AT3G46450

SEC14 cytosolic factor family protein phosphoglyceride transfer family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011426
Physical Location & Seq
Reverse (-)
17092848 .. 17095729
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G46450.3

Sequence Viewer

Length: 1461 bp
ATGGGGGATAATTCTGGTAGAAGTAGAAACTCTATGATGGCAACTGTTTCTTCTGGGAAAGAGTTGTTACCTAAAAGAAGCTTAGTAGCTGCTATTCCAAGAGCTGTTCAGCAGACAATCTCTAAGCATGTTTCTTTTGGGAGTAATGGTAGATTTGGAAGTGGAACCGCTCGTAATGCAGCTGTGTTTCTGCTTAAAATCGCTGTTTTAGAAGTTGTTCGAAGGGTTTCAAAGGCTAAATGTCCTCATTTGTGGAATAGTCTTCAGGCGTTGCAATGTCTTTGTTATCCTCCTCTTAAGTGGATTCAAAGATGGGCTCCTTTCAAGGAGTTGATCAATGCAATGCAGATGTTGTCTCGGCCATTGTTGGTCATCACGATAGCTGAGGCTCTGACAGACCAGTCAGAGTTAAAGCAAGAAGCCTCAGGTGGTACCAATTCTCATGCATCTTCAGAATCAGAATCACAATCTGACTCGCAGACTTTACAGTCTCCATCAGATATAAGAATTGAAGATGAGGCTCCACTTCCTGTAATTTCACAAGACTGGCTTAGAAAACTCTATGAAGAACTGGAAAAGCAGAGACTTAGCTTACCAGAGAGACTCAATGAAGACGAGCTTCATAGATTCTACAGGGTGTCAAATGGAGACTTCACATCCTTACTATCTTCGATAAAAAAGACAATTCATTGGAGGGAGACTTACAGAATTCTTTCTGAAGAAGAACTTGAGACATGGTCAAGTTTACTCTTTTGGCATGGATATGATAAGAACCAACGACCTTGCCTCATTGTTCGTTTGGGGCTAGCTTTTCTAAAGTTGCCATCTCATGAGAGACCTCGTTTCGCTCAGGCAATCATATCTCAGGTAGAGCACGGCGTTTTGCATCTTCTGACCCCTGAGAATTCAGAACTTACAGTGTTAGTCGATTGTGAAGGATTATCTCCTCTGAGGATTCCCATGCAGATGATGAGATCTTGTTCTTCCATTCTTCAAGATCACTTCCCTAACCGTCTTGGCTGCCTATTCATCATTCGCCTCCCTCCTGTTGTCCGTGTCATTTCCCAGACTTTCATCCAAATTCTTAGACCAACCACACGCAAGAAGCTGAGAATTGAAGGGGAAACTTTCCATCGAGTCCTCTCTGAATACCTCCAGACACTTCCTTCATACCTTGGTAGCAACTGCAACTGCAAAAGATGCTCAAATCTCAACGAACAAGATCCACCACAGCCACAAACACACCCAAGAAGAAAGAGAAGAAGCTCGAGTGAAACAGAGAAGCTAGACGACAGCCACTGGAGTTACAATGCCCAGACACCTGATTTATCCTATGAAGACGAGCCGAGCCTGAACATATGCAGCCAAGTTCTGCGAACAGCAGTCGTGTTCTTACTCATGATATGGCTCTTCGGTGCACTACTAGCTGGATTTGCTGATCCTGAGAGCAGACCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

486

Amino Acids

55.39

Weight (kDa)

8.7

Isoelectric Point (pI)

