AT3G24110

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008345
Physical Location & Seq
Reverse (-)
8704208 .. 8705550
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24110.1

Sequence Viewer

Length: 699 bp
ATGAGAAACATGCTTTGCTGCTGTGTTAACTCGGAGAACAACAAGAAGTATGCAGAGCTGGATGCGAAGCTTGCACGGAAAATGGTGGAGAGCCGGAGTATTTATCCGGGACACAGGAGTTTAAAGTCTATGGATTCAATAATCATGAAGTTTCCTAAGCTAAGAGAAGGATTAAGAAACATCAGAAGCGTTTTCGAATCATACGATAATGATACAAACGGGACAATCGATATCGAGGAGCTGAAGAAATGTTTAGAGGAACTAAAGCTGAGTTTATCGGATGAAGAAGTGAAAGGTTTATATAGTTGGTGTGACGTTGATGGAAGCAAAGGGATACAATTCAATGAGTTTATAGTCCTTCTCTGTCTCATCTATCTTCTGGCGAAACCTTCCTCTGAATCCAGCACGGAATCGAGAGAGATGGGTCCAAAGTTGGTTGAGTCTATATTTGATCCAATAGTAGAAGTGTTCTTGTTTTTGGACAAAGACGGTAAAGGGAAATTAAACAAGGCTGATGTGATAAAGACATTGAACAACGAAGACTATCCGCTAGAAAGATCTCCTTCACATGTCACCAACATGAGATTTGAAGAAATGGATTGGGGAAGAAAAGGGAAAGTGGGTTTTAGAGAGTTTCTATTTGCTTTCATGAGTTGGGTTGGTCTTGATGATGCAGATGATGATTTTATGAGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_6 EF-hand_7

Protein Analysis

232

Amino Acids

26.67

Weight (kDa)

