MD04G1008500.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008345
Physical Location & Seq
Forward (+)
978810 .. 981848
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1008500.v1.1.491

Sequence Viewer

Length: 762 bp
ATGAAGACTTCTTCTCCTTCAGCTGGCACATCCCATCTCCGCTCTCAGCTCAAGTCTATGTCAAACAAAGTAGGAGGTATGCTGTGTTGTTGTAGTTCACCAAATAAGTATAGCAGGTTGGATGCGAAGCTTGAAAAGAAGATGGTTGAAGTTAAAAGAGGTTCCTTGGGCCGTAGTAATTTTAAATCAATTGACAGCATTATCATGAGGTTCCCCCAGTTCAGGGAGGAACTCAAGAAAATTCGAGATGTGTTTGAACAGTATGATGAGGACTCAAATGGAACTATTGACCGCGAAGAACTGAAGAAATGCTTGCAGAAACTGCAACTCCATCTGACAGCGGAGGAGATTGAGGATCTTTTCCATTCATGTGACATGGATGGAAGTGAGGGGATACAATTTAACGAATTCATTGTTCTTCTATGCCTCATCTATCTATTAAAGGAACCTTCATCTTCTCATACGATATCAAAGATAAACTCGCCACAGCTTGAAGCAACTTTTGACACTGTAGTTGAAGTATTCTCATTTCTGGATAAAAATGGCGATGGGAAACTGAACAAGACAGACGTGGTGAAGGCATTGAACGAGGCTTCTCCTTTGGAGAAATCCCCTGCACGTGTCACCAGGACTCGATTCAAAGAAATGGATTGGGACAAAAATGGGAAGGTCACTTTCAGGGAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGACACAGACGGAGAAATGTCTCCAGTACGAAGTTCAAGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

254

Amino Acids

28.76

Weight (kDa)

