FvH4_2g17670

Poly(A)-specific ribonuclease PARN-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009492
Physical Location & Seq
Reverse (-)
15187732 .. 15194850
7119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17670.t1

Sequence Viewer

Length: 1842 bp
ATGGCGGCTCTGATACGGCGGCGTTTGCTCTGCACGAGCGCCGAGGCGGCGGGGAGAAAGCTGGCGGTGAGGGAGGTGACGAAGGCGAATTTCGCGGAGGCGGTGGAGGAGTTCAAGGAGGACCTGGCCGGGTCGGACTTTGTTGCCGTGTCGCTGCAGAAAACGGGGTCGTTTTCGGCGGGGTGGCACCGGGAGCTGCCGTTTGATACAAACGACACGGCGTATTTGAAGGCGAAGTACGCGGCGGAGCGTTTCCAGGTGCTGCAGTTCGCCGCCTGCCCTTTCAGTATCAGAGCCTCCAAGCTCACCGCTCAGCCATATAACTTTGTGTTGTTTCCTAGAGATGAACTGAAGATGGGGATGCCATCTTATAGTTTTTCAATTCAAACGTCACATATGGCATACTTGGCTCAAGAAGGTTTTGATTTTAATGCCTGCATTTATGATGGTATATCGTACTTATCTAAAGCACAAGAATCTGCGGCTAAAGTGCGGATAGGGAATCCAACGCCTGGCAACTATGTAGTAGATTCTAAAGTAACCCATACTGTTGCTGATACAATGTTTATTGAGAGAACTAAATCACGAGTTAAGAATTGGAAAAATACGTGCAAGAACTCTAGTTCAAGGACAGATGATGCTCTGCTGAGTTCCTTGAGGAAACTTGTGATGGGGACTGAACTTTATGGATCGAGACCATGCATGAATATAGATGTTTGCAGTGAGCGTCAAGTACAGCTTTCACTGGAGATGCTAAAAGAGTTCAGTGATGAACTTGTTCCTGTAATAGTCCCAGCAAAGGGTGGGGGTACTCAGTCTGTTCGTGTTTTTTTGACAAGTTCCAAAGAGGACAAGGACTTCTTTGAGAGAGAGCTTCAAAACGTTGAGGAGGAACAAAACAAGAGGGTTCGTGGATTCCGAGAGGTGATCGATTTGATTTCTGCTTCACAGAAACCTGTTGTCTCCCACAATTCCCTTAATGATTTCACATCTATTCATTCAAAATTTATCTCTCCCTTGCCCGGTAACATAGAGGAGTTCACAGGCTCCTTGAGCATGGTGTTTCCCCATATACTTGATGTCAAGCATCTGATGAAGAACATTGGCCCTTTAAGGAAGGTGACCAATATACCTGCAGCTATTTCCTACCTGAATAATCATTACTTTGCACCTATTGATTTGGAAACTTTGCATGAAGAAAATGAAGGCAAAAGTCATAGGCATAATGTTGTGAAGATATGTTATTTGTTTGCAAAGCTCTGCACCATTCTGAAAATTCCAGAGAATGCTAAGCCAATTGACTATCACCTATTAGCTCCCGCCACTGAAGAGTACACTAACATATCCAATCCGTTATCCAACATTCCCCAAGAATCAATCAACGAGGATATCCGAATCTGGAGAAGTAATATGAGAAAAATTAGCTGTAATCATTTGGCATTTGTGTGGGGATTTAGAAGTGGGTATACTGCTGGAATGCTCAAGAGCATGCTGCAAACGTCTCGTGACATTTTCTCTGAAGACTTTGATGTTCGGTTAGTAGATAAGAGCTGCGCCATTGTTGTTTTCCGCCAACCTGGGTTATCTGAAACTTTCCTTGATGTTGTGAGTAACGAAGACATTTGTGGATCTTTAAGGGAGATGGTCTCAGAGGGCCTAAGAGCAACGAGTTACCAAACTTACAAGAGAGTCTGTAACTTGGGCTTATGGGAGGCAGATTTAGCAGACTCCTTGGACAAAGCTTTAGAACATCCAGACTACAATATGGAAGCTGATTCAACAACAAATCGCAGAGAAATTCTTTGGTCCACCGACTCCATGATCAATTTCGATGACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0043167 GO:0043169
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

614

Amino Acids

69.22

Weight (kDa)

6.03

