Prupe.1G007100_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008345
Physical Location & Seq
Forward (+)
594535 .. 597085
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G007100.1

Sequence Viewer

Length: 756 bp
ATGAAGGGTTCAACTTCAACAGGCACATCTCATTGCCCTTATCTGAAGTCATTATCAAACAAAGTAGGAAGCATGCTTTGTTGTCGTGGTTCAACCAACAAGTATAGCAGGTTGGATGCAAAGCTTGAAAAGAAAATGGTTGAATTTAAAAGAGGTTCCTTGGGCCGTAGCAATTTCAAGTCAATTGACAGCATAATCATGAGGTTCCCTCAGTTCAGGGAGGAACTCAAGAAAATAAGAGATGTCTTTGAACAGTATGATGAGGACTCAAATGGAACTATTGACCTTGAGGAACTGAAGAAATGCTTACAGAAACTGGAACTCCATCTGACAGCGGAGGAGATTGAGGATCTTTTTCATTCATGTGACATGGATGGGAGTGAAGGGATACAATTCAATGAATTCATTGTTCTTCTATGCCTCATCTATCTATTAAAGGAACCTTCATCCTCTCATAATACATCCAAGATAGATTCGCCACAGCTTGAAGCAACTTTTGACACCGTAATTGAAGTATTCTCATTTCTGGATAAAAATGGTGATGGGAAACTGAACAAGACAGACATGGTGAAGGCATTGAATGAGGCCTCTCCTTTGGAGAAATCTCCTGCACATGTCACCAGGACTCGATTCAAAGAAATGGATTGGAGCAAACGTGGGAAGGTCAGTTTCAGGGAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGACACAAATGAAGAAATGTCTCCAGTACGAAGTTCAAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

252

Amino Acids

28.69

Weight (kDa)

