Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006862

Physical Location & Seq

Genomic Coordinates Forward (+)

8558060 .. 8561766

Gene Structure 3707 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G23750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGGAGGCTCCTACGCCTCTCCTCCTTCTCGTCCTCCTCACAACCATAACCTTCTTCACCACCTCCGTCGCTGATGACCAAACAGCAATGCTCGCTTTAGCTAAATCATTCAATCCTCCGCCGTCAGATTGGTCTTCCACCACCGATTTCTGCAAATGGTCCGGCGTACGATGTACCGGCGGTCGTGTCACAACCATCAGCCTCGCTGATAAATCTCTCACCGGATTCATCGCACCTGAGATCTCGACTCTTTCAGAGCTCAAATCTGTTTCTATTCAACGTAACAAACTCTCCGGTACAATCCCTTCGTTTGCTAAACTCTCGTCTCTTCAAGAGATCTATATGGATGAGAATAATTTCGTCGGAGTTGAAACCGGAGCTTTCGCCGGACTTACTAGTCTCCAGATCTTGAGTTTAAGCGATAACAACAACATTACTACTTGGAGTTTCCCATCGGAGCTCGTCGATTCGACTTCTCTCACTACGATTTATCTCGATAACACAAACATCGCCGGAGTTTTGCCTGACATTTTCGATTCCTTAGCTTCTCTTCAAAATCTCCGGTTATCTTACAACAACATCACCGGCGTTTTACCACCGTCGCTTGGAAAATCTTCGATTCAGAATCTTTGGATCAACAATCAAGACCTAGGAATGTCAGGAACGATCGAAGTTCTTTCGAGTATGACGTCCTTGTCACAAGCTTGGCTTCACAAGAATCACTTCTTTGGACCGATTCCAGATCTCTCCAAGAGCGAGAATCTCTTCGATCTACAGCTCCGGGATAATGATTTAACCGGAATAGTACCTCCGACGCTTCTTACACTCGCTAGCTTGAAGAATATTTCTCTAGATAACAATAAATTTCAAGGACCTCTTCCTTTGTTTTCACCGGAGGTTAAAGTAACAATAGACCACAACGTTTTTTGTACTACTAAAGCTGGACAAAGCTGTAGTCCTCAGGTGATGACGCTTTTAGCGGTGGCTGGAGGTTTGGGATATCCTTCAATGTTGGCTGAGTCTTGGCAAGGTGATGATGCTTGTAGTGGTTGGGCTTATGTTAGTTGTGATTCAGCTGGGAAGAATGTTGTCACGTTGAATCTTGGGAAACATGGATTCACCGGGTTTATATCTCCGGCGATTGCGAATCTTACTTCATTGAAGAGTCTTTATCTTAATGGTAACGATTTGACTGGTGTTATTCCTAAGGAGTTGACATTTATGACTAGTTTGCAGTTAATTGATGTCTCGAATAACAATCTTAGAGGGGAGATACCTAAGTTTCCGGCTACGGTGAAGTTTAGTTACAAACCGGGGAATGCTTTGTTGGGAACTAATGGTGGAGATGGTTCGAGTCCTGGAACTGGTGGTGCTAGTGGTGGTCCTGGTGGGTCTTCTGGTGGTGGTGGTAGTAAGGTTGGTGTGATTGTTGGTGTGATTGTGGCGGTTTTAGTGTTTCTTGCCATTTTAGGATTTGTGGTTTATAAATTTGTTATGAAGAGGAAGTATGGGAGGTTTAATAGGACGGATCCTGAGAAGGTTGGGAAGATTTTGGTTAGTGATGCTGTATCTAATGGTGGTAGTGGTAATGGTGGATATGCTAATGGGCATGGAGCTAATAACTTCAATGCTTTGAATAGTCCTAGTAGTGGTGACAATAGTGATCGTTTCCTTCTTGAAGGTGGAAGTGTTACCATTCCAATGGAGGTTCTTCGCCAGGTTACAAATAATTTCAGTGAGGATAACATATTGGGCAGAGGGGGTTTCGGTGTCGTGTATGCTGGAGAATTACACGATGGAACAAAGACTGCTGTTAAGAGGATGGAATGTGCAGCAATGGGTAATAAAGGAATGAGCGAGTTTCAGGCTGAGATAGCGGTACTTACTAAGGTCAGGCATAGACATTTGGTTGCTCTATTGGGTTACTGTGTGAACGGGAACGAGAGGTTGCTTGTCTATGAGTACATGCCACAGGGAAATCTTGGACAGCATTTGTTTGAGTGGAGTGAACTCGGTTACTCTCCTTTGACATGGAAACAGAGAGTGAGCATTGCTTTAGACGTGGCAAGAGGTGTGGAATATCTCCATAGCTTGGCTCAACAAAGCTTCATCCACAGAGATTTAAAGCCCTCTAACATCCTTCTAGGAGATGACATGAGAGCCAAGGTTGCTGATTTTGGATTGGTCAAGAACGCACCTGATGGTAAATACTCTGTTGAAACAAGATTAGCAGGCACATTCGGTTATCTAGCACCAGAATACGCCGCTACTGGAAGAGTAACGACGAAAGTGGATGTGTATGCATTTGGTGTGGTTCTCATGGAAATACTAACTGGAAGAAAAGCTTTAGATGATTCATTACCAGACGAGAGATCTCATCTAGTCACATGGTTCAGAAGAATCCTAATCAACAAAGAAAACATCCCAAAGGCACTCGACCAAACCTTAGAAGCAGACGAGGAAACAATGGAGAGCATTTACAGAGTTGCTGAGCTAGCTGGACACTGCACAGCCCGCGAACCTCAACAAAGACCCGACATGGGCCACGCAGTAAACGTGCTAGGTCCACTTGTAGAGAAGTGGAAACCGTCGTGCCAAGAAGAAGAAGAGAGTTTCGGAATCGATGTGAACATGAGTTTACCTCAAGCTCTACAAAGATGGCAAAACGAAGGAACATCGTCATCAACAATGTTCCATGGAGACTTCTCTTATTCTCAGACACAGTCTAGTATTCCTCCTAAAGCCTCTGGCTTTCCTAATACTTTCGATTCAGCTGATGGTCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase_Tyr