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 26 - 391 5.4e-64 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1141
AccB1I GGYRCC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 1466
AccII CGCG 2 cut(s) 95, 242
AclI AACGTT 1 cut(s) 882
AclWI GGATC 2 cut(s) 697, 1636
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 4 cut(s) 88, 1004, 1275, 1797
AcuI CTGAAG 3 cut(s) 371, 1347, 1539
AfaI GTAC 5 cut(s) 239, 458, 735, 811, 1334
AfiI CCNNNNNNNGG 4 cut(s) 512, 799, 800, 1022
AgsI TTSAA 8 cut(s) 115, 229, 381, 386, 627, 878, 1002, 1779
AjnI CCWGG 4 cut(s) 123, 255, 511, 1576
Alw26I GTCTC 4 cut(s) 688, 967, 1506, 1651
AlwI GGATC 2 cut(s) 697, 1636
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 3 cut(s) 126, 1105, 1654
ApeKI GCWGC 6 cut(s) 154, 196, 262, 1136, 1492, 1551
ApoI RAATTY 4 cut(s) 88, 1004, 1275, 1797
Asp700I GAANNNNTTC 1 cut(s) 777
AspLEI GCGC 2 cut(s) 41, 1556
AspS9I GGNCC 4 cut(s) 121, 1106, 1654, 1806
AsuC2I CCSGG 3 cut(s) 130, 191, 1023
AsuHPI GGTGA 6 cut(s) 79, 88, 298, 937, 1132, 1298
AvaII GGWCC 2 cut(s) 121, 1806
BanI GGYRCC 1 cut(s) 186
BarI GAAGNNNNNNTAC 4 cut(s) 221, 253, 1450, 1482
BauI CACGAG 3 cut(s) 34, 585, 1503
BbsI GAAGAC 2 cut(s) 1527, 1623
BbvI GCAGC 6 cut(s) 141, 183, 249, 1148, 1479, 1538
BccI CCATC 5 cut(s) 349, 373, 440, 664, 1636
BceAI ACGGC 4 cut(s) 32, 131, 184, 234
BcgI CGANNNNNNTGC 2 cut(s) 1484, 1518
BciT130I CCWGG 4 cut(s) 125, 257, 513, 1578
BclI TGATCA 1 cut(s) 1821
BcnI CCSGG 3 cut(s) 130, 191, 1023
BcoDI GTCTC 4 cut(s) 688, 967, 1506, 1651
BfaI CTAG 2 cut(s) 339, 621
BfmI CTRYAG 3 cut(s) 155, 263, 1134
BfoI RGCGCY 1 cut(s) 42
BfuAI ACCTGC 1 cut(s) 1141
BglI GCCNNNNNGGC 1 cut(s) 47
BlpI GCTNAGC 2 cut(s) 312, 1290
Bme1390I CCNGG 7 cut(s) 125, 130, 191, 257, 513, 1023, 1578
Bme18I GGWCC 2 cut(s) 121, 1806
BmgT120I GGNCC 4 cut(s) 121, 1106, 1654, 1806
BmiI GGNNCC 2 cut(s) 188, 1048
BmrFI CCNGG 7 cut(s) 125, 130, 191, 257, 513, 1023, 1578
BmsI GCATC 4 cut(s) 351, 628, 741, 1096
BpiI GAAGAC 2 cut(s) 1527, 1623
BplI GAGNNNNNCTC 2 cut(s) 1631, 1663
BpmI CTGGAG 2 cut(s) 767, 1420
Bpu1102I GCTNAGC 2 cut(s) 312, 1290
BpuEI CTTGAG 4 cut(s) 396, 676, 1072, 1466
BpuMI CCSGG 3 cut(s) 130, 191, 1023
Bsa29I ATCGAT 1 cut(s) 930
BsaAI YACGTR 1 cut(s) 609
BsaI GGTCTC 2 cut(s) 688, 1651
