MD03G1096600.v1.1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011426
Physical Location & Seq
Forward (+)
8312090 .. 8317202
5113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1096600.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGAATGGAAAGACGAAGTCGCAGAATCATATAGTTGCTTCTCGCCCCAAGGGCTCTCTGCAGGATGTAGGGTCGCTTCGAATTGGGAGTGGCGTGGTGAGCCAGGCAGCTTTGTTTCTGCTCAAAATGGCTGCATTGGAGACAGTAAGGCGGTTCTCAAGGGCCAAGTGCCCGTGGGCATGGCGTGGCTTCCAGGCTTTGCAATTTCTGTGTTACCCTCCGTTCAAGTGGTTTCGAAGATTTGCGCCCTTCAAGGCTTTGGTCAATGGCATGCAGGTAGTTTCAAAACCATTACTAGTGCTTTCCATTGCAACAGTGTTCTCTGATGAAGCGGAGTTCTTTAATAGAACCTCAGATGGCATTAACAATTCTGATGCATGTTCAGAAGTGCAGTCGGAAGTATTGTCTGAGTGCATTAACGATTCTACTTTGGATACAAGGGTTCCTGATCAATTTCCTCAAAATGTAGAACCTGAAATGTGGTTGGTAGAACTCCATGAAGAGCTTGAAAGACAAGGGATTAGCTTGCCAGAAAGAATTGATGAGGAGGAACTTCGTAGGTTTTACACTGCTGCAAATGGAGACTTTTCTCGCTTGCTATCATCAGTAAAGAAGACAATCCATTGGAGGGAGACTTACGGAATTCTTTCAATACAGGAGCTTGAGATGTGGTCAAATATGGTTTTCTGGCATGGATTTGATGTTAAGCACCGACCTTGCCTCATAGTGCGACTTGGGCTAGCTTGCACTAGCTTGCTATCTCATGATAGACCTCGCTTTGCTCAAGCTATCATTTCCCAAGTAGAGCATGGAGTCTATCATTTGCTTGATGCCACCAATGCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTATCTCCATTGAAAATTCCCATGCAAGTTATGAGGACTTGTTCTTCTCTTTTGCAAGATCACTTCCCCAACCGTCTTGGCTGTCTGTTTGTTATTCGGCTTCCTCCAATGCTTCGTGTTATTGCTCAAACCTTTATTCAAGTTTTGAAACCTTATACCCGAGAGAAGTTGAGAATTGAAGGTGAGAGTTACCGGAAGATTCTCTCTGAGTACCTTGAGGCACTTCCATTATATCTTGGTGGCAAATGCCAGTGCAAAATATGTACAGATATGTGGCACCCTCGCACAAATGAAGTTATCAAGACAGAGCTGAGAGAAGATTTGCACGAGGGTCGGAATCCACTTCCAGCCCATCCAACTTATGAGAATGAGGTTGACATGGAAGATAACTGGGATCAGTTGGTGAGGACTGCCATATTTGGTCTCGTTATGGTATGGGTTTTCATAGCATTCCTTTCTTTATTCTTTGACCCCGAAAGCCGTCCCTTTCCCTTTTCTCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

460

Amino Acids

52.76

Weight (kDa)

6.48

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 225 - 372 2.2e-26 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 1149
AciI CCGC 2 cut(s) 151, 332
AclWI GGATC 1 cut(s) 1275
AcsI RAATTY 2 cut(s) 642, 888
AfaI GTAC 2 cut(s) 1085, 1138
AfiI CCNNNNNNNGG 1 cut(s) 628
AgsI TTSAA 9 cut(s) 226, 253, 285, 509, 651, 886, 1013, 1021, 1052
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 2 cut(s) 102, 192
AluBI AGCT 8 cut(s) 110, 505, 525, 661, 743, 753, 788, 1183
AluI AGCT 8 cut(s) 110, 505, 525, 661, 743, 753, 788, 1183
Alw26I GTCTC 4 cut(s) 134, 576, 626, 1301
AlwI GGATC 1 cut(s) 1275
Ama87I CYCGRG 1 cut(s) 1032
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 107, 131, 572
ApoI RAATTY 2 cut(s) 642, 888
Asp700I GAANNNNTTC 1 cut(s) 646
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 109, 1067, 1288
AsuII TTCGAA 2 cut(s) 79, 235
AsuNHI GCTAGC 1 cut(s) 739
AvaI CYCGRG 1 cut(s) 1032
BaeGI GKGCMC 1 cut(s) 173
BaeI ACNNNNGTAYC 2 cut(s) 426, 459
BanI GGYRCC 1 cut(s) 1149
