Rroxscaffold_3G00245680

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005660
Physical Location & Seq
Reverse (-)
38742208 .. 38756597
14390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245680.1

Sequence Viewer

Length: 3195 bp
ATGGGAGAAGCACCAGTGCTAACTGCCACACTAGTAGGTGAAGCATCCTCCAAATTCACCCGATTTCCAAGTTCCACTTCCATTTTTGATACCAACTCTTACAGTGCCGCTCATCCAACCCCGATTTCGTTGCTGAGGTTGCACAAAAAGCCTGCTTCTCAGTCTGCCTTCATCAGAAAGAAGTGTACTAAGTTGGTTGCGAAAAGTTTGATATCCAAAATGGCTGGTGATGAAGGGACGCCAGCCACTGCAGATGTCACTTCAGGAGATATGATTTTCGCCCCTATTATTGAGGATGGTGTGTTCCGATTTGATTGTTCTGCAAATGATAGAGAAGCAGCACATCCTAGTCTCTCTTTTGTTAATGGCAAGGATAGGGAGACACCGATTCAGAGTGAAAAGATGCCGTCCTATATCCCAAATTTTGAACGTCTTCGTGGCCAGCAGGTTGTTAAACTTGAGCTTCCTGTTGGTACCTCTTTATACGGCACTGGGGAGGTTAGCGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAATTCGGATGCATGGGGCTATGGTTCTGGAACTACGTCATTGTACCAGTCACATCCTTGGGTGCTAGCTGTTCTTCCTTCTGGAGAGGCATTAGGAGTTCTTGCTGATACAACAAAGCGTTGTGAGATTGATTTAAGAAAAGAATCTTTGATGCGGTTCATCGCTCCATCCTCTTATCCTGTCATTACTTTTGGTCCATTTGCTACACCCAATGCTGTTTTGAGAGCTTTATCACATGCAATTGGTACTGTATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAATGTCGCTGGAGCTACGATTCTGATAAGAGAGTTCAAGAGGTTACAAGAACTTTTCGGGTGAAAAAGATTCCTTGTGACGTTGTATGGATGGATATTGATTATATGGATGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAAGGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAAGTGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTCTGGATTTCGAAAGCAGATGGAAAACCTTTTGTTGGGGAGGTGTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAGGCAAAAGTTCGATCGTGGTGGTCCAATTTAGTCAAAGATTTCATTTGTAATGGTGTTGATGGTATATGGAATGACATGAATGAACCAGCAGTTTTTAAGGTTGTCACAAAAACAATGCCTGAGAGCAATATGCATAAGGGGGATGATGAACTGGGAGGCTGCCAGAATCACTCACACTACCACAATGTCTATGGGTTGCTGATGGCAAGATCAACTTATGAAGGGATGAAATTGGGTAATGCAAAGAAACGTCCTTTTGTCCTCACAAGAGCTGGATTTATTGGTAGCCAGCGGTATGCTGCAACATGGACTGGAGACAATCTTTCGACTTGGGAACACCTGCATATGAGTATTCCCATGGTACTTCAATTGGGACTTAGTGGTCAGCCACTGTCGGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTCTTTGGAAGATGGATGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAAAAGGACACCGCTGACCATGAGCCATGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACCGCCTTATACCTCATTTATACACACTCTTCTATTTGGCTCATACGACGGGTACTCCAGTGGCAAGTCCTACATTTTTTGCTGATCCCAAAGATACCAGCTTAAGGAAATTGGAGACTTCTTTTTTGTTGGGTCCAGTTCTAGTGTATGCAAGCACTGCGGTTGGTCGGGGGATGGATGCTTTGCAGTGCACATTGCCCAAAGGAATCTGGATGAGTTTCGATTTTGATGATTCACATCCAGATTTACCAGCATTATATTTGCAAGGTGGAGCAATTATTCCTGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTAATATATTAGATGACTTGACACTCCTTGTGGCTTTAAATGAACATGGGAAAGCTGAAGGTGTTTTATATGAAGATGATGGTGATGGATATGAATTCCTGGAAGGAGGATTCTTGTTGACACACTATGTTGCTGAACTTCAATCATCAACATTTACTCTCAAAATTTCAAAAACTGAAGGATCTTGGAAGAGGCCAAACCGTCGCCTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATTTGGGGTAAGGATGGAGAGATTCTTCAACTTTCAATGCCTTCAGAACAGGAGGTCTCACAGCTGGTTTCTACCAGTGAGAAACAGTACAGAACTCGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGCGAGTTCTGCGCAGAAAGGAATAGAGCTTTCAAGGACGCCTATTGAATTGAAAGGTGGTGATTGGCTTATTAAAGTAGTGCCCTGGATTGGTGGTCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACCGAGTACCGCTCTGCTGGATGTTCCGAGGAATACAATGTTATTGAACGAAATCTCGAGCATGCCGGGGAGGAGGAATCTCTCATGTTAGAAGGTGATATAGGCGGTGGATTGGTTCTCCAACGACAGTTATATATTCCAAAGAATGATCCCAAGGTTTTGCAGATCGACTCTAGCATTATAGCACGCAAAGTTGGCGCTGGTTCTGGTGGATATTCAAGACTGGTCTGCTTGAGAGTACACCCGATGTTCACCCTGTTGCACCCCTCAGAATGTTATGTTTCATTTACGGCCGTTGACGGGTCAAAGCATGAAATTGGGCCCGAATCTAATGAGCAGTTCTATGAAGGGAATTTAATGCCTAATGGTGAATGGATGCTCATTGATAAATGCCTTGGCTTGGGGTTATTGAATCGGTTTGATGTCAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATTTGGAGCTGTGGTCCGAGGAGAGGCCTGTTTCAAACCAATCACCTCTTAGAATATCCCATGAGTATGAGGTGATAGATCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1064

Amino Acids

118.4

Weight (kDa)

5.84

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 150 - 225 3.3e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 266 - 595 1.4e-125 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 697 - 765 2.6e-12 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1527
AasI GACNNNNNNGTC 1 cut(s) 1560
Acc16I TGCGCA 1 cut(s) 2463
Acc36I ACCTGC 2 cut(s) 436, 1527
Acc65I GGTACC 2 cut(s) 473, 2633
AccB1I GGYRCC 3 cut(s) 473, 2306, 2633
AccBSI CCGCTC 3 cut(s) 110, 517, 2646
AclWI GGATC 5 cut(s) 974, 1008, 1835, 2260, 2777
AcoI YGGCCR 2 cut(s) 439, 2925
AcsI RAATTY 6 cut(s) 53, 421, 544, 2165, 2235, 2986
AcuI CTGAAG 5 cut(s) 246, 2148, 2268, 2346, 2559
AcyI GRCGYC 2 cut(s) 239, 2489
AfiI CCNNNNNNNGG 9 cut(s) 810, 1133, 1574, 1586, 1860, 2540, 2934, 3034, 3129
AflII CTTAAG 1 cut(s) 1858
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 4 cut(s) 1042, 1147, 2169, 2534
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 109, 141
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
Alw21I GWGCWC 2 cut(s) 1620, 1949
Alw26I GTCTC 5 cut(s) 356, 374, 1488, 1865, 2380
Alw44I GTGCAC 1 cut(s) 1945
AlwI GGATC 5 cut(s) 974, 1008, 1835, 2260, 2777
AlwNI CAGNNNCTG 2 cut(s) 248, 1570
Ama87I CYCGRG 1 cut(s) 2690
AoxI GGCC 8 cut(s) 439, 1145, 1151, 1577, 2264, 2925, 2954, 3131
ApaI GGGCCC 1 cut(s) 2958
