Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0006862

Physical Location & Seq

Genomic Coordinates Reverse (-)

1434643 .. 1436412

Gene Structure 1770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g01540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGATTGCTGGTATAGTTATTGCCGTTGTTATTTTCGTTGTGGTAGTATTGTTTGTGTCCTACAAATGTTATGCTAGTAAAAAGCATAAGAAATTTGGAAGGGTGGCTAATCCATTAACTGGGATAGAAATTGCTAAGAGTGATGTTATGAGTAGCGCAAATGGGTACAATGGAGTTACAAGTGAACTGCAAAGCCAGAGCAGTGGTGACCTTCATGTTTTTGAGGGTGGAAATGTTGCGATTTCGATCCAAGTTCTAAGACAAGTGACAAACAATTTTAGCGAGGACAACATATTAGGCAGAGGAGGATTTGGAATTGTTTATAAAGGCGAATTGCATGATGGGACCAAAATTGCCGTCAAACGGATGGAATCTGTGGCAGTAGGCACTAAGGGATTGAACGAGTTTCAGGCAGAAATCGCAGTCCTTACTAAGGTCAGGCATAGGCATTTGGTTGCGCTTTTGGGATCCTGTATCAATGGCAATGAGAGACTTTTGGTCTACGAGTACATGCCACAAGGGACTTTAACACAGCATTTATTTGAATGGCGCGAGATTGGAGTGCCTCCACTGACTTGGAAGCAGAGAGTAACAATAGCATTGGATGTGGCACGAGGAGTGGAATATTTGCACAGCTTGGCTCAACAAAGTTTCATTCACAGAGATTTAAAGCCCTCAAACATACTTCTGGGCGATGACATGAGGGCCAAGGTTGCTGACTTCGGACTGGTTAAAAACGCGCCCGACGGGAAGTATTCTGTTGAGACAAGGTTGGCAGGGACATTCGGGTATCTCGCACCAGAATATGCAGCTACTGGCAGAGTCACTACAAAAGTAGATGTTTATGCTTTCGGAGTGGTTTTGATGGAGTTGATAAGCGGTAGAAAAGCTCTCGATGATACAATGCCAGATGAGCGGTCTCATTTGGTCTCATGGTTCCGCAGAGTCCTTGTCAACAAAGAAAACATTCCAAAGGCTATTGACCAAACTCTTGACCCGGATGAGGAGACAATGGAGAGCATATACAAAGTTGCTGAGCTGGCAGGATACTGCACTGCTCGTGAGCCGTACCAGAGACCAGACATGGGACATGCTGTCAACATCCTGGGTCCTCTTGTTGAGCACTGGAAGCCTTCCACCCATGAAGAAGAAAACTCTGGCATCGACCTGCACATGAGCCTTCCGCAAGCGTTGCAAAGATGGCAAGCCAATGAAGGTACTTCTAGGATGTTTGATGATATGTCCTTCTCTCAAACTCAATCAAGCATCCCTTCAAAACCTTCCGGATTTGCTGACTCATTTGATTCAATGGATTGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase_Tyr

Protein Analysis

441

Amino Acids

48.77

Weight (kDa)

6.13

Isoelectric Point (pI)

