RLG00000008075

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008345
Physical Location & Seq
Forward (+)
25339791 .. 25342320
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008075

Sequence Viewer

Length: 732 bp
ATGAAGGCTTTGAGCACATCTCATCTTTTCTCTTCCGTGAAGTCTTTGTCAGACAAAGTAGGAGGCATGCTTTGTTGTCGTAGTTCGGCAAACAAGTATAGCAGGCTGGATGCAAAGCTTGAAAAGAGAATGGCTGAATTTAAAAGAGGTTCTTCAGGCCCAAGCAATTTTAAGTCAATTGACAGCATAATTTTGAGGTTCCCTCAGTTTAGGGAAGAACTGAAGAACATAAGAAATGTGTTTGACCTGTATGATGAGGACTCAAATGGAGCTATAGACCGCGAGGAACTGAAGAAATGCTTACAGAAACTGCAACTCCATCTGACAGCAGAAGAGATTGAGGATCTTTTTCATTCATGTGACATGGATGGGAGTGAAGGGATACAATTCAATGAATTCATTGTTCTTCTATGTCTCATCTATCTATTAAAGGACCCTCACACGACATCGAAGATAGACTCTCCACAGCTAGAAGCAACTTTTGATACTGTCATTGAAGTATTTTTGTTTCTGGATAAAAATGGTGATGGAAAACTGAACAAGACAGAAGTGGTGAAGGCACTGAATGAAGCTTCTCCGCGGGAAAAATCTCCTGCTCATGTCAGCAGGAATCGATTCAAGGAAATGGATTGGGACAAAAGTGGGAAGGTGAGTTTCAGGCAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGAAACAGATGAAGAAATATCTCAAGAACAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

244

Amino Acids

27.84

Weight (kDa)

