FvH4_5g24150

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005660
Physical Location & Seq
Forward (+)
15426486 .. 15438744
12259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24150.t3

Sequence Viewer

Length: 3192 bp
ATGGGAGAAGTACCAGTCCTCACTCGCACACTACTATTTGGACGATCCTCCAGTTTCACACTCACAACTAACCGTCTTCATCTTCATCGCACTTCCATTTCTGATACCAACACTCCCAGTGCCCCCCATGCAACTCCGATTTCGTTGCTCAGGTTGAAGAAGAAGAAGACGAAGCCTCCTTCTCCGTTCGCCTTCATCAGAAATAAGTCTACAAATTTGGTTACTAGAAGTTTGAGATCGAAAATGGTTGGTGATGAAGGGACGCCAGTCAGTGCAGATGTCACTTCAGGAGAGATGATTTTCGAGCCTATTATCGAGGATGGTGTGTTTCGATTTGATTGTTCTGGAAATGATAGAGAAGCGGCACATCCGAGTCTCTCTTTTGTTAATAGCAAGGATAGGGAGACACCGATTAAGAGTCAGACGATGCCATCATGTATCCCAAAGTTTGAATGTCTTCTTGGCCAGCAGATTGTTAAAATTGAGCTTCCGGTTGCTACCTCTTTTTACGGCACTGGGGAAGTTAGTGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAACACGGATGCGTGGGGCTATGGTTCTGGAACTACGTCATTGTACCAGTCGCATCCTTGGGTGCTGGCTGTTCTTCCTTCTGGGGAGGCTTTAGGAGTTCTTGCTGATACAACAAAGCGCTGTGAGATTGACTTAAGAAAAGAATCAGTGATACAGTTCATTGCTCCATCCTCTTATCCTATCATAACCTTTGGTCCATTTGCTTCACCCAATGCTGTTTTGATAGCTTTATCACATGCAATCGGTACTATATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAGTGTCGCTGGAGCTATGACACTGATAAGAGAGTTCAAGAGGTTGCAAGAACTTTTCGGGGGAAAAAGATTCCTTGTGATGTCATATGGATGGATATTGATTATATGGACGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAACGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAACAGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTTTGGATTTCGAAAGCAGACGGAACACCTTTTGTTGGGGAGGTCTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAAGCAAAAGCTCGATCATGGTGGTCCAACTTAGTCAAAGATTTCATTTCTAATGGTGTTGACGGTATATGGAATGACATGAATGAACCAGCTGTTTTTAAGGTTGTCACAAAAACAATGCCTGACAGCAATATCCATAAGGGGGATGAGGAACTGGGTGGTTGCCAGAATCACTCACACTACCACAATGTCTATGGATTGCTGATGGCAAGATCGACTTATGAAGGGATGAAGTTGGGTAATGAAAAGAAACGTCCTTTTGTCCTCACGAGGGCTGGATTTATTGGTAGCCAGCGTTATGCTGCAACATGGACCGGAGATAATCTTTCAACTTGGGAACACCTGCATATGAGTATTTCTATGGTACTTCAACTGCCGCTTTCAGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTCTTTGGAAGATGGATGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAACAGGACACCAGTGACCATGAGCCGTGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACTGCCTTATACCTCATTTATATACTCTCTTCTATATGGCTCATACGACCGGTACTCTAGTGGCAAGTCCTACATTTTTTGCTGATCCCAAAGACACCAGCTTAAGGAAAATGGAGACTTCTTTTTTGTTGGGTCCGGTTCTAGTGTACGCAAGCACTGCGGTTGGTCAGGGGATGGATACTTTGCGGTGCACATTGCCAAAAGGAATTTGGATGCGTTTTGATTTCGATGATGATTTACCAGCACTGTATTTGCAAGGCGGAGCAATTATTCCCGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTACTATACTAGATGATGCGACACTACTTGTGGCTTTGAATGAACATGGGAAAGCTGAAGGTGTATTATATGAAGATGATGGTGATGGATATGAGTTCCTGGAAGGAGGATTCTTGTTTACACATTATGTTGCTGAACTTCAATCATCAATGGTTATTGTCAAAGTTTCAAAAACTGTAGGATCTTGGAAGAGGCCAAACCGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCAATGGTTGATATATGGGGTAAGGATGGAGAGGTTCTTCAACTTTCAATGCCATCAGAACAGGAGGTCTCTCAGCTGGTTTCTACCAGTGAGAAACACTACCAAACTTGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGTGAGTTCTGCGCACAAAGGAATAGAGCTTTCAGGGACGCCTATTGAACTGAAAGGTGATGATTGGGCTATTAAAGTAGTGCCCTGGATTGGTGGGCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACTGAGTACCGCTCTGCTGGATGTTCCGAGGAATACAACGTTATTGAACGAAATCTTGAGCATGCTGGGGAGCAGGAATCTCTCATGTTAGAAGGTGATATAGGGGGTGGATTGGTTCTCCAACGACAGATATGTATTCCAAAGAATGATTCCAAGGTTTTCCGGATTGACTCTAGCATCATAGCACGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGACTGGTCTGCTTGAGAGTACACCCAATGTTCACCCTGTTGCACCCCTCAGAATCTTATGTTTCATTTATGGCTGTTGACGGATCAAAGCATGAAATTGGGCCTGAATCTAACGAGAAGTTCTATGAAGGGAATTTACTGCCTAATGGTGAATGGATGCTCGTTGATAAATGCCTTGGCTTGGGGTTACTGAATCGGTTTGATGTTAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATCTGGAGCTGTGGTCTGAGGACAGGCCAGTTTCAAACCAATCACCTCTTAGAATATCCCACGAGTATGAAGTGATAAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1064

Amino Acids

118.43

Weight (kDa)

5.84

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 159 - 233 1.6e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 274 - 603 1.9e-125 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 706 - 773 1.6e-09 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1551
Acc16I TGCGCA 1 cut(s) 2463
Acc36I ACCTGC 1 cut(s) 1551
AccBSI CCGCTC 2 cut(s) 541, 2646
AccI GTMKAC 1 cut(s) 209
AccIII TCCGGA 1 cut(s) 2796
AciI CCGC 8 cut(s) 362, 541, 1577, 1744, 1925, 1951, 2025, 2644
AclI AACGTT 1 cut(s) 2673
AclWI GGATC 5 cut(s) 39, 998, 1844, 2260, 2944
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 3 cut(s) 214, 1971, 2986
AcuI CTGAAG 3 cut(s) 270, 2148, 2559
AcyI GRCGYC 2 cut(s) 263, 2489
AfeI AGCGCT 1 cut(s) 686
AfiI CCNNNNNNNGG 9 cut(s) 834, 1157, 1342, 1471, 1583, 1595, 1869, 2540, 3034
AflII CTTAAG 2 cut(s) 700, 1867
AgeI ACCGGT 1 cut(s) 1814
AjnI CCWGG 4 cut(s) 1066, 1171, 2169, 2534
AjuI GAANNNNNNNTTGG 4 cut(s) 444, 476, 2767, 2799
Alw21I GWGCWC 2 cut(s) 1629, 1958
Alw26I GTCTC 4 cut(s) 380, 398, 1874, 2380
Alw44I GTGCAC 1 cut(s) 1954
AlwI GGATC 5 cut(s) 39, 998, 1844, 2260, 2944
AlwNI CAGNNNCTG 1 cut(s) 1589
Aor13HI TCCGGA 1 cut(s) 2796
