FvH4_4g15760

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008345
Physical Location & Seq
Reverse (-)
19657068 .. 19660605
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15760.t1

Sequence Viewer

Length: 732 bp
ATGAAGGCTTCGGGCACATCTCATCTTTGCTGTTCCGTGAAGACTTTGTCAGACAAAGTAGGAGGCATGCTTTGTTGTCGTGGTTCTGCGAACAAGTATAGCAGGCTAGATGCAAAGCTTGAAAAGAGAATGGCTGAATTTAAAAGAGTTTCTTCGGGCCAAAGCAACTTTAGGTCAATTGACAGCATAATTTTGAGGTTCCCTCAGTTTAGAGAAGAACTGAAGAACATAAGGAACGTGTTTGACTTGTATGATGAGGACTCAAATGGAACTATAGACCGCGAGGAACTGAAGAAATGCTTACAGAAACTCCAACTCCATCTGACAGCGGAAGAGATTGAGGATCTTTTTCATTCATGTGACATGGATGGGAGTGAAGGGATACAATTCAATGAATTCATTGTTCTTCTATGTCTCATATATCTATTGAAGGACCCCAATACGACATCGAAGATAGACTGTCCACAACTAGAAGCAACTTTTGATACTGTCATTGAAGTATTTGTATTTCTCGATAAAAATGGTGATGGAAAGCTGAACAAGACAGAAGTGGTGAAGGCATTGAATGAAGCTTCTCCACGGGAGAAATCTCCTTCTCATGTCAGCAGGAATCGATTTAAGGAAATGGATTGGGACAAGAATGGGAAGGTGACTTTCAGGCAGTTCCTCTTTGCTTTCATCGATTGGGTCGGAATTGAAACAGATGAAGAAATATCTCAAGAACAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

244

Amino Acids

27.88

Weight (kDa)