BanII GRGCYC 1 cut(s) 56
BarI GAAGNNNNNNTAC 2 cut(s) 60, 92
BauI CACGAG 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 3 cut(s) 118, 119, 559
BccI CCATC 2 cut(s) 350, 1233
BceAI ACGGC 1 cut(s) 1338
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 2 cut(s) 104, 194
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 134, 576, 626, 1301
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 3 cut(s) 296, 740, 750
BfmI CTRYAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 427
BglI GCCNNNNNGGC 1 cut(s) 51
BisI GCNGC 3 cut(s) 108, 132, 573
BlsI GCNGC 3 cut(s) 109, 133, 574
Bme1390I CCNGG 2 cut(s) 104, 194
BmeT110I CYCGRG 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 444, 1151
BmrFI CCNGG 2 cut(s) 104, 194
BmrI ACTGGG 1 cut(s) 1273
BmsI GCATC 2 cut(s) 364, 820
BmtI GCTAGC 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 1273
BpiI GAAGAC 1 cut(s) 620
Bpu14I TTCGAA 2 cut(s) 79, 235
BpuEI CTTGAG 4 cut(s) 142, 683, 768, 1109
BsaI GGTCTC 1 cut(s) 1301
BsaJI CCNNGG 2 cut(s) 48, 173
BsaWI WCCGGW 1 cut(s) 1065
Bsc4I CCNNNNNNNGG 1 cut(s) 628
Bse1I ACTGG 2 cut(s) 1123, 1268
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 104, 194
BseDI CCNNGG 2 cut(s) 48, 173
BseGI GGATG 2 cut(s) 70, 1225
BseLI CCNNNNNNNGG 1 cut(s) 628
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 4 cut(s) 366, 399, 1071, 1175
BseNI ACTGG 2 cut(s) 1123, 1268
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 1 cut(s) 173
BseXI GCAGC 3 cut(s) 118, 119, 559
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 1149
BsiHKCI CYCGRG 1 cut(s) 1032
BsiSI CCGG 1 cut(s) 1066
BslFI GGGAC 1 cut(s) 1341
BslI CCNNNNNNNGG 1 cut(s) 628
BsmAI GTCTC 4 cut(s) 134, 576, 626, 1301
BsmFI GGGAC 1 cut(s) 1341
BsmI GAATGC 1 cut(s) 1322
BsnI GGCC 1 cut(s) 164
Bso31I GGTCTC 1 cut(s) 1301
BsoBI CYCGRG 1 cut(s) 1032
Bsp119I TTCGAA 2 cut(s) 79, 235
Bsp1286I GDGCHC 2 cut(s) 56, 173
Bsp1407I TGTACA 1 cut(s) 1136
Bsp143I GATC 3 cut(s) 448, 931, 1267
BspACI CCGC 2 cut(s) 151, 332
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 4 cut(s) 365, 400, 1072, 1176
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 2 cut(s) 444, 1151
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 1 cut(s) 1275
BspQI GCTCTTC 1 cut(s) 495
BspT104I TTCGAA 2 cut(s) 79, 235
BspT107I GGYRCC 1 cut(s) 1149
BspTNI GGTCTC 1 cut(s) 1301
BsrDI GCAATG 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 1136
BsrI ACTGG 2 cut(s) 1123, 1268
BssECI CCNNGG 2 cut(s) 48, 173
BssMI GATC 3 cut(s) 448, 931, 1267
BssSI CACGAG 1 cut(s) 1199
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 104, 194
Bst4CI ACNGT 4 cut(s) 145, 316, 853, 947
Bst6I CTCTTC 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 1136
BstBI TTCGAA 2 cut(s) 79, 235
