MD05G1157500.v1.1

Poly(A)-specific ribonuclease PARN-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009492
Physical Location & Seq
Reverse (-)
28695621 .. 28697687
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1157500.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGCCTGCATATATAATGACGGGTATTTCATACTTGTCTGGAGCACAAGAATCTGCGGCTAAAGCTCGGATAGGGAATCCGACTACCAGTAGCTATGTAATGAACTCTTCTTCGACTCATACTGTTGCTGATACGGTTTTTGTTGAAAGAATTAAATTGCGAGTTAATCATTGGAAAAGTGCATGTAAAAACACAAAGAAAGATGGTAAAGACAGAAAAAGGGTTCTAGATTCTACTTACATCCCCTATCTTTCATTCGATTTTATGTTGAAAGAGTTCTCTGATGAACTTGTTCCTTTAATAATCCCAGCAAAGGGAGGAGGAACTCAGGCTGTTCGTGCTGTTTTGACAAGTTCTACAGCAGACAAGGACTGGTTTGAGAGAGAGCTCCAAAACCTTGAGGAGGAACAAAACAAGAGAGTTCGTGGATTTCAAGAGGTGATCGATTTGATTTCTGCTTCACAGAAACCTGTTGTCTCCCACAATTCCCTTAACGGTATTTCAGTTATTCATTCAGAGTTTCTCTCTCCCCTCCCGACTAATGTAGACGAGTTCATGGGTTCCTTGCACTCGGTTTTTCCCCACATACTTGATGTCAACCATCTGATGAAGAATATTGGCCCTCTAAGAAGGATGACCAATATTCCTGCAGCTATTTCGTACTTGAATAATCATTACTTCGCAGCCATTGATATGGAAATTTTGCCTCAAGGTATCGATATTACCCTTAGTTTTCGCGTAGCACAGTTGAAGAGAGTGCATGAAGGCAACATTCAAAGGCATAATGTGGTGAAGATGTGCTATTTGTTTGCAAAGCTCTGCTCCATTCTGAAAATTTCCGACAACGCTATGGTATCTAACAATGCGATGTTGGGGATGATCAAGAGCTTGTTGTGCAAGTTACATGCTGTCTTCTCTGAAGAATTTGATGTTCGGTTATTGGACAAGAGCTGTGCCATTGTTGTTTTCTGGCGACGTGGTTTATCTGAAACTTTTTTGGATGCCATGAGCAGTGAAGAGATTTGTGACTCTTTAATGGAGATCGCTTCGGAGGGCATAAGGGCATCAAGTTACGAAACTTACAAGAAAGTCTGCAGGTTAGGCTTATGGGAGGCAGATATGGCGGAGTCCCTTGACAAAGCCTTGGAAGACTTGGACTACCTTACGGAAGTTAATTCAACAACAAATTCTAAAGAAATTTATTGGTCTAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0043167 GO:0043169
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

406

Amino Acids

45.57

Weight (kDa)