Protein Analysis

928

Amino Acids

99.96

Weight (kDa)

5.46

Isoelectric Point (pI)

41.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	24 - 59	4e-08	Leucine rich repeat N-terminal domain
LRR_8	PF13855	86 - 143	2.8e-09	Leucine rich repeat
LRR_8	PF13855	159 - 201	5.1e-06	Leucine rich repeat
LRR_8	PF13855	362 - 421	6.9e-06	Leucine rich repeat
Pkinase	PF00069	579 - 851	3.3e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	581 - 852	5.3e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23750
fragaria_vesca	FvH4_4g13110 FvH4_4g13110
malus_domestica	MD04G1019100.v1.1
prunus_persica	Prupe.1G168400_v2.0.a1 Prupe.1G168500_v2.0.a1 Prupe.1G168500_v2.0.a1
pyrus_communis	pycom04g01540 pycom13g29180
rosa_chinensis	RchiOBHm_Chr4g0412891 RchiOBHm_Chr4g0413671
rosa_laevigata	RLG00000008202 RLG00000008250
rosa_multiflora	Rmu_sc0004133.1_g000014 Rmu_sc0005621.1_g000017 Rmu_sc0012279.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357130
rosa_rugosa	Rorug04G0117300
rosa_samantha	Rh4AG176200 Rh4AG181600 Rh4BG176600 Rh4CG187200 Rh4DG173400 Rh4DG177100
rosa_wichuraiana	Rw4G014790 Rw4G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1485
AasI	GACNNNNNNGTC	2 cut(s)	396, 694
AatII	GACGTC	1 cut(s)	692
AccB7I	CCANNNNNTGG	1 cut(s)	2092
AccII	CGCG	1 cut(s)	2517
AciI	CCGC	7 cut(s)	119, 180, 980, 1445, 1877, 2265, 2515
AclI	AACGTT	1 cut(s)	921
AclWI	GGATC	3 cut(s)	641, 1523, 1536
AcsI	RAATTY	2 cut(s)	863, 1487
AcyI	GRCGYC	1 cut(s)	689
AfaI	GTAC	7 cut(s)	168, 175, 298, 807, 931, 1881, 1964
AfiI	CCNNNNNNNGG	5 cut(s)	605, 1364, 1649, 2092, 2540
AhlI	ACTAGT	2 cut(s)	395, 1226
AjiI	CACGTC	1 cut(s)	2062
AjnI	CCWGG	3 cut(s)	1357, 1384, 1716
AjuI	GAANNNNNNNTTGG	2 cut(s)	1310, 1342
Alw21I	GWGCWC	2 cut(s)	261, 462
Alw26I	GTCTC	4 cut(s)	330, 404, 1252, 2694
AlwI	GGATC	3 cut(s)	641, 1523, 1536
AoxI	GGCC	1 cut(s)	2542
ApeKI	GCWGC	1 cut(s)	1832
ApoI	RAATTY	2 cut(s)	863, 1487
ArsI	GACNNNNNNTTYG	2 cut(s)	940, 972
Asp700I	GAANNNNTTC	4 cut(s)	356, 613, 722, 764
AspA2I	CCTAGG	1 cut(s)	649
AspS9I	GGNCC	6 cut(s)	159, 731, 872, 1382, 2542, 2564
AsuC2I	CCSGG	3 cut(s)	782, 1123, 1314
AsuHPI	GGTGA	9 cut(s)	49, 211, 574, 882, 976, 1043, 1111, 1306, 1664
