Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003718

Physical Location & Seq

Genomic Coordinates Reverse (-)

1759526 .. 1765282

Gene Structure 5757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1016400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGGGGATATCGCGTGACAATGTTCATGGACTCACTCTTGCGGTTTCTTCAAGCATTTTCATTGGCACTAGTTTTATCATCAAGAAGAAAGGTCTTAAAAAGGCCGGCGCTACAGGAACCAGAGCAGGATTAGGAGGGTATACGTACTTGTATGAACCCTTGTGGTGGGCTGGGATGATAGCAATGATTGTTGGGGAGATAGCTAACTTTGCGGCTTATGCATTTGCTCCGGCAATTCTCGTAACTCCCTTGGGAGCTTTAAGTATTATTGTCAGTGCAGTGCTTGCTCATTTTGTTTTGAAGGAAAAATTGCATATTTTTGGTGTGCTTGGTTGTGTTCTTTGCGTGGTGGGTTCTGTAAGTATTGTATTGCATGCTCCACAAGAGAAACCAATATATTCCGTTAAGCAAGTATGGCATCATGCTACGCAGCCAGGTTTCATCATCTACACATGTATAGTATTGATTCTGGTTGCTGTCCTCATTGTCCGATATGTGCCACGCTACGGGCAAACCCACATGATCGTCTATGTTGGTATCTGTTCACTCATGGGATCTCTTACGGTTATGAGTGTGAAGGCAGTGGGAATAGCTCTGAAGCTGACGTTTTCCGGAACAAATCAATTTAAATTCTTCGAGACTTGGATTTTTGTGTTGATAGTGACAGTATGCTGTCTTTTTCAGATCAACTACTTAAACAAGGCTCTTGACACGTTTAACACTGCCGTCATATCTCCAGTCTACTACGTTATGTTCACAATATTCACCATTCTTGCCAGTGTGATCTTGTTCAAGGACTGGGAGTCACAAAATGGATCACACATAGTGACTGAACTGTGTGGTTTCATCACTATATTATCCGGTACATTTCTCCTTCACAAAACAAAGGACATGGGAAATGATCCACCCAAGGAACCCTCCGGATTCAAAAGTCCGAGCCACTTAGATTCATCCTCGAATTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0012505 GO:0015693 GO:0016020 GO:0030001 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234 GO:0070838 GO:0071944 GO:0072511 GO:0097708

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mg_trans_NIPA

Protein Analysis

322

Amino Acids

34.97

Weight (kDa)

9.08

Isoelectric Point (pI)

21.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_trans_NIPA	PF05653	6 - 298	4.3e-138	Magnesium transporter NIPA

