Rh7CG296000

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005660
Physical Location & Seq
Forward (+)
30344512 .. 30357267
12756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG296000.1

Sequence Viewer

Length: 3198 bp
ATGGGAGAAGCACCAGTGCTAACTGCCACATTAGTAGGTGAAGCATCCTCCAAATTCACCCGACGATTTCCAAGTACCACTTCCATTTTTGATACCAACTCTTACAGTGCCGCTCAACCAACCCCGATTTCGTTGCTGAGGTTGAACAAGAAGCCTGGTTCTCAGTCTGCCTTCATCAGAAAGAAGTGTACGAAGTTGGTTACGAAAAGTTTGATATCCAAAATGGCTGGTGATGAAGGGACGCCGGTCACTGCAGATGTCACTTCAGGAGATATGATTTTCGGGCCTATCATTGAGGATGGTGTGTTCCGATTTGATTGTTCTGCAAATGATAGAGAAGCAGCACATCCTAGTCTCTCTTTTGTTAATGGCAAGGATAGGGAGACACCGATTCAGAGTCAGAACATGCCGTCCTATATCCCAAATTTTGAATGTCTTCTTGGGCAGCAGATTGTTAAACTTGAGCTTCCTGTTGGTACCTCTTTATACGGCACTGGGGAGGTTAGCGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAATTCGGATGCATGGGGCTATGGTTCTGGAACTACGTCGTTGTACCAGTCACATCCTTGGGTGCTAGCTGTTCTTCCTTCTGGAGAGGCATTAGGAGTTCTTGCGGATACAACAAAGCGTTGTGAGATTGATTTAAGAAAAGAATCTTTGATACGGTTCATCGCTCCATCCTCTTATCCTGTCATTACTTTTGGTCCATTTGCTACACCCAATGCTGTTTTGAGAGCTTTATCACGTGCAATTGGTACTGTATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAATGTCGCTGGAGCTATGATTCTGATAAGAGAGTTCAAGAGGTTACAAGAACTTTTCGGGAGAAAAAGATTCCTTGTGATGTCGTATGGATGGATATTGATTATATGGATGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAAGGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAAGTGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTCTGGATTTCGAAAGCAGATGGAAAACCTTTTGTTGGGGAGGTGTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAGGCAAAAGTTCGATCGTGGTGGTCCAATTTAGTCAAAGATTTCATTTGTAATGGTGTTGATGGTATATGGAATGACATGAATGAACCAGCTGTTTTTAAGGTTGTCACAAAAACAATGCCTGAGAGCAATATCCATAAGGGGGATGATGAACTGGGAGGCTGCCAGAATCACTCACACTACCACAATGTCTATGGGTTGCTGATGGCAAGATCAACTTATGAAGGGATGAAATTGGGTAATGCAAAGAAACGTCCTTTTGTCCTCACGAGAGCTGGATTTATTGGTAGCCAGCGGTATGCTGCAACATGGACCGGAGACAATCTTTCAACTTGGGAACACCTGCATATGAGTATTCCCATGGTACTTCAATTGGGACTTAGTGGTCAGCCAATGTCGGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTCTTTGGAAGATGGGTGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAAAAGGACACCGCTGACCATGAGCCGTGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACCGCCTTATACCTCATTTATACACTCTCTTCTATATGGCTCATACGACGGGTACTCCAGTGGCAAGTCCTACATTTTTTGCTGATCCCAAAGATACCAGCTTAAGGAAATTGGAGACTTCTTTTCTGTTGGGTCCAGTTCTAGTGTATGCAAGCACTGCGGTTGGTCGGGGGATGGATGCTTTGCAGTGCACATTGCCCAAAGGAATTTGGATGAGTTTCGATTTTGATGATTCACATCCAGATTTACCAGCGTTATATTTGCAAGGTGGAGCAATTATTCCTGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTAATATATTAGATGACTTGACACTCCTTGTGGCTTTAAATGAACATGGGAAAGCTGAAGGTGTTTTATATGAAGATGATGGTGATGGATATGAATTCCTGGAAGGAGGATTCTTGTTGACACACTATGTTGCTGAACTTCAATCATCAACACTTACTCTCAAAATTTCAAAAACTGAAGGATCTTGGAAGAGGCCAAACCGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATATGGGGTAAGGATGGAGAGATTCTTCAACTTTCAGCGCCTTCAGAACAGGAGGTCTCGCAGCTGGTTTCTACCAGTGAGAAACAGTACAGAAGTCGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGTGAGTTCTGCGCAGAAAGGAATAGAGCTTTCAAGGACGCCTATTGAATTGAAAGGTGGTGATTGGGTTATTAAAGTAGTGCCCTGGATTGGTGGTCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACCGAGTACCGCTCTGCTGGATGTTCTGAGGAATACAATGTTATTGAACGAAATCCCGAGCATGCCGGGGAGGAGGAATCTCTCATGTTAGAAGGTGACATAGGGGGTGGATTGGTTCTCCAACGACAGTTCTATATTCCAAAGAATGATCCCAAGGTTTTCCGGATCGACTCTAGCATTATAGCACGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGGCTGGTCTGCTTGAGAGTACACCCGATGTTCACCCTGTTGCACCCCTCAGAATCTTATGTTTCATTTACAGCCATTGACGGGTCAAAGCATGAAATTGGGCCCGAATCTAACGAGCAGTTCTATGAAGGGAATTTAATGCCTAATGGTGAATGGATGCTTATTGATAAATGCCTTGGCTTGGGGTTATTGAATCGGTTTGAGGTCAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATTTGGAGCTGTGGTCCGAGGAGAGGCCTGTTTCAAACCAATCACCTCTTAGAATATCCCATGAGTATGAGGTGATAGATCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1065

Amino Acids

118.49

Weight (kDa)

5.76

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 152 - 226 8e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 267 - 596 1.9e-126 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 698 - 766 3.9e-13 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1530
AasI GACNNNNNNGTC 1 cut(s) 1563
Acc16I TGCGCA 1 cut(s) 2466
Acc36I ACCTGC 1 cut(s) 1530
Acc65I GGTACC 2 cut(s) 476, 2636
AccB1I GGYRCC 3 cut(s) 476, 2309, 2636
AccBSI CCGCTC 3 cut(s) 113, 520, 2649
AccIII TCCGGA 1 cut(s) 2799
AclWI GGATC 6 cut(s) 977, 1011, 1838, 2263, 2780, 2810
AcsI RAATTY 7 cut(s) 53, 424, 547, 1965, 2168, 2238, 2989
AcuI CTGAAG 5 cut(s) 249, 2151, 2271, 2349, 2562
AcvI CACGTG 1 cut(s) 782
AcyI GRCGYC 2 cut(s) 242, 2492
AflII CTTAAG 1 cut(s) 1861
AjnI CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
AjuI GAANNNNNNNTTGG 6 cut(s) 64, 96, 112, 144, 423, 455
AloI GAACNNNNNNTCC 4 cut(s) 290, 322, 395, 427
Alw21I GWGCWC 2 cut(s) 1623, 1952
Alw26I GTCTC 5 cut(s) 359, 377, 1491, 1868, 2383
Alw44I GTGCAC 1 cut(s) 1948
AlwI GGATC 6 cut(s) 977, 1011, 1838, 2263, 2780, 2810
Ama87I CYCGRG 1 cut(s) 2693
Aor13HI TCCGGA 1 cut(s) 2799
AoxI GGCC 7 cut(s) 284, 1148, 1154, 1580, 2267, 2957, 3134
ApaI GGGCCC 1 cut(s) 2961
ApaLI GTGCAC 1 cut(s) 1948
ApeKI GCWGC 5 cut(s) 341, 445, 1341, 1481, 2383
ApoI RAATTY 7 cut(s) 53, 424, 547, 1965, 2168, 2238, 2989
AseI ATTAAT 1 cut(s) 2613
Asp700I GAANNNNTTC 2 cut(s) 435, 2557
Asp718I GGTACC 2 cut(s) 476, 2636
AspLEI GCGC 3 cut(s) 1758, 2362, 2467
AsuC2I CCSGG 1 cut(s) 2704
AsuII TTCGAA 1 cut(s) 1112
AsuNHI GCTAGC 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 2693
AvaII GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3123
BaeGI GKGCMC 4 cut(s) 1674, 1952, 2538, 2961
BaeI ACNNNNGTAYC 2 cut(s) 1803, 1836
BanI GGYRCC 3 cut(s) 476, 2309, 2636
BanII GRGCYC 2 cut(s) 1623, 2961
BauI CACGAG 1 cut(s) 1447
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 1623, 1952
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
BceAI ACGGC 4 cut(s) 394, 505, 1650, 1687
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 1 cut(s) 2704
BcoDI GTCTC 5 cut(s) 359, 377, 1491, 1868, 2383
BfaI CTAG 4 cut(s) 351, 611, 1901, 2811