42.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	94 - 366	7.6e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	96 - 364	6.6e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23750
fragaria_vesca	FvH4_4g13110 FvH4_4g13110
malus_domestica	MD04G1019100.v1.1
prunus_persica	Prupe.1G168400_v2.0.a1 Prupe.1G168500_v2.0.a1 Prupe.1G168500_v2.0.a1
pyrus_communis	pycom04g01540 pycom13g29180
rosa_chinensis	RchiOBHm_Chr4g0412891 RchiOBHm_Chr4g0413671
rosa_laevigata	RLG00000008202 RLG00000008250
rosa_multiflora	Rmu_sc0004133.1_g000014 Rmu_sc0005621.1_g000017 Rmu_sc0012279.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357130
rosa_rugosa	Rorug04G0117300
rosa_samantha	Rh4AG176200 Rh4AG181600 Rh4BG176600 Rh4CG187200 Rh4DG173400 Rh4DG177100
rosa_wichuraiana	Rw4G014790 Rw4G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	324
Acc36I	ACCTGC	1 cut(s)	1176
AccB7I	CCANNNNNTGG	1 cut(s)	119
AccBSI	CCGCTC	1 cut(s)	916
AccI	GTMKAC	1 cut(s)	501
AccII	CGCG	2 cut(s)	552, 740
AccIII	TCCGGA	1 cut(s)	1283
AciI	CCGC	4 cut(s)	879, 916, 940, 1184
AclWI	GGATC	3 cut(s)	241, 462, 475
AcsI	RAATTY	1 cut(s)	92
AfaI	GTAC	4 cut(s)	167, 509, 1070, 1219
AfiI	CCNNNNNNNGG	5 cut(s)	119, 363, 433, 1003, 1085
AgsI	TTSAA	4 cut(s)	400, 545, 1275, 1308
AhdI	GACNNNNNGTC	2 cut(s)	497, 1094
AjnI	CCWGG	1 cut(s)	1104
AluBI	AGCT	4 cut(s)	636, 812, 890, 1039
AluI	AGCT	4 cut(s)	636, 812, 890, 1039
Alw21I	GWGCWC	1 cut(s)	1125
Alw26I	GTCTC	6 cut(s)	484, 758, 924, 934, 1001, 1069
AlwI	GGATC	3 cut(s)	241, 462, 475
AlwNI	CAGNNNCTG	1 cut(s)	815
Aor13HI	TCCGGA	1 cut(s)	1283
AoxI	GGCC	1 cut(s)	705
ApeKI	GCWGC	1 cut(s)	809
ApoI	RAATTY	1 cut(s)	92
ArsI	GACNNNNNNTTYG	2 cut(s)	342, 374
Asp700I	GAANNNNTTC	1 cut(s)	966
AspLEI	GCGC	4 cut(s)	158, 460, 552, 742
AspS9I	GGNCC	3 cut(s)	345, 705, 1109
AsuC2I	CCSGG	1 cut(s)	998
AsuHPI	GGTGA	1 cut(s)	218
AvaII	GGWCC	2 cut(s)	345, 1109
BamHI	GGATCC	1 cut(s)	467
BauI	CACGAG	2 cut(s)	612, 1059
Bbv12I	GWGCWC	1 cut(s)	1125
BbvI	GCAGC	1 cut(s)	821
BccI	CCATC	4 cut(s)	335, 361, 859, 1194
BceAI	ACGGC	3 cut(s)	8, 341, 1051
BciT130I	CCWGG	1 cut(s)	1106
BciVI	GTATCC	1 cut(s)	1040
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	6 cut(s)	484, 758, 924, 934, 1001, 1069
BfaI	CTAG	2 cut(s)	75, 1224
BfuAI	ACCTGC	1 cut(s)	1176
BfuI	GTATCC	1 cut(s)	1040
BisI	GCNGC	1 cut(s)	810
BlpI	GCTNAGC	1 cut(s)	1035
BlsI	GCNGC	1 cut(s)	811
Bme1390I	CCNGG	2 cut(s)	998, 1106
Bme18I	GGWCC	2 cut(s)	345, 1109
BmeRI	GACNNNNNGTC	2 cut(s)	497, 1094
BmgT120I	GGNCC	3 cut(s)	345, 705, 1109
BmiI	GGNNCC	4 cut(s)	346, 469, 938, 1110
BmrFI	CCNGG	2 cut(s)	998, 1106
BmrI	ACTGGG	1 cut(s)	129