Aor51HI AGCGCT 1 cut(s) 686
AoxI GGCC 7 cut(s) 463, 1169, 1175, 1586, 2264, 2954, 3131
ApaLI GTGCAC 1 cut(s) 1954
ApeKI GCWGC 1 cut(s) 1502
ApoI RAATTY 3 cut(s) 214, 1971, 2986
ArsI GACNNNNNNTTYG 2 cut(s) 1873, 1905
AseI ATTAAT 1 cut(s) 2610
AsiGI ACCGGT 1 cut(s) 1814
Asp700I GAANNNNTTC 2 cut(s) 456, 2554
AspLEI GCGC 3 cut(s) 687, 1764, 2464
AsuII TTCGAA 1 cut(s) 1133
AvaII GGWCC 7 cut(s) 761, 837, 991, 1224, 1512, 1898, 2045
BaeGI GKGCMC 4 cut(s) 124, 1680, 1958, 2535
BaeI ACNNNNGTAYC 2 cut(s) 1809, 1842
BalI TGGCCA 1 cut(s) 465
BanII GRGCYC 1 cut(s) 1629
BarI GAAGNNNNNNTAC 2 cut(s) 2973, 3005
BauI CACGAG 2 cut(s) 1468, 3167
BbsI GAAGAC 3 cut(s) 68, 173, 449
Bbv12I GWGCWC 2 cut(s) 1629, 1958
BbvI GCAGC 1 cut(s) 1489
BceAI ACGGC 3 cut(s) 526, 1656, 1693
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 4 cut(s) 1068, 1173, 2171, 2536
BciVI GTATCC 2 cut(s) 449, 1936
BcoDI GTCTC 4 cut(s) 380, 398, 1874, 2380
BfaI CTAG 6 cut(s) 225, 1823, 1907, 2081, 2808, 3190
BfmI CTRYAG 1 cut(s) 2247
BfoI RGCGCY 2 cut(s) 688, 1765
BfrI CTTAAG 2 cut(s) 700, 1867
BfuAI ACCTGC 1 cut(s) 1551
BfuI GTATCC 2 cut(s) 449, 1936
BisI GCNGC 4 cut(s) 363, 1503, 1577, 1744
BlsI GCNGC 4 cut(s) 364, 1504, 1578, 1745
Bme1390I CCNGG 4 cut(s) 1068, 1173, 2171, 2536
Bme18I GGWCC 7 cut(s) 761, 837, 991, 1224, 1512, 1898, 2045
BmiI GGNNCC 2 cut(s) 1899, 2046
BmrFI CCNGG 4 cut(s) 1068, 1173, 2171, 2536
BmrI ACTGGG 4 cut(s) 111, 525, 1364, 3097
BmsI GCATC 8 cut(s) 417, 565, 628, 1047, 1968, 2077, 2820, 3000
BmuI ACTGGG 4 cut(s) 111, 525, 1364, 3097
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 3 cut(s) 68, 173, 449
BplI GAGNNNNNCTC 2 cut(s) 2630, 2662
BpmI CTGGAG 3 cut(s) 34, 883, 3131
Bpu10I CCTNAGC 2 cut(s) 149, 1623
Bpu14I TTCGAA 1 cut(s) 1133
BpuEI CTTGAG 3 cut(s) 557, 2711, 2887
BsaHI GRCGYC 2 cut(s) 263, 2489
BsaI GGTCTC 1 cut(s) 2380
BsaWI WCCGGW 5 cut(s) 490, 1514, 1814, 1900, 2796
BsaXI ACNNNNNCTCC 6 cut(s) 97, 127, 160, 190, 3104, 3134
Bsc4I CCNNNNNNNGG 9 cut(s) 834, 1157, 1342, 1471, 1583, 1595, 1869, 2540, 3034
Bse118I RCCGGY 1 cut(s) 1814
Bse3DI GCAATG 3 cut(s) 726, 1958, 2316
BseAI TCCGGA 1 cut(s) 2796
BseBI CCWGG 4 cut(s) 1068, 1173, 2171, 2536
BseLI CCNNNNNNNGG 9 cut(s) 834, 1157, 1342, 1471, 1583, 1595, 1869, 2540, 3034
BseMI GCAATG 3 cut(s) 726, 1958, 2316
BseMII CTCAG 6 cut(s) 163, 1614, 2393, 2628, 2916, 3114
BseRI GAGGAG 2 cut(s) 1771, 2040
BseSI GKGCMC 4 cut(s) 124, 1680, 1958, 2535