6.15

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 5 - 215 1.7e-23 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1086
AccI GTMKAC 1 cut(s) 546
AccII CGCG 1 cut(s) 738
AciI CCGC 2 cut(s) 56, 1124
AcsI RAATTY 5 cut(s) 699, 834, 923, 1186, 1197
AcuI CTGAAG 1 cut(s) 939
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 3 cut(s) 313, 314, 403
AgsI TTSAA 7 cut(s) 146, 271, 434, 667, 751, 776, 1179
AjiI CACGTC 1 cut(s) 977
AluBI AGCT 7 cut(s) 65, 93, 388, 653, 817, 888, 951
AluI AGCT 7 cut(s) 65, 93, 388, 653, 817, 888, 951
Alw21I GWGCWC 2 cut(s) 46, 390
Alw26I GTCTC 1 cut(s) 481
AoxI GGCC 1 cut(s) 619
ApeKI GCWGC 2 cut(s) 650, 683
ApoI RAATTY 5 cut(s) 699, 834, 923, 1186, 1197
Asp700I GAANNNNTTC 2 cut(s) 275, 291
AspS9I GGNCC 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 451, 802
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 2 cut(s) 904, 1155
Bbv12I GWGCWC 2 cut(s) 46, 390
BbvI GCAGC 2 cut(s) 662, 695
BccI CCATC 2 cut(s) 197, 609
BcgI CGANNNNNNTGC 2 cut(s) 639, 673
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 2 cut(s) 227, 1209
BfmI CTRYAG 3 cut(s) 357, 648, 1093
BfuAI ACCTGC 1 cut(s) 1086
BisI GCNGC 3 cut(s) 57, 651, 684
BlsI GCNGC 3 cut(s) 58, 652, 685
BmgBI CACGTC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 1 cut(s) 562
BmsI GCATC 2 cut(s) 991, 1073
BpiI GAAGAC 2 cut(s) 904, 1155
BplI GAGNNNNNCTC 2 cut(s) 509, 541
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 2 cut(s) 419, 693
Bsa29I ATCGAT 2 cut(s) 444, 717
BsaJI CCNNGG 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 314, 403
Bse1I ACTGG 2 cut(s) 87, 377
BseCI ATCGAT 2 cut(s) 444, 717
BseDI CCNNGG 1 cut(s) 1143
BseGI GGATG 4 cut(s) 240, 639, 882, 1006
BseLI CCNNNNNNNGG 3 cut(s) 313, 314, 403
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 87, 377
BseRI GAGGAG 2 cut(s) 333, 416
BseXI GCAGC 2 cut(s) 662, 695
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 738
BshFI GGCC 1 cut(s) 621
BshVI ATCGAT 2 cut(s) 444, 717
BsiHKAI GWGCWC 2 cut(s) 46, 390
BslFI GGGAC 1 cut(s) 1114
BslI CCNNNNNNNGG 3 cut(s) 313, 314, 403
BsmAI GTCTC 1 cut(s) 481
BsmFI GGGAC 1 cut(s) 1114
BsnI GGCC 1 cut(s) 621
Bsp1286I GDGCHC 2 cut(s) 46, 390
Bsp143I GATC 3 cut(s) 441, 879, 1041
BspACI CCGC 2 cut(s) 56, 1124
BspANI GGCC 1 cut(s) 621
BspCNI CTCAG 1 cut(s) 340
BspDI ATCGAT 2 cut(s) 444, 717
BspFNI CGCG 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 562
BspMAI CTGCAG 2 cut(s) 652, 1097
BspMI ACCTGC 1 cut(s) 1086
BsrI ACTGG 2 cut(s) 87, 377
BssECI CCNNGG 1 cut(s) 1143
BssMI GATC 3 cut(s) 441, 879, 1041
BssT1I CCWWGG 1 cut(s) 1143
Bst4CI ACNGT 4 cut(s) 124, 136, 497, 747
Bst6I CTCTTC 3 cut(s) 112, 746, 1011
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 327, 626, 728
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 4 cut(s) 240, 639, 882, 1006
BstFNI CGCG 1 cut(s) 738
BstKTI GATC 3 cut(s) 444, 882, 1044
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 3 cut(s) 441, 879, 1041
BstMWI GCNNNNNNNGC 5 cut(s) 62, 338, 894, 1101, 1121
BstNSI RCATGY 2 cut(s) 186, 908
BstSFI CTRYAG 3 cut(s) 357, 648, 1093
BstUI CGCG 1 cut(s) 738
BstV1I GCAGC 2 cut(s) 662, 695
BstV2I GAAGAC 2 cut(s) 904, 1155
BstXI CCANNNNNNTGG 1 cut(s) 694
Bsu15I ATCGAT 2 cut(s) 444, 717
BsuRI GGCC 1 cut(s) 621
BsuTUI ATCGAT 2 cut(s) 444, 717
BtgZI GCGATG 1 cut(s) 881
BtrI CACGTC 1 cut(s) 977
BtsCI GGATG 4 cut(s) 240, 639, 882, 1006
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 1 cut(s) 1018
BveI ACCTGC 1 cut(s) 1086
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 620
ClaI ATCGAT 2 cut(s) 444, 717
Csp6I GTAC 1 cut(s) 661
CviAII CATG 5 cut(s) 183, 556, 761, 905, 1006
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 3 cut(s) 327, 626, 728
DpnI GATC 3 cut(s) 443, 881, 1043
DpnII GATC 3 cut(s) 441, 879, 1041
Eam1104I CTCTTC 3 cut(s) 112, 746, 1011
EarI CTCTTC 3 cut(s) 112, 746, 1011
EciI GGCGGA 1 cut(s) 1139
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 939
