Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0006862

Physical Location & Seq

Genomic Coordinates Forward (+)

27004967 .. 27008017

Gene Structure 3051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008202

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2718 bp

ATGGGTCCTGTGAAAACCCTACAAGTTCTCTTCCTCTTTCTACCCCTCCTCCTCATCTTCACCGCCGCCGACGATGCCGCCGTCATGTCCAAGCTCCTCGAGAACCTCAAACCCAGCGGCTGGTCCTCCTCCGCATCCTACTGCACCTGGGACGGCGTCAAATGCGACTCCGCCACTTCCGAACGAGTCACCTCCATCAGCTTAGCCTCCATGTCCCTCTCCGGCTCTCTCCCTTCAAATCTCAACGAACTCACCGAGCTCACCACTCTCTCTCTCCAAAGCAACGCTCTCTCCGGCTCCTTACCTTCCCTCGCCAACCTCTCCAACCTCCAACAAGTCTACCTCGACACCAATAACTTCACTTCCATTCCCGCCGGCTGCTTCGGAGGCTTGACCGGCTTGCAGACCTTGAGCATGAGTAACAATGGCGATCTTGCCCCTTGGGAGTTCCCATCTGAGCTCACCCAGTCTACCAGCTTGGTCACTCTTCAAGCAGGTAACACCAACATGTATGGCAACATTCCTGATATTTTCGGCTCTTTTGCGAACTTGCAAAGTGTGAGATTGTCTTACAACAACCTTACTGGGCCTCTGCCCAAATCATTTTCTGGATCTGGGATTCAAAACCTCTGGATTAACAATCAGGCCTTTGGGTTATCTGGTACCGTTGAGGTTCTGTCAAACATGACTTCGTTGAATCAAGTGTGGCTGCATAAGAATCAGTTCACTGGTACGATCCCGGACCTGTCGCAATGTACTGCTCTCTTTGATCTTCAGCTTGGTGACAATGTCTTTACTGGGATGGTGCCGGCAACGTTGACGGGGTTGTCTTCTTTGCAAAATGTTTCTTTGGACAACAATAAGCTTCAGGGACCTATGCCGGAGTTTGGGTCCAAGGTGAAGGTCACTTTTGATGGTAATAGCTTTTGCAAAACCACTGCTGGGCCTTGTGATCCACAGGTCACTACGCTGCTTCAGGTTGCAGGGGCTTTCGGGTACCCGGAATTATTGGCTGAGTCTTGGACAGGAGACGATGCATGCGGAGGGTGGAGCTTCGTTGTTTGTGATGCTCAGAAGGTAGTTACAGTGAATCTGGAGAACAAGCATCTCAATGGGACTATTTCACCTGCCTTTGCCAACCTCACATCGTTGAAAACTTTGGTGTTGAAAAACAACAACTTGACGGGTCCGATACCGGCTAGTCTGACAACATTAACTCAGCTGGCGGTTTTTGATGCTTCCAATAACAATTTATATGGGGACATTCCGAAATTTCCGTCTGGGGTGAAGTTGACTACTAGTGGCAATTCATTGATTGGGACTACACCGAGCCCTGCAGGTGGCGGGAGTGCACCTCCTGGTAACAACGCAACTGCACCGGATGGGAGTCCGTCTCCATCATCCAATGGTTCTTCAGTGTCGCCCGGTATGATTGCTGTTGCTATTTTCATTGGAGTGTTGTTTGTATTCATCAAATGTTATTTGAGTAAAAAGCATAGGAAGGTTGGAACAGTGGATAATACGCTGAATGGAATTGAAATTGCTAAGGGCGACGTGACCAGTTGTGCAAATGGGTACAATGGAGTTGCAAGTGAGTTGCATAGCCAGAGCAGGGGTGACCTTCATGTTTCTGGGGGTGGAAATGTCGCCATTTCAATCCTAGTTCTTAGACAAGTGACAAACAAATTTTGTGACGATAATATATTGGGTAGAGGAGGATTTGGAGTTGTTTATAAAGGTGAGCTACATGATGGGACTAAAATTGCTGTTAAAAGGATGGAATCGGTGGCAATGGGTAATGAGGGACTGATGAATGGGTTTCAAGCAGAAATTGCAGTCCTCACTAGGGTTAGGCATAGGCATTTGGTTGCTCTGTTAGGATATTGCATTAATGGTAATGAGAGACTTCTAGTGTACGAGTACATGCCACAAGGAACGTTGACACAACATTTGTTTGATTGGCGGGAGAATGGGGTAACTCCTCTTACTTGGAAGCAGAGAGTGACAATAGCTTTGGATGTGGCAAGGGGAGTGGAATATCTACACAGCTTAGCTCAACAAAGTTTCATTCACAGAGATTTGAAACCTTCAAATATACTTCTTGGGGATGCCATGAGGGCGAAGGTTGCAGACTTTGGTTTGGTTAAAAATGCCCCCGACGGAAAGTATTCGGTTGAGACAAGAGTGGCAGGGACATTCGGGTATATTGCACCAGAATATGCAGCTACCGGCAAAGTGACAACAAAGGTGGATGTTTATGCATTTGGAGTAGTGTTGATGGAGTTGCTGAGTGGTCGAAAAGCTGTAGATGATACCATGCCAGATGAAAGGTCTCATTTGGTGTCATGGTTTCGCAGAGTCCTTGTCAACAAAGAAAACATTCCCAACTCCATCGACCAAACTCTTGACGCTGATGAGGAGACAATGGAGAGCATATACAAAGTTGCTGAGCTTGCAGAACACTGCACAGCTCCTGAGTCATATCAGAGACCAGATATGGGCCATGCAGTCAACATCTTGGGTCCTCTTGTTGAGCACTGGAAACCTACAACCCACGAAGAAGATGAAGGTAATTCAAGGATGTTTGATGATCTGTCCTATGTGCACATGAACCTTTCTCAAGCAGCGGAAAGATGGCAAGCCGATGAAGGTACTTCAAGGATGTTTGATGATCTGTCCTCTAGCCATACTCAATCAAGCATCCCTTCAAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

906

Amino Acids

97.15

Weight (kDa)

5.35

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	86 - 142	1.7e-07	Leucine rich repeat
Pkinase	PF00069	566 - 837	6.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	567 - 801	6.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23750
fragaria_vesca	FvH4_4g13110 FvH4_4g13110
malus_domestica	MD04G1019100.v1.1
prunus_persica	Prupe.1G168400_v2.0.a1 Prupe.1G168500_v2.0.a1 Prupe.1G168500_v2.0.a1
pyrus_communis	pycom04g01540 pycom13g29180
rosa_chinensis	RchiOBHm_Chr4g0412891 RchiOBHm_Chr4g0413671
rosa_laevigata	RLG00000008202 RLG00000008250
rosa_multiflora	Rmu_sc0004133.1_g000014 Rmu_sc0005621.1_g000017 Rmu_sc0012279.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357130
rosa_rugosa	Rorug04G0117300
rosa_samantha	Rh4AG176200 Rh4AG181600 Rh4BG176600 Rh4CG187200 Rh4DG173400 Rh4DG177100
rosa_wichuraiana	Rw4G014790 Rw4G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1734
AarI	CACCTGC	2 cut(s)	1135, 1328
AasI	GACNNNNNNGTC	1 cut(s)	826
Acc36I	ACCTGC	3 cut(s)	485, 1135, 1328
Acc65I	GGTACC	2 cut(s)	662, 996
AccB1I	GGYRCC	3 cut(s)	662, 805, 996
AccB7I	CCANNNNNTGG	1 cut(s)	120
AccI	GTMKAC	2 cut(s)	339, 470
AclI	AACGTT	2 cut(s)	815, 1937
AclWI	GGATC	3 cut(s)	619, 730, 947
