cut(s) 21, 1623, 1674, 1952, 2538, 2973
Bsp13I TCCGGA 1 cut(s) 2811
Bsp143I GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
Bsp19I CCATGG 2 cut(s) 1539, 2313
BspACI CCGC 9 cut(s) 111, 507, 520, 1474, 1689, 1738, 1762, 1919, 2647
BspANI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2942, 2971, 3148
BspCNI CTCAG 5 cut(s) 129, 175, 1294, 1609, 2930
BspEI TCCGGA 1 cut(s) 2811
BspLI GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2971
BspMAI CTGCAG 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 1530
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 5 cut(s) 977, 1011, 2263, 2792, 2822
BspT104I TTCGAA 1 cut(s) 1112
BspT107I GGYRCC 3 cut(s) 476, 2309, 2636
BspTI CTTAAG 1 cut(s) 1861
BspTNI GGTCTC 2 cut(s) 2260, 2383
BsrBI CCGCTC 3 cut(s) 113, 520, 2649
BsrDI GCAATG 1 cut(s) 1952
BsrFI RCCGGY 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 244
BssMI GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
BssNI GRCGYC 2 cut(s) 242, 2492
BssSI CACGAG 1 cut(s) 1447
BssT1I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2802, 3043
Bst2BI CACGAG 1 cut(s) 1447
Bst2UI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
Bst6I CTCTTC 3 cut(s) 1713, 1793, 2258
BstACI GRCGYC 2 cut(s) 242, 2492
BstAFI CTTAAG 1 cut(s) 1861
BstAPI GCANNNNNTGC 2 cut(s) 1916, 2296
BstBAI YACGTR 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 7 cut(s) 509, 612, 1472, 1912, 2282, 2712, 2836
BstDEI CTNAG 8 cut(s) 137, 162, 192, 1302, 1559, 1617, 2917, 3170
BstDSI CCRYGG 4 cut(s) 1539, 1666, 1703, 2313
BstH2I RGCGCY 1 cut(s) 1759
BstHHI GCGC 2 cut(s) 1758, 2467
BstKTI GATC 9 cut(s) 985, 1006, 1196, 1394, 2258, 2686, 2800, 2817, 3202
BstMAI GTCTC 6 cut(s) 359, 377, 1491, 1868, 2260, 2383
BstMBI GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
BstNI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BstNSI RCATGY 4 cut(s) 409, 2066, 2288, 2714
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 6 cut(s) 154, 1045, 1150, 2172, 2537, 2714
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 4 cut(s) 1674, 1952, 2538, 2973
BstV1I GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 4 cut(s) 982, 1003, 2255, 3199
BstXI CCANNNNNNTGG 1 cut(s) 1016
BstYI RGATCY 4 cut(s) 982, 1003, 2255, 3199
BsuRI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2942, 2971, 3148
BtgI CCRYGG 4 cut(s) 1539, 1666, 1703, 2313
BtgZI GCGATG 1 cut(s) 691
BtsI GCAGTG 4 cut(s) 21, 249, 1914, 1952
BveI ACCTGC 1 cut(s) 1530
Cac8I GCNNGC 7 cut(s) 509, 612, 1472, 1912, 2282, 2712, 2836
CfoI GCGC 2 cut(s) 1758, 2467
Cfr10I RCCGGY 1 cut(s) 244
CseI GACGC 2 cut(s) 250, 2500
DdeI CTNAG 8 cut(s) 137, 162, 192, 1302, 1559, 1617, 2917, 3170
DpnI GATC 9 cut(s) 984, 1005, 1195, 1393, 2257, 2685, 2799, 2816, 3201
DpnII GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
DraI TTTAAA 1 cut(s) 2112
DrdI GACNNNNNNGTC 2 cut(s) 1563, 2274
DseDI GACNNNNNNGTC 2 cut(s) 1563, 2274
EaeI YGGCCR 1 cut(s) 2940
Eam1104I CTCTTC 3 cut(s) 1713, 1793, 2258
EarI CTCTTC 3 cut(s) 1713, 1793, 2258
Ecl136II GAGCTC 1 cut(s) 1621
Eco130I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2802, 3043
Eco147I AGGCCT 1 cut(s) 3148
Eco24I GRGCYC 2 cut(s) 1623, 2973
Eco31I GGTCTC 2 cut(s) 2260, 2383
Eco32I GATATC 1 cut(s) 216
Eco47I GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3135
Eco53kI GAGCTC 1 cut(s) 1621