EcoICRI GAGCTC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 1143
FaeI CATG 5 cut(s) 186, 559, 764, 908, 1009
FaqI GGGAC 1 cut(s) 1114
FatI CATG 5 cut(s) 182, 555, 760, 904, 1005
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 3 cut(s) 57, 651, 684
FokI GGATG 4 cut(s) 227, 646, 889, 1013
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 3 cut(s) 57, 651, 684
FspBI CTAG 2 cut(s) 227, 1209
GluI GCNGC 3 cut(s) 57, 651, 684
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 621
Hin1II CATG 5 cut(s) 186, 559, 764, 908, 1009
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 6 cut(s) 50, 76, 115, 230, 1028, 1127
HphI GGTGA 2 cut(s) 451, 802
Hpy166II GTNNAC 2 cut(s) 547, 598
Hpy188III TCNNGA 5 cut(s) 39, 227, 434, 535, 883
Hpy8I GTNNAC 2 cut(s) 547, 598
Hpy99I CGWCG 1 cut(s) 978
HpyAV CCTTC 2 cut(s) 624, 758
HpyCH4III ACNGT 4 cut(s) 124, 136, 497, 747
HpyCH4IV ACGT 1 cut(s) 976
HpyCH4V TGCA 8 cut(s) 8, 182, 568, 650, 760, 812, 897, 1095
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 338, 894, 1101, 1121
HpyF3I CTNAG 3 cut(s) 327, 626, 728
HpySE526I ACGT 1 cut(s) 976
Hsp92II CATG 5 cut(s) 186, 559, 764, 908, 1009
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 3 cut(s) 441, 879, 1041
LmnI GCTCC 3 cut(s) 41, 393, 827
Lsp1109I GCAGC 2 cut(s) 662, 695
LweI GCATC 2 cut(s) 991, 1073
MaeI CTAG 2 cut(s) 227, 1209
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 3 cut(s) 900, 1025, 1070
MalI GATC 3 cut(s) 443, 881, 1043
MboI GATC 3 cut(s) 441, 879, 1041
MboII GAAGA 9 cut(s) 99, 102, 622, 763, 805, 904, 932, 1028, 1160
MhlI GDGCHC 2 cut(s) 46, 390
MluCI AATT 9 cut(s) 150, 155, 484, 699, 834, 923, 1174, 1186, 1197
MlyI GAGTC 3 cut(s) 109, 1022, 1136
MmeI TCCRAC 2 cut(s) 104, 864
MroXI GAANNNNTTC 2 cut(s) 275, 291
MseI TTAA 6 cut(s) 153, 165, 299, 492, 1034, 1173
MslI CAYNNNNRTG 1 cut(s) 692
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 5 cut(s) 62, 338, 894, 1101, 1121
NdeII GATC 3 cut(s) 441, 879, 1041
NlaIII CATG 5 cut(s) 186, 559, 764, 908, 1009
NlaIV GGNNCC 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 1025
NspI RCATGY 2 cut(s) 186, 908
PdmI GAANNNNTTC 2 cut(s) 275, 291
PfeI GAWTC 3 cut(s) 50, 76, 230
PkrI GCNGC 3 cut(s) 58, 652, 685
PleI GAGTC 3 cut(s) 109, 1022, 1135
PpsI GAGTC 3 cut(s) 109, 1022, 1135
Psp124BI GAGCTC 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 562
PspPI GGNCC 1 cut(s) 620
PstI CTGCAG 2 cut(s) 652, 1097
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 390
SaqAI TTAA 6 cut(s) 153, 165, 299, 492, 1034, 1173
SatI GCNGC 3 cut(s) 57, 651, 684
Sau3AI GATC 3 cut(s) 441, 879, 1041
Sau96I GGNCC 1 cut(s) 620
SchI GAGTC 3 cut(s) 109, 1022, 1136
SduI GDGCHC 2 cut(s) 46, 390
SfaNI GCATC 2 cut(s) 991, 1073
SfcI CTRYAG 3 cut(s) 357, 648, 1093
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 398, 708
SmoI CTYRAG 2 cut(s) 398, 708
Sse9I AATT 9 cut(s) 150, 155, 484, 699, 834, 923, 1174, 1186, 1197
SsiI CCGC 2 cut(s) 56, 1124
SspI AATATT 2 cut(s) 616, 643
SspMI CTAG 2 cut(s) 227, 1209
SstI GAGCTC 1 cut(s) 390
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 4 cut(s) 124, 136, 497, 747
TaiI ACGT 1 cut(s) 979
TaqI TCGA 4 cut(s) 113, 258, 444, 717
TasI AATT 9 cut(s) 150, 155, 484, 699, 834, 923, 1174, 1186, 1197
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 3 cut(s) 50, 76, 230
Tru1I TTAA 6 cut(s) 153, 165, 299, 492, 1034, 1173
Tru9I TTAA 6 cut(s) 153, 165, 299, 492, 1034, 1173
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 1 cut(s) 1025
TseI GCWGC 2 cut(s) 650, 683
Tsp45I GTSAC 1 cut(s) 1025
TspDTI ATGAA 8 cut(s) 18, 116, 243, 300, 500, 544, 623, 777
TspGWI ACGGA 1 cut(s) 1181
TspRI CASTG 1 cut(s) 1018
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 5 cut(s) 699, 834, 923, 1186, 1197
XbaI TCTAGA 1 cut(s) 226
XceI RCATGY 2 cut(s) 186, 908
XmiI GTMKAC 1 cut(s) 546
XmnI GAANNNNTTC 2 cut(s) 275, 291
XspI CTAG 2 cut(s) 227, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.