Rorug04G0133700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008345
Physical Location & Seq
Reverse (-)
21514839 .. 21520192
5354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0133700.1

Sequence Viewer

Length: 1965 bp
ATGGCAGCAGGCGCAGGCAGTCAGCAAAGCTTGAGAAAAGCGTTGAAAACAGTTGGATTGGCGAAAGCCAATGGTGAATTTAAGGTATTGGATAATGCCATTTCCAAGGCCACAAGACATGATGACGCATTGCCAAAGGAGAAACATGTTACTACTGTACTACGTGCAATTCCAGCTTCAAGACCTCGTGCTGAAGTTGCTTATTGCATACATGCTCTAGCCAGGCGCCTTTCTAAGGCTCACAGTTGGAAGGTAGCACTGAAAACCGTGATGATTATACACCGTGCTCTGAGGGAAGTTGATGATACATTTTGTGACGCTCTAATTAAGTATAGCTGGAGTAGAGGTCATATTCTGAACATATCTCATTCGTGGGATGAAACAAGTCCAAGTGCATGGGGTTATTCTACCTGGGTTCGTTTATATGCATCATATCTCGAAGAACGCCTGGAGTGTTTTCGTGTCCTCAAGTATGATGTTCAGAAAGATCACTTGAGAACCAAGGAACTTGAAACCCCAGACTTACTAAGGCATCTACCTGCCTTGCAACAGCTTCTTTCTCGCCTTCTTGATTGCCAGCCAGAAGTGGGGACTATATGTAATAGCCTGATTCAGTATGCTATTTCAATGGTTGCAGGTGAAAGTGCCAAGTTGTATGCTGCAATTACAGATGGTATTGTGAATTTAGTTGACAAGTTCTTTGAGATGCAGCGCGATGATGCAGTTAAAGCACTTGAAATTTATAAGAAGTCGGGAAGCCAGGCAGAGAGGTTATTTGAGTTCTTTGAGATCTGCAGGAGCCTTAATTTTGGACGGGGTCTGAAAACGGTTAGAATTCAACAGCTCCCTGAATCATTCTTGACCACTATGGAAGATTATGTTAAGGAGGCATCCGGTGTTGTAATGCTTGAACTTCCCCCAACCATTGAGGATCAAACTGATGCTCCCAAGGAAACTGTTGTGGTAGAAGGGGACTTGTTAATCGACCATGATGATAATAAGGAACAAAAATCAGCACCTCAACATCCAAGTGACCAGAGTGACGCTGCTGCGACAACACAAATTTTTGACCTGCTGAGCTTTGATGAAGTTATTCCAGTAACATCAGAGTCAGATGAAAACAATTCCCTGGCACTAGCAATCGTTCCGTCGGAGAATCCGTCAGATTCCAAAACAGATGGTCTCAATTCTACAACCTCCAACTCTAGTTGGGAGATTGAACTTTTTACACCAACTTCTAATGGAGCTGCAGTTCAAGAGACACCATCAACTTCTAATGCAGCTGGAGTTGCAGAAACCAAGTTGGCTGGTGGGCTAGACAGATCGACACTGGACAGTCTGTATGATAATGCAATGGCAAGTACACCAAATCAGAACAACATGTTGTACTCCCCAGCCGCACTGGCACCCTCTAATCCTTTTGAAGATGTTGCTACTTCTTCATACCAATTCCAAGACCCTTATTATGCTTCCAGCAATGCAACAACCCCAGCAAACTTACAAATGGCTATCATGACCCAACAACAACAATATTTCATGATGATGCAGCAACAACAAGTGCAGCAACTACAGTCACCCAGCATTGTCCAAAATCCACCGAGTCCTTACCAAAATCTACCGAGTCCTTGCCACAATCCACCAAGTCCTTTCCAAAATTTACCAAATCCTTTCCAAAACCCACCAACACCAAGTCCTAGCCAAAATCCACCAAGTCCTTTCCAAAATTTACCAAATCCTTTCCAAAATTCACCAACACCTAGTCCTAGCCAAAATCCACCAACACCAAGTCCTTTCCAAAATTTACCAAATCCTTTCCAAAATCCACCAGCATCAAGTCCTAACCAAAATCCACCAACACCAAGTCCTTTCCAAAATCCACAAAGTCCATTAACCTCTTCTGGGAATCCTTTTTCTGGGCAGATCAGTAATCCTCCACCTCAAGATTCACACAATGGCCTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