ApaLI GTGCAC 1 cut(s) 1945
ApeKI GCWGC 3 cut(s) 338, 1338, 1478
ApoI RAATTY 6 cut(s) 53, 421, 544, 2165, 2235, 2986
ArsI GACNNNNNNTTYG 2 cut(s) 1864, 1896
AseI ATTAAT 1 cut(s) 2610
Asp700I GAANNNNTTC 2 cut(s) 432, 2554
Asp718I GGTACC 2 cut(s) 473, 2633
AspLEI GCGC 3 cut(s) 1755, 2464, 2834
AsuC2I CCSGG 1 cut(s) 2701
AsuII TTCGAA 1 cut(s) 1109
AsuNHI GCTAGC 1 cut(s) 607
AvaI CYCGRG 1 cut(s) 2690
AvaII GGWCC 7 cut(s) 737, 813, 967, 1200, 1889, 2045, 3120
BaeGI GKGCMC 4 cut(s) 1671, 1949, 2535, 2958
BaeI ACNNNNGTAYC 2 cut(s) 1800, 1833
BalI TGGCCA 1 cut(s) 441
BanI GGYRCC 3 cut(s) 473, 2306, 2633
BanII GRGCYC 2 cut(s) 1620, 2958
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 2 cut(s) 1620, 1949
BbvCI CCTCAGC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 350, 1325, 1465
BceAI ACGGC 5 cut(s) 391, 502, 1647, 2912, 2940
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 4 cut(s) 1044, 1149, 2171, 2536
BcnI CCSGG 1 cut(s) 2701
BcoDI GTCTC 5 cut(s) 356, 374, 1488, 1865, 2380
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 5 cut(s) 32, 348, 608, 1898, 2808
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 2 cut(s) 1756, 2835
BfrI CTTAAG 1 cut(s) 1858
BfuAI ACCTGC 2 cut(s) 436, 1527
BglII AGATCT 1 cut(s) 3184
BisI GCNGC 5 cut(s) 108, 339, 1339, 1479, 1735
BlsI GCNGC 5 cut(s) 109, 340, 1340, 1480, 1736
Bme1390I CCNGG 5 cut(s) 1044, 1149, 2171, 2536, 2701
Bme18I GGWCC 7 cut(s) 737, 813, 967, 1200, 1889, 2045, 3120
BmeT110I CYCGRG 1 cut(s) 2690
BmiI GGNNCC 7 cut(s) 475, 1578, 1890, 2046, 2308, 2635, 2956
BmrFI CCNGG 5 cut(s) 1044, 1149, 2171, 2536, 2701
BmrI ACTGGG 3 cut(s) 501, 1340, 3097
BmsI GCATC 7 cut(s) 53, 393, 541, 684, 1023, 1924, 3000
BmtI GCTAGC 1 cut(s) 611
BmuI ACTGGG 3 cut(s) 501, 1340, 3097
BpiI GAAGAC 1 cut(s) 425
BplI GAGNNNNNCTC 2 cut(s) 2630, 2662
BpmI CTGGAG 4 cut(s) 645, 859, 1512, 1797
Bpu10I CCTNAGC 2 cut(s) 134, 1614
Bpu14I TTCGAA 1 cut(s) 1109
BpuEI CTTGAG 3 cut(s) 479, 533, 2887
BpuMI CCSGG 1 cut(s) 2701
BsaBI GATNNNNATC 1 cut(s) 1992
BsaHI GRCGYC 2 cut(s) 239, 2489
BsaI GGTCTC 1 cut(s) 2380
BsaXI ACNNNNNCTCC 4 cut(s) 1795, 1825, 3104, 3134
Bsc4I CCNNNNNNNGG 9 cut(s) 810, 1133, 1574, 1586, 1860, 2540, 2934, 3034, 3129
Bse3DI GCAATG 1 cut(s) 1949
Bse8I GATNNNNATC 1 cut(s) 1992
BseBI CCWGG 4 cut(s) 1044, 1149, 2171, 2536
BseJI GATNNNNATC 1 cut(s) 1992
BseLI CCNNNNNNNGG 9 cut(s) 810, 1133, 1574, 1586, 1860, 2540, 2934, 3034, 3129
BseMI GCAATG 1 cut(s) 1949
BseMII CTCAG 5 cut(s) 125, 173, 1290, 1605, 2916
