AT3G25430

CAF1 family ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009492
Physical Location & Seq
Forward (+)
9221135 .. 9223866
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25430.2

Sequence Viewer

Length: 1857 bp
ATGCAAAGGCGCTTCCTTTCGTCAATCTCCGCCACCGCCGGCAATACTAAAACCCTAAATCAAGGAAGATGGAGTGTAAAGCAAGTGAAAAAATCCAACTTTCATGTAACTCTCGACGAGATTCGTACTTCCATCGATTCATCAGACTTCATAGCTCTCTCATTACAAAACACTGGCTCATACGCCGCCGCGTGGCACCGTGTCTCCGCCATTGACACGCCGCAAACTAGCTACTTAAAAGCTAAATACGCAGCCGAACGTTACCAGATTCTCCAGTTCGCTCTCTGCCCTTTCTCTCTTCAAGGCTCTAAACTCACTGTTCATCCATATAACTTTCATTTGTTTCCTCGTGATGAATTGAAGTGTGGTATGCCTTCTTATAGCTTCTCTTGTCAAGCTTCACGTTTAACAGCTATGGCTAGAGAAGGATTTGATTTCAACATTTGCATCTATGAAGGAATCTCATACTTATCTAGAGCACAAGAGTCTGCATCGAAGTTTCTTTCCGAGAATCCGATATTAGCTGATTCTGTTACTGTATCTTCTTCTCCAGCTACTGTAGCTGATACTGTCTTTGTTGGAAGGATTAGGTCACGTGTCAAGAATTGGAGACAGTCTTGTATAGACTCGGGTTCAAAGACAGGAGATGATGATTTGGTGAGCTCTTTGAGAAGGCTGGTGTTGGGGAGTGAGCAGTATGGTTCAAGGCTGTGTTTGACTATTGATGTTTGCAGTGAGCGTCAAGTGCAACTTATCCTAGAGATGTTGACTGAGTTCTCTGATGATGTTGTCCCTCTACTGGTCGCTTCAAAGAGCAGAGGAACACAAGCTGTTCGTACTGTCTTTATGAGTTCGAAGGAGGACAAGGATCTTTTTAAGAGAGAGCTTAAAGATCTTGAAAAGGAAGAAAACAGGCGAGTTCGTGGCTTTCGGGAAGTGGTCGATTTTATTTCTTCTTCACAGAAACCTGTCGTTTCCCAGAATTATCTTAGCGATTTTACTTCCATTCATGCTAAATTCCTTGGTCCTCTACCTTCAAATGTGGATGACTTCAGCAGTTCACTGAGCTCAGCATTTCCCAATGTTGTGGATCTCAGTCAGTTCATGAAAGAAATTAGTCCCTTAAGTAATATAAGTAATCTACCTGCAGCTATGTCCTCCTTGAATAGGTTCTTTGCACCTGTGGATGTAGAAGTAGCAAATCAAGGTTGTCCGGTCAAATTAGACGAAGGCCATCAGAGTCACGGGCAGAATGCTGTGATGATATCTCAGCTCTTTGCAAAACTGTGTACCATACAGAAATCAGATCTATCAACTATCCAATCAAATGAAGATTTCCAAGCTTTGGCTTCTGATGAACATGCAAATTCTGTCACCTCTTGCTCTAAAAACGCAGGTGATGAAAATGTTAAAGTCTGGTCAAAGAACAGCCGGAGAGTAAGCTCTGAGAACTTGGTGTTCATATGGGGGTTAGGGAAAAAGATGACAGCTGCAAAGCTGAAGAATGTCCTACAAAAGTCACACCCCGTCTTCGCGAGAGAGTTTGATGTTAAGTACATAGACCGGAGCTCTGCTATTTTGGTGTTTTGGGAGTCAGGTCCTTCAGAGACTTTCCTCAGTGCGGTCAACAATGAAGAGCAACTTGATGGTTCATTAAGAGAGATGGTTGCAGAAGGGCTAAGAGGGGCAGGTTACGAGACTTACAAGAGAGCTTGTAGATTAGGCTTCTGGGAGGCTGACTTGGCGGAATCTTTAGACAAAGCATTGGAATCTTCAGATACCGACCCTGATTCGGACACTAAACCATCAGAGATTGATTGGTCAAACGAATTAGCTATTAATTTTGATGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0043167 GO:0043169
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

618

Amino Acids

68.81

Weight (kDa)

