Rh7DG283400

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005660
Physical Location & Seq
Forward (+)
30771539 .. 30785082
13544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG283400.1

Sequence Viewer

Length: 3183 bp
ATGGGAGAAGCACCAGTGCTCACTGCCACACTAGTAGGTGAAGCATCCTCCAAATTGACCCGATTTCCAGGTACCACTTCCATTTTTGATACCAACTCTCATCCAACCCCGATTTCGTTGCTGAGGTTGAACAAGAAGCCTGGTTCTCAGTCTGCCTTCATCAGAAAGAAGTGTACTAAGTTGGTTGCGAAAAGTTTGATATCCAAAATGGCTGGTGATGAAGGGACGCCAGCCACTGCAGATGTCACTTCAGGAGATATGATTTTCGGGCCTATTAGTGAGGATGGTGTATTCCGATTTGATTGTTCTGCAAATGATAGAGAAGCAGCACATCCTAGTCTCTCTTTTGTTAATGGCAAGGATAGGGAGACACCGATTCAGAGTCAAAAGATGCCGTCCTATATCCCAATTTTTGAACGTCTTCTTGGCCAGCAGATTGTTAAACTTGAGCTTCCTGTTGGTACCTCTTTATACGGCACTGGGGAGGTTAGCGGGCAACTTGAGCGGACAGGAAAAAGAGTTTTTACTTGGAATTCGGATGCATGGGGCTATGGTTCTGGAACTACGTCATTGTACCAGTCACATCCTTGGGTGCTAGCTGTTCTTCCTTCTGGAGAGGCATTAGGAGTTCTTGCTGATACAACAAAGCGTTGTGAGATTGATTTAAGAAAAGAATCTTTGATACAGTTCATCGCTCCATCCTCTTATCCTGTCGTTACTTTTGGTCCATTTGCTACACCCAATGCCGTTTTGAGAGCTTTATCACGTGCAATTGGTACTGTATTTATGCCCCCAAAGTGGTCCTTGGGTTATCAACAATGTCGCTGGAGCTATGACTCTGATAAGAGAGTTCAAGAGGTTACAAGAACTTTTCGGGAGAAAAAGATTCCTTGTGATGTCGTATGGATGGATATTGATTATATGGATGGTTTTCGTTGTTTCACTTTTGACAAGGACCGTTTTCAAGATCCAAAATCCTTGGTGAAGGATCTCCACCAAAATGGTTTCAAGGCAATATGGATGCTTGACCCAGGGATAAAAGTGGAAGATGGTTATTTTGTGTATGATAGTGGTTCTAAAACTGATGTCTGGATTTCGAAAGCAGATGGAAAACCTTTCGTTGGGGAGGTGTGGCCTGGGCCTTGTGTTTTCCCAGACTTTACACAGGCAAAAGTTCGATTGTGGTGGTCCAATTTAGTCAAAGATTTCATTTGTAATGGTGTTGATGGTATATGGAATGACATGAATGAACCTGCTGTTTTTAAGGTTGTCACAAAAACAATGCCTGAGAGCAATATCCATAAGGGGGATGATGAACTGGGAGGCTGCCAGAATCACTCACACTACCACAATGTCTATGGGTTGCTGATGGCAAGATCAACTTATGAAGGGATGAAATTGAATAATGCAAAGAAACGTCCTTTTGTCCTCACAAGAGCTGGATTTATTGGTAGCCAGCGGTATGCTGCAACATGGACCGGAGACAATCTTTCAACTTGGGAACACCTTCATATGAGTATTCCCATGGTACTTCAATTGGGACTTAGTGGTCAGCCACTGTCGGGGCCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTGAGCTTTTTGGAAGATGGGTGGGTATAGGTTCTCTGTTTCCCTTTTGCCGTGGGCACTCTGAAAAGGACACCGCTGACCATGAGCCGTGGTCTTTTGGGGAAGAGTGTGAAGAAGTATGCCGCTTGGCATTGAGGAGGCGCTACCGCCTTATACCTCATTTATACACTCTCTTCTATATGGCTCATACGACGGGTACTCCAGTGGCAAGTCCTACATTTTTTGCTGATCCCAAAGATACCAGCTTAAGGAAATTGGAGACTTCTTTTTTGTTGGGTCCAGTTCTAGTGTATGCAAGCACTGCAGTTGGTCGGGGGATGGATGCTTTGCAGTGCACATTGCCCAAAGGAATCTGGATGAGTTTTGATTTTGATGATTCACATCCAGATTTACCAGCATTATATTTGCAAGGTGGAGCAATTATTCCTGTGGGTCCTCCTCATCAGCATGTTGGTGAATCTAATATATTAGATGACTTGACACTCCTTGTGGCTTTAAATGAACATGGGAAAGCTGAAGGTGTTATATATGAAGATGATGGTGATGGATATGAATTCCTGGAAGGAGGATTCTTGTTGACACACTATGTTGCTGAACTTCAATCATCAACACTTACTCTTAAAATTTCAAAAACTGAAGGATCTTGGAAGAGGCCAAACCGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATATGGGGTAAGGATGGAGAGATTCTTCAACTTTCAATGCCTTCAGAACAGGAGGTCTCACAGCTGGTTTCTACCAGTGAGAAACAGTACAGAACTCGTTTGGAAAATGCCAAGCGTATTCCAGCAGTAGAAGTGAGTTCTGCGCAGAAAGGAATAGAGCTTTCAAGGACGCCTATTGAATTGAAAGGTGGTGATTGGGTTATTAAAGTAGTGCCCTGGATTGGTGGTCGAATCATTTCCATGACTCACCTTCCTTCAGGGACACAGTGGCTTCACAGTAGGGTTGATATTAATGGTTATGAAGAATATAGTGGTACCGAGTACCGCTCTGCTGGATGTTCCGAGGAATACAATGTTATTGAACGAAATCTCGAGCATGCCGGGGAGGAGGAATCTCTCATGTTAGAAGGTGATATAGGGGGTGGATTGGTTCTCCAACGACAGCTATATATTCCAAAGAACGATCCCAAGGTTTTCCGGATCAACTCTAGCATTATAGCACGCAAAGTTGGTGCTGGTTCTGGTGGATATTCAAGACTGGTCTGCTTGAGAGTACACCCGATGTTCACCCTGTTGCACCCCTCAGAATCTTATGTTTCATTTACGGCCATTGACGGGTCAAAGCATGAAATTGGGCCCGAATCTAACGAGCAGTTCTATGAAGGGAAATTAATGCCTAATGGTGAATGGATGCTCATTGATAAATGCCTTGGCTTGGGGTTATTGAATCGGTTTGATGTCAGTCAGGTTTTCAAGTGCCTAATCCACTGGGGAACTGGTACTGTTAATTTGGAGCTGTGGTCCGAGGAGAGGCCTGTTTCAAACCAATCACCTCTTAGAATATCCCATGAGTATGAGGTGATAGATCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

1060

Amino Acids

117.94

Weight (kDa)

5.87

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 147 - 221 7.5e-16 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 262 - 591 7.5e-126 Glycosyl hydrolases family 31 TIM-barrel domain
DUF5110 PF17137 693 - 761 1.6e-12 Domain of unknown function (DUF5110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1548
Acc16I TGCGCA 1 cut(s) 2451
Acc36I ACCTGC 1 cut(s) 1261
Acc65I GGTACC 3 cut(s) 71, 461, 2621
AccB1I GGYRCC 4 cut(s) 71, 461, 2294, 2621
AccBSI CCGCTC 2 cut(s) 505, 2634
AccIII TCCGGA 1 cut(s) 2784
AciI CCGC 7 cut(s) 492, 505, 1459, 1674, 1723, 1747, 2632
AclWI GGATC 6 cut(s) 962, 996, 1823, 2248, 2765, 2795
AcoI YGGCCR 2 cut(s) 427, 2913
AcsI RAATTY 3 cut(s) 532, 2153, 2223
AcuI CTGAAG 5 cut(s) 234, 2136, 2256, 2334, 2547
AcvI CACGTG 1 cut(s) 767
AcyI GRCGYC 2 cut(s) 227, 2477
AflII CTTAAG 1 cut(s) 1846
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 6 cut(s) 67, 139, 1030, 1135, 2157, 2522
AjuI GAANNNNNNNTTGG 4 cut(s) 97, 129, 408, 440
Alw21I GWGCWC 2 cut(s) 21, 1937
Alw26I GTCTC 5 cut(s) 344, 362, 1476, 1853, 2368
Alw44I GTGCAC 1 cut(s) 1933
AlwI GGATC 6 cut(s) 962, 996, 1823, 2248, 2765, 2795
AlwNI CAGNNNCTG 2 cut(s) 236, 1558
Ama87I CYCGRG 1 cut(s) 2678
Aor13HI TCCGGA 1 cut(s) 2784
AoxI GGCC 9 cut(s) 269, 427, 1133, 1139, 1565, 2252, 2913, 2942, 3119
ApaI GGGCCC 1 cut(s) 2946
ApaLI GTGCAC 1 cut(s) 1933
ApeKI GCWGC 3 cut(s) 326, 1326, 1466
ApoI RAATTY 3 cut(s) 532, 2153, 2223
ArsI GACNNNNNNTTYG 2 cut(s) 1852, 1884
AseI ATTAAT 2 cut(s) 2598, 2978
Asp700I GAANNNNTTC 3 cut(s) 420, 1506, 2542
Asp718I GGTACC 3 cut(s) 71, 461, 2621
AspLEI GCGC 2 cut(s) 1743, 2452
AsuC2I CCSGG 1 cut(s) 2689
AsuII TTCGAA 1 cut(s) 1097
AsuNHI GCTAGC 1 cut(s) 595
AvaI CYCGRG 1 cut(s) 2678
AvaII GGWCC 8 cut(s) 725, 801, 955, 1188, 1476, 1877, 2033, 3108
BaeGI GKGCMC 4 cut(s) 1659, 1937, 2523, 2946
BaeI ACNNNNGTAYC 2 cut(s) 1788, 1821
BalI TGGCCA 1 cut(s) 429
BanI GGYRCC 4 cut(s) 71, 461, 2294, 2621
BanII GRGCYC 1 cut(s) 2946
BbrPI CACGTG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 2 cut(s) 21, 1937
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 338, 1313, 1453
BceAI ACGGC 6 cut(s) 379, 490, 731, 1635, 1672, 2928
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 6 cut(s) 69, 141, 1032, 1137, 2159, 2524
BcnI CCSGG 1 cut(s) 2689
BcoDI GTCTC 5 cut(s) 344, 362, 1476, 1853, 2368
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 5 cut(s) 32, 336, 596, 1886, 2796
BfmI CTRYAG 2 cut(s) 237, 1902
BfoI RGCGCY 1 cut(s) 1744
BfrI CTTAAG 1 cut(s) 1846
BfuAI ACCTGC 1 cut(s) 1261
BglII AGATCT 1 cut(s) 3172
BisI GCNGC 4 cut(s) 327, 1327, 1467, 1723
BlsI GCNGC 4 cut(s) 328, 1328, 1468, 1724
Bme1390I CCNGG 7 cut(s) 69, 141, 1032, 1137, 2159, 2524, 2689
Bme18I GGWCC 8 cut(s) 725, 801, 955, 1188, 1476, 1877, 2033, 3108
BmeT110I CYCGRG 1 cut(s) 2678
BmiI GGNNCC 8 cut(s) 73, 463, 1566, 1878, 2034, 2296, 2623, 2944
BmrFI CCNGG 7 cut(s) 69, 141, 1032, 1137, 2159, 2524, 2689