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 2 cut(s) 1759, 2363
BfrI CTTAAG 1 cut(s) 1861
BfuAI ACCTGC 1 cut(s) 1530
BfuI GTATCC 1 cut(s) 646
BglII AGATCT 1 cut(s) 3187
BisI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
BlsI GCNGC 7 cut(s) 112, 343, 447, 1343, 1483, 1739, 2385
Bme1390I CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2704
Bme18I GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3123
BmeT110I CYCGRG 1 cut(s) 2693
BmiI GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2959
BmrFI CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2704
BmrI ACTGGG 3 cut(s) 504, 1343, 3100
BmsI GCATC 5 cut(s) 53, 544, 1026, 1927, 3003
BmtI GCTAGC 1 cut(s) 614
BmuI ACTGGG 3 cut(s) 504, 1343, 3100
BoxI GACNNNNGTC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 2633, 2665
BpmI CTGGAG 3 cut(s) 648, 862, 1800
Bpu10I CCTNAGC 2 cut(s) 137, 1617
Bpu14I TTCGAA 1 cut(s) 1112
BpuEI CTTGAG 3 cut(s) 482, 536, 2890
BpuMI CCSGG 1 cut(s) 2704
BsaAI YACGTR 1 cut(s) 782
BsaBI GATNNNNATC 1 cut(s) 1995
BsaHI GRCGYC 2 cut(s) 242, 2492
BsaI GGTCTC 1 cut(s) 2383
BsaWI WCCGGW 2 cut(s) 1493, 2799
BsaXI ACNNNNNCTCC 4 cut(s) 1798, 1828, 3107, 3137
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 9 cut(s) 14, 499, 592, 1338, 1817, 1895, 2397, 3095, 3103
Bse3DI GCAATG 1 cut(s) 1952
Bse8I GATNNNNATC 1 cut(s) 1995
BseAI TCCGGA 1 cut(s) 2799
BseBI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BseJI GATNNNNATC 1 cut(s) 1995
BseMI GCAATG 1 cut(s) 1952
BseMII CTCAG 6 cut(s) 128, 176, 1293, 1608, 2655, 2919
BseNI ACTGG 9 cut(s) 14, 499, 592, 1338, 1817, 1895, 2397, 3095, 3103
BseRI GAGGAG 4 cut(s) 1765, 2043, 2723, 3143
BseSI GKGCMC 4 cut(s) 1674, 1952, 2538, 2961
BseXI GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2269, 2959, 3136
BshNI GGYRCC 3 cut(s) 476, 2309, 2636
BsiEI CGRYCG 1 cut(s) 1196
BsiHKAI GWGCWC 2 cut(s) 1623, 1952
BsiHKCI CYCGRG 1 cut(s) 2693
BsiSI CCGG 4 cut(s) 245, 1494, 2703, 2800
BslFI GGGAC 3 cut(s) 253, 1569, 2596
BsmAI GTCTC 5 cut(s) 359, 377, 1491, 1868, 2383
BsmFI GGGAC 3 cut(s) 253, 1569, 2596
BsnI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2269, 2959, 3136
Bso31I GGTCTC 1 cut(s) 2383
BsoBI CYCGRG 1 cut(s) 2693
Bsp119I TTCGAA 1 cut(s) 1112
Bsp120I GGGCCC 1 cut(s) 2957
Bsp1286I GDGCHC 5 cut(s) 1623, 1674, 1952, 2538, 2961
Bsp13I TCCGGA 1 cut(s) 2799
Bsp143I GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
Bsp19I CCATGG 2 cut(s) 1539, 2313
BspANI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2269, 2959, 3136
BspCNI CTCAG 6 cut(s) 129, 175, 1294, 1609, 2656, 2918
BspEI TCCGGA 1 cut(s) 2799
BspLI GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2959
BspMAI CTGCAG 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 1530
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 6 cut(s) 977, 1011, 1838, 2263, 2780, 2810
BspT104I TTCGAA 1 cut(s) 1112
BspT107I GGYRCC 3 cut(s) 476, 2309, 2636