BseXI GCAGC 1 cut(s) 1489
BseYI CCCAGC 1 cut(s) 2699
BsgI GTGCAG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 1815
BshFI GGCC 7 cut(s) 465, 1171, 1177, 1588, 2266, 2956, 3133
BshTI ACCGGT 1 cut(s) 1814
BsiEI CGRYCG 1 cut(s) 1815
BsiHKAI GWGCWC 2 cut(s) 1629, 1958
BsiSI CCGG 5 cut(s) 491, 1515, 1815, 1901, 2797
BslFI GGGAC 3 cut(s) 274, 2500, 2593
BslI CCNNNNNNNGG 9 cut(s) 834, 1157, 1342, 1471, 1583, 1595, 1869, 2540, 3034
BsmAI GTCTC 4 cut(s) 380, 398, 1874, 2380
BsmFI GGGAC 3 cut(s) 274, 2500, 2593
BsnI GGCC 7 cut(s) 465, 1171, 1177, 1588, 2266, 2956, 3133
Bso31I GGTCTC 1 cut(s) 2380
Bsp119I TTCGAA 1 cut(s) 1133
Bsp1286I GDGCHC 5 cut(s) 124, 1629, 1680, 1958, 2535
Bsp13I TCCGGA 1 cut(s) 2796
Bsp143I GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
BspACI CCGC 8 cut(s) 362, 541, 1577, 1744, 1925, 1951, 2025, 2644
BspANI GGCC 7 cut(s) 465, 1171, 1177, 1588, 2266, 2956, 3133
BspCNI CTCAG 6 cut(s) 162, 1615, 2392, 2629, 2915, 3115
BspEI TCCGGA 1 cut(s) 2796
BspLI GGNNCC 2 cut(s) 1899, 2046
BspMI ACCTGC 1 cut(s) 1551
BspPI GGATC 5 cut(s) 39, 998, 1844, 2260, 2944
BspT104I TTCGAA 1 cut(s) 1133
BspTI CTTAAG 2 cut(s) 700, 1867
BspTNI GGTCTC 1 cut(s) 2380
BsrBI CCGCTC 2 cut(s) 541, 2646
BsrDI GCAATG 3 cut(s) 726, 1958, 2316
BsrFI RCCGGY 1 cut(s) 1814
BssAI RCCGGY 1 cut(s) 1814
BssMI GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
BssNI GRCGYC 2 cut(s) 263, 2489
BssSI CACGAG 2 cut(s) 1468, 3167
BssT1I CCWWGG 5 cut(s) 623, 840, 1014, 2787, 3028
Bst2BI CACGAG 2 cut(s) 1468, 3167
Bst2UI CCWGG 4 cut(s) 1068, 1173, 2171, 2536
Bst6I CTCTTC 3 cut(s) 1719, 1799, 2255
BstACI GRCGYC 2 cut(s) 263, 2489
BstAFI CTTAAG 2 cut(s) 700, 1867
BstAPI GCANNNNNTGC 2 cut(s) 1922, 2293
BstBI TTCGAA 1 cut(s) 1133
BstC8I GCNNGC 7 cut(s) 467, 633, 1493, 1918, 2279, 2697, 2821
BstDEI CTNAG 8 cut(s) 149, 1231, 1623, 2379, 2637, 2902, 3123, 3155
BstDSI CCRYGG 3 cut(s) 1672, 1709, 2040
BstH2I RGCGCY 2 cut(s) 688, 1765
BstHHI GCGC 3 cut(s) 687, 1764, 2464
BstKTI GATC 9 cut(s) 47, 239, 1006, 1027, 1217, 1415, 1852, 2255, 2939
BstMAI GTCTC 4 cut(s) 380, 398, 1874, 2380
BstMBI GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
BstMCI CGRYCG 1 cut(s) 1815
BstMWI GCNNNNNNNGC 6 cut(s) 128, 538, 1585, 1613, 1922, 2293
BstNI CCWGG 4 cut(s) 1068, 1173, 2171, 2536
BstNSI RCATGY 4 cut(s) 806, 2063, 2285, 2699
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 1066, 1171, 2169, 2534
