Rw4G015300

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003718
Physical Location & Seq
Forward (+)
35089948 .. 35095298
5351 bp
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UTR
Exon/CDS
Intron
Rw4G015300.1

Sequence Viewer

Length: 984 bp
ATGGGATTGTCGCCTGACAATGTTCATGGACTCACTCTAGCGGTTTCTTCAAGCGTTTTTATTGGGACTAGCTTTATAATCAAGAAGAAAGGCCTCAAAAAAGCAGGCACTACAGGAGTTAGAGCAGGGTCTGGAGGGCATACATACTTGTATGAACCCTTATGGTGGGCTGGGATGATATCAATGATTGTGGGGGAGATAGCTAATTTTGCTGCTTATGCATTTGCTCCAGCAATTCTTGTAACTCCCTTGGGAGCATTAAGTATTATCGTCAGTGCAGTGCTTGCTCATTTTGTTTTGGAGGAAAAACTGCATATATTTGGGGTGCTTGGTTGTGTTCTTTGCATGGTGGGCTCTACAACTATTGTTTTGAATGCTCCACAAGAGAGACAAATAAATTCTGTTAAGCAAGTGTGGCATCATGCTACACAGCCAGGTTTTATTGTCTACACAGTTTTCGTACTGATTCTCGTTGCCGTCCTCATTTTCCAATTTGTTCCACGTTATGGGAAGACCCACCTGATCGTATATGTTGGAATTTGTTCTCTTATGGGCTCTCTCACGGTTATGAGTGTGAAAGCAGTGGGTATCGCTCTGAAGCTGTCCTTTTCAGGAACAAACCAATTTAAATACTATGAGACATGGGTTTTTGCAGTGATAGTGGCAGTCTGTTGTATTTTTCAGATTAACTATTTAAACAAGGCTCTGGACACCTTTAACACTGCTGTAATATCTCCAGTCTACTATGTGATGTTTACAATATTCACCATTCTTGCCAGCGTTATCATGTTTAAGGACTGGGAGTCGCAAAGTGCGTCTCAGATTGTGACTGAATTGTGTGGTTTTGTTACTATCCTATCTGGTACCTTTCTACTTCACAAAACCAGAGATATGGGAAATGATCCACCTAAGGAACCTCCTGTCTTCCAAAGTCCTCGTGCGTCAGAAGCACCTTCATCTCCACTTCCTATTAGCCAGAGATAG

Protein Analysis

327

Amino Acids

35.58

Weight (kDa)