BspTI CTTAAG 1 cut(s) 1861
BspTNI GGTCTC 1 cut(s) 2383
BsrBI CCGCTC 3 cut(s) 113, 520, 2649
BsrDI GCAATG 1 cut(s) 1952
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 9 cut(s) 14, 499, 592, 1338, 1817, 1895, 2397, 3095, 3103
BssAI RCCGGY 1 cut(s) 244
BssMI GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
BssNI GRCGYC 2 cut(s) 242, 2492
BssSI CACGAG 1 cut(s) 1447
BssT1I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2790, 3031
Bst2BI CACGAG 1 cut(s) 1447
Bst2UI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
Bst6I CTCTTC 3 cut(s) 1713, 1793, 2258
BstACI GRCGYC 2 cut(s) 242, 2492
BstAFI CTTAAG 1 cut(s) 1861
BstAPI GCANNNNNTGC 2 cut(s) 1916, 2296
BstBAI YACGTR 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 7 cut(s) 509, 612, 1472, 1912, 2282, 2700, 2824
BstDEI CTNAG 8 cut(s) 137, 162, 1302, 1559, 1617, 2664, 2905, 3158
BstDSI CCRYGG 4 cut(s) 1539, 1666, 1703, 2313
BstH2I RGCGCY 2 cut(s) 1759, 2363
BstHHI GCGC 3 cut(s) 1758, 2362, 2467
BstKTI GATC 9 cut(s) 985, 1006, 1196, 1394, 1846, 2258, 2788, 2805, 3190
BstMAI GTCTC 5 cut(s) 359, 377, 1491, 1868, 2383
BstMBI GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
BstNI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
BstNSI RCATGY 4 cut(s) 409, 2066, 2288, 2702
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 6 cut(s) 154, 1045, 1150, 2172, 2537, 2702
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 4 cut(s) 1674, 1952, 2538, 2961
BstV1I GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 4 cut(s) 982, 1003, 2255, 3187
BstXI CCANNNNNNTGG 1 cut(s) 1016
BstYI RGATCY 4 cut(s) 982, 1003, 2255, 3187
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 7 cut(s) 286, 1150, 1156, 1582, 2269, 2959, 3136
BtgI CCRYGG 4 cut(s) 1539, 1666, 1703, 2313
BtgZI GCGATG 1 cut(s) 691
BtsI GCAGTG 3 cut(s) 249, 1914, 1952
BveI ACCTGC 1 cut(s) 1530
Cac8I GCNNGC 7 cut(s) 509, 612, 1472, 1912, 2282, 2700, 2824
CfoI GCGC 3 cut(s) 1758, 2362, 2467
Cfr10I RCCGGY 1 cut(s) 244
CseI GACGC 2 cut(s) 250, 2500
DdeI CTNAG 8 cut(s) 137, 162, 1302, 1559, 1617, 2664, 2905, 3158
DpnI GATC 9 cut(s) 984, 1005, 1195, 1393, 1845, 2257, 2787, 2804, 3189
DpnII GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
DraI TTTAAA 1 cut(s) 2112
DrdI GACNNNNNNGTC 1 cut(s) 1563
DseDI GACNNNNNNGTC 1 cut(s) 1563
Eam1104I CTCTTC 3 cut(s) 1713, 1793, 2258
EarI CTCTTC 3 cut(s) 1713, 1793, 2258
Ecl136II GAGCTC 1 cut(s) 1621
Eco130I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2790, 3031
Eco147I AGGCCT 1 cut(s) 3136
Eco24I GRGCYC 2 cut(s) 1623, 2961
Eco31I GGTCTC 1 cut(s) 2383
Eco32I GATATC 1 cut(s) 216
Eco47I GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3123
Eco53kI GAGCTC 1 cut(s) 1621
Eco57I CTGAAG 5 cut(s) 249, 2151, 2271, 2349, 2562
Eco72I CACGTG 1 cut(s) 782
Eco88I CYCGRG 1 cut(s) 2693
EcoICRI GAGCTC 1 cut(s) 1621
EcoO109I RGGNCCY 2 cut(s) 1580, 2048
EcoRI GAATTC 2 cut(s) 547, 2168
