Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006862

Physical Location & Seq

Genomic Coordinates Reverse (-)

2205784 .. 2209451

Gene Structure 3668 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1019100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2712 bp

ATGGGTAAGCAGAAAATCCTACAAGCTCTCATTCTAGCTCTCTCTCTCCTTCGCCTGATCGCCACCGCCCCCACCGCCGCCGACGACTCCGACGTCATGTCAAAACTCACAGCAAGCCTCAACCCCGCACCCAAAGGCTGGTCAACAAGCACCGCCTACTGCAGCTGGGACGGTGTCAACTGCGACACTTCAAAGAGAGTCACCTCCATCAACTTAGCCTCCAAAGGTCTCTCCGGCTCTCTCCCTTCAAATCTCAACTCTCTCTCCCAGCTCACCACTCTCTCTCTCCAGAGCAACTCTCTCTCCGGCGCCTTTCCTTCTTTGGCCAACCTCTCTTCCCTCCAAGAAGTCTACCTCGACACCAACAACTTCACCTCCATTCCCGCCGGCTGCTTCACGGGCCTCTCGAGCTTGCAGATTTTGAGCATGAGCCAGAACATCAATCTCGACTCTTGGGTTTTCCCCTCGGAGCTCACTGAAGCTTCCAGCTTGGTCACTTTCGCTGCAGGTAATGTGAATTTATATGGGTCGTTGCCTGATGTGTTCGATTCGTTTCCGAACTTGCAAAACCTGAGGCTTTCTTACAATAACTTTACTGGTCCTTTGCCCAAAACGTTTTCTGGATCTGGGATTCAGAACCTCTGGCTTAACAATCAGCTACTTGGGTTGTCTGGCTCCATTGAAGTCTTAGCAAACATGACCCAGTTGTCCCAGGTTTGGCTTCAAAACAACCAATTCACTGGTCCGATCCCAGATCTCTCAAAGTGTGTAACTTTGTTCGATCTTCAGCTCCGCGATAACCAGTTTACGGGCATTGTGCCCGCCACATTGATGTCCATATCTTCTTTGAAGAACGTTTCTTTGGACAACAACAAGCTGCAGGGTCCAATGCCGGCTTTCAGGTCCACCGTGGAGAAATCCACAGTTGATGGTAATAGCTTTTGTCAAACGACTCCAGGGACTTGTGATCCGCTGGTCACCACGCTGCTTGAGGTTGCAGGGGCTTTCGGATATCCGGTTTTGCTTGCTGATTCTTGGACAGGAAACAAGCCTTGCAGTGGATGGAGCTTCATTGTTTGTGATCCACAGGGAAAGGTTTTCACAGTGAATTTTGACAACAAGCATTTCAATGGGACCATTTCGCCTGCTTTTGCCAATCTGACATCTTTGAAAAATTTGGTGTTGAGTCGTAACAATTTGGTTGGGTCGATACCGGATAGCTTGTTAAGCTTGACTCAGCTTCAGCTTCTTGATGTTTCCAACAACAATCTAAGTGGGGTAATTCCGAAATTTCCATCTACGGTGAAGTTAGTTACAACTGGTAATGTGTTGATTGGGACAACTCCGAGCACTGGAGGTGGAGGAGGTACTCCGTCGGGTACTGGTTCTAATGGAACTACTCCTAGTGGGAGTCCGGCTCAGGCATCAAATGGTCCTTCGGTATCCCCTGGTATGATTGCTGGTATAGTTATTGCCGTTGTCATTTTCATTGTGGTAGTATTGTTTGTGTCCTACAAATGTTATGCTGGTAAAAAGCATAAGAAATTTGGAAGGGTGGCTAATCCATTAACTGGGATAGAAATTGCTAAGAGTGATGTTATGAGTAGCGCAAATGGGTACAATGGAGTTACAAGTGAACTGCAAAGCCAGAGCAGTGGTGACCTTCATGTTTTTGAGGGTGGAAATGTTGCGATTTCGATCCAAGTTCTAAGACAAGTGACAAACAATTTTAGCGAGGACAACATATTAGGCAGAGGAGGATTTGGAATTGTTTATAAAGGCGAATTGCATGATGGGACTAAAATTGCCGTCAAACGGATGGAATCTGTGGCCGTAGGCACTAAGGGATTGAACGAGTTTCAGGCAGAAATTGCGGTCCTTACTAAGGTCAGGCATAGGCATTTGGTTGCGCTTTTGGGATCCTGTATCAATGGCAATGAGAGACTTTTGGTCTATGAGTACATGCCACAAGGGACTTTAACACAGCATTTATTTGAATGGCGCGAGATTGGTGTGCCTCCACTGACTTGGAAGCAGAGAGTAACAATAGCATTGGATGTGGCACGAGGAGTGGAATATTTGCACAGCTTGGCTCAACAAAGTTTCATTCACCGAGATTTAAAGCCCTCAAACATACTTCTGGGCGATGACATGAGGGCCAAGGTTGCTGACTTCGGACTGGTTAAAAACGCGCCTGACGGGAAGTATTCGGTTGAGACAAGGTTGGCAGGGACATTCGGGTATCTCGCACCAGAATATGCAGCTACTGGCAGAGTCACTACAAAAGTAGATGTTTATGCGTTCGGAACTCATTTGGTCTCATGGTTCCGCAGAGTCCTTGTCAACAAAGAAAACATTCCGAAGGCTATTGACCAAACTCTTGACCCCGATGAGGAGACAATGGAGAGCATATACAAAGTTGCTGAGCTGGCAGGACACTGCACTGCTCGTGAGCCGTACCAGAGACCAGACATGGGACATGCTGTCAACATCCTGGGTCCTCTTGTTGAGCACTGGAAACCTACCACCCATGAAGAAGAAAACTCTGGCATCGACCTGCACATGAGCCTTCCGCAAGCGTTGCAAAGATGGCAAGCCAATGAAGGTACTTCTAGGATGTTTGATGATATGTCCTTCTCTCAAACTCAATCAAGCATCCCTTCAAAACCTTCCGGATTTGCTGACTCATTTGATTCAATGGATTGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase_Tyr

Protein Analysis

904

Amino Acids

97.14

Weight (kDa)

6.14

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	113 - 150	6.8e-07	Leucine rich repeat
Pkinase	PF00069	578 - 829	2e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	580 - 830	4e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23750
fragaria_vesca	FvH4_4g13110 FvH4_4g13110
malus_domestica	MD04G1019100.v1.1
prunus_persica	Prupe.1G168400_v2.0.a1 Prupe.1G168500_v2.0.a1 Prupe.1G168500_v2.0.a1
pyrus_communis	pycom04g01540 pycom13g29180
rosa_chinensis	RchiOBHm_Chr4g0412891 RchiOBHm_Chr4g0413671
rosa_laevigata	RLG00000008202 RLG00000008250
rosa_multiflora	Rmu_sc0004133.1_g000014 Rmu_sc0005621.1_g000017 Rmu_sc0012279.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357130
rosa_rugosa	Rorug04G0117300
rosa_samantha	Rh4AG176200 Rh4AG181600 Rh4BG176600 Rh4CG187200 Rh4DG173400 Rh4DG177100
rosa_wichuraiana	Rw4G014790 Rw4G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1776
AasI	GACNNNNNNGTC	2 cut(s)	92, 706
AatII	GACGTC	1 cut(s)	96
Acc36I	ACCTGC	2 cut(s)	497, 2565
AccB1I	GGYRCC	1 cut(s)	308
AccB7I	CCANNNNNTGG	2 cut(s)	138, 1571
AccI	GTMKAC	1 cut(s)	351
AccII	CGCG	3 cut(s)	795, 2004, 2192
AccIII	TCCGGA	1 cut(s)	2672
AclI	AACGTT	2 cut(s)	614, 855
AclWI	GGATC	7 cut(s)	631, 742, 962, 1076, 1693, 1914, 1927
AcoI	YGGCCR	2 cut(s)	324, 1830
AcsI	RAATTY	5 cut(s)	517, 1108, 1174, 1289, 1544
AcuI	CTGAAG	3 cut(s)	498, 770, 1226