BmrI ACTGGG 3 cut(s) 489, 1328, 3085
BmsI GCATC 6 cut(s) 53, 381, 529, 1011, 1912, 2988
BmtI GCTAGC 1 cut(s) 599
BmuI ACTGGG 3 cut(s) 489, 1328, 3085
BpiI GAAGAC 1 cut(s) 413
BplI GAGNNNNNCTC 2 cut(s) 2618, 2650
BpmI CTGGAG 3 cut(s) 633, 847, 1785
Bpu10I CCTNAGC 2 cut(s) 122, 1602
Bpu14I TTCGAA 1 cut(s) 1097
BpuEI CTTGAG 3 cut(s) 467, 521, 2875
BpuMI CCSGG 1 cut(s) 2689
BsaAI YACGTR 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 1980
BsaHI GRCGYC 2 cut(s) 227, 2477
BsaI GGTCTC 1 cut(s) 2368
BsaWI WCCGGW 2 cut(s) 1478, 2784
BsaXI ACNNNNNCTCC 4 cut(s) 1783, 1813, 3092, 3122
Bse3DI GCAATG 1 cut(s) 1937
Bse8I GATNNNNATC 1 cut(s) 1980
BseAI TCCGGA 1 cut(s) 2784
BseBI CCWGG 6 cut(s) 69, 141, 1032, 1137, 2159, 2524
BseJI GATNNNNATC 1 cut(s) 1980
BseMI GCAATG 1 cut(s) 1937
BseMII CTCAG 5 cut(s) 113, 161, 1278, 1593, 2904
BseRI GAGGAG 4 cut(s) 1750, 2028, 2708, 3128
BseSI GKGCMC 4 cut(s) 1659, 1937, 2523, 2946
BseXI GCAGC 3 cut(s) 338, 1313, 1453
BshFI GGCC 9 cut(s) 271, 429, 1135, 1141, 1567, 2254, 2915, 2944, 3121
BshNI GGYRCC 4 cut(s) 71, 461, 2294, 2621
BsiHKAI GWGCWC 2 cut(s) 21, 1937
BsiHKCI CYCGRG 1 cut(s) 2678
BsiSI CCGG 3 cut(s) 1479, 2688, 2785
BslFI GGGAC 3 cut(s) 238, 1554, 2581
BsmAI GTCTC 5 cut(s) 344, 362, 1476, 1853, 2368
BsmFI GGGAC 3 cut(s) 238, 1554, 2581
BsnI GGCC 9 cut(s) 271, 429, 1135, 1141, 1567, 2254, 2915, 2944, 3121
Bso31I GGTCTC 1 cut(s) 2368
BsoBI CYCGRG 1 cut(s) 2678
Bsp119I TTCGAA 1 cut(s) 1097
Bsp120I GGGCCC 1 cut(s) 2942
Bsp1286I GDGCHC 5 cut(s) 21, 1659, 1937, 2523, 2946
Bsp13I TCCGGA 1 cut(s) 2784
Bsp143I GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
Bsp19I CCATGG 2 cut(s) 1524, 2298
BspACI CCGC 7 cut(s) 492, 505, 1459, 1674, 1723, 1747, 2632
BspANI GGCC 9 cut(s) 271, 429, 1135, 1141, 1567, 2254, 2915, 2944, 3121
BspCNI CTCAG 5 cut(s) 114, 160, 1279, 1594, 2903
BspEI TCCGGA 1 cut(s) 2784
BspLI GGNNCC 8 cut(s) 73, 463, 1566, 1878, 2034, 2296, 2623, 2944
BspMAI CTGCAG 2 cut(s) 241, 1906
BspMI ACCTGC 1 cut(s) 1261
BspOI GCTAGC 1 cut(s) 599
BspPI GGATC 6 cut(s) 962, 996, 1823, 2248, 2765, 2795
BspT104I TTCGAA 1 cut(s) 1097
BspT107I GGYRCC 4 cut(s) 71, 461, 2294, 2621
