Rw4G015410

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003718
Physical Location & Seq
Forward (+)
35393437 .. 35398033
4597 bp
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UTR
Exon/CDS
Intron
Rw4G015410.1

Sequence Viewer

Length: 978 bp
ATGGGATTGTCGCCTGACAATGTTCATGGACTCACTCTAGCGGTTTCTTCAAGCGTTTTTATTGGGACTAGCTTTATAATCAAGAAGAAAGGCCTCAAAAAAGCAGGCACTACAGGAGTTAGAGCAGGGTCTGGAGGGCATACATACTTGTATGAACCCTTATGGTGGGCTGGGATGATATCAATGATTGTGGGGGAGATAGCTAATTTTGCTGCTTATGCATTTGCTCCAGCAATTCTTGTAACTCCCTTGGGAGCATTAAGTATTATCGTCAGTGCTTGCTCATTTTGTTTTGGAGGAAAAACTGCATATATTTGGGGTGCTTGGTTGTGTTCTTTGCATGGTGGGCTCTACAACATTGTTTTGAATGCTCCACAAGAGAGACAAATAAATTCGTTTAAGCAAGTGTGGCATCATGCTACACAGCCAGGTTTTATTGTCTACACAGTTTTCGTACTGATTCTCGTTGCCGTCCTCATTTTCCAATTTGTTCCACGTTATGGGAAGACCCACCTGATCGTATATGTTGGAATTTGTTCTCTTATGGGCTCTCTCACGGTTATGAGTGTGAAAGCAGTGGGTATCGCTCTGAAGCTGTCCTTTTCAGGAACAAACCAATTTAAATACTATGAGACATGGGTTTTTGCAGTGATAGTGGCAGTCTGTTGTATTTTTCAGATTAACTATTTAAACAAGGCTCTGGACACCTTTAACACTGCTGTAATATCTCCAGTCTACTATGTGATGTTTACAATATTCACCATTCTTGCCAGCGTTATCATGTTTAAGGACTGGGAGTCGCAAAGTGCGTCTCAGATTGTGACTGAATTGTGTGGTTTTGTTACTATCCTATCTGGTACCTTTCTACTTCACAAAACCAGAGATATGGGAAATGATCCACCTAAGGAACCTCCTGTCTTCCAAAGTCCTCGTGCGTCAGAAGCACCTTCATCTCCACTTCCTATTAGCCAGAGATAG

Protein Analysis

325

Amino Acids

35.47

Weight (kDa)