AcyI	GRCGYC	2 cut(s)	93, 309
AfaI	GTAC	6 cut(s)	1369, 1381, 1619, 1961, 2459, 2608
AhdI	GACNNNNNGTC	3 cut(s)	97, 1949, 2483
AjnI	CCWGG	4 cut(s)	711, 955, 1447, 2493
AjuI	GAANNNNNNNTTGG	2 cut(s)	845, 877
Alw21I	GWGCWC	3 cut(s)	474, 1352, 2514
Alw26I	GTCTC	6 cut(s)	233, 1936, 2210, 2323, 2390, 2458
AlwI	GGATC	7 cut(s)	631, 742, 962, 1076, 1693, 1914, 1927
AlwNI	CAGNNNCTG	1 cut(s)	2267
Ama87I	CYCGRG	1 cut(s)	406
Aor13HI	TCCGGA	1 cut(s)	2672
AoxI	GGCC	4 cut(s)	324, 400, 1830, 2157
ApeKI	GCWGC	6 cut(s)	162, 390, 503, 877, 985, 2261
ApoI	RAATTY	5 cut(s)	517, 1108, 1174, 1289, 1544
Asp700I	GAANNNNTTC	1 cut(s)	2355
AspLEI	GCGC	5 cut(s)	311, 1610, 1912, 2004, 2194
AsuHPI	GGTGA	7 cut(s)	193, 265, 364, 970, 1315, 1670, 2102
AvaI	CYCGRG	1 cut(s)	406
AvaII	GGWCC	8 cut(s)	599, 743, 884, 903, 1134, 1433, 1876, 2498
AxyI	CCTNAGG	1 cut(s)	572
BaeGI	GKGCMC	1 cut(s)	822
BalI	TGGCCA	1 cut(s)	326
BamHI	GGATCC	1 cut(s)	1919
BanI	GGYRCC	1 cut(s)	308
BanII	GRGCYC	1 cut(s)	474
BauI	CACGAG	2 cut(s)	2064, 2448
Bbv12I	GWGCWC	3 cut(s)	474, 1352, 2514
BbvI	GCAGC	6 cut(s)	174, 377, 490, 864, 972, 2273
BccI	CCATC	7 cut(s)	215, 923, 1056, 1303, 1787, 1813, 2583
BceAI	ACGGC	4 cut(s)	1460, 1793, 1817, 2440
BciT130I	CCWGG	4 cut(s)	713, 957, 1449, 2495
BciVI	GTATCC	1 cut(s)	1453
BcoDI	GTCTC	6 cut(s)	233, 1936, 2210, 2323, 2390, 2458
BfaI	CTAG	3 cut(s)	35, 1404, 2613
BfmI	CTRYAG	3 cut(s)	160, 504, 878
BfoI	RGCGCY	1 cut(s)	312
BfuAI	ACCTGC	2 cut(s)	497, 2565
BfuI	GTATCC	1 cut(s)	1453
BglII	AGATCT	1 cut(s)	754
BisI	GCNGC	7 cut(s)	78, 163, 391, 504, 878, 986, 2262
BlpI	GCTNAGC	1 cut(s)	2424
BlsI	GCNGC	7 cut(s)	79, 164, 392, 505, 879, 987, 2263
Bme1390I	CCNGG	4 cut(s)	713, 957, 1449, 2495
Bme18I	GGWCC	8 cut(s)	599, 743, 884, 903, 1134, 1433, 1876, 2498
BmeRI	GACNNNNNGTC	3 cut(s)	97, 1949, 2483
BmeT110I	CYCGRG	1 cut(s)	406
BmiI	GGNNCC	7 cut(s)	310, 676, 885, 1135, 1921, 2327, 2499
BmrFI	CCNGG	4 cut(s)	713, 957, 1449, 2495
BmrI	ACTGGG	2 cut(s)	697, 1581
BmsI	GCATC	3 cut(s)	1433, 2559, 2664
BmuI	ACTGGG	2 cut(s)	697, 1581
BplI	GAGNNNNNCTC	4 cut(s)	283, 315, 1402, 1434
BpmI	CTGGAG	3 cut(s)	272, 939, 1374
Bpu10I	CCTNAGC	1 cut(s)	1419
Bpu1102I	GCTNAGC	1 cut(s)	2424
BpuEI	CTTGAG	1 cut(s)	1010
BsaBI	GATNNNNATC	1 cut(s)	1697