BspTI CTTAAG 1 cut(s) 1846
BspTNI GGTCTC 1 cut(s) 2368
BsrBI CCGCTC 2 cut(s) 505, 2634
BsrDI GCAATG 1 cut(s) 1937
BssMI GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
BssNI GRCGYC 2 cut(s) 227, 2477
BssT1I CCWWGG 7 cut(s) 587, 804, 978, 1524, 2298, 2775, 3016
Bst2UI CCWGG 6 cut(s) 69, 141, 1032, 1137, 2159, 2524
Bst4CI ACNGT 9 cut(s) 687, 781, 959, 1560, 2261, 2394, 2574, 2585, 3091
Bst6I CTCTTC 3 cut(s) 1698, 1778, 2243
BstACI GRCGYC 2 cut(s) 227, 2477
BstAFI CTTAAG 1 cut(s) 1846
BstAPI GCANNNNNTGC 2 cut(s) 1901, 2281
BstBAI YACGTR 1 cut(s) 767
BstBI TTCGAA 1 cut(s) 1097
BstC8I GCNNGC 9 cut(s) 231, 431, 494, 597, 1457, 1897, 2267, 2685, 2809
BstDEI CTNAG 8 cut(s) 122, 147, 177, 1287, 1544, 1602, 2890, 3143
BstDSI CCRYGG 4 cut(s) 1524, 1651, 1688, 2298
BstH2I RGCGCY 1 cut(s) 1744
BstHHI GCGC 2 cut(s) 1743, 2452
BstKTI GATC 8 cut(s) 970, 991, 1379, 1831, 2243, 2773, 2790, 3175
BstMAI GTCTC 5 cut(s) 344, 362, 1476, 1853, 2368
BstMBI GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
BstMWI GCNNNNNNNGC 4 cut(s) 502, 1592, 1901, 2281
BstNI CCWGG 6 cut(s) 69, 141, 1032, 1137, 2159, 2524
BstNSI RCATGY 3 cut(s) 2051, 2273, 2687
BstSCI CCNGG 7 cut(s) 67, 139, 1030, 1135, 2157, 2522, 2687
BstSFI CTRYAG 2 cut(s) 237, 1902
BstSLI GKGCMC 4 cut(s) 1659, 1937, 2523, 2946
BstV1I GCAGC 3 cut(s) 338, 1313, 1453
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 4 cut(s) 967, 988, 2240, 3172
BstXI CCANNNNNNTGG 1 cut(s) 1001
BstYI RGATCY 4 cut(s) 967, 988, 2240, 3172
BsuRI GGCC 9 cut(s) 271, 429, 1135, 1141, 1567, 2254, 2915, 2944, 3121
BtgI CCRYGG 4 cut(s) 1524, 1651, 1688, 2298
BtgZI GCGATG 1 cut(s) 676
BtsI GCAGTG 4 cut(s) 21, 234, 1899, 1937
BveI ACCTGC 1 cut(s) 1261
Cac8I GCNNGC 9 cut(s) 231, 431, 494, 597, 1457, 1897, 2267, 2685, 2809
CaiI CAGNNNCTG 2 cut(s) 236, 1558
CfoI GCGC 2 cut(s) 1743, 2452
CseI GACGC 2 cut(s) 235, 2485
DdeI CTNAG 8 cut(s) 122, 147, 177, 1287, 1544, 1602, 2890, 3143
DpnI GATC 8 cut(s) 969, 990, 1378, 1830, 2242, 2772, 2789, 3174
DpnII GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
DraI TTTAAA 1 cut(s) 2097
DrdI GACNNNNNNGTC 1 cut(s) 1548
DseDI GACNNNNNNGTC 1 cut(s) 1548