BsaJI CCNNGG 3 cut(s) 42, 1577, 1731
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 512, 799, 800, 1022
Bse1I ACTGG 1 cut(s) 750
BseBI CCWGG 4 cut(s) 125, 257, 513, 1578
BseCI ATCGAT 1 cut(s) 930
BseDI CCNNGG 3 cut(s) 42, 1577, 1731
BseGI GGATG 2 cut(s) 366, 1750
BseLI CCNNNNNNNGG 4 cut(s) 512, 799, 800, 1022
BseMII CTCAG 4 cut(s) 326, 638, 827, 1662
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 3 cut(s) 122, 902, 1049
BseXI GCAGC 6 cut(s) 141, 183, 249, 1148, 1479, 1538
BseYI CCCAGC 1 cut(s) 793
BsgI GTGCAG 2 cut(s) 16, 1246
Bsh1236I CGCG 2 cut(s) 95, 242
BshFI GGCC 3 cut(s) 128, 1107, 1656
BshNI GGYRCC 1 cut(s) 186
BshVI ATCGAT 1 cut(s) 930
BsiSI CCGG 3 cut(s) 129, 190, 1023
BslFI GGGAC 2 cut(s) 688, 776
BslI CCNNNNNNNGG 4 cut(s) 512, 799, 800, 1022
BsmAI GTCTC 4 cut(s) 688, 967, 1506, 1651
BsmBI CGTCTC 1 cut(s) 1506
BsmFI GGGAC 2 cut(s) 688, 776
BsmI GAATGC 2 cut(s) 1291, 1482
BsnI GGCC 3 cut(s) 128, 1107, 1656
Bso31I GGTCTC 2 cut(s) 688, 1651
Bsp143I GATC 4 cut(s) 689, 927, 1628, 1821
Bsp1720I GCTNAGC 2 cut(s) 312, 1290
BspANI GGCC 3 cut(s) 128, 1107, 1656
BspCNI CTCAG 4 cut(s) 325, 639, 826, 1661
BspDI ATCGAT 1 cut(s) 930
BspFNI CGCG 2 cut(s) 95, 242
BspLI GGNNCC 2 cut(s) 188, 1048
BspMAI CTGCAG 3 cut(s) 159, 267, 1138
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 2 cut(s) 697, 1636
BspT107I GGYRCC 1 cut(s) 186
BspTNI GGTCTC 2 cut(s) 688, 1651
BsrBI CCGCTC 1 cut(s) 311
BsrI ACTGG 1 cut(s) 750
BssECI CCNNGG 3 cut(s) 42, 1577, 1731
BssMI GATC 4 cut(s) 689, 927, 1628, 1821
BssNAI GTATAC 1 cut(s) 1467
BssSI CACGAG 3 cut(s) 34, 585, 1503
BssT1I CCWWGG 1 cut(s) 1731
Bst1107I GTATAC 1 cut(s) 1467
Bst2BI CACGAG 3 cut(s) 34, 585, 1503
Bst2UI CCWGG 4 cut(s) 125, 257, 513, 1578
Bst4CI ACNGT 1 cut(s) 550
Bst6I CTCTTC 1 cut(s) 1323
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 4 cut(s) 63, 277, 436, 1490
BstDEI CTNAG 6 cut(s) 312, 647, 813, 1290, 1648, 1658
BstEII GGTNACC 1 cut(s) 1120
BstF5I GGATG 2 cut(s) 366, 1750
BstFNI CGCG 2 cut(s) 95, 242
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 2 cut(s) 41, 1556
BstKTI GATC 4 cut(s) 692, 930, 1631, 1824
BstMAI GTCTC 4 cut(s) 688, 967, 1506, 1651
BstMBI GATC 4 cut(s) 689, 927, 1628, 1821
BstMWI GCNNNNNNNGC 8 cut(s) 25, 47, 92, 193, 239, 407, 1053, 1721