BsaHI	GRCGYC	2 cut(s)	93, 309
BsaI	GGTCTC	3 cut(s)	233, 2323, 2458
BsaJI	CCNNGG	7 cut(s)	465, 711, 909, 956, 1447, 2160, 2494
BsaWI	WCCGGW	3 cut(s)	1015, 1213, 2672
BsaXI	ACNNNNNCTCC	8 cut(s)	203, 233, 248, 278, 287, 317, 359, 389
Bse118I	RCCGGY	2 cut(s)	386, 892
Bse21I	CCTNAGG	1 cut(s)	572
Bse3DI	GCAATG	1 cut(s)	1942
Bse8I	GATNNNNATC	1 cut(s)	1697
BseAI	TCCGGA	1 cut(s)	2672
BseBI	CCWGG	4 cut(s)	713, 957, 1449, 2495
BseDI	CCNNGG	7 cut(s)	465, 711, 909, 956, 1447, 2160, 2494
BseGI	GGATG	6 cut(s)	1067, 1824, 2062, 2490, 2622, 2655
BseJI	GATNNNNATC	1 cut(s)	1697
BseMI	GCAATG	1 cut(s)	1942
BseMII	CTCAG	4 cut(s)	563, 1250, 1433, 2415
BseRI	GAGGAG	4 cut(s)	1377, 1770, 2082, 2408
BseSI	GKGCMC	1 cut(s)	822
BseXI	GCAGC	6 cut(s)	174, 377, 490, 864, 972, 2273
BseYI	CCCAGC	2 cut(s)	165, 267
BsgI	GTGCAG	2 cut(s)	2425, 2543
Bsh1236I	CGCG	3 cut(s)	795, 2004, 2192
BshFI	GGCC	4 cut(s)	326, 402, 1832, 2159
BshNI	GGYRCC	1 cut(s)	308
BsiHKAI	GWGCWC	3 cut(s)	474, 1352, 2514
BsiHKCI	CYCGRG	1 cut(s)	406
BsiSI	CCGG	8 cut(s)	234, 306, 387, 893, 1016, 1214, 1415, 2673
BslFI	GGGAC	9 cut(s)	182, 694, 973, 1147, 1351, 1810, 1987, 2245, 2490
BsmAI	GTCTC	6 cut(s)	233, 1936, 2210, 2323, 2390, 2458
BsmFI	GGGAC	9 cut(s)	182, 694, 973, 1147, 1351, 1810, 1987, 2245, 2490
BsnI	GGCC	4 cut(s)	326, 402, 1832, 2159
Bso31I	GGTCTC	3 cut(s)	233, 2323, 2458
BsoBI	CYCGRG	1 cut(s)	406
Bsp1286I	GDGCHC	4 cut(s)	474, 822, 1352, 2514
Bsp13I	TCCGGA	1 cut(s)	2672
Bsp143I	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
Bsp1720I	GCTNAGC	1 cut(s)	2424
BspANI	GGCC	4 cut(s)	326, 402, 1832, 2159
BspCNI	CTCAG	4 cut(s)	564, 1249, 1432, 2416
BspEI	TCCGGA	1 cut(s)	2672
BspFNI	CGCG	3 cut(s)	795, 2004, 2192
BspLI	GGNNCC	7 cut(s)	310, 676, 885, 1135, 1921, 2327, 2499
BspMAI	CTGCAG	3 cut(s)	164, 508, 882
BspMI	ACCTGC	2 cut(s)	497, 2565
BspPI	GGATC	7 cut(s)	631, 742, 962, 1076, 1693, 1914, 1927
BspT107I	GGYRCC	1 cut(s)	308
BspTNI	GGTCTC	3 cut(s)	233, 2323, 2458
BsrDI	GCAATG	1 cut(s)	1942
BsrFI	RCCGGY	2 cut(s)	386, 892
BssAI	RCCGGY	2 cut(s)	386, 892
BssECI	CCNNGG	7 cut(s)	465, 711, 909, 956, 1447, 2160, 2494
BssMI	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
BssNI	GRCGYC	2 cut(s)	93, 309
BssSI	CACGAG	2 cut(s)	2064, 2448