AsuNHI	GCTAGC	2 cut(s)	830, 2494
AvaII	GGWCC	5 cut(s)	159, 731, 872, 1382, 2564
AvrII	CCTAGG	1 cut(s)	649
AxyI	CCTNAGG	2 cut(s)	960, 1206
BaeI	ACNNNNGTAYC	2 cut(s)	1265, 1298
BamHI	GGATCC	1 cut(s)	1528
BanII	GRGCYC	2 cut(s)	261, 462
BarI	GAAGNNNNNNTAC	4 cut(s)	289, 321, 1289, 1321
BbsI	GAAGAC	2 cut(s)	126, 1386
Bbv12I	GWGCWC	2 cut(s)	261, 462
BbvI	GCAGC	1 cut(s)	1844
BccI	CCATC	8 cut(s)	203, 460, 1340, 1790, 1816, 2195, 2652, 2771
BceAI	ACGGC	1 cut(s)	106
BciT130I	CCWGG	3 cut(s)	1359, 1386, 1718
BcnI	CCSGG	3 cut(s)	782, 1123, 1314
BcoDI	GTCTC	4 cut(s)	330, 404, 1252, 2694
BcuI	ACTAGT	2 cut(s)	395, 1226
BfmI	CTRYAG	2 cut(s)	773, 952
BglII	AGATCT	5 cut(s)	240, 336, 405, 742, 2372
BisI	GCNGC	2 cut(s)	1833, 2265
BlnI	CCTAGG	1 cut(s)	649
BlpI	GCTNAGC	1 cut(s)	2490
BlsI	GCNGC	2 cut(s)	1834, 2266
Bme1390I	CCNGG	6 cut(s)	782, 1123, 1314, 1359, 1386, 1718
Bme18I	GGWCC	5 cut(s)	159, 731, 872, 1382, 2564
BmgBI	CACGTC	1 cut(s)	2062
BmgT120I	GGNCC	6 cut(s)	159, 731, 872, 1382, 2542, 2564
BmiI	GGNNCC	2 cut(s)	9, 1530
BmrFI	CCNGG	6 cut(s)	782, 1123, 1314, 1359, 1386, 1718
BmsI	GCATC	2 cut(s)	1027, 1552
BmtI	GCTAGC	2 cut(s)	834, 2498
BpiI	GAAGAC	2 cut(s)	126, 1386
BplI	GAGNNNNNCTC	2 cut(s)	2626, 2658
BpmI	CTGGAG	3 cut(s)	386, 1008, 1803
Bpu10I	CCTNAGC	1 cut(s)	541
Bpu1102I	GCTNAGC	1 cut(s)	2490
BpuEI	CTTGAG	2 cut(s)	430, 2628
BpuMI	CCSGG	3 cut(s)	782, 1123, 1314
Bsa29I	ATCGAT	1 cut(s)	2622
BsaHI	GRCGYC	1 cut(s)	689
BsaJI	CCNNGG	4 cut(s)	649, 1313, 2163, 2695
BsaWI	WCCGGW	6 cut(s)	221, 293, 374, 561, 797, 892
BsaXI	ACNNNNNCTCC	2 cut(s)	275, 305
Bsc4I	CCNNNNNNNGG	5 cut(s)	605, 1364, 1649, 2092, 2540
Bse118I	RCCGGY	2 cut(s)	176, 584
Bse1I	ACTGG	4 cut(s)	1198, 1369, 2275, 2338
Bse21I	CCTNAGG	2 cut(s)	960, 1206
Bse3DI	GCAATG	3 cut(s)	93, 1842, 2049
BseBI	CCWGG	3 cut(s)	1359, 1386, 1718
BseCI	ATCGAT	1 cut(s)	2622
BseDI	CCNNGG	4 cut(s)	649, 1313, 2163, 2695
BseGI	GGATG	6 cut(s)	352, 1827, 2109, 2136, 2299, 2421
BseLI	CCNNNNNNNGG	5 cut(s)	605, 1364, 1649, 2092, 2540
BseMI	GCAATG	3 cut(s)	93, 1842, 2049
BseMII	CTCAG	7 cut(s)	228, 974, 1008, 1524, 1860, 2481, 2729
BseNI	ACTGG	4 cut(s)	1198, 1369, 2275, 2338
