MD00G1148100.v1.1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005660
Physical Location & Seq
Forward (+)
32473530 .. 32488848
15319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1148100.v1.1.491

Sequence Viewer

Length: 3174 bp
ATGGGAGAGGTAGGAGTAGCAGTGAGTGGCAGTGTAGTTGGTGGCGGAGGAGGATCATCGAAATTCGCTCTGAGGCATCCTCTTTCTCCGTTTGCGGTGGTCGGATTGAGGAACCTCCGTGCTCTTGAATTTGGGATCATCACCATCAGAAAGAAGAGGTCTGCTGAAAGGTTGGTTACTGAATGTTTGGTATCTAAAATGGCTGATTACAAAGGGAAGGCAGTCGCTGCAGATGCCACGTCAGGAGCTATGATTTTCGAGCCTATTGTTGAGGATGGAATATTCCGATTTGATTGTTCTGCAAATGATCGAAATGCAGCACATCCTAGTGTCTCTTTTACTAATAGCAAGGATAGAGAAACACCAATAATGAATCACAACACACCTTCATATGTCCCAAGTTTCGAATGCCTTTTGGGGCAGCAGATTGTCAAACTTGAGCTTCCTTCCGGGACCTCTTTCTATGGAACTGGGGAAGTTAGTGGGCAACTTGAGCGAACAGGGAAAAGAGTTTTTACTTGGAACACGGATGCATGGGGCTATGGTCCTGGAACTACATCATTGTATCAGTCACATCCTTGGGTGCTGGCTGTTCTTCCAACTGGAGAGGCATTAGGAATTCTTGCTGATACAACACGGCGCTGTGAGATTGATCTGAGGAAAGAATCGATGATACAGTTCATTGCTCCATCCTCTTATCCGGTCGTTACATTCGGTCCATTTGCTTCACCTGATGGTGTTTTGATATCTTTATCACATGTAATTGGGACTGTATTTATGCCCCCAAAGTGGTCCTTAGGCTATCATCAATGTCGTTGGAGCTATGACTCTGATAAGAAAGTTCAAGAGATTACAAGAACATTTCGAGCGAAGGGTATTCCTTGTGACGTCATATGGATGGATATTGATTACATGGATGGCTTTCGTTGTTTCACTTTTGACAAGGAGCGCTTTCCAGATCCGAAATCATTGGTAAAGGGTCTAAATCAAAATGGTATCAAGGCCATCTGGATGCTTGACCCAGGGATAAAACAGGAAGATGGTTATTTTGTCTATGATAGTGGTTGTAAAAGTGATGTCTGGATTTCAAGGGCAGATGGAAGACCATTTGTTGGGGACGTGTGGCCTGGGCCTTGTGTTTTTCCAGACTACACACAGGCAAAAGTTCGATCTTGGTGGTCCAATTTAGTCAAAGATTTCACTTTAAATGGTGTTGACGGAATATGGAATGATATGAATGAACCAGCTGTTTTTAAGACTGTCACAAAAACGATGCCCGAGAGCAATATTCATAAGGGGGATGATGAACTTGGAGGGTGTCAGAATCATTCACACTATCACAATGTCTATGGCATGCTAATGGCAAGGTCTACTTATGAAGGAATGAAGCTGGGTAATGAAAAAAAACGCCCTTTTGTTCTCACCAGAGCTGGATTTAGTGGTAGCCAACGGTATGCTGCAACGTGGACAGGAGATAATCTTTCAACTTGGGAGCACCTCCATATGAGCATTTCCATGGTTCTTCAATTGGGACTTAGTGGTCAGCCACTATCAGGGCCTGATATTGGTGGTTTTGGTGGAAATGCAACACCCAAGCTCTTTGGACGGTGGATGGGTATAGGTTCTATGTTTCCCTTTTGCCGTGGGCACTCTGAAATGGACACGATTGACCATGAGCCCTGGTCGTTTGGGGAAGAGTGTGAAGAAGTATGCCGTTTGGCATTAATTAGGCGCTACCGTCTTCTACCACATATTTACACTCTCTTCTATATGGCTCATACAACAGGCACTCCAGTGGCAAGTCCTACATTTTTTGCTGATCTCAAGGACTCCAGCTTAAGGAAATTGGAGAATTCTTTTTTGTTGGGTCCACTTCTAGTCATTGCCAGCACTGAGCCTGGTCAGGGGATGGATAGTTTGCAGTTCACATTGCCCAAAGGAATTTGGTTGAGTTTTGATTTTGGTGATTCACATCCAGATTTACCAGCTTTATATTTGCAAGGTGGAGCAATTATTCCCGTGGGTCCTCCTCATCAGCATCTTGGCGAATCTGATCAATTAGAAGACTTGACACTCCTTGTGGCTTTAGATGAAGATGGGAAAGCTAAAGGTGTTCTCTATGAAGATGATGGTGATGGATACGAGTTCATGAATGGTGGATTCTTGTTGACACACTATATTGCTGAACTTCAATCTTCTACTGTTACCGTTAAAGTTTTAAAAACTGAAGGATCTTGGAAGAGGCCAAAACGTCGGCTACATGTGCAATTATTGCTTGGTGGAGGGGCAATGGTTGATACATGGGGCAATGATGGAGAGGTTCTTCAAATTGTAATGCCTTCAGAACAGGAAGTTGGGAAGCTGGTTTCCACTAGTGAGAAGCAATACCGAACTCGTTTGGAAACTGCCAAGCCTATTCCAGATGCGGAAGTGACTTCTGCGCACAAAGGAATAGAACTTTCGAGGATTCCTATTGAACTGAAAGGTGGTGATTGGGATATTAAAGTCGTTCCCTGGATTGGTGGTAGAATTATTTCCATGATGCACCGCCCTTCAGGGACACAATGGCTTCACAGTAGAGTTGACATTAATGGGTATGAAGAGTATAGTGGTACTGAGTACCGCTCTGCTGGATGTACTGAGGAATACAATGTTACAGAACGGAATCTTGAGCATGCTGGAGAGGAGGAATCTATTCTGTTAGAAGGTGATATTGGGGGTGGTTTGGTTCTCCAACGACAGTTACATATTCCAAAGAATGATCCCAAGGTTTTCCGAATTGACTCTAGCATTATAGCATGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGGCTGGTCTGCCTGAGAGTACACCCAATGTTCACCCTCTTGCACCCTACAGAATCTTATGTCTCATTTACAGCCATCGATGGGTCAAAGCATGAAATTTGGCCTGAATCCGATGAGCAGTTGTTTCAAGGGGATTTACTGCCTAATGGTGAATGGATGCTCATCGATAAATGCGTTGGGTTGGGGCTTGTGAATCGCTTTGATGCTAGCCAAGTTTACAAGTGCCTAATCCATTGGGGAACTGGAACTGTTAATTTGGAGCTGTGGTCCGAGGACAGGCCGGTTTCTAAGCAATCACCTCTTAGAATTGCCCACGAGTATGAGGTTGTGACACTCCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1058

Amino Acids

117.25

Weight (kDa)

5.67

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 144 - 218 2.3e-17 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 259 - 588 5.5e-123 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 690 - 758 2.4e-12 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1539
AatII GACGTC 1 cut(s) 891
Acc16I TGCGCA 1 cut(s) 2442
AccB7I CCANNNNNTGG 1 cut(s) 1112
AccBSI CCGCTC 1 cut(s) 2625
AccI GTMKAC 1 cut(s) 1370
AciI CCGC 5 cut(s) 45, 95, 2426, 2548, 2623
AclWI GGATC 5 cut(s) 61, 143, 953, 2239, 2756
AcsI RAATTY 6 cut(s) 62, 128, 618, 1852, 1941, 2928
AcuI CTGAAG 3 cut(s) 2247, 2325, 2538
AcyI GRCGYC 1 cut(s) 888
AfaI GTAC 4 cut(s) 2614, 2621, 2638, 2853
AfeI AGCGCT 1 cut(s) 950
AfiI CCNNNNNNNGG 9 cut(s) 789, 1112, 1553, 1565, 1839, 1904, 2519, 2913, 3108
AflII CTTAAG 1 cut(s) 1837
AflIII ACRYGT 3 cut(s) 757, 1119, 2260
AhlI ACTAGT 1 cut(s) 2372
AjiI CACGTC 2 cut(s) 240, 1120
AjnI CCWGG 6 cut(s) 547, 1021, 1126, 1679, 1897, 2513
AjuI GAANNNNNNNTTGG 4 cut(s) 2337, 2369, 2697, 2729
AleI CACNNNNGTG 1 cut(s) 1793
Alw21I GWGCWC 2 cut(s) 124, 1497
Alw26I GTCTC 2 cut(s) 337, 2899
AlwI GGATC 5 cut(s) 61, 143, 953, 2239, 2756
AlwNI CAGNNNCTG 2 cut(s) 227, 1559
Ama87I CYCGRG 1 cut(s) 1277
Aor51HI AGCGCT 1 cut(s) 950