BstNI CCWGG 4 cut(s) 125, 257, 513, 1578
BstNSI RCATGY 1 cut(s) 1492
BstPI GGTNACC 1 cut(s) 1120
BstSCI CCNGG 7 cut(s) 123, 128, 189, 255, 511, 1021, 1576
BstSFI CTRYAG 3 cut(s) 155, 263, 1134
BstUI CGCG 2 cut(s) 95, 242
BstV1I GCAGC 6 cut(s) 141, 183, 249, 1148, 1479, 1538
BstV2I GAAGAC 2 cut(s) 1527, 1623
BstX2I RGATCY 1 cut(s) 1628
BstYI RGATCY 1 cut(s) 1628
BstZ17I GTATAC 1 cut(s) 1467
Bsu15I ATCGAT 1 cut(s) 930
BsuRI GGCC 3 cut(s) 128, 1107, 1656
BsuTUI ATCGAT 1 cut(s) 930
BtsCI GGATG 2 cut(s) 366, 1750
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 4 cut(s) 727, 743, 772, 1323
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 4 cut(s) 63, 277, 436, 1490
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 2 cut(s) 41, 1556
Cfr13I GGNCC 4 cut(s) 121, 1106, 1654, 1806
ClaI ATCGAT 1 cut(s) 930
CseI GACGC 1 cut(s) 716
Csp6I GTAC 5 cut(s) 238, 457, 734, 810, 1333
CviAII CATG 6 cut(s) 699, 703, 1057, 1193, 1489, 1819
CviQI GTAC 5 cut(s) 238, 457, 734, 810, 1333
DdeI CTNAG 6 cut(s) 312, 647, 813, 1290, 1648, 1658
DpnI GATC 4 cut(s) 691, 929, 1630, 1823
DpnII GATC 4 cut(s) 689, 927, 1628, 1821
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
EciI GGCGGA 2 cut(s) 260, 1559
Eco130I CCWWGG 1 cut(s) 1731
Eco31I GGTCTC 2 cut(s) 688, 1651
Eco32I GATATC 1 cut(s) 1390
Eco47I GGWCC 2 cut(s) 121, 1806
Eco57I CTGAAG 3 cut(s) 371, 1347, 1539
Eco91I GGTNACC 1 cut(s) 1120
EcoO109I RGGNCCY 2 cut(s) 121, 1654
EcoO65I GGTNACC 1 cut(s) 1120
EcoRII CCWGG 4 cut(s) 123, 255, 511, 1576
EcoRV GATATC 1 cut(s) 1390
EcoT14I CCWWGG 1 cut(s) 1731
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 1731
Esp3I CGTCTC 1 cut(s) 1506
FaeI CATG 6 cut(s) 702, 706, 1060, 1196, 1492, 1822
FalI AAGNNNNNCTT 4 cut(s) 723, 755, 845, 877
FaqI GGGAC 2 cut(s) 688, 776
FatI CATG 6 cut(s) 698, 702, 1056, 1192, 1488, 1818
FauI CCCGC 3 cut(s) 43, 172, 1327
FauNDI CATATG 1 cut(s) 396
FbaI TGATCA 1 cut(s) 1821
FblI GTMKAC 1 cut(s) 1466
FokI GGATG 2 cut(s) 373, 1737
FspBI CTAG 2 cut(s) 339, 621
GlaI GCGC 2 cut(s) 40, 1555
GsaI CCCAGC 1 cut(s) 797
GsuI CTGGAG 2 cut(s) 767, 1420
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 3 cut(s) 128, 1107, 1656
HapII CCGG 3 cut(s) 129, 190, 1023
HgaI GACGC 1 cut(s) 716
HhaI GCGC 2 cut(s) 41, 1556
Hin1II CATG 6 cut(s) 702, 706, 1060, 1196, 1492, 1822