BseRI	GAGGAG	2 cut(s)	11, 26
BseXI	GCAGC	1 cut(s)	1844
BseYI	CCCAGC	1 cut(s)	1076
BsgI	GTGCAG	2 cut(s)	1851, 2491
Bsh1236I	CGCG	1 cut(s)	2517
Bsh1285I	CGRYCG	2 cut(s)	184, 669
BshFI	GGCC	1 cut(s)	2544
BshVI	ATCGAT	1 cut(s)	2622
BsiEI	CGRYCG	2 cut(s)	184, 669
BsiHKAI	GWGCWC	2 cut(s)	261, 462
BsiWI	CGTACG	1 cut(s)	166
BslI	CCNNNNNNNGG	5 cut(s)	605, 1364, 1649, 2092, 2540
BsmAI	GTCTC	4 cut(s)	330, 404, 1252, 2694
BsmBI	CGTCTC	1 cut(s)	330
BsmI	GAATGC	1 cut(s)	1324
BsnI	GGCC	1 cut(s)	2544
Bsp1286I	GDGCHC	2 cut(s)	261, 462
Bsp1720I	GCTNAGC	1 cut(s)	2490
Bsp19I	CCATGG	1 cut(s)	2695
BspACI	CCGC	7 cut(s)	119, 180, 980, 1445, 1877, 2265, 2515
BspANI	GGCC	1 cut(s)	2544
BspCNI	CTCAG	7 cut(s)	229, 973, 1009, 1525, 1861, 2482, 2728
BspDI	ATCGAT	1 cut(s)	2622
BspFNI	CGCG	1 cut(s)	2517
BspLI	GGNNCC	2 cut(s)	9, 1530
BspOI	GCTAGC	2 cut(s)	834, 2498
BspPI	GGATC	3 cut(s)	641, 1523, 1536
BsrDI	GCAATG	3 cut(s)	93, 1842, 2049
BsrFI	RCCGGY	2 cut(s)	176, 584
BsrI	ACTGG	4 cut(s)	1198, 1369, 2275, 2338
BssAI	RCCGGY	2 cut(s)	176, 584
BssECI	CCNNGG	4 cut(s)	649, 1313, 2163, 2695
BssNI	GRCGYC	1 cut(s)	689
BssT1I	CCWWGG	3 cut(s)	649, 2163, 2695
Bst2UI	CCWGG	3 cut(s)	1359, 1386, 1718
Bst4CI	ACNGT	5 cut(s)	600, 1294, 1928, 2589, 2724
Bst6I	CTCTTC	8 cut(s)	333, 555, 770, 882, 1157, 1493, 2269, 2601
BstACI	GRCGYC	1 cut(s)	689
BstC8I	GCNNGC	5 cut(s)	93, 832, 2233, 2496, 2515
BstDSI	CCRYGG	1 cut(s)	2695
BstF5I	GGATG	6 cut(s)	352, 1827, 2109, 2136, 2299, 2421
BstFNI	CGCG	1 cut(s)	2517
BstMAI	GTCTC	4 cut(s)	330, 404, 1252, 2694
BstMCI	CGRYCG	2 cut(s)	184, 669
BstMWI	GCNNNNNNNGC	5 cut(s)	92, 1874, 2168, 2495, 2514
BstNI	CCWGG	3 cut(s)	1359, 1386, 1718
BstNSI	RCATGY	1 cut(s)	1969
BstSCI	CCNGG	6 cut(s)	780, 1121, 1312, 1357, 1384, 1716
BstSFI	CTRYAG	2 cut(s)	773, 952
BstUI	CGCG	1 cut(s)	2517
BstV1I	GCAGC	1 cut(s)	1844
BstV2I	GAAGAC	2 cut(s)	126, 1386
BstX2I	RGATCY	6 cut(s)	240, 336, 405, 742, 1528, 2372
BstXI	CCANNNNNNTGG	1 cut(s)	1702
BstYI	RGATCY	6 cut(s)	240, 336, 405, 742, 1528, 2372
Bsu15I	ATCGAT	1 cut(s)	2622
Bsu36I	CCTNAGG	2 cut(s)	960, 1206
BsuRI	GGCC	1 cut(s)	2544
BsuTUI	ATCGAT	1 cut(s)	2622