BstSFI CTRYAG 1 cut(s) 2247
BstSLI GKGCMC 4 cut(s) 124, 1680, 1958, 2535
BstV1I GCAGC 1 cut(s) 1489
BstV2I GAAGAC 3 cut(s) 68, 173, 449
BstX2I RGATCY 2 cut(s) 1003, 2252
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 2 cut(s) 1003, 2252
BsuI GTATCC 2 cut(s) 449, 1936
BsuRI GGCC 7 cut(s) 465, 1171, 1177, 1588, 2266, 2956, 3133
BtgI CCRYGG 3 cut(s) 1672, 1709, 2040
BtgZI GCGATG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 1920
BveI ACCTGC 1 cut(s) 1551
Cac8I GCNNGC 7 cut(s) 467, 633, 1493, 1918, 2279, 2697, 2821
CaiI CAGNNNCTG 1 cut(s) 1589
CfoI GCGC 3 cut(s) 687, 1764, 2464
Cfr10I RCCGGY 1 cut(s) 1814
CseI GACGC 2 cut(s) 271, 2497
CspAI ACCGGT 1 cut(s) 1814
DdeI CTNAG 8 cut(s) 149, 1231, 1623, 2379, 2637, 2902, 3123, 3155
DpnI GATC 9 cut(s) 46, 238, 1005, 1026, 1216, 1414, 1851, 2254, 2938
DpnII GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 3 cut(s) 1719, 1799, 2255
EarI CTCTTC 3 cut(s) 1719, 1799, 2255
EciI GGCGGA 1 cut(s) 2040
Ecl136II GAGCTC 1 cut(s) 1627
Eco130I CCWWGG 5 cut(s) 623, 840, 1014, 2787, 3028
Eco24I GRGCYC 1 cut(s) 1629
Eco31I GGTCTC 1 cut(s) 2380
Eco47I GGWCC 7 cut(s) 761, 837, 991, 1224, 1512, 1898, 2045
Eco47III AGCGCT 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 1627
Eco57I CTGAAG 3 cut(s) 270, 2148, 2559
EcoICRI GAGCTC 1 cut(s) 1627
EcoO109I RGGNCCY 2 cut(s) 1586, 2045
EcoRII CCWGG 4 cut(s) 1066, 1171, 2169, 2534
EcoT14I CCWWGG 5 cut(s) 623, 840, 1014, 2787, 3028
EcoT38I GRGCYC 1 cut(s) 1629
ErhI CCWWGG 5 cut(s) 623, 840, 1014, 2787, 3028
FalI AAGNNNNNCTT 4 cut(s) 824, 856, 1402, 1434
FaqI GGGAC 3 cut(s) 274, 2500, 2593
FauNDI CATATG 2 cut(s) 938, 1548
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 4 cut(s) 363, 1503, 1577, 1744
FriOI GRGCYC 1 cut(s) 1629
Fsp4HI GCNGC 4 cut(s) 363, 1503, 1577, 1744
FspBI CTAG 6 cut(s) 225, 1823, 1907, 2081, 2808, 3190
FspI TGCGCA 1 cut(s) 2463
GlaI GCGC 3 cut(s) 686, 1763, 2463
GluI GCNGC 4 cut(s) 363, 1503, 1577, 1744
GsaI CCCAGC 1 cut(s) 2703
GsuI CTGGAG 3 cut(s) 34, 883, 3131
HaeII RGCGCY 2 cut(s) 688, 1765
HaeIII GGCC 7 cut(s) 465, 1171, 1177, 1588, 2266, 2956, 3133
HapII CCGG 5 cut(s) 491, 1515, 1815, 1901, 2797
HgaI GACGC 2 cut(s) 271, 2497
HhaI GCGC 3 cut(s) 687, 1764, 2464
Hin1I GRCGYC 2 cut(s) 263, 2489
Hin6I GCGC 3 cut(s) 685, 1762, 2462
HinP1I GCGC 3 cut(s) 685, 1762, 2462
HincII GTYRAC 2 cut(s) 1261, 2932
HindII GTYRAC 2 cut(s) 1261, 2932
