Rh5AG418400

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011426
Physical Location & Seq
Reverse (-)
72008548 .. 72015317
6770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG418400.1

Sequence Viewer

Length: 1131 bp
ATGGCCAATATAGTTACATTCTTCCAGACGGTATCGAAGCCATTACTAGCCCTCTCCATTGCAACAGCTTTTACTGATGAATTAGAATTCGGTCCTAGAACTTCAGATGATGTCAACAATTCTGATGTGCGTCCAGAAGTACACCCAGAAGTATCCTCTGCAGAGTCTGCTTTGGATACAAGTGTTTCTGAAGAAGTGCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTTCATAACGAGCTCGAAAGACAAGGGCTTAGCTTGCCAGAAAGAATTGATGAGGAGGAACTTCGCAGGTTCTACACAGCTGCTAACAGAGACTTCTCAAGCTTTCTGTCTGCAATAAAGAAGACGATCAGGTGGAGAGAGACTTATGGAATTCTTTCGGTACAGGAGCTTGAGAAATGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTCGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACCTGTTCTTCGCTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACTTTCATCCAAGTTTTGAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAAGATGTACCGGAAGATTCTCTCTGAGTGCCTAGAGACACTCCCTTCATGTCTTGGTGGCAAATGCATGTGTAGAATATGTTCAGAAATTAACACGAAAGATATGCAGCAGCCTCGCACAAATGATATCATGAAGAGAGAGCTGAAAGTGAGTGTTTGCGAGGATGGGAGTTTATGCTCTCCCGGTCCAACTTGTGAGACCGAGGTTGAACCGAACCATTACTGTGATCAGCTGCTGAGAACTGCCATAGTGACCATCCTTATGTTTTGGATTTTAGTAGCTTTTATCGCTGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

376

Amino Acids

42.56

Weight (kDa)

