Rw5G039300

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011426
Physical Location & Seq
Reverse (-)
69298397 .. 69302835
4439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039300.1

Sequence Viewer

Length: 1218 bp
ATGATATTCTTGCATTTTGGGTATTTGGATGAGATTGTTCTATTTCCCAAAATTTTTATTTACCTGTATCATTATGTGGGACATTTGATTGCCAAAATAGTTGCATTCTTCCAGACGGTATCAAAGCCATTACTAGCCCTCTCCATTGCAACGGCTTTTACTGATGAATTAGAATTCGGTCCTAGAACTTCAGATGATGTCAACAATTCTGATGTGCGTCCAGAAGTACACCCAGAAGTATCCTCTGCAGAGTCTACTTTGGATACAAGCGTTTCTGAAGAAGTTCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTTCATAACGAGCTTGAAAGACAAGGGCTTAGCTTGCCAGAAAGAATTGACGAGGAGGAACTTCGCAGGTTCTACACAGCTGCTAACAGAGACTTCTCGAGCTTTCTGTCTGCAATAAAGAAGACAATCAGGTGGAGAGAGACTTATGGAATTCTTTCGGTCCAGGAGCTTGAGAAATGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCAACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTCGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCCCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACTTGTTCTTCGCTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACTTTTATCCAAGTTTTGAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAATATGTACCGGAAGATTCTCTCTGAGTGCCTAGAGACACTCCCATCATATCTTGGTGGCAAATGCCTCTGCAGAATATGTTCTGATATAAACATGAAAGATATGCAGCAGCCTCGCACAAATGATATTATGAAGAGAGAGCTGAGAGCAAGTGTTTGCGAGGGTGGGAGTTTACACTCTCCCGATCCAACTAGTGAGACTGAGGTTGAACCGAACCATAACTGTGATCAGCTGCTGAGAACTGCCATAGTGAGCATCCTTATGTTTTGGATTTTAATAGCTTTTATTGCTGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

405

Amino Acids

46.13

Weight (kDa)