5.68

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 29 - 384 2.1e-64 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1385
Acc36I ACCTGC 3 cut(s) 1153, 1385, 1679
AccB1I GGYRCC 1 cut(s) 195
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccII CGCG 2 cut(s) 191, 1535
AciI CCGC 8 cut(s) 30, 36, 186, 189, 207, 221, 1622, 1745
AclI AACGTT 1 cut(s) 259
AclWI GGATC 2 cut(s) 876, 1098
AcsI RAATTY 2 cut(s) 1016, 1366
AcuI CTGAAG 4 cut(s) 1036, 1520, 1587, 1758
AcvI CACGTG 1 cut(s) 596
AfaI GTAC 4 cut(s) 127, 838, 1291, 1556
AfiI CCNNNNNNNGG 6 cut(s) 192, 799, 1167, 1345, 1621, 1792
AflII CTTAAG 1 cut(s) 1123
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 9 cut(s) 302, 361, 439, 636, 705, 810, 899, 1038, 1165
Alw21I GWGCWC 4 cut(s) 481, 665, 1070, 1571
Alw26I GTCTC 4 cut(s) 208, 604, 1601, 1691
AlwI GGATC 2 cut(s) 876, 1098
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 1 cut(s) 1231
ApeKI GCWGC 3 cut(s) 251, 1148, 1490
ApoI RAATTY 2 cut(s) 1016, 1366
AseI ATTAAT 1 cut(s) 1839
Asp700I GAANNNNTTC 1 cut(s) 1169
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 1025, 1598
AsuHPI GGTGA 3 cut(s) 670, 1366, 1409
AsuII TTCGAA 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 628
AvaII GGWCC 2 cut(s) 1025, 1598
BanI GGYRCC 1 cut(s) 195
BanII GRGCYC 3 cut(s) 665, 1070, 1571
BauI CACGAG 1 cut(s) 348
BbrPI CACGTG 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 1522
Bbv12I GWGCWC 4 cut(s) 481, 665, 1070, 1571
BbvI GCAGC 3 cut(s) 263, 1160, 1477
BccI CCATC 6 cut(s) 63, 140, 1242, 1640, 1657, 1813
BcoDI GTCTC 4 cut(s) 208, 604, 1601, 1691
BfaI CTAG 4 cut(s) 228, 420, 474, 758
BfmI CTRYAG 2 cut(s) 558, 1146
BfoI RGCGCY 1 cut(s) 13
BfrI CTTAAG 1 cut(s) 1123
BfuAI ACCTGC 3 cut(s) 1153, 1385, 1679
BglII AGATCT 2 cut(s) 892, 1306
BisI GCNGC 6 cut(s) 186, 189, 221, 252, 1149, 1491
BlpI GCTNAGC 1 cut(s) 1069
BlsI GCNGC 6 cut(s) 187, 190, 222, 253, 1150, 1492
Bme18I GGWCC 2 cut(s) 1025, 1598
BmeT110I CYCGRG 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 1025, 1598
BmiI GGNNCC 1 cut(s) 197
BmsI GCATC 2 cut(s) 456, 500
BpiI GAAGAC 1 cut(s) 1522
BpmI CTGGAG 2 cut(s) 257, 534
Bpu1102I GCTNAGC 1 cut(s) 1069
Bpu14I TTCGAA 1 cut(s) 854
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 1021
BsaWI WCCGGW 2 cut(s) 1213, 1563
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 6 cut(s) 192, 799, 1167, 1345, 1621, 1792
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 3 cut(s) 178, 274, 804
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 1021
BseGI GGATG 3 cut(s) 322, 1051, 1192
BseLI CCNNNNNNNGG 6 cut(s) 192, 799, 1167, 1345, 1621, 1792
BseMII CTCAG 7 cut(s) 762, 1055, 1083, 1108, 1283, 1437, 1630
BseNI ACTGG 3 cut(s) 178, 274, 804
BseXI GCAGC 3 cut(s) 263, 1160, 1477
Bsh1236I CGCG 2 cut(s) 191, 1535
BshFI GGCC 1 cut(s) 1233
BshNI GGYRCC 1 cut(s) 195
BshVI ATCGAT 1 cut(s) 135
BsiHKAI GWGCWC 4 cut(s) 481, 665, 1070, 1571
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 4 cut(s) 39, 1214, 1432, 1564
BslFI GGGAC 2 cut(s) 776, 1104