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003718)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23870 AT3G23870 AT3G23870 AT3G23870 AT3G23870 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800 AT4G13800
fragaria_vesca	FvH4_4g13390 FvH4_4g13390
malus_domestica	MD04G1016400.v1.1 MD13G1282200.v1.1
prunus_persica	Prupe.1G163100_v2.0.a1 Prupe.1G163100_v2.0.a1 Prupe.1G163100_v2.0.a1
pyrus_communis	pycom04g01240
rosa_chinensis	RchiOBHm_Chr4g0413761
rosa_laevigata	RLG00000008195
rosa_multiflora	Rmu_sc0020809.1_g000002
rosa_roxburghii	Rroxscaffold_5G00357680
rosa_rugosa	Rorug04G0123200
rosa_samantha	Rh4AG182600 Rh4BG181800 Rh4CG194000 Rh4DG178200
rosa_wichuraiana	Rw4G015300 Rw4G015410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	140, 741
AccII	CGCG	1 cut(s)	13
AccIII	TCCGGA	2 cut(s)	611, 920
AciI	CCGC	2 cut(s)	41, 212
AclWI	GGATC	3 cut(s)	562, 823, 896
AcsI	RAATTY	2 cut(s)	629, 958
AcuI	CTGAAG	1 cut(s)	617
AdeI	CACNNNGTG	1 cut(s)	826
AfaI	GTAC	2 cut(s)	146, 865
AfiI	CCNNNNNNNGG	2 cut(s)	165, 506
AflIII	ACRYGT	2 cut(s)	452, 711
AgsI	TTSAA	4 cut(s)	51, 301, 793, 928
AhdI	GACNNNNNGTC	1 cut(s)	802
AhlI	ACTAGT	1 cut(s)	68
AjnI	CCWGG	1 cut(s)	433
AluBI	AGCT	4 cut(s)	203, 257, 593, 601
AluI	AGCT	4 cut(s)	203, 257, 593, 601
Alw26I	GTCTC	1 cut(s)	632
AlwI	GGATC	3 cut(s)	562, 823, 896
Aor13HI	TCCGGA	2 cut(s)	611, 920
AoxI	GGCC	1 cut(s)	102
ApeKI	GCWGC	1 cut(s)	430
ApoI	RAATTY	2 cut(s)	629, 958
AspLEI	GCGC	1 cut(s)	110
AsuHPI	GGTGA	1 cut(s)	757
BbvI	GCAGC	1 cut(s)	442
BceAI	ACGGC	1 cut(s)	710
BciT130I	CCWGG	1 cut(s)	435
BcoDI	GTCTC	1 cut(s)	632
BcuI	ACTAGT	1 cut(s)	68
BfaI	CTAG	1 cut(s)	69
BfmI	CTRYAG	1 cut(s)	111
BfoI	RGCGCY	1 cut(s)	111
BisI	GCNGC	2 cut(s)	213, 431
BlsI	GCNGC	2 cut(s)	214, 432
Bme1390I	CCNGG	1 cut(s)	435
BmeRI	GACNNNNNGTC	1 cut(s)	802
BmiI	GGNNCC	2 cut(s)	118, 915
BmrFI	CCNGG	1 cut(s)	435
BmrI	ACTGGG	1 cut(s)	808
BmsI	GCATC	1 cut(s)	427
BmuI	ACTGGG	1 cut(s)	808
BpmI	CTGGAG	1 cut(s)	720
BsaAI	YACGTR	1 cut(s)	144
BsaJI	CCNNGG	2 cut(s)	249, 909
BsaWI	WCCGGW	3 cut(s)	611, 860, 920
Bsc4I	CCNNNNNNNGG	2 cut(s)	165, 506
Bse118I	RCCGGY	1 cut(s)	104
Bse1I	ACTGG	3 cut(s)	737, 777, 803
Bse3DI	GCAATG	1 cut(s)	189
BseAI	TCCGGA	2 cut(s)	611, 920
BseBI	CCWGG	1 cut(s)	435
BseDI	CCNNGG	2 cut(s)	249, 909
BseGI	GGATG	2 cut(s)	180, 950
BseLI	CCNNNNNNNGG	2 cut(s)	165, 506
BseMI	GCAATG	1 cut(s)	189
BseNI	ACTGG	3 cut(s)	737, 777, 803
BseXI	GCAGC	1 cut(s)	442
BseYI	CCCAGC	1 cut(s)	170
BsgI	GTGCAG	1 cut(s)	297
Bsh1236I	CGCG	1 cut(s)	13
BshFI	GGCC	1 cut(s)	104
BsiSI	CCGG	5 cut(s)	105, 230, 612, 861, 921
BslI	CCNNNNNNNGG	2 cut(s)	165, 506
BsmAI	GTCTC	1 cut(s)	632
BsnI	GGCC	1 cut(s)	104
Bsp13I	TCCGGA	2 cut(s)	611, 920
Bsp143I	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
BspACI	CCGC	2 cut(s)	41, 212
BspANI	GGCC	1 cut(s)	104
BspEI	TCCGGA	2 cut(s)	611, 920
BspFNI	CGCG	1 cut(s)	13
BspLI	GGNNCC	2 cut(s)	118, 915
BspPI	GGATC	3 cut(s)	562, 823, 896
BsrDI	GCAATG	1 cut(s)	189
BsrFI	RCCGGY	1 cut(s)	104
BsrI	ACTGG	3 cut(s)	737, 777, 803
