RLG00000035613

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011426
Physical Location & Seq
Reverse (-)
69332772 .. 69336386
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035613

Sequence Viewer

Length: 1461 bp
ATGGTGGGTTTAGGAAATCCAAGCCCGCCCAAGATTTCAATCCTGGATCCGCCATTGCCTCAAGAGGCCAATCTTGTTATGTCTGGGAAAAAGCAGTCTCCGAATTACTTAGTTGCTTCGCGGTCTAAGGCCTCTTTGCAAAGCTTGGGAGCACTTGGAATTGGTAATAATGGTGTGGTCAGCCAGGCAGCAGTGTTTCTACTCAAAGTGGCTGCATTGGAGGCAGCTCGGAGGCTCTCGAGGGCGAAGTGCCCCTGGCTGTGGCGCGGCTTCCAGGCTTTGCAATTTATGTGTTATCCACCGTTTAAGTGGCTTCGGAGATTCGCTCCGTTCAAGGCTCTGGTTGACAACTTGCAGACGGTATCGAAGCCATTACTAGCCCTCTCCATTGCAACGGCTTTTACTGATGAATTAGAATTCGGTCCTAGAACTTCAGATGATGTCAACAATTCTGATGCGCGTCCAGAAGTACACCCAGAAGTATCCTCTGCAGAGTCTGCTTTGGATACAAGTGTTTCTGAAGAAGTGCCTCAAAATGTAGCATCTGAAAGGTGGTTGATACAACTTCATAACGAGCTTGAAAGACAAGGGCTTAGCCTGCCAGAAAGAATTGACGAGGAGGAACTTTGCAGGTTCTACACAGCTGCTAACGGAGACTTCTCGAGCTTTCTGTCTGCAATAAAGAAGACAATCAGGTGGAGAGAGACTTATGGAATTCTTTCGGTACAGGAGCTTGAGAAATGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTCGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACCTGTTCTTCATTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACTTTCATCCAAGTTTTGAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAAGATGTACCGGAAGATTCTCTCTGAGTGCCTAGACACACTCCCTTCATGTCTTGGTGGCAAATGCCTGTGCAAAATATGTTCAGAAATTAACACGAAAGATATGCAGCAGCCTCGCACAAATGATATTATGAAGAGAGAGCTGAAAGCGAGTGTTTGCGAGGATGGGAGTTTATGCTCTCTCAGTCCAACTTGTGAGACCGAGGTTGAACCGAACCATTACTGTGATCAGCTGCTGAGGACTGCCATAGTGAGCGTCCTTATGTTTTGGATTTTAGTAGCTTTTATTGCTGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

487

Amino Acids

54.27

Weight (kDa)