EaeI YGGCCR 2 cut(s) 427, 2913
Eam1104I CTCTTC 3 cut(s) 1698, 1778, 2243
EarI CTCTTC 3 cut(s) 1698, 1778, 2243
Eco130I CCWWGG 7 cut(s) 587, 804, 978, 1524, 2298, 2775, 3016
Eco147I AGGCCT 1 cut(s) 3121
Eco24I GRGCYC 1 cut(s) 2946
Eco31I GGTCTC 1 cut(s) 2368
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 8 cut(s) 725, 801, 955, 1188, 1476, 1877, 2033, 3108
Eco57I CTGAAG 5 cut(s) 234, 2136, 2256, 2334, 2547
Eco72I CACGTG 1 cut(s) 767
Eco88I CYCGRG 1 cut(s) 2678
EcoO109I RGGNCCY 2 cut(s) 1565, 2033
EcoRI GAATTC 2 cut(s) 532, 2153
EcoRII CCWGG 6 cut(s) 67, 139, 1030, 1135, 2157, 2522
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 7 cut(s) 587, 804, 978, 1524, 2298, 2775, 3016
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 2946
ErhI CCWWGG 7 cut(s) 587, 804, 978, 1524, 2298, 2775, 3016
FalI AAGNNNNNCTT 4 cut(s) 788, 820, 1366, 1398
FaqI GGGAC 3 cut(s) 238, 1554, 2581
FauI CCCGC 1 cut(s) 485
FauNDI CATATG 1 cut(s) 1512
Fnu4HI GCNGC 4 cut(s) 327, 1327, 1467, 1723
FriOI GRGCYC 1 cut(s) 2946
Fsp4HI GCNGC 4 cut(s) 327, 1327, 1467, 1723
FspBI CTAG 5 cut(s) 32, 336, 596, 1886, 2796
FspI TGCGCA 1 cut(s) 2451
GlaI GCGC 2 cut(s) 1742, 2451
GluI GCNGC 4 cut(s) 327, 1327, 1467, 1723
GsuI CTGGAG 3 cut(s) 633, 847, 1785
HaeII RGCGCY 1 cut(s) 1744
HaeIII GGCC 9 cut(s) 271, 429, 1135, 1141, 1567, 2254, 2915, 2944, 3121
HapII CCGG 3 cut(s) 1479, 2688, 2785
HgaI GACGC 2 cut(s) 235, 2485
HhaI GCGC 2 cut(s) 1743, 2452
Hin1I GRCGYC 2 cut(s) 227, 2477
Hin6I GCGC 2 cut(s) 1741, 2450
HinP1I GCGC 2 cut(s) 1741, 2450
HincII GTYRAC 1 cut(s) 2178
HindII GTYRAC 1 cut(s) 2178
HpaII CCGG 3 cut(s) 1479, 2688, 2785
Hpy166II GTNNAC 5 cut(s) 174, 1935, 2178, 2863, 2874
Hpy8I GTNNAC 5 cut(s) 174, 1935, 2178, 2863, 2874
Hpy99I CGWCG 2 cut(s) 1795, 2265
HpyCH4III ACNGT 9 cut(s) 687, 781, 959, 1560, 2261, 2394, 2574, 2585, 3091
HpyCH4IV ACGT 4 cut(s) 418, 566, 766, 1417
HpyF10VI GCNNNNNNNGC 4 cut(s) 502, 1592, 1901, 2281
HpyF3I CTNAG 8 cut(s) 122, 147, 177, 1287, 1544, 1602, 2890, 3143
HpySE526I ACGT 4 cut(s) 418, 566, 766, 1417
Hsp92I GRCGYC 2 cut(s) 227, 2477
HspAI GCGC 2 cut(s) 1741, 2450
Kpn2I TCCGGA 1 cut(s) 2784