HpaII CCGG 5 cut(s) 491, 1515, 1815, 1901, 2797
Hpy166II GTNNAC 9 cut(s) 210, 1021, 1261, 1912, 1956, 2190, 2875, 2886, 2932
Hpy8I GTNNAC 9 cut(s) 210, 1021, 1261, 1912, 1956, 2190, 2875, 2886, 2932
Hpy99I CGWCG 1 cut(s) 2277
HpyCH4IV ACGT 3 cut(s) 602, 1453, 2673
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 538, 1585, 1613, 1922, 2293
HpyF3I CTNAG 8 cut(s) 149, 1231, 1623, 2379, 2637, 2902, 3123, 3155
HpySE526I ACGT 3 cut(s) 602, 1453, 2673
Hsp92I GRCGYC 2 cut(s) 263, 2489
HspAI GCGC 3 cut(s) 685, 1762, 2462
Kpn2I TCCGGA 1 cut(s) 2796
Kzo9I GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
LmnI GCTCC 5 cut(s) 736, 864, 2027, 2704, 3112
Lsp1109I GCAGC 1 cut(s) 1489
LweI GCATC 8 cut(s) 417, 565, 628, 1047, 1968, 2077, 2820, 3000
MaeI CTAG 6 cut(s) 225, 1823, 1907, 2081, 2808, 3190
MaeII ACGT 3 cut(s) 602, 1453, 2673
MaeIII GTNAC 5 cut(s) 220, 280, 1306, 1697, 3039
MalI GATC 9 cut(s) 46, 238, 1005, 1026, 1216, 1414, 1851, 2254, 2938
MbiI CCGCTC 2 cut(s) 541, 2646
MboI GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
MflI RGATCY 2 cut(s) 1003, 2252
MhlI GDGCHC 5 cut(s) 124, 1629, 1680, 1958, 2535
MlsI TGGCCA 1 cut(s) 465
MluCI AATT 7 cut(s) 214, 480, 1971, 2031, 2288, 2949, 2986
MluNI TGGCCA 1 cut(s) 465
MlyI GAGTC 4 cut(s) 382, 427, 2557, 2798
MmeI TCCRAC 2 cut(s) 1251, 2779
Mox20I TGGCCA 1 cut(s) 465
MroI TCCGGA 1 cut(s) 2796
MroXI GAANNNNTTC 2 cut(s) 456, 2554
MscI TGGCCA 1 cut(s) 465
MseI TTAA 9 cut(s) 387, 414, 477, 701, 1299, 1868, 2523, 2610, 3105
MslI CAYNNNNRTG 6 cut(s) 941, 1035, 2058, 2064, 2558, 3171
Msp20I TGGCCA 1 cut(s) 465
MspA1I CMGCKG 2 cut(s) 1292, 2383
MspCI CTTAAG 2 cut(s) 700, 1867
MspI CCGG 5 cut(s) 491, 1515, 1815, 1901, 2797
MspR9I CCNGG 4 cut(s) 1068, 1173, 2171, 2536
MvaI CCWGG 4 cut(s) 1068, 1173, 2171, 2536
MwoI GCNNNNNNNGC 6 cut(s) 128, 538, 1585, 1613, 1922, 2293
NdeI CATATG 2 cut(s) 938, 1548
NdeII GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
NlaIV GGNNCC 2 cut(s) 1899, 2046
NmuCI GTSAC 3 cut(s) 280, 1306, 1697
NsbI TGCGCA 1 cut(s) 2463
NspI RCATGY 4 cut(s) 806, 2063, 2285, 2699
NspV TTCGAA 1 cut(s) 1133
PaeI GCATGC 1 cut(s) 2699
PaqCI CACCTGC 1 cut(s) 1551
PasI CCCWGGG 1 cut(s) 1067
PdmI GAANNNNTTC 2 cut(s) 456, 2554
PfoI TCCNGGA 1 cut(s) 2169
PinAI ACCGGT 1 cut(s) 1814
PkrI GCNGC 4 cut(s) 364, 1504, 1578, 1745
PleI GAGTC 4 cut(s) 381, 426, 2557, 2798
PpsI GAGTC 4 cut(s) 381, 426, 2557, 2798
PpuMI RGGWCCY 1 cut(s) 2045