AoxI GGCC 7 cut(s) 1002, 1124, 1130, 1556, 2243, 2933, 3110
ApeKI GCWGC 4 cut(s) 227, 317, 421, 1457
ApoI RAATTY 6 cut(s) 62, 128, 618, 1852, 1941, 2928
AseI ATTAAT 2 cut(s) 1724, 2589
Asp700I GAANNNNTTC 2 cut(s) 2533, 2694
AspLEI GCGC 4 cut(s) 642, 951, 1734, 2443
AsuC2I CCSGG 1 cut(s) 451
AsuII TTCGAA 1 cut(s) 405
AsuNHI GCTAGC 1 cut(s) 3038
AvaI CYCGRG 1 cut(s) 1277
AvaII GGWCC 8 cut(s) 453, 545, 716, 792, 1179, 1868, 2024, 3099
AxyI CCTNAGG 1 cut(s) 796
BaeGI GKGCMC 1 cut(s) 1650
BanII GRGCYC 1 cut(s) 1680
BauI CACGAG 1 cut(s) 3146
BbsI GAAGAC 3 cut(s) 1108, 1733, 2070
Bbv12I GWGCWC 2 cut(s) 124, 1497
BbvI GCAGC 4 cut(s) 214, 329, 433, 1444
BceAI ACGGC 3 cut(s) 653, 1626, 1698
BciT130I CCWGG 6 cut(s) 549, 1023, 1128, 1681, 1899, 2515
BciVI GTATCC 1 cut(s) 2132
BclI TGATCA 1 cut(s) 2053
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 337, 2899
BcuI ACTAGT 1 cut(s) 2372
BfaI CTAG 5 cut(s) 327, 1877, 2373, 2787, 3039
BfmI CTRYAG 2 cut(s) 228, 2880
BfoI RGCGCY 3 cut(s) 643, 952, 1735
BfrI CTTAAG 1 cut(s) 1837
BfuI GTATCC 1 cut(s) 2132
BisI GCNGC 4 cut(s) 228, 318, 422, 1458
BlsI GCNGC 4 cut(s) 229, 319, 423, 1459
Bme1390I CCNGG 7 cut(s) 451, 549, 1023, 1128, 1681, 1899, 2515
Bme18I GGWCC 8 cut(s) 453, 545, 716, 792, 1179, 1868, 2024, 3099
BmeT110I CYCGRG 1 cut(s) 1277
BmgBI CACGTC 2 cut(s) 240, 1120
BmiI GGNNCC 4 cut(s) 113, 454, 1869, 2025
BmrFI CCNGG 7 cut(s) 451, 549, 1023, 1128, 1681, 1899, 2515
BmrI ACTGGG 1 cut(s) 480
BmtI GCTAGC 1 cut(s) 3042
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 3 cut(s) 1108, 1733, 2070
BplI GAGNNNNNCTC 4 cut(s) 64, 96, 2609, 2641
BpmI CTGGAG 4 cut(s) 624, 1776, 1816, 2700
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 4 cut(s) 458, 512, 1808, 2690
BpuMI CCSGG 1 cut(s) 451
Bsa29I ATCGAT 3 cut(s) 668, 2910, 2997
BsaBI GATNNNNATC 2 cut(s) 1971, 2993
BsaHI GRCGYC 1 cut(s) 888
BsaWI WCCGGW 1 cut(s) 700
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 1774, 1804, 3083, 3113
Bsc4I CCNNNNNNNGG 9 cut(s) 789, 1112, 1553, 1565, 1839, 1904, 2519, 2913, 3108
Bse118I RCCGGY 1 cut(s) 3112
Bse1I ACTGG 4 cut(s) 475, 607, 1793, 3079
Bse21I CCTNAGG 1 cut(s) 796
Bse3DI GCAATG 5 cut(s) 681, 1881, 1928, 2295, 2314
Bse8I GATNNNNATC 2 cut(s) 1971, 2993
BseBI CCWGG 6 cut(s) 549, 1023, 1128, 1681, 1899, 2515
BseCI ATCGAT 3 cut(s) 668, 2910, 2997
BseJI GATNNNNATC 2 cut(s) 1971, 2993
BseLI CCNNNNNNNGG 9 cut(s) 789, 1112, 1553, 1565, 1839, 1904, 2519, 2913, 3108