AcsI	RAATTY	2 cut(s)	1271, 1685
AcuI	CTGAAG	4 cut(s)	758, 851, 959, 1398
AcyI	GRCGYC	1 cut(s)	156
AfaI	GTAC	8 cut(s)	664, 733, 757, 998, 1577, 1916, 1922, 2653
AfiI	CCNNNNNNNGG	7 cut(s)	120, 887, 942, 1340, 1612, 1846, 2498
AflIII	ACRYGT	1 cut(s)	507
AhlI	ACTAGT	1 cut(s)	1298
AjiI	CACGTC	1 cut(s)	1555
AjnI	CCWGG	2 cut(s)	146, 1357
Alw21I	GWGCWC	5 cut(s)	261, 462, 1354, 2538, 2607
Alw26I	GTCTC	7 cut(s)	1023, 1398, 1897, 2171, 2337, 2414, 2482
Alw44I	GTGCAC	2 cut(s)	1350, 2603
AlwI	GGATC	3 cut(s)	619, 730, 947
AlwNI	CAGNNNCTG	2 cut(s)	120, 2474
Ama87I	CYCGRG	1 cut(s)	98
AoxI	GGCC	4 cut(s)	587, 645, 944, 2500
ApaLI	GTGCAC	2 cut(s)	1350, 2603
ApeKI	GCWGC	5 cut(s)	378, 709, 970, 2222, 2624
ApoI	RAATTY	2 cut(s)	1271, 1685
ArsI	GACNNNNNNTTYG	6 cut(s)	1262, 1294, 1933, 1965, 1996, 2028
AseI	ATTAAT	1 cut(s)	1890
Asp700I	GAANNNNTTC	3 cut(s)	722, 2167, 2379
Asp718I	GGTACC	2 cut(s)	662, 996
AsuC2I	CCSGG	3 cut(s)	740, 1001, 1425
AvaI	CYCGRG	1 cut(s)	98
AvaII	GGWCC	7 cut(s)	5, 123, 742, 872, 891, 1187, 2522
BaeGI	GKGCMC	2 cut(s)	1354, 2607
BanI	GGYRCC	3 cut(s)	662, 805, 996
BanII	GRGCYC	3 cut(s)	261, 462, 1334
BbsI	GAAGAC	1 cut(s)	822
Bbv12I	GWGCWC	5 cut(s)	261, 462, 1354, 2538, 2607
BbvI	GCAGC	5 cut(s)	365, 696, 957, 2234, 2636
BceAI	ACGGC	2 cut(s)	65, 169
BciT130I	CCWGG	2 cut(s)	148, 1359
BcnI	CCSGG	3 cut(s)	740, 1001, 1425
BcoDI	GTCTC	7 cut(s)	1023, 1398, 1897, 2171, 2337, 2414, 2482
BcuI	ACTAGT	1 cut(s)	1298
BfaI	CTAG	6 cut(s)	1200, 1299, 1661, 1845, 1910, 2682
BfmI	CTRYAG	2 cut(s)	1335, 2304
BfuAI	ACCTGC	3 cut(s)	485, 1135, 1328
BglI	GCCNNNNNGGC	1 cut(s)	2117
BisI	GCNGC	8 cut(s)	66, 78, 118, 379, 710, 971, 2223, 2625
BlpI	GCTNAGC	3 cut(s)	202, 2050, 2448
BlsI	GCNGC	8 cut(s)	67, 79, 119, 380, 711, 972, 2224, 2626
Bme1390I	CCNGG	5 cut(s)	148, 740, 1001, 1359, 1425
Bme18I	GGWCC	7 cut(s)	5, 123, 742, 872, 891, 1187, 2522
BmeT110I	CYCGRG	1 cut(s)	98
BmgBI	CACGTC	1 cut(s)	1555
BmiI	GGNNCC	9 cut(s)	6, 298, 664, 807, 873, 892, 998, 1188, 2523
BmrFI	CCNGG	5 cut(s)	148, 740, 1001, 1359, 1425
BmrI	ACTGGG	3 cut(s)	460, 594, 807
BmsI	GCATC	8 cut(s)	64, 143, 1024, 1057, 1114, 1225, 2098, 2709
