pycom04g00630

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008345
Physical Location & Seq
Forward (+)
605281 .. 607000
1720 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00630.1

Sequence Viewer

Length: 762 bp
ATGAAGACTTCTTCTCCTTCAGCTGGCACATCCCATCTCCGCTCTCAGCTCAAGTCTATGTCAAACAAAGTAGGAGGTATGCTGTGTTGTTGTAGTTCACCAAATAAGTATAGCAGGTTGGATGCAAAGCTTGAAAAGAAGATGGTTGAAGTTAAAAGAGGTTACTTGGGCCGTAGTAACTTTAAGTCAATTGACAGCATTATCATGAGGTTCCCCCAGTTCAGGGAGGAACTCAAGAAAATTCGAGATGTGTTTGAACAGTATGATGAGGACTCAAATGGAACTATTGACCGTGAAGAACTGAAGAAATGCTTGCAGAAACTGCAACTCCATCTGACAGCGGAGGAGATTGAGGATCTTTTCCATTCATGTGACATGGATGGAAGTGAGGGGATACAATTTAATGAATTCATTGTTCTTCTATGCCTCATCTATCTATTAAAGGAACCTTCATCTTCTCATACGATATCCAAGATAAACTCGCCACAGCTTGAAGCAACTTTTGACACTGTAGTTGAAGTATTCTCATTTCTGGATAAAAATGGCGATGGGAAACTGAACAAGGCAGACATGGTGAAGGCATTGAACGAGGCTTCTCCTTTGGAGAAATCCCCTGCACATGTCACCAGGACTCGATTCAAAGAAATGGATTGGGACAAAAATGGGAAGGTCACTTTCAGGGAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGACACAGATGAAGAAATGTCTCCAGTACGAAGTTCAAGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

254

Amino Acids

28.89

Weight (kDa)