BssT1I	CCWWGG	1 cut(s)	2160
Bst2BI	CACGAG	2 cut(s)	2064, 2448
Bst2UI	CCWGG	4 cut(s)	713, 957, 1449, 2495
Bst4CI	ACNGT	5 cut(s)	173, 910, 925, 1105, 1303
Bst6I	CTCTTC	1 cut(s)	340
BstACI	GRCGYC	2 cut(s)	93, 309
BstAPI	GCANNNNNTGC	2 cut(s)	1871, 2581
BstDSI	CCRYGG	1 cut(s)	909
BstEII	GGTNACC	2 cut(s)	976, 1658
BstENI	CCTNNNNNAGG	1 cut(s)	1883
BstF5I	GGATG	6 cut(s)	1067, 1824, 2062, 2490, 2622, 2655
BstFNI	CGCG	3 cut(s)	795, 2004, 2192
BstH2I	RGCGCY	1 cut(s)	312
BstHHI	GCGC	5 cut(s)	311, 1610, 1912, 2004, 2194
BstKTI	GATC	9 cut(s)	60, 626, 750, 757, 784, 970, 1084, 1701, 1922
BstMAI	GTCTC	6 cut(s)	233, 1936, 2210, 2323, 2390, 2458
BstMBI	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
BstMWI	GCNNNNNNNGC	9 cut(s)	74, 399, 408, 1227, 1871, 2165, 2429, 2581, 2590
BstNI	CCWGG	4 cut(s)	713, 957, 1449, 2495
BstNSI	RCATGY	2 cut(s)	1966, 2483
BstPI	GGTNACC	2 cut(s)	976, 1658
BstSCI	CCNGG	4 cut(s)	711, 955, 1447, 2493
BstSFI	CTRYAG	3 cut(s)	160, 504, 878
BstSLI	GKGCMC	1 cut(s)	822
BstUI	CGCG	3 cut(s)	795, 2004, 2192
BstV1I	GCAGC	6 cut(s)	174, 377, 490, 864, 972, 2273
BstX2I	RGATCY	3 cut(s)	623, 754, 1919
BstXI	CCANNNNNNTGG	3 cut(s)	740, 1655, 2028
BstYI	RGATCY	3 cut(s)	623, 754, 1919
Bsu36I	CCTNAGG	1 cut(s)	572
BsuI	GTATCC	1 cut(s)	1453
BsuRI	GGCC	4 cut(s)	326, 402, 1832, 2159
BtgI	CCRYGG	1 cut(s)	909
BtgZI	GCGATG	1 cut(s)	2160
BtsCI	GGATG	6 cut(s)	1067, 1824, 2062, 2490, 2622, 2655
BtsI	GCAGTG	4 cut(s)	1063, 1660, 2437, 2442
BveI	ACCTGC	2 cut(s)	497, 2565
CaiI	CAGNNNCTG	1 cut(s)	2267
CfoI	GCGC	5 cut(s)	311, 1610, 1912, 2004, 2194
Cfr10I	RCCGGY	2 cut(s)	386, 892
Csp6I	GTAC	6 cut(s)	1368, 1380, 1618, 1960, 2458, 2607
CspCI	CAANNNNNGTGG	4 cut(s)	970, 1005, 1255, 1290
CviQI	GTAC	6 cut(s)	1368, 1380, 1618, 1960, 2458, 2607
DinI	GGCGCC	1 cut(s)	310
DpnI	GATC	9 cut(s)	59, 625, 749, 756, 783, 969, 1083, 1700, 1921
DpnII	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
DraI	TTTAAA	1 cut(s)	2121
DrdI	GACNNNNNNGTC	2 cut(s)	92, 706
DriI	GACNNNNNGTC	3 cut(s)	97, 1949, 2483
DseDI	GACNNNNNNGTC	2 cut(s)	92, 706
EaeI	YGGCCR	2 cut(s)	324, 1830
Eam1104I	CTCTTC	1 cut(s)	340
Eam1105I	GACNNNNNGTC	3 cut(s)	97, 1949, 2483
EarI	CTCTTC	1 cut(s)	340
Ecl136II	GAGCTC	1 cut(s)	472
Eco130I	CCWWGG	1 cut(s)	2160
Eco24I	GRGCYC	1 cut(s)	474