BseRI GAGGAG 4 cut(s) 1762, 2040, 2720, 3140
BseSI GKGCMC 4 cut(s) 1671, 1949, 2535, 2958
BseX3I CGGCCG 1 cut(s) 2925
BseXI GCAGC 3 cut(s) 350, 1325, 1465
Bsh1285I CGRYCG 2 cut(s) 1193, 2928
BshFI GGCC 8 cut(s) 441, 1147, 1153, 1579, 2266, 2927, 2956, 3133
BshNI GGYRCC 3 cut(s) 473, 2306, 2633
BsiEI CGRYCG 2 cut(s) 1193, 2928
BsiHKAI GWGCWC 2 cut(s) 1620, 1949
BsiHKCI CYCGRG 1 cut(s) 2690
BsiSI CCGG 1 cut(s) 2700
BslFI GGGAC 3 cut(s) 250, 1566, 2593
BslI CCNNNNNNNGG 9 cut(s) 810, 1133, 1574, 1586, 1860, 2540, 2934, 3034, 3129
BsmAI GTCTC 5 cut(s) 356, 374, 1488, 1865, 2380
BsmFI GGGAC 3 cut(s) 250, 1566, 2593
BsnI GGCC 8 cut(s) 441, 1147, 1153, 1579, 2266, 2927, 2956, 3133
Bso31I GGTCTC 1 cut(s) 2380
BsoBI CYCGRG 1 cut(s) 2690
Bsp119I TTCGAA 1 cut(s) 1109
Bsp120I GGGCCC 1 cut(s) 2954
Bsp1286I GDGCHC 5 cut(s) 1620, 1671, 1949, 2535, 2958
Bsp143I GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
Bsp19I CCATGG 3 cut(s) 1536, 1700, 2310
BspANI GGCC 8 cut(s) 441, 1147, 1153, 1579, 2266, 2927, 2956, 3133
BspCNI CTCAG 5 cut(s) 126, 172, 1291, 1606, 2915
BspLI GGNNCC 7 cut(s) 475, 1578, 1890, 2046, 2308, 2635, 2956
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 2 cut(s) 436, 1527
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 5 cut(s) 974, 1008, 1835, 2260, 2777
BspT104I TTCGAA 1 cut(s) 1109
BspT107I GGYRCC 3 cut(s) 473, 2306, 2633
BspTI CTTAAG 1 cut(s) 1858
BspTNI GGTCTC 1 cut(s) 2380
BsrBI CCGCTC 3 cut(s) 110, 517, 2646
BsrDI GCAATG 1 cut(s) 1949
BssMI GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
BssNI GRCGYC 2 cut(s) 239, 2489
BssT1I CCWWGG 8 cut(s) 599, 816, 990, 1536, 1700, 2310, 2787, 3028
Bst2UI CCWGG 4 cut(s) 1044, 1149, 2171, 2536
Bst6I CTCTTC 3 cut(s) 1710, 1790, 2255
BstACI GRCGYC 2 cut(s) 239, 2489
BstAFI CTTAAG 1 cut(s) 1858
BstAPI GCANNNNNTGC 2 cut(s) 1913, 2293
BstBI TTCGAA 1 cut(s) 1109
BstDEI CTNAG 8 cut(s) 134, 159, 189, 1299, 1556, 1614, 2902, 3155
BstDSI CCRYGG 4 cut(s) 1536, 1663, 1700, 2310
BstH2I RGCGCY 2 cut(s) 1756, 2835
BstHHI GCGC 3 cut(s) 1755, 2464, 2834
BstKTI GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
BstMAI GTCTC 5 cut(s) 356, 374, 1488, 1865, 2380
BstMBI GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
BstMCI CGRYCG 2 cut(s) 1193, 2928
BstMWI GCNNNNNNNGC 9 cut(s) 139, 148, 514, 1604, 1913, 2293, 2450, 2459, 2829
BstNI CCWGG 4 cut(s) 1044, 1149, 2171, 2536
BstNSI RCATGY 4 cut(s) 782, 2063, 2285, 2699
BstSCI CCNGG 5 cut(s) 1042, 1147, 2169, 2534, 2699