BslI CCNNNNNNNGG 6 cut(s) 192, 799, 1167, 1345, 1621, 1792
BsmAI GTCTC 4 cut(s) 208, 604, 1601, 1691
BsmFI GGGAC 2 cut(s) 776, 1104
BsmI GAATGC 1 cut(s) 1258
BsnI GGCC 1 cut(s) 1233
BsoBI CYCGRG 1 cut(s) 628
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 4 cut(s) 481, 665, 1070, 1571
Bsp143I GATC 4 cut(s) 868, 892, 1090, 1306
Bsp1720I GCTNAGC 1 cut(s) 1069
Bsp68I TCGCGA 1 cut(s) 1535
BspACI CCGC 8 cut(s) 30, 36, 186, 189, 207, 221, 1622, 1745
BspANI GGCC 1 cut(s) 1233
BspCNI CTCAG 7 cut(s) 763, 1056, 1082, 1107, 1282, 1438, 1629
BspDI ATCGAT 1 cut(s) 135
BspFNI CGCG 2 cut(s) 191, 1535
BspHI TCATGA 1 cut(s) 1104
BspLI GGNNCC 1 cut(s) 197
BspMAI CTGCAG 1 cut(s) 1150
BspMI ACCTGC 3 cut(s) 1153, 1385, 1679
BspPI GGATC 2 cut(s) 876, 1098
BspQI GCTCTTC 1 cut(s) 1629
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 195
BspTI CTTAAG 1 cut(s) 1123
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 3 cut(s) 178, 274, 804
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 1021
BssMI GATC 4 cut(s) 868, 892, 1090, 1306
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 1021
Bst2BI CACGAG 1 cut(s) 348
Bst4CI ACNGT 8 cut(s) 200, 319, 538, 559, 571, 615, 841, 1287
Bst6I CTCTTC 2 cut(s) 303, 1629
BstAFI CTTAAG 1 cut(s) 1123
BstBAI YACGTR 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 9 cut(s) 771, 989, 1064, 1069, 1094, 1269, 1446, 1616, 1679
BstENI CCTNNNNNAGG 1 cut(s) 1165
BstF5I GGATG 3 cut(s) 322, 1051, 1192
BstFNI CGCG 2 cut(s) 191, 1535
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 4 cut(s) 871, 895, 1093, 1309
BstMAI GTCTC 4 cut(s) 208, 604, 1601, 1691
BstMBI GATC 4 cut(s) 868, 892, 1090, 1306
BstMWI GCNNNNNNNGC 4 cut(s) 248, 560, 745, 1742
BstNSI RCATGY 1 cut(s) 1364
BstSFI CTRYAG 2 cut(s) 558, 1146
BstUI CGCG 2 cut(s) 191, 1535
BstV1I GCAGC 3 cut(s) 263, 1160, 1477
BstV2I GAAGAC 1 cut(s) 1522
BstX2I RGATCY 4 cut(s) 868, 892, 1090, 1306
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 4 cut(s) 868, 892, 1090, 1306
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 1 cut(s) 1233
BsuTUI ATCGAT 1 cut(s) 135
BtsCI GGATG 3 cut(s) 322, 1051, 1192
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 5 cut(s) 171, 315, 739, 1061, 1624
BtuMI TCGCGA 1 cut(s) 1535
BveI ACCTGC 3 cut(s) 1153, 1385, 1679
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 557
CciI TCATGA 1 cut(s) 1104
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 2 cut(s) 1025, 1598
ClaI ATCGAT 1 cut(s) 135
CseI GACGC 1 cut(s) 728
Csp6I GTAC 4 cut(s) 126, 837, 1290, 1555
CviAII CATG 4 cut(s) 104, 1010, 1105, 1361
CviQI GTAC 4 cut(s) 126, 837, 1290, 1555
DdeI CTNAG 9 cut(s) 771, 989, 1064, 1069, 1094, 1269, 1446, 1616, 1679
DpnI GATC 4 cut(s) 870, 894, 1092, 1308
DpnII GATC 4 cut(s) 868, 892, 1090, 1306
Eam1104I CTCTTC 2 cut(s) 303, 1629
EarI CTCTTC 2 cut(s) 303, 1629
EciI GGCGGA 3 cut(s) 19, 196, 1760
Ecl136II GAGCTC 3 cut(s) 663, 1068, 1569
Eco130I CCWWGG 1 cut(s) 1021
Eco24I GRGCYC 3 cut(s) 665, 1070, 1571
Eco32I GATATC 1 cut(s) 1266
Eco47I GGWCC 2 cut(s) 1025, 1598