9.1

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 6 - 296 1.7e-120 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
Acc65I GGTACC 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 857
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 2 cut(s) 441, 735
AciI CCGC 1 cut(s) 41
AclWI GGATC 1 cut(s) 890
AcsI RAATTY 2 cut(s) 391, 531
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 2 cut(s) 456, 859
AfiI CCNNNNNNNGG 2 cut(s) 165, 500
AgsI TTSAA 2 cut(s) 51, 367
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 3 cut(s) 72, 203, 595
AluI AGCT 3 cut(s) 72, 203, 595
Alw26I GTCTC 3 cut(s) 376, 626, 816
AlwI GGATC 1 cut(s) 890
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 2 cut(s) 391, 531
Asp700I GAANNNNTTC 1 cut(s) 535
Asp718I GGTACC 1 cut(s) 857
AsuHPI GGTGA 1 cut(s) 751
AxyI CCTNAGG 1 cut(s) 903
BanI GGYRCC 1 cut(s) 857
BanII GRGCYC 2 cut(s) 351, 551
BauI CACGAG 1 cut(s) 930
BbsI GAAGAC 2 cut(s) 512, 910
BbvI GCAGC 1 cut(s) 199
BceAI ACGGC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 429
BcoDI GTCTC 3 cut(s) 376, 626, 816
BfaI CTAG 2 cut(s) 38, 69
BfmI CTRYAG 1 cut(s) 111
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 429
BmeRI GACNNNNNGTC 1 cut(s) 796
BmiI GGNNCC 2 cut(s) 859, 909
BmrFI CCNGG 1 cut(s) 429
BmrI ACTGGG 1 cut(s) 802
BmsI GCATC 1 cut(s) 421
BmuI ACTGGG 1 cut(s) 802
BpiI GAAGAC 2 cut(s) 512, 910
BpmI CTGGAG 3 cut(s) 153, 213, 714
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 500
Bse1I ACTGG 2 cut(s) 731, 797
Bse21I CCTNAGG 1 cut(s) 903
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 165, 500
BseMII CTCAG 1 cut(s) 827
BseNI ACTGG 2 cut(s) 731, 797
BseXI GCAGC 1 cut(s) 199
BseYI CCCAGC 1 cut(s) 170
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 857
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 165, 500
BsmAI GTCTC 3 cut(s) 376, 626, 816
BsmBI CGTCTC 1 cut(s) 816
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 351, 551
Bsp143I GATC 2 cut(s) 516, 895
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 826
BspLI GGNNCC 2 cut(s) 859, 909
BspPI GGATC 1 cut(s) 890
BspT107I GGYRCC 1 cut(s) 857
BsrI ACTGG 2 cut(s) 731, 797
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 516, 895
BssSI CACGAG 1 cut(s) 930
BssT1I CCWWGG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 930
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 448, 559
BstC8I GCNNGC 3 cut(s) 106, 280, 772
BstDEI CTNAG 2 cut(s) 813, 903
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 519, 898
BstMAI GTCTC 3 cut(s) 376, 626, 816
BstMBI GATC 2 cut(s) 516, 895
BstMWI GCNNNNNNNGC 5 cut(s) 209, 218, 346, 409, 941
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 2 cut(s) 512, 910
BstXI CCANNNNNNTGG 1 cut(s) 886
Bsu36I CCTNAGG 1 cut(s) 903
BsuRI GGCC 1 cut(s) 93
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 3 cut(s) 582, 654, 714
BtsIMutI CAGTG 4 cut(s) 280, 582, 654, 714
Cac8I GCNNGC 3 cut(s) 106, 280, 772
CaiI CAGNNNCTG 1 cut(s) 131
CseI GACGC 2 cut(s) 798, 924
Csp6I GTAC 2 cut(s) 455, 858
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 5 cut(s) 26, 341, 416, 636, 781
CviQI GTAC 2 cut(s) 455, 858
DdeI CTNAG 2 cut(s) 813, 903
DpnI GATC 2 cut(s) 518, 897
DpnII GATC 2 cut(s) 516, 895
DraI TTTAAA 2 cut(s) 622, 690
DriI GACNNNNNGTC 1 cut(s) 796
Eam1105I GACNNNNNGTC 1 cut(s) 796
Eco130I CCWWGG 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 351, 551
Eco32I GATATC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 611
Eco81I CCTNAGG 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 427
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 351, 551
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 5 cut(s) 29, 344, 419, 639, 784
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 79
FatI CATG 5 cut(s) 25, 340, 415, 635, 780
FblI GTMKAC 2 cut(s) 441, 735
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 2 cut(s) 351, 551
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 2 cut(s) 38, 69
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 3 cut(s) 153, 213, 714
HaeIII GGCC 1 cut(s) 93
HgaI GACGC 2 cut(s) 798, 924
Hin1II CATG 5 cut(s) 29, 344, 419, 639, 784
HinfI GANTC 3 cut(s) 30, 460, 797
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 3 cut(s) 442, 736, 750
Hpy188I TCNGA 4 cut(s) 591, 678, 816, 940
Hpy188III TCNNGA 4 cut(s) 82, 132, 606, 701
Hpy8I GTNNAC 3 cut(s) 442, 736, 750
HpyAV CCTTC 1 cut(s) 957
HpyCH4III ACNGT 2 cut(s) 448, 559
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 4 cut(s) 221, 308, 340, 647
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 218, 346, 409, 941
HpyF3I CTNAG 2 cut(s) 813, 903
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 5 cut(s) 29, 344, 419, 639, 784
KpnI GGTACC 1 cut(s) 861
Kzo9I GATC 2 cut(s) 516, 895
LmnI GCTCC 3 cut(s) 232, 254, 376
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 421
MaeI CTAG 2 cut(s) 38, 69
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 3 cut(s) 241, 820, 841
MalI GATC 2 cut(s) 518, 897
MboI GATC 2 cut(s) 516, 895
MboII GAAGA 4 cut(s) 39, 97, 517, 910
MhlI GDGCHC 2 cut(s) 351, 551
MluCI AATT 7 cut(s) 205, 234, 391, 485, 531, 617, 827
MlyI GAGTC 2 cut(s) 24, 806
MmeI TCCRAC 1 cut(s) 508
MnlI CCTC 6 cut(s) 104, 128, 290, 485, 921, 939
Mph1103I ATGCAT 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 7 cut(s) 260, 399, 621, 681, 689, 711, 786
MslI CAYNNNNRTG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 5 cut(s) 209, 218, 346, 409, 941
NdeII GATC 2 cut(s) 516, 895
NlaIII CATG 5 cut(s) 29, 344, 419, 639, 784
NlaIV GGNNCC 2 cut(s) 859, 909
NmuCI GTSAC 1 cut(s) 820
NsiI ATGCAT 1 cut(s) 223
PceI AGGCCT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 806
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 460
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 2 cut(s) 24, 805
PpsI GAGTC 2 cut(s) 24, 805
PsiI TTATAA 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 2 cut(s) 859, 909
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 456, 859
RsaNI GTAC 2 cut(s) 455, 858
RseI CAYNNNNRTG 1 cut(s) 560
SaqAI TTAA 7 cut(s) 260, 399, 621, 681, 689, 711, 786
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 516, 895
SchI GAGTC 2 cut(s) 24, 806
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 2 cut(s) 351, 551
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 111
SmiI ATTTAAAT 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 560
Sse9I AATT 7 cut(s) 205, 234, 391, 485, 531, 617, 827
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 1 cut(s) 41
SspI AATATT 1 cut(s) 756
SspMI CTAG 2 cut(s) 38, 69
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 1 cut(s) 249
SwaI ATTTAAAT 1 cut(s) 622
TaaI ACNGT 2 cut(s) 448, 559
TaiI ACGT 1 cut(s) 499
TasI AATT 7 cut(s) 205, 234, 391, 485, 531, 617, 827
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 7 cut(s) 260, 399, 621, 681, 689, 711, 786
Tru9I TTAA 7 cut(s) 260, 399, 621, 681, 689, 711, 786
TscAI CASTG 4 cut(s) 280, 582, 654, 721
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 3 cut(s) 14, 168, 939
TspRI CASTG 4 cut(s) 280, 582, 654, 721
Van91I CCANNNNNTGG 1 cut(s) 500
XapI RAATTY 2 cut(s) 391, 531
XmiI GTMKAC 2 cut(s) 441, 735
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 38, 69
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.