EcoRII CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
EcoRV GATATC 1 cut(s) 216
EcoT14I CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2790, 3031
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 1623, 2961
ErhI CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2790, 3031
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 803, 835, 1381, 1413
FaqI GGGAC 3 cut(s) 253, 1569, 2596
FauI CCCGC 1 cut(s) 500
FauNDI CATATG 1 cut(s) 1527
Fnu4HI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
FriOI GRGCYC 2 cut(s) 1623, 2961
Fsp4HI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
FspBI CTAG 4 cut(s) 351, 611, 1901, 2811
FspI TGCGCA 1 cut(s) 2466
GlaI GCGC 3 cut(s) 1757, 2361, 2466
GluI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
GsuI CTGGAG 3 cut(s) 648, 862, 1800
HaeII RGCGCY 2 cut(s) 1759, 2363
HaeIII GGCC 7 cut(s) 286, 1150, 1156, 1582, 2269, 2959, 3136
HapII CCGG 4 cut(s) 245, 1494, 2703, 2800
HgaI GACGC 2 cut(s) 250, 2500
HhaI GCGC 3 cut(s) 1758, 2362, 2467
Hin1I GRCGYC 2 cut(s) 242, 2492
Hin6I GCGC 3 cut(s) 1756, 2360, 2465
HinP1I GCGC 3 cut(s) 1756, 2360, 2465
HincII GTYRAC 1 cut(s) 2193
HindII GTYRAC 1 cut(s) 2193
HpaII CCGG 4 cut(s) 245, 1494, 2703, 2800
Hpy166II GTNNAC 5 cut(s) 189, 1950, 2193, 2878, 2889
Hpy8I GTNNAC 5 cut(s) 189, 1950, 2193, 2878, 2889
Hpy99I CGWCG 4 cut(s) 66, 586, 1810, 2280
HpyCH4IV ACGT 3 cut(s) 581, 781, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
HpyF3I CTNAG 8 cut(s) 137, 162, 1302, 1559, 1617, 2664, 2905, 3158
HpySE526I ACGT 3 cut(s) 581, 781, 1432
Hsp92I GRCGYC 2 cut(s) 242, 2492
HspAI GCGC 3 cut(s) 1756, 2360, 2465
Kpn2I TCCGGA 1 cut(s) 2799
KpnI GGTACC 2 cut(s) 480, 2640
Kzo9I GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
LmnI GCTCC 4 cut(s) 715, 843, 2030, 3115
Lsp1109I GCAGC 5 cut(s) 353, 457, 1328, 1468, 2395
LweI GCATC 5 cut(s) 53, 544, 1026, 1927, 3003
MaeI CTAG 4 cut(s) 351, 611, 1901, 2811
MaeII ACGT 3 cut(s) 581, 781, 1432
MaeIII GTNAC 7 cut(s) 199, 247, 259, 594, 874, 1285, 2732
MalI GATC 9 cut(s) 984, 1005, 1195, 1393, 1845, 2257, 2787, 2804, 3189
MbiI CCGCTC 3 cut(s) 113, 520, 2649
MboI GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
MfeI CAATTG 2 cut(s) 786, 1550
MflI RGATCY 4 cut(s) 982, 1003, 2255, 3187
MhlI GDGCHC 5 cut(s) 1623, 1674, 1952, 2538, 2961
MlyI GAGTC 3 cut(s) 406, 2560, 2801
MmeI TCCRAC 1 cut(s) 2782
Mph1103I ATGCAT 1 cut(s) 559
MroI TCCGGA 1 cut(s) 2799
MroXI GAANNNNTTC 2 cut(s) 435, 2557
MslI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3174
MspA1I CMGCKG 4 cut(s) 1271, 1474, 1691, 2386
MspCI CTTAAG 1 cut(s) 1861
MspI CCGG 4 cut(s) 245, 1494, 2703, 2800
MspR9I CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2704
MunI CAATTG 2 cut(s) 786, 1550
MvaI CCWGG 5 cut(s) 156, 1047, 1152, 2174, 2539
MwoI GCNNNNNNNGC 4 cut(s) 517, 1607, 1916, 2296
NciI CCSGG 1 cut(s) 2704
NcoI CCATGG 2 cut(s) 1539, 2313
NdeI CATATG 1 cut(s) 1527