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 4 cut(s) 1671, 1949, 2535, 2958
BstV1I GCAGC 3 cut(s) 350, 1325, 1465
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 4 cut(s) 979, 1000, 2252, 3184
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 4 cut(s) 979, 1000, 2252, 3184
BstZI CGGCCG 1 cut(s) 2925
BsuRI GGCC 8 cut(s) 441, 1147, 1153, 1579, 2266, 2927, 2956, 3133
BtgI CCRYGG 4 cut(s) 1536, 1663, 1700, 2310
BtgZI GCGATG 1 cut(s) 688
BtsI GCAGTG 3 cut(s) 246, 1911, 1949
BveI ACCTGC 2 cut(s) 436, 1527
CaiI CAGNNNCTG 2 cut(s) 248, 1570
CfoI GCGC 3 cut(s) 1755, 2464, 2834
CseI GACGC 2 cut(s) 247, 2497
DdeI CTNAG 8 cut(s) 134, 159, 189, 1299, 1556, 1614, 2902, 3155
DpnI GATC 9 cut(s) 981, 1002, 1192, 1390, 1842, 2254, 2784, 2801, 3186
DpnII GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
DraI TTTAAA 1 cut(s) 2109
DrdI GACNNNNNNGTC 1 cut(s) 1560
DseDI GACNNNNNNGTC 1 cut(s) 1560
EaeI YGGCCR 2 cut(s) 439, 2925
EagI CGGCCG 1 cut(s) 2925
Eam1104I CTCTTC 3 cut(s) 1710, 1790, 2255
EarI CTCTTC 3 cut(s) 1710, 1790, 2255
Ecl136II GAGCTC 1 cut(s) 1618
EclXI CGGCCG 1 cut(s) 2925
Eco130I CCWWGG 8 cut(s) 599, 816, 990, 1536, 1700, 2310, 2787, 3028
Eco147I AGGCCT 1 cut(s) 3133
Eco24I GRGCYC 2 cut(s) 1620, 2958
Eco31I GGTCTC 1 cut(s) 2380
Eco32I GATATC 1 cut(s) 213
Eco47I GGWCC 7 cut(s) 737, 813, 967, 1200, 1889, 2045, 3120
Eco52I CGGCCG 1 cut(s) 2925
Eco53kI GAGCTC 1 cut(s) 1618
Eco57I CTGAAG 5 cut(s) 246, 2148, 2268, 2346, 2559
Eco88I CYCGRG 1 cut(s) 2690
EcoICRI GAGCTC 1 cut(s) 1618
EcoO109I RGGNCCY 2 cut(s) 1577, 2045
EcoRI GAATTC 2 cut(s) 544, 2165
EcoRII CCWGG 4 cut(s) 1042, 1147, 2169, 2534
EcoRV GATATC 1 cut(s) 213
EcoT14I CCWWGG 8 cut(s) 599, 816, 990, 1536, 1700, 2310, 2787, 3028
EcoT22I ATGCAT 2 cut(s) 556, 1314
EcoT38I GRGCYC 2 cut(s) 1620, 2958
ErhI CCWWGG 8 cut(s) 599, 816, 990, 1536, 1700, 2310, 2787, 3028
FalI AAGNNNNNCTT 6 cut(s) 61, 93, 800, 832, 1378, 1410
FaqI GGGAC 3 cut(s) 250, 1566, 2593
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 1 cut(s) 1524
Fnu4HI GCNGC 5 cut(s) 108, 339, 1339, 1479, 1735
FriOI GRGCYC 2 cut(s) 1620, 2958
Fsp4HI GCNGC 5 cut(s) 108, 339, 1339, 1479, 1735
FspBI CTAG 5 cut(s) 32, 348, 608, 1898, 2808
FspI TGCGCA 1 cut(s) 2463
GlaI GCGC 3 cut(s) 1754, 2463, 2833
GluI GCNGC 5 cut(s) 108, 339, 1339, 1479, 1735
GsuI CTGGAG 4 cut(s) 645, 859, 1512, 1797
HaeII RGCGCY 2 cut(s) 1756, 2835
HaeIII GGCC 8 cut(s) 441, 1147, 1153, 1579, 2266, 2927, 2956, 3133
HapII CCGG 1 cut(s) 2700
HgaI GACGC 2 cut(s) 247, 2497