KpnI GGTACC 3 cut(s) 75, 465, 2625
Kzo9I GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
LmnI GCTCC 4 cut(s) 700, 828, 2015, 3100
Lsp1109I GCAGC 3 cut(s) 338, 1313, 1453
LweI GCATC 6 cut(s) 53, 381, 529, 1011, 1912, 2988
MaeI CTAG 5 cut(s) 32, 336, 596, 1886, 2796
MaeII ACGT 4 cut(s) 418, 566, 766, 1417
MaeIII GTNAC 5 cut(s) 244, 579, 715, 859, 1270
MalI GATC 8 cut(s) 969, 990, 1378, 1830, 2242, 2772, 2789, 3174
MbiI CCGCTC 2 cut(s) 505, 2634
MboI GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
MfeI CAATTG 2 cut(s) 771, 1535
MflI RGATCY 4 cut(s) 967, 988, 2240, 3172
MhlI GDGCHC 5 cut(s) 21, 1659, 1937, 2523, 2946
MlsI TGGCCA 1 cut(s) 429
MluNI TGGCCA 1 cut(s) 429
MlyI GAGTC 3 cut(s) 391, 830, 2545
MmeI TCCRAC 2 cut(s) 128, 2767
Mox20I TGGCCA 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 544
MroI TCCGGA 1 cut(s) 2784
MroXI GAANNNNTTC 3 cut(s) 420, 1506, 2542
MscI TGGCCA 1 cut(s) 429
MslI CAYNNNNRTG 5 cut(s) 999, 2046, 2052, 2546, 3159
Msp20I TGGCCA 1 cut(s) 429
MspA1I CMGCKG 3 cut(s) 1459, 1676, 2371
MspCI CTTAAG 1 cut(s) 1846
MspI CCGG 3 cut(s) 1479, 2688, 2785
MspR9I CCNGG 7 cut(s) 69, 141, 1032, 1137, 2159, 2524, 2689
MunI CAATTG 2 cut(s) 771, 1535
MvaI CCWGG 6 cut(s) 69, 141, 1032, 1137, 2159, 2524
MwoI GCNNNNNNNGC 4 cut(s) 502, 1592, 1901, 2281
NciI CCSGG 1 cut(s) 2689
NcoI CCATGG 2 cut(s) 1524, 2298
NdeI CATATG 1 cut(s) 1512
NdeII GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
NheI GCTAGC 1 cut(s) 595
NlaIV GGNNCC 8 cut(s) 73, 463, 1566, 1878, 2034, 2296, 2623, 2944
NmuCI GTSAC 3 cut(s) 244, 579, 1270
NsbI TGCGCA 1 cut(s) 2451
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 3 cut(s) 2051, 2273, 2687
NspV TTCGAA 1 cut(s) 1097
PaeI GCATGC 1 cut(s) 2687
PaeR7I CTCGAG 1 cut(s) 2678
PasI CCCWGGG 1 cut(s) 1031
PceI AGGCCT 1 cut(s) 3121
PdmI GAANNNNTTC 3 cut(s) 420, 1506, 2542
PfoI TCCNGGA 1 cut(s) 2157
PkrI GCNGC 4 cut(s) 328, 1328, 1468, 1724
PleI GAGTC 3 cut(s) 390, 830, 2545
PmaCI CACGTG 1 cut(s) 767
PmlI CACGTG 1 cut(s) 767
PpsI GAGTC 3 cut(s) 390, 830, 2545
Ppu21I YACGTR 1 cut(s) 767
PpuMI RGGWCCY 1 cut(s) 2033
PshBI ATTAAT 2 cut(s) 2598, 2978
Psp5II RGGWCCY 1 cut(s) 2033