Eco53kI GAGCTC 3 cut(s) 663, 1068, 1569
Eco57I CTGAAG 4 cut(s) 1036, 1520, 1587, 1758
Eco72I CACGTG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 628
EcoICRI GAGCTC 3 cut(s) 663, 1068, 1569
EcoNI CCTNNNNNAGG 1 cut(s) 1165
EcoO109I RGGNCCY 1 cut(s) 1598
EcoRV GATATC 1 cut(s) 1266
EcoT14I CCWWGG 1 cut(s) 1021
EcoT38I GRGCYC 3 cut(s) 665, 1070, 1571
ErhI CCWWGG 1 cut(s) 1021
FaeI CATG 4 cut(s) 107, 1013, 1108, 1364
FalI AAGNNNNNCTT 4 cut(s) 735, 767, 1626, 1658
FaqI GGGAC 2 cut(s) 776, 1104
FatI CATG 4 cut(s) 103, 1009, 1104, 1360
FauNDI CATATG 1 cut(s) 1463
Fnu4HI GCNGC 6 cut(s) 186, 189, 221, 252, 1149, 1491
FokI GGATG 3 cut(s) 309, 1058, 1199
FriOI GRGCYC 3 cut(s) 665, 1070, 1571
Fsp4HI GCNGC 6 cut(s) 186, 189, 221, 252, 1149, 1491
FspBI CTAG 4 cut(s) 228, 420, 474, 758
GlaI GCGC 1 cut(s) 11
GluI GCNGC 6 cut(s) 186, 189, 221, 252, 1149, 1491
GsuI CTGGAG 2 cut(s) 257, 534
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 1 cut(s) 1233
HapII CCGG 4 cut(s) 39, 1214, 1432, 1564
HgaI GACGC 1 cut(s) 728
HhaI GCGC 1 cut(s) 12
Hin1II CATG 4 cut(s) 107, 1013, 1108, 1364
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 2 cut(s) 768, 1627
HindII GTYRAC 2 cut(s) 768, 1627
HindIII AAGCTT 2 cut(s) 396, 1341
HpaII CCGG 4 cut(s) 39, 1214, 1432, 1564
HphI GGTGA 3 cut(s) 670, 1366, 1409
Hpy166II GTNNAC 4 cut(s) 768, 1061, 1290, 1627
Hpy188III TCNNGA 8 cut(s) 113, 350, 474, 601, 896, 932, 1105, 1534
Hpy8I GTNNAC 4 cut(s) 768, 1061, 1290, 1627
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 8 cut(s) 200, 319, 538, 559, 571, 615, 841, 1287
HpyCH4IV ACGT 3 cut(s) 259, 403, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 560, 745, 1742
HpyF3I CTNAG 9 cut(s) 771, 989, 1064, 1069, 1094, 1269, 1446, 1616, 1679
HpySE526I ACGT 3 cut(s) 259, 403, 595
Hsp92II CATG 4 cut(s) 107, 1013, 1108, 1364
HspAI GCGC 1 cut(s) 10
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Kzo9I GATC 4 cut(s) 868, 892, 1090, 1306
LguI GCTCTTC 1 cut(s) 1629
LmnI GCTCC 1 cut(s) 1566
Lsp1109I GCAGC 3 cut(s) 263, 1160, 1477
LweI GCATC 2 cut(s) 456, 500
MaeI CTAG 4 cut(s) 228, 420, 474, 758
MaeII ACGT 3 cut(s) 259, 403, 595
MaeIII GTNAC 8 cut(s) 106, 260, 532, 591, 1241, 1372, 1518, 1691
MalI GATC 4 cut(s) 870, 894, 1092, 1308
MboI GATC 4 cut(s) 868, 892, 1090, 1306
MflI RGATCY 4 cut(s) 868, 892, 1090, 1306
MhlI GDGCHC 4 cut(s) 481, 665, 1070, 1571
MluCI AATT 9 cut(s) 356, 604, 982, 1016, 1113, 1220, 1366, 1829, 1840
MlyI GAGTC 4 cut(s) 494, 620, 1249, 1601
MmeI TCCRAC 2 cut(s) 120, 559
MroNI GCCGGC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 9 cut(s) 236, 407, 876, 888, 1124, 1410, 1551, 1655, 1839
MspA1I CMGCKG 1 cut(s) 1490
MspCI CTTAAG 1 cut(s) 1123
MspI CCGG 4 cut(s) 39, 1214, 1432, 1564
Mva1269I GAATGC 1 cut(s) 1258
MvnI CGCG 2 cut(s) 191, 1535
MwoI GCNNNNNNNGC 4 cut(s) 248, 560, 745, 1742
NaeI GCCGGC 1 cut(s) 40
NdeI CATATG 1 cut(s) 1463
NdeII GATC 4 cut(s) 868, 892, 1090, 1306
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 4 cut(s) 107, 1013, 1108, 1364