BtgI	CCRYGG	1 cut(s)	2695
BtgZI	GCGATG	2 cut(s)	214, 493
BtrI	CACGTC	1 cut(s)	2062
BtsCI	GGATG	6 cut(s)	352, 1827, 2109, 2136, 2299, 2421
BtsI	GCAGTG	1 cut(s)	2503
BtsIMutI	CAGTG	2 cut(s)	1741, 2503
Cac8I	GCNNGC	5 cut(s)	93, 832, 2233, 2496, 2515
Cfr10I	RCCGGY	2 cut(s)	176, 584
Cfr13I	GGNCC	6 cut(s)	159, 731, 872, 1382, 2542, 2564
ClaI	ATCGAT	1 cut(s)	2622
CseI	GACGC	2 cut(s)	823, 979
Csp6I	GTAC	7 cut(s)	167, 174, 297, 806, 930, 1880, 1963
CspCI	CAANNNNNGTGG	2 cut(s)	2055, 2090
CviQI	GTAC	7 cut(s)	167, 174, 297, 806, 930, 1880, 1963
DraI	TTTAAA	1 cut(s)	2124
DrdI	GACNNNNNNGTC	2 cut(s)	396, 694
DseDI	GACNNNNNNGTC	2 cut(s)	396, 694
Eam1104I	CTCTTC	8 cut(s)	333, 555, 770, 882, 1157, 1493, 2269, 2601
EarI	CTCTTC	8 cut(s)	333, 555, 770, 882, 1157, 1493, 2269, 2601
EciI	GGCGGA	1 cut(s)	108
Ecl136II	GAGCTC	2 cut(s)	259, 460
Eco130I	CCWWGG	3 cut(s)	649, 2163, 2695
Eco24I	GRGCYC	2 cut(s)	261, 462
Eco32I	GATATC	1 cut(s)	1001
Eco47I	GGWCC	5 cut(s)	159, 731, 872, 1382, 2564
Eco53kI	GAGCTC	2 cut(s)	259, 460
Eco81I	CCTNAGG	2 cut(s)	960, 1206
EcoICRI	GAGCTC	2 cut(s)	259, 460
EcoO109I	RGGNCCY	1 cut(s)	872
EcoRII	CCWGG	3 cut(s)	1357, 1384, 1716
EcoRV	GATATC	1 cut(s)	1001
EcoT14I	CCWWGG	3 cut(s)	649, 2163, 2695
EcoT22I	ATGCAT	1 cut(s)	2305
EcoT38I	GRGCYC	2 cut(s)	261, 462
ErhI	CCWWGG	3 cut(s)	649, 2163, 2695
Esp3I	CGTCTC	1 cut(s)	330
FalI	AAGNNNNNCTT	8 cut(s)	693, 725, 707, 739, 861, 893, 2329, 2361
FauI	CCCGC	1 cut(s)	2522
Fnu4HI	GCNGC	2 cut(s)	1833, 2265
FokI	GGATG	6 cut(s)	359, 1834, 2096, 2123, 2306, 2408
FriOI	GRGCYC	2 cut(s)	261, 462
Fsp4HI	GCNGC	2 cut(s)	1833, 2265
GluI	GCNGC	2 cut(s)	1833, 2265
GsaI	CCCAGC	1 cut(s)	1080
GsuI	CTGGAG	3 cut(s)	386, 1008, 1803
HaeIII	GGCC	1 cut(s)	2544
HgaI	GACGC	2 cut(s)	823, 979
Hin1I	GRCGYC	1 cut(s)	689
HincII	GTYRAC	1 cut(s)	1215
HindII	GTYRAC	1 cut(s)	1215
HindIII	AAGCTT	3 cut(s)	702, 2104, 2343
HphI	GGTGA	9 cut(s)	49, 211, 574, 882, 976, 1043, 1111, 1306, 1664
Hpy166II	GTNNAC	7 cut(s)	1215, 1933, 2009, 2554, 2567, 2629, 2639
Hpy188I	TCNGA	9 cut(s)	127, 256, 365, 457, 624, 813, 2396, 2618, 2718
Hpy8I	GTNNAC	7 cut(s)	1215, 1933, 2009, 2554, 2567, 2629, 2639