BmsI	GCATC	2 cut(s)	1170, 1275
BmuI	ACTGGG	1 cut(s)	129
Bpu1102I	GCTNAGC	1 cut(s)	1035
BpuMI	CCSGG	1 cut(s)	998
BsaBI	GATNNNNATC	1 cut(s)	245
BsaI	GGTCTC	3 cut(s)	924, 934, 1069
BsaJI	CCNNGG	2 cut(s)	708, 1105
BsaWI	WCCGGW	1 cut(s)	1283
Bsc4I	CCNNNNNNNGG	5 cut(s)	119, 363, 433, 1003, 1085
Bse1I	ACTGG	4 cut(s)	124, 732, 820, 1130
Bse3DI	GCAATG	1 cut(s)	490
Bse8I	GATNNNNATC	1 cut(s)	245
BseAI	TCCGGA	1 cut(s)	1283
BseBI	CCWGG	1 cut(s)	1106
BseDI	CCNNGG	2 cut(s)	708, 1105
BseGI	GGATG	6 cut(s)	372, 610, 1006, 1101, 1233, 1266
BseJI	GATNNNNATC	1 cut(s)	245
BseLI	CCNNNNNNNGG	5 cut(s)	119, 363, 433, 1003, 1085
BseMI	GCAATG	1 cut(s)	490
BseMII	CTCAG	1 cut(s)	1026
BseNI	ACTGG	4 cut(s)	124, 732, 820, 1130
BseRI	GAGGAG	3 cut(s)	318, 630, 1019
BseXI	GCAGC	1 cut(s)	821
BsgI	GTGCAG	2 cut(s)	1036, 1154
Bsh1236I	CGCG	2 cut(s)	552, 740
BshFI	GGCC	1 cut(s)	707
BsiHKAI	GWGCWC	1 cut(s)	1125
BsiSI	CCGG	2 cut(s)	998, 1284
BslFI	GGGAC	4 cut(s)	358, 535, 793, 1101
BslI	CCNNNNNNNGG	5 cut(s)	119, 363, 433, 1003, 1085
BsmAI	GTCTC	6 cut(s)	484, 758, 924, 934, 1001, 1069
BsmFI	GGGAC	4 cut(s)	358, 535, 793, 1101
BsnI	GGCC	1 cut(s)	707
Bso31I	GGTCTC	3 cut(s)	924, 934, 1069
Bsp1286I	GDGCHC	1 cut(s)	1125
Bsp13I	TCCGGA	1 cut(s)	1283
Bsp143I	GATC	2 cut(s)	246, 467
Bsp1720I	GCTNAGC	1 cut(s)	1035
BspACI	CCGC	4 cut(s)	879, 916, 940, 1184
BspANI	GGCC	1 cut(s)	707
BspCNI	CTCAG	1 cut(s)	1027
BspEI	TCCGGA	1 cut(s)	1283
BspFNI	CGCG	2 cut(s)	552, 740
BspLI	GGNNCC	4 cut(s)	346, 469, 938, 1110
BspMI	ACCTGC	1 cut(s)	1176
BspPI	GGATC	3 cut(s)	241, 462, 475
BspTNI	GGTCTC	3 cut(s)	924, 934, 1069
BsrBI	CCGCTC	1 cut(s)	916
BsrDI	GCAATG	1 cut(s)	490
BsrI	ACTGG	4 cut(s)	124, 732, 820, 1130
BssECI	CCNNGG	2 cut(s)	708, 1105
BssMI	GATC	2 cut(s)	246, 467
BssSI	CACGAG	2 cut(s)	612, 1059
BssT1I	CCWWGG	1 cut(s)	708
Bst2BI	CACGAG	2 cut(s)	612, 1059
Bst2UI	CCWGG	1 cut(s)	1106
BstAPI	GCANNNNNTGC	1 cut(s)	1192
BstC8I	GCNNGC	3 cut(s)	1041, 1188, 1206
BstDEI	CTNAG	5 cut(s)	135, 257, 390, 432, 1035
BstEII	GGTNACC	1 cut(s)	206
BstENI	CCTNNNNNAGG	1 cut(s)	431
BstF5I	GGATG	6 cut(s)	372, 610, 1006, 1101, 1233, 1266
BstFNI	CGCG	2 cut(s)	552, 740
BstHHI	GCGC	4 cut(s)	158, 460, 552, 742
BstKTI	GATC	2 cut(s)	249, 470
BstMAI	GTCTC	6 cut(s)	484, 758, 924, 934, 1001, 1069
BstMBI	GATC	2 cut(s)	246, 467
BstMWI	GCNNNNNNNGC	7 cut(s)	419, 713, 913, 1040, 1129, 1192, 1201
BstNI	CCWGG	1 cut(s)	1106
BstNSI	RCATGY	2 cut(s)	514, 1094
BstPI	GGTNACC	1 cut(s)	206
BstSCI	CCNGG	2 cut(s)	996, 1104
BstUI	CGCG	2 cut(s)	552, 740
BstV1I	GCAGC	1 cut(s)	821
BstX2I	RGATCY	1 cut(s)	467
BstXI	CCANNNNNNTGG	2 cut(s)	203, 576
BstYI	RGATCY	1 cut(s)	467