6.47

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 79 - 138 8.7e-12 EF-hand domain pair
EF-hand_6 PF13405 80 - 108 1.2e-07 EF-hand domain
EF-hand_1 PF00036 81 - 108 1.3e-06 EF hand domain
EF-hand_5 PF13202 81 - 105 3e-06 EF hand
EF-hand_8 PF13833 92 - 141 3.9e-09 EF-hand domain pair
EF-hand_7 PF13499 173 - 233 1.7e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 105
AccBSI CCGCTC 1 cut(s) 42
AccII CGCG 1 cut(s) 294
AciI CCGC 3 cut(s) 40, 292, 341
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 2 cut(s) 240, 407
AcuI CTGAAG 1 cut(s) 323
AcvI CACGTG 1 cut(s) 620
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 3 cut(s) 23, 222, 223
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 8 cut(s) 134, 149, 257, 494, 518, 586, 640, 753
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 1 cut(s) 626
AluBI AGCT 4 cut(s) 23, 49, 130, 490
AluI AGCT 4 cut(s) 23, 49, 130, 490
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 1 cut(s) 169
ApoI RAATTY 2 cut(s) 240, 407
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 90, 586, 616
BbrPI CACGTG 1 cut(s) 620
BbsI GAAGAC 1 cut(s) 11
BccI CCATC 5 cut(s) 42, 136, 339, 374, 542
BceAI ACGGC 1 cut(s) 156
BciT130I CCWGG 1 cut(s) 628
BciVI GTATCC 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 387
Bme1390I CCNGG 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 3 cut(s) 163, 212, 447
BmrFI CCNGG 1 cut(s) 628
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 723
BpuEI CTTGAG 2 cut(s) 35, 218
Bsa29I ATCGAT 1 cut(s) 702
BsaAI YACGTR 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 3 cut(s) 28, 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 222, 223
Bse1I ACTGG 2 cut(s) 217, 740
BseBI CCWGG 1 cut(s) 628
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 3 cut(s) 29, 127, 385
BseLI CCNNNNNNNGG 3 cut(s) 23, 222, 223
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 2 cut(s) 217, 740
BseRI GAGGAG 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 702
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 3 cut(s) 23, 222, 223
BsmAI GTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 1 cut(s) 355
BspACI CCGC 3 cut(s) 40, 292, 341
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 1 cut(s) 58
BspDI ATCGAT 1 cut(s) 702
BspFNI CGCG 1 cut(s) 294
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 163, 212, 447
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 1 cut(s) 363
BsrBI CCGCTC 1 cut(s) 42
BsrI ACTGG 2 cut(s) 217, 740
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 261, 511
BstAPI GCANNNNNTGC 1 cut(s) 322
BstBAI YACGTR 1 cut(s) 620
BstC8I GCNNGC 2 cut(s) 25, 314
BstDEI CTNAG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 29, 127, 385
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 322
BstNI CCWGG 1 cut(s) 628
BstSCI CCNGG 1 cut(s) 626
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
Bsu15I ATCGAT 1 cut(s) 702
BsuI GTATCC 1 cut(s) 387
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 3 cut(s) 29, 127, 385
BtsIMutI CAGTG 1 cut(s) 507
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 25, 314
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 702
Csp6I GTAC 1 cut(s) 743
CviAII CATG 3 cut(s) 205, 369, 376
CviJI RGCY 6 cut(s) 23, 49, 130, 171, 490, 593
CviKI_1 RGCY 6 cut(s) 23, 49, 130, 171, 490, 593
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 1 cut(s) 45
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
DraI TTTAAA 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 165
Eco32I GATATC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 323
Eco72I CACGTG 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 626
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 3 cut(s) 208, 372, 379
FaiI YATR 9 cut(s) 59, 80, 111, 206, 264, 370, 377, 424, 462
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 204, 368, 375
FokI GGATG 3 cut(s) 16, 134, 392
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 3 cut(s) 208, 372, 379
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 272, 631, 636
HphI GGTGA 3 cut(s) 90, 586, 616
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 4 cut(s) 205, 235, 245, 533
Hpy8I GTNNAC 1 cut(s) 98
HpyAV CCTTC 4 cut(s) 27, 459, 571, 661
HpyCH4III ACNGT 2 cut(s) 261, 511
HpyCH4IV ACGT 2 cut(s) 570, 619
HpyCH4V TGCA 3 cut(s) 316, 325, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 322
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 2 cut(s) 570, 619
Hsp92II CATG 3 cut(s) 208, 372, 379
Kzo9I GATC 1 cut(s) 355
LweI GCATC 1 cut(s) 112
MaeII ACGT 2 cut(s) 570, 619
MaeIII GTNAC 3 cut(s) 371, 622, 670
MalI GATC 1 cut(s) 357
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 355
MboII GAAGA 7 cut(s) 3, 16, 151, 308, 316, 410, 447
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 355
MluCI AATT 6 cut(s) 178, 189, 240, 398, 407, 714
MlyI GAGTC 2 cut(s) 266, 625
MmeI TCCRAC 2 cut(s) 99, 691
MseI TTAA 4 cut(s) 153, 183, 402, 440
MslI CAYNNNNRTG 2 cut(s) 203, 369
MspA1I CMGCKG 2 cut(s) 23, 341
MspR9I CCNGG 1 cut(s) 628
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 628
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 322
NdeII GATC 1 cut(s) 355
NlaIII CATG 3 cut(s) 208, 372, 379
NlaIV GGNNCC 3 cut(s) 163, 212, 447
NmuCI GTSAC 3 cut(s) 371, 622, 670
PagI TCATGA 1 cut(s) 204
PfeI GAWTC 1 cut(s) 636
PleI GAGTC 2 cut(s) 266, 625
PmaCI CACGTG 1 cut(s) 620
PmlI CACGTG 1 cut(s) 620
PpsI GAGTC 2 cut(s) 266, 625
Ppu21I YACGTR 1 cut(s) 620
Psp6I CCWGG 1 cut(s) 626
PspCI CACGTG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 3 cut(s) 163, 212, 447
PspPI GGNCC 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
RseI CAYNNNNRTG 2 cut(s) 203, 369
SaqAI TTAA 4 cut(s) 153, 183, 402, 440
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 266, 625
ScrFI CCNGG 1 cut(s) 628
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 510
SmiMI CAYNNNNRTG 2 cut(s) 203, 369
SmlI CTYRAG 2 cut(s) 50, 233
SmoI CTYRAG 2 cut(s) 50, 233
Sse9I AATT 6 cut(s) 178, 189, 240, 398, 407, 714
SsiI CCGC 3 cut(s) 40, 292, 341
StyD4I CCNGG 1 cut(s) 626
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 261, 511
TaiI ACGT 2 cut(s) 573, 622
TaqI TCGA 3 cut(s) 244, 634, 702
TasI AATT 6 cut(s) 178, 189, 240, 398, 407, 714
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 4 cut(s) 153, 183, 402, 440
Tru9I TTAA 4 cut(s) 153, 183, 402, 440
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 3 cut(s) 371, 622, 670
Tsp45I GTSAC 3 cut(s) 371, 622, 670
TspDTI ATGAA 5 cut(s) 17, 357, 400, 441, 688
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 1 cut(s) 514
XapI RAATTY 2 cut(s) 240, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.