654

Amino Acids

71.94

Weight (kDa)

5.08

Isoelectric Point (pI)

58.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 30 - 295 5.6e-71 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AarI CACCTGC 1 cut(s) 626
Acc36I ACCTGC 3 cut(s) 547, 626, 1080
AccB1I GGYRCC 2 cut(s) 225, 1405
AccII CGCG 1 cut(s) 714
AciI CCGC 1 cut(s) 1398
AclWI GGATC 1 cut(s) 939
AcsI RAATTY 9 cut(s) 77, 682, 738, 834, 1062, 1654, 1723, 1744, 1798
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 3 cut(s) 159, 1363, 1388
AfiI CCNNNNNNNGG 4 cut(s) 235, 587, 1899, 1913
AflIII ACRYGT 2 cut(s) 145, 1380
AjnI CCWGG 5 cut(s) 221, 410, 447, 759, 1128
AloI GAACNNNNNNTCC 2 cut(s) 1236, 1268
AluBI AGCT 8 cut(s) 30, 176, 336, 553, 844, 1080, 1247, 1283
AluI AGCT 8 cut(s) 30, 176, 336, 553, 844, 1080, 1247, 1283
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 2 cut(s) 1187, 1253
AlwI GGATC 1 cut(s) 939
AoxI GGCC 2 cut(s) 108, 1954
ApeKI GCWGC 9 cut(s) 5, 659, 709, 1046, 1049, 1247, 1280, 1546, 1561
ApoI RAATTY 9 cut(s) 77, 682, 738, 834, 1062, 1654, 1723, 1744, 1798
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
Asp700I GAANNNNTTC 1 cut(s) 1092
AspLEI GCGC 3 cut(s) 14, 228, 714
AsuHPI GGTGA 4 cut(s) 86, 650, 1566, 1740
BanI GGYRCC 2 cut(s) 225, 1405
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 9 cut(s) 17, 646, 721, 1033, 1036, 1234, 1292, 1558, 1573
BccI CCATC 3 cut(s) 665, 1172, 1273
BciT130I CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BcoDI GTCTC 2 cut(s) 1187, 1253
BfaI CTAG 7 cut(s) 218, 1136, 1206, 1316, 1695, 1758, 1764
BfmI CTRYAG 3 cut(s) 793, 1248, 1568
BfoI RGCGCY 1 cut(s) 229
BfuAI ACCTGC 3 cut(s) 547, 626, 1080
BglI GCCNNNNNGGC 1 cut(s) 1403
BglII AGATCT 1 cut(s) 789
BlpI GCTNAGC 1 cut(s) 1076
Bme1390I CCNGG 5 cut(s) 223, 412, 449, 761, 1130
BmiI GGNNCC 3 cut(s) 227, 800, 1407
BmrFI CCNGG 5 cut(s) 223, 412, 449, 761, 1130
BmsI GCATC 8 cut(s) 437, 541, 696, 709, 899, 931, 1533, 1838
BpmI CTGGAG 3 cut(s) 358, 470, 1305
Bpu1102I GCTNAGC 1 cut(s) 1076
BpuEI CTTGAG 4 cut(s) 52, 452, 514, 1923
BsaAI YACGTR 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 1187
BsaJI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 8 cut(s) 331, 361, 928, 958, 1145, 1175, 1236, 1266
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 587, 1899, 1913
Bse1I ACTGG 3 cut(s) 1097, 1335, 1407
Bse3DI GCAATG 3 cut(s) 128, 1359, 1483
BseBI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BseDI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BseGI GGATG 3 cut(s) 382, 890, 1024
BseLI CCNNNNNNNGG 4 cut(s) 235, 587, 1899, 1913
BseMI GCAATG 3 cut(s) 128, 1359, 1483
BseMII CTCAG 2 cut(s) 281, 1067
BseNI ACTGG 3 cut(s) 1097, 1335, 1407
BseXI GCAGC 9 cut(s) 17, 646, 721, 1033, 1036, 1234, 1292, 1558, 1573
BseYI CCCAGC 3 cut(s) 1393, 1489, 1577
BsgI GTGCAG 1 cut(s) 1580