61.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 248 - 387 8.8e-26 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 400, 487
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 431
AccBSI CCGCTC 1 cut(s) 170
AciI CCGC 1 cut(s) 168
AclWI GGATC 2 cut(s) 1217, 1433
AcoI YGGCCR 1 cut(s) 359
AcsI RAATTY 3 cut(s) 708, 904, 1080
AcuI CTGAAG 3 cut(s) 248, 435, 738
AfaI GTAC 1 cut(s) 433
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 6 cut(s) 231, 308, 325, 512, 995, 1118
Alw21I GWGCWC 2 cut(s) 876, 1420
Alw26I GTCTC 8 cut(s) 360, 495, 577, 595, 642, 692, 725, 829
Alw44I GTGCAC 1 cut(s) 1416
AlwI GGATC 2 cut(s) 1217, 1433
AlwNI CAGNNNCTG 1 cut(s) 1299
Ama87I CYCGRG 1 cut(s) 1267
AoxI GGCC 1 cut(s) 359
ApaLI GTGCAC 1 cut(s) 1416
ApeKI GCWGC 4 cut(s) 89, 179, 1020, 1362
ApoI RAATTY 3 cut(s) 708, 904, 1080
Asp700I GAANNNNTTC 3 cut(s) 216, 226, 712
Asp718I GGTACC 1 cut(s) 431
AsuII TTCGAA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 805
AvaI CYCGRG 1 cut(s) 1267
AxyI CCTNAGG 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 1420
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 319
BbsI GAAGAC 3 cut(s) 254, 618, 1344
Bbv12I GWGCWC 2 cut(s) 876, 1420
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 4 cut(s) 76, 191, 1007, 1374
BccI CCATC 5 cut(s) 31, 306, 502, 832, 1140
BceAI ACGGC 1 cut(s) 892
BclI TGATCA 1 cut(s) 333
BcoDI GTCTC 8 cut(s) 360, 495, 577, 595, 642, 692, 725, 829
BfaI CTAG 3 cut(s) 806, 1286, 1424
BfmI CTRYAG 1 cut(s) 631
BfrI CTTAAG 1 cut(s) 296
BglII AGATCT 1 cut(s) 974
BisI GCNGC 4 cut(s) 90, 180, 1021, 1363
BlsI GCNGC 4 cut(s) 91, 181, 1022, 1364
BmeT110I CYCGRG 1 cut(s) 1267
BmiI GGNNCC 4 cut(s) 166, 318, 433, 522
BmsI GCATC 3 cut(s) 455, 895, 1190
BmtI GCTAGC 1 cut(s) 809
BpiI GAAGAC 3 cut(s) 254, 618, 1344
BpmI CTGGAG 2 cut(s) 1139, 1321
Bpu10I CCTNAGC 2 cut(s) 384, 849
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 749
BsaI GGTCTC 1 cut(s) 829
BsaJI CCNNGG 1 cut(s) 1174
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bse1I ACTGG 4 cut(s) 400, 551, 576, 1304
Bse21I CCTNAGG 1 cut(s) 424
Bse3DI GCAATG 2 cut(s) 281, 348
BseDI CCNNGG 1 cut(s) 1174
BseGI GGATG 2 cut(s) 656, 1074
BseMI GCAATG 2 cut(s) 281, 348
BseMII CTCAG 8 cut(s) 375, 438, 863, 878, 891, 941, 1100, 1434
BseNI ACTGG 4 cut(s) 400, 551, 576, 1304
BseRI GAGGAG 2 cut(s) 282, 936
BseSI GKGCMC 1 cut(s) 1420
BseXI GCAGC 4 cut(s) 76, 191, 1007, 1374
BshFI GGCC 1 cut(s) 361
BshNI GGYRCC 1 cut(s) 431
BsiHKAI GWGCWC 2 cut(s) 876, 1420
BsiHKCI CYCGRG 1 cut(s) 1267
BsmAI GTCTC 8 cut(s) 360, 495, 577, 595, 642, 692, 725, 829
BsnI GGCC 1 cut(s) 361
Bso31I GGTCTC 1 cut(s) 829
BsoBI CYCGRG 1 cut(s) 1267
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 3 cut(s) 319, 876, 1420
Bsp143I GATC 5 cut(s) 333, 974, 997, 1222, 1438
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 8 cut(s) 376, 437, 862, 877, 892, 942, 1101, 1435
BspHI TCATGA 2 cut(s) 829, 1398
BspLI GGNNCC 4 cut(s) 166, 318, 433, 522
BspOI GCTAGC 1 cut(s) 809
BspPI GGATC 2 cut(s) 1217, 1433
BspQI GCTCTTC 1 cut(s) 1415