BmuI	ACTGGG	3 cut(s)	460, 594, 807
BpiI	GAAGAC	1 cut(s)	822
BplI	GAGNNNNNCTC	4 cut(s)	1339, 1371, 1378, 1410
BpmI	CTGGAG	1 cut(s)	1115
Bpu10I	CCTNAGC	1 cut(s)	1545
Bpu1102I	GCTNAGC	3 cut(s)	202, 2050, 2448
BpuEI	CTTGAG	2 cut(s)	430, 2604
BpuMI	CCSGG	3 cut(s)	740, 1001, 1425
BsaHI	GRCGYC	1 cut(s)	156
BsaI	GGTCTC	2 cut(s)	2337, 2482
BsaJI	CCNNGG	3 cut(s)	147, 440, 894
BsaWI	WCCGGW	1 cut(s)	1378
BsaXI	ACNNNNNCTCC	6 cut(s)	33, 63, 275, 305, 875, 905
Bsc4I	CCNNNNNNNGG	7 cut(s)	120, 887, 942, 1340, 1612, 1846, 2498
Bse118I	RCCGGY	5 cut(s)	374, 395, 808, 1195, 2228
Bse1I	ACTGG	6 cut(s)	466, 589, 733, 802, 1560, 2543
Bse3DI	GCAATG	2 cut(s)	758, 1797
BseBI	CCWGG	2 cut(s)	148, 1359
BseDI	CCNNGG	3 cut(s)	147, 440, 894
BseLI	CCNNNNNNNGG	7 cut(s)	120, 887, 942, 1340, 1612, 1846, 2498
BseMI	GCAATG	2 cut(s)	758, 1797
BseMII	CTCAG	7 cut(s)	447, 1005, 1085, 1232, 2279, 2439, 2466
BseNI	ACTGG	6 cut(s)	466, 589, 733, 802, 1560, 2543
BseRI	GAGGAG	7 cut(s)	38, 41, 86, 118, 1728, 1971, 2432
BseSI	GKGCMC	2 cut(s)	1354, 2607
BseXI	GCAGC	5 cut(s)	365, 696, 957, 2234, 2636
BseYI	CCCAGC	2 cut(s)	113, 941
BsgI	GTGCAG	3 cut(s)	127, 1359, 2449
BshFI	GGCC	4 cut(s)	589, 647, 946, 2502
BshNI	GGYRCC	3 cut(s)	662, 805, 996
BsiHKAI	GWGCWC	5 cut(s)	261, 462, 1354, 2538, 2607
BsiHKCI	CYCGRG	1 cut(s)	98
BslFI	GGGAC	9 cut(s)	164, 199, 885, 1129, 1274, 1333, 1768, 1818, 2206
BslI	CCNNNNNNNGG	7 cut(s)	120, 887, 942, 1340, 1612, 1846, 2498
BsmAI	GTCTC	7 cut(s)	1023, 1398, 1897, 2171, 2337, 2414, 2482
BsmBI	CGTCTC	2 cut(s)	1023, 1398
BsmFI	GGGAC	9 cut(s)	164, 199, 885, 1129, 1274, 1333, 1768, 1818, 2206
BsnI	GGCC	4 cut(s)	589, 647, 946, 2502
Bso31I	GGTCTC	2 cut(s)	2337, 2482
BsoBI	CYCGRG	1 cut(s)	98
Bsp1286I	GDGCHC	6 cut(s)	261, 462, 1334, 1354, 2538, 2607
Bsp143I	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
Bsp1720I	GCTNAGC	3 cut(s)	202, 2050, 2448
BspANI	GGCC	4 cut(s)	589, 647, 946, 2502
BspCNI	CTCAG	7 cut(s)	448, 1006, 1084, 1231, 2280, 2440, 2467
BspLI	GGNNCC	9 cut(s)	6, 298, 664, 807, 873, 892, 998, 1188, 2523
BspMAI	CTGCAG	1 cut(s)	1339
BspMI	ACCTGC	3 cut(s)	485, 1135, 1328
BspPI	GGATC	3 cut(s)	619, 730, 947
BspT107I	GGYRCC	3 cut(s)	662, 805, 996