Hin6I GCGC 2 cut(s) 39, 1554
HinP1I GCGC 2 cut(s) 39, 1554
HindIII AAGCTT 1 cut(s) 1740
HpaII CCGG 3 cut(s) 129, 190, 1023
HphI GGTGA 6 cut(s) 79, 88, 298, 937, 1132, 1298
Hpy166II GTNNAC 4 cut(s) 1041, 1335, 1467, 1809
Hpy188III TCNNGA 8 cut(s) 413, 585, 693, 1280, 1399, 1483, 1505, 1754
Hpy8I GTNNAC 4 cut(s) 1041, 1335, 1467, 1809
HpyAV CCTTC 5 cut(s) 76, 223, 410, 1111, 1199
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 4 cut(s) 389, 608, 882, 1499
HpyF10VI GCNNNNNNNGC 8 cut(s) 25, 47, 92, 193, 239, 407, 1053, 1721
HpyF3I CTNAG 6 cut(s) 312, 647, 813, 1290, 1648, 1658
HpySE526I ACGT 4 cut(s) 389, 608, 882, 1499
Hsp92II CATG 6 cut(s) 702, 706, 1060, 1196, 1492, 1822
HspAI GCGC 2 cut(s) 39, 1554
Ksp22I TGATCA 1 cut(s) 1821
Kzo9I GATC 4 cut(s) 689, 927, 1628, 1821
LmnI GCTCC 4 cut(s) 193, 247, 1052, 1321
Lsp1109I GCAGC 6 cut(s) 141, 183, 249, 1148, 1479, 1538
LweI GCATC 4 cut(s) 351, 628, 741, 1096
MaeI CTAG 2 cut(s) 339, 621
MaeII ACGT 4 cut(s) 389, 608, 882, 1499
MaeIII GTNAC 9 cut(s) 76, 390, 538, 1025, 1120, 1505, 1610, 1670, 1694
MalI GATC 4 cut(s) 691, 929, 1630, 1823
MbiI CCGCTC 1 cut(s) 311
MboI GATC 4 cut(s) 689, 927, 1628, 1821
MboII GAAGA 7 cut(s) 364, 1108, 1208, 1246, 1340, 1532, 1628
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 1 cut(s) 1628
MlyI GAGTC 3 cut(s) 1698, 1721, 1808
MmeI TCCRAC 3 cut(s) 114, 530, 1383
Mph1103I ATGCAT 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 777
MseI TTAA 5 cut(s) 429, 591, 978, 1112, 1634
MslI CAYNNNNRTG 1 cut(s) 1444
MspI CCGG 3 cut(s) 129, 190, 1023
MspR9I CCNGG 7 cut(s) 125, 130, 191, 257, 513, 1023, 1578
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 2 cut(s) 1291, 1482
MvaI CCWGG 4 cut(s) 125, 257, 513, 1578
MvnI CGCG 2 cut(s) 95, 242
MwoI GCNNNNNNNGC 8 cut(s) 25, 47, 92, 193, 239, 407, 1053, 1721
NciI CCSGG 3 cut(s) 130, 191, 1023
NdeI CATATG 1 cut(s) 396
NdeII GATC 4 cut(s) 689, 927, 1628, 1821
NlaIII CATG 6 cut(s) 702, 706, 1060, 1196, 1492, 1822
NlaIV GGNNCC 2 cut(s) 188, 1048
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 4 cut(s) 76, 390, 1120, 1505
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 1 cut(s) 1492
PaeI GCATGC 1 cut(s) 1492
PcsI WCGNNNNNNNCGW 1 cut(s) 916
PctI GAATGC 2 cut(s) 1291, 1482