5.05

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 60 - 118 1.6e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 548
AclWI GGATC 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 4 cut(s) 138, 343, 532, 590
AluBI AGCT 6 cut(s) 58, 70, 160, 241, 268, 696
AluI AGCT 6 cut(s) 58, 70, 160, 241, 268, 696
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 18, 693
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 565
AsuII TTCGAA 1 cut(s) 195
AvaII GGWCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 546
BbvI GCAGC 1 cut(s) 5
BccI CCATC 2 cut(s) 314, 415
BciVI GTATCC 1 cut(s) 327
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 551
BfuI GTATCC 1 cut(s) 327
BglII AGATCT 1 cut(s) 557
BisI GCNGC 2 cut(s) 19, 694
BlsI GCNGC 2 cut(s) 20, 695
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 52, 661
BpiI GAAGAC 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 228
BseGI GGATG 2 cut(s) 67, 286
BseMII CTCAG 1 cut(s) 260
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 228
BsiSI CCGG 2 cut(s) 94, 107
BslFI GGGAC 2 cut(s) 123, 235
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 2 cut(s) 123, 235
Bsp119I TTCGAA 1 cut(s) 195
Bsp143I GATC 2 cut(s) 451, 557
BspACI CCGC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 261
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 2 cut(s) 144, 648
BspLI GGNNCC 1 cut(s) 426
BspPI GGATC 1 cut(s) 446
BspT104I TTCGAA 1 cut(s) 195
BssMI GATC 2 cut(s) 451, 557
Bst4CI ACNGT 1 cut(s) 491
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 3 cut(s) 156, 161, 269
BstF5I GGATG 2 cut(s) 67, 286
BstKTI GATC 2 cut(s) 454, 560
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 2 cut(s) 451, 557
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNSI RCATGY 2 cut(s) 13, 572
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 5
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 1 cut(s) 327
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 2 cut(s) 67, 286
Cac8I GCNNGC 1 cut(s) 72
CciI TCATGA 2 cut(s) 144, 648
Cfr13I GGNCC 1 cut(s) 425
ClaI ATCGAT 1 cut(s) 228
CviAII CATG 5 cut(s) 10, 145, 569, 580, 649
CviJI RGCY 8 cut(s) 58, 70, 93, 160, 241, 268, 512, 696
CviKI_1 RGCY 8 cut(s) 58, 70, 93, 160, 241, 268, 512, 696
DdeI CTNAG 3 cut(s) 156, 161, 269
DpnI GATC 2 cut(s) 453, 559
DpnII GATC 2 cut(s) 451, 557
DraI TTTAAA 1 cut(s) 123
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 263
EcoRV GATATC 1 cut(s) 232
FaeI CATG 5 cut(s) 13, 148, 572, 583, 652
FalI AAGNNNNNCTT 2 cut(s) 547, 579
FaqI GGGAC 2 cut(s) 123, 235
FatI CATG 5 cut(s) 9, 144, 568, 579, 648
Fnu4HI GCNGC 2 cut(s) 19, 694
FokI GGATG 2 cut(s) 74, 293
Fsp4HI GCNGC 2 cut(s) 19, 694
FspBI CTAG 1 cut(s) 551
GluI GCNGC 2 cut(s) 19, 694
HapII CCGG 2 cut(s) 94, 107
Hin1II CATG 5 cut(s) 13, 148, 572, 583, 652
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 5 cut(s) 134, 197, 398, 410, 440
HpaI GTTAAC 1 cut(s) 28
HpaII CCGG 2 cut(s) 94, 107
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 34, 185, 280, 397
Hpy188III TCNNGA 4 cut(s) 145, 414, 649, 665
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 161, 368, 399, 573
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 3 cut(s) 53, 74, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 3 cut(s) 156, 161, 269
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 5 cut(s) 13, 148, 572, 583, 652
KspAI GTTAAC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 451, 557
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 6 cut(s) 44, 100, 107, 120, 365, 415
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 2 cut(s) 52, 661
MaeI CTAG 1 cut(s) 551
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 311, 571
MalI GATC 2 cut(s) 453, 559
MboI GATC 2 cut(s) 451, 557
MboII GAAGA 6 cut(s) 256, 296, 368, 551, 602, 618
MflI RGATCY 1 cut(s) 557
MluCI AATT 2 cut(s) 338, 500
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 3 cut(s) 229, 250, 403
MseI TTAA 4 cut(s) 27, 122, 173, 503
MslI CAYNNNNRTG 1 cut(s) 578
MspI CCGG 2 cut(s) 94, 107
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 108
NdeII GATC 2 cut(s) 451, 557
NlaIII CATG 5 cut(s) 13, 148, 572, 583, 652
NlaIV GGNNCC 1 cut(s) 426
NmuCI GTSAC 2 cut(s) 311, 571
NspI RCATGY 2 cut(s) 13, 572
NspV TTCGAA 1 cut(s) 195
PagI TCATGA 2 cut(s) 144, 648
PciI ACATGT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 225
PfeI GAWTC 4 cut(s) 134, 197, 398, 410
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 2 cut(s) 20, 695
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 568
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 1 cut(s) 425
PsuI RGATCY 1 cut(s) 557
RseI CAYNNNNRTG 1 cut(s) 578
SaqAI TTAA 4 cut(s) 27, 122, 173, 503
SatI GCNGC 2 cut(s) 19, 694
Sau3AI GATC 2 cut(s) 451, 557
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 108
SetI ASST 9 cut(s) 60, 72, 162, 243, 270, 298, 318, 391, 698
SfaNI GCATC 2 cut(s) 52, 661
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 578
Sse9I AATT 2 cut(s) 338, 500
SsiI CCGC 1 cut(s) 548
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 1 cut(s) 318
TaqI TCGA 4 cut(s) 195, 228, 234, 413
TasI AATT 2 cut(s) 338, 500
TfiI GAWTC 4 cut(s) 134, 197, 398, 410
Tru1I TTAA 4 cut(s) 27, 122, 173, 503
Tru9I TTAA 4 cut(s) 27, 122, 173, 503
TseFI GTSAC 2 cut(s) 311, 571
TseI GCWGC 2 cut(s) 18, 693
Tsp45I GTSAC 2 cut(s) 311, 571
TspDTI ATGAA 3 cut(s) 161, 297, 637
TspGWI ACGGA 2 cut(s) 91, 422
VpaK11BI GGWCC 1 cut(s) 425
XceI RCATGY 2 cut(s) 13, 572
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.