AT3G23870

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003718
Physical Location & Seq
Forward (+)
8619192 .. 8621949
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23870.5

Sequence Viewer

Length: 1008 bp
ATGGATCAAATGTCACCTGATAACATAAATGGAGTCATTTTAGCAGTGTCTTCAAGTATCTTTATTGGTTCTAGCTTCATCATCAAGAAAAAAGGTCTCAAGAAAGCCGGTGCAAGCGGTGTCCGAGCAGGTGAAGGAGGGTATGGATACTTGAAGGAGCCATGGTGGTGGGCTGGAATGATAACAATGATTGTTGGGGAAGTAGCTAATTTTGCAGCCTATGCATTTGCACCGGCTATTTTGGTAACACCTTTGGGAGCTTTAAGTATTATATTCAGCGCAGTGCTCGCGCATTTTATTTTGAAAGAGAAATTGCATATGTTTGGGATACTTGGTTGCATTCTCTGTGTTGTTGGGTCTACAACTATAGTCTTACACGCACCACATGAACAGAAAATAGAATCAGTCAAGCAAATATGGCAGCTTGCTATTGAACCAGGTTTCCTTGTGTATTCTGCTGTGATTGTGATTGTGGTGGCTATACTGATCTTCTACTATGAACCGCGCTACGGGAAGACTCATATGATCGTATATGTCGGAATCTGCTCCTTAATGGGTTCTCTTACTGTTATGAGTGTCAAAGCTGTGGCGATTGCGATTAAACTGACATTTTCAGGGACGAATCAGTTTAAATACTTCAACACTTGGATTTTCATTTTAGTAGTTGCAACTTGTTGCATTTTGCAGATCAACTACTTGAACAAGGCATTGGATACATTCAACACAGCGGTTATTTCTCCGGTTTACTACGTTATGTTCACAACATTCACAATCATAGCTAGTATGATCATGTTCAAGGATTGGGCTTCTCAGAGTGGATTAAAGATTGCAACAGAGTTGTGTGGTTTTGTGACGATTTTATCAGGAACTTTTCTGCTTCACAAGACCAAAGATATGGGAAACAGTGCAAGTGGTCGTGGTTCTATCTCAATGCCTACTAGAGACACTCCTGTCTTCACTAATTCAGGATCCGGTCGGAGCTCCAGCTCTGATAAAGTAGCTTCCTGA

Protein Analysis

335

Amino Acids

36.29

Weight (kDa)

9.2

Isoelectric Point (pI)