BspTNI	GGTCTC	2 cut(s)	2337, 2482
BsrDI	GCAATG	2 cut(s)	758, 1797
BsrFI	RCCGGY	5 cut(s)	374, 395, 808, 1195, 2228
BsrI	ACTGG	6 cut(s)	466, 589, 733, 802, 1560, 2543
BssAI	RCCGGY	5 cut(s)	374, 395, 808, 1195, 2228
BssECI	CCNNGG	3 cut(s)	147, 440, 894
BssMI	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
BssNI	GRCGYC	1 cut(s)	156
BssT1I	CCWWGG	2 cut(s)	440, 894
Bst2UI	CCWGG	2 cut(s)	148, 1359
Bst4CI	ACNGT	3 cut(s)	667, 1087, 1513
Bst6I	CTCTTC	2 cut(s)	35, 492
BstACI	GRCGYC	1 cut(s)	156
BstAPI	GCANNNNNTGC	1 cut(s)	1832
BstC8I	GCNNGC	7 cut(s)	376, 401, 810, 1039, 1224, 2454, 2640
BstEII	GGTNACC	1 cut(s)	1616
BstKTI	GATC	7 cut(s)	433, 614, 738, 772, 955, 2593, 2674
BstMAI	GTCTC	7 cut(s)	1023, 1398, 1897, 2171, 2337, 2414, 2482
BstMBI	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 162, 387, 396, 1832, 2117, 2126, 2453
BstNI	CCWGG	2 cut(s)	148, 1359
BstNSI	RCATGY	3 cut(s)	511, 1041, 1927
BstPI	GGTNACC	1 cut(s)	1616
BstSCI	CCNGG	5 cut(s)	146, 738, 999, 1357, 1423
BstSFI	CTRYAG	2 cut(s)	1335, 2304
BstSLI	GKGCMC	2 cut(s)	1354, 2607
BstV1I	GCAGC	5 cut(s)	365, 696, 957, 2234, 2636
BstV2I	GAAGAC	1 cut(s)	822
BstX2I	RGATCY	1 cut(s)	611
BstYI	RGATCY	1 cut(s)	611
BsuRI	GGCC	4 cut(s)	589, 647, 946, 2502
BtrI	CACGTC	1 cut(s)	1555
BtsI	GCAGTG	2 cut(s)	936, 2461
BtsIMutI	CAGTG	7 cut(s)	726, 936, 1092, 1422, 1518, 2461, 2536
BveI	ACCTGC	3 cut(s)	485, 1135, 1328
Cac8I	GCNNGC	7 cut(s)	376, 401, 810, 1039, 1224, 2454, 2640
CaiI	CAGNNNCTG	2 cut(s)	120, 2474
Cfr10I	RCCGGY	5 cut(s)	374, 395, 808, 1195, 2228
CseI	GACGC	2 cut(s)	145, 2417
Csp6I	GTAC	8 cut(s)	663, 732, 756, 997, 1576, 1915, 1921, 2652
CspCI	CAANNNNNGTGG	4 cut(s)	2013, 2048, 2229, 2264
CviQI	GTAC	8 cut(s)	663, 732, 756, 997, 1576, 1915, 1921, 2652
DpnI	GATC	7 cut(s)	432, 613, 737, 771, 954, 2592, 2673
DpnII	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
DrdI	GACNNNNNNGTC	1 cut(s)	826
DseDI	GACNNNNNNGTC	1 cut(s)	826
Eam1104I	CTCTTC	2 cut(s)	35, 492
EarI	CTCTTC	2 cut(s)	35, 492
EciI	GGCGGA	1 cut(s)	160
Ecl136II	GAGCTC	2 cut(s)	259, 460
Eco130I	CCWWGG	2 cut(s)	440, 894
Eco147I	AGGCCT	1 cut(s)	647
Eco24I	GRGCYC	3 cut(s)	261, 462, 1334
