Rh5DG447300

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011426
Physical Location & Seq
Reverse (-)
71339666 .. 71342998
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG447300.1

Sequence Viewer

Length: 1431 bp
ATGGGAGACTCGTCTTCGTATGGGATCCCTAAAGAGGCCAAGCTCGTTATGTCTGGGAAAAAGCAGTCTCCGAATTACTTAGTTGCTTCGCGCTCTAAGGCCTCTTTGCAAAGCTTGGGAGCACTTGGAATTGGTAATAATGGTGTGGTCAGCCAGGCAGCAGTGTTTCTGCTCAAAGTGGCTGCATTGGAGGCAGCGCGGAGGTTCTCCAGGGCCAAGTGCCCCTGGCTGTGGCGCGGCTTCCAGGCTTTGCAATTTCTGTGTTATCCACCGTTTAAGTGGCTTCGGAGATTCGCTCCCTTCAAGGCTCTGGTCGACAACTTGCAGACGGTATCGAAGCCATTACTAGCCCTCTCCATTGCAACGGCTTTTACTGATGAATTAGAATTCGGTCCTAGAACTTCAGATGATGTCAACAATTCTGAGGCGCGTCCAGAAGTACACCCAGAAGTATCCTCTGCAGAGTCTGCTTTGGATACAAGCGTTTCTGAAGAAGTTCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTTCATAACGAGCTTGAAAGACAAGGGCTTAGCTTGCCAGAAAGAATTGACGAGGAGGAACTTCACAGGTTCTACACAGCTGCTAACAGAGACTTCTCGAGCTTTCTGTCTGCAATAAAGAAGACAATCAGGTGGAGAGAGACTTATGGAATTCTTTCGGTCCAGGAGCTTGAGAAATGGTCAAATATGGTTTTCTGGCATGGATTTGATATGAAACACCGACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTCGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCCCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACTTGTTCTTCGCTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCACAAACTTTTATCCAAGTTTTGAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAATATGTACCGGAAGATTCTCTCTGAGTGCCTAGAGACACTCCCATCATATCTTGGTGGCAAATGCCTCTGCAGAATATGTTCAGATATTAACATGAAAGATATGCAGCAGCCTCGCACAAATGATATTATGAAGAGAGAGCTGAGAGCAAGTGTTTGCGAGGGTGGGAGTTTACACTCTCCCGATCCAACTTGTGAGACCGAGGTTGAACCGAACCATAACTGTGATCAGCTGCTGAGAACTGCTATAGTGAGCATCCTTATGTTTTGGATTTTAATAGCTTTTATTGCGGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

476

Amino Acids

53.54

Weight (kDa)