HhaI GCGC 3 cut(s) 1755, 2464, 2834
Hin1I GRCGYC 2 cut(s) 239, 2489
Hin6I GCGC 3 cut(s) 1753, 2462, 2832
HinP1I GCGC 3 cut(s) 1753, 2462, 2832
HincII GTYRAC 2 cut(s) 2190, 2932
HindII GTYRAC 2 cut(s) 2190, 2932
HpaII CCGG 1 cut(s) 2700
Hpy166II GTNNAC 6 cut(s) 186, 1947, 2190, 2875, 2886, 2932
Hpy8I GTNNAC 6 cut(s) 186, 1947, 2190, 2875, 2886, 2932
Hpy99I CGWCG 2 cut(s) 1807, 2277
HpyCH4IV ACGT 4 cut(s) 430, 578, 909, 1429
HpyF10VI GCNNNNNNNGC 9 cut(s) 139, 148, 514, 1604, 1913, 2293, 2450, 2459, 2829
HpyF3I CTNAG 8 cut(s) 134, 159, 189, 1299, 1556, 1614, 2902, 3155
HpySE526I ACGT 4 cut(s) 430, 578, 909, 1429
Hsp92I GRCGYC 2 cut(s) 239, 2489
HspAI GCGC 3 cut(s) 1753, 2462, 2832
KpnI GGTACC 2 cut(s) 477, 2637
Kzo9I GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
LmnI GCTCC 4 cut(s) 712, 840, 2027, 3112
Lsp1109I GCAGC 3 cut(s) 350, 1325, 1465
LweI GCATC 7 cut(s) 53, 393, 541, 684, 1023, 1924, 3000
MaeI CTAG 5 cut(s) 32, 348, 608, 1898, 2808
MaeII ACGT 4 cut(s) 430, 578, 909, 1429
MaeIII GTNAC 5 cut(s) 256, 591, 871, 905, 1282
MalI GATC 9 cut(s) 981, 1002, 1192, 1390, 1842, 2254, 2784, 2801, 3186
MbiI CCGCTC 3 cut(s) 110, 517, 2646
MboI GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
MfeI CAATTG 2 cut(s) 783, 1547
MflI RGATCY 4 cut(s) 979, 1000, 2252, 3184
MhlI GDGCHC 5 cut(s) 1620, 1671, 1949, 2535, 2958
MlsI TGGCCA 1 cut(s) 441
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 2 cut(s) 2557, 2798
MmeI TCCRAC 2 cut(s) 140, 2779
Mox20I TGGCCA 1 cut(s) 441
Mph1103I ATGCAT 2 cut(s) 556, 1314
MroXI GAANNNNTTC 2 cut(s) 432, 2554
MscI TGGCCA 1 cut(s) 441
MslI CAYNNNNRTG 5 cut(s) 1011, 2058, 2064, 2558, 3171
Msp20I TGGCCA 1 cut(s) 441
MspA1I CMGCKG 3 cut(s) 1471, 1688, 2383
MspCI CTTAAG 1 cut(s) 1858
MspI CCGG 1 cut(s) 2700
MspR9I CCNGG 5 cut(s) 1044, 1149, 2171, 2536, 2701
MunI CAATTG 2 cut(s) 783, 1547
MvaI CCWGG 4 cut(s) 1044, 1149, 2171, 2536
MwoI GCNNNNNNNGC 9 cut(s) 139, 148, 514, 1604, 1913, 2293, 2450, 2459, 2829
NciI CCSGG 1 cut(s) 2701
NcoI CCATGG 3 cut(s) 1536, 1700, 2310
NdeI CATATG 1 cut(s) 1524
NdeII GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
NheI GCTAGC 1 cut(s) 607
NlaIV GGNNCC 7 cut(s) 475, 1578, 1890, 2046, 2308, 2635, 2956
NmuCI GTSAC 4 cut(s) 256, 591, 905, 1282
NsbI TGCGCA 1 cut(s) 2463
NsiI ATGCAT 2 cut(s) 556, 1314
NspI RCATGY 4 cut(s) 782, 2063, 2285, 2699
NspV TTCGAA 1 cut(s) 1109
PaeI GCATGC 1 cut(s) 2699
PaeR7I CTCGAG 1 cut(s) 2690
PaqCI CACCTGC 1 cut(s) 1527
PasI CCCWGGG 1 cut(s) 1043