BssAI	RCCGGY	1 cut(s)	104
BssECI	CCNNGG	2 cut(s)	249, 909
BssMI	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
BssNAI	GTATAC	1 cut(s)	141
BssT1I	CCWWGG	2 cut(s)	249, 909
Bst1107I	GTATAC	1 cut(s)	141
Bst2UI	CCWGG	1 cut(s)	435
Bst4CI	ACNGT	3 cut(s)	565, 667, 837
BstAPI	GCANNNNNTGC	1 cut(s)	284
BstBAI	YACGTR	1 cut(s)	144
BstC8I	GCNNGC	3 cut(s)	106, 285, 375
BstDEI	CTNAG	1 cut(s)	943
BstF5I	GGATG	2 cut(s)	180, 950
BstFNI	CGCG	1 cut(s)	13
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	1 cut(s)	110
BstKTI	GATC	6 cut(s)	525, 557, 687, 786, 818, 904
BstMAI	GTCTC	1 cut(s)	632
BstMBI	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
BstMWI	GCNNNNNNNGC	4 cut(s)	209, 218, 284, 415
BstNI	CCWGG	1 cut(s)	435
BstNSI	RCATGY	2 cut(s)	377, 456
BstSCI	CCNGG	1 cut(s)	433
BstSFI	CTRYAG	1 cut(s)	111
BstSNI	TACGTA	1 cut(s)	144
BstUI	CGCG	1 cut(s)	13
BstV1I	GCAGC	1 cut(s)	442
BstX2I	RGATCY	1 cut(s)	554
BstYI	RGATCY	1 cut(s)	554
BstZ17I	GTATAC	1 cut(s)	141
BsuRI	GGCC	1 cut(s)	104
BtsCI	GGATG	2 cut(s)	180, 950
BtsI	GCAGTG	3 cut(s)	285, 588, 720
BtsIMutI	CAGTG	5 cut(s)	280, 285, 588, 720, 784
Cac8I	GCNNGC	3 cut(s)	106, 285, 375
CfoI	GCGC	1 cut(s)	110
Cfr10I	RCCGGY	1 cut(s)	104
Csp6I	GTAC	2 cut(s)	145, 864
CviAII	CATG	7 cut(s)	26, 374, 422, 453, 520, 550, 892
CviQI	GTAC	2 cut(s)	145, 864
DdeI	CTNAG	1 cut(s)	943
DpnI	GATC	6 cut(s)	524, 556, 686, 785, 817, 903
DpnII	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
DraI	TTTAAA	1 cut(s)	628
DraIII	CACNNNGTG	1 cut(s)	826
DriI	GACNNNNNGTC	1 cut(s)	802
Eam1105I	GACNNNNNGTC	1 cut(s)	802
Eco105I	TACGTA	1 cut(s)	144
Eco130I	CCWWGG	2 cut(s)	249, 909
Eco32I	GATATC	1 cut(s)	9
Eco57I	CTGAAG	1 cut(s)	617
EcoRI	GAATTC	1 cut(s)	958
EcoRII	CCWGG	1 cut(s)	433
EcoRV	GATATC	1 cut(s)	9
EcoT14I	CCWWGG	2 cut(s)	249, 909
EcoT22I	ATGCAT	1 cut(s)	223
ErhI	CCWWGG	2 cut(s)	249, 909
FaeI	CATG	7 cut(s)	29, 377, 425, 456, 523, 553, 895
FatI	CATG	7 cut(s)	25, 373, 421, 452, 519, 549, 891
FblI	GTMKAC	2 cut(s)	140, 741
Fnu4HI	GCNGC	2 cut(s)	213, 431
FokI	GGATG	2 cut(s)	187, 937
Fsp4HI	GCNGC	2 cut(s)	213, 431
FspBI	CTAG	1 cut(s)	69
GlaI	GCGC	1 cut(s)	109
GluI	GCNGC	2 cut(s)	213, 431
GsaI	CCCAGC	1 cut(s)	174
GsuI	CTGGAG	1 cut(s)	720
HaeII	RGCGCY	1 cut(s)	111
HaeIII	GGCC	1 cut(s)	104
HapII	CCGG	5 cut(s)	105, 230, 612, 861, 921
HhaI	GCGC	1 cut(s)	110
Hin1II	CATG	7 cut(s)	29, 377, 425, 456, 523, 553, 895
Hin6I	GCGC	1 cut(s)	108
HinP1I	GCGC	1 cut(s)	108
HinfI	GANTC	5 cut(s)	30, 466, 803, 924, 947
HpaII	CCGG	5 cut(s)	105, 230, 612, 861, 921
HphI	GGTGA	1 cut(s)	757
Hpy166II	GTNNAC	4 cut(s)	141, 545, 742, 756
Hpy188I	TCNGA	4 cut(s)	491, 597, 684, 936
Hpy188III	TCNNGA	6 cut(s)	82, 612, 637, 707, 921, 963
Hpy8I	GTNNAC	4 cut(s)	141, 545, 742, 756
HpyAV	CCTTC	3 cut(s)	295, 571, 884
HpyCH4III	ACNGT	3 cut(s)	565, 667, 837
HpyCH4IV	ACGT	4 cut(s)	143, 605, 713, 747
HpyCH4V	TGCA	4 cut(s)	221, 278, 313, 373
HpyF10VI	GCNNNNNNNGC	4 cut(s)	209, 218, 284, 415
HpyF3I	CTNAG	1 cut(s)	943
HpySE526I	ACGT	4 cut(s)	143, 605, 713, 747