Psp6I CCWGG 6 cut(s) 67, 139, 1030, 1135, 2157, 2522
PspCI CACGTG 1 cut(s) 767
PspGI CCWGG 6 cut(s) 67, 139, 1030, 1135, 2157, 2522
PspN4I GGNNCC 8 cut(s) 73, 463, 1566, 1878, 2034, 2296, 2623, 2944
PspOMI GGGCCC 1 cut(s) 2942
PspPPI RGGWCCY 1 cut(s) 2033
PstI CTGCAG 2 cut(s) 241, 1906
PstNI CAGNNNCTG 2 cut(s) 236, 1558
PsuI RGATCY 4 cut(s) 967, 988, 2240, 3172
PvuII CAGCTG 1 cut(s) 2371
RseI CAYNNNNRTG 5 cut(s) 999, 2046, 2052, 2546, 3159
SatI GCNGC 4 cut(s) 327, 1327, 1467, 1723
Sau3AI GATC 8 cut(s) 967, 988, 1376, 1828, 2240, 2770, 2787, 3172
SchI GAGTC 3 cut(s) 391, 830, 2545
ScrFI CCNGG 7 cut(s) 69, 141, 1032, 1137, 2159, 2524, 2689
SduI GDGCHC 5 cut(s) 21, 1659, 1937, 2523, 2946
SfaNI GCATC 6 cut(s) 53, 381, 529, 1011, 1912, 2988
SfcI CTRYAG 2 cut(s) 237, 1902
Sfr274I CTCGAG 1 cut(s) 2678
SfuI TTCGAA 1 cut(s) 1097
SinI GGWCC 8 cut(s) 725, 801, 955, 1188, 1476, 1877, 2033, 3108
SlaI CTCGAG 1 cut(s) 2678
SmiMI CAYNNNNRTG 5 cut(s) 999, 2046, 2052, 2546, 3159
SmlI CTYRAG 5 cut(s) 446, 500, 1846, 2678, 2854
SmoI CTYRAG 5 cut(s) 446, 500, 1846, 2678, 2854
SpeI ACTAGT 1 cut(s) 31
SphI GCATGC 1 cut(s) 2687
SseBI AGGCCT 1 cut(s) 3121
SsiI CCGC 7 cut(s) 492, 505, 1459, 1674, 1723, 1747, 2632
SspMI CTAG 5 cut(s) 32, 336, 596, 1886, 2796
StuI AGGCCT 1 cut(s) 3121
StyD4I CCNGG 7 cut(s) 67, 139, 1030, 1135, 2157, 2522, 2687
StyI CCWWGG 7 cut(s) 587, 804, 978, 1524, 2298, 2775, 3016
TaaI ACNGT 9 cut(s) 687, 781, 959, 1560, 2261, 2394, 2574, 2585, 3091
TaiI ACGT 4 cut(s) 421, 569, 769, 1420
TaqI TCGA 4 cut(s) 1097, 1177, 2536, 2679
TatI WGTACW 3 cut(s) 173, 2394, 2860
TauI GCSGC 1 cut(s) 1725
TseFI GTSAC 3 cut(s) 244, 579, 1270
TseI GCWGC 3 cut(s) 326, 1326, 1466
Tsp45I GTSAC 3 cut(s) 244, 579, 1270
Vha464I CTTAAG 1 cut(s) 1846
VneI GTGCAC 1 cut(s) 1933
VpaK11BI GGWCC 8 cut(s) 725, 801, 955, 1188, 1476, 1877, 2033, 3108
VspI ATTAAT 2 cut(s) 2598, 2978
XapI RAATTY 3 cut(s) 532, 2153, 2223
XceI RCATGY 3 cut(s) 2051, 2273, 2687
XcmI CCANNNNNNNNNTGG 2 cut(s) 1355, 3080
XhoI CTCGAG 1 cut(s) 2678
XmnI GAANNNNTTC 3 cut(s) 420, 1506, 2542
XspI CTAG 5 cut(s) 32, 336, 596, 1886, 2796
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.