BseMI GCAATG 5 cut(s) 681, 1881, 1928, 2295, 2314
BseMII CTCAG 6 cut(s) 62, 647, 1884, 2607, 2631, 2837
BseNI ACTGG 4 cut(s) 475, 607, 1793, 3079
BseRI GAGGAG 3 cut(s) 63, 2019, 2699
BseSI GKGCMC 1 cut(s) 1650
BseXI GCAGC 4 cut(s) 214, 329, 433, 1444
BseYI CCCAGC 1 cut(s) 1390
Bsh1285I CGRYCG 1 cut(s) 705
BshFI GGCC 7 cut(s) 1004, 1126, 1132, 1558, 2245, 2935, 3112
BshVI ATCGAT 3 cut(s) 668, 2910, 2997
BsiEI CGRYCG 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 124, 1497
BsiHKCI CYCGRG 1 cut(s) 1277
BsiSI CCGG 3 cut(s) 450, 701, 3113
BslFI GGGAC 6 cut(s) 380, 466, 781, 1130, 1545, 2572
BslI CCNNNNNNNGG 9 cut(s) 789, 1112, 1553, 1565, 1839, 1904, 2519, 2913, 3108
BsmAI GTCTC 2 cut(s) 337, 2899
BsmFI GGGAC 6 cut(s) 380, 466, 781, 1130, 1545, 2572
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 7 cut(s) 1004, 1126, 1132, 1558, 2245, 2935, 3112
BsoBI CYCGRG 1 cut(s) 1277
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 4 cut(s) 124, 1497, 1650, 1680
Bsp19I CCATGG 1 cut(s) 1515
BspACI CCGC 5 cut(s) 45, 95, 2426, 2548, 2623
BspANI GGCC 7 cut(s) 1004, 1126, 1132, 1558, 2245, 2935, 3112
BspCNI CTCAG 6 cut(s) 63, 648, 1885, 2608, 2632, 2838
BspDI ATCGAT 3 cut(s) 668, 2910, 2997
BspHI TCATGA 1 cut(s) 2148
BspLI GGNNCC 4 cut(s) 113, 454, 1869, 2025
BspMAI CTGCAG 1 cut(s) 232
BspOI GCTAGC 1 cut(s) 3042
BspPI GGATC 5 cut(s) 61, 143, 953, 2239, 2756
BspT104I TTCGAA 1 cut(s) 405
BspTI CTTAAG 1 cut(s) 1837
BsrBI CCGCTC 1 cut(s) 2625
BsrDI GCAATG 5 cut(s) 681, 1881, 1928, 2295, 2314
BsrFI RCCGGY 1 cut(s) 3112
BsrI ACTGG 4 cut(s) 475, 607, 1793, 3079
BssAI RCCGGY 1 cut(s) 3112
BssNI GRCGYC 1 cut(s) 888
BssSI CACGAG 1 cut(s) 3146
BssT1I CCWWGG 3 cut(s) 578, 1515, 2766
Bst2BI CACGAG 1 cut(s) 3146
Bst2UI CCWGG 6 cut(s) 549, 1023, 1128, 1681, 1899, 2515
Bst6I CTCTTC 5 cut(s) 149, 1689, 1769, 2234, 2595
BstACI GRCGYC 1 cut(s) 888
BstAFI CTTAAG 1 cut(s) 1837
BstAPI GCANNNNNTGC 2 cut(s) 227, 2272
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 6 cut(s) 588, 1355, 1888, 2676, 2800, 3040
BstDSI CCRYGG 3 cut(s) 1515, 1642, 2019
BstH2I RGCGCY 3 cut(s) 643, 952, 1735
BstHHI GCGC 4 cut(s) 642, 951, 1734, 2443
BstMAI GTCTC 2 cut(s) 337, 2899
BstMCI CGRYCG 1 cut(s) 705
BstMWI GCNNNNNNNGC 5 cut(s) 227, 233, 493, 2263, 2272
BstNI CCWGG 6 cut(s) 549, 1023, 1128, 1681, 1899, 2515
BstNSI RCATGY 5 cut(s) 761, 1357, 2264, 2678, 2802