5.83

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 168 - 315 4.5e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccI GTMKAC 1 cut(s) 254
AclWI GGATC 1 cut(s) 1064
AcsI RAATTY 4 cut(s) 51, 173, 471, 717
AcuI CTGAAG 2 cut(s) 174, 297
AfaI GTAC 4 cut(s) 228, 558, 798, 893
AgsI TTSAA 5 cut(s) 338, 715, 850, 881, 1094
AhlI ACTAGT 1 cut(s) 1076
AjnI CCWGG 2 cut(s) 483, 1189
Alw26I GTCTC 4 cut(s) 405, 455, 914, 1076
AlwI GGATC 1 cut(s) 1064
AlwNI CAGNNNCTG 1 cut(s) 1120
Ama87I CYCGRG 2 cut(s) 418, 861
ApeKI GCWGC 4 cut(s) 401, 991, 994, 1117
ApoI RAATTY 4 cut(s) 51, 173, 471, 717
Asp700I GAANNNNTTC 4 cut(s) 282, 475, 742, 964
AspS9I GGNCC 2 cut(s) 179, 481
AvaI CYCGRG 2 cut(s) 418, 861
AvaII GGWCC 2 cut(s) 179, 481
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BbsI GAAGAC 1 cut(s) 449
BbvI GCAGC 4 cut(s) 388, 1003, 1006, 1104
BccI CCATC 3 cut(s) 303, 595, 937
BceAI ACGGC 3 cut(s) 168, 575, 815
BcgI CGANNNNNNTGC 2 cut(s) 980, 1014
BciT130I CCWGG 2 cut(s) 485, 1191
BciVI GTATCC 2 cut(s) 250, 256
BclI TGATCA 2 cut(s) 634, 1111
BcoDI GTCTC 4 cut(s) 405, 455, 914, 1076
BcuI ACTAGT 1 cut(s) 1076
BfaI CTAG 5 cut(s) 134, 183, 569, 917, 1077
BfmI CTRYAG 2 cut(s) 246, 955
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 2 cut(s) 250, 256
BisI GCNGC 4 cut(s) 402, 992, 995, 1118
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 4 cut(s) 403, 993, 996, 1119
Bme1390I CCNGG 2 cut(s) 485, 1191
Bme18I GGWCC 2 cut(s) 179, 481
BmeT110I CYCGRG 2 cut(s) 418, 861
BmgT120I GGNCC 2 cut(s) 179, 481
BmrFI CCNGG 2 cut(s) 485, 1191
BmsI GCATC 4 cut(s) 308, 585, 649, 1149
BpiI GAAGAC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 350
BpuEI CTTGAG 3 cut(s) 512, 597, 612
BsaJI CCNNGG 1 cut(s) 1189
BsaWI WCCGGW 1 cut(s) 894
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 2 cut(s) 485, 1191
BseDI CCNNGG 1 cut(s) 1189
BseGI GGATG 2 cut(s) 34, 1140
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 4 cut(s) 900, 1019, 1077, 1112
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 4 cut(s) 388, 1003, 1006, 1104
BsiHKCI CYCGRG 2 cut(s) 418, 861
BsiSI CCGG 1 cut(s) 895
BslFI GGGAC 2 cut(s) 93, 1185
BsmAI GTCTC 4 cut(s) 405, 455, 914, 1076
BsmFI GGGAC 2 cut(s) 93, 1185
BsmI GAATGC 1 cut(s) 104
BsoBI CYCGRG 2 cut(s) 418, 861
Bsp143I GATC 4 cut(s) 634, 760, 1069, 1111
Bsp1720I GCTNAGC 1 cut(s) 350
BspCNI CTCAG 4 cut(s) 901, 1020, 1078, 1113
BspHI TCATGA 1 cut(s) 1214
BspMAI CTGCAG 2 cut(s) 250, 959
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 1064
BsrDI GCAATG 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 1189
BssMI GATC 4 cut(s) 634, 760, 1069, 1111
Bst2UI CCWGG 2 cut(s) 485, 1191
Bst4CI ACNGT 4 cut(s) 118, 682, 776, 1109
Bst6I CTCTTC 1 cut(s) 1013
BstC8I GCNNGC 3 cut(s) 356, 574, 584
BstDEI CTNAG 5 cut(s) 350, 909, 1028, 1086, 1121
BstF5I GGATG 2 cut(s) 34, 1140
BstKTI GATC 4 cut(s) 637, 763, 1072, 1114
BstMAI GTCTC 4 cut(s) 405, 455, 914, 1076
BstMBI GATC 4 cut(s) 634, 760, 1069, 1111
BstMWI GCNNNNNNNGC 2 cut(s) 355, 1172
BstNI CCWGG 2 cut(s) 485, 1191
BstSCI CCNGG 2 cut(s) 483, 1189
BstSFI CTRYAG 2 cut(s) 246, 955
BstV1I GCAGC 4 cut(s) 388, 1003, 1006, 1104
BstV2I GAAGAC 1 cut(s) 449
BsuI GTATCC 2 cut(s) 250, 256
BtsCI GGATG 2 cut(s) 34, 1140
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 356, 574, 584
CaiI CAGNNNCTG 1 cut(s) 1120
CciI TCATGA 1 cut(s) 1214
Cfr13I GGNCC 2 cut(s) 179, 481
CseI GACGC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 227, 557, 797, 892
CviAII CATG 5 cut(s) 521, 638, 724, 979, 1215
CviQI GTAC 4 cut(s) 227, 557, 797, 892
DdeI CTNAG 5 cut(s) 350, 909, 1028, 1086, 1121
DpnI GATC 4 cut(s) 636, 762, 1071, 1113
DpnII GATC 4 cut(s) 634, 760, 1069, 1111
Eam1104I CTCTTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 1013
