Prupe.6G080900_v2.0.a1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011426
Physical Location & Seq
Forward (+)
5489716 .. 5493956
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G080900.1

Sequence Viewer

Length: 1437 bp
ATGGGAGACACTTTGCGTAGAATCCCTCAAGAGGCCAATGTTGCTATGACTGGAGGAAAAAATAAGTCACATGATCATATAGTTGCTTCTCGCCCCAACGCCTCTATGCAGCACATAAATTCGCTTCGTATCGGGAATGGAGCTGCGAGCCAGGCGGCATTATTTTTGCTCAAAATGGCCGCACTGGAGACAGTAAGGAGGTTCTCAAGGGCCAAGTGCCCTTGGGCATGGCGTGGCTTTCAGGCTTTGCAATTTCTGTGCTATCCACCGTTCAAGTGGTTTCAACGATTTGCCCCCTTCAAGGCTTTGGTCAATGGCATGCAGGTAGTTTCGAAACCATTACTAGTGCTCTCCATTGCAACGGCTTTCTCTGATGAAGCTGAGCCCTTTAATAGAACCTCTGATGGCATCAACAATTCCGATGCATGTTCAGAAGTGGACTCAGAAGTATCCTCTGAGTGTATCAACGTTTCTACTTTGGATACAAGGATTCCTGATGAATTTCCTCAAAATGTACAACCTGAAATGTGGTTGACACAACTCCACAAAGAGCTGGAAACACAAGGGATTACATTGCCAGAAAGAATTGATGAGGAGGAACTTCGCAGGTTTTACACTGCTGCAAACGGAGACTTCTCTTGCTTGCTATCTTCAATAAAGAAGACGATCAATTGGAGGAAGACCTATGGAATTCTTTCTGTGCAGGAGCTTGAGATGTGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTCATTGTACGACTTGGGCTAGCTTGCACCAGCTTGCCATCTCATGATAGACCACGCTTTGCTCAAGCAATCATTTCTCAAGTAGAGCACGGTGTCTTTCATTTGCTTGATGCCAACAATGCTCAAATTACAGTTGTGGTAGATTGTGAAGGTCTATCTCCATTGAAAATTCCCATGCAAGTTATGAGGACTTGTTCTTCCCTTTTGCAAGATCACTTCCCCAACCGTCTTGGCTGTCTGTTTGTTATACAGCTTCCTCCAGTGCTTCGTGTTATTGCTCAAACTTTTATTCAAGTTTTGAAACCTTACACCCGAGAGAAGTTGAGAATTGAAGGCGAGATGTACCGGAAGATTCTATCTGAGTACCTTGAGACACTTCCATCATATCTTGGTGGCAAATGCATGTGCAAAATATGTTCAGATATTAGCATCACAGATATGCAGCAGCCTCACGCAAATGAAGTGGCCATGACAGAGCAGAGACCGAATGTGCTTGAGGGTGGGAATCCAGTCTCAGCCCATCCAACTTATGAGGCTGAGATTGACATGGACCGCAATTGTGATCAGGTGCTGAGAACTGCGATAGTTGGCATCCTTATGATTTGGGTTTTCATAGCATTTATTGCTGGATTTATCGACCCTGAAAGCCGTCCCATTTTTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

479

Amino Acids

54.02

Weight (kDa)