27.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 9 - 219 4.9e-98 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
AasI GACNNNNNNGTC 1 cut(s) 950
Acc36I ACCTGC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 359
AccII CGCG 2 cut(s) 290, 505
AciI CCGC 3 cut(s) 117, 503, 728
AclWI GGATC 3 cut(s) 12, 963, 976
AfiI CCNNNNNNNGG 1 cut(s) 509
AgsI TTSAA 8 cut(s) 54, 154, 304, 434, 640, 700, 721, 796
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 9 cut(s) 75, 206, 260, 424, 584, 779, 981, 987, 1001
AluI AGCT 9 cut(s) 75, 206, 260, 424, 584, 779, 981, 987, 1001
Alw21I GWGCWC 2 cut(s) 288, 983
Alw26I GTCTC 2 cut(s) 101, 936
AlwI GGATC 3 cut(s) 12, 963, 976
ApeKI GCWGC 2 cut(s) 215, 421
AspLEI GCGC 3 cut(s) 281, 292, 507
AsuHPI GGTGA 2 cut(s) 6, 143
BamHI GGATCC 1 cut(s) 968
BanII GRGCYC 1 cut(s) 983
BbsI GAAGAC 3 cut(s) 42, 521, 946
Bbv12I GWGCWC 2 cut(s) 288, 983
BbvI GCAGC 2 cut(s) 227, 433
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 3 cut(s) 140, 321, 706
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 2 cut(s) 101, 936
BfaI CTAG 3 cut(s) 72, 780, 939
BfmI CTRYAG 1 cut(s) 366
BfuAI ACCTGC 1 cut(s) 119
BfuI GTATCC 3 cut(s) 140, 321, 706
BisI GCNGC 2 cut(s) 216, 422
BlsI GCNGC 2 cut(s) 217, 423
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 159, 970
BmrFI CCNGG 1 cut(s) 438
BpiI GAAGAC 3 cut(s) 42, 521, 946
BpmI CTGGAG 1 cut(s) 967
BpuEI CTTGAG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 2 cut(s) 739, 971
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse118I RCCGGY 2 cut(s) 107, 232
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 161
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 1 cut(s) 824
BseXI GCAGC 2 cut(s) 227, 433
Bsh1236I CGCG 2 cut(s) 290, 505
Bsh1285I CGRYCG 1 cut(s) 976
BsiEI CGRYCG 1 cut(s) 976
BsiHKAI GWGCWC 2 cut(s) 288, 983
BsiSI CCGG 4 cut(s) 108, 233, 740, 972
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 2 cut(s) 101, 936
BsmFI GGGAC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 288, 983
Bsp143I GATC 6 cut(s) 4, 486, 525, 687, 786, 968
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 3 cut(s) 117, 503, 728
BspCNI CTCAG 1 cut(s) 823
BspFNI CGCG 2 cut(s) 290, 505
BspLI GGNNCC 2 cut(s) 159, 970
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 3 cut(s) 12, 963, 976
BspTNI GGTCTC 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 107, 232
BssAI RCCGGY 2 cut(s) 107, 232
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 6 cut(s) 4, 486, 525, 687, 786, 968
BssT1I CCWWGG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 568, 905
BstAPI GCANNNNNTGC 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 115, 288, 426
BstDEI CTNAG 1 cut(s) 810
BstDSI CCRYGG 1 cut(s) 161
BstFNI CGCG 2 cut(s) 290, 505
BstHHI GCGC 3 cut(s) 281, 292, 507
BstKTI GATC 6 cut(s) 7, 489, 528, 690, 789, 971
BstMAI GTCTC 2 cut(s) 101, 936
BstMBI GATC 6 cut(s) 4, 486, 525, 687, 786, 968
BstMCI CGRYCG 1 cut(s) 976
BstMWI GCNNNNNNNGC 4 cut(s) 212, 221, 287, 418
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 2 cut(s) 290, 505
BstV1I GCAGC 2 cut(s) 227, 433
BstV2I GAAGAC 3 cut(s) 42, 521, 946
BstX2I RGATCY 1 cut(s) 968
BstXI CCANNNNNNTGG 2 cut(s) 168, 895
BstYI RGATCY 1 cut(s) 968
BsuI GTATCC 3 cut(s) 140, 321, 706
BtgI CCRYGG 1 cut(s) 161
BtsI GCAGTG 2 cut(s) 51, 288
BtsIMutI CAGTG 3 cut(s) 51, 288, 910
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 115, 288, 426
CfoI GCGC 3 cut(s) 281, 292, 507
Cfr10I RCCGGY 2 cut(s) 107, 232
CsiI ACCWGGT 1 cut(s) 436
CviAII CATG 3 cut(s) 162, 386, 790
DdeI CTNAG 1 cut(s) 810
DpnI GATC 6 cut(s) 6, 488, 527, 689, 788, 970
DpnII GATC 6 cut(s) 4, 486, 525, 687, 786, 968
DraI TTTAAA 1 cut(s) 631
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
Ecl136II GAGCTC 1 cut(s) 981
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 983
