Rmu_ssc0000309.1_g000026

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000309.1
OG0011426
Physical Location & Seq
Forward (+)
82722 .. 85821
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000309.1_g000026.1.cds

Sequence Viewer

Length: 1305 bp
atgggagactcgtcttcgtatgggatccctaaagaggccaagcttgctatgtctgggaaaaagcagtctccgaattacttagttgcttcgcgctctaaggcctctttgcaaagcttgggagcacttggaattggtaataatggtgtggtcagccaggcagcagtgtttctgctcaaagtggctgcattggaggcagcgcggaggctctcgagggccaagtgcccctggctgtggcgcggcttccaggctttgcaatttctgtgttatccaccgtttaagtggcttcggagattcgctcccttcaaggctctggtcgacaacttgcagacggtatcgaagccattactagccctctccattgcaacggcttttactgatgaattagaattcggtcctagaacttcagatgatgtcaacaattctgaggcgcgtccagaagtacacccagaagtatcctctgcagagtctgctttggatacaagcgtttctgaagaagttcctcaaaatgtagcatctgaaagatggttgatacaacttcataacgagcttgaaagacaagggcttagcttgccagaaagattcgctcttattcccctctttctgcaaggtcagtgtaagatcactgatgtgcataatcaactgaggttaacgttaagtaagggccttgcgattaatgttaaggaagtgcctgtaaattcaagtacttacttttctcaagttgatcatggagtctttcatttacttgatgccaacaattctcaaattacagttgtagtagattgtgaaggtctaactccattgaaaattcccatgcaagtattgagaacctgttcttcgctattgcaagatcactttcccaaccgtcttggctgtctgtttgttgtacggcttcctccaatgcttcgtgttattgcccaaactttcatccaagttttgaaaccttacacccgagaaaagttgagaattgaagggaagatgtaccggaagagtctctctgagtgcctagagacactcccttcatgtcttggtggcaaatgcatgtgcagaatatgttcagaaatcaacacaaaagatatgcagcagcctcacacaaatgatattatgaagagagagctgaaagcgagtgtttgcgaggatgggagtttatgctctcccggtccaacttgtgagaccgaggttgaaccgaaccattactgtgatcagctgctgagaactgccatagtgagcatccttatgttttggattttagtagcttttattgctggattatatgaccctggaagcagtcccttttcttcttcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

434

Amino Acids

48.12

Weight (kDa)