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 2 cut(s) 110, 1956
BshNI GGYRCC 2 cut(s) 225, 1405
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 2 cut(s) 604, 986
BslI CCNNNNNNNGG 4 cut(s) 235, 587, 1899, 1913
BsmAI GTCTC 2 cut(s) 1187, 1253
BsmFI GGGAC 2 cut(s) 604, 986
BsnI GGCC 2 cut(s) 110, 1956
Bso31I GGTCTC 1 cut(s) 1187
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 5 cut(s) 487, 789, 931, 1322, 1920
Bsp1720I GCTNAGC 1 cut(s) 1076
BspACI CCGC 1 cut(s) 1398
BspANI GGCC 2 cut(s) 110, 1956
BspCNI CTCAG 2 cut(s) 282, 1068
BspFNI CGCG 1 cut(s) 714
BspHI TCATGA 2 cut(s) 1512, 1536
BspLI GGNNCC 3 cut(s) 227, 800, 1407
BspMAI CTGCAG 2 cut(s) 797, 1252
BspMI ACCTGC 3 cut(s) 547, 626, 1080
BspPI GGATC 1 cut(s) 939
BspT107I GGYRCC 2 cut(s) 225, 1405
BspTNI GGTCTC 1 cut(s) 1187
BsrDI GCAATG 3 cut(s) 128, 1359, 1483
BsrI ACTGG 3 cut(s) 1097, 1335, 1407
BssECI CCNNGG 5 cut(s) 105, 411, 501, 948, 1128
BssMI GATC 5 cut(s) 487, 789, 931, 1322, 1920
BssNI GRCGYC 1 cut(s) 226
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 105, 501, 948
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
Bst4CI ACNGT 9 cut(s) 52, 157, 245, 268, 284, 829, 958, 1337, 1572
Bst6I CTCTTC 1 cut(s) 1900
BstACI GRCGYC 1 cut(s) 226
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 3 cut(s) 10, 16, 578
BstDEI CTNAG 4 cut(s) 234, 290, 527, 1076
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 3 cut(s) 382, 890, 1024
BstFNI CGCG 1 cut(s) 714
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 3 cut(s) 14, 228, 714
BstKTI GATC 5 cut(s) 490, 792, 934, 1325, 1923
BstMAI GTCTC 2 cut(s) 1187, 1253
BstMBI GATC 5 cut(s) 487, 789, 931, 1322, 1920
BstMWI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1403
BstNI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
BstNSI RCATGY 3 cut(s) 149, 215, 1384
BstSCI CCNGG 5 cut(s) 221, 410, 447, 759, 1128
BstSFI CTRYAG 3 cut(s) 793, 1248, 1568
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 9 cut(s) 17, 646, 721, 1033, 1036, 1234, 1292, 1558, 1573
BstX2I RGATCY 1 cut(s) 789
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 1 cut(s) 789
BsuRI GGCC 2 cut(s) 110, 1956
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 3 cut(s) 382, 890, 1024
BtsIMutI CAGTG 3 cut(s) 257, 1328, 1400
BveI ACCTGC 3 cut(s) 547, 626, 1080
Cac8I GCNNGC 3 cut(s) 10, 16, 578
CciI TCATGA 2 cut(s) 1512, 1536
CfoI GCGC 3 cut(s) 14, 228, 714
CseI GACGC 3 cut(s) 134, 326, 1052
Csp6I GTAC 3 cut(s) 158, 1362, 1387
CviAII CATG 8 cut(s) 119, 146, 212, 396, 989, 1381, 1513, 1537
CviQI GTAC 3 cut(s) 158, 1362, 1387
DdeI CTNAG 4 cut(s) 234, 290, 527, 1076
DinI GGCGCC 1 cut(s) 227
DpnI GATC 5 cut(s) 489, 791, 933, 1324, 1922
DpnII GATC 5 cut(s) 487, 789, 931, 1322, 1920
Eam1104I CTCTTC 1 cut(s) 1900
EarI CTCTTC 1 cut(s) 1900
Eco130I CCWWGG 3 cut(s) 105, 501, 948
Eco31I GGTCTC 1 cut(s) 1187