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 4 cut(s) 591, 1241, 1372, 1518
NruI TCGCGA 1 cut(s) 1535
NspI RCATGY 1 cut(s) 1364
NspV TTCGAA 1 cut(s) 854
PagI TCATGA 1 cut(s) 1104
PaqCI CACCTGC 1 cut(s) 1385
PciSI GCTCTTC 1 cut(s) 1629
PctI GAATGC 1 cut(s) 1258
PdiI GCCGGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 1169
PfeI GAWTC 9 cut(s) 121, 137, 268, 459, 511, 527, 1748, 1769, 1790
PflMI CCANNNNNTGG 1 cut(s) 1345
PkrI GCNGC 6 cut(s) 187, 190, 222, 253, 1150, 1492
PleI GAGTC 4 cut(s) 493, 620, 1248, 1600
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 4 cut(s) 493, 620, 1248, 1600
Ppu21I YACGTR 1 cut(s) 596
PpuMI RGGWCCY 1 cut(s) 1598
PshBI ATTAAT 1 cut(s) 1839
Psp124BI GAGCTC 3 cut(s) 665, 1070, 1571
Psp1406I AACGTT 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 1598
PspCI CACGTG 1 cut(s) 596
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 2 cut(s) 1025, 1598
PspPPI RGGWCCY 1 cut(s) 1598
PstI CTGCAG 1 cut(s) 1150
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 4 cut(s) 868, 892, 1090, 1306
PvuII CAGCTG 1 cut(s) 1490
RruI TCGCGA 1 cut(s) 1535
RsaI GTAC 4 cut(s) 127, 838, 1291, 1556
RsaNI GTAC 4 cut(s) 126, 837, 1290, 1555
SacI GAGCTC 3 cut(s) 665, 1070, 1571
SapI GCTCTTC 1 cut(s) 1629
SaqAI TTAA 9 cut(s) 236, 407, 876, 888, 1124, 1410, 1551, 1655, 1839
SatI GCNGC 6 cut(s) 186, 189, 221, 252, 1149, 1491
Sau3AI GATC 4 cut(s) 868, 892, 1090, 1306
Sau96I GGNCC 2 cut(s) 1025, 1598
SchI GAGTC 4 cut(s) 494, 620, 1249, 1601
SduI GDGCHC 4 cut(s) 481, 665, 1070, 1571
SfaNI GCATC 2 cut(s) 456, 500
SfcI CTRYAG 2 cut(s) 558, 1146
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 2 cut(s) 1025, 1598
SmlI CTYRAG 1 cut(s) 1123
SmoI CTYRAG 1 cut(s) 1123
Sse9I AATT 9 cut(s) 356, 604, 982, 1016, 1113, 1220, 1366, 1829, 1840
SsiI CCGC 8 cut(s) 30, 36, 186, 189, 207, 221, 1622, 1745
SspMI CTAG 4 cut(s) 228, 420, 474, 758
SstI GAGCTC 3 cut(s) 665, 1070, 1571
StyI CCWWGG 1 cut(s) 1021
TaaI ACNGT 8 cut(s) 200, 319, 538, 559, 571, 615, 841, 1287
TaiI ACGT 3 cut(s) 262, 406, 598
TaqI TCGA 5 cut(s) 114, 135, 494, 854, 942
TasI AATT 9 cut(s) 356, 604, 982, 1016, 1113, 1220, 1366, 1829, 1840
TatI WGTACW 1 cut(s) 1554
TauI GCSGC 3 cut(s) 188, 191, 223
TfiI GAWTC 9 cut(s) 121, 137, 268, 459, 511, 527, 1748, 1769, 1790
Tru1I TTAA 9 cut(s) 236, 407, 876, 888, 1124, 1410, 1551, 1655, 1839
Tru9I TTAA 9 cut(s) 236, 407, 876, 888, 1124, 1410, 1551, 1655, 1839
TscAI CASTG 5 cut(s) 178, 322, 739, 1068, 1624
TseFI GTSAC 4 cut(s) 591, 1241, 1372, 1518
TseI GCWGC 3 cut(s) 251, 1148, 1490
Tsp45I GTSAC 4 cut(s) 591, 1241, 1372, 1518
TspRI CASTG 5 cut(s) 178, 322, 739, 1068, 1624
Van91I CCANNNNNTGG 1 cut(s) 1345
Vha464I CTTAAG 1 cut(s) 1123
VpaK11BI GGWCC 2 cut(s) 1025, 1598
VspI ATTAAT 1 cut(s) 1839
XagI CCTNNNNNAGG 1 cut(s) 1165
XapI RAATTY 2 cut(s) 1016, 1366
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 1 cut(s) 1364
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 4 cut(s) 228, 420, 474, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.