BspT104I TTCGAA 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 431
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 829
BsrBI CCGCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 281, 348
BsrI ACTGG 4 cut(s) 400, 551, 576, 1304
BssECI CCNNGG 1 cut(s) 1174
BssMI GATC 5 cut(s) 333, 974, 997, 1222, 1438
BssT1I CCWWGG 1 cut(s) 1174
Bst4CI ACNGT 4 cut(s) 46, 489, 919, 1013
Bst6I CTCTTC 1 cut(s) 1415
BstAFI CTTAAG 1 cut(s) 296
BstAPI GCANNNNNTGC 1 cut(s) 1200
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 807
BstF5I GGATG 2 cut(s) 656, 1074
BstKTI GATC 5 cut(s) 336, 977, 1000, 1225, 1441
BstMAI GTCTC 8 cut(s) 360, 495, 577, 595, 642, 692, 725, 829
BstMBI GATC 5 cut(s) 333, 974, 997, 1222, 1438
BstMWI GCNNNNNNNGC 4 cut(s) 176, 1200, 1424, 1433
BstNSI RCATGY 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 631
BstSLI GKGCMC 1 cut(s) 1420
BstV1I GCAGC 4 cut(s) 76, 191, 1007, 1374
BstV2I GAAGAC 3 cut(s) 254, 618, 1344
BstX2I RGATCY 2 cut(s) 974, 1222
BstYI RGATCY 2 cut(s) 974, 1222
Bsu36I CCTNAGG 1 cut(s) 424
BsuRI GGCC 1 cut(s) 361
BtsCI GGATG 2 cut(s) 656, 1074
BtsIMutI CAGTG 2 cut(s) 924, 1297
Cac8I GCNNGC 1 cut(s) 807
CaiI CAGNNNCTG 1 cut(s) 1299
CciI TCATGA 2 cut(s) 829, 1398
Csp6I GTAC 1 cut(s) 432
CviAII CATG 7 cut(s) 128, 443, 735, 758, 830, 961, 1399
CviQI GTAC 1 cut(s) 432
DpnI GATC 5 cut(s) 335, 976, 999, 1224, 1440
DpnII GATC 5 cut(s) 333, 974, 997, 1222, 1438
DrdI GACNNNNNNGTC 2 cut(s) 400, 487
DseDI GACNNNNNNGTC 2 cut(s) 400, 487
EaeI YGGCCR 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 1415
EarI CTCTTC 1 cut(s) 1415
Eco130I CCWWGG 1 cut(s) 1174
Eco24I GRGCYC 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 829
Eco57I CTGAAG 3 cut(s) 248, 435, 738
Eco81I CCTNAGG 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 1267
EcoRI GAATTC 2 cut(s) 708, 904
EcoT14I CCWWGG 1 cut(s) 1174
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 1174
FaeI CATG 7 cut(s) 131, 446, 738, 761, 833, 964, 1402
FalI AAGNNNNNCTT 6 cut(s) 534, 566, 603, 635, 711, 743
FatI CATG 7 cut(s) 127, 442, 734, 757, 829, 960, 1398
FauNDI CATATG 1 cut(s) 1358
FbaI TGATCA 1 cut(s) 333
Fnu4HI GCNGC 4 cut(s) 90, 180, 1021, 1363
FokI GGATG 2 cut(s) 643, 1061
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 4 cut(s) 90, 180, 1021, 1363
FspBI CTAG 3 cut(s) 806, 1286, 1424
GluI GCNGC 4 cut(s) 90, 180, 1021, 1363
GsuI CTGGAG 2 cut(s) 1139, 1321
HaeIII GGCC 1 cut(s) 361
Hin1II CATG 7 cut(s) 131, 446, 738, 761, 833, 964, 1402
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 8 cut(s) 304, 455, 461, 473, 603, 627, 955, 1137
Hpy166II GTNNAC 2 cut(s) 746, 1418
Hpy188III TCNNGA 6 cut(s) 376, 830, 995, 1156, 1399, 1442
Hpy8I GTNNAC 2 cut(s) 746, 1418
HpyAV CCTTC 4 cut(s) 216, 929, 1112, 1176
HpyCH4III ACNGT 4 cut(s) 46, 489, 919, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 1200, 1424, 1433
Hsp92II CATG 7 cut(s) 131, 446, 738, 761, 833, 964, 1402
KpnI GGTACC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 333
Kzo9I GATC 5 cut(s) 333, 974, 997, 1222, 1438