Eco31I	GGTCTC	3 cut(s)	233, 2323, 2458
Eco32I	GATATC	1 cut(s)	1013
Eco47I	GGWCC	8 cut(s)	599, 743, 884, 903, 1134, 1433, 1876, 2498
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	3 cut(s)	498, 770, 1226
Eco81I	CCTNAGG	1 cut(s)	572
Eco88I	CYCGRG	1 cut(s)	406
Eco91I	GGTNACC	2 cut(s)	976, 1658
EcoICRI	GAGCTC	1 cut(s)	472
EcoNI	CCTNNNNNAGG	1 cut(s)	1883
EcoO109I	RGGNCCY	1 cut(s)	2498
EcoO65I	GGTNACC	2 cut(s)	976, 1658
EcoRII	CCWGG	4 cut(s)	711, 955, 1447, 2493
EcoRV	GATATC	1 cut(s)	1013
EcoT14I	CCWWGG	1 cut(s)	2160
EcoT38I	GRGCYC	1 cut(s)	474
EgeI	GGCGCC	1 cut(s)	310
EheI	GGCGCC	1 cut(s)	310
ErhI	CCWWGG	1 cut(s)	2160
FalI	AAGNNNNNCTT	2 cut(s)	2644, 2676
FaqI	GGGAC	9 cut(s)	182, 694, 973, 1147, 1351, 1810, 1987, 2245, 2490
FauI	CCCGC	3 cut(s)	133, 391, 829
FblI	GTMKAC	1 cut(s)	351
Fnu4HI	GCNGC	7 cut(s)	78, 163, 391, 504, 878, 986, 2262
FokI	GGATG	6 cut(s)	1074, 1831, 2069, 2477, 2629, 2642
FriOI	GRGCYC	1 cut(s)	474
Fsp4HI	GCNGC	7 cut(s)	78, 163, 391, 504, 878, 986, 2262
FspBI	CTAG	3 cut(s)	35, 1404, 2613
GlaI	GCGC	5 cut(s)	310, 1609, 1911, 2003, 2193
GluI	GCNGC	7 cut(s)	78, 163, 391, 504, 878, 986, 2262
GsaI	CCCAGC	2 cut(s)	169, 271
GsuI	CTGGAG	3 cut(s)	272, 939, 1374
HaeII	RGCGCY	1 cut(s)	312
HaeIII	GGCC	4 cut(s)	326, 402, 1832, 2159
HapII	CCGG	8 cut(s)	234, 306, 387, 893, 1016, 1214, 1415, 2673
HhaI	GCGC	5 cut(s)	311, 1610, 1912, 2004, 2194
Hin1I	GRCGYC	2 cut(s)	93, 309
Hin6I	GCGC	5 cut(s)	309, 1608, 1910, 2002, 2192
HinP1I	GCGC	5 cut(s)	309, 1608, 1910, 2002, 2192
HincII	GTYRAC	4 cut(s)	144, 178, 2344, 2488
HindII	GTYRAC	4 cut(s)	144, 178, 2344, 2488
HindIII	AAGCTT	2 cut(s)	480, 1228
HpaII	CCGG	8 cut(s)	234, 306, 387, 893, 1016, 1214, 1415, 2673
HphI	GGTGA	7 cut(s)	193, 265, 364, 970, 1315, 1670, 2102
Hpy166II	GTNNAC	8 cut(s)	144, 178, 352, 807, 906, 1637, 2344, 2488
Hpy188III	TCNNGA	8 cut(s)	289, 406, 446, 621, 1250, 2381, 2450, 2673
Hpy8I	GTNNAC	8 cut(s)	144, 178, 352, 807, 906, 1637, 2344, 2488
Hpy99I	CGWCG	3 cut(s)	86, 95, 1378
HpyCH4III	ACNGT	5 cut(s)	173, 910, 925, 1105, 1303
HpyCH4IV	ACGT	3 cut(s)	93, 614, 855
HpyF10VI	GCNNNNNNNGC	9 cut(s)	74, 399, 408, 1227, 1871, 2165, 2429, 2581, 2590
HpySE526I	ACGT	3 cut(s)	93, 614, 855
Hsp92I	GRCGYC	2 cut(s)	93, 309