5.78

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 139 - 286 2.7e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 86, 384, 630
AcuI CTGAAG 2 cut(s) 87, 210
AfaI GTAC 5 cut(s) 141, 396, 471, 711, 806
AgsI TTSAA 4 cut(s) 628, 763, 794, 1007
AjnI CCWGG 1 cut(s) 1102
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 4 cut(s) 318, 368, 827, 989
AlwNI CAGNNNCTG 2 cut(s) 167, 1033
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 314, 904, 907, 1030
ApoI RAATTY 3 cut(s) 86, 384, 630
Asp700I GAANNNNTTC 3 cut(s) 388, 655, 877
AspS9I GGNCC 2 cut(s) 92, 983
AsuC2I CCSGG 1 cut(s) 981
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 2 cut(s) 92, 983
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 301, 916, 919, 1017
BccI CCATC 4 cut(s) 216, 508, 956, 1061
BceAI ACGGC 2 cut(s) 488, 728
BcgI CGANNNNNNTGC 4 cut(s) 883, 893, 917, 927
BciT130I CCWGG 1 cut(s) 1104
BciVI GTATCC 2 cut(s) 163, 169
BclI TGATCA 2 cut(s) 547, 1024
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 4 cut(s) 318, 368, 827, 989
BfaI CTAG 4 cut(s) 47, 96, 482, 830
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 291
BfuI GTATCC 2 cut(s) 163, 169
BisI GCNGC 4 cut(s) 315, 905, 908, 1031
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 4 cut(s) 316, 906, 909, 1032
Bme1390I CCNGG 2 cut(s) 981, 1104
Bme18I GGWCC 2 cut(s) 92, 983
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 2 cut(s) 92, 983
BmrFI CCNGG 2 cut(s) 981, 1104
BmsI GCATC 3 cut(s) 221, 498, 562
BpiI GAAGAC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 4 cut(s) 316, 425, 510, 525
BpuMI CCSGG 1 cut(s) 981
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 2 cut(s) 999, 1102
BsaWI WCCGGW 1 cut(s) 807
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 1 cut(s) 1104
BseDI CCNNGG 2 cut(s) 999, 1102
BseGI GGATG 3 cut(s) 750, 967, 1053
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 813, 1025
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 4 cut(s) 301, 916, 919, 1017
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 2 cut(s) 808, 981
BslFI GGGAC 1 cut(s) 1098
BsmAI GTCTC 4 cut(s) 318, 368, 827, 989
BsmFI GGGAC 1 cut(s) 1098
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 4 cut(s) 360, 547, 673, 1024
Bsp1720I GCTNAGC 1 cut(s) 263
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 814, 1026
BspHI TCATGA 2 cut(s) 927, 1127
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 989
BsrDI GCAATG 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 999, 1102
BssMI GATC 4 cut(s) 360, 547, 673, 1024
Bst2UI CCWGG 1 cut(s) 1104
Bst4CI ACNGT 4 cut(s) 31, 595, 689, 1022
Bst6I CTCTTC 1 cut(s) 926
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 269, 487, 497
BstDEI CTNAG 3 cut(s) 263, 822, 1034
BstF5I GGATG 3 cut(s) 750, 967, 1053
BstKTI GATC 4 cut(s) 363, 550, 676, 1027
BstMAI GTCTC 4 cut(s) 318, 368, 827, 989
BstMBI GATC 4 cut(s) 360, 547, 673, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 167, 268, 1085
BstNI CCWGG 1 cut(s) 1104
BstNSI RCATGY 1 cut(s) 868
BstSCI CCNGG 2 cut(s) 979, 1102
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 4 cut(s) 301, 916, 919, 1017
BstV2I GAAGAC 1 cut(s) 362
BsuI GTATCC 2 cut(s) 163, 169
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 750, 967, 1053
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 269, 487, 497
CaiI CAGNNNCTG 2 cut(s) 167, 1033
CciI TCATGA 2 cut(s) 927, 1127
Cfr13I GGNCC 2 cut(s) 92, 983
CseI GACGC 1 cut(s) 119
Csp6I GTAC 5 cut(s) 140, 395, 470, 710, 805
CviAII CATG 7 cut(s) 434, 551, 637, 846, 865, 928, 1128
CviQI GTAC 5 cut(s) 140, 395, 470, 710, 805
DdeI CTNAG 3 cut(s) 263, 822, 1034
DpnI GATC 4 cut(s) 362, 549, 675, 1026
DpnII GATC 4 cut(s) 360, 547, 673, 1024
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 926
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 92, 983
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 87, 210
Eco88I CYCGRG 1 cut(s) 774
EcoICRI GAGCTC 1 cut(s) 247
EcoRI GAATTC 2 cut(s) 86, 384
EcoRII CCWGG 1 cut(s) 1102
EcoRV GATATC 1 cut(s) 925
EcoT22I ATGCAT 1 cut(s) 866
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 7 cut(s) 437, 554, 640, 849, 868, 931, 1131
FaqI GGGAC 1 cut(s) 1098
FatI CATG 7 cut(s) 433, 550, 636, 845, 864, 927, 1127
FbaI TGATCA 2 cut(s) 547, 1024
Fnu4HI GCNGC 4 cut(s) 315, 905, 908, 1031
FokI GGATG 3 cut(s) 737, 974, 1040
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 315, 905, 908, 1031
FspBI CTAG 4 cut(s) 47, 96, 482, 830
GluI GCNGC 4 cut(s) 315, 905, 908, 1031
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 808, 981
HgaI GACGC 1 cut(s) 119
Hin1II CATG 7 cut(s) 437, 554, 640, 849, 868, 931, 1131
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 3 cut(s) 164, 555, 814
HpaII CCGG 2 cut(s) 808, 981
Hpy166II GTNNAC 2 cut(s) 115, 142
Hpy188I TCNGA 6 cut(s) 106, 124, 190, 217, 823, 883
Hpy188III TCNNGA 5 cut(s) 25, 134, 506, 928, 1128
Hpy8I GTNNAC 2 cut(s) 115, 142
HpyAV CCTTC 3 cut(s) 605, 788, 852
HpyCH4III ACNGT 4 cut(s) 31, 595, 689, 1022
HpyCH4V TGCA 8 cut(s) 62, 161, 347, 489, 640, 670, 864, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 268, 1085
HpyF3I CTNAG 3 cut(s) 263, 822, 1034
Hsp92II CATG 7 cut(s) 437, 554, 640, 849, 868, 931, 1131
Ksp22I TGATCA 2 cut(s) 547, 1024
Kzo9I GATC 4 cut(s) 360, 547, 673, 1024
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 4 cut(s) 301, 916, 919, 1017
LweI GCATC 3 cut(s) 221, 498, 562
MaeI CTAG 4 cut(s) 47, 96, 482, 830
MaeIII GTNAC 2 cut(s) 13, 1048
MalI GATC 4 cut(s) 362, 549, 675, 1026
MboI GATC 4 cut(s) 360, 547, 673, 1024
MboII GAAGA 9 cut(s) 13, 203, 367, 651, 811, 823, 943, 1113, 1116
MhlI GDGCHC 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 173, 564
MmeI TCCRAC 1 cut(s) 1010
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 866
MroXI GAANNNNTTC 3 cut(s) 388, 655, 877
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 888
MslI CAYNNNNRTG 2 cut(s) 1020, 1058
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 314, 1030
MspI CCGG 2 cut(s) 808, 981
MspR9I CCNGG 2 cut(s) 981, 1104
MvaI CCWGG 1 cut(s) 1104
MwoI GCNNNNNNNGC 3 cut(s) 167, 268, 1085
NciI CCSGG 1 cut(s) 981
NdeII GATC 4 cut(s) 360, 547, 673, 1024
NlaIII CATG 7 cut(s) 437, 554, 640, 849, 868, 931, 1131
NmuCI GTSAC 1 cut(s) 1048
NsiI ATGCAT 1 cut(s) 866
NspI RCATGY 1 cut(s) 868
PagI TCATGA 2 cut(s) 927, 1127
PdmI GAANNNNTTC 3 cut(s) 388, 655, 877
PfeI GAWTC 1 cut(s) 814
PkrI GCNGC 4 cut(s) 316, 906, 909, 1032
PleI GAGTC 2 cut(s) 172, 563
PpsI GAGTC 2 cut(s) 172, 563
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 1102
PspGI CCWGG 1 cut(s) 1102
PspPI GGNCC 2 cut(s) 92, 983
PsrI GAACNNNNNNTAC 2 cut(s) 862, 894
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 2 cut(s) 167, 1033
PvuII CAGCTG 2 cut(s) 314, 1030
RsaI GTAC 5 cut(s) 141, 396, 471, 711, 806
RsaNI GTAC 5 cut(s) 140, 395, 470, 710, 805
RseI CAYNNNNRTG 2 cut(s) 1020, 1058
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 888
SatI GCNGC 4 cut(s) 315, 905, 908, 1031
Sau3AI GATC 4 cut(s) 360, 547, 673, 1024
Sau96I GGNCC 2 cut(s) 92, 983
SchI GAGTC 2 cut(s) 173, 564
ScrFI CCNGG 2 cut(s) 981, 1104
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 3 cut(s) 221, 498, 562
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 2 cut(s) 92, 983
SmiMI CAYNNNNRTG 2 cut(s) 1020, 1058
SmlI CTYRAG 4 cut(s) 331, 404, 525, 540
SmoI CTYRAG 4 cut(s) 331, 404, 525, 540
SspMI CTAG 4 cut(s) 47, 96, 482, 830
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 979, 1102
TaaI ACNGT 4 cut(s) 31, 595, 689, 1022
TaqI TCGA 2 cut(s) 35, 249
TaqII GACCGA 2 cut(s) 80, 1013
TatI WGTACW 1 cut(s) 139
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 1 cut(s) 888
Tru9I TTAA 1 cut(s) 888
TseFI GTSAC 1 cut(s) 1048
TseI GCWGC 4 cut(s) 314, 904, 907, 1030
Tsp45I GTSAC 1 cut(s) 1048
TspDTI ATGAA 7 cut(s) 93, 227, 551, 739, 834, 944, 1116
VpaK11BI GGWCC 2 cut(s) 92, 983
XapI RAATTY 3 cut(s) 86, 384, 630
XceI RCATGY 1 cut(s) 868
XmnI GAANNNNTTC 3 cut(s) 388, 655, 877
XspI CTAG 4 cut(s) 47, 96, 482, 830
Zsp2I ATGCAT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.