Rorug04G0133600

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008345
Physical Location & Seq
Forward (+)
21510838 .. 21514186
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0133600.1

Sequence Viewer

Length: 726 bp
ATGCTTATAGAAGAAGGAGAAGAAGAGCACAATGTGAAGACCCCAAAACCCTCGTCGTCCTCCGCCAACAAAGACGCCTCCGATGGCTTCGAAACTGCCAGCGACGGCGAGTTGGGACCCAACGAAAGCGACGATGAAACTCAACGCCACGTCCAACTTGAAGAGACGGATGACCTTGCATTCAAACAGAAATCGCTTGAGCAAGCAAATGAGGTAAAATTGGAAGGCAATGGACTGTTTGGGAATGGACAATATGAAGAGGCATTGTCGCAGTATGAACTTGCTTTACAGCTTGCGCCGGACATGCCTTCATCCGTGGAATTGCGTTCCATATGTCATTCGAACCGTGCCATATGCTTTTCGAAGCTGGGAAAATACGAAGACGCGGTTAAGGAATGCACAAAAGCACTGGAACTAAATCCTTCATATATGAAAGCGCTGATTAGAAGAGCAGAAGCTCATGAAAAACTAGAACATTTCGAAGAGGCTATTGCTGATCTGAAAAAAATCTTAGAACTTGATCCTTCAAATGACCAAGCCAGGAAGGCCATTCGGCGCTTGGAGCCACTTGCTGAAGCAAAGAGAGAAAAGATGAAAGAGGAGATGATTGGGAAGTTGAAAGATATGGGCAACTCGTTGTTGGGCCGGTTTGGAATGAGCGTTGACAACTTCAAAGCTGTAAAAGATCCAAACACTGGCTCATATTCTGTTTCGTTCCAACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

241

Amino Acids

26.97

Weight (kDa)