BstSCI CCNGG 7 cut(s) 449, 547, 1021, 1126, 1679, 1897, 2513
BstSFI CTRYAG 2 cut(s) 228, 2880
BstSLI GKGCMC 1 cut(s) 1650
BstV1I GCAGC 4 cut(s) 214, 329, 433, 1444
BstV2I GAAGAC 3 cut(s) 1108, 1733, 2070
BstX2I RGATCY 2 cut(s) 958, 2231
BstYI RGATCY 2 cut(s) 958, 2231
Bsu15I ATCGAT 3 cut(s) 668, 2910, 2997
Bsu36I CCTNAGG 1 cut(s) 796
BsuI GTATCC 1 cut(s) 2132
BsuRI GGCC 7 cut(s) 1004, 1126, 1132, 1558, 2245, 2935, 3112
BsuTUI ATCGAT 3 cut(s) 668, 2910, 2997
BtgI CCRYGG 3 cut(s) 1515, 1642, 2019
BtrI CACGTC 2 cut(s) 240, 1120
BtsI GCAGTG 2 cut(s) 27, 37
BtsIMutI CAGTG 4 cut(s) 27, 37, 1800, 1890
Cac8I GCNNGC 6 cut(s) 588, 1355, 1888, 2676, 2800, 3040
CaiI CAGNNNCTG 2 cut(s) 227, 1559
CciI TCATGA 1 cut(s) 2148
CfoI GCGC 4 cut(s) 642, 951, 1734, 2443
Cfr10I RCCGGY 1 cut(s) 3112
ClaI ATCGAT 3 cut(s) 668, 2910, 2997
Csp6I GTAC 4 cut(s) 2613, 2620, 2637, 2852
CviQI GTAC 4 cut(s) 2613, 2620, 2637, 2852
DraI TTTAAA 2 cut(s) 1206, 2220
DrdI GACNNNNNNGTC 1 cut(s) 1539
DseDI GACNNNNNNGTC 1 cut(s) 1539
Eam1104I CTCTTC 5 cut(s) 149, 1689, 1769, 2234, 2595
EarI CTCTTC 5 cut(s) 149, 1689, 1769, 2234, 2595
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 3 cut(s) 578, 1515, 2766
Eco24I GRGCYC 1 cut(s) 1680
Eco32I GATATC 1 cut(s) 747
Eco47I GGWCC 8 cut(s) 453, 545, 716, 792, 1179, 1868, 2024, 3099
Eco47III AGCGCT 1 cut(s) 950
Eco57I CTGAAG 3 cut(s) 2247, 2325, 2538
Eco81I CCTNAGG 1 cut(s) 796
Eco88I CYCGRG 1 cut(s) 1277
EcoO109I RGGNCCY 3 cut(s) 453, 1556, 2024
EcoRI GAATTC 2 cut(s) 618, 1852
EcoRII CCWGG 6 cut(s) 547, 1021, 1126, 1679, 1897, 2513
EcoRV GATATC 1 cut(s) 747
EcoT14I CCWWGG 3 cut(s) 578, 1515, 2766
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 1680
ErhI CCWWGG 3 cut(s) 578, 1515, 2766
FalI AAGNNNNNCTT 6 cut(s) 779, 811, 935, 967, 1357, 1389
FaqI GGGAC 6 cut(s) 380, 466, 781, 1130, 1545, 2572
FauNDI CATATG 3 cut(s) 391, 893, 1503
FbaI TGATCA 1 cut(s) 2053
FblI GTMKAC 1 cut(s) 1370
Fnu4HI GCNGC 4 cut(s) 228, 318, 422, 1458
FriOI GRGCYC 1 cut(s) 1680
Fsp4HI GCNGC 4 cut(s) 228, 318, 422, 1458
FspBI CTAG 5 cut(s) 327, 1877, 2373, 2787, 3039
FspI TGCGCA 1 cut(s) 2442
GlaI GCGC 4 cut(s) 641, 950, 1733, 2442
GluI GCNGC 4 cut(s) 228, 318, 422, 1458
GsaI CCCAGC 1 cut(s) 1394
GsuI CTGGAG 4 cut(s) 624, 1776, 1816, 2700
HaeII RGCGCY 3 cut(s) 643, 952, 1735
HaeIII GGCC 7 cut(s) 1004, 1126, 1132, 1558, 2245, 2935, 3112
HapII CCGG 3 cut(s) 450, 701, 3113