Hsp92II	CATG	7 cut(s)	29, 377, 425, 456, 523, 553, 895
HspAI	GCGC	1 cut(s)	108
Kpn2I	TCCGGA	2 cut(s)	611, 920
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Kzo9I	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
LmnI	GCTCC	3 cut(s)	232, 254, 382
Lsp1109I	GCAGC	1 cut(s)	442
LweI	GCATC	1 cut(s)	427
MaeI	CTAG	1 cut(s)	69
MaeII	ACGT	4 cut(s)	143, 605, 713, 747
MaeIII	GTNAC	5 cut(s)	14, 241, 661, 804, 826
MalI	GATC	6 cut(s)	524, 556, 686, 785, 817, 903
MboI	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
MboII	GAAGA	3 cut(s)	39, 97, 625
MflI	RGATCY	1 cut(s)	554
MluCI	AATT	5 cut(s)	234, 308, 623, 629, 958
MlyI	GAGTC	2 cut(s)	24, 812
MnlI	CCTC	4 cut(s)	128, 491, 928, 964
Mph1103I	ATGCAT	1 cut(s)	223
MroI	TCCGGA	2 cut(s)	611, 920
MroNI	GCCGGC	1 cut(s)	104
MseI	TTAA	6 cut(s)	96, 260, 405, 627, 695, 717
MspI	CCGG	5 cut(s)	105, 230, 612, 861, 921
MspR9I	CCNGG	1 cut(s)	435
MvaI	CCWGG	1 cut(s)	435
MvnI	CGCG	1 cut(s)	13
MwoI	GCNNNNNNNGC	4 cut(s)	209, 218, 284, 415
NaeI	GCCGGC	1 cut(s)	106
NdeII	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
NgoMIV	GCCGGC	1 cut(s)	104
NlaIII	CATG	7 cut(s)	29, 377, 425, 456, 523, 553, 895
NlaIV	GGNNCC	2 cut(s)	118, 915
NmuCI	GTSAC	4 cut(s)	14, 661, 804, 826
NsiI	ATGCAT	1 cut(s)	223
NspI	RCATGY	2 cut(s)	377, 456
PaeI	GCATGC	1 cut(s)	377
PciI	ACATGT	1 cut(s)	452
PdiI	GCCGGC	1 cut(s)	106
PfeI	GAWTC	3 cut(s)	466, 924, 947
PkrI	GCNGC	2 cut(s)	214, 432
PleI	GAGTC	2 cut(s)	24, 811
PpsI	GAGTC	2 cut(s)	24, 811
Ppu21I	YACGTR	1 cut(s)	144
PscI	ACATGT	1 cut(s)	452
Psp6I	CCWGG	1 cut(s)	433
PspFI	CCCAGC	1 cut(s)	170
PspGI	CCWGG	1 cut(s)	433
PspN4I	GGNNCC	2 cut(s)	118, 915
PsuI	RGATCY	1 cut(s)	554
RsaI	GTAC	2 cut(s)	146, 865
RsaNI	GTAC	2 cut(s)	145, 864
SaqAI	TTAA	6 cut(s)	96, 260, 405, 627, 695, 717
SatI	GCNGC	2 cut(s)	213, 431
Sau3AI	GATC	6 cut(s)	522, 554, 684, 783, 815, 901
SchI	GAGTC	2 cut(s)	24, 812
ScrFI	CCNGG	1 cut(s)	435
SfaNI	GCATC	1 cut(s)	427
SfcI	CTRYAG	1 cut(s)	111
SmiI	ATTTAAAT	1 cut(s)	628
SnaBI	TACGTA	1 cut(s)	144
SpeI	ACTAGT	1 cut(s)	68
SphI	GCATGC	1 cut(s)	377
Sse9I	AATT	5 cut(s)	234, 308, 623, 629, 958
SsiI	CCGC	2 cut(s)	41, 212
SspI	AATATT	1 cut(s)	762
SspMI	CTAG	1 cut(s)	69
StyD4I	CCNGG	1 cut(s)	433
StyI	CCWWGG	2 cut(s)	249, 909
SwaI	ATTTAAAT	1 cut(s)	628
TaaI	ACNGT	3 cut(s)	565, 667, 837
TaiI	ACGT	4 cut(s)	146, 608, 716, 750
TaqI	TCGA	2 cut(s)	636, 956
TasI	AATT	5 cut(s)	234, 308, 623, 629, 958
TauI	GCSGC	1 cut(s)	215
TfiI	GAWTC	3 cut(s)	466, 924, 947
Tru1I	TTAA	6 cut(s)	96, 260, 405, 627, 695, 717
Tru9I	TTAA	6 cut(s)	96, 260, 405, 627, 695, 717
TscAI	CASTG	5 cut(s)	280, 285, 588, 727, 784
TseFI	GTSAC	4 cut(s)	14, 661, 804, 826
TseI	GCWGC	1 cut(s)	430
Tsp45I	GTSAC	4 cut(s)	14, 661, 804, 826
TspDTI	ATGAA	6 cut(s)	14, 49, 168, 430, 835, 939
TspGWI	ACGGA	1 cut(s)	391
TspRI	CASTG	5 cut(s)	280, 285, 588, 727, 784
XapI	RAATTY	2 cut(s)	629, 958
XceI	RCATGY	2 cut(s)	377, 456
XmiI	GTMKAC	2 cut(s)	140, 741
XspI	CTAG	1 cut(s)	69
Zsp2I	ATGCAT	1 cut(s)	223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.