7.82

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 249 - 396 5.1e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 621
AccII CGCG 3 cut(s) 121, 267, 460
AciI CCGC 4 cut(s) 26, 50, 121, 267
AclWI GGATC 2 cut(s) 41, 54
AcsI RAATTY 3 cut(s) 416, 714, 960
AcuI CTGAAG 2 cut(s) 417, 540
AfaI GTAC 5 cut(s) 471, 726, 801, 1041, 1136
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 7 cut(s) 39, 334, 581, 958, 1093, 1124, 1337
AjnI CCWGG 5 cut(s) 42, 183, 254, 273, 1432
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 4 cut(s) 102, 648, 698, 1319
AlwI GGATC 2 cut(s) 41, 54
AlwNI CAGNNNCTG 2 cut(s) 497, 1363
Ama87I CYCGRG 3 cut(s) 238, 661, 1104
AoxI GGCC 2 cut(s) 66, 129
ApeKI GCWGC 7 cut(s) 188, 212, 224, 644, 1234, 1237, 1360
ApoI RAATTY 3 cut(s) 416, 714, 960
Asp700I GAANNNNTTC 2 cut(s) 718, 985
AspLEI GCGC 2 cut(s) 267, 460
AspS9I GGNCC 1 cut(s) 422
AvaI CYCGRG 3 cut(s) 238, 661, 1104
AvaII GGWCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 254
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BamHI GGATCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 1364
BbvI GCAGC 7 cut(s) 199, 200, 236, 631, 1246, 1249, 1347
BccI CCATC 2 cut(s) 838, 1286
BceAI ACGGC 3 cut(s) 411, 818, 1058
BcgI CGANNNNNNTGC 4 cut(s) 1213, 1223, 1247, 1257
BciT130I CCWGG 5 cut(s) 44, 185, 256, 275, 1434
BciVI GTATCC 2 cut(s) 493, 499
BclI TGATCA 2 cut(s) 877, 1354
BcoDI GTCTC 4 cut(s) 102, 648, 698, 1319
BfaI CTAG 4 cut(s) 377, 426, 812, 1160
BfmI CTRYAG 1 cut(s) 489
BfuAI ACCTGC 1 cut(s) 621
BfuI GTATCC 2 cut(s) 493, 499
BisI GCNGC 8 cut(s) 189, 213, 225, 268, 645, 1235, 1238, 1361
BlpI GCTNAGC 1 cut(s) 593
BlsI GCNGC 8 cut(s) 190, 214, 226, 269, 646, 1236, 1239, 1362
Bme1390I CCNGG 5 cut(s) 44, 185, 256, 275, 1434
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 3 cut(s) 238, 661, 1104
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 5 cut(s) 44, 185, 256, 275, 1434
BmsI GCATC 4 cut(s) 445, 551, 828, 892
BpiI GAAGAC 1 cut(s) 692
BplI GAGNNNNNCTC 2 cut(s) 310, 342
Bpu10I CCTNAGC 1 cut(s) 1364
Bpu1102I GCTNAGC 1 cut(s) 593
BpuEI CTTGAG 4 cut(s) 45, 755, 840, 855
BsaBI GATNNNNATC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 1319
BsaJI CCNNGG 3 cut(s) 254, 1329, 1432
BsaWI WCCGGW 1 cut(s) 1137
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse3DI GCAATG 2 cut(s) 53, 387
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 5 cut(s) 44, 185, 256, 275, 1434
BseDI CCNNGG 3 cut(s) 254, 1329, 1432
BseGI GGATG 2 cut(s) 1080, 1297
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMI GCAATG 2 cut(s) 53, 387
BseMII CTCAG 3 cut(s) 1143, 1324, 1355
BseRI GAGGAG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 7 cut(s) 199, 200, 236, 631, 1246, 1249, 1347
Bsh1236I CGCG 3 cut(s) 121, 267, 460
BshFI GGCC 2 cut(s) 68, 131
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 3 cut(s) 238, 661, 1104
BsiSI CCGG 1 cut(s) 1138
BslFI GGGAC 1 cut(s) 1428
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 4 cut(s) 102, 648, 698, 1319
BsmFI GGGAC 1 cut(s) 1428
BsnI GGCC 2 cut(s) 68, 131
Bso31I GGTCTC 1 cut(s) 1319
BsoBI CYCGRG 3 cut(s) 238, 661, 1104
Bsp1286I GDGCHC 2 cut(s) 154, 254
Bsp143I GATC 4 cut(s) 46, 877, 1003, 1354
Bsp1720I GCTNAGC 1 cut(s) 593
BspACI CCGC 4 cut(s) 26, 50, 121, 267