6.03

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 79 - 138 8.7e-12 EF-hand domain pair
EF-hand_6 PF13405 80 - 108 1.2e-07 EF-hand domain
EF-hand_1 PF00036 81 - 108 1.3e-06 EF hand domain
EF-hand_5 PF13202 81 - 105 3e-06 EF hand
EF-hand_8 PF13833 92 - 141 3.9e-09 EF-hand domain pair
EF-hand_7 PF13499 173 - 233 3.6e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 105
AccBSI CCGCTC 1 cut(s) 42
AciI CCGC 2 cut(s) 40, 341
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 2 cut(s) 240, 407
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 3 cut(s) 23, 222, 223
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 8 cut(s) 134, 149, 257, 494, 518, 586, 640, 753
AjnI CCWGG 1 cut(s) 626
AluBI AGCT 4 cut(s) 23, 49, 130, 490
AluI AGCT 4 cut(s) 23, 49, 130, 490
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 1 cut(s) 169
ApoI RAATTY 2 cut(s) 240, 407
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 90, 586, 616
BbsI GAAGAC 1 cut(s) 11
BccI CCATC 5 cut(s) 42, 136, 339, 374, 542
BceAI ACGGC 1 cut(s) 156
BciT130I CCWGG 1 cut(s) 628
BciVI GTATCC 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 387
Bme1390I CCNGG 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 212, 447
BmrFI CCNGG 1 cut(s) 628
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 723
BpuEI CTTGAG 2 cut(s) 35, 218
Bsa29I ATCGAT 1 cut(s) 702
BsaXI ACNNNNNCTCC 3 cut(s) 28, 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 222, 223
Bse1I ACTGG 2 cut(s) 217, 740
BseBI CCWGG 1 cut(s) 628
BseCI ATCGAT 1 cut(s) 702
BseGI GGATG 3 cut(s) 29, 127, 385
BseLI CCNNNNNNNGG 3 cut(s) 23, 222, 223
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 2 cut(s) 217, 740
BseRI GAGGAG 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 702
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 3 cut(s) 23, 222, 223
BsmAI GTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 1 cut(s) 355
BspACI CCGC 2 cut(s) 40, 341
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 1 cut(s) 58
BspDI ATCGAT 1 cut(s) 702
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 212, 447
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 1 cut(s) 363
BsrBI CCGCTC 1 cut(s) 42
BsrI ACTGG 2 cut(s) 217, 740
BssMI GATC 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 3 cut(s) 261, 293, 511
BstAPI GCANNNNNTGC 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 25, 314
BstDEI CTNAG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 29, 127, 385
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 322
BstNI CCWGG 1 cut(s) 628
BstNSI RCATGY 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 626
BstSFI CTRYAG 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu15I ATCGAT 1 cut(s) 702
BsuI GTATCC 1 cut(s) 387
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 3 cut(s) 29, 127, 385
BtsIMutI CAGTG 1 cut(s) 507
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 25, 314
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 702
Csp6I GTAC 1 cut(s) 743
CviAII CATG 5 cut(s) 205, 369, 376, 571, 620
CviJI RGCY 6 cut(s) 23, 49, 130, 171, 490, 593
CviKI_1 RGCY 6 cut(s) 23, 49, 130, 171, 490, 593
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 1 cut(s) 45
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eco32I GATATC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 626
EcoRV GATATC 1 cut(s) 468
FaeI CATG 5 cut(s) 208, 372, 379, 574, 623
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 668
FatI CATG 5 cut(s) 204, 368, 375, 570, 619
FokI GGATG 3 cut(s) 16, 134, 392
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 5 cut(s) 208, 372, 379, 574, 623
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 272, 631, 636
HphI GGTGA 3 cut(s) 90, 586, 616
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 4 cut(s) 205, 235, 245, 533
Hpy8I GTNNAC 1 cut(s) 98
HpyAV CCTTC 4 cut(s) 27, 459, 571, 661
HpyCH4III ACNGT 3 cut(s) 261, 293, 511
HpyCH4V TGCA 4 cut(s) 125, 316, 325, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 322
HpyF3I CTNAG 1 cut(s) 45
Hsp92II CATG 5 cut(s) 208, 372, 379, 574, 623
Kzo9I GATC 1 cut(s) 355
LweI GCATC 1 cut(s) 112
MaeIII GTNAC 5 cut(s) 161, 176, 371, 622, 670
MalI GATC 1 cut(s) 357
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 355
MboII GAAGA 8 cut(s) 3, 16, 151, 308, 316, 410, 447, 740
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 355
MluCI AATT 5 cut(s) 189, 240, 398, 407, 714
MlyI GAGTC 2 cut(s) 266, 625
MmeI TCCRAC 2 cut(s) 99, 691
MseI TTAA 4 cut(s) 153, 183, 402, 440
MslI CAYNNNNRTG 2 cut(s) 203, 369
MspA1I CMGCKG 2 cut(s) 23, 341
MspR9I CCNGG 1 cut(s) 628
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 628
MwoI GCNNNNNNNGC 1 cut(s) 322
NdeII GATC 1 cut(s) 355
NlaIII CATG 5 cut(s) 208, 372, 379, 574, 623
NlaIV GGNNCC 2 cut(s) 212, 447
NmuCI GTSAC 3 cut(s) 371, 622, 670
NspI RCATGY 1 cut(s) 623
PagI TCATGA 1 cut(s) 204
PciI ACATGT 1 cut(s) 619
PfeI GAWTC 1 cut(s) 636
PleI GAGTC 2 cut(s) 266, 625
PpsI GAGTC 2 cut(s) 266, 625
PscI ACATGT 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 626
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 2 cut(s) 212, 447
PspPI GGNCC 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
RseI CAYNNNNRTG 2 cut(s) 203, 369
SaqAI TTAA 4 cut(s) 153, 183, 402, 440
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 266, 625
ScrFI CCNGG 1 cut(s) 628
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 510
SmiMI CAYNNNNRTG 2 cut(s) 203, 369
SmlI CTYRAG 2 cut(s) 50, 233
SmoI CTYRAG 2 cut(s) 50, 233
Sse9I AATT 5 cut(s) 189, 240, 398, 407, 714
SsiI CCGC 2 cut(s) 40, 341
StyD4I CCNGG 1 cut(s) 626
TaaI ACNGT 3 cut(s) 261, 293, 511
TaqI TCGA 3 cut(s) 244, 634, 702
TasI AATT 5 cut(s) 189, 240, 398, 407, 714
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 4 cut(s) 153, 183, 402, 440
Tru9I TTAA 4 cut(s) 153, 183, 402, 440
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 3 cut(s) 371, 622, 670
Tsp45I GTSAC 3 cut(s) 371, 622, 670
TspDTI ATGAA 7 cut(s) 17, 357, 400, 420, 441, 688, 741
TspRI CASTG 1 cut(s) 514
XapI RAATTY 2 cut(s) 240, 407
XceI RCATGY 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.