6.47

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 313, 597
AciI CCGC 3 cut(s) 155, 180, 1321
AclI AACGTT 1 cut(s) 468
AcoI YGGCCR 2 cut(s) 177, 1233
AcsI RAATTY 4 cut(s) 118, 500, 690, 936
AfaI GTAC 4 cut(s) 516, 777, 1112, 1133
AfiI CCNNNNNNNGG 2 cut(s) 31, 301
AgsI TTSAA 8 cut(s) 274, 284, 301, 654, 934, 1061, 1069, 1100
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 7 cut(s) 143, 380, 553, 709, 791, 801, 1021
AluI AGCT 7 cut(s) 143, 380, 553, 709, 791, 801, 1021
Alw21I GWGCWC 2 cut(s) 351, 858
Alw26I GTCTC 5 cut(s) 182, 624, 1133, 1243, 1285
AlwNI CAGNNNCTG 1 cut(s) 1339
Ama87I CYCGRG 1 cut(s) 1080
AoxI GGCC 4 cut(s) 33, 177, 210, 1233
ApeKI GCWGC 5 cut(s) 109, 143, 620, 1210, 1213
ApoI RAATTY 4 cut(s) 118, 500, 690, 936
Asp700I GAANNNNTTC 1 cut(s) 694
AspS9I GGNCC 2 cut(s) 210, 1318
AsuII TTCGAA 1 cut(s) 332
AsuNHI GCTAGC 1 cut(s) 787
AvaI CYCGRG 1 cut(s) 1080
AvaII GGWCC 1 cut(s) 1318
BaeGI GKGCMC 1 cut(s) 221
BalI TGGCCA 1 cut(s) 1235
BanII GRGCYC 1 cut(s) 387
BbsI GAAGAC 2 cut(s) 668, 686
Bbv12I GWGCWC 2 cut(s) 351, 858
BbvI GCAGC 5 cut(s) 121, 130, 607, 1222, 1225
BccI CCATC 4 cut(s) 398, 814, 1156, 1296
BceAI ACGGC 2 cut(s) 378, 1401
BciT130I CCWGG 1 cut(s) 152
BciVI GTATCC 2 cut(s) 460, 475
BclI TGATCA 2 cut(s) 73, 1330
BcoDI GTCTC 5 cut(s) 182, 624, 1133, 1243, 1285
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 2 cut(s) 344, 788
BfuAI ACCTGC 2 cut(s) 313, 597
BfuI GTATCC 2 cut(s) 460, 475
BisI GCNGC 7 cut(s) 110, 144, 156, 180, 621, 1211, 1214
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 7 cut(s) 111, 145, 157, 181, 622, 1212, 1215
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 1318
BmeT110I CYCGRG 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 210, 1318
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 5 cut(s) 412, 417, 868, 1206, 1368
BmtI GCTAGC 1 cut(s) 791
BpiI GAAGAC 2 cut(s) 668, 686
BpmI CTGGAG 3 cut(s) 72, 206, 1011
Bpu1102I GCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 7 cut(s) 12, 190, 731, 816, 831, 1157, 1283
BsaI GGTCTC 1 cut(s) 1243
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 1113
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 301
Bse1I ACTGG 4 cut(s) 55, 189, 1028, 1277
Bse3DI GCAATG 2 cut(s) 354, 572
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 2 cut(s) 1288, 1359
BseLI CCNNNNNNNGG 2 cut(s) 31, 301
BseMI GCAATG 2 cut(s) 354, 572
BseMII CTCAG 7 cut(s) 372, 447, 456, 1119, 1296, 1296, 1331
BseNI ACTGG 4 cut(s) 55, 189, 1028, 1277
BseRI GAGGAG 1 cut(s) 608
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 5 cut(s) 121, 130, 607, 1222, 1225
BsgI GTGCAG 1 cut(s) 722
BshFI GGCC 4 cut(s) 35, 179, 212, 1235
BsiHKAI GWGCWC 2 cut(s) 351, 858
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 1 cut(s) 1114
BslFI GGGAC 1 cut(s) 1404
BslI CCNNNNNNNGG 2 cut(s) 31, 301
BsmAI GTCTC 5 cut(s) 182, 624, 1133, 1243, 1285
BsmFI GGGAC 1 cut(s) 1404
BsnI GGCC 4 cut(s) 35, 179, 212, 1235
Bso31I GGTCTC 1 cut(s) 1243
BsoBI CYCGRG 1 cut(s) 1080
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 4 cut(s) 221, 351, 387, 858
Bsp1407I TGTACA 1 cut(s) 514