8.12

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AccII CGCG 4 cut(s) 91, 199, 237, 430
AciI CCGC 2 cut(s) 199, 237
AclI AACGTT 1 cut(s) 650
AclWI GGATC 2 cut(s) 19, 32
AcsI RAATTY 3 cut(s) 386, 694, 804
AcuI CTGAAG 2 cut(s) 387, 510
AfaI GTAC 4 cut(s) 441, 703, 885, 980
AfiI CCNNNNNNNGG 2 cut(s) 34, 231
AgsI TTSAA 7 cut(s) 304, 551, 699, 802, 937, 968, 1181
AjnI CCWGG 4 cut(s) 153, 224, 243, 1276
AleI CACNNNNGTG 1 cut(s) 626
AluBI AGCT 7 cut(s) 43, 114, 547, 567, 1114, 1204, 1253
AluI AGCT 7 cut(s) 43, 114, 547, 567, 1114, 1204, 1253
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 4 cut(s) 72, 995, 1001, 1163
AlwI GGATC 2 cut(s) 19, 32
AlwNI CAGNNNCTG 2 cut(s) 467, 1207
Ama87I CYCGRG 2 cut(s) 208, 948
AoxI GGCC 4 cut(s) 36, 99, 213, 661
ApeKI GCWGC 6 cut(s) 158, 182, 194, 1078, 1081, 1204
ApoI RAATTY 3 cut(s) 386, 694, 804
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 3 cut(s) 495, 829, 1051
AspLEI GCGC 4 cut(s) 93, 199, 237, 430
AspS9I GGNCC 4 cut(s) 213, 392, 661, 1157
AsuC2I CCSGG 1 cut(s) 1155
AvaI CYCGRG 2 cut(s) 208, 948
AvaII GGWCC 2 cut(s) 392, 1157
BaeGI GKGCMC 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BamHI GGATCC 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 6 cut(s) 169, 170, 206, 1090, 1093, 1191
BccI CCATC 2 cut(s) 516, 1130
BceAI ACGGC 2 cut(s) 381, 902
BciT130I CCWGG 4 cut(s) 155, 226, 245, 1278
BciVI GTATCC 2 cut(s) 463, 469
BclI TGATCA 2 cut(s) 721, 1198
BcnI CCSGG 1 cut(s) 1155
BcoDI GTCTC 4 cut(s) 72, 995, 1001, 1163
BfaI CTAG 3 cut(s) 347, 396, 1004
BfmI CTRYAG 1 cut(s) 459
BfuI GTATCC 2 cut(s) 463, 469
BisI GCNGC 7 cut(s) 159, 183, 195, 238, 1079, 1082, 1205
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 7 cut(s) 160, 184, 196, 239, 1080, 1083, 1206
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 5 cut(s) 155, 226, 245, 1155, 1278
Bme18I GGWCC 2 cut(s) 392, 1157
BmeT110I CYCGRG 2 cut(s) 208, 948
BmgT120I GGNCC 4 cut(s) 213, 392, 661, 1157
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 5 cut(s) 155, 226, 245, 1155, 1278
BmsI GCATC 3 cut(s) 521, 736, 1236
BpiI GAAGAC 1 cut(s) 6
BplI GAGNNNNNCTC 2 cut(s) 280, 312
Bpu1102I GCTNAGC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 699
BpuMI CCSGG 1 cut(s) 1155
BsaI GGTCTC 1 cut(s) 1163
BsaJI CCNNGG 3 cut(s) 224, 1173, 1276
BsaWI WCCGGW 1 cut(s) 981
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 231
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 4 cut(s) 155, 226, 245, 1278
BseDI CCNNGG 3 cut(s) 224, 1173, 1276
BseGI GGATG 3 cut(s) 924, 1141, 1227
BseLI CCNNNNNNNGG 2 cut(s) 34, 231
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 4 cut(s) 414, 632, 987, 1199
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 6 cut(s) 169, 170, 206, 1090, 1093, 1191
BsgI GTGCAG 1 cut(s) 1063
Bsh1236I CGCG 4 cut(s) 91, 199, 237, 430
BshFI GGCC 4 cut(s) 38, 101, 215, 663
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 2 cut(s) 208, 948
BsiSI CCGG 2 cut(s) 982, 1155
BslFI GGGAC 1 cut(s) 1272
BslI CCNNNNNNNGG 2 cut(s) 34, 231
BsmAI GTCTC 4 cut(s) 72, 995, 1001, 1163
BsmFI GGGAC 1 cut(s) 1272
BsnI GGCC 4 cut(s) 38, 101, 215, 663
Bso31I GGTCTC 1 cut(s) 1163
BsoBI CYCGRG 2 cut(s) 208, 948
Bsp1286I GDGCHC 2 cut(s) 124, 224
Bsp143I GATC 5 cut(s) 24, 618, 721, 847, 1198
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 2 cut(s) 199, 237
BspANI GGCC 4 cut(s) 38, 101, 215, 663
BspCNI CTCAG 4 cut(s) 415, 633, 988, 1200
BspFNI CGCG 4 cut(s) 91, 199, 237, 430
BspHI TCATGA 1 cut(s) 1301
BspLI GGNNCC 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 2 cut(s) 19, 32
BspTNI GGTCTC 1 cut(s) 1163