BsuI	GTATCC	1 cut(s)	1040
BsuRI	GGCC	1 cut(s)	707
BtgZI	GCGATG	1 cut(s)	708
BtsCI	GGATG	6 cut(s)	372, 610, 1006, 1101, 1233, 1266
BtsI	GCAGTG	2 cut(s)	208, 1053
BtsIMutI	CAGTG	4 cut(s)	208, 569, 1053, 1123
BveI	ACCTGC	1 cut(s)	1176
Cac8I	GCNNGC	3 cut(s)	1041, 1188, 1206
CaiI	CAGNNNCTG	1 cut(s)	815
CfoI	GCGC	4 cut(s)	158, 460, 552, 742
Cfr13I	GGNCC	3 cut(s)	345, 705, 1109
Csp6I	GTAC	4 cut(s)	166, 508, 1069, 1218
CviAII	CATG	9 cut(s)	215, 338, 511, 700, 933, 1084, 1091, 1142, 1174
CviQI	GTAC	4 cut(s)	166, 508, 1069, 1218
DdeI	CTNAG	5 cut(s)	135, 257, 390, 432, 1035
DpnI	GATC	2 cut(s)	248, 469
DpnII	GATC	2 cut(s)	246, 467
DraI	TTTAAA	1 cut(s)	669
DriI	GACNNNNNGTC	2 cut(s)	497, 1094
Eam1105I	GACNNNNNGTC	2 cut(s)	497, 1094
Eco130I	CCWWGG	1 cut(s)	708
Eco31I	GGTCTC	3 cut(s)	924, 934, 1069
Eco47I	GGWCC	2 cut(s)	345, 1109
Eco91I	GGTNACC	1 cut(s)	206
EcoNI	CCTNNNNNAGG	1 cut(s)	431
EcoO109I	RGGNCCY	1 cut(s)	1109
EcoO65I	GGTNACC	1 cut(s)	206
EcoRII	CCWGG	1 cut(s)	1104
EcoT14I	CCWWGG	1 cut(s)	708
ErhI	CCWWGG	1 cut(s)	708
FaeI	CATG	9 cut(s)	218, 341, 514, 703, 936, 1087, 1094, 1145, 1177
FalI	AAGNNNNNCTT	2 cut(s)	1255, 1287
FaqI	GGGAC	4 cut(s)	358, 535, 793, 1101
FatI	CATG	9 cut(s)	214, 337, 510, 699, 932, 1083, 1090, 1141, 1173
FblI	GTMKAC	1 cut(s)	501
Fnu4HI	GCNGC	1 cut(s)	810
FokI	GGATG	6 cut(s)	379, 617, 1013, 1088, 1240, 1253
Fsp4HI	GCNGC	1 cut(s)	810
FspBI	CTAG	2 cut(s)	75, 1224
GlaI	GCGC	4 cut(s)	157, 459, 551, 741
GluI	GCNGC	1 cut(s)	810
HaeIII	GGCC	1 cut(s)	707
HapII	CCGG	2 cut(s)	998, 1284
HhaI	GCGC	4 cut(s)	158, 460, 552, 742
Hin1II	CATG	9 cut(s)	218, 341, 514, 703, 936, 1087, 1094, 1145, 1177
Hin6I	GCGC	4 cut(s)	156, 458, 550, 740
HinP1I	GCGC	4 cut(s)	156, 458, 550, 740
HincII	GTYRAC	2 cut(s)	955, 1099
HindII	GTYRAC	2 cut(s)	955, 1099
HinfI	GANTC	5 cut(s)	371, 822, 945, 1295, 1304
HpaII	CCGG	2 cut(s)	998, 1284
HphI	GGTGA	1 cut(s)	218
Hpy166II	GTNNAC	4 cut(s)	185, 502, 955, 1099
Hpy188I	TCNGA	2 cut(s)	725, 854
Hpy188III	TCNNGA	4 cut(s)	893, 992, 1061, 1284
Hpy8I	GTNNAC	4 cut(s)	185, 502, 955, 1099
Hpy99I	CGWCG	1 cut(s)	749
HpyAV	CCTTC	8 cut(s)	93, 221, 1143, 1190, 1208, 1255, 1281, 1290
HpyCH4V	TGCA	7 cut(s)	190, 337, 631, 809, 1053, 1171, 1195
HpyF10VI	GCNNNNNNNGC	7 cut(s)	419, 713, 913, 1040, 1129, 1192, 1201
HpyF3I	CTNAG	5 cut(s)	135, 257, 390, 432, 1035
Hsp92II	CATG	9 cut(s)	218, 341, 514, 703, 936, 1087, 1094, 1145, 1177
HspAI	GCGC	4 cut(s)	156, 458, 550, 740
Kpn2I	TCCGGA	1 cut(s)	1283
Kzo9I	GATC	2 cut(s)	246, 467
Lsp1109I	GCAGC	1 cut(s)	821
LweI	GCATC	2 cut(s)	1170, 1275
MaeI	CTAG	2 cut(s)	75, 1224
MaeIII	GTNAC	5 cut(s)	175, 206, 265, 589, 823