Bsp143I GATC 4 cut(s) 73, 666, 979, 1330
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 3 cut(s) 155, 180, 1321
BspANI GGCC 4 cut(s) 35, 179, 212, 1235
BspCNI CTCAG 7 cut(s) 373, 448, 455, 1120, 1295, 1297, 1332
BspHI TCATGA 1 cut(s) 811
BspMI ACCTGC 2 cut(s) 313, 597
BspOI GCTAGC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 1243
BsrDI GCAATG 2 cut(s) 354, 572
BsrGI TGTACA 1 cut(s) 514
BsrI ACTGG 4 cut(s) 55, 189, 1028, 1277
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 4 cut(s) 73, 666, 979, 1330
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 5 cut(s) 193, 270, 860, 901, 995
BstAPI GCANNNNNTGC 1 cut(s) 1391
BstAUI TGTACA 1 cut(s) 514
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 6 cut(s) 148, 320, 644, 789, 793, 803
BstDEI CTNAG 7 cut(s) 381, 442, 456, 1128, 1282, 1305, 1340
BstF5I GGATG 2 cut(s) 1288, 1359
BstKTI GATC 4 cut(s) 76, 669, 982, 1333
BstMAI GTCTC 5 cut(s) 182, 624, 1133, 1243, 1285
BstMBI GATC 4 cut(s) 73, 666, 979, 1330
BstMWI GCNNNNNNNGC 4 cut(s) 41, 152, 887, 1391
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 3 cut(s) 322, 429, 1174
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 221
BstV1I GCAGC 5 cut(s) 121, 130, 607, 1222, 1225
BstV2I GAAGAC 2 cut(s) 668, 686
BsuI GTATCC 2 cut(s) 460, 475
BsuRI GGCC 4 cut(s) 35, 179, 212, 1235
BtsCI GGATG 2 cut(s) 1288, 1359
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 182, 615, 1035
BveI ACCTGC 2 cut(s) 313, 597
Cac8I GCNNGC 6 cut(s) 148, 320, 644, 789, 793, 803
CaiI CAGNNNCTG 1 cut(s) 1339
CciI TCATGA 1 cut(s) 811
Cfr13I GGNCC 2 cut(s) 210, 1318
Csp6I GTAC 4 cut(s) 515, 776, 1111, 1132
CspCI CAANNNNNGTGG 2 cut(s) 1212, 1247
CviQI GTAC 4 cut(s) 515, 776, 1111, 1132
DdeI CTNAG 7 cut(s) 381, 442, 456, 1128, 1282, 1305, 1340
DpnI GATC 4 cut(s) 75, 668, 981, 1332
DpnII GATC 4 cut(s) 73, 666, 979, 1330
EaeI YGGCCR 2 cut(s) 177, 1233
Eco130I CCWWGG 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 1243
Eco47I GGWCC 1 cut(s) 1318
Eco88I CYCGRG 1 cut(s) 1080
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 221
EcoT22I ATGCAT 2 cut(s) 427, 1172
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 221
FaqI GGGAC 1 cut(s) 1404
FbaI TGATCA 2 cut(s) 73, 1330
Fnu4HI GCNGC 7 cut(s) 110, 144, 156, 180, 621, 1211, 1214
FokI GGATG 2 cut(s) 1275, 1346
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 7 cut(s) 110, 144, 156, 180, 621, 1211, 1214
FspBI CTAG 2 cut(s) 344, 788
GluI GCNGC 7 cut(s) 110, 144, 156, 180, 621, 1211, 1214
GsuI CTGGAG 3 cut(s) 72, 206, 1011
HaeIII GGCC 4 cut(s) 35, 179, 212, 1235
HapII CCGG 1 cut(s) 1114
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HinfI GANTC 5 cut(s) 21, 440, 490, 1120, 1273
HpaII CCGG 1 cut(s) 1114
Hpy166II GTNNAC 2 cut(s) 439, 534
Hpy188I TCNGA 8 cut(s) 373, 403, 421, 433, 445, 457, 1129, 1189
Hpy188III TCNNGA 4 cut(s) 29, 133, 494, 812
Hpy8I GTNNAC 2 cut(s) 439, 534
HpyAV CCTTC 3 cut(s) 307, 911, 1094
HpyCH4III ACNGT 5 cut(s) 193, 270, 860, 901, 995
HpyCH4IV ACGT 1 cut(s) 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 152, 887, 1391
HpyF3I CTNAG 7 cut(s) 381, 442, 456, 1128, 1282, 1305, 1340
HpySE526I ACGT 1 cut(s) 468