Eco31I GGTCTC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 981
EcoICRI GAGCTC 1 cut(s) 981
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 983
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 3 cut(s) 165, 389, 793
FaqI GGGAC 1 cut(s) 631
FatI CATG 3 cut(s) 161, 385, 789
FauNDI CATATG 2 cut(s) 318, 522
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 2 cut(s) 216, 422
FriOI GRGCYC 1 cut(s) 983
Fsp4HI GCNGC 2 cut(s) 216, 422
FspBI CTAG 3 cut(s) 72, 780, 939
GlaI GCGC 3 cut(s) 280, 291, 506
GluI GCNGC 2 cut(s) 216, 422
GsuI CTGGAG 1 cut(s) 967
HapII CCGG 4 cut(s) 108, 233, 740, 972
HhaI GCGC 3 cut(s) 281, 292, 507
Hin1II CATG 3 cut(s) 165, 389, 793
Hin6I GCGC 3 cut(s) 279, 290, 505
HinP1I GCGC 3 cut(s) 279, 290, 505
HinfI GANTC 5 cut(s) 33, 401, 517, 540, 622
HpaII CCGG 4 cut(s) 108, 233, 740, 972
HphI GGTGA 2 cut(s) 6, 143
Hpy166II GTNNAC 3 cut(s) 360, 745, 759
Hpy188I TCNGA 5 cut(s) 125, 539, 813, 978, 991
Hpy188III TCNNGA 5 cut(s) 85, 100, 864, 966, 1005
Hpy8I GTNNAC 3 cut(s) 360, 745, 759
HpyAV CCTTC 2 cut(s) 128, 148
HpyCH4III ACNGT 2 cut(s) 568, 905
HpyCH4IV ACGT 1 cut(s) 750
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 221, 287, 418
HpyF3I CTNAG 1 cut(s) 810
HpySE526I ACGT 1 cut(s) 750
Hsp92II CATG 3 cut(s) 165, 389, 793
HspAI GCGC 3 cut(s) 279, 290, 505
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 6 cut(s) 4, 486, 525, 687, 786, 968
LmnI GCTCC 5 cut(s) 157, 257, 551, 978, 986
Lsp1109I GCAGC 2 cut(s) 227, 433
MabI ACCWGGT 1 cut(s) 436
MaeI CTAG 3 cut(s) 72, 780, 939
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 3 cut(s) 12, 244, 850
MalI GATC 6 cut(s) 6, 488, 527, 689, 788, 970
MboI GATC 6 cut(s) 4, 486, 525, 687, 786, 968
MboII GAAGA 4 cut(s) 42, 481, 526, 946
MflI RGATCY 1 cut(s) 968
MhlI GDGCHC 2 cut(s) 288, 983
MluCI AATT 3 cut(s) 208, 311, 961
MlyI GAGTC 2 cut(s) 42, 511
MmeI TCCRAC 2 cut(s) 517, 956
MnlI CCTC 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 5 cut(s) 263, 551, 600, 630, 821
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 4 cut(s) 108, 233, 740, 972
MspR9I CCNGG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 2 cut(s) 290, 505
MwoI GCNNNNNNNGC 4 cut(s) 212, 221, 287, 418
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 2 cut(s) 318, 522
NdeII GATC 6 cut(s) 4, 486, 525, 687, 786, 968
NlaIII CATG 3 cut(s) 165, 389, 793
NlaIV GGNNCC 2 cut(s) 159, 970
NmuCI GTSAC 2 cut(s) 12, 850
NsiI ATGCAT 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 119
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 401, 540, 622
PkrI GCNGC 2 cut(s) 217, 423
PleI GAGTC 2 cut(s) 41, 511
PpsI GAGTC 2 cut(s) 41, 511
Psp124BI GAGCTC 1 cut(s) 983
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 159, 970
PsuI RGATCY 1 cut(s) 968
RseI CAYNNNNRTG 1 cut(s) 166
SacI GAGCTC 1 cut(s) 983
SaqAI TTAA 5 cut(s) 263, 551, 600, 630, 821
SatI GCNGC 2 cut(s) 216, 422
Sau3AI GATC 6 cut(s) 4, 486, 525, 687, 786, 968
SchI GAGTC 2 cut(s) 42, 511
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 2 cut(s) 288, 983
SexAI ACCWGGT 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 3 cut(s) 208, 311, 961
SsiI CCGC 3 cut(s) 117, 503, 728
SspMI CTAG 3 cut(s) 72, 780, 939
SstI GAGCTC 1 cut(s) 983
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 568, 905
TaiI ACGT 1 cut(s) 753
TasI AATT 3 cut(s) 208, 311, 961
TfiI GAWTC 3 cut(s) 401, 540, 622
Tru1I TTAA 5 cut(s) 263, 551, 600, 630, 821
Tru9I TTAA 5 cut(s) 263, 551, 600, 630, 821
TscAI CASTG 3 cut(s) 51, 288, 910
TseFI GTSAC 2 cut(s) 12, 850
TseI GCWGC 2 cut(s) 215, 421
Tsp45I GTSAC 2 cut(s) 12, 850
TspDTI ATGAA 4 cut(s) 67, 402, 513, 643
TspRI CASTG 3 cut(s) 51, 288, 910
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 3 cut(s) 72, 780, 939
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.