MD05G1157600.v1.1

Poly(A)-specific ribonuclease PARN-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009492
Physical Location & Seq
Reverse (-)
28697689 .. 28698126
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1157600.v1.1.491

Sequence Viewer

Length: 291 bp
ATGGGCCGTGAAGGAAGTGACGAAGTCCAACTTTTCCGAGTCCCTGGAGAGTTCAAGGACCACCTGTCCAGCTCCGACTTCGTGGCCTTCTCGCTGCAGAAAACGGGGTCATATCGTGCGCCGTGGCGCCACCCTCAGCCCTTCGACACCGCCGACACTTCGTATTTCAAGGCCAAGTACGCCGCTGAGCGCTTCCAGCTCCTCCAGTTCGCAGCCTGCCCTTACACTCTCAGAGCCTCGAAGCTTACTCCTTACCCGTCCATACAACTTTGTCTTGTTCCCAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0043167 GO:0043169
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

97

Amino Acids

10.9

Weight (kDa)

9.0

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 17 - 81 2.3e-10 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AciI CCGC 2 cut(s) 150, 183
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 1 cut(s) 179
AfeI AGCGCT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 55, 169
AhdI GACNNNNNGTC 1 cut(s) 64
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 3 cut(s) 72, 199, 244
AluI AGCT 3 cut(s) 72, 199, 244
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 3 cut(s) 4, 84, 171
ApeKI GCWGC 2 cut(s) 94, 212
AspLEI GCGC 3 cut(s) 121, 129, 192
AspS9I GGNCC 2 cut(s) 4, 58
AvaII GGWCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 81, 224
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 95
BfoI RGCGCY 2 cut(s) 130, 193
BisI GCNGC 3 cut(s) 95, 183, 213
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 3 cut(s) 96, 184, 214
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 58
BmeRI GACNNNNNGTC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 4, 58
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 45
BpmI CTGGAG 2 cut(s) 66, 188
Bpu10I CCTNAGC 1 cut(s) 135
Bpu1102I GCTNAGC 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 3 cut(s) 43, 122, 282
Bse1I ACTGG 1 cut(s) 205
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 43, 122, 282
BseMII CTCAG 3 cut(s) 149, 177, 244
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 81, 224
BshFI GGCC 3 cut(s) 6, 86, 173
BshNI GGYRCC 1 cut(s) 126
BslFI GGGAC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 3 cut(s) 6, 86, 173
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 2 cut(s) 150, 183
BspANI GGCC 3 cut(s) 6, 86, 173
BspCNI CTCAG 3 cut(s) 148, 178, 243
BspLI GGNNCC 1 cut(s) 128
BspMAI CTGCAG 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 43, 122, 282
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 3 cut(s) 135, 186, 230
BstDSI CCRYGG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 130, 193
BstHHI GCGC 3 cut(s) 121, 129, 192
BstMWI GCNNNNNNNGC 2 cut(s) 179, 196
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 81, 224
BsuRI GGCC 3 cut(s) 6, 86, 173
BtgI CCRYGG 1 cut(s) 122
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 3 cut(s) 121, 129, 192
Cfr13I GGNCC 2 cut(s) 4, 58
Csp6I GTAC 1 cut(s) 178
CviJI RGCY 9 cut(s) 6, 72, 86, 139, 173, 199, 215, 236, 244
CviKI_1 RGCY 9 cut(s) 6, 72, 86, 139, 173, 199, 215, 236, 244
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 3 cut(s) 135, 186, 230
DinI GGCGCC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 64
Eam1105I GACNNNNNGTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 58
Eco47III AGCGCT 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 282
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 282
FaiI YATR 2 cut(s) 112, 263
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 95, 183, 213
Fsp4HI GCNGC 3 cut(s) 95, 183, 213
GlaI GCGC 3 cut(s) 120, 128, 191
GluI GCNGC 3 cut(s) 95, 183, 213
GsuI CTGGAG 2 cut(s) 66, 188
HaeII RGCGCY 2 cut(s) 130, 193
HaeIII GGCC 3 cut(s) 6, 86, 173
HhaI GCGC 3 cut(s) 121, 129, 192
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 3 cut(s) 119, 127, 190
HinP1I GCGC 3 cut(s) 119, 127, 190
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 1 cut(s) 39
Hpy188I TCNGA 3 cut(s) 38, 76, 233
HpyAV CCTTC 3 cut(s) 5, 97, 151
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 196
HpyF3I CTNAG 3 cut(s) 135, 186, 230
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 3 cut(s) 119, 127, 190
KasI GGCGCC 1 cut(s) 126
LmnI GCTCC 2 cut(s) 77, 204
LpnPI CCDG 7 cut(s) 30, 57, 77, 82, 209, 218, 229
Lsp1109I GCAGC 2 cut(s) 81, 224
MaeIII GTNAC 1 cut(s) 17
Mly113I GGCGCC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 2 cut(s) 52, 99
MnlI CCTC 3 cut(s) 144, 212, 247
MspA1I CMGCKG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 179, 196
NarI GGCGCC 1 cut(s) 127
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 23
PkrI GCNGC 3 cut(s) 96, 184, 214
PleI GAGTC 1 cut(s) 47
PluTI GGCGCC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 4, 58
PstI CTGCAG 1 cut(s) 99
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 3 cut(s) 95, 183, 213
Sau96I GGNCC 2 cut(s) 4, 58
SchI GAGTC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 45
SetI ASST 4 cut(s) 66, 74, 201, 246
SfcI CTRYAG 1 cut(s) 95
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 1 cut(s) 58
SsiI CCGC 2 cut(s) 150, 183
SspDI GGCGCC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 282
TaqI TCGA 2 cut(s) 144, 239
TauI GCSGC 1 cut(s) 185
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 2 cut(s) 94, 212
Tsp45I GTSAC 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.