Ksp22I TGATCA 2 cut(s) 73, 1330
Kzo9I GATC 4 cut(s) 73, 666, 979, 1330
LmnI GCTCC 2 cut(s) 140, 706
Lsp1109I GCAGC 5 cut(s) 121, 130, 607, 1222, 1225
LweI GCATC 5 cut(s) 412, 417, 868, 1206, 1368
MaeI CTAG 2 cut(s) 344, 788
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 66
MalI GATC 4 cut(s) 75, 668, 981, 1332
MboI GATC 4 cut(s) 73, 666, 979, 1330
MboII GAAGA 5 cut(s) 642, 673, 691, 957, 1129
MfeI CAATTG 2 cut(s) 670, 1324
MhlI GDGCHC 4 cut(s) 221, 351, 387, 858
MlsI TGGCCA 1 cut(s) 1235
MluNI TGGCCA 1 cut(s) 1235
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 1 cut(s) 1316
Mox20I TGGCCA 1 cut(s) 1235
Mph1103I ATGCAT 2 cut(s) 427, 1172
MroXI GAANNNNTTC 1 cut(s) 694
MscI TGGCCA 1 cut(s) 1235
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 3 cut(s) 1205, 1224, 1364
Msp20I TGGCCA 1 cut(s) 1235
MspI CCGG 1 cut(s) 1114
MspR9I CCNGG 1 cut(s) 152
MunI CAATTG 2 cut(s) 670, 1324
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 4 cut(s) 41, 152, 887, 1391
NdeII GATC 4 cut(s) 73, 666, 979, 1330
NheI GCTAGC 1 cut(s) 787
NmuCI GTSAC 1 cut(s) 66
NsiI ATGCAT 2 cut(s) 427, 1172
NspI RCATGY 3 cut(s) 322, 429, 1174
NspV TTCGAA 1 cut(s) 332
PaeI GCATGC 1 cut(s) 322
PagI TCATGA 1 cut(s) 811
PdmI GAANNNNTTC 1 cut(s) 694
PfeI GAWTC 4 cut(s) 21, 490, 1120, 1273
PkrI GCNGC 7 cut(s) 111, 145, 157, 181, 622, 1212, 1215
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Psp1406I AACGTT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspPI GGNCC 2 cut(s) 210, 1318
PstNI CAGNNNCTG 1 cut(s) 1339
RsaI GTAC 4 cut(s) 516, 777, 1112, 1133
RsaNI GTAC 4 cut(s) 515, 776, 1111, 1132
RseI CAYNNNNRTG 3 cut(s) 1205, 1224, 1364
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 7 cut(s) 110, 144, 156, 180, 621, 1211, 1214
Sau3AI GATC 4 cut(s) 73, 666, 979, 1330
Sau96I GGNCC 2 cut(s) 210, 1318
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 4 cut(s) 221, 351, 387, 858
SfaNI GCATC 5 cut(s) 412, 417, 868, 1206, 1368
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 1 cut(s) 1318
SmiMI CAYNNNNRTG 3 cut(s) 1205, 1224, 1364
SmlI CTYRAG 7 cut(s) 27, 205, 710, 831, 846, 1136, 1262
SmoI CTYRAG 7 cut(s) 27, 205, 710, 831, 846, 1136, 1262
SpeI ACTAGT 1 cut(s) 343
SphI GCATGC 1 cut(s) 322
SsiI CCGC 3 cut(s) 155, 180, 1321
SspMI CTAG 2 cut(s) 344, 788
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 5 cut(s) 193, 270, 860, 901, 995
TaiI ACGT 1 cut(s) 471
TaqI TCGA 2 cut(s) 332, 1404
TaqII GACCGA 1 cut(s) 1267
TatI WGTACW 1 cut(s) 514
TauI GCSGC 2 cut(s) 158, 182
TfiI GAWTC 4 cut(s) 21, 490, 1120, 1273
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 3 cut(s) 189, 622, 1035
TseFI GTSAC 1 cut(s) 66
TseI GCWGC 5 cut(s) 109, 143, 620, 1210, 1213
Tsp45I GTSAC 1 cut(s) 66
TspDTI ATGAA 5 cut(s) 390, 513, 857, 1242, 1369
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 3 cut(s) 189, 622, 1035
VpaK11BI GGWCC 1 cut(s) 1318
XapI RAATTY 4 cut(s) 118, 500, 690, 936
XceI RCATGY 3 cut(s) 322, 429, 1174
XcmI CCANNNNNNNNNTGG 1 cut(s) 273
XmnI GAANNNNTTC 1 cut(s) 694
XspI CTAG 2 cut(s) 344, 788
Zsp2I ATGCAT 2 cut(s) 427, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.