Eco57I CTGAAG 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 834
EcoRII CCWGG 5 cut(s) 221, 410, 447, 759, 1128
EcoT14I CCWWGG 3 cut(s) 105, 501, 948
EcoT22I ATGCAT 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 227
EheI GGCGCC 1 cut(s) 227
ErhI CCWWGG 3 cut(s) 105, 501, 948
FaeI CATG 8 cut(s) 122, 149, 215, 399, 992, 1384, 1516, 1540
FaqI GGGAC 2 cut(s) 604, 986
FatI CATG 8 cut(s) 118, 145, 211, 395, 988, 1380, 1512, 1536
FokI GGATG 3 cut(s) 389, 877, 1011
FspBI CTAG 7 cut(s) 218, 1136, 1206, 1316, 1695, 1758, 1764
GlaI GCGC 3 cut(s) 13, 227, 713
GsaI CCCAGC 3 cut(s) 1397, 1493, 1581
GsuI CTGGAG 3 cut(s) 358, 470, 1305
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 110, 1956
HapII CCGG 1 cut(s) 894
HgaI GACGC 3 cut(s) 134, 326, 1052
HhaI GCGC 3 cut(s) 14, 228, 714
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 8 cut(s) 122, 149, 215, 399, 992, 1384, 1516, 1540
Hin6I GCGC 3 cut(s) 12, 226, 712
HinP1I GCGC 3 cut(s) 12, 226, 712
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 9 cut(s) 610, 851, 1109, 1156, 1166, 1600, 1621, 1903, 1943
HpaII CCGG 1 cut(s) 894
HphI GGTGA 4 cut(s) 86, 650, 1566, 1740
Hpy166II GTNNAC 2 cut(s) 691, 1364
Hpy188I TCNGA 9 cut(s) 291, 357, 483, 822, 1108, 1114, 1153, 1165, 1374
Hpy188III TCNNGA 9 cut(s) 180, 437, 569, 753, 859, 1256, 1513, 1537, 1940
Hpy8I GTNNAC 2 cut(s) 691, 1364
Hpy99I CGWCG 1 cut(s) 1153
HpyAV CCTTC 3 cut(s) 244, 575, 962
HpyCH4III ACNGT 9 cut(s) 52, 157, 245, 268, 284, 829, 958, 1337, 1572
HpyCH4IV ACGT 1 cut(s) 163
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1403
HpyF3I CTNAG 4 cut(s) 234, 290, 527, 1076
HpySE526I ACGT 1 cut(s) 163
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 8 cut(s) 122, 149, 215, 399, 992, 1384, 1516, 1540
HspAI GCGC 3 cut(s) 12, 226, 712
KasI GGCGCC 1 cut(s) 225
Kzo9I GATC 5 cut(s) 487, 789, 931, 1322, 1920
LmnI GCTCC 4 cut(s) 798, 849, 949, 1244
Lsp1109I GCAGC 9 cut(s) 17, 646, 721, 1033, 1036, 1234, 1292, 1558, 1573
LweI GCATC 8 cut(s) 437, 541, 696, 709, 899, 931, 1533, 1838
MaeI CTAG 7 cut(s) 218, 1136, 1206, 1316, 1695, 1758, 1764
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 6 cut(s) 148, 314, 1031, 1040, 1099, 1572
MalI GATC 5 cut(s) 489, 791, 933, 1324, 1922
MboI GATC 5 cut(s) 487, 789, 931, 1322, 1920
MboII GAAGA 5 cut(s) 452, 884, 1431, 1436, 1887
MflI RGATCY 1 cut(s) 789
MhlI GDGCHC 1 cut(s) 289
Mly113I GGCGCC 1 cut(s) 226
MlyI GAGTC 3 cut(s) 1118, 1609, 1630
MmeI TCCRAC 4 cut(s) 34, 227, 1131, 1224
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 7 cut(s) 81, 327, 726, 804, 882, 980, 1889
MslI CAYNNNNRTG 2 cut(s) 1029, 1541
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 5 cut(s) 223, 412, 449, 761, 1130
MvaI CCWGG 5 cut(s) 223, 412, 449, 761, 1130
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 6 cut(s) 11, 173, 197, 728, 1289, 1403