Eco31I	GGTCTC	2 cut(s)	2337, 2482
Eco47I	GGWCC	7 cut(s)	5, 123, 742, 872, 891, 1187, 2522
Eco53kI	GAGCTC	2 cut(s)	259, 460
Eco57I	CTGAAG	4 cut(s)	758, 851, 959, 1398
Eco88I	CYCGRG	1 cut(s)	98
Eco91I	GGTNACC	1 cut(s)	1616
EcoICRI	GAGCTC	2 cut(s)	259, 460
EcoO109I	RGGNCCY	3 cut(s)	5, 872, 2522
EcoO65I	GGTNACC	1 cut(s)	1616
EcoRII	CCWGG	2 cut(s)	146, 1357
EcoT14I	CCWWGG	2 cut(s)	440, 894
EcoT22I	ATGCAT	2 cut(s)	1039, 2263
EcoT38I	GRGCYC	3 cut(s)	261, 462, 1334
ErhI	CCWWGG	2 cut(s)	440, 894
Esp3I	CGTCTC	2 cut(s)	1023, 1398
FalI	AAGNNNNNCTT	1 cut(s)	2689
FaqI	GGGAC	9 cut(s)	164, 199, 885, 1129, 1274, 1333, 1768, 1818, 2206
FauI	CCCGC	3 cut(s)	379, 1337, 1956
FblI	GTMKAC	2 cut(s)	339, 470
Fnu4HI	GCNGC	8 cut(s)	66, 78, 118, 379, 710, 971, 2223, 2625
FriOI	GRGCYC	3 cut(s)	261, 462, 1334
Fsp4HI	GCNGC	8 cut(s)	66, 78, 118, 379, 710, 971, 2223, 2625
FspBI	CTAG	6 cut(s)	1200, 1299, 1661, 1845, 1910, 2682
GluI	GCNGC	8 cut(s)	66, 78, 118, 379, 710, 971, 2223, 2625
GsaI	CCCAGC	2 cut(s)	117, 945
GsuI	CTGGAG	1 cut(s)	1115
HaeIII	GGCC	4 cut(s)	589, 647, 946, 2502
HgaI	GACGC	2 cut(s)	145, 2417
Hin1I	GRCGYC	1 cut(s)	156
HincII	GTYRAC	5 cut(s)	819, 1293, 1941, 2368, 2512
HindII	GTYRAC	5 cut(s)	819, 1293, 1941, 2368, 2512
HindIII	AAGCTT	1 cut(s)	863
Hpy188I	TCNGA	8 cut(s)	181, 386, 457, 1074, 1191, 1206, 1269, 2487
Hpy188III	TCNNGA	7 cut(s)	100, 524, 609, 631, 1094, 2405, 2474
Hpy99I	CGWCG	3 cut(s)	74, 1556, 2162
HpyCH4III	ACNGT	3 cut(s)	667, 1087, 1513
HpyCH4IV	ACGT	3 cut(s)	815, 1554, 1937
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 162, 387, 396, 1832, 2117, 2126, 2453
HpySE526I	ACGT	3 cut(s)	815, 1554, 1937
Hsp92I	GRCGYC	1 cut(s)	156
KpnI	GGTACC	2 cut(s)	666, 1000
KroI	GCCGGC	2 cut(s)	374, 808
KroNI	GCCGGC	2 cut(s)	376, 810
Kzo9I	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
LmnI	GCTCC	4 cut(s)	99, 302, 1050, 2476
Lsp1109I	GCAGC	5 cut(s)	365, 696, 957, 2234, 2636
LweI	GCATC	8 cut(s)	64, 143, 1024, 1057, 1114, 1225, 2098, 2709
MaeI	CTAG	6 cut(s)	1200, 1299, 1661, 1845, 1910, 2682
MaeII	ACGT	3 cut(s)	815, 1554, 1937
MalI	GATC	7 cut(s)	432, 613, 737, 771, 954, 2592, 2673
MboI	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
