RchiOBHm_Chr5g0063781

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011426
Physical Location & Seq
Reverse (-)
69401609 .. 69405847
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33985

Sequence Viewer

Length: 1431 bp
ATGGGAGACTCGTCTTCGTATGGGATCCCTAAAGAGGCCAAGCTTGTTATGTCTGGGAAAAAGCAGTCTCCGAATTACTTAGTTGCTTCGCGCTCTAAGGCCTCTTTGCAAAGCTTGGGAGCACTTGGAATTGGTAATAATGGTGTGGTCAGCCAGGCAGCAGTGTTTCTGCTCAAAGTGGCTGCATTGGAGGCAGCGCGGAGGCTCTTCAGGGCCAAGTGCCCCTGGCTGTGGCGCGGCTTCCAGGCTTTGCAATTTCTGTGTTATCCACCGTTTAAGTGGCTTCGGAGATTCGCTCCCTTCAAGGCTCTGGTCGACAACTTGCAGACGGTATCGAAGCCATTACTAGCCCTCTCCATTGCAACGGCTTTTACTGATGAATTAGAATTCGGTCCTAGAGCTTCAGATGATGTCAACAATTCTGATGTGCGTCCAGAAGTACAGCCAGAAGTATTCTCTGCAGAGTCTGCTTTGGATACAAGTGTTTCTGAAGAAGTGCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTTCATAACGAGCTCGAAAGACAAGGCCTTAGCTTGCCAGAAAGAATTGATGAGGAGGAACTTCGCAGGTTCTACACAGCTGCTAACAGAGACTTCTCAAGCTTTCTGTCTGCAATAAAGAAGACGATCAGGTGGAGAGAGACGTATGGAATTCTTTCAGTACAGGAGCTTGAGAGATGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTTGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACCTGTTCTTCGCTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACTTTCATCCAAGTTTTGAAACCTTACACCAGAGAAAAGTTGAGAATTGAAGGGAAGATGTACTGGAAGATTCTCTCTGAGTGCCTAGAGACACTCCCTTCATGTCTTGGTGGCAAATGCATGTGCAGAATATGTTCAGAAATTAACACGAAAGATATGCAGCAGCCTCGCACAAATGATATTATGAAGAGAGAGCTGAAAGTGTGTGCGCGCGAGGATGGGAGTTTATGCTCTCCCAGTCCAACTTGTGAGACCGAGGTTGAACCGAACCATTACTGTGATCAGCTGCTGAGAACTGCCATAGTGAGCATCCTTATGTTTTGGATTTTAGTAGCTTTTATTGCTGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

476

Amino Acids

53.63

Weight (kDa)