8.15

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 239 - 386 1.9e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AccII CGCG 4 cut(s) 91, 199, 237, 430
AciI CCGC 3 cut(s) 199, 237, 1388
AclWI GGATC 3 cut(s) 19, 32, 1277
AcsI RAATTY 3 cut(s) 386, 684, 930
AcuI CTGAAG 2 cut(s) 387, 510
AfaI GTAC 4 cut(s) 441, 771, 1011, 1106
AfiI CCNNNNNNNGG 2 cut(s) 34, 231
AgsI TTSAA 6 cut(s) 304, 551, 928, 1063, 1094, 1307
AjnI CCWGG 6 cut(s) 153, 209, 224, 243, 696, 1402
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
AlwI GGATC 3 cut(s) 19, 32, 1277
AlwNI CAGNNNCTG 2 cut(s) 467, 1333
Ama87I CYCGRG 2 cut(s) 631, 1074
AoxI GGCC 3 cut(s) 36, 99, 213
ApeKI GCWGC 7 cut(s) 158, 182, 194, 614, 1204, 1207, 1330
ApoI RAATTY 3 cut(s) 386, 684, 930
Asp700I GAANNNNTTC 4 cut(s) 495, 688, 955, 1177
AspLEI GCGC 4 cut(s) 93, 199, 237, 430
AspS9I GGNCC 3 cut(s) 213, 392, 694
AvaI CYCGRG 2 cut(s) 631, 1074
AvaII GGWCC 2 cut(s) 392, 694
BaeGI GKGCMC 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BamHI GGATCC 1 cut(s) 24
BbsI GAAGAC 2 cut(s) 6, 662
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
BccI CCATC 3 cut(s) 516, 808, 1150
BceAI ACGGC 3 cut(s) 381, 788, 1028
BcgI CGANNNNNNTGC 2 cut(s) 1193, 1227
BciT130I CCWGG 6 cut(s) 155, 211, 226, 245, 698, 1404
BciVI GTATCC 2 cut(s) 463, 469
BclI TGATCA 2 cut(s) 847, 1324
BcoDI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BfaI CTAG 4 cut(s) 347, 396, 782, 1130
BfmI CTRYAG 3 cut(s) 459, 1168, 1344
BfuI GTATCC 2 cut(s) 463, 469
BisI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 8 cut(s) 160, 184, 196, 239, 616, 1206, 1209, 1332
Bme1390I CCNGG 6 cut(s) 155, 211, 226, 245, 698, 1404
Bme18I GGWCC 2 cut(s) 392, 694
BmeT110I CYCGRG 2 cut(s) 631, 1074
BmgT120I GGNCC 3 cut(s) 213, 392, 694
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 6 cut(s) 155, 211, 226, 245, 698, 1404
BmsI GCATC 4 cut(s) 521, 798, 862, 1362
BpiI GAAGAC 2 cut(s) 6, 662
BplI GAGNNNNNCTC 2 cut(s) 280, 312
BpmI CTGGAG 1 cut(s) 193
Bpu1102I GCTNAGC 1 cut(s) 563
BpuEI CTTGAG 3 cut(s) 725, 810, 825
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 4 cut(s) 210, 224, 1299, 1402
BsaWI WCCGGW 1 cut(s) 1107
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 231
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 6 cut(s) 155, 211, 226, 245, 698, 1404
BseDI CCNNGG 4 cut(s) 210, 224, 1299, 1402
BseGI GGATG 1 cut(s) 1353
BseLI CCNNNNNNNGG 2 cut(s) 34, 231
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 4 cut(s) 414, 1113, 1232, 1325
BseRI GAGGAG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
Bsh1236I CGCG 4 cut(s) 91, 199, 237, 430
BshFI GGCC 3 cut(s) 38, 101, 215
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 2 cut(s) 631, 1074
BsiSI CCGG 1 cut(s) 1108
BslFI GGGAC 1 cut(s) 1398
BslI CCNNNNNNNGG 2 cut(s) 34, 231
BsmAI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BsmFI GGGAC 1 cut(s) 1398
BsnI GGCC 3 cut(s) 38, 101, 215
Bso31I GGTCTC 1 cut(s) 1289
BsoBI CYCGRG 2 cut(s) 631, 1074
Bsp1286I GDGCHC 2 cut(s) 124, 224
Bsp143I GATC 5 cut(s) 24, 847, 973, 1282, 1324
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 3 cut(s) 199, 237, 1388
BspANI GGCC 3 cut(s) 38, 101, 215
BspCNI CTCAG 4 cut(s) 415, 1114, 1233, 1326
BspFNI CGCG 4 cut(s) 91, 199, 237, 430
BspHI TCATGA 1 cut(s) 1427
BspLI GGNNCC 1 cut(s) 26
BspMAI CTGCAG 2 cut(s) 463, 1172