PceI AGGCCT 1 cut(s) 3133
PdmI GAANNNNTTC 2 cut(s) 432, 2554
PfoI TCCNGGA 1 cut(s) 2169
PkrI GCNGC 5 cut(s) 109, 340, 1340, 1480, 1736
Ple19I CGATCG 1 cut(s) 1193
PleI GAGTC 2 cut(s) 2557, 2798
PpsI GAGTC 2 cut(s) 2557, 2798
PpuMI RGGWCCY 1 cut(s) 2045
PshBI ATTAAT 1 cut(s) 2610
Psp124BI GAGCTC 1 cut(s) 1620
Psp5II RGGWCCY 1 cut(s) 2045
Psp6I CCWGG 4 cut(s) 1042, 1147, 2169, 2534
PspGI CCWGG 4 cut(s) 1042, 1147, 2169, 2534
PspN4I GGNNCC 7 cut(s) 475, 1578, 1890, 2046, 2308, 2635, 2956
PspOMI GGGCCC 1 cut(s) 2954
PspPPI RGGWCCY 1 cut(s) 2045
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 2 cut(s) 248, 1570
PsuI RGATCY 4 cut(s) 979, 1000, 2252, 3184
PvuI CGATCG 1 cut(s) 1193
PvuII CAGCTG 1 cut(s) 2383
RseI CAYNNNNRTG 5 cut(s) 1011, 2058, 2064, 2558, 3171
SacI GAGCTC 1 cut(s) 1620
SatI GCNGC 5 cut(s) 108, 339, 1339, 1479, 1735
Sau3AI GATC 9 cut(s) 979, 1000, 1190, 1388, 1840, 2252, 2782, 2799, 3184
SchI GAGTC 2 cut(s) 2557, 2798
ScrFI CCNGG 5 cut(s) 1044, 1149, 2171, 2536, 2701
SduI GDGCHC 5 cut(s) 1620, 1671, 1949, 2535, 2958
SfaNI GCATC 7 cut(s) 53, 393, 541, 684, 1023, 1924, 3000
SfcI CTRYAG 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 2690
SfuI TTCGAA 1 cut(s) 1109
SinI GGWCC 7 cut(s) 737, 813, 967, 1200, 1889, 2045, 3120
SlaI CTCGAG 1 cut(s) 2690
SmiMI CAYNNNNRTG 5 cut(s) 1011, 2058, 2064, 2558, 3171
SmlI CTYRAG 5 cut(s) 458, 512, 1858, 2690, 2866
SmoI CTYRAG 5 cut(s) 458, 512, 1858, 2690, 2866
SpeI ACTAGT 1 cut(s) 31
SphI GCATGC 1 cut(s) 2699
SseBI AGGCCT 1 cut(s) 3133
SspMI CTAG 5 cut(s) 32, 348, 608, 1898, 2808
SstI GAGCTC 1 cut(s) 1620
StuI AGGCCT 1 cut(s) 3133
StyD4I CCNGG 5 cut(s) 1042, 1147, 2169, 2534, 2699
StyI CCWWGG 8 cut(s) 599, 816, 990, 1536, 1700, 2310, 2787, 3028
TaiI ACGT 4 cut(s) 433, 581, 912, 1432
TaqI TCGA 7 cut(s) 1109, 1189, 1505, 1977, 2548, 2691, 2802
TatI WGTACW 3 cut(s) 185, 2406, 2872
TauI GCSGC 2 cut(s) 110, 1737
TseFI GTSAC 4 cut(s) 256, 591, 905, 1282
TseI GCWGC 3 cut(s) 338, 1338, 1478
Tsp45I GTSAC 4 cut(s) 256, 591, 905, 1282
Vha464I CTTAAG 1 cut(s) 1858
VneI GTGCAC 1 cut(s) 1945
VpaK11BI GGWCC 7 cut(s) 737, 813, 967, 1200, 1889, 2045, 3120
VspI ATTAAT 1 cut(s) 2610
XapI RAATTY 6 cut(s) 53, 421, 544, 2165, 2235, 2986
XceI RCATGY 4 cut(s) 782, 2063, 2285, 2699
XcmI CCANNNNNNNNNTGG 2 cut(s) 1367, 3092
XhoI CTCGAG 1 cut(s) 2690
XmnI GAANNNNTTC 2 cut(s) 432, 2554
XspI CTAG 5 cut(s) 32, 348, 608, 1898, 2808
Zsp2I ATGCAT 2 cut(s) 556, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.