BstC8I GCNNGC 6 cut(s) 272, 527, 596, 741, 745, 755
BstDEI CTNAG 5 cut(s) 352, 408, 1080, 1184, 1374
BstDSI CCRYGG 1 cut(s) 173
BstF5I GGATG 2 cut(s) 70, 1225
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 3 cut(s) 451, 934, 1270
BstMAI GTCTC 4 cut(s) 134, 576, 626, 1301
BstMBI GATC 3 cut(s) 448, 931, 1267
BstMWI GCNNNNNNNGC 4 cut(s) 51, 99, 736, 839
BstNI CCWGG 2 cut(s) 104, 194
BstNSI RCATGY 2 cut(s) 274, 381
BstSCI CCNGG 2 cut(s) 102, 192
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 118, 119, 559
BstV2I GAAGAC 1 cut(s) 620
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 173
BtsCI GGATG 2 cut(s) 70, 1225
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 321, 567, 1130
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 6 cut(s) 272, 527, 596, 741, 745, 755
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 1084, 1137
CviAII CATG 9 cut(s) 180, 271, 378, 497, 692, 764, 809, 895, 1252
CviQI GTAC 2 cut(s) 1084, 1137
DdeI CTNAG 5 cut(s) 352, 408, 1080, 1184, 1374
DpnI GATC 3 cut(s) 450, 933, 1269
DpnII GATC 3 cut(s) 448, 931, 1267
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 1301
Eco88I CYCGRG 1 cut(s) 1032
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 2 cut(s) 102, 192
EcoT14I CCWWGG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 9 cut(s) 183, 274, 381, 500, 695, 767, 812, 898, 1255
FaqI GGGAC 1 cut(s) 1341
FatI CATG 9 cut(s) 179, 270, 377, 496, 691, 763, 808, 894, 1251
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 108, 132, 573
FokI GGATG 2 cut(s) 77, 1212
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 3 cut(s) 108, 132, 573
FspBI CTAG 3 cut(s) 296, 740, 750
GlaI GCGC 1 cut(s) 246
GluI GCNGC 3 cut(s) 108, 132, 573
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 1066
HhaI GCGC 1 cut(s) 247
Hin1II CATG 9 cut(s) 183, 274, 381, 500, 695, 767, 812, 898, 1255
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 1249
HindII GTYRAC 1 cut(s) 1249
HinfI GANTC 5 cut(s) 25, 422, 813, 1072, 1210
HpaII CCGG 1 cut(s) 1066
HphI GGTGA 3 cut(s) 109, 1067, 1288
Hpy166II GTNNAC 1 cut(s) 1249
Hpy188I TCNGA 8 cut(s) 325, 355, 373, 385, 397, 409, 1081, 1209
Hpy188III TCNNGA 3 cut(s) 446, 764, 1174
Hpy8I GTNNAC 1 cut(s) 1249
HpyAV CCTTC 3 cut(s) 259, 863, 1046
HpyCH4III ACNGT 4 cut(s) 145, 316, 853, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 99, 736, 839
HpyF3I CTNAG 5 cut(s) 352, 408, 1080, 1184, 1374
Hsp92II CATG 9 cut(s) 183, 274, 381, 500, 695, 767, 812, 898, 1255
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 3 cut(s) 448, 931, 1267
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 3 cut(s) 118, 119, 559
LweI GCATC 2 cut(s) 364, 820
MaeI CTAG 3 cut(s) 296, 740, 750
MaeIII GTNAC 2 cut(s) 212, 1061
MalI GATC 3 cut(s) 450, 933, 1269