Eco57I CTGAAG 5 cut(s) 249, 2151, 2271, 2349, 2562
Eco72I CACGTG 1 cut(s) 782
Eco88I CYCGRG 1 cut(s) 2705
EcoICRI GAGCTC 1 cut(s) 1621
EcoO109I RGGNCCY 2 cut(s) 1580, 2048
EcoRI GAATTC 2 cut(s) 547, 2168
EcoRII CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
EcoRV GATATC 1 cut(s) 216
EcoT14I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2802, 3043
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 1623, 2973
ErhI CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2802, 3043
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 803, 835, 1381, 1413
FaqI GGGAC 3 cut(s) 253, 1569, 2596
FauI CCCGC 1 cut(s) 500
FauNDI CATATG 1 cut(s) 1527
Fnu4HI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
FriOI GRGCYC 2 cut(s) 1623, 2973
Fsp4HI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
FspBI CTAG 5 cut(s) 32, 351, 611, 1901, 2823
FspI TGCGCA 1 cut(s) 2466
GlaI GCGC 2 cut(s) 1757, 2466
GluI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
GsuI CTGGAG 4 cut(s) 648, 862, 1800, 1892
HaeII RGCGCY 1 cut(s) 1759
HaeIII GGCC 7 cut(s) 286, 1150, 1156, 1582, 2942, 2971, 3148
HapII CCGG 5 cut(s) 245, 725, 1494, 2715, 2812
HgaI GACGC 2 cut(s) 250, 2500
HhaI GCGC 2 cut(s) 1758, 2467
Hin1I GRCGYC 2 cut(s) 242, 2492
Hin6I GCGC 2 cut(s) 1756, 2465
HinP1I GCGC 2 cut(s) 1756, 2465
HincII GTYRAC 1 cut(s) 2193
HindII GTYRAC 1 cut(s) 2193
HpaII CCGG 5 cut(s) 245, 725, 1494, 2715, 2812
Hpy166II GTNNAC 5 cut(s) 189, 1950, 2193, 2890, 2901
Hpy8I GTNNAC 5 cut(s) 189, 1950, 2193, 2890, 2901
Hpy99I CGWCG 3 cut(s) 66, 1810, 2280
HpyCH4IV ACGT 3 cut(s) 433, 781, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
HpyF3I CTNAG 8 cut(s) 137, 162, 192, 1302, 1559, 1617, 2917, 3170
HpySE526I ACGT 3 cut(s) 433, 781, 1432
Hsp92I GRCGYC 2 cut(s) 242, 2492
HspAI GCGC 2 cut(s) 1756, 2465
Kpn2I TCCGGA 1 cut(s) 2811
KpnI GGTACC 2 cut(s) 480, 2640
Kzo9I GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
LmnI GCTCC 4 cut(s) 715, 843, 2030, 3127
Lsp1109I GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
LweI GCATC 4 cut(s) 53, 544, 1026, 3015
MaeI CTAG 5 cut(s) 32, 351, 611, 1901, 2823
MaeII ACGT 3 cut(s) 433, 781, 1432
MaeIII GTNAC 6 cut(s) 199, 247, 259, 594, 874, 1285
MalI GATC 9 cut(s) 984, 1005, 1195, 1393, 2257, 2685, 2799, 2816, 3201
MbiI CCGCTC 3 cut(s) 113, 520, 2649
MboI GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
MfeI CAATTG 2 cut(s) 786, 1550
MflI RGATCY 4 cut(s) 982, 1003, 2255, 3199
MhlI GDGCHC 6 cut(s) 21, 1623, 1674, 1952, 2538, 2973
MlyI GAGTC 4 cut(s) 406, 845, 2560, 2813
MmeI TCCRAC 2 cut(s) 143, 2794
Mph1103I ATGCAT 1 cut(s) 559
MroI TCCGGA 1 cut(s) 2811
MroXI GAANNNNTTC 2 cut(s) 435, 2557
MseI TTAA 9 cut(s) 456, 680, 1278, 1862, 2111, 2526, 2613, 3005, 3120
MslI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3186
MspA1I CMGCKG 4 cut(s) 1271, 1474, 1691, 2386
MspCI CTTAAG 1 cut(s) 1861
MspI CCGG 5 cut(s) 245, 725, 1494, 2715, 2812
MspR9I CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2716
MunI CAATTG 2 cut(s) 786, 1550
MvaI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
MwoI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
NciI CCSGG 1 cut(s) 2716
NcoI CCATGG 2 cut(s) 1539, 2313
NdeI CATATG 1 cut(s) 1527
NdeII GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
NheI GCTAGC 1 cut(s) 610