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 224, 1173, 1276
BssMI GATC 5 cut(s) 24, 618, 721, 847, 1198
Bst2UI CCWGG 4 cut(s) 155, 226, 245, 1278
Bst4CI ACNGT 5 cut(s) 273, 331, 769, 863, 1196
Bst6I CTCTTC 2 cut(s) 980, 1100
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 2 cut(s) 45, 569
BstDEI CTNAG 7 cut(s) 79, 96, 423, 563, 641, 996, 1208
BstF5I GGATG 3 cut(s) 924, 1141, 1227
BstFNI CGCG 4 cut(s) 91, 199, 237, 430
BstHHI GCGC 4 cut(s) 93, 199, 237, 430
BstKTI GATC 5 cut(s) 27, 621, 724, 850, 1201
BstMAI GTCTC 4 cut(s) 72, 995, 1001, 1163
BstMBI GATC 5 cut(s) 24, 618, 721, 847, 1198
BstMWI GCNNNNNNNGC 5 cut(s) 44, 191, 467, 568, 1259
BstNI CCWGG 4 cut(s) 155, 226, 245, 1278
BstNSI RCATGY 1 cut(s) 1042
BstSCI CCNGG 5 cut(s) 153, 224, 243, 1153, 1276
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 4 cut(s) 91, 199, 237, 430
BstV1I GCAGC 6 cut(s) 169, 170, 206, 1090, 1093, 1191
BstV2I GAAGAC 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 2 cut(s) 463, 469
BsuRI GGCC 4 cut(s) 38, 101, 215, 663
BtsCI GGATG 3 cut(s) 924, 1141, 1227
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 3 cut(s) 168, 617, 621
Cac8I GCNNGC 2 cut(s) 45, 569
CaiI CAGNNNCTG 2 cut(s) 467, 1207
CciI TCATGA 1 cut(s) 1301
CfoI GCGC 4 cut(s) 93, 199, 237, 430
Cfr13I GGNCC 4 cut(s) 213, 392, 661, 1157
CseI GACGC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 440, 702, 884, 979
CviAII CATG 5 cut(s) 725, 811, 1020, 1039, 1302
CviQI GTAC 4 cut(s) 440, 702, 884, 979
DdeI CTNAG 7 cut(s) 79, 96, 423, 563, 641, 996, 1208
DpnI GATC 5 cut(s) 26, 620, 723, 849, 1200
DpnII GATC 5 cut(s) 24, 618, 721, 847, 1198
Eam1104I CTCTTC 2 cut(s) 980, 1100
EarI CTCTTC 2 cut(s) 980, 1100
Eco147I AGGCCT 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 1163
Eco47I GGWCC 2 cut(s) 392, 1157
Eco57I CTGAAG 2 cut(s) 387, 510
Eco88I CYCGRG 2 cut(s) 208, 948
EcoO109I RGGNCCY 1 cut(s) 661
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 4 cut(s) 153, 224, 243, 1276
EcoT22I ATGCAT 1 cut(s) 1040
FaeI CATG 5 cut(s) 728, 814, 1023, 1042, 1305
FaqI GGGAC 1 cut(s) 1272
FatI CATG 5 cut(s) 724, 810, 1019, 1038, 1301
FbaI TGATCA 2 cut(s) 721, 1198
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 7 cut(s) 159, 183, 195, 238, 1079, 1082, 1205
FokI GGATG 3 cut(s) 911, 1148, 1214
Fsp4HI GCNGC 7 cut(s) 159, 183, 195, 238, 1079, 1082, 1205
FspBI CTAG 3 cut(s) 347, 396, 1004
GlaI GCGC 4 cut(s) 92, 198, 236, 429
GluI GCNGC 7 cut(s) 159, 183, 195, 238, 1079, 1082, 1205
HaeIII GGCC 4 cut(s) 38, 101, 215, 663
HapII CCGG 2 cut(s) 982, 1155
HgaI GACGC 1 cut(s) 419
HhaI GCGC 4 cut(s) 93, 199, 237, 430
Hin1II CATG 5 cut(s) 728, 814, 1023, 1042, 1305
Hin6I GCGC 4 cut(s) 91, 197, 235, 428
HinP1I GCGC 4 cut(s) 91, 197, 235, 428
HincII GTYRAC 3 cut(s) 316, 415, 648
HindII GTYRAC 3 cut(s) 316, 415, 648
HindIII AAGCTT 2 cut(s) 41, 112
HinfI GANTC 6 cut(s) 8, 291, 464, 579, 729, 988
HpaI GTTAAC 1 cut(s) 648
HpaII CCGG 2 cut(s) 982, 1155
Hpy166II GTNNAC 4 cut(s) 316, 415, 442, 648
Hpy188I TCNGA 8 cut(s) 72, 288, 406, 424, 490, 517, 997, 1057
Hpy188III TCNNGA 3 cut(s) 208, 434, 1302
Hpy8I GTNNAC 4 cut(s) 316, 415, 442, 648
HpyAV CCTTC 4 cut(s) 310, 779, 962, 1026
HpyCH4III ACNGT 5 cut(s) 273, 331, 769, 863, 1196
HpyCH4IV ACGT 1 cut(s) 650
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 191, 467, 568, 1259
HpyF3I CTNAG 7 cut(s) 79, 96, 423, 563, 641, 996, 1208
HpySE526I ACGT 1 cut(s) 650
Hsp92II CATG 5 cut(s) 728, 814, 1023, 1042, 1305
HspAI GCGC 4 cut(s) 91, 197, 235, 428
Ksp22I TGATCA 2 cut(s) 721, 1198
KspAI GTTAAC 1 cut(s) 648