BspPI GGATC 3 cut(s) 19, 32, 1277
BspTNI GGTCTC 1 cut(s) 1289
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 210, 224, 1299, 1402
BssMI GATC 5 cut(s) 24, 847, 973, 1282, 1324
Bst2UI CCWGG 6 cut(s) 155, 211, 226, 245, 698, 1404
Bst4CI ACNGT 5 cut(s) 273, 331, 895, 989, 1322
Bst6I CTCTTC 1 cut(s) 1226
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 3 cut(s) 569, 787, 797
BstDEI CTNAG 7 cut(s) 79, 96, 423, 563, 1122, 1241, 1334
BstF5I GGATG 1 cut(s) 1353
BstFNI CGCG 4 cut(s) 91, 199, 237, 430
BstHHI GCGC 4 cut(s) 93, 199, 237, 430
BstKTI GATC 5 cut(s) 27, 850, 976, 1285, 1327
BstMAI GTCTC 5 cut(s) 72, 618, 668, 1127, 1289
BstMBI GATC 5 cut(s) 24, 847, 973, 1282, 1324
BstMWI GCNNNNNNNGC 4 cut(s) 191, 467, 568, 1385
BstNI CCWGG 6 cut(s) 155, 211, 226, 245, 698, 1404
BstSCI CCNGG 6 cut(s) 153, 209, 224, 243, 696, 1402
BstSFI CTRYAG 3 cut(s) 459, 1168, 1344
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 4 cut(s) 91, 199, 237, 430
BstV1I GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
BstV2I GAAGAC 2 cut(s) 6, 662
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 2 cut(s) 463, 469
BsuRI GGCC 3 cut(s) 38, 101, 215
BtsCI GGATG 1 cut(s) 1353
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 3 cut(s) 569, 787, 797
CaiI CAGNNNCTG 2 cut(s) 467, 1333
CciI TCATGA 1 cut(s) 1427
CfoI GCGC 4 cut(s) 93, 199, 237, 430
Cfr13I GGNCC 3 cut(s) 213, 392, 694
CseI GACGC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 440, 770, 1010, 1105
CviAII CATG 5 cut(s) 734, 851, 937, 1192, 1428
CviQI GTAC 4 cut(s) 440, 770, 1010, 1105
DdeI CTNAG 7 cut(s) 79, 96, 423, 563, 1122, 1241, 1334
DpnI GATC 5 cut(s) 26, 849, 975, 1284, 1326
DpnII GATC 5 cut(s) 24, 847, 973, 1282, 1324
Eam1104I CTCTTC 1 cut(s) 1226
EarI CTCTTC 1 cut(s) 1226
Eco147I AGGCCT 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 2 cut(s) 392, 694
Eco57I CTGAAG 2 cut(s) 387, 510
Eco88I CYCGRG 2 cut(s) 631, 1074
EcoRI GAATTC 2 cut(s) 386, 684
EcoRII CCWGG 6 cut(s) 153, 209, 224, 243, 696, 1402
FaeI CATG 5 cut(s) 737, 854, 940, 1195, 1431
FaqI GGGAC 1 cut(s) 1398
FatI CATG 5 cut(s) 733, 850, 936, 1191, 1427
FauI CCCGC 1 cut(s) 1381
FbaI TGATCA 2 cut(s) 847, 1324
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
FokI GGATG 1 cut(s) 1340
Fsp4HI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
FspBI CTAG 4 cut(s) 347, 396, 782, 1130
GlaI GCGC 4 cut(s) 92, 198, 236, 429
GluI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 3 cut(s) 38, 101, 215
HapII CCGG 1 cut(s) 1108
HgaI GACGC 1 cut(s) 419
HhaI GCGC 4 cut(s) 93, 199, 237, 430
Hin1II CATG 5 cut(s) 737, 854, 940, 1195, 1431
Hin6I GCGC 4 cut(s) 91, 197, 235, 428
HinP1I GCGC 4 cut(s) 91, 197, 235, 428
HincII GTYRAC 2 cut(s) 316, 415
HindII GTYRAC 2 cut(s) 316, 415
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 5 cut(s) 8, 291, 464, 855, 1114
HpaII CCGG 1 cut(s) 1108
Hpy166II GTNNAC 4 cut(s) 316, 415, 442, 1271
Hpy188I TCNGA 8 cut(s) 72, 288, 406, 424, 490, 517, 1123, 1183
Hpy188III TCNNGA 5 cut(s) 434, 631, 806, 1280, 1428
Hpy8I GTNNAC 4 cut(s) 316, 415, 442, 1271
HpyAV CCTTC 3 cut(s) 310, 905, 1088
HpyCH4III ACNGT 5 cut(s) 273, 331, 895, 989, 1322
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 467, 568, 1385
HpyF3I CTNAG 7 cut(s) 79, 96, 423, 563, 1122, 1241, 1334
Hsp92II CATG 5 cut(s) 737, 854, 940, 1195, 1431
HspAI GCGC 4 cut(s) 91, 197, 235, 428