5.4

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 76 - 134 1.7e-11 EF-hand domain pair
EF-hand_1 PF00036 77 - 104 7.1e-06 EF hand domain
EF-hand_6 PF13405 77 - 104 6.4e-07 EF-hand domain
EF-hand_8 PF13833 88 - 137 6.3e-09 EF-hand domain pair
EF-hand_7 PF13499 164 - 225 5.4e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 282, 580
AciI CCGC 3 cut(s) 280, 578, 580
AclWI GGATC 1 cut(s) 351
AcsI RAATTY 2 cut(s) 137, 395
AcuI CTGAAG 3 cut(s) 138, 242, 311
AgsI TTSAA 5 cut(s) 122, 391, 497, 619, 698
AluBI AGCT 4 cut(s) 118, 272, 469, 572
AluI AGCT 4 cut(s) 118, 272, 469, 572
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 1 cut(s) 351
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 1 cut(s) 157
ApoI RAATTY 2 cut(s) 137, 395
Asp700I GAANNNNTTC 1 cut(s) 614
AspS9I GGNCC 2 cut(s) 158, 433
AsuHPI GGTGA 3 cut(s) 536, 565, 661
AvaII GGWCC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 17
BccI CCATC 3 cut(s) 327, 362, 521
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 1 cut(s) 470
BfmI CTRYAG 1 cut(s) 273
BfuI GTATCC 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 158, 433
BmiI GGNNCC 2 cut(s) 200, 435
BmsI GCATC 1 cut(s) 100
BplI GAGNNNNNCTC 3 cut(s) 36, 187, 219
BpuEI CTTGAG 1 cut(s) 702
Bsa29I ATCGAT 2 cut(s) 613, 681
BsaJI CCNNGG 1 cut(s) 578
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
BseCI ATCGAT 2 cut(s) 613, 681
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 2 cut(s) 115, 373
BseMII CTCAG 1 cut(s) 218
Bsh1236I CGCG 2 cut(s) 282, 580
BshFI GGCC 1 cut(s) 159
BshVI ATCGAT 2 cut(s) 613, 681
BsiHKAI GWGCWC 1 cut(s) 17
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 3 cut(s) 280, 578, 580
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 2 cut(s) 613, 681
BspFNI CGCG 2 cut(s) 282, 580
BspLI GGNNCC 2 cut(s) 200, 435
BspPI GGATC 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 1 cut(s) 343
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 2 cut(s) 37, 327
BstC8I GCNNGC 2 cut(s) 68, 104
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 2 cut(s) 115, 373
BstFNI CGCG 2 cut(s) 282, 580
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 273
BstUI CGCG 2 cut(s) 282, 580
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
Bsu15I ATCGAT 2 cut(s) 613, 681
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 159
BsuTUI ATCGAT 2 cut(s) 613, 681
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 115, 373
BtsIMutI CAGTG 1 cut(s) 560
Cac8I GCNNGC 2 cut(s) 68, 104
CaiI CAGNNNCTG 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 158, 433
Cfr42I CCGCGG 1 cut(s) 581
ClaI ATCGAT 2 cut(s) 613, 681
CviAII CATG 4 cut(s) 67, 357, 364, 599
CviJI RGCY 8 cut(s) 8, 106, 118, 134, 159, 272, 469, 572
CviKI_1 RGCY 8 cut(s) 8, 106, 118, 134, 159, 272, 469, 572
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
DraI TTTAAA 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 37, 327
EarI CTCTTC 2 cut(s) 37, 327
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 3 cut(s) 138, 242, 311
EcoO109I RGGNCCY 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 395
FaeI CATG 4 cut(s) 70, 360, 367, 602
FalI AAGNNNNNCTT 4 cut(s) 136, 168, 284, 316
FaqI GGGAC 1 cut(s) 647
FatI CATG 4 cut(s) 66, 356, 363, 598
FauI CCCGC 1 cut(s) 573
FokI GGATG 2 cut(s) 122, 380
FspBI CTAG 1 cut(s) 470
HaeIII GGCC 1 cut(s) 159
Hin1II CATG 4 cut(s) 70, 360, 367, 602
HindIII AAGCTT 2 cut(s) 116, 570
HinfI GANTC 4 cut(s) 260, 458, 610, 615
HphI GGTGA 3 cut(s) 536, 565, 661
Hpy188I TCNGA 2 cut(s) 52, 324
Hpy188III TCNNGA 2 cut(s) 512, 719
HpyAV CCTTC 3 cut(s) 371, 550, 640
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 2 cut(s) 113, 313
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 4 cut(s) 70, 360, 367, 602
KspI CCGCGG 1 cut(s) 581
Kzo9I GATC 1 cut(s) 343
LmnI GCTCC 1 cut(s) 269
LpnPI CCDG 8 cut(s) 88, 92, 141, 260, 497, 592, 606, 643
LweI GCATC 1 cut(s) 100
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 1 cut(s) 359
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 9 cut(s) 24, 144, 227, 235, 304, 344, 398, 463, 719
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 8 cut(s) 137, 166, 177, 189, 386, 395, 693, 727
MlyI GAGTC 2 cut(s) 254, 452
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 9 cut(s) 56, 140, 189, 213, 250, 277, 334, 447, 677
MroXI GAANNNNTTC 1 cut(s) 614
MseI TTAA 4 cut(s) 141, 171, 428, 730
MslI CAYNNNNRTG 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 580
MunI CAATTG 1 cut(s) 177
MvnI CGCG 2 cut(s) 282, 580
NdeII GATC 1 cut(s) 343
NlaIII CATG 4 cut(s) 70, 360, 367, 602
NlaIV GGNNCC 2 cut(s) 200, 435
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 2 cut(s) 610, 615
PleI GAGTC 2 cut(s) 254, 452
PpsI GAGTC 2 cut(s) 254, 452
PpuMI RGGWCCY 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 200, 435
PspPI GGNCC 2 cut(s) 158, 433
PspPPI RGGWCCY 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 357
SacII CCGCGG 1 cut(s) 581
SaqAI TTAA 4 cut(s) 141, 171, 428, 730
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 2 cut(s) 158, 433
SchI GAGTC 2 cut(s) 254, 452
SduI GDGCHC 1 cut(s) 17
SetI ASST 8 cut(s) 120, 151, 200, 249, 274, 471, 574, 651
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 1 cut(s) 273
Sfr303I CCGCGG 1 cut(s) 581
SgrBI CCGCGG 1 cut(s) 581
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
SphI GCATGC 1 cut(s) 70
Sse9I AATT 8 cut(s) 137, 166, 177, 189, 386, 395, 693, 727
SsiI CCGC 3 cut(s) 280, 578, 580
SspMI CTAG 1 cut(s) 470
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 449, 613, 681
TasI AATT 8 cut(s) 137, 166, 177, 189, 386, 395, 693, 727
TfiI GAWTC 2 cut(s) 610, 615
Tru1I TTAA 4 cut(s) 141, 171, 428, 730
Tru9I TTAA 4 cut(s) 141, 171, 428, 730
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 359
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 8 cut(s) 17, 341, 345, 388, 408, 582, 667, 720
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 2 cut(s) 137, 395
XceI RCATGY 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.