5.3

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 76 - 134 1.1e-11 EF-hand domain pair
EF-hand_1 PF00036 77 - 104 1.4e-06 EF hand domain
EF-hand_6 PF13405 77 - 104 1.8e-07 EF-hand domain
EF-hand_8 PF13833 88 - 137 3.7e-09 EF-hand domain pair
EF-hand_7 PF13499 164 - 225 1.8e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 2 cut(s) 280, 329
AclWI GGATC 1 cut(s) 351
AcsI RAATTY 2 cut(s) 137, 395
AcuI CTGAAG 2 cut(s) 242, 311
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 6 cut(s) 122, 391, 430, 497, 565, 698
AluBI AGCT 3 cut(s) 118, 535, 572
AluI AGCT 3 cut(s) 118, 535, 572
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 1 cut(s) 351
AoxI GGCC 1 cut(s) 157
ApoI RAATTY 2 cut(s) 137, 395
AspS9I GGNCC 2 cut(s) 157, 433
AsuHPI GGTGA 3 cut(s) 536, 565, 661
AvaII GGWCC 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 47
BccI CCATC 3 cut(s) 327, 362, 521
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 107, 470
BfmI CTRYAG 1 cut(s) 273
BfuI GTATCC 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 157, 433
BmiI GGNNCC 2 cut(s) 200, 435
BmsI GCATC 1 cut(s) 100
BpiI GAAGAC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 187, 219
BpuEI CTTGAG 1 cut(s) 702
Bsa29I ATCGAT 2 cut(s) 613, 681
BsaJI CCNNGG 1 cut(s) 578
BsaXI ACNNNNNCTCC 4 cut(s) 294, 300, 324, 330
BseCI ATCGAT 2 cut(s) 613, 681
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 1 cut(s) 159
BshVI ATCGAT 2 cut(s) 613, 681
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 2 cut(s) 280, 329
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 2 cut(s) 613, 681
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 200, 435
BspPI GGATC 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 461, 490
Bst6I CTCTTC 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 68, 104
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 1 cut(s) 373
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
Bsu15I ATCGAT 2 cut(s) 613, 681
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 159
BsuTUI ATCGAT 2 cut(s) 613, 681
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 1 cut(s) 373
Cac8I GCNNGC 2 cut(s) 68, 104
Cfr13I GGNCC 2 cut(s) 157, 433
ClaI ATCGAT 2 cut(s) 613, 681
CviAII CATG 4 cut(s) 67, 357, 364, 599
CviJI RGCY 7 cut(s) 8, 106, 118, 134, 159, 535, 572
CviKI_1 RGCY 7 cut(s) 8, 106, 118, 134, 159, 535, 572
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
DraI TTTAAA 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 242, 311
EcoO109I RGGNCCY 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 395
FaeI CATG 4 cut(s) 70, 360, 367, 602
FalI AAGNNNNNCTT 4 cut(s) 136, 168, 284, 316
FaqI GGGAC 1 cut(s) 647
FatI CATG 4 cut(s) 66, 356, 363, 598
FokI GGATG 1 cut(s) 380
FspBI CTAG 2 cut(s) 107, 470
HaeIII GGCC 1 cut(s) 159
Hin1II CATG 4 cut(s) 70, 360, 367, 602
HindIII AAGCTT 2 cut(s) 116, 570
HinfI GANTC 2 cut(s) 260, 610
HphI GGTGA 3 cut(s) 536, 565, 661
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 3 cut(s) 52, 324, 692
Hpy188III TCNNGA 2 cut(s) 512, 719
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 5 cut(s) 371, 424, 550, 603, 640
HpyCH4III ACNGT 2 cut(s) 461, 490
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 4 cut(s) 70, 360, 367, 602
Kzo9I GATC 1 cut(s) 343
LpnPI CCDG 3 cut(s) 88, 592, 643
LweI GCATC 1 cut(s) 100
MaeI CTAG 2 cut(s) 107, 470
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 359, 649
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 9 cut(s) 52, 144, 227, 235, 304, 344, 398, 463, 719
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 6 cut(s) 137, 177, 189, 386, 395, 693
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 337, 670
MnlI CCTC 7 cut(s) 56, 189, 213, 250, 277, 334, 677
MseI TTAA 3 cut(s) 141, 618, 730
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 329
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 282
NdeII GATC 1 cut(s) 343
NlaIII CATG 4 cut(s) 70, 360, 367, 602
NlaIV GGNNCC 2 cut(s) 200, 435
NmuCI GTSAC 2 cut(s) 359, 649
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 610
PflFI GACNNNGTC 1 cut(s) 46
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 200, 435
PspPI GGNCC 2 cut(s) 157, 433
PspPPI RGGWCCY 1 cut(s) 433
PsuI RGATCY 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 3 cut(s) 141, 618, 730
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 2 cut(s) 157, 433
SchI GAGTC 1 cut(s) 254
SduI GDGCHC 1 cut(s) 17
SetI ASST 7 cut(s) 120, 176, 200, 240, 537, 574, 651
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
SphI GCATGC 1 cut(s) 70
Sse9I AATT 6 cut(s) 137, 177, 189, 386, 395, 693
SsiI CCGC 2 cut(s) 280, 329
SspMI CTAG 2 cut(s) 107, 470
TaaI ACNGT 2 cut(s) 461, 490
TaiI ACGT 1 cut(s) 240
TaqI TCGA 4 cut(s) 449, 513, 613, 681
TasI AATT 6 cut(s) 137, 177, 189, 386, 395, 693
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 3 cut(s) 141, 618, 730
Tru9I TTAA 3 cut(s) 141, 618, 730
TseFI GTSAC 2 cut(s) 359, 649
Tsp45I GTSAC 2 cut(s) 359, 649
TspDTI ATGAA 8 cut(s) 17, 341, 345, 388, 408, 582, 667, 720
TspGWI ACGGA 1 cut(s) 25
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 2 cut(s) 137, 395
XceI RCATGY 1 cut(s) 70
XspI CTAG 2 cut(s) 107, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.