NdeII GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
NheI GCTAGC 1 cut(s) 610
NlaIV GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2959
NmuCI GTSAC 5 cut(s) 247, 259, 594, 1285, 2732
NsbI TGCGCA 1 cut(s) 2466
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 4 cut(s) 409, 2066, 2288, 2702
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 2702
PaqCI CACCTGC 1 cut(s) 1530
PasI CCCWGGG 1 cut(s) 1046
PceI AGGCCT 1 cut(s) 3136
PdmI GAANNNNTTC 2 cut(s) 435, 2557
PfoI TCCNGGA 1 cut(s) 2172
PkrI GCNGC 7 cut(s) 112, 343, 447, 1343, 1483, 1739, 2385
Ple19I CGATCG 1 cut(s) 1196
PleI GAGTC 3 cut(s) 405, 2560, 2801
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 3 cut(s) 405, 2560, 2801
Ppu21I YACGTR 1 cut(s) 782
PpuMI RGGWCCY 1 cut(s) 2048
PshAI GACNNNNGTC 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 2613
Psp124BI GAGCTC 1 cut(s) 1623
Psp5II RGGWCCY 1 cut(s) 2048
Psp6I CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
PspCI CACGTG 1 cut(s) 782
PspGI CCWGG 5 cut(s) 154, 1045, 1150, 2172, 2537
PspN4I GGNNCC 7 cut(s) 478, 1581, 1893, 2049, 2311, 2638, 2959
PspOMI GGGCCC 1 cut(s) 2957
PspPPI RGGWCCY 1 cut(s) 2048
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 4 cut(s) 982, 1003, 2255, 3187
PvuI CGATCG 1 cut(s) 1196
PvuII CAGCTG 2 cut(s) 1271, 2386
RseI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3174
SacI GAGCTC 1 cut(s) 1623
SatI GCNGC 7 cut(s) 111, 342, 446, 1342, 1482, 1738, 2384
Sau3AI GATC 9 cut(s) 982, 1003, 1193, 1391, 1843, 2255, 2785, 2802, 3187
SchI GAGTC 3 cut(s) 406, 2560, 2801
ScrFI CCNGG 6 cut(s) 156, 1047, 1152, 2174, 2539, 2704
SduI GDGCHC 5 cut(s) 1623, 1674, 1952, 2538, 2961
SfaNI GCATC 5 cut(s) 53, 544, 1026, 1927, 3003
SfcI CTRYAG 1 cut(s) 252
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3123
SmiMI CAYNNNNRTG 5 cut(s) 1014, 2061, 2067, 2561, 3174
SmlI CTYRAG 4 cut(s) 461, 515, 1861, 2869
SmoI CTYRAG 4 cut(s) 461, 515, 1861, 2869
SphI GCATGC 1 cut(s) 2702
SseBI AGGCCT 1 cut(s) 3136
SspMI CTAG 4 cut(s) 351, 611, 1901, 2811
SstI GAGCTC 1 cut(s) 1623
StuI AGGCCT 1 cut(s) 3136
StyD4I CCNGG 6 cut(s) 154, 1045, 1150, 2172, 2537, 2702
StyI CCWWGG 7 cut(s) 602, 819, 993, 1539, 2313, 2790, 3031
TaiI ACGT 3 cut(s) 584, 784, 1435
TaqI TCGA 5 cut(s) 1112, 1192, 1980, 2551, 2805
TatI WGTACW 2 cut(s) 2409, 2875
TauI GCSGC 2 cut(s) 113, 1740
TseFI GTSAC 5 cut(s) 247, 259, 594, 1285, 2732
TseI GCWGC 5 cut(s) 341, 445, 1341, 1481, 2383
Tsp45I GTSAC 5 cut(s) 247, 259, 594, 1285, 2732
Vha464I CTTAAG 1 cut(s) 1861
VneI GTGCAC 1 cut(s) 1948
VpaK11BI GGWCC 8 cut(s) 740, 816, 970, 1203, 1491, 1892, 2048, 3123
VspI ATTAAT 1 cut(s) 2613
XapI RAATTY 7 cut(s) 53, 424, 547, 1965, 2168, 2238, 2989
XceI RCATGY 4 cut(s) 409, 2066, 2288, 2702
XcmI CCANNNNNNNNNTGG 2 cut(s) 1370, 3095
XmnI GAANNNNTTC 2 cut(s) 435, 2557
XspI CTAG 4 cut(s) 351, 611, 1901, 2811
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.