MboII	GAAGA	8 cut(s)	22, 49, 479, 764, 822, 1404, 2570, 2573
MflI	RGATCY	1 cut(s)	611
MhlI	GDGCHC	6 cut(s)	261, 462, 1334, 1354, 2538, 2607
MlyI	GAGTC	6 cut(s)	161, 195, 1025, 1396, 2367, 2486
MmeI	TCCRAC	3 cut(s)	348, 355, 1486
Mph1103I	ATGCAT	2 cut(s)	1039, 2263
MroNI	GCCGGC	2 cut(s)	374, 808
MroXI	GAANNNNTTC	3 cut(s)	722, 2167, 2379
MseI	TTAA	5 cut(s)	636, 1214, 1770, 1890, 2145
MslI	CAYNNNNRTG	3 cut(s)	506, 1110, 1454
MspA1I	CMGCKG	3 cut(s)	117, 1222, 2627
MspR9I	CCNGG	5 cut(s)	148, 740, 1001, 1359, 1425
MvaI	CCWGG	2 cut(s)	148, 1359
MwoI	GCNNNNNNNGC	8 cut(s)	74, 162, 387, 396, 1832, 2117, 2126, 2453
NaeI	GCCGGC	2 cut(s)	376, 810
NciI	CCSGG	3 cut(s)	740, 1001, 1425
NdeII	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
NgoMIV	GCCGGC	2 cut(s)	374, 808
NlaIV	GGNNCC	9 cut(s)	6, 298, 664, 807, 873, 892, 998, 1188, 2523
NsiI	ATGCAT	2 cut(s)	1039, 2263
NspI	RCATGY	3 cut(s)	511, 1041, 1927
PaeI	GCATGC	1 cut(s)	1041
PaeR7I	CTCGAG	1 cut(s)	98
PaqCI	CACCTGC	2 cut(s)	1135, 1328
PceI	AGGCCT	1 cut(s)	647
PciI	ACATGT	1 cut(s)	507
PcsI	WCGNNNNNNNCGW	2 cut(s)	78, 252
PdiI	GCCGGC	2 cut(s)	376, 810
PdmI	GAANNNNTTC	3 cut(s)	722, 2167, 2379
PfeI	GAWTC	5 cut(s)	619, 697, 718, 1090, 1781
PflFI	GACNNNGTC	2 cut(s)	155, 788
PflMI	CCANNNNNTGG	1 cut(s)	120
PfoI	TCCNGGA	1 cut(s)	738
PkrI	GCNGC	8 cut(s)	67, 79, 119, 380, 711, 972, 2224, 2626
PleI	GAGTC	6 cut(s)	161, 194, 1024, 1395, 2366, 2485
PpsI	GAGTC	6 cut(s)	161, 194, 1024, 1395, 2366, 2485
PpuMI	RGGWCCY	3 cut(s)	5, 872, 2522
PscI	ACATGT	1 cut(s)	507
PshBI	ATTAAT	1 cut(s)	1890
PsiI	TTATAA	1 cut(s)	1734
Psp124BI	GAGCTC	2 cut(s)	261, 462
Psp1406I	AACGTT	2 cut(s)	815, 1937
Psp5II	RGGWCCY	3 cut(s)	5, 872, 2522
Psp6I	CCWGG	2 cut(s)	146, 1357
PspEI	GGTNACC	1 cut(s)	1616
PspFI	CCCAGC	2 cut(s)	113, 941
PspGI	CCWGG	2 cut(s)	146, 1357
PspN4I	GGNNCC	9 cut(s)	6, 298, 664, 807, 873, 892, 998, 1188, 2523
PspPPI	RGGWCCY	3 cut(s)	5, 872, 2522
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	2 cut(s)	120, 2474
PsuI	RGATCY	1 cut(s)	611
PsyI	GACNNNGTC	2 cut(s)	155, 788
PvuII	CAGCTG	1 cut(s)	1222
RsaI	GTAC	8 cut(s)	664, 733, 757, 998, 1577, 1916, 1922, 2653