Kzo9I GATC 5 cut(s) 24, 618, 721, 847, 1198
LmnI GCTCC 2 cut(s) 119, 301
Lsp1109I GCAGC 6 cut(s) 169, 170, 206, 1090, 1093, 1191
LweI GCATC 3 cut(s) 521, 736, 1236
MaeI CTAG 3 cut(s) 347, 396, 1004
MaeII ACGT 1 cut(s) 650
MalI GATC 5 cut(s) 26, 620, 723, 849, 1200
MboI GATC 5 cut(s) 24, 618, 721, 847, 1198
MboII GAAGA 8 cut(s) 6, 503, 825, 985, 997, 1117, 1287, 1290
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 2 cut(s) 124, 224
MlyI GAGTC 4 cut(s) 2, 473, 738, 997
MmeI TCCRAC 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 1040
MroXI GAANNNNTTC 3 cut(s) 495, 829, 1051
MseI TTAA 5 cut(s) 276, 647, 653, 672, 678
MslI CAYNNNNRTG 4 cut(s) 626, 1092, 1194, 1232
MspA1I CMGCKG 1 cut(s) 1204
MspI CCGG 2 cut(s) 982, 1155
MspR9I CCNGG 5 cut(s) 155, 226, 245, 1155, 1278
MvaI CCWGG 4 cut(s) 155, 226, 245, 1278
MvnI CGCG 4 cut(s) 91, 199, 237, 430
MwoI GCNNNNNNNGC 5 cut(s) 44, 191, 467, 568, 1259
NciI CCSGG 1 cut(s) 1155
NdeII GATC 5 cut(s) 24, 618, 721, 847, 1198
NlaIII CATG 5 cut(s) 728, 814, 1023, 1042, 1305
NlaIV GGNNCC 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 1040
NspI RCATGY 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 208
PagI TCATGA 1 cut(s) 1301
PceI AGGCCT 1 cut(s) 101
PdmI GAANNNNTTC 3 cut(s) 495, 829, 1051
PfeI GAWTC 2 cut(s) 291, 579
PkrI GCNGC 7 cut(s) 160, 184, 196, 239, 1080, 1083, 1206
PleI GAGTC 4 cut(s) 2, 472, 737, 996
PpsI GAGTC 4 cut(s) 2, 472, 737, 996
PshBI ATTAAT 1 cut(s) 672
Psp1406I AACGTT 1 cut(s) 650
Psp6I CCWGG 4 cut(s) 153, 224, 243, 1276
PspGI CCWGG 4 cut(s) 153, 224, 243, 1276
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 4 cut(s) 213, 392, 661, 1157
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 2 cut(s) 467, 1207
PsuI RGATCY 1 cut(s) 24
PvuII CAGCTG 1 cut(s) 1204
RsaI GTAC 4 cut(s) 441, 703, 885, 980
RsaNI GTAC 4 cut(s) 440, 702, 884, 979
RseI CAYNNNNRTG 4 cut(s) 626, 1092, 1194, 1232
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 5 cut(s) 276, 647, 653, 672, 678
SatI GCNGC 7 cut(s) 159, 183, 195, 238, 1079, 1082, 1205
Sau3AI GATC 5 cut(s) 24, 618, 721, 847, 1198
Sau96I GGNCC 4 cut(s) 213, 392, 661, 1157
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 4 cut(s) 2, 473, 738, 997
ScrFI CCNGG 5 cut(s) 155, 226, 245, 1155, 1278
SduI GDGCHC 2 cut(s) 124, 224
SfaNI GCATC 3 cut(s) 521, 736, 1236
SfcI CTRYAG 1 cut(s) 459
Sfr274I CTCGAG 1 cut(s) 208
SinI GGWCC 2 cut(s) 392, 1157
SlaI CTCGAG 1 cut(s) 208
SmiMI CAYNNNNRTG 4 cut(s) 626, 1092, 1194, 1232
SmlI CTYRAG 2 cut(s) 208, 714
SmoI CTYRAG 2 cut(s) 208, 714
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 2 cut(s) 199, 237
SspMI CTAG 3 cut(s) 347, 396, 1004
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 5 cut(s) 153, 224, 243, 1153, 1276
TaaI ACNGT 5 cut(s) 273, 331, 769, 863, 1196
TaiI ACGT 1 cut(s) 653
TaqI TCGA 3 cut(s) 209, 315, 335
TaqII GACCGA 2 cut(s) 380, 1187
TatI WGTACW 2 cut(s) 439, 701
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 291, 579
Tru1I TTAA 5 cut(s) 276, 647, 653, 672, 678
Tru9I TTAA 5 cut(s) 276, 647, 653, 672, 678
TscAI CASTG 3 cut(s) 168, 617, 628
TseI GCWGC 6 cut(s) 158, 182, 194, 1078, 1081, 1204
TspDTI ATGAA 7 cut(s) 393, 527, 725, 913, 1008, 1118, 1290
TspRI CASTG 3 cut(s) 168, 617, 628
VpaK11BI GGWCC 2 cut(s) 392, 1157
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 3 cut(s) 386, 694, 804
XceI RCATGY 1 cut(s) 1042
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XhoI CTCGAG 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 3 cut(s) 495, 829, 1051
XspI CTAG 3 cut(s) 347, 396, 1004
ZrmI AGTACT 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.