PshAI GACNNNNGTC 1 cut(s) 266
PshBI ATTAAT 1 cut(s) 2610
Psp124BI GAGCTC 1 cut(s) 1629
Psp1406I AACGTT 1 cut(s) 2673
Psp5II RGGWCCY 1 cut(s) 2045
Psp6I CCWGG 4 cut(s) 1066, 1171, 2169, 2534
PspFI CCCAGC 1 cut(s) 2699
PspGI CCWGG 4 cut(s) 1066, 1171, 2169, 2534
PspN4I GGNNCC 2 cut(s) 1899, 2046
PspPPI RGGWCCY 1 cut(s) 2045
PstNI CAGNNNCTG 1 cut(s) 1589
PsuI RGATCY 2 cut(s) 1003, 2252
PvuII CAGCTG 2 cut(s) 1292, 2383
RseI CAYNNNNRTG 6 cut(s) 941, 1035, 2058, 2064, 2558, 3171
SacI GAGCTC 1 cut(s) 1629
SaqAI TTAA 9 cut(s) 387, 414, 477, 701, 1299, 1868, 2523, 2610, 3105
SatI GCNGC 4 cut(s) 363, 1503, 1577, 1744
Sau3AI GATC 9 cut(s) 44, 236, 1003, 1024, 1214, 1412, 1849, 2252, 2936
SchI GAGTC 4 cut(s) 382, 427, 2557, 2798
ScrFI CCNGG 4 cut(s) 1068, 1173, 2171, 2536
SduI GDGCHC 5 cut(s) 124, 1629, 1680, 1958, 2535
SfaNI GCATC 8 cut(s) 417, 565, 628, 1047, 1968, 2077, 2820, 3000
SfcI CTRYAG 1 cut(s) 2247
SfuI TTCGAA 1 cut(s) 1133
SinI GGWCC 7 cut(s) 761, 837, 991, 1224, 1512, 1898, 2045
SmiMI CAYNNNNRTG 6 cut(s) 941, 1035, 2058, 2064, 2558, 3171
SmlI CTYRAG 5 cut(s) 536, 700, 1867, 2690, 2866
SmoI CTYRAG 5 cut(s) 536, 700, 1867, 2690, 2866
SphI GCATGC 1 cut(s) 2699
Sse9I AATT 7 cut(s) 214, 480, 1971, 2031, 2288, 2949, 2986
SsiI CCGC 8 cut(s) 362, 541, 1577, 1744, 1925, 1951, 2025, 2644
SspMI CTAG 6 cut(s) 225, 1823, 1907, 2081, 2808, 3190
SstI GAGCTC 1 cut(s) 1629
StyD4I CCNGG 4 cut(s) 1066, 1171, 2169, 2534
StyI CCWWGG 5 cut(s) 623, 840, 1014, 2787, 3028
TaiI ACGT 3 cut(s) 605, 1456, 2676
TaqI TCGA 8 cut(s) 239, 303, 315, 331, 1133, 1213, 1415, 1992
TasI AATT 7 cut(s) 214, 480, 1971, 2031, 2288, 2949, 2986
TatI WGTACW 1 cut(s) 2872
TauI GCSGC 3 cut(s) 365, 1579, 1746
Tru1I TTAA 9 cut(s) 387, 414, 477, 701, 1299, 1868, 2523, 2610, 3105
Tru9I TTAA 9 cut(s) 387, 414, 477, 701, 1299, 1868, 2523, 2610, 3105
TseFI GTSAC 3 cut(s) 280, 1306, 1697
TseI GCWGC 1 cut(s) 1502
Tsp45I GTSAC 3 cut(s) 280, 1306, 1697
TspGWI ACGGA 4 cut(s) 174, 587, 1158, 2949
Vha464I CTTAAG 2 cut(s) 700, 1867
VneI GTGCAC 1 cut(s) 1954
VpaK11BI GGWCC 7 cut(s) 761, 837, 991, 1224, 1512, 1898, 2045
VspI ATTAAT 1 cut(s) 2610
XapI RAATTY 3 cut(s) 214, 1971, 2986
XceI RCATGY 4 cut(s) 806, 2063, 2285, 2699
XcmI CCANNNNNNNNNTGG 3 cut(s) 1391, 1971, 3092
XmiI GTMKAC 1 cut(s) 209
XmnI GAANNNNTTC 2 cut(s) 456, 2554
XspI CTAG 6 cut(s) 225, 1823, 1907, 2081, 2808, 3190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.