Ksp22I TGATCA 2 cut(s) 847, 1324
Kzo9I GATC 5 cut(s) 24, 847, 973, 1282, 1324
LmnI GCTCC 3 cut(s) 119, 301, 700
Lsp1109I GCAGC 7 cut(s) 169, 170, 206, 601, 1216, 1219, 1317
LweI GCATC 4 cut(s) 521, 798, 862, 1362
MaeI CTAG 4 cut(s) 347, 396, 782, 1130
MalI GATC 5 cut(s) 26, 849, 975, 1284, 1326
MboI GATC 5 cut(s) 24, 847, 973, 1282, 1324
MboII GAAGA 8 cut(s) 6, 503, 667, 951, 1123, 1243, 1413, 1416
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 2 cut(s) 124, 224
MlyI GAGTC 3 cut(s) 2, 473, 864
MmeI TCCRAC 1 cut(s) 1310
MroXI GAANNNNTTC 4 cut(s) 495, 688, 955, 1177
MseI TTAA 3 cut(s) 276, 1188, 1373
MslI CAYNNNNRTG 2 cut(s) 1320, 1358
MspA1I CMGCKG 2 cut(s) 614, 1330
MspI CCGG 1 cut(s) 1108
MspR9I CCNGG 6 cut(s) 155, 211, 226, 245, 698, 1404
MvaI CCWGG 6 cut(s) 155, 211, 226, 245, 698, 1404
MvnI CGCG 4 cut(s) 91, 199, 237, 430
MwoI GCNNNNNNNGC 4 cut(s) 191, 467, 568, 1385
NdeII GATC 5 cut(s) 24, 847, 973, 1282, 1324
NlaIII CATG 5 cut(s) 737, 854, 940, 1195, 1431
NlaIV GGNNCC 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 631
PagI TCATGA 1 cut(s) 1427
PceI AGGCCT 1 cut(s) 101
PdmI GAANNNNTTC 4 cut(s) 495, 688, 955, 1177
PfeI GAWTC 2 cut(s) 291, 1114
PfoI TCCNGGA 1 cut(s) 696
PkrI GCNGC 8 cut(s) 160, 184, 196, 239, 616, 1206, 1209, 1332
PleI GAGTC 3 cut(s) 2, 472, 863
PpsI GAGTC 3 cut(s) 2, 472, 863
Psp6I CCWGG 6 cut(s) 153, 209, 224, 243, 696, 1402
PspGI CCWGG 6 cut(s) 153, 209, 224, 243, 696, 1402
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 3 cut(s) 213, 392, 694
PstI CTGCAG 2 cut(s) 463, 1172
PstNI CAGNNNCTG 2 cut(s) 467, 1333
PsuI RGATCY 1 cut(s) 24
PvuII CAGCTG 2 cut(s) 614, 1330
RsaI GTAC 4 cut(s) 441, 771, 1011, 1106
RsaNI GTAC 4 cut(s) 440, 770, 1010, 1105
RseI CAYNNNNRTG 2 cut(s) 1320, 1358
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 3 cut(s) 276, 1188, 1373
SatI GCNGC 8 cut(s) 159, 183, 195, 238, 615, 1205, 1208, 1331
Sau3AI GATC 5 cut(s) 24, 847, 973, 1282, 1324
Sau96I GGNCC 3 cut(s) 213, 392, 694
SchI GAGTC 3 cut(s) 2, 473, 864
ScrFI CCNGG 6 cut(s) 155, 211, 226, 245, 698, 1404
SduI GDGCHC 2 cut(s) 124, 224
SfaNI GCATC 4 cut(s) 521, 798, 862, 1362
SfcI CTRYAG 3 cut(s) 459, 1168, 1344
Sfr274I CTCGAG 1 cut(s) 631
SinI GGWCC 2 cut(s) 392, 694
SlaI CTCGAG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 1320, 1358
SmlI CTYRAG 4 cut(s) 631, 704, 825, 840
SmoI CTYRAG 4 cut(s) 631, 704, 825, 840
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 3 cut(s) 199, 237, 1388
SspMI CTAG 4 cut(s) 347, 396, 782, 1130
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 6 cut(s) 153, 209, 224, 243, 696, 1402
TaaI ACNGT 5 cut(s) 273, 331, 895, 989, 1322
TaqI TCGA 3 cut(s) 315, 335, 632
TaqII GACCGA 3 cut(s) 380, 682, 1313
TatI WGTACW 1 cut(s) 439
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 291, 1114
Tru1I TTAA 3 cut(s) 276, 1188, 1373
Tru9I TTAA 3 cut(s) 276, 1188, 1373
TscAI CASTG 1 cut(s) 168
TseI GCWGC 7 cut(s) 158, 182, 194, 614, 1204, 1207, 1330
TspDTI ATGAA 7 cut(s) 393, 527, 761, 851, 1208, 1244, 1416
TspRI CASTG 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 392, 694
XapI RAATTY 3 cut(s) 386, 684, 930
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XhoI CTCGAG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 4 cut(s) 495, 688, 955, 1177
XspI CTAG 4 cut(s) 347, 396, 782, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.