Rh4DG192000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008345
Physical Location & Seq
Reverse (-)
37719401 .. 37726320
6920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG192000.1

Sequence Viewer

Length: 777 bp
ATGCTTTGTTGTCGTGGTTCGGCAAACAAGTATAGCAGGCTGGATGCAAAGCTTGAAAAGAGAATGGCTGAATTTAAAAGAGGTTCTTCAGGCCAAAGCAATTTTAAGTCAATTGACAGCATAATTATGAGGTTCCCTCAGTTTAGGGAAGAACTGAAGAACATAAGAAATGTGTTTGACCTGTATGATGAGGACTCAAATGGAGCTATAGACCGCGAGGAACTGAAGAAATGCTTACAGAAACTGCAACTCCATCTGACAGCAGAAGAGATTGAGGATCTTTTTCATTCATGTGACATGGATGGGAGTGAAGGGATACAATTCAATGAATTCATTGTTCTTCTATGTCTCATCTATCTATTAAAGGACCCTCAAACGACATCGAAGATAGACTCTCCACAGCTAGAAGCAACTTTTGATACTGTCATTGAAGTATTTTTGTTTCTGGATAAAAATGGTGATGGAAAACTGAACAAGACAGAAGTGGTGAAGGCACTGAATGAAGCTTCTCCGCGGGAGAAATCTCCTGCTCATGTCAGCAGGAATCGATTCAGTATGATTCTTTGCTTCTACAGTAAAATTGTCATTTCTCCTTTCTTTTACATGGATACGCAGTTCTCATCATTAGTTTTTTTTTTTTTTTTTGCTTTGTGGGTTGTTGCAGAGGAAATGGATTGGGACAAAAGTGGGAAGGTGAGTTTCAGGCAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGAAACAGATGAAGGAATATCTCAAGAACAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

258

Amino Acids

29.91

Weight (kDa)