LguI GCTCTTC 1 cut(s) 1415
LmnI GCTCC 2 cut(s) 322, 526
Lsp1109I GCAGC 4 cut(s) 76, 191, 1007, 1374
LweI GCATC 3 cut(s) 455, 895, 1190
MaeI CTAG 3 cut(s) 806, 1286, 1424
MaeIII GTNAC 2 cut(s) 66, 1304
MalI GATC 5 cut(s) 335, 976, 999, 1224, 1440
MbiI CCGCTC 1 cut(s) 170
MboI GATC 5 cut(s) 333, 974, 997, 1222, 1438
MflI RGATCY 2 cut(s) 974, 1222
MhlI GDGCHC 3 cut(s) 319, 876, 1420
MluCI AATT 9 cut(s) 10, 436, 507, 534, 684, 708, 904, 1080, 1113
MlyI GAGTC 3 cut(s) 467, 597, 1146
Mph1103I ATGCAT 1 cut(s) 448
MroXI GAANNNNTTC 3 cut(s) 216, 226, 712
MseI TTAA 4 cut(s) 195, 297, 410, 1459
MslI CAYNNNNRTG 2 cut(s) 762, 965
MspA1I CMGCKG 1 cut(s) 182
MspCI CTTAAG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 176, 1200, 1424, 1433
NdeI CATATG 1 cut(s) 1358
NdeII GATC 5 cut(s) 333, 974, 997, 1222, 1438
NheI GCTAGC 1 cut(s) 805
NlaIII CATG 7 cut(s) 131, 446, 738, 761, 833, 964, 1402
NlaIV GGNNCC 4 cut(s) 166, 318, 433, 522
NmeAIII GCCGAG 2 cut(s) 337, 1371
NsiI ATGCAT 1 cut(s) 448
NspI RCATGY 1 cut(s) 131
NspV TTCGAA 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 1267
PagI TCATGA 2 cut(s) 829, 1398
PciSI GCTCTTC 1 cut(s) 1415
PdmI GAANNNNTTC 3 cut(s) 216, 226, 712
PfeI GAWTC 5 cut(s) 304, 455, 461, 627, 955
PflFI GACNNNGTC 1 cut(s) 736
PkrI GCNGC 4 cut(s) 91, 181, 1022, 1364
PleI GAGTC 3 cut(s) 467, 597, 1145
PpsI GAGTC 3 cut(s) 467, 597, 1145
PspN4I GGNNCC 4 cut(s) 166, 318, 433, 522
PspXI VCTCGAGB 1 cut(s) 1267
PstNI CAGNNNCTG 1 cut(s) 1299
PsuI RGATCY 2 cut(s) 974, 1222
PsyI GACNNNGTC 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 2 cut(s) 762, 965
SapI GCTCTTC 1 cut(s) 1415
SaqAI TTAA 4 cut(s) 195, 297, 410, 1459
SatI GCNGC 4 cut(s) 90, 180, 1021, 1363
Sau3AI GATC 5 cut(s) 333, 974, 997, 1222, 1438
SchI GAGTC 3 cut(s) 467, 597, 1146
SduI GDGCHC 3 cut(s) 319, 876, 1420
SfaNI GCATC 3 cut(s) 455, 895, 1190
SfcI CTRYAG 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 1267
SfuI TTCGAA 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 1267
SmiMI CAYNNNNRTG 2 cut(s) 762, 965
SmlI CTYRAG 3 cut(s) 296, 728, 1267
SmoI CTYRAG 3 cut(s) 296, 728, 1267
Sse9I AATT 9 cut(s) 10, 436, 507, 534, 684, 708, 904, 1080, 1113
SsiI CCGC 1 cut(s) 168
SspMI CTAG 3 cut(s) 806, 1286, 1424
StyI CCWWGG 1 cut(s) 1174
TaaI ACNGT 4 cut(s) 46, 489, 919, 1013
TaqI TCGA 5 cut(s) 220, 671, 927, 1135, 1268
TasI AATT 9 cut(s) 10, 436, 507, 534, 684, 708, 904, 1080, 1113
TfiI GAWTC 5 cut(s) 304, 455, 461, 627, 955
Tru1I TTAA 4 cut(s) 195, 297, 410, 1459
Tru9I TTAA 4 cut(s) 195, 297, 410, 1459
TscAI CASTG 2 cut(s) 924, 1304
TseI GCWGC 4 cut(s) 89, 179, 1020, 1362
TspDTI ATGAA 8 cut(s) 579, 611, 624, 677, 1018, 1063, 1158, 1350
TspGWI ACGGA 1 cut(s) 1043
TspRI CASTG 2 cut(s) 924, 1304
Tth111I GACNNNGTC 1 cut(s) 736
Vha464I CTTAAG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 1416
XapI RAATTY 3 cut(s) 708, 904, 1080
XceI RCATGY 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 1267
XmnI GAANNNNTTC 3 cut(s) 216, 226, 712
XspI CTAG 3 cut(s) 806, 1286, 1424
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.