8.25

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 239 - 386 3.6e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 315
AccII CGCG 5 cut(s) 91, 199, 237, 1255, 1257
AciI CCGC 2 cut(s) 199, 237
AclWI GGATC 2 cut(s) 19, 32
AcsI RAATTY 3 cut(s) 386, 684, 930
AcuI CTGAAG 3 cut(s) 193, 387, 510
AfaI GTAC 5 cut(s) 441, 696, 771, 1011, 1106
AfiI CCNNNNNNNGG 2 cut(s) 34, 231
AgsI TTSAA 5 cut(s) 304, 928, 1063, 1094, 1307
AjnI CCWGG 4 cut(s) 153, 224, 243, 1402
Alw21I GWGCWC 2 cut(s) 124, 549
Alw26I GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
AlwI GGATC 2 cut(s) 19, 32
AlwNI CAGNNNCTG 2 cut(s) 467, 1333
AoxI GGCC 4 cut(s) 36, 99, 213, 559
ApeKI GCWGC 7 cut(s) 158, 182, 194, 614, 1204, 1207, 1330
ApoI RAATTY 3 cut(s) 386, 684, 930
Asp700I GAANNNNTTC 3 cut(s) 688, 955, 1177
AspLEI GCGC 5 cut(s) 93, 199, 237, 1255, 1257
AspS9I GGNCC 2 cut(s) 213, 392
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BamHI GGATCC 1 cut(s) 24
BanII GRGCYC 1 cut(s) 549
BbsI GAAGAC 2 cut(s) 6, 662
Bbv12I GWGCWC 2 cut(s) 124, 549
BbvI GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
BccI CCATC 4 cut(s) 516, 705, 808, 1256
BceAI ACGGC 3 cut(s) 381, 788, 1028
BcgI CGANNNNNNTGC 4 cut(s) 1183, 1193, 1217, 1227
BciT130I CCWGG 4 cut(s) 155, 226, 245, 1404
BciVI GTATCC 1 cut(s) 469
BclI TGATCA 2 cut(s) 847, 1324
BcoDI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BfaI CTAG 4 cut(s) 347, 396, 782, 1130
BfmI CTRYAG 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 591
BfuI GTATCC 1 cut(s) 469
BisI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
BlsI GCNGC 8 cut(s) 160, 184, 196, 239, 616, 1206, 1209, 1332
Bme1390I CCNGG 4 cut(s) 155, 226, 245, 1404
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 2 cut(s) 213, 392
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 4 cut(s) 155, 226, 245, 1404
BmrI ACTGGG 1 cut(s) 1275
BmsI GCATC 4 cut(s) 521, 798, 862, 1362
BmuI ACTGGG 1 cut(s) 1275
BpiI GAAGAC 2 cut(s) 6, 662
BplI GAGNNNNNCTC 2 cut(s) 280, 312
Bpu10I CCTNAGC 1 cut(s) 563
BpuEI CTTGAG 4 cut(s) 616, 725, 810, 825
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 3 cut(s) 224, 1299, 1402
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 231
Bse1I ACTGG 2 cut(s) 1112, 1281
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 4 cut(s) 155, 226, 245, 1404
BseDI CCNNGG 3 cut(s) 224, 1299, 1402
BseGI GGATG 3 cut(s) 1050, 1267, 1353
BseLI CCNNNNNNNGG 2 cut(s) 34, 231
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 1113, 1325
BseNI ACTGG 2 cut(s) 1112, 1281
BsePI GCGCGC 1 cut(s) 1253
BseRI GAGGAG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
BsgI GTGCAG 1 cut(s) 1189
Bsh1236I CGCG 5 cut(s) 91, 199, 237, 1255, 1257
BshFI GGCC 4 cut(s) 38, 101, 215, 561
BsiHKAI GWGCWC 2 cut(s) 124, 549
BslFI GGGAC 1 cut(s) 1398
BslI CCNNNNNNNGG 2 cut(s) 34, 231
BsmAI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 1 cut(s) 1398
BsnI GGCC 4 cut(s) 38, 101, 215, 561
Bso31I GGTCTC 1 cut(s) 1289
Bsp1286I GDGCHC 3 cut(s) 124, 224, 549
Bsp143I GATC 5 cut(s) 24, 660, 847, 973, 1324
BspACI CCGC 2 cut(s) 199, 237
BspANI GGCC 4 cut(s) 38, 101, 215, 561
BspCNI CTCAG 2 cut(s) 1114, 1326
BspFNI CGCG 5 cut(s) 91, 199, 237, 1255, 1257
BspHI TCATGA 1 cut(s) 1427
BspLI GGNNCC 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 591
BspPI GGATC 2 cut(s) 19, 32
BspQI GCTCTTC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 1289