8.96

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 6 - 298 2.1e-138 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 506
AccI GTMKAC 2 cut(s) 447, 741
AciI CCGC 1 cut(s) 41
AclWI GGATC 1 cut(s) 896
AcsI RAATTY 2 cut(s) 397, 537
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 2 cut(s) 462, 865
AfiI CCNNNNNNNGG 2 cut(s) 165, 506
AgsI TTSAA 2 cut(s) 51, 373
AhdI GACNNNNNGTC 1 cut(s) 802
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 3 cut(s) 72, 203, 601
AluI AGCT 3 cut(s) 72, 203, 601
Alw26I GTCTC 3 cut(s) 382, 632, 822
AlwI GGATC 1 cut(s) 896
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 2 cut(s) 397, 537
Asp700I GAANNNNTTC 1 cut(s) 541
Asp718I GGTACC 1 cut(s) 863
AsuHPI GGTGA 1 cut(s) 757
AxyI CCTNAGG 1 cut(s) 909
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 2 cut(s) 356, 557
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 2 cut(s) 518, 916
BbvI GCAGC 1 cut(s) 199
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 1 cut(s) 435
BcoDI GTCTC 3 cut(s) 382, 632, 822
BfaI CTAG 2 cut(s) 38, 69
BfmI CTRYAG 1 cut(s) 111
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 435
BmeRI GACNNNNNGTC 1 cut(s) 802
BmiI GGNNCC 2 cut(s) 865, 915
BmrFI CCNGG 1 cut(s) 435
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 1 cut(s) 427
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 2 cut(s) 518, 916
BpmI CTGGAG 3 cut(s) 153, 213, 720
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 506
Bse1I ACTGG 2 cut(s) 737, 803
Bse21I CCTNAGG 1 cut(s) 909
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 165, 506
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 2 cut(s) 737, 803
BseXI GCAGC 1 cut(s) 199
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 297
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 863
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 165, 506
BsmAI GTCTC 3 cut(s) 382, 632, 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 356, 557
Bsp143I GATC 2 cut(s) 522, 901
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 865, 915
BspPI GGATC 1 cut(s) 896
BspT107I GGYRCC 1 cut(s) 863
BsrI ACTGG 2 cut(s) 737, 803
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 522, 901
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 454, 565
BstAPI GCANNNNNTGC 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 106, 285, 778
BstDEI CTNAG 2 cut(s) 819, 909
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 525, 904
BstMAI GTCTC 3 cut(s) 382, 632, 822
BstMBI GATC 2 cut(s) 522, 901
BstMWI GCNNNNNNNGC 6 cut(s) 209, 218, 284, 351, 415, 947
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 2 cut(s) 518, 916
BstXI CCANNNNNNTGG 1 cut(s) 892
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 1 cut(s) 93
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 4 cut(s) 285, 588, 660, 720
BtsIMutI CAGTG 5 cut(s) 280, 285, 588, 660, 720
Cac8I GCNNGC 3 cut(s) 106, 285, 778
CaiI CAGNNNCTG 1 cut(s) 131
CseI GACGC 2 cut(s) 804, 930
Csp6I GTAC 2 cut(s) 461, 864
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 5 cut(s) 26, 346, 422, 642, 787
CviQI GTAC 2 cut(s) 461, 864
DdeI CTNAG 2 cut(s) 819, 909
DpnI GATC 2 cut(s) 524, 903
DpnII GATC 2 cut(s) 522, 901
DraI TTTAAA 2 cut(s) 628, 696
DriI GACNNNNNGTC 1 cut(s) 802
Eam1105I GACNNNNNGTC 1 cut(s) 802
Eco130I CCWWGG 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 356, 557
Eco32I GATATC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 617
Eco81I CCTNAGG 1 cut(s) 909
EcoRII CCWGG 1 cut(s) 433
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 356, 557
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 5 cut(s) 29, 349, 425, 645, 790
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FaqI GGGAC 1 cut(s) 79
FatI CATG 5 cut(s) 25, 345, 421, 641, 786
FblI GTMKAC 2 cut(s) 447, 741
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 2 cut(s) 356, 557
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 2 cut(s) 38, 69
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 3 cut(s) 153, 213, 720
HaeIII GGCC 1 cut(s) 93
HgaI GACGC 2 cut(s) 804, 930
Hin1II CATG 5 cut(s) 29, 349, 425, 645, 790
HinfI GANTC 3 cut(s) 30, 466, 803
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 3 cut(s) 448, 742, 756
Hpy188I TCNGA 4 cut(s) 597, 684, 822, 946
Hpy188III TCNNGA 4 cut(s) 82, 132, 612, 707
Hpy8I GTNNAC 3 cut(s) 448, 742, 756
HpyAV CCTTC 1 cut(s) 963
HpyCH4III ACNGT 2 cut(s) 454, 565
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 5 cut(s) 221, 278, 313, 345, 653
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 218, 284, 351, 415, 947
HpyF3I CTNAG 2 cut(s) 819, 909
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 5 cut(s) 29, 349, 425, 645, 790
KpnI GGTACC 1 cut(s) 867
Kzo9I GATC 2 cut(s) 522, 901
LmnI GCTCC 3 cut(s) 232, 254, 382
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 427
MaeI CTAG 2 cut(s) 38, 69
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 3 cut(s) 241, 826, 847
MalI GATC 2 cut(s) 524, 903
MboI GATC 2 cut(s) 522, 901
MboII GAAGA 4 cut(s) 39, 97, 523, 916
MhlI GDGCHC 2 cut(s) 356, 557
MluCI AATT 7 cut(s) 205, 234, 397, 491, 537, 623, 833
MlyI GAGTC 2 cut(s) 24, 812
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 6 cut(s) 104, 128, 295, 491, 927, 945
Mph1103I ATGCAT 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 7 cut(s) 260, 405, 627, 687, 695, 717, 792
MslI CAYNNNNRTG 1 cut(s) 566
MspR9I CCNGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 6 cut(s) 209, 218, 284, 351, 415, 947
NdeII GATC 2 cut(s) 522, 901
NlaIII CATG 5 cut(s) 29, 349, 425, 645, 790
NlaIV GGNNCC 2 cut(s) 865, 915
NmuCI GTSAC 1 cut(s) 826
NsiI ATGCAT 1 cut(s) 223
PceI AGGCCT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 812
PctI GAATGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 2 cut(s) 24, 811
PpsI GAGTC 2 cut(s) 24, 811
PsiI TTATAA 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 865, 915
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 462, 865
RsaNI GTAC 2 cut(s) 461, 864
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 7 cut(s) 260, 405, 627, 687, 695, 717, 792
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 522, 901
SchI GAGTC 2 cut(s) 24, 812
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 2 cut(s) 356, 557
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 111
SmiI ATTTAAAT 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 566
Sse9I AATT 7 cut(s) 205, 234, 397, 491, 537, 623, 833
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 1 cut(s) 41
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 38, 69
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 249
SwaI ATTTAAAT 1 cut(s) 628
TaaI ACNGT 2 cut(s) 454, 565
TaiI ACGT 1 cut(s) 505
TasI AATT 7 cut(s) 205, 234, 397, 491, 537, 623, 833
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 7 cut(s) 260, 405, 627, 687, 695, 717, 792
Tru9I TTAA 7 cut(s) 260, 405, 627, 687, 695, 717, 792
TscAI CASTG 5 cut(s) 280, 285, 588, 660, 727
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 3 cut(s) 14, 168, 945
TspRI CASTG 5 cut(s) 280, 285, 588, 660, 727
Van91I CCANNNNNTGG 1 cut(s) 506
XapI RAATTY 2 cut(s) 397, 537
XmiI GTMKAC 2 cut(s) 447, 741
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 2 cut(s) 38, 69
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.