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 7 cut(s) 476, 502, 530, 915, 1373, 1395, 1775
PflMI CCANNNNNTGG 1 cut(s) 512
PleI GAGTC 3 cut(s) 1697, 1721, 1808
PpsI GAGTC 3 cut(s) 1697, 1721, 1808
Ppu21I YACGTR 1 cut(s) 609
PpuMI RGGWCCY 1 cut(s) 121
Psp1406I AACGTT 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 4 cut(s) 123, 255, 511, 1576
PspEI GGTNACC 1 cut(s) 1120
PspFI CCCAGC 1 cut(s) 793
PspGI CCWGG 4 cut(s) 123, 255, 511, 1576
PspN4I GGNNCC 2 cut(s) 188, 1048
PspPI GGNCC 4 cut(s) 121, 1106, 1654, 1806
PspPPI RGGWCCY 1 cut(s) 121
PstI CTGCAG 3 cut(s) 159, 267, 1138
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 1628
RsaI GTAC 5 cut(s) 239, 458, 735, 811, 1334
RsaNI GTAC 5 cut(s) 238, 457, 734, 810, 1333
RseI CAYNNNNRTG 1 cut(s) 1444
SaqAI TTAA 5 cut(s) 429, 591, 978, 1112, 1634
Sau3AI GATC 4 cut(s) 689, 927, 1628, 1821
Sau96I GGNCC 4 cut(s) 121, 1106, 1654, 1806
SchI GAGTC 3 cut(s) 1698, 1721, 1808
ScrFI CCNGG 7 cut(s) 125, 130, 191, 257, 513, 1023, 1578
SfaNI GCATC 4 cut(s) 351, 628, 741, 1096
SfcI CTRYAG 3 cut(s) 155, 263, 1134
SinI GGWCC 2 cut(s) 121, 1806
SmiMI CAYNNNNRTG 1 cut(s) 1444
SmlI CTYRAG 4 cut(s) 411, 655, 1051, 1481
SmoI CTYRAG 4 cut(s) 411, 655, 1051, 1481
SphI GCATGC 1 cut(s) 1492
SspMI CTAG 2 cut(s) 339, 621
StyD4I CCNGG 7 cut(s) 123, 128, 189, 255, 511, 1021, 1576
StyI CCWWGG 1 cut(s) 1731
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 4 cut(s) 392, 611, 885, 1502
TaqI TCGA 3 cut(s) 692, 930, 1830
TatI WGTACW 2 cut(s) 733, 1332
TauI GCSGC 6 cut(s) 8, 22, 50, 245, 275, 485
TfiI GAWTC 7 cut(s) 476, 502, 530, 915, 1373, 1395, 1775
Tru1I TTAA 5 cut(s) 429, 591, 978, 1112, 1634
Tru9I TTAA 5 cut(s) 429, 591, 978, 1112, 1634
TscAI CASTG 4 cut(s) 727, 750, 772, 1330
TseFI GTSAC 4 cut(s) 76, 390, 1120, 1505
TseI GCWGC 6 cut(s) 154, 196, 262, 1136, 1492, 1551
Tsp45I GTSAC 4 cut(s) 76, 390, 1120, 1505
TspDTI ATGAA 7 cut(s) 360, 719, 786, 986, 1109, 1209, 1218
TspGWI ACGGA 1 cut(s) 1341
TspRI CASTG 4 cut(s) 727, 750, 772, 1330
Van91I CCANNNNNTGG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 121, 1806
XapI RAATTY 4 cut(s) 88, 1004, 1275, 1797
XceI RCATGY 1 cut(s) 1492
XmiI GTMKAC 1 cut(s) 1466
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 2 cut(s) 339, 621
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.