Eco47I GGWCC 2 cut(s) 179, 481
Eco57I CTGAAG 2 cut(s) 174, 297
Eco88I CYCGRG 2 cut(s) 418, 861
EcoRI GAATTC 2 cut(s) 173, 471
EcoRII CCWGG 2 cut(s) 483, 1189
FaeI CATG 5 cut(s) 524, 641, 727, 982, 1218
FaqI GGGAC 2 cut(s) 93, 1185
FatI CATG 5 cut(s) 520, 637, 723, 978, 1214
FbaI TGATCA 2 cut(s) 634, 1111
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 4 cut(s) 402, 992, 995, 1118
FokI GGATG 2 cut(s) 41, 1127
Fsp4HI GCNGC 4 cut(s) 402, 992, 995, 1118
FspBI CTAG 5 cut(s) 134, 183, 569, 917, 1077
GluI GCNGC 4 cut(s) 402, 992, 995, 1118
HapII CCGG 1 cut(s) 895
HgaI GACGC 1 cut(s) 206
Hin1II CATG 5 cut(s) 524, 641, 727, 982, 1218
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 251, 642, 901
HpaII CCGG 1 cut(s) 895
Hpy166II GTNNAC 4 cut(s) 202, 229, 255, 1058
Hpy188I TCNGA 6 cut(s) 193, 211, 277, 304, 910, 970
Hpy188III TCNNGA 6 cut(s) 112, 221, 418, 593, 1067, 1215
Hpy8I GTNNAC 4 cut(s) 202, 229, 255, 1058
HpyAV CCTTC 2 cut(s) 692, 875
HpyCH4III ACNGT 4 cut(s) 118, 682, 776, 1109
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 1172
HpyF3I CTNAG 5 cut(s) 350, 909, 1028, 1086, 1121
Hsp92II CATG 5 cut(s) 524, 641, 727, 982, 1218
Ksp22I TGATCA 2 cut(s) 634, 1111
Kzo9I GATC 4 cut(s) 634, 760, 1069, 1111
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 4 cut(s) 388, 1003, 1006, 1104
LweI GCATC 4 cut(s) 308, 585, 649, 1149
MaeI CTAG 5 cut(s) 134, 183, 569, 917, 1077
MalI GATC 4 cut(s) 636, 762, 1071, 1113
MboI GATC 4 cut(s) 634, 760, 1069, 1111
MboII GAAGA 8 cut(s) 100, 290, 454, 738, 910, 1030, 1200, 1203
MlyI GAGTC 2 cut(s) 260, 651
MmeI TCCRAC 1 cut(s) 1097
MroXI GAANNNNTTC 4 cut(s) 282, 475, 742, 964
MseI TTAA 1 cut(s) 1160
MslI CAYNNNNRTG 2 cut(s) 1107, 1145
MspA1I CMGCKG 2 cut(s) 401, 1117
MspI CCGG 1 cut(s) 895
MspR9I CCNGG 2 cut(s) 485, 1191
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 2 cut(s) 485, 1191
MwoI GCNNNNNNNGC 2 cut(s) 355, 1172
NdeII GATC 4 cut(s) 634, 760, 1069, 1111
NlaIII CATG 5 cut(s) 524, 641, 727, 982, 1218
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 1 cut(s) 1214
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 4 cut(s) 282, 475, 742, 964
PfeI GAWTC 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 4 cut(s) 403, 993, 996, 1119
PleI GAGTC 2 cut(s) 259, 650
PpsI GAGTC 2 cut(s) 259, 650
Psp6I CCWGG 2 cut(s) 483, 1189
PspGI CCWGG 2 cut(s) 483, 1189
PspPI GGNCC 2 cut(s) 179, 481
PstI CTGCAG 2 cut(s) 250, 959
PstNI CAGNNNCTG 1 cut(s) 1120
PvuII CAGCTG 2 cut(s) 401, 1117
RsaI GTAC 4 cut(s) 228, 558, 798, 893
RsaNI GTAC 4 cut(s) 227, 557, 797, 892
RseI CAYNNNNRTG 2 cut(s) 1107, 1145
SaqAI TTAA 1 cut(s) 1160
SatI GCNGC 4 cut(s) 402, 992, 995, 1118
Sau3AI GATC 4 cut(s) 634, 760, 1069, 1111
Sau96I GGNCC 2 cut(s) 179, 481
SchI GAGTC 2 cut(s) 260, 651
ScrFI CCNGG 2 cut(s) 485, 1191
SfaNI GCATC 4 cut(s) 308, 585, 649, 1149
SfcI CTRYAG 2 cut(s) 246, 955
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 2 cut(s) 179, 481
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 1107, 1145
SmlI CTYRAG 4 cut(s) 418, 491, 612, 627
SmoI CTYRAG 4 cut(s) 418, 491, 612, 627
SpeI ACTAGT 1 cut(s) 1076
SspMI CTAG 5 cut(s) 134, 183, 569, 917, 1077
StyD4I CCNGG 2 cut(s) 483, 1189
TaaI ACNGT 4 cut(s) 118, 682, 776, 1109
TaqI TCGA 1 cut(s) 419
TaqII GACCGA 2 cut(s) 167, 469
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 1 cut(s) 901
Tru1I TTAA 1 cut(s) 1160
Tru9I TTAA 1 cut(s) 1160
TseI GCWGC 4 cut(s) 401, 991, 994, 1117
TspDTI ATGAA 6 cut(s) 180, 314, 638, 995, 1031, 1203
VpaK11BI GGWCC 2 cut(s) 179, 481
XapI RAATTY 4 cut(s) 51, 173, 471, 717
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 254
XmnI GAANNNNTTC 4 cut(s) 282, 475, 742, 964
XspI CTAG 5 cut(s) 134, 183, 569, 917, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.