4.96

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 54 - 112 1.8e-11 EF-hand domain pair
EF-hand_6 PF13405 55 - 82 7e-07 EF-hand domain
EF-hand_1 PF00036 55 - 82 7.7e-06 EF hand domain
EF-hand_8 PF13833 66 - 115 7.8e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 216, 514
AciI CCGC 3 cut(s) 214, 512, 514
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 2 cut(s) 71, 329
AcuI CTGAAG 3 cut(s) 72, 176, 245
AgsI TTSAA 4 cut(s) 56, 325, 431, 743
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 4 cut(s) 52, 206, 403, 506
AluI AGCT 4 cut(s) 52, 206, 403, 506
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 1 cut(s) 285
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 1 cut(s) 91
ApoI RAATTY 2 cut(s) 71, 329
Asp700I GAANNNNTTC 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 3 cut(s) 470, 499, 706
AvaII GGWCC 1 cut(s) 367
BccI CCATC 3 cut(s) 261, 296, 455
BciVI GTATCC 2 cut(s) 309, 601
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 2 cut(s) 207, 571
BfuI GTATCC 2 cut(s) 309, 601
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 2 cut(s) 134, 369
BmsI GCATC 1 cut(s) 34
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BpuEI CTTGAG 1 cut(s) 747
Bsa29I ATCGAT 2 cut(s) 547, 726
BsaJI CCNNGG 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
BseCI ATCGAT 2 cut(s) 547, 726
BseDI CCNNGG 1 cut(s) 512
BseGI GGATG 2 cut(s) 49, 307
BseMII CTCAG 1 cut(s) 152
Bsh1236I CGCG 2 cut(s) 216, 514
BshFI GGCC 1 cut(s) 93
BshVI ATCGAT 2 cut(s) 547, 726
BslFI GGGAC 1 cut(s) 692
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 1 cut(s) 93
Bsp143I GATC 1 cut(s) 277
BspACI CCGC 3 cut(s) 214, 512, 514
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 151
BspDI ATCGAT 2 cut(s) 547, 726
BspFNI CGCG 2 cut(s) 216, 514
BspLI GGNNCC 2 cut(s) 134, 369
BspPI GGATC 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 512
BssMI GATC 1 cut(s) 277
Bst4CI ACNGT 2 cut(s) 424, 575
Bst6I CTCTTC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 512
BstF5I GGATG 2 cut(s) 49, 307
BstFNI CGCG 2 cut(s) 216, 514
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 1 cut(s) 277
BstSFI CTRYAG 2 cut(s) 207, 571
BstUI CGCG 2 cut(s) 216, 514
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 2 cut(s) 547, 726
BsuI GTATCC 2 cut(s) 309, 601
BsuRI GGCC 1 cut(s) 93
BsuTUI ATCGAT 2 cut(s) 547, 726
BtgI CCRYGG 1 cut(s) 512
BtsCI GGATG 2 cut(s) 49, 307
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 367
Cfr42I CCGCGG 1 cut(s) 515
ClaI ATCGAT 2 cut(s) 547, 726
CviAII CATG 4 cut(s) 291, 298, 533, 604
CviJI RGCY 7 cut(s) 40, 52, 68, 93, 206, 403, 506
CviKI_1 RGCY 7 cut(s) 40, 52, 68, 93, 206, 403, 506
DdeI CTNAG 1 cut(s) 138
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 367
Eco57I CTGAAG 3 cut(s) 72, 176, 245
EcoO109I RGGNCCY 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 329
FaeI CATG 4 cut(s) 294, 301, 536, 607
FalI AAGNNNNNCTT 4 cut(s) 70, 102, 218, 250
FaqI GGGAC 1 cut(s) 692
FatI CATG 4 cut(s) 290, 297, 532, 603
FauI CCCGC 1 cut(s) 507
FokI GGATG 2 cut(s) 56, 314
FspBI CTAG 1 cut(s) 404
HaeIII GGCC 1 cut(s) 93
Hin1II CATG 4 cut(s) 294, 301, 536, 607
HindIII AAGCTT 2 cut(s) 50, 504
HinfI GANTC 5 cut(s) 194, 392, 544, 549, 559
HphI GGTGA 3 cut(s) 470, 499, 706
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 446, 764
HpyAV CCTTC 4 cut(s) 305, 484, 685, 746
HpyCH4III ACNGT 2 cut(s) 424, 575
HpyCH4V TGCA 3 cut(s) 47, 247, 662
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 4 cut(s) 294, 301, 536, 607
KspI CCGCGG 1 cut(s) 515
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 8 cut(s) 22, 26, 75, 194, 431, 526, 540, 688
LweI GCATC 1 cut(s) 34
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 7 cut(s) 78, 161, 169, 238, 278, 332, 397
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 277
MluCI AATT 8 cut(s) 71, 100, 111, 123, 320, 329, 579, 738
MlyI GAGTC 2 cut(s) 188, 386
MmeI TCCRAC 1 cut(s) 715
MnlI CCTC 9 cut(s) 74, 123, 147, 184, 211, 268, 381, 658, 722
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 4 cut(s) 75, 105, 362, 775
MslI CAYNNNNRTG 2 cut(s) 125, 291
MspA1I CMGCKG 1 cut(s) 514
MunI CAATTG 1 cut(s) 111
MvnI CGCG 2 cut(s) 216, 514
NdeII GATC 1 cut(s) 277
NlaIII CATG 4 cut(s) 294, 301, 536, 607
NlaIV GGNNCC 2 cut(s) 134, 369
NmuCI GTSAC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 3 cut(s) 544, 549, 559
PleI GAGTC 2 cut(s) 188, 386
PpsI GAGTC 2 cut(s) 188, 386
PpuMI RGGWCCY 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 367
PspN4I GGNNCC 2 cut(s) 134, 369
PspPI GGNCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 277
RseI CAYNNNNRTG 2 cut(s) 125, 291
SacII CCGCGG 1 cut(s) 515
SaqAI TTAA 4 cut(s) 75, 105, 362, 775
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 2 cut(s) 188, 386
SetI ASST 8 cut(s) 54, 85, 134, 183, 208, 405, 508, 696
SfaNI GCATC 1 cut(s) 34
SfcI CTRYAG 2 cut(s) 207, 571
Sfr303I CCGCGG 1 cut(s) 515
SgrBI CCGCGG 1 cut(s) 515
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 125, 291
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
Sse9I AATT 8 cut(s) 71, 100, 111, 123, 320, 329, 579, 738
SsiI CCGC 3 cut(s) 214, 512, 514
SspMI CTAG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 424, 575
TaqI TCGA 3 cut(s) 383, 547, 726
TasI AATT 8 cut(s) 71, 100, 111, 123, 320, 329, 579, 738
TfiI GAWTC 3 cut(s) 544, 549, 559
Tru1I TTAA 4 cut(s) 75, 105, 362, 775
Tru9I TTAA 4 cut(s) 75, 105, 362, 775
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 7 cut(s) 275, 279, 322, 342, 516, 712, 765
TspRI CASTG 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 71, 329
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.