NlaIV GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2971
NmuCI GTSAC 4 cut(s) 247, 259, 594, 1285
NsbI TGCGCA 1 cut(s) 2466
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 4 cut(s) 409, 2066, 2288, 2714
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 2714
PaeR7I CTCGAG 1 cut(s) 2705
PaqCI CACCTGC 1 cut(s) 1530
PasI CCCWGGG 1 cut(s) 1046
PceI AGGCCT 1 cut(s) 3148
PdmI GAANNNNTTC 2 cut(s) 435, 2557
PfoI TCCNGGA 1 cut(s) 2172
PkrI GCNGC 7 cut(s) 112, 343, 447, 1343, 1483, 1739, 2385
Ple19I CGATCG 1 cut(s) 1196
PleI GAGTC 4 cut(s) 405, 845, 2560, 2813
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 4 cut(s) 405, 845, 2560, 2813
Ppu21I YACGTR 1 cut(s) 782
PpuMI RGGWCCY 1 cut(s) 2048
PshAI GACNNNNGTC 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 2613
Psp124BI GAGCTC 1 cut(s) 1623
Psp5II RGGWCCY 1 cut(s) 2048
Psp6I CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
PspCI CACGTG 1 cut(s) 782
PspGI CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
PspN4I GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2971
PspOMI GGGCCC 1 cut(s) 2969
PspPPI RGGWCCY 1 cut(s) 2048
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 4 cut(s) 982, 1003, 2255, 3199
PvuI CGATCG 1 cut(s) 1196
PvuII CAGCTG 2 cut(s) 1271, 2386
RseI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3186
SacI GAGCTC 1 cut(s) 1623
SaqAI TTAA 9 cut(s) 456, 680, 1278, 1862, 2111, 2526, 2613, 3005, 3120
SatI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
Sau3AI GATC 9 cut(s) 982, 1003, 1193, 1391, 2255, 2683, 2797, 2814, 3199
SchI GAGTC 4 cut(s) 406, 845, 2560, 2813
ScrFI CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2716
SduI GDGCHC 6 cut(s) 21, 1623, 1674, 1952, 2538, 2973
SfaNI GCATC 4 cut(s) 53, 544, 1026, 3015
SfcI CTRYAG 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 2705
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3135
SlaI CTCGAG 1 cut(s) 2705
SmiMI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3186
SmlI CTYRAG 5 cut(s) 461, 515, 1861, 2705, 2881
SmoI CTYRAG 5 cut(s) 461, 515, 1861, 2705, 2881
SpeI ACTAGT 1 cut(s) 31
SphI GCATGC 1 cut(s) 2714
SseBI AGGCCT 1 cut(s) 3148
SsiI CCGC 9 cut(s) 111, 507, 520, 1474, 1689, 1738, 1762, 1919, 2647
SspMI CTAG 5 cut(s) 32, 351, 611, 1901, 2823
SstI GAGCTC 1 cut(s) 1623
StuI AGGCCT 1 cut(s) 3148
StyD4I CCNGG 6 cut(s) 154, 1045, 1150, 2172, 2537, 2714
StyI CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2802, 3043
TaiI ACGT 3 cut(s) 436, 784, 1435
TaqI TCGA 6 cut(s) 1112, 1192, 1980, 2551, 2706, 2817
TatI WGTACW 3 cut(s) 188, 2409, 2887
TauI GCSGC 2 cut(s) 113, 1740
Tru1I TTAA 9 cut(s) 456, 680, 1278, 1862, 2111, 2526, 2613, 3005, 3120
Tru9I TTAA 9 cut(s) 456, 680, 1278, 1862, 2111, 2526, 2613, 3005, 3120
TseFI GTSAC 4 cut(s) 247, 259, 594, 1285
TseI GCWGC 5 cut(s) 341, 445, 1341, 1481, 2383
Tsp45I GTSAC 4 cut(s) 247, 259, 594, 1285
Vha464I CTTAAG 1 cut(s) 1861
VneI GTGCAC 1 cut(s) 1948
VpaK11BI GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3135
VspI ATTAAT 1 cut(s) 2613
XapI RAATTY 6 cut(s) 53, 424, 547, 2168, 2238, 3001
XceI RCATGY 4 cut(s) 409, 2066, 2288, 2714
XcmI CCANNNNNNNNNTGG 2 cut(s) 1370, 3107
XhoI CTCGAG 1 cut(s) 2705
XmnI GAANNNNTTC 2 cut(s) 435, 2557
XspI CTAG 5 cut(s) 32, 351, 611, 1901, 2823
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.