NarI GGCGCC 1 cut(s) 226
NdeII GATC 5 cut(s) 487, 789, 931, 1322, 1920
NlaIII CATG 8 cut(s) 122, 149, 215, 399, 992, 1384, 1516, 1540
NlaIV GGNNCC 3 cut(s) 227, 800, 1407
NmuCI GTSAC 4 cut(s) 314, 1031, 1040, 1572
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 3 cut(s) 149, 215, 1384
PagI TCATGA 2 cut(s) 1512, 1536
PaqCI CACCTGC 1 cut(s) 626
PciI ACATGT 2 cut(s) 145, 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 1157
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 6 cut(s) 610, 851, 1156, 1166, 1903, 1943
PflFI GACNNNGTC 1 cut(s) 816
PleI GAGTC 3 cut(s) 1117, 1608, 1629
PluTI GGCGCC 1 cut(s) 229
PpsI GAGTC 3 cut(s) 1117, 1608, 1629
Ppu21I YACGTR 1 cut(s) 164
PscI ACATGT 2 cut(s) 145, 1380
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 5 cut(s) 221, 410, 447, 759, 1128
PspFI CCCAGC 3 cut(s) 1393, 1489, 1577
PspGI CCWGG 5 cut(s) 221, 410, 447, 759, 1128
PspN4I GGNNCC 3 cut(s) 227, 800, 1407
PstI CTGCAG 2 cut(s) 797, 1252
PsuI RGATCY 1 cut(s) 789
PsyI GACNNNGTC 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 159, 1363, 1388
RsaNI GTAC 3 cut(s) 158, 1362, 1387
RseI CAYNNNNRTG 2 cut(s) 1029, 1541
SaqAI TTAA 7 cut(s) 81, 327, 726, 804, 882, 980, 1889
Sau3AI GATC 5 cut(s) 487, 789, 931, 1322, 1920
SchI GAGTC 3 cut(s) 1118, 1609, 1630
ScrFI CCNGG 5 cut(s) 223, 412, 449, 761, 1130
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 8 cut(s) 437, 541, 696, 709, 899, 931, 1533, 1838
SfcI CTRYAG 3 cut(s) 793, 1248, 1568
SfoI GGCGCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 1029, 1541
SmlI CTYRAG 4 cut(s) 31, 467, 493, 1938
SmoI CTYRAG 4 cut(s) 31, 467, 493, 1938
SsiI CCGC 1 cut(s) 1398
SspDI GGCGCC 1 cut(s) 225
SspI AATATT 1 cut(s) 1532
SspMI CTAG 7 cut(s) 218, 1136, 1206, 1316, 1695, 1758, 1764
StyD4I CCNGG 5 cut(s) 221, 410, 447, 759, 1128
StyI CCWWGG 3 cut(s) 105, 501, 948
TaaI ACNGT 9 cut(s) 52, 157, 245, 268, 284, 829, 958, 1337, 1572
TaiI ACGT 1 cut(s) 166
TaqI TCGA 3 cut(s) 438, 984, 1325
TatI WGTACW 3 cut(s) 157, 1361, 1386
TauI GCSGC 1 cut(s) 1400
TfiI GAWTC 6 cut(s) 610, 851, 1156, 1166, 1903, 1943
Tru1I TTAA 7 cut(s) 81, 327, 726, 804, 882, 980, 1889
Tru9I TTAA 7 cut(s) 81, 327, 726, 804, 882, 980, 1889
TscAI CASTG 3 cut(s) 264, 1335, 1407
TseFI GTSAC 4 cut(s) 314, 1031, 1040, 1572
TseI GCWGC 9 cut(s) 5, 659, 709, 1046, 1049, 1247, 1280, 1546, 1561
Tsp45I GTSAC 4 cut(s) 314, 1031, 1040, 1572
TspDTI ATGAA 5 cut(s) 393, 1101, 1131, 1431, 1525
TspGWI ACGGA 2 cut(s) 1137, 1149
TspRI CASTG 3 cut(s) 264, 1335, 1407
Tth111I GACNNNGTC 1 cut(s) 816
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 9 cut(s) 77, 682, 738, 834, 1062, 1654, 1723, 1744, 1798
XceI RCATGY 3 cut(s) 149, 215, 1384
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 7 cut(s) 218, 1136, 1206, 1316, 1695, 1758, 1764
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.