6.03

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 99
AciI CCGC 1 cut(s) 335
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 2 cut(s) 143, 401
AcuI CTGAAG 2 cut(s) 65, 317
AfaI GTAC 1 cut(s) 738
AflIII ACRYGT 1 cut(s) 613
AjnI CCWGG 1 cut(s) 620
AluBI AGCT 2 cut(s) 124, 484
AluI AGCT 2 cut(s) 124, 484
Alw26I GTCTC 1 cut(s) 735
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 2 cut(s) 163, 585
ApoI RAATTY 2 cut(s) 143, 401
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 551, 580, 610
BccI CCATC 3 cut(s) 333, 368, 536
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 622
BciVI GTATCC 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 735
BfuAI ACCTGC 1 cut(s) 99
BfuI GTATCC 1 cut(s) 381
Bme1390I CCNGG 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 3 cut(s) 157, 206, 441
BmrFI CCNGG 1 cut(s) 622
BmsI GCATC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 193, 225
BpmI CTGGAG 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 212, 308
Bsa29I ATCGAT 1 cut(s) 696
BsaJI CCNNGG 1 cut(s) 159
BsaXI ACNNNNNCTCC 4 cut(s) 306, 336, 640, 670
Bse1I ACTGG 2 cut(s) 321, 734
Bse3DI GCAATG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 622
BseCI ATCGAT 1 cut(s) 696
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 4 cut(s) 121, 379, 446, 461
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 2 cut(s) 321, 734
BseRI GAGGAG 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 2 cut(s) 165, 587
BshVI ATCGAT 1 cut(s) 696
BsmAI GTCTC 1 cut(s) 735
BsnI GGCC 2 cut(s) 165, 587
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 1 cut(s) 335
BspANI GGCC 2 cut(s) 165, 587
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 696
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 157, 206, 441
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 2 cut(s) 321, 734
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 2 cut(s) 255, 505
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 121, 379, 446, 461
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 1 cut(s) 735
BstMBI GATC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 622
BstNSI RCATGY 2 cut(s) 76, 617
BstSCI CCNGG 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 696
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 2 cut(s) 165, 587
BsuTUI ATCGAT 1 cut(s) 696
BtsCI GGATG 4 cut(s) 121, 379, 446, 461
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 74
CaiI CAGNNNCTG 1 cut(s) 316
CciI TCATGA 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 696
Csp6I GTAC 1 cut(s) 737
CviAII CATG 6 cut(s) 73, 199, 363, 370, 565, 614
CviJI RGCY 4 cut(s) 124, 165, 484, 587
CviKI_1 RGCY 4 cut(s) 124, 165, 484, 587
CviQI GTAC 1 cut(s) 737
DdeI CTNAG 1 cut(s) 210
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DraI TTTAAA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 65, 317
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 6 cut(s) 76, 202, 366, 373, 568, 617
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 6 cut(s) 72, 198, 362, 369, 564, 613
FokI GGATG 4 cut(s) 128, 386, 433, 448
GsuI CTGGAG 1 cut(s) 717
HaeIII GGCC 2 cut(s) 165, 587
Hin1II CATG 6 cut(s) 76, 202, 366, 373, 568, 617
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 4 cut(s) 266, 473, 625, 630
HphI GGTGA 3 cut(s) 551, 580, 610
Hpy188I TCNGA 2 cut(s) 45, 330
Hpy188III TCNNGA 3 cut(s) 199, 229, 527
HpyAV CCTTC 4 cut(s) 377, 453, 565, 655
HpyCH4III ACNGT 2 cut(s) 255, 505
HpyCH4IV ACGT 1 cut(s) 655
HpyCH4V TGCA 2 cut(s) 119, 611
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 655
Hsp92II CATG 6 cut(s) 76, 202, 366, 373, 568, 617
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 648
LweI GCATC 1 cut(s) 106
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 2 cut(s) 365, 616
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 3 cut(s) 310, 404, 734
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 349
MluCI AATT 7 cut(s) 143, 172, 183, 392, 401, 507, 708
MlyI GAGTC 2 cut(s) 260, 619
MmeI TCCRAC 2 cut(s) 93, 685
MseI TTAA 2 cut(s) 147, 434
MslI CAYNNNNRTG 2 cut(s) 197, 363
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 622
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 622
NdeII GATC 1 cut(s) 349
NlaIII CATG 6 cut(s) 76, 202, 366, 373, 568, 617
NlaIV GGNNCC 3 cut(s) 157, 206, 441
NmuCI GTSAC 2 cut(s) 365, 616
NspI RCATGY 2 cut(s) 76, 617
PaeI GCATGC 1 cut(s) 76
PagI TCATGA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 587
PciI ACATGT 1 cut(s) 613
PfeI GAWTC 2 cut(s) 473, 630
PleI GAGTC 2 cut(s) 260, 619
PpsI GAGTC 2 cut(s) 260, 619
PscI ACATGT 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 3 cut(s) 157, 206, 441
PspPI GGNCC 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 738
RsaNI GTAC 1 cut(s) 737
RseI CAYNNNNRTG 2 cut(s) 197, 363
SaqAI TTAA 2 cut(s) 147, 434
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 2 cut(s) 260, 619
ScrFI CCNGG 1 cut(s) 622
SetI ASST 9 cut(s) 113, 126, 157, 206, 288, 445, 486, 658, 666
SfaNI GCATC 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 197, 363
SmlI CTYRAG 2 cut(s) 227, 287
SmoI CTYRAG 2 cut(s) 227, 287
SphI GCATGC 1 cut(s) 76
Sse9I AATT 7 cut(s) 143, 172, 183, 392, 401, 507, 708
SseBI AGGCCT 1 cut(s) 587
SsiI CCGC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 620
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 255, 505
TaiI ACGT 1 cut(s) 658
TaqI TCGA 2 cut(s) 628, 696
TasI AATT 7 cut(s) 143, 172, 183, 392, 401, 507, 708
TfiI GAWTC 2 cut(s) 473, 630
Tru1I TTAA 2 cut(s) 147, 434
Tru9I TTAA 2 cut(s) 147, 434
TseFI GTSAC 2 cut(s) 365, 616
Tsp45I GTSAC 2 cut(s) 365, 616
TspDTI ATGAA 8 cut(s) 17, 347, 351, 394, 414, 435, 682, 735
XapI RAATTY 2 cut(s) 143, 401
XceI RCATGY 2 cut(s) 76, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.