Hpy99I	CGWCG	7 cut(s)	71, 365, 467, 604, 817, 2287, 2593
HpyAV	CCTTC	9 cut(s)	35, 61, 315, 1014, 1531, 1673, 1682, 2150, 2663
HpyCH4III	ACNGT	5 cut(s)	600, 1294, 1928, 2589, 2724
HpyCH4IV	ACGT	6 cut(s)	280, 689, 921, 1094, 2061, 2556
HpyCH4V	TGCA	5 cut(s)	153, 1234, 1832, 2303, 2508
HpyF10VI	GCNNNNNNNGC	5 cut(s)	92, 1874, 2168, 2495, 2514
HpySE526I	ACGT	6 cut(s)	280, 689, 921, 1094, 2061, 2556
Hsp92I	GRCGYC	1 cut(s)	689
LmnI	GCTCC	5 cut(s)	13, 377, 457, 783, 1613
Lsp1109I	GCAGC	1 cut(s)	1844
LweI	GCATC	2 cut(s)	1027, 1552
MaeII	ACGT	6 cut(s)	280, 689, 921, 1094, 2061, 2556
MflI	RGATCY	6 cut(s)	240, 336, 405, 742, 1528, 2372
MhlI	GDGCHC	2 cut(s)	261, 462
MluCI	AATT	6 cut(s)	355, 863, 1239, 1487, 1729, 1787
MlyI	GAGTC	4 cut(s)	241, 1028, 1174, 1363
MmeI	TCCRAC	2 cut(s)	343, 836
Mph1103I	ATGCAT	1 cut(s)	2305
MroXI	GAANNNNTTC	4 cut(s)	356, 613, 722, 764
MseI	TTAA	8 cut(s)	416, 794, 900, 1176, 1238, 1518, 1815, 2123
MslI	CAYNNNNRTG	1 cut(s)	1700
MspA1I	CMGCKG	2 cut(s)	1076, 2774
MspR9I	CCNGG	6 cut(s)	782, 1123, 1314, 1359, 1386, 1718
Mva1269I	GAATGC	1 cut(s)	1324
MvaI	CCWGG	3 cut(s)	1359, 1386, 1718
MvnI	CGCG	1 cut(s)	2517
MwoI	GCNNNNNNNGC	5 cut(s)	92, 1874, 2168, 2495, 2514
NciI	CCSGG	3 cut(s)	782, 1123, 1314
NcoI	CCATGG	1 cut(s)	2695
NheI	GCTAGC	2 cut(s)	830, 2494
NlaIV	GGNNCC	2 cut(s)	9, 1530
NmuCI	GTSAC	5 cut(s)	187, 696, 1090, 1652, 2383
NsiI	ATGCAT	1 cut(s)	2305
NspI	RCATGY	1 cut(s)	1969
PcsI	WCGNNNNNNNCGW	2 cut(s)	686, 2553
PctI	GAATGC	1 cut(s)	1324
PdmI	GAANNNNTTC	4 cut(s)	356, 613, 722, 764
Pfl23II	CGTACG	1 cut(s)	166
PflFI	GACNNNGTC	1 cut(s)	2722
PflMI	CCANNNNNTGG	1 cut(s)	2092
PfoI	TCCNGGA	2 cut(s)	780, 1357
PkrI	GCNGC	2 cut(s)	1834, 2266
Ple19I	CGATCG	1 cut(s)	669
PleI	GAGTC	4 cut(s)	241, 1027, 1173, 1362
PpsI	GAGTC	4 cut(s)	241, 1027, 1173, 1362
PpuMI	RGGWCCY	1 cut(s)	872
PsiI	TTATAA	1 cut(s)	1485
Psp124BI	GAGCTC	2 cut(s)	261, 462
Psp1406I	AACGTT	1 cut(s)	921
Psp5II	RGGWCCY	1 cut(s)	872
Psp6I	CCWGG	3 cut(s)	1357, 1384, 1716
PspFI	CCCAGC	1 cut(s)	1076
PspGI	CCWGG	3 cut(s)	1357, 1384, 1716
PspLI	CGTACG	1 cut(s)	166
PspN4I	GGNNCC	2 cut(s)	9, 1530
PspPI	GGNCC	6 cut(s)	159, 731, 872, 1382, 2542, 2564