HspAI	GCGC	5 cut(s)	309, 1608, 1910, 2002, 2192
KasI	GGCGCC	1 cut(s)	308
Kpn2I	TCCGGA	1 cut(s)	2672
KroI	GCCGGC	2 cut(s)	386, 892
KroNI	GCCGGC	2 cut(s)	388, 894
Kzo9I	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
LmnI	GCTCC	4 cut(s)	469, 680, 795, 1065
Lsp1109I	GCAGC	6 cut(s)	174, 377, 490, 864, 972, 2273
LweI	GCATC	3 cut(s)	1433, 2559, 2664
MaeI	CTAG	3 cut(s)	35, 1404, 2613
MaeII	ACGT	3 cut(s)	93, 614, 855
MalI	GATC	9 cut(s)	59, 625, 749, 756, 783, 969, 1083, 1700, 1921
MboI	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
MboII	GAAGA	6 cut(s)	327, 776, 834, 862, 2546, 2549
MflI	RGATCY	3 cut(s)	623, 754, 1919
MhlI	GDGCHC	4 cut(s)	474, 822, 1352, 2514
MlsI	TGGCCA	1 cut(s)	326
MluNI	TGGCCA	1 cut(s)	326
Mly113I	GGCGCC	1 cut(s)	309
MmeI	TCCRAC	2 cut(s)	114, 1284
Mox20I	TGGCCA	1 cut(s)	326
MroI	TCCGGA	1 cut(s)	2672
MroNI	GCCGGC	2 cut(s)	386, 892
MroXI	GAANNNNTTC	1 cut(s)	2355
MscI	TGGCCA	1 cut(s)	326
MseI	TTAA	6 cut(s)	648, 1226, 1568, 1979, 2120, 2184
MslI	CAYNNNNRTG	2 cut(s)	830, 1128
Msp20I	TGGCCA	1 cut(s)	326
MspA1I	CMGCKG	2 cut(s)	165, 973
MspI	CCGG	8 cut(s)	234, 306, 387, 893, 1016, 1214, 1415, 2673
MspR9I	CCNGG	4 cut(s)	713, 957, 1449, 2495
MvaI	CCWGG	4 cut(s)	713, 957, 1449, 2495
MvnI	CGCG	3 cut(s)	795, 2004, 2192
MwoI	GCNNNNNNNGC	9 cut(s)	74, 399, 408, 1227, 1871, 2165, 2429, 2581, 2590
NaeI	GCCGGC	2 cut(s)	388, 894
NarI	GGCGCC	1 cut(s)	309
NdeII	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
NgoMIV	GCCGGC	2 cut(s)	386, 892
NlaIV	GGNNCC	7 cut(s)	310, 676, 885, 1135, 1921, 2327, 2499
NmuCI	GTSAC	6 cut(s)	199, 493, 976, 1658, 1717, 2275
NspI	RCATGY	2 cut(s)	1966, 2483
PaeR7I	CTCGAG	1 cut(s)	406
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 404
PdiI	GCCGGC	2 cut(s)	388, 894
PdmI	GAANNNNTTC	1 cut(s)	2355
PfeI	GAWTC	5 cut(s)	548, 631, 1031, 1823, 2693
PflFI	GACNNNGTC	1 cut(s)	173
PflMI	CCANNNNNTGG	2 cut(s)	138, 1571
PkrI	GCNGC	7 cut(s)	79, 164, 392, 505, 879, 987, 2263
PluTI	GGCGCC	1 cut(s)	312
PpuMI	RGGWCCY	1 cut(s)	2498
PsiI	TTATAA	1 cut(s)	1776
Psp124BI	GAGCTC	1 cut(s)	474
Psp1406I	AACGTT	2 cut(s)	614, 855
Psp5II	RGGWCCY	1 cut(s)	2498
Psp6I	CCWGG	4 cut(s)	711, 955, 1447, 2493
PspEI	GGTNACC	2 cut(s)	976, 1658
PspFI	CCCAGC	2 cut(s)	165, 267