4.95

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_1 PF00515 112 - 143 4.5e-09 Tetratricopeptide repeat
TPR_2 PF07719 112 - 142 2.4e-06 Tetratricopeptide repeat
TPR_16 PF13432 122 - 177 1.5e-06 Tetratricopeptide repeat
TPR_19 PF14559 124 - 186 2.6e-07 Tetratricopeptide repeat
TPR_1 PF00515 145 - 177 4.7e-07 Tetratricopeptide repeat
TPR_2 PF07719 145 - 177 1.8e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 695
AccII CGCG 1 cut(s) 386
AciI CCGC 2 cut(s) 63, 386
AclI AACGTT 1 cut(s) 721
AclWI GGATC 2 cut(s) 515, 680
AcuI CTGAAG 1 cut(s) 594
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 34
AfeI AGCGCT 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 695
AgsI TTSAA 5 cut(s) 161, 184, 528, 619, 673
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 4 cut(s) 292, 367, 458, 677
AluI AGCT 4 cut(s) 292, 367, 458, 677
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 2 cut(s) 515, 680
Aor51HI AGCGCT 1 cut(s) 438
AoxI GGCC 2 cut(s) 546, 643
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AspLEI GCGC 3 cut(s) 298, 439, 558
AspS9I GGNCC 2 cut(s) 116, 643
AsuII TTCGAA 4 cut(s) 90, 341, 362, 480
AvaII GGWCC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 44, 387
Bbv12I GWGCWC 1 cut(s) 30
BccI CCATC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 1 cut(s) 470
BfoI RGCGCY 2 cut(s) 440, 559
BglI GCCNNNNNGGC 1 cut(s) 545
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 116, 643
BmiI GGNNCC 3 cut(s) 117, 118, 564
BmrFI CCNGG 1 cut(s) 541
BpiI GAAGAC 2 cut(s) 44, 387
Bpu14I TTCGAA 4 cut(s) 90, 341, 362, 480
BpuEI CTTGAG 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse118I RCCGGY 1 cut(s) 645
Bse1I ACTGG 2 cut(s) 414, 700
Bse3DI GCAATG 1 cut(s) 235
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 175, 311
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 1 cut(s) 235
BseNI ACTGG 2 cut(s) 414, 700
BseRI GAGGAG 1 cut(s) 614
BseYI CCCAGC 1 cut(s) 367
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 2 cut(s) 548, 645
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 299, 646
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 1 cut(s) 158
BsmBI CGTCTC 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 129
BsmI GAATGC 2 cut(s) 179, 401
BsnI GGCC 2 cut(s) 548, 645
Bsp119I TTCGAA 4 cut(s) 90, 341, 362, 480
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 3 cut(s) 496, 520, 685
BspACI CCGC 2 cut(s) 63, 386
BspANI GGCC 2 cut(s) 548, 645
BspFNI CGCG 1 cut(s) 386
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 117, 118, 564
BspPI GGATC 2 cut(s) 515, 680
BspQI GCTCTTC 2 cut(s) 18, 442
BspT104I TTCGAA 4 cut(s) 90, 341, 362, 480
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 645
BsrI ACTGG 2 cut(s) 414, 700
BssAI RCCGGY 1 cut(s) 645
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 496, 520, 685
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 2 cut(s) 237, 347
Bst6I CTCTTC 5 cut(s) 18, 156, 252, 442, 477
BstACI GRCGYC 1 cut(s) 75
BstBI TTCGAA 4 cut(s) 90, 341, 362, 480
BstC8I GCNNGC 3 cut(s) 100, 204, 294
BstDEI CTNAG 1 cut(s) 511
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 175, 311
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 2 cut(s) 440, 559
BstHHI GCGC 3 cut(s) 298, 439, 558
BstKTI GATC 3 cut(s) 499, 523, 688
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 3 cut(s) 496, 520, 685
BstMWI GCNNNNNNNGC 3 cut(s) 304, 545, 562
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 539
BstUI CGCG 1 cut(s) 386
BstV2I GAAGAC 2 cut(s) 44, 387
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 2 cut(s) 548, 645
BtgI CCRYGG 1 cut(s) 315
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 2 cut(s) 175, 311
BtsIMutI CAGTG 2 cut(s) 407, 693
Cac8I GCNNGC 3 cut(s) 100, 204, 294
CciI TCATGA 1 cut(s) 460
CfoI GCGC 3 cut(s) 298, 439, 558
Cfr10I RCCGGY 1 cut(s) 645
Cfr13I GGNCC 2 cut(s) 116, 643
CseI GACGC 2 cut(s) 83, 392
CviAII CATG 2 cut(s) 304, 461
DdeI CTNAG 1 cut(s) 511
DpnI GATC 3 cut(s) 498, 522, 687
DpnII GATC 3 cut(s) 496, 520, 685
DraIII CACNNNGTG 1 cut(s) 34
Eam1104I CTCTTC 5 cut(s) 18, 156, 252, 442, 477
EarI CTCTTC 5 cut(s) 18, 156, 252, 442, 477
EciI GGCGGA 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 116
Eco47III AGCGCT 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 539
Esp3I CGTCTC 1 cut(s) 158
FaeI CATG 2 cut(s) 307, 464
FaqI GGGAC 1 cut(s) 129
FatI CATG 2 cut(s) 303, 460
FauNDI CATATG 2 cut(s) 332, 353
FokI GGATG 2 cut(s) 182, 298
FspBI CTAG 1 cut(s) 470
GlaI GCGC 3 cut(s) 297, 438, 557
GsaI CCCAGC 1 cut(s) 371
HaeII RGCGCY 2 cut(s) 440, 559
HaeIII GGCC 2 cut(s) 548, 645
HapII CCGG 2 cut(s) 299, 646
HgaI GACGC 2 cut(s) 83, 392
HhaI GCGC 3 cut(s) 298, 439, 558
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 307, 464
Hin6I GCGC 3 cut(s) 296, 437, 556
HinP1I GCGC 3 cut(s) 296, 437, 556
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HpaII CCGG 2 cut(s) 299, 646
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 2 cut(s) 82, 501
Hpy188III TCNNGA 1 cut(s) 461
Hpy8I GTNNAC 1 cut(s) 664
Hpy99I CGWCG 3 cut(s) 58, 107, 134
HpyAV CCTTC 6 cut(s) 8, 218, 318, 432, 534, 538
HpyCH4III ACNGT 2 cut(s) 237, 347
HpyCH4IV ACGT 2 cut(s) 150, 721
HpyCH4V TGCA 2 cut(s) 179, 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 545, 562
HpyF3I CTNAG 1 cut(s) 511
HpySE526I ACGT 2 cut(s) 150, 721
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 307, 464
HspAI GCGC 3 cut(s) 296, 437, 556
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 3 cut(s) 496, 520, 685
LguI GCTCTTC 2 cut(s) 18, 442
LmnI GCTCC 1 cut(s) 562
LpnPI CCDG 8 cut(s) 112, 312, 353, 395, 526, 553, 659, 681
MaeI CTAG 1 cut(s) 470
MaeII ACGT 2 cut(s) 150, 721
MalI GATC 3 cut(s) 498, 522, 687
MboI GATC 3 cut(s) 496, 520, 685
MboII GAAGA 9 cut(s) 23, 32, 35, 49, 173, 269, 392, 459, 494
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 218, 320
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 7 cut(s) 61, 70, 88, 205, 253, 478, 592
MseI TTAA 1 cut(s) 390
MspI CCGG 2 cut(s) 299, 646
MspR9I CCNGG 1 cut(s) 541
Mva1269I GAATGC 2 cut(s) 179, 401
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 3 cut(s) 304, 545, 562
NdeI CATATG 2 cut(s) 332, 353
NdeII GATC 3 cut(s) 496, 520, 685
NlaIII CATG 2 cut(s) 307, 464
NlaIV GGNNCC 3 cut(s) 117, 118, 564
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 4 cut(s) 90, 341, 362, 480
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 18, 442
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 179, 401
PflMI CCANNNNNTGG 1 cut(s) 695
PpuMI RGGWCCY 1 cut(s) 116
Psp1406I AACGTT 1 cut(s) 721
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 3 cut(s) 117, 118, 564
PspPI GGNCC 2 cut(s) 116, 643
PspPPI RGGWCCY 1 cut(s) 116
PsrI GAACNNNNNNTAC 2 cut(s) 270, 302
PsuI RGATCY 1 cut(s) 685
SapI GCTCTTC 2 cut(s) 18, 442
SaqAI TTAA 1 cut(s) 390
Sau3AI GATC 3 cut(s) 496, 520, 685
Sau96I GGNCC 2 cut(s) 116, 643
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 1 cut(s) 30
SetI ASST 8 cut(s) 153, 177, 216, 294, 369, 460, 679, 724
SfuI TTCGAA 4 cut(s) 90, 341, 362, 480
SinI GGWCC 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 218, 320
SsiI CCGC 2 cut(s) 63, 386
SspMI CTAG 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 539
TaaI ACNGT 2 cut(s) 237, 347
TaiI ACGT 2 cut(s) 153, 724
TaqI TCGA 4 cut(s) 90, 341, 362, 480
TasI AATT 2 cut(s) 218, 320
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 414, 700
TspDTI ATGAA 8 cut(s) 150, 270, 291, 300, 414, 446, 477, 608
TspGWI ACGGA 2 cut(s) 182, 304
TspRI CASTG 2 cut(s) 414, 700
Van91I CCANNNNNTGG 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 116
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.