FvH4_3g35600

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011426
Physical Location & Seq
Reverse (-)
30574236 .. 30578123
3888 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35600.t1

Sequence Viewer

Length: 1425 bp
ATGGGAGACTCGTTGTATGGGATCCCTAAAGAGGCCAATCTTGTTATGTCTGGGAGAAAGCAGTCTCCAAGTTACTTAGTTGCTTCGCGCTCCAAGGCCTCTTTGCAGAGCTTGGGCGCGTTTAGAATTGGAAATGGTGTGGTCAGCCAGGCAGCACTGTTTGTGGTCAAAGTGGCTGCATTGGAGGCGGTACGGAGGCTCTCCAGGGCCAAGTGCCCTTGGGTGTGGCGCGGCTTCCAGGCTTTGCAGTTTCTGTGTTATCCACCGTTTAAGTGGTTTCGGAGATTCGCTCCCTTCAAGGCTCTCGTTGACAACTTGCAGACGGTATCGAAGCCGTTACTGGCTCTCTCCATTGGAACGGCTTTTACTGATGAGTTAGAGTTTGGTCCTGGAACTAAGGATGATGTCAACAATTCTGATGCGCGTCCAGAAGTACCGTCTGCAGAGTCTTCTTTGGATACAAGTGTTGCTGAAGTAGTTCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTACATAATGAGCTCGAAAGACAAGGGCTTAGCTTGCCAGAAAGAATTGACGAGGAGGAACTTCGCAGGTTCTACACAGCGGCTAACAGAGACTTCTCGAGCTTTATGTCTGCAATAAAGAAGACGATCAGGTGGCGAGAGACTTATGGAATTCTTTCGGTACