MD11G1142800.v1.1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011426
Physical Location & Seq
Forward (+)
13316143 .. 13320856
4714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1142800.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGAGTGGAAAGAGGAAGTCGCAGAATCATATAGTTGCTTCTCGTTCCAAGGGCACTCTGCAGGATGCAGTGTCGCTTCGAATTGGGAATGGCGTGGTGAGCCAGGCCGCGTTGTTTCTGCTCAAAATGGCTGCATTGGAGATAGTAAGGCGGTTCTCAAGGGCCAAATGCCCGTGGGCATGGCGTGGCTTCCAGGCTTTGCAATTTCTGTGTTACCCTCCATTCAAATGGCTTCAAAGATTTGCGCCCTTCAAGGCTTTGGTCAATGGCATGCAGGTAGTTTCGAAACCATTACTAGTGCTTTCCATTGCAACGACATTCTCTGATGAAGCAGAGCTCTTTGATAGAACCTCTGATGGCATCAACAATTCTGATGCAAACATAGAAGTGCAGTCAGAAGTGTTGTCTGAGTGTATTAACGATTCTACTTTGGATCCAAGGGCTCCTGATCAATTTCCTCAAAATGTAGAACCTGAAATGTGGTTGGTAGAACTCCATAAAGAGCTTGAAAGACAAGGGATTAGCTTGCCAGAAAGAATTGATAAGGGGGAACTTCGTAGGTTTTACACTGCTGCAAACGGAGACTTCTCTTGCTTGCTATCATCAGTAAAGAAGACAATCCGTTGGAGGGAGACTTATGGAATACTTTCAGTACAGGAGCTTGAGACGTGGTCAAATGTGGTGTTCTGGCATGGATTTGATGTTAAACACCGACCTTGCCTCATAGTACGACTTGGGCTAGCTTGCACTAGCTTCCCATCTCATGATAGACCTCGCTTTGCTCAAGCAATTATTTCCCAAGTAGAGAATGGAGTCTATCATTTGCTTGATGCCACCAATGCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACGCCGTTGAAAATTCCCATGCAAGTTATGAGGACCTGTTCTTCTCTTTTGCAAGATCACTTCCCCAACCGTCTTGGCTGTCTGTTTGTTATTCGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACCTTTATTCAAGTTTTGAAACCTAATACTCGAGAGAAGTTGAGAATTGAAGGTAAGAGGTACCAGAAGATTCTCTCTGAGTACCTTGAGGCACTTCCATTTTATCTTGGTGGCAATTGCCTGTGCAAAATATGTACAGAGATGCAGCATCCTCGCCCAAATGAAGTTGTCAAGACACAGCTGAGAGCAAATTTGCACGAGGGTCGGAATCCACTTCCAGCCCATCCAACTTATGAGATTGCGGTTGACCCGGAAGGTAACTGGGAGCAGTTGGTGAGGACTGCCATAATTGGTCTCCTTATGGTATGGGTTTCCATAGCATTCCTTTCTTTAATCTTTGATCCTGAAAGCCGTCCCTTTCCCTTTTCATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

460

Amino Acids

52.25

Weight (kDa)

8.66

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 225 - 372 1e-26 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
Acc65I GGTACC 1 cut(s) 1062
AccB1I GGYRCC 1 cut(s) 1062
AccII CGCG 1 cut(s) 110
AciI CCGC 3 cut(s) 108, 151, 1244
AclWI GGATC 3 cut(s) 428, 441, 1337
AcsI RAATTY 2 cut(s) 888, 1192
AfaI GTAC 5 cut(s) 654, 729, 1064, 1085, 1138
AfiI CCNNNNNNNGG 1 cut(s) 628
AgsI TTSAA 8 cut(s) 226, 236, 253, 509, 886, 1013, 1021, 1052
AhlI ACTAGT 1 cut(s) 295
AjiI CACGTC 1 cut(s) 669
AjnI CCWGG 2 cut(s) 102, 192
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AluBI AGCT 7 cut(s) 337, 505, 525, 661, 743, 753, 1183
AluI AGCT 7 cut(s) 337, 505, 525, 661, 743, 753, 1183
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 4 cut(s) 576, 626, 659, 1301
AlwI GGATC 3 cut(s) 428, 441, 1337
Ama87I CYCGRG 1 cut(s) 1032
AoxI GGCC 2 cut(s) 105, 162
ApeKI GCWGC 3 cut(s) 131, 572, 1147
ApoI RAATTY 2 cut(s) 888, 1192
Asp700I GAANNNNTTC 1 cut(s) 646
Asp718I GGTACC 1 cut(s) 1062
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 162, 909
AsuC2I CCSGG 1 cut(s) 1253
AsuHPI GGTGA 2 cut(s) 109, 1288
AsuII TTCGAA 2 cut(s) 79, 284
AsuNHI GCTAGC 1 cut(s) 739
AvaI CYCGRG 1 cut(s) 1032
AvaII GGWCC 1 cut(s) 909
BaeGI GKGCMC 1 cut(s) 56
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 1062
BanII GRGCYC 2 cut(s) 339, 445
BauI CACGAG 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 118, 559, 1159
BccI CCATC 3 cut(s) 350, 766, 1233
BceAI ACGGC 2 cut(s) 865, 1338
BcgI CGANNNNNNTGC 4 cut(s) 1136, 1170, 1187, 1221
BciT130I CCWGG 2 cut(s) 104, 194
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 1253
BcoDI GTCTC 4 cut(s) 576, 626, 659, 1301
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 3 cut(s) 296, 740, 750
BfmI CTRYAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 4 cut(s) 108, 132, 573, 1148
BlsI GCNGC 4 cut(s) 109, 133, 574, 1149
Bme1390I CCNGG 3 cut(s) 104, 194, 1253
Bme18I GGWCC 1 cut(s) 909
BmeT110I CYCGRG 1 cut(s) 1032
BmgBI CACGTC 1 cut(s) 669
BmgT120I GGNCC 2 cut(s) 162, 909
BmiI GGNNCC 3 cut(s) 435, 444, 1064
BmrFI CCNGG 3 cut(s) 104, 194, 1253
BmrI ACTGGG 1 cut(s) 1273
BmsI GCATC 6 cut(s) 55, 364, 369, 820, 1134, 1159
BmtI GCTAGC 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 1273
BpiI GAAGAC 1 cut(s) 620
Bpu14I TTCGAA 2 cut(s) 79, 284
BpuEI CTTGAG 4 cut(s) 142, 683, 768, 1109
BpuMI CCSGG 1 cut(s) 1253
BsaI GGTCTC 1 cut(s) 1301
BsaJI CCNNGG 3 cut(s) 48, 173, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 628
Bse1I ACTGG 1 cut(s) 1268
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 104, 194
BseDI CCNNGG 3 cut(s) 48, 173, 437
BseGI GGATG 3 cut(s) 70, 1150, 1225
BseLI CCNNNNNNNGG 1 cut(s) 628
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 3 cut(s) 399, 1071, 1175
BseNI ACTGG 1 cut(s) 1268
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 3 cut(s) 118, 559, 1159
BsgI GTGCAG 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 107, 164
BshNI GGYRCC 1 cut(s) 1062
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 1032
BsiSI CCGG 1 cut(s) 1253
BslFI GGGAC 1 cut(s) 1341
BslI CCNNNNNNNGG 1 cut(s) 628
BsmAI GTCTC 4 cut(s) 576, 626, 659, 1301
BsmBI CGTCTC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 1341
BsmI GAATGC 1 cut(s) 1322
BsnI GGCC 2 cut(s) 107, 164
Bso31I GGTCTC 1 cut(s) 1301
BsoBI CYCGRG 1 cut(s) 1032
Bsp119I TTCGAA 2 cut(s) 79, 284
Bsp1286I GDGCHC 3 cut(s) 56, 339, 445
Bsp1407I TGTACA 1 cut(s) 1136
Bsp143I GATC 4 cut(s) 433, 448, 931, 1342
BspACI CCGC 3 cut(s) 108, 151, 1244
BspANI GGCC 2 cut(s) 107, 164
BspCNI CTCAG 3 cut(s) 400, 1072, 1176
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 3 cut(s) 435, 444, 1064
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 3 cut(s) 428, 441, 1337
BspT104I TTCGAA 2 cut(s) 79, 284
BspT107I GGYRCC 1 cut(s) 1062
BspTNI GGTCTC 1 cut(s) 1301
BsrDI GCAATG 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 1136
BsrI ACTGG 1 cut(s) 1268
BssECI CCNNGG 3 cut(s) 48, 173, 437
BssMI GATC 4 cut(s) 433, 448, 931, 1342
BssSI CACGAG 1 cut(s) 1199
BssT1I CCWWGG 2 cut(s) 48, 437
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 104, 194
Bst4CI ACNGT 2 cut(s) 853, 947
BstAUI TGTACA 1 cut(s) 1136
BstBI TTCGAA 2 cut(s) 79, 284
BstC8I GCNNGC 5 cut(s) 272, 527, 596, 741, 745
BstDEI CTNAG 3 cut(s) 408, 1080, 1184
BstDSI CCRYGG 1 cut(s) 173
BstF5I GGATG 3 cut(s) 70, 1150, 1225
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 436, 451, 934, 1345
BstMAI GTCTC 4 cut(s) 576, 626, 659, 1301
BstMBI GATC 4 cut(s) 433, 448, 931, 1342
BstMWI GCNNNNNNNGC 2 cut(s) 99, 839
BstNI CCWGG 2 cut(s) 104, 194
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 3 cut(s) 102, 192, 1251
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 3 cut(s) 118, 559, 1159
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 228
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 107, 164
BtgI CCRYGG 1 cut(s) 173
BtrI CACGTC 1 cut(s) 669
BtsCI GGATG 3 cut(s) 70, 1150, 1225
BtsI GCAGTG 2 cut(s) 75, 567
BtsIMutI CAGTG 2 cut(s) 75, 567
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 5 cut(s) 272, 527, 596, 741, 745
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 2 cut(s) 162, 909
Csp6I GTAC 5 cut(s) 653, 728, 1063, 1084, 1137
CviAII CATG 5 cut(s) 180, 271, 692, 764, 895
CviQI GTAC 5 cut(s) 653, 728, 1063, 1084, 1137
DdeI CTNAG 3 cut(s) 408, 1080, 1184
DpnI GATC 4 cut(s) 435, 450, 933, 1344
DpnII GATC 4 cut(s) 433, 448, 931, 1342
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 48, 437
Eco24I GRGCYC 2 cut(s) 339, 445
Eco31I GGTCTC 1 cut(s) 1301
Eco47I GGWCC 1 cut(s) 909
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 1032
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 909
EcoRII CCWGG 2 cut(s) 102, 192
EcoT14I CCWWGG 2 cut(s) 48, 437
EcoT38I GRGCYC 2 cut(s) 339, 445
ErhI CCWWGG 2 cut(s) 48, 437
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 5 cut(s) 183, 274, 695, 767, 898
FaqI GGGAC 1 cut(s) 1341
FatI CATG 5 cut(s) 179, 270, 691, 763, 894
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 108, 132, 573, 1148
FokI GGATG 3 cut(s) 77, 1137, 1212
FriOI GRGCYC 2 cut(s) 339, 445
Fsp4HI GCNGC 4 cut(s) 108, 132, 573, 1148
FspBI CTAG 3 cut(s) 296, 740, 750
GlaI GCGC 1 cut(s) 246
GluI GCNGC 4 cut(s) 108, 132, 573, 1148
HaeIII GGCC 2 cut(s) 107, 164
HapII CCGG 1 cut(s) 1253
HhaI GCGC 1 cut(s) 247
Hin1II CATG 5 cut(s) 183, 274, 695, 767, 898
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 1249
HindII GTYRAC 1 cut(s) 1249
HinfI GANTC 5 cut(s) 25, 422, 813, 1072, 1210
HpaII CCGG 1 cut(s) 1253
HphI GGTGA 2 cut(s) 109, 1288
Hpy166II GTNNAC 1 cut(s) 1249
Hpy188I TCNGA 7 cut(s) 325, 355, 373, 397, 409, 1081, 1209
Hpy188III TCNNGA 5 cut(s) 446, 764, 1034, 1174, 1346
Hpy8I GTNNAC 1 cut(s) 1249
HpyAV CCTTC 4 cut(s) 259, 863, 1046, 1250
HpyCH4III ACNGT 2 cut(s) 853, 947
HpyCH4IV ACGT 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 839
HpyF3I CTNAG 3 cut(s) 408, 1080, 1184
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 5 cut(s) 183, 274, 695, 767, 898
HspAI GCGC 1 cut(s) 245
KpnI GGTACC 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 4 cut(s) 433, 448, 931, 1342
LmnI GCTCC 3 cut(s) 448, 658, 1267
Lsp1109I GCAGC 3 cut(s) 118, 559, 1159
LweI GCATC 6 cut(s) 55, 364, 369, 820, 1134, 1159
MaeI CTAG 3 cut(s) 296, 740, 750
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 2 cut(s) 212, 1259
MalI GATC 4 cut(s) 435, 450, 933, 1344
MboI GATC 4 cut(s) 433, 448, 931, 1342
MboII GAAGA 3 cut(s) 625, 909, 1081
MfeI CAATTG 1 cut(s) 1117
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 3 cut(s) 56, 339, 445
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 3 cut(s) 605, 1187, 1253
MroXI GAANNNNTTC 1 cut(s) 646
MseI TTAA 3 cut(s) 417, 705, 1334
MslI CAYNNNNRTG 3 cut(s) 226, 386, 1375
MspA1I CMGCKG 1 cut(s) 1183
MspI CCGG 1 cut(s) 1253
MspR9I CCNGG 3 cut(s) 104, 194, 1253
MunI CAATTG 1 cut(s) 1117
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 2 cut(s) 104, 194
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 99, 839
NciI CCSGG 1 cut(s) 1253
NdeII GATC 4 cut(s) 433, 448, 931, 1342
NheI GCTAGC 1 cut(s) 739
NlaIII CATG 5 cut(s) 183, 274, 695, 767, 898
NlaIV GGNNCC 3 cut(s) 435, 444, 1064
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 2 cut(s) 79, 284
PaeI GCATGC 1 cut(s) 274
PaeR7I CTCGAG 1 cut(s) 1032
PagI TCATGA 1 cut(s) 763
PctI GAATGC 1 cut(s) 1322
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 4 cut(s) 25, 422, 1072, 1210
PflFI GACNNNGTC 1 cut(s) 670
PkrI GCNGC 4 cut(s) 109, 133, 574, 1149
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
PpuMI RGGWCCY 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 2 cut(s) 102, 192
PspGI CCWGG 2 cut(s) 102, 192
PspN4I GGNNCC 3 cut(s) 435, 444, 1064
PspPI GGNCC 2 cut(s) 162, 909
PspPPI RGGWCCY 1 cut(s) 909
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 1183
RsaI GTAC 5 cut(s) 654, 729, 1064, 1085, 1138
RsaNI GTAC 5 cut(s) 653, 728, 1063, 1084, 1137
RseI CAYNNNNRTG 3 cut(s) 226, 386, 1375
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 417, 705, 1334
SatI GCNGC 4 cut(s) 108, 132, 573, 1148
Sau3AI GATC 4 cut(s) 433, 448, 931, 1342
Sau96I GGNCC 2 cut(s) 162, 909
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 3 cut(s) 104, 194, 1253
SduI GDGCHC 3 cut(s) 56, 339, 445
SfaNI GCATC 6 cut(s) 55, 364, 369, 820, 1134, 1159
SfcI CTRYAG 1 cut(s) 59
Sfr274I CTCGAG 1 cut(s) 1032
SfuI TTCGAA 2 cut(s) 79, 284
SinI GGWCC 1 cut(s) 909
SlaI CTCGAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 3 cut(s) 226, 386, 1375
SmlI CTYRAG 5 cut(s) 157, 662, 783, 1032, 1088
SmoI CTYRAG 5 cut(s) 157, 662, 783, 1032, 1088
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 274
SsiI CCGC 3 cut(s) 108, 151, 1244
SspMI CTAG 3 cut(s) 296, 740, 750
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 3 cut(s) 102, 192, 1251
StyI CCWWGG 2 cut(s) 48, 437
TaaI ACNGT 2 cut(s) 853, 947
TaiI ACGT 1 cut(s) 671
TaqI TCGA 3 cut(s) 79, 284, 1033
TatI WGTACW 2 cut(s) 652, 1136
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 4 cut(s) 25, 422, 1072, 1210
Tru1I TTAA 3 cut(s) 417, 705, 1334
Tru9I TTAA 3 cut(s) 417, 705, 1334
TscAI CASTG 2 cut(s) 75, 574
TseI GCWGC 3 cut(s) 131, 572, 1147
TspDTI ATGAA 3 cut(s) 342, 1179, 1359
TspGWI ACGGA 2 cut(s) 594, 611
TspRI CASTG 2 cut(s) 75, 574
Tth111I GACNNNGTC 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 909
XapI RAATTY 2 cut(s) 888, 1192
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XhoI CTCGAG 1 cut(s) 1032
XmnI GAANNNNTTC 1 cut(s) 646
XspI CTAG 3 cut(s) 296, 740, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.