RsaNI	GTAC	8 cut(s)	663, 732, 756, 997, 1576, 1915, 1921, 2652
RseI	CAYNNNNRTG	3 cut(s)	506, 1110, 1454
SacI	GAGCTC	2 cut(s)	261, 462
SaqAI	TTAA	5 cut(s)	636, 1214, 1770, 1890, 2145
SatI	GCNGC	8 cut(s)	66, 78, 118, 379, 710, 971, 2223, 2625
Sau3AI	GATC	7 cut(s)	430, 611, 735, 769, 952, 2590, 2671
SbfI	CCTGCAGG	1 cut(s)	1339
SchI	GAGTC	6 cut(s)	161, 195, 1025, 1396, 2367, 2486
ScrFI	CCNGG	5 cut(s)	148, 740, 1001, 1359, 1425
SdaI	CCTGCAGG	1 cut(s)	1339
SduI	GDGCHC	6 cut(s)	261, 462, 1334, 1354, 2538, 2607
SfaNI	GCATC	8 cut(s)	64, 143, 1024, 1057, 1114, 1225, 2098, 2709
SfcI	CTRYAG	2 cut(s)	1335, 2304
Sfr274I	CTCGAG	1 cut(s)	98
SinI	GGWCC	7 cut(s)	5, 123, 742, 872, 891, 1187, 2522
SlaI	CTCGAG	1 cut(s)	98
SmiMI	CAYNNNNRTG	3 cut(s)	506, 1110, 1454
SmlI	CTYRAG	3 cut(s)	98, 409, 2619
SmoI	CTYRAG	3 cut(s)	98, 409, 2619
SpeI	ACTAGT	1 cut(s)	1298
SphI	GCATGC	1 cut(s)	1041
Sse8387I	CCTGCAGG	1 cut(s)	1339
SseBI	AGGCCT	1 cut(s)	647
SspMI	CTAG	6 cut(s)	1200, 1299, 1661, 1845, 1910, 2682
SstI	GAGCTC	2 cut(s)	261, 462
StuI	AGGCCT	1 cut(s)	647
StyD4I	CCNGG	5 cut(s)	146, 738, 999, 1357, 1423
StyI	CCWWGG	2 cut(s)	440, 894
TaaI	ACNGT	3 cut(s)	667, 1087, 1513
TaiI	ACGT	3 cut(s)	818, 1557, 1940
TaqI	TCGA	4 cut(s)	99, 345, 2296, 2394
TatI	WGTACW	2 cut(s)	755, 1920
TauI	GCSGC	3 cut(s)	68, 80, 120
TfiI	GAWTC	5 cut(s)	619, 697, 718, 1090, 1781
Tru1I	TTAA	5 cut(s)	636, 1214, 1770, 1890, 2145
Tru9I	TTAA	5 cut(s)	636, 1214, 1770, 1890, 2145
TscAI	CASTG	7 cut(s)	733, 943, 1092, 1422, 1518, 2468, 2543
TseI	GCWGC	5 cut(s)	378, 709, 970, 2222, 2624
TspGWI	ACGGA	3 cut(s)	1266, 1380, 2175
TspRI	CASTG	7 cut(s)	733, 943, 1092, 1422, 1518, 2468, 2543
Tth111I	GACNNNGTC	2 cut(s)	155, 788
Van91I	CCANNNNNTGG	1 cut(s)	120
VneI	GTGCAC	2 cut(s)	1350, 2603
VpaK11BI	GGWCC	7 cut(s)	5, 123, 742, 872, 891, 1187, 2522
VspI	ATTAAT	1 cut(s)	1890
XapI	RAATTY	2 cut(s)	1271, 1685
XceI	RCATGY	3 cut(s)	511, 1041, 1927
XhoI	CTCGAG	1 cut(s)	98
XmiI	GTMKAC	2 cut(s)	339, 470
XmnI	GAANNNNTTC	3 cut(s)	722, 2167, 2379
XspI	CTAG	6 cut(s)	1200, 1299, 1661, 1845, 1910, 2682
Zsp2I	ATGCAT	2 cut(s)	1039, 2263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.