BspANI GGCC 2 cut(s) 68, 131
BspCNI CTCAG 3 cut(s) 1144, 1323, 1356
BspFNI CGCG 3 cut(s) 121, 267, 460
BspHI TCATGA 1 cut(s) 1457
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 2 cut(s) 41, 54
BspTNI GGTCTC 1 cut(s) 1319
BsrDI GCAATG 2 cut(s) 53, 387
BssECI CCNNGG 3 cut(s) 254, 1329, 1432
BssMI GATC 4 cut(s) 46, 877, 1003, 1354
Bst2UI CCWGG 5 cut(s) 44, 185, 256, 275, 1434
Bst4CI ACNGT 5 cut(s) 303, 361, 925, 1019, 1352
Bst6I CTCTTC 1 cut(s) 1256
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 4 cut(s) 26, 599, 817, 827
BstDEI CTNAG 6 cut(s) 109, 126, 593, 1152, 1310, 1364
BstF5I GGATG 2 cut(s) 1080, 1297
BstFNI CGCG 3 cut(s) 121, 267, 460
BstHHI GCGC 2 cut(s) 267, 460
BstKTI GATC 4 cut(s) 49, 880, 1006, 1357
BstMAI GTCTC 4 cut(s) 102, 648, 698, 1319
BstMBI GATC 4 cut(s) 46, 877, 1003, 1354
BstMWI GCNNNNNNNGC 4 cut(s) 221, 497, 598, 1415
BstNI CCWGG 5 cut(s) 44, 185, 256, 275, 1434
BstSCI CCNGG 5 cut(s) 42, 183, 254, 273, 1432
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 3 cut(s) 121, 267, 460
BstV1I GCAGC 7 cut(s) 199, 200, 236, 631, 1246, 1249, 1347
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuI GTATCC 2 cut(s) 493, 499
BsuRI GGCC 2 cut(s) 68, 131
BtsCI GGATG 2 cut(s) 1080, 1297
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 4 cut(s) 26, 599, 817, 827
CaiI CAGNNNCTG 2 cut(s) 497, 1363
CciI TCATGA 1 cut(s) 1457
CfoI GCGC 2 cut(s) 267, 460
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 2 cut(s) 449, 1372
Csp6I GTAC 5 cut(s) 470, 725, 800, 1040, 1135
CviAII CATG 5 cut(s) 764, 881, 967, 1176, 1458
CviQI GTAC 5 cut(s) 470, 725, 800, 1040, 1135
DdeI CTNAG 6 cut(s) 109, 126, 593, 1152, 1310, 1364
DpnI GATC 4 cut(s) 48, 879, 1005, 1356
DpnII GATC 4 cut(s) 46, 877, 1003, 1354
Eam1104I CTCTTC 1 cut(s) 1256
EarI CTCTTC 1 cut(s) 1256
EciI GGCGGA 1 cut(s) 39
Eco147I AGGCCT 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 1319
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 417, 540
Eco88I CYCGRG 3 cut(s) 238, 661, 1104
EcoRI GAATTC 2 cut(s) 416, 714
EcoRII CCWGG 5 cut(s) 42, 183, 254, 273, 1432
FaeI CATG 5 cut(s) 767, 884, 970, 1179, 1461
FaqI GGGAC 1 cut(s) 1428
FatI CATG 5 cut(s) 763, 880, 966, 1175, 1457
FauI CCCGC 1 cut(s) 33
FbaI TGATCA 2 cut(s) 877, 1354
Fnu4HI GCNGC 8 cut(s) 189, 213, 225, 268, 645, 1235, 1238, 1361
FokI GGATG 2 cut(s) 1067, 1304
Fsp4HI GCNGC 8 cut(s) 189, 213, 225, 268, 645, 1235, 1238, 1361
FspBI CTAG 4 cut(s) 377, 426, 812, 1160
GlaI GCGC 2 cut(s) 266, 459
GluI GCNGC 8 cut(s) 189, 213, 225, 268, 645, 1235, 1238, 1361
HaeIII GGCC 2 cut(s) 68, 131
HapII CCGG 1 cut(s) 1138
HgaI GACGC 2 cut(s) 449, 1372
HhaI GCGC 2 cut(s) 267, 460
Hin1II CATG 5 cut(s) 767, 884, 970, 1179, 1461
Hin6I GCGC 2 cut(s) 265, 458
HinP1I GCGC 2 cut(s) 265, 458
HincII GTYRAC 2 cut(s) 346, 445
HindII GTYRAC 2 cut(s) 346, 445
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 4 cut(s) 321, 494, 885, 1144
HpaII CCGG 1 cut(s) 1138
Hpy166II GTNNAC 3 cut(s) 346, 445, 472
Hpy188I TCNGA 9 cut(s) 102, 231, 318, 436, 454, 520, 547, 1153, 1213
Hpy188III TCNNGA 6 cut(s) 62, 238, 464, 661, 836, 1458
Hpy8I GTNNAC 3 cut(s) 346, 445, 472
HpyAV CCTTC 3 cut(s) 935, 1118, 1182
HpyCH4III ACNGT 5 cut(s) 303, 361, 925, 1019, 1352
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 497, 598, 1415