HhaI GCGC 4 cut(s) 642, 951, 1734, 2443
Hin1I GRCGYC 1 cut(s) 888
Hin6I GCGC 4 cut(s) 640, 949, 1732, 2441
HinP1I GCGC 4 cut(s) 640, 949, 1732, 2441
HincII GTYRAC 3 cut(s) 1216, 2169, 2584
HindII GTYRAC 3 cut(s) 1216, 2169, 2584
HpaII CCGG 3 cut(s) 450, 701, 3113
Hpy99I CGWCG 1 cut(s) 2256
HpyAV CCTTC 9 cut(s) 211, 396, 456, 865, 1373, 2222, 2349, 2562, 2699
HpyCH4IV ACGT 5 cut(s) 239, 888, 1119, 1463, 2251
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 233, 493, 2263, 2272
HpySE526I ACGT 5 cut(s) 239, 888, 1119, 1463, 2251
Hsp92I GRCGYC 1 cut(s) 888
HspAI GCGC 4 cut(s) 640, 949, 1732, 2441
Ksp22I TGATCA 1 cut(s) 2053
LmnI GCTCC 7 cut(s) 245, 691, 819, 946, 1492, 2006, 3091
Lsp1109I GCAGC 4 cut(s) 214, 329, 433, 1444
MaeI CTAG 5 cut(s) 327, 1877, 2373, 2787, 3039
MaeII ACGT 5 cut(s) 239, 888, 1119, 1463, 2251
MbiI CCGCTC 1 cut(s) 2625
MfeI CAATTG 1 cut(s) 1526
MflI RGATCY 2 cut(s) 958, 2231
MhlI GDGCHC 4 cut(s) 124, 1497, 1650, 1680
MlyI GAGTC 3 cut(s) 821, 1823, 2777
MmeI TCCRAC 4 cut(s) 82, 623, 797, 2758
Mph1103I ATGCAT 1 cut(s) 535
MroXI GAANNNNTTC 2 cut(s) 2533, 2694
MseI TTAA 9 cut(s) 1205, 1254, 1724, 1838, 2211, 2219, 2502, 2589, 3084
MslI CAYNNNNRTG 7 cut(s) 327, 896, 1010, 1358, 1514, 1793, 3150
MspA1I CMGCKG 1 cut(s) 1247
MspCI CTTAAG 1 cut(s) 1837
MspI CCGG 3 cut(s) 450, 701, 3113
MspR9I CCNGG 7 cut(s) 451, 549, 1023, 1128, 1681, 1899, 2515
MunI CAATTG 1 cut(s) 1526
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 6 cut(s) 549, 1023, 1128, 1681, 1899, 2515
MwoI GCNNNNNNNGC 5 cut(s) 227, 233, 493, 2263, 2272
NciI CCSGG 1 cut(s) 451
NcoI CCATGG 1 cut(s) 1515
NdeI CATATG 3 cut(s) 391, 893, 1503
NheI GCTAGC 1 cut(s) 3038
NlaIV GGNNCC 4 cut(s) 113, 454, 1869, 2025
NmuCI GTSAC 5 cut(s) 570, 884, 1261, 2431, 3160
NsbI TGCGCA 1 cut(s) 2442
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 5 cut(s) 761, 1357, 2264, 2678, 2802
NspV TTCGAA 1 cut(s) 405
OliI CACNNNNGTG 1 cut(s) 1793
PaeI GCATGC 3 cut(s) 1357, 2678, 2802
PagI TCATGA 1 cut(s) 2148
PasI CCCWGGG 1 cut(s) 1022
PciI ACATGT 2 cut(s) 757, 2260
PcsI WCGNNNNNNNCGW 1 cut(s) 3003
PctI GAATGC 1 cut(s) 413
PdmI GAANNNNTTC 2 cut(s) 2533, 2694
PflMI CCANNNNNTGG 1 cut(s) 1112
PfoI TCCNGGA 2 cut(s) 449, 547
PkrI GCNGC 4 cut(s) 229, 319, 423, 1459
PleI GAGTC 3 cut(s) 821, 1823, 2777
PpsI GAGTC 3 cut(s) 821, 1823, 2777
PpuMI RGGWCCY 2 cut(s) 453, 2024
PscI ACATGT 2 cut(s) 757, 2260