MalI	GATC	2 cut(s)	248, 469
MbiI	CCGCTC	1 cut(s)	916
MboI	GATC	2 cut(s)	246, 467
MboII	GAAGA	2 cut(s)	1157, 1160
MflI	RGATCY	1 cut(s)	467
MhlI	GDGCHC	1 cut(s)	1125
MluCI	AATT	6 cut(s)	92, 129, 274, 315, 332, 351
MlyI	GAGTC	3 cut(s)	831, 954, 1289
MroI	TCCGGA	1 cut(s)	1283
MroXI	GAANNNNTTC	1 cut(s)	966
MseI	TTAA	4 cut(s)	116, 527, 668, 732
MspI	CCGG	2 cut(s)	998, 1284
MspR9I	CCNGG	2 cut(s)	998, 1106
MvaI	CCWGG	1 cut(s)	1106
MvnI	CGCG	2 cut(s)	552, 740
MwoI	GCNNNNNNNGC	7 cut(s)	419, 713, 913, 1040, 1129, 1192, 1201
NciI	CCSGG	1 cut(s)	998
NdeII	GATC	2 cut(s)	246, 467
NlaIII	CATG	9 cut(s)	218, 341, 514, 703, 936, 1087, 1094, 1145, 1177
NlaIV	GGNNCC	4 cut(s)	346, 469, 938, 1110
NmuCI	GTSAC	3 cut(s)	206, 265, 823
NspI	RCATGY	2 cut(s)	514, 1094
PdmI	GAANNNNTTC	1 cut(s)	966
PfeI	GAWTC	2 cut(s)	371, 1304
PflMI	CCANNNNNTGG	1 cut(s)	119
PkrI	GCNGC	1 cut(s)	811
PleI	GAGTC	3 cut(s)	830, 953, 1289
PpsI	GAGTC	3 cut(s)	830, 953, 1289
PpuMI	RGGWCCY	1 cut(s)	1109
PsiI	TTATAA	1 cut(s)	324
Psp5II	RGGWCCY	1 cut(s)	1109
Psp6I	CCWGG	1 cut(s)	1104
PspEI	GGTNACC	1 cut(s)	206
PspGI	CCWGG	1 cut(s)	1104
PspN4I	GGNNCC	4 cut(s)	346, 469, 938, 1110
PspPI	GGNCC	3 cut(s)	345, 705, 1109
PspPPI	RGGWCCY	1 cut(s)	1109
PstNI	CAGNNNCTG	1 cut(s)	815
PsuI	RGATCY	1 cut(s)	467
RsaI	GTAC	4 cut(s)	167, 509, 1070, 1219
RsaNI	GTAC	4 cut(s)	166, 508, 1069, 1218
SaqAI	TTAA	4 cut(s)	116, 527, 668, 732
SatI	GCNGC	1 cut(s)	810
Sau3AI	GATC	2 cut(s)	246, 467
Sau96I	GGNCC	3 cut(s)	345, 705, 1109
SchI	GAGTC	3 cut(s)	831, 954, 1289
ScrFI	CCNGG	2 cut(s)	998, 1106
SduI	GDGCHC	1 cut(s)	1125
SfaNI	GCATC	2 cut(s)	1170, 1275
SinI	GGWCC	2 cut(s)	345, 1109
Sse9I	AATT	6 cut(s)	92, 129, 274, 315, 332, 351
SsiI	CCGC	4 cut(s)	879, 916, 940, 1184
SspI	AATATT	1 cut(s)	626
SspMI	CTAG	2 cut(s)	75, 1224
StyD4I	CCNGG	2 cut(s)	996, 1104
StyI	CCWWGG	1 cut(s)	708
TaqI	TCGA	4 cut(s)	245, 894, 1164, 1318
TasI	AATT	6 cut(s)	92, 129, 274, 315, 332, 351
TatI	WGTACW	1 cut(s)	507
TfiI	GAWTC	2 cut(s)	371, 1304
Tru1I	TTAA	4 cut(s)	116, 527, 668, 732
Tru9I	TTAA	4 cut(s)	116, 527, 668, 732
TscAI	CASTG	4 cut(s)	208, 576, 1060, 1130
TseFI	GTSAC	3 cut(s)	206, 265, 823
TseI	GCWGC	1 cut(s)	809
Tsp45I	GTSAC	3 cut(s)	206, 265, 823
TspDTI	ATGAA	4 cut(s)	203, 643, 1158, 1227
TspGWI	ACGGA	1 cut(s)	379
TspRI	CASTG	4 cut(s)	208, 576, 1060, 1130
Van91I	CCANNNNNTGG	1 cut(s)	119
VpaK11BI	GGWCC	2 cut(s)	345, 1109
XagI	CCTNNNNNAGG	1 cut(s)	431
XapI	RAATTY	1 cut(s)	92
XceI	RCATGY	2 cut(s)	514, 1094
XmiI	GTMKAC	1 cut(s)	501
XmnI	GAANNNNTTC	1 cut(s)	966
XspI	CTAG	2 cut(s)	75, 1224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.