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 2 cut(s) 1112, 1281
BssECI CCNNGG 3 cut(s) 224, 1299, 1402
BssHII GCGCGC 1 cut(s) 1253
BssMI GATC 5 cut(s) 24, 660, 847, 973, 1324
Bst2UI CCWGG 4 cut(s) 155, 226, 245, 1404
Bst4CI ACNGT 5 cut(s) 273, 331, 895, 989, 1322
Bst6I CTCTTC 2 cut(s) 212, 1226
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 569, 787, 797, 1255
BstDEI CTNAG 5 cut(s) 79, 96, 563, 1122, 1334
BstF5I GGATG 3 cut(s) 1050, 1267, 1353
BstFNI CGCG 5 cut(s) 91, 199, 237, 1255, 1257
BstHHI GCGC 5 cut(s) 93, 199, 237, 1255, 1257
BstKTI GATC 5 cut(s) 27, 663, 850, 976, 1327
BstMAI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BstMBI GATC 5 cut(s) 24, 660, 847, 973, 1324
BstMWI GCNNNNNNNGC 3 cut(s) 191, 467, 1385
BstNI CCWGG 4 cut(s) 155, 226, 245, 1404
BstNSI RCATGY 1 cut(s) 1168
BstSCI CCNGG 4 cut(s) 153, 224, 243, 1402
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 5 cut(s) 91, 199, 237, 1255, 1257
BstV1I GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
BstV2I GAAGAC 2 cut(s) 6, 662
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 4 cut(s) 38, 101, 215, 561
BtsCI GGATG 3 cut(s) 1050, 1267, 1353
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 591
Cac8I GCNNGC 4 cut(s) 569, 787, 797, 1255
CaiI CAGNNNCTG 2 cut(s) 467, 1333
CciI TCATGA 1 cut(s) 1427
CfoI GCGC 5 cut(s) 93, 199, 237, 1255, 1257
Cfr13I GGNCC 2 cut(s) 213, 392
CseI GACGC 1 cut(s) 419
Csp6I GTAC 5 cut(s) 440, 695, 770, 1010, 1105
CviAII CATG 6 cut(s) 734, 851, 937, 1146, 1165, 1428
CviQI GTAC 5 cut(s) 440, 695, 770, 1010, 1105
DdeI CTNAG 5 cut(s) 79, 96, 563, 1122, 1334
DpnI GATC 5 cut(s) 26, 662, 849, 975, 1326
DpnII GATC 5 cut(s) 24, 660, 847, 973, 1324
Eam1104I CTCTTC 2 cut(s) 212, 1226
EarI CTCTTC 2 cut(s) 212, 1226
Ecl136II GAGCTC 1 cut(s) 547
Eco147I AGGCCT 2 cut(s) 101, 561
Eco24I GRGCYC 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 3 cut(s) 193, 387, 510
EcoICRI GAGCTC 1 cut(s) 547
EcoRI GAATTC 2 cut(s) 386, 684
EcoRII CCWGG 4 cut(s) 153, 224, 243, 1402
EcoT22I ATGCAT 1 cut(s) 1166
EcoT38I GRGCYC 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 6 cut(s) 737, 854, 940, 1149, 1168, 1431
FaqI GGGAC 1 cut(s) 1398
FatI CATG 6 cut(s) 733, 850, 936, 1145, 1164, 1427
FbaI TGATCA 2 cut(s) 847, 1324
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
FokI GGATG 3 cut(s) 1037, 1274, 1340
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
FspBI CTAG 4 cut(s) 347, 396, 782, 1130
GlaI GCGC 5 cut(s) 92, 198, 236, 1254, 1256
GluI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
HaeIII GGCC 4 cut(s) 38, 101, 215, 561
HgaI GACGC 1 cut(s) 419
HhaI GCGC 5 cut(s) 93, 199, 237, 1255, 1257
Hin1II CATG 6 cut(s) 737, 854, 940, 1149, 1168, 1431
Hin6I GCGC 5 cut(s) 91, 197, 235, 1253, 1255
HinP1I GCGC 5 cut(s) 91, 197, 235, 1253, 1255
HincII GTYRAC 2 cut(s) 316, 415
HindII GTYRAC 2 cut(s) 316, 415
HindIII AAGCTT 3 cut(s) 41, 112, 634
HinfI GANTC 5 cut(s) 8, 291, 464, 855, 1114
Hpy166II GTNNAC 2 cut(s) 316, 415
Hpy188I TCNGA 8 cut(s) 72, 288, 406, 424, 490, 517, 1123, 1183
Hpy188III TCNNGA 3 cut(s) 434, 806, 1428
Hpy8I GTNNAC 2 cut(s) 316, 415
HpyAV CCTTC 4 cut(s) 310, 905, 1088, 1152
HpyCH4III ACNGT 5 cut(s) 273, 331, 895, 989, 1322
HpyCH4IV ACGT 1 cut(s) 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 467, 1385
HpyF3I CTNAG 5 cut(s) 79, 96, 563, 1122, 1334
HpySE526I ACGT 1 cut(s) 677
Hsp92II CATG 6 cut(s) 737, 854, 940, 1149, 1168, 1431