MboI GATC 3 cut(s) 448, 931, 1267
MboII GAAGA 7 cut(s) 249, 512, 625, 909, 1081, 1202, 1268
MhlI GDGCHC 2 cut(s) 56, 173
MluCI AATT 9 cut(s) 81, 203, 367, 452, 537, 642, 846, 888, 1047
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 3 cut(s) 375, 1187, 1253
Mph1103I ATGCAT 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 646
MseI TTAA 4 cut(s) 342, 363, 417, 705
MspI CCGG 1 cut(s) 1066
MspR9I CCNGG 2 cut(s) 104, 194
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 2 cut(s) 104, 194
MwoI GCNNNNNNNGC 4 cut(s) 51, 99, 736, 839
NdeII GATC 3 cut(s) 448, 931, 1267
NheI GCTAGC 1 cut(s) 739
NlaIII CATG 9 cut(s) 183, 274, 381, 500, 695, 767, 812, 898, 1255
NlaIV GGNNCC 2 cut(s) 444, 1151
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 2 cut(s) 274, 381
NspV TTCGAA 2 cut(s) 79, 235
PaeI GCATGC 1 cut(s) 274
PagI TCATGA 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 495
PctI GAATGC 1 cut(s) 1322
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 4 cut(s) 25, 422, 1072, 1210
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 109, 133, 574
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 102, 192
PspGI CCWGG 2 cut(s) 102, 192
PspN4I GGNNCC 2 cut(s) 444, 1151
PspPI GGNCC 1 cut(s) 162
PstI CTGCAG 1 cut(s) 63
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 2 cut(s) 1085, 1138
RsaNI GTAC 2 cut(s) 1084, 1137
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 4 cut(s) 342, 363, 417, 705
SatI GCNGC 3 cut(s) 108, 132, 573
Sau3AI GATC 3 cut(s) 448, 931, 1267
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 2 cut(s) 104, 194
SduI GDGCHC 2 cut(s) 56, 173
SfaNI GCATC 2 cut(s) 364, 820
SfcI CTRYAG 1 cut(s) 59
SfuI TTCGAA 2 cut(s) 79, 235
SmlI CTYRAG 4 cut(s) 157, 662, 783, 1088
SmoI CTYRAG 4 cut(s) 157, 662, 783, 1088
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 274
Sse9I AATT 9 cut(s) 81, 203, 367, 452, 537, 642, 846, 888, 1047
SsiI CCGC 2 cut(s) 151, 332
SspMI CTAG 3 cut(s) 296, 740, 750
StyD4I CCNGG 2 cut(s) 102, 192
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 4 cut(s) 145, 316, 853, 947
TaqI TCGA 2 cut(s) 79, 235
TasI AATT 9 cut(s) 81, 203, 367, 452, 537, 642, 846, 888, 1047
TatI WGTACW 1 cut(s) 1136
TfiI GAWTC 4 cut(s) 25, 422, 1072, 1210
Tru1I TTAA 4 cut(s) 342, 363, 417, 705
Tru9I TTAA 4 cut(s) 342, 363, 417, 705
TscAI CASTG 3 cut(s) 321, 574, 1130
TseI GCWGC 3 cut(s) 107, 131, 572
TspDTI ATGAA 5 cut(s) 17, 342, 513, 1179, 1306
TspGWI ACGGA 2 cut(s) 210, 654
TspRI CASTG 3 cut(s) 321, 574, 1130
Tth111I GACNNNGTC 1 cut(s) 16
XapI RAATTY 2 cut(s) 642, 888
XceI RCATGY 2 cut(s) 274, 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmnI GAANNNNTTC 1 cut(s) 646
XspI CTAG 3 cut(s) 296, 740, 750
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.