PspPPI	RGGWCCY	1 cut(s)	872
PsuI	RGATCY	6 cut(s)	240, 336, 405, 742, 1528, 2372
PsyI	GACNNNGTC	1 cut(s)	2722
PvuI	CGATCG	1 cut(s)	669
PvuII	CAGCTG	2 cut(s)	1076, 2774
RsaI	GTAC	7 cut(s)	168, 175, 298, 807, 931, 1881, 1964
RsaNI	GTAC	7 cut(s)	167, 174, 297, 806, 930, 1880, 1963
RseI	CAYNNNNRTG	1 cut(s)	1700
SacI	GAGCTC	2 cut(s)	261, 462
SaqAI	TTAA	8 cut(s)	416, 794, 900, 1176, 1238, 1518, 1815, 2123
SatI	GCNGC	2 cut(s)	1833, 2265
Sau96I	GGNCC	6 cut(s)	159, 731, 872, 1382, 2542, 2564
SchI	GAGTC	4 cut(s)	241, 1028, 1174, 1363
ScrFI	CCNGG	6 cut(s)	782, 1123, 1314, 1359, 1386, 1718
SduI	GDGCHC	2 cut(s)	261, 462
SfaNI	GCATC	2 cut(s)	1027, 1552
SfcI	CTRYAG	2 cut(s)	773, 952
SgrAI	CRCCGGYG	1 cut(s)	584
SinI	GGWCC	5 cut(s)	159, 731, 872, 1382, 2564
SmiMI	CAYNNNNRTG	1 cut(s)	1700
SmlI	CTYRAG	2 cut(s)	409, 2643
SmoI	CTYRAG	2 cut(s)	409, 2643
SpeI	ACTAGT	2 cut(s)	395, 1226
Sse9I	AATT	6 cut(s)	355, 863, 1239, 1487, 1729, 1787
SsiI	CCGC	7 cut(s)	119, 180, 980, 1445, 1877, 2265, 2515
SspI	AATATT	1 cut(s)	844
SstI	GAGCTC	2 cut(s)	261, 462
StyD4I	CCNGG	6 cut(s)	780, 1121, 1312, 1357, 1384, 1716
StyI	CCWWGG	3 cut(s)	649, 2163, 2695
TaaI	ACNGT	5 cut(s)	600, 1294, 1928, 2589, 2724
TaiI	ACGT	6 cut(s)	283, 692, 924, 1097, 2064, 2559
TaqII	GACCGA	1 cut(s)	748
TasI	AATT	6 cut(s)	355, 863, 1239, 1487, 1729, 1787
TatI	WGTACW	2 cut(s)	929, 1962
TauI	GCSGC	1 cut(s)	2267
Tru1I	TTAA	8 cut(s)	416, 794, 900, 1176, 1238, 1518, 1815, 2123
Tru9I	TTAA	8 cut(s)	416, 794, 900, 1176, 1238, 1518, 1815, 2123
TscAI	CASTG	2 cut(s)	1741, 2510
TseFI	GTSAC	5 cut(s)	187, 696, 1090, 1652, 2383
TseI	GCWGC	1 cut(s)	1832
Tsp45I	GTSAC	5 cut(s)	187, 696, 1090, 1652, 2383
TspDTI	ATGAA	5 cut(s)	217, 1146, 1511, 2098, 2346
TspGWI	ACGGA	2 cut(s)	55, 1541
TspRI	CASTG	2 cut(s)	1741, 2510
Tth111I	GACNNNGTC	1 cut(s)	2722
Van91I	CCANNNNNTGG	1 cut(s)	2092
VpaK11BI	GGWCC	5 cut(s)	159, 731, 872, 1382, 2564
XapI	RAATTY	2 cut(s)	863, 1487
XbaI	TCTAGA	1 cut(s)	850
XceI	RCATGY	1 cut(s)	1969
XmaJI	CCTAGG	1 cut(s)	649
XmnI	GAANNNNTTC	4 cut(s)	356, 613, 722, 764
ZraI	GACGTC	1 cut(s)	690
Zsp2I	ATGCAT	1 cut(s)	2305

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.