PshBI ATTAAT 2 cut(s) 1724, 2589
Psp5II RGGWCCY 2 cut(s) 453, 2024
Psp6I CCWGG 6 cut(s) 547, 1021, 1126, 1679, 1897, 2513
PspFI CCCAGC 1 cut(s) 1390
PspGI CCWGG 6 cut(s) 547, 1021, 1126, 1679, 1897, 2513
PspN4I GGNNCC 4 cut(s) 113, 454, 1869, 2025
PspPPI RGGWCCY 2 cut(s) 453, 2024
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 2 cut(s) 227, 1559
PsuI RGATCY 2 cut(s) 958, 2231
PvuII CAGCTG 1 cut(s) 1247
RsaI GTAC 4 cut(s) 2614, 2621, 2638, 2853
RsaNI GTAC 4 cut(s) 2613, 2620, 2637, 2852
RseI CAYNNNNRTG 7 cut(s) 327, 896, 1010, 1358, 1514, 1793, 3150
SaqAI TTAA 9 cut(s) 1205, 1254, 1724, 1838, 2211, 2219, 2502, 2589, 3084
SatI GCNGC 4 cut(s) 228, 318, 422, 1458
SchI GAGTC 3 cut(s) 821, 1823, 2777
ScrFI CCNGG 7 cut(s) 451, 549, 1023, 1128, 1681, 1899, 2515
SduI GDGCHC 4 cut(s) 124, 1497, 1650, 1680
SfcI CTRYAG 2 cut(s) 228, 2880
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 8 cut(s) 453, 545, 716, 792, 1179, 1868, 2024, 3099
SmiMI CAYNNNNRTG 7 cut(s) 327, 896, 1010, 1358, 1514, 1793, 3150
SmlI CTYRAG 5 cut(s) 437, 491, 1823, 1837, 2669
SmoI CTYRAG 5 cut(s) 437, 491, 1823, 1837, 2669
SpeI ACTAGT 1 cut(s) 2372
SphI GCATGC 3 cut(s) 1357, 2678, 2802
SsiI CCGC 5 cut(s) 45, 95, 2426, 2548, 2623
SspI AATATT 2 cut(s) 282, 1288
SspMI CTAG 5 cut(s) 327, 1877, 2373, 2787, 3039
StyD4I CCNGG 7 cut(s) 449, 547, 1021, 1126, 1679, 1897, 2513
StyI CCWWGG 3 cut(s) 578, 1515, 2766
TaiI ACGT 5 cut(s) 242, 891, 1122, 1466, 2254
TaqII GACCGA 1 cut(s) 704
TatI WGTACW 2 cut(s) 2636, 2851
Tru1I TTAA 9 cut(s) 1205, 1254, 1724, 1838, 2211, 2219, 2502, 2589, 3084
Tru9I TTAA 9 cut(s) 1205, 1254, 1724, 1838, 2211, 2219, 2502, 2589, 3084
TscAI CASTG 4 cut(s) 27, 37, 1800, 1897
TseFI GTSAC 5 cut(s) 570, 884, 1261, 2431, 3160
TseI GCWGC 4 cut(s) 227, 317, 421, 1457
Tsp45I GTSAC 5 cut(s) 570, 884, 1261, 2431, 3160
TspGWI ACGGA 5 cut(s) 78, 107, 542, 1233, 2677
TspRI CASTG 4 cut(s) 27, 37, 1800, 1897
Van91I CCANNNNNTGG 1 cut(s) 1112
Vha464I CTTAAG 1 cut(s) 1837
VpaK11BI GGWCC 8 cut(s) 453, 545, 716, 792, 1179, 1868, 2024, 3099
VspI ATTAAT 2 cut(s) 1724, 2589
XapI RAATTY 6 cut(s) 62, 128, 618, 1852, 1941, 2928
XceI RCATGY 5 cut(s) 761, 1357, 2264, 2678, 2802
XcmI CCANNNNNNNNNTGG 1 cut(s) 3071
XmiI GTMKAC 1 cut(s) 1370
XmnI GAANNNNTTC 2 cut(s) 2533, 2694
XspI CTAG 5 cut(s) 327, 1877, 2373, 2787, 3039
ZraI GACGTC 1 cut(s) 889
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.