HpyF3I CTNAG 6 cut(s) 109, 126, 593, 1152, 1310, 1364
Hsp92II CATG 5 cut(s) 767, 884, 970, 1179, 1461
HspAI GCGC 2 cut(s) 265, 458
Ksp22I TGATCA 2 cut(s) 877, 1354
Kzo9I GATC 4 cut(s) 46, 877, 1003, 1354
LmnI GCTCC 3 cut(s) 149, 331, 730
Lsp1109I GCAGC 7 cut(s) 199, 200, 236, 631, 1246, 1249, 1347
LweI GCATC 4 cut(s) 445, 551, 828, 892
MaeI CTAG 4 cut(s) 377, 426, 812, 1160
MalI GATC 4 cut(s) 48, 879, 1005, 1356
MboI GATC 4 cut(s) 46, 877, 1003, 1354
MboII GAAGA 8 cut(s) 533, 697, 981, 1141, 1153, 1273, 1443, 1446
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 2 cut(s) 154, 254
MlyI GAGTC 2 cut(s) 503, 894
MmeI TCCRAC 1 cut(s) 1340
MroXI GAANNNNTTC 2 cut(s) 718, 985
MseI TTAA 2 cut(s) 306, 1218
MslI CAYNNNNRTG 1 cut(s) 1350
MspA1I CMGCKG 2 cut(s) 644, 1360
MspI CCGG 1 cut(s) 1138
MspR9I CCNGG 5 cut(s) 44, 185, 256, 275, 1434
MvaI CCWGG 5 cut(s) 44, 185, 256, 275, 1434
MvnI CGCG 3 cut(s) 121, 267, 460
MwoI GCNNNNNNNGC 4 cut(s) 221, 497, 598, 1415
NdeII GATC 4 cut(s) 46, 877, 1003, 1354
NlaIII CATG 5 cut(s) 767, 884, 970, 1179, 1461
NlaIV GGNNCC 1 cut(s) 48
PaeR7I CTCGAG 2 cut(s) 238, 661
PagI TCATGA 1 cut(s) 1457
PceI AGGCCT 1 cut(s) 131
PdmI GAANNNNTTC 2 cut(s) 718, 985
PfeI GAWTC 2 cut(s) 321, 1144
PfoI TCCNGGA 1 cut(s) 42
PkrI GCNGC 8 cut(s) 190, 214, 226, 269, 646, 1236, 1239, 1362
PleI GAGTC 2 cut(s) 502, 893
PpsI GAGTC 2 cut(s) 502, 893
Psp6I CCWGG 5 cut(s) 42, 183, 254, 273, 1432
PspGI CCWGG 5 cut(s) 42, 183, 254, 273, 1432
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 422
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 2 cut(s) 497, 1363
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 2 cut(s) 644, 1360
RsaI GTAC 5 cut(s) 471, 726, 801, 1041, 1136
RsaNI GTAC 5 cut(s) 470, 725, 800, 1040, 1135
RseI CAYNNNNRTG 1 cut(s) 1350
SaqAI TTAA 2 cut(s) 306, 1218
SatI GCNGC 8 cut(s) 189, 213, 225, 268, 645, 1235, 1238, 1361
Sau3AI GATC 4 cut(s) 46, 877, 1003, 1354
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 2 cut(s) 503, 894
ScrFI CCNGG 5 cut(s) 44, 185, 256, 275, 1434
SduI GDGCHC 2 cut(s) 154, 254
SfaNI GCATC 4 cut(s) 445, 551, 828, 892
SfcI CTRYAG 1 cut(s) 489
Sfr274I CTCGAG 2 cut(s) 238, 661
SinI GGWCC 1 cut(s) 422
SlaI CTCGAG 2 cut(s) 238, 661
SmiMI CAYNNNNRTG 1 cut(s) 1350
SmlI CTYRAG 6 cut(s) 60, 238, 661, 734, 855, 870
SmoI CTYRAG 6 cut(s) 60, 238, 661, 734, 855, 870
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 4 cut(s) 26, 50, 121, 267
SspMI CTAG 4 cut(s) 377, 426, 812, 1160
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 5 cut(s) 42, 183, 254, 273, 1432
TaaI ACNGT 5 cut(s) 303, 361, 925, 1019, 1352
TaqI TCGA 3 cut(s) 239, 365, 662
TaqII GACCGA 2 cut(s) 410, 1343
TatI WGTACW 1 cut(s) 469
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 2 cut(s) 321, 1144
Tru1I TTAA 2 cut(s) 306, 1218
Tru9I TTAA 2 cut(s) 306, 1218
TscAI CASTG 1 cut(s) 198
TseI GCWGC 7 cut(s) 188, 212, 224, 644, 1234, 1237, 1360
TspDTI ATGAA 8 cut(s) 423, 557, 881, 981, 1069, 1164, 1274, 1446
TspGWI ACGGA 2 cut(s) 318, 666
TspRI CASTG 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 3 cut(s) 416, 714, 960
XcmI CCANNNNNNNNNTGG 1 cut(s) 306
XhoI CTCGAG 2 cut(s) 238, 661
XmnI GAANNNNTTC 2 cut(s) 718, 985
XspI CTAG 4 cut(s) 377, 426, 812, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.