HspAI GCGC 5 cut(s) 91, 197, 235, 1253, 1255
Ksp22I TGATCA 2 cut(s) 847, 1324
Kzo9I GATC 5 cut(s) 24, 660, 847, 973, 1324
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 3 cut(s) 119, 301, 700
Lsp1109I GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
LweI GCATC 4 cut(s) 521, 798, 862, 1362
MaeI CTAG 4 cut(s) 347, 396, 782, 1130
MaeII ACGT 1 cut(s) 677
MalI GATC 5 cut(s) 26, 662, 849, 975, 1326
MboI GATC 5 cut(s) 24, 660, 847, 973, 1324
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 3 cut(s) 124, 224, 549
MlyI GAGTC 3 cut(s) 2, 473, 864
MmeI TCCRAC 1 cut(s) 1310
Mph1103I ATGCAT 1 cut(s) 1166
MroXI GAANNNNTTC 3 cut(s) 688, 955, 1177
MseI TTAA 2 cut(s) 276, 1188
MslI CAYNNNNRTG 2 cut(s) 1320, 1358
MspA1I CMGCKG 2 cut(s) 614, 1330
MspR9I CCNGG 4 cut(s) 155, 226, 245, 1404
MvaI CCWGG 4 cut(s) 155, 226, 245, 1404
MvnI CGCG 5 cut(s) 91, 199, 237, 1255, 1257
MwoI GCNNNNNNNGC 3 cut(s) 191, 467, 1385
NdeII GATC 5 cut(s) 24, 660, 847, 973, 1324
NlaIII CATG 6 cut(s) 737, 854, 940, 1149, 1168, 1431
NlaIV GGNNCC 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 1166
NspI RCATGY 1 cut(s) 1168
PagI TCATGA 1 cut(s) 1427
PauI GCGCGC 1 cut(s) 1253
PceI AGGCCT 2 cut(s) 101, 561
PciSI GCTCTTC 1 cut(s) 212
PdmI GAANNNNTTC 3 cut(s) 688, 955, 1177
PfeI GAWTC 2 cut(s) 291, 1114
PkrI GCNGC 8 cut(s) 160, 184, 196, 239, 616, 1206, 1209, 1332
PleI GAGTC 3 cut(s) 2, 472, 863
PpsI GAGTC 3 cut(s) 2, 472, 863
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 4 cut(s) 153, 224, 243, 1402
PspGI CCWGG 4 cut(s) 153, 224, 243, 1402
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 2 cut(s) 213, 392
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 2 cut(s) 467, 1333
PsuI RGATCY 1 cut(s) 24
PteI GCGCGC 1 cut(s) 1253
PvuII CAGCTG 2 cut(s) 614, 1330
RsaI GTAC 5 cut(s) 441, 696, 771, 1011, 1106
RsaNI GTAC 5 cut(s) 440, 695, 770, 1010, 1105
RseI CAYNNNNRTG 2 cut(s) 1320, 1358
SacI GAGCTC 1 cut(s) 549
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 276, 1188
SatI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
Sau3AI GATC 5 cut(s) 24, 660, 847, 973, 1324
Sau96I GGNCC 2 cut(s) 213, 392
SchI GAGTC 3 cut(s) 2, 473, 864
ScrFI CCNGG 4 cut(s) 155, 226, 245, 1404
SduI GDGCHC 3 cut(s) 124, 224, 549
SfaNI GCATC 4 cut(s) 521, 798, 862, 1362
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 392
SmiMI CAYNNNNRTG 2 cut(s) 1320, 1358
SmlI CTYRAG 4 cut(s) 631, 704, 825, 840
SmoI CTYRAG 4 cut(s) 631, 704, 825, 840
SseBI AGGCCT 2 cut(s) 101, 561
SsiI CCGC 2 cut(s) 199, 237
SspMI CTAG 4 cut(s) 347, 396, 782, 1130
SstI GAGCTC 1 cut(s) 549
StuI AGGCCT 2 cut(s) 101, 561
StyD4I CCNGG 4 cut(s) 153, 224, 243, 1402
TaaI ACNGT 5 cut(s) 273, 331, 895, 989, 1322
TaiI ACGT 1 cut(s) 680
TaqI TCGA 3 cut(s) 315, 335, 549
TaqII GACCGA 2 cut(s) 380, 1313
TatI WGTACW 3 cut(s) 439, 694, 1104
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 291, 1114
Tru1I TTAA 2 cut(s) 276, 1188
Tru9I TTAA 2 cut(s) 276, 1188
TscAI CASTG 1 cut(s) 168
TseI GCWGC 7 cut(s) 158, 182, 194, 614, 1204, 1207, 1330
TspDTI ATGAA 7 cut(s) 393, 527, 851, 1039, 1134, 1244, 1416
TspRI CASTG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 3 cut(s) 386, 684, 930
XceI RCATGY 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 3 cut(s) 688, 955, 1177
XspI CTAG 4 cut(s) 347, 396, 782, 1130
Zsp2I ATGCAT 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.