PspGI	CCWGG	4 cut(s)	711, 955, 1447, 2493
PspN4I	GGNNCC	7 cut(s)	310, 676, 885, 1135, 1921, 2327, 2499
PspPPI	RGGWCCY	1 cut(s)	2498
PstI	CTGCAG	3 cut(s)	164, 508, 882
PstNI	CAGNNNCTG	1 cut(s)	2267
PsuI	RGATCY	3 cut(s)	623, 754, 1919
PsyI	GACNNNGTC	1 cut(s)	173
PvuII	CAGCTG	1 cut(s)	165
RsaI	GTAC	6 cut(s)	1369, 1381, 1619, 1961, 2459, 2608
RsaNI	GTAC	6 cut(s)	1368, 1380, 1618, 1960, 2458, 2607
RseI	CAYNNNNRTG	2 cut(s)	830, 1128
SacI	GAGCTC	1 cut(s)	474
SaqAI	TTAA	6 cut(s)	648, 1226, 1568, 1979, 2120, 2184
SatI	GCNGC	7 cut(s)	78, 163, 391, 504, 878, 986, 2262
Sau3AI	GATC	9 cut(s)	57, 623, 747, 754, 781, 967, 1081, 1698, 1919
ScrFI	CCNGG	4 cut(s)	713, 957, 1449, 2495
SduI	GDGCHC	4 cut(s)	474, 822, 1352, 2514
SfaNI	GCATC	3 cut(s)	1433, 2559, 2664
SfcI	CTRYAG	3 cut(s)	160, 504, 878
SfoI	GGCGCC	1 cut(s)	310
Sfr274I	CTCGAG	1 cut(s)	406
SinI	GGWCC	8 cut(s)	599, 743, 884, 903, 1134, 1433, 1876, 2498
SlaI	CTCGAG	1 cut(s)	406
SmiMI	CAYNNNNRTG	2 cut(s)	830, 1128
SmlI	CTYRAG	2 cut(s)	406, 989
SmoI	CTYRAG	2 cut(s)	406, 989
SspDI	GGCGCC	1 cut(s)	308
SspI	AATATT	1 cut(s)	2078
SspMI	CTAG	3 cut(s)	35, 1404, 2613
SstI	GAGCTC	1 cut(s)	474
StyD4I	CCNGG	4 cut(s)	711, 955, 1447, 2493
StyI	CCWWGG	1 cut(s)	2160
TaaI	ACNGT	5 cut(s)	173, 910, 925, 1105, 1303
TaiI	ACGT	3 cut(s)	96, 617, 858
TaqI	TCGA	9 cut(s)	357, 407, 447, 546, 780, 1208, 1697, 2553, 2707
TatI	WGTACW	1 cut(s)	1959
TauI	GCSGC	1 cut(s)	80
TfiI	GAWTC	5 cut(s)	548, 631, 1031, 1823, 2693
Tru1I	TTAA	6 cut(s)	648, 1226, 1568, 1979, 2120, 2184
Tru9I	TTAA	6 cut(s)	648, 1226, 1568, 1979, 2120, 2184
TseFI	GTSAC	6 cut(s)	199, 493, 976, 1658, 1717, 2275
TseI	GCWGC	6 cut(s)	162, 390, 503, 877, 985, 2261
Tsp45I	GTSAC	6 cut(s)	199, 493, 976, 1658, 1717, 2275
TspDTI	ATGAA	6 cut(s)	1060, 1477, 1655, 2095, 2547, 2616
TspGWI	ACGGA	2 cut(s)	1362, 1831
Tth111I	GACNNNGTC	1 cut(s)	173
Van91I	CCANNNNNTGG	2 cut(s)	138, 1571
VpaK11BI	GGWCC	8 cut(s)	599, 743, 884, 903, 1134, 1433, 1876, 2498
XagI	CCTNNNNNAGG	1 cut(s)	1883
XapI	RAATTY	5 cut(s)	517, 1108, 1174, 1289, 1544
XceI	RCATGY	2 cut(s)	1966, 2483
XhoI	CTCGAG	1 cut(s)	406
XmiI	GTMKAC	1 cut(s)	351
XmnI	GAANNNNTTC	1 cut(s)	2355
XspI	CTAG	3 cut(s)	35, 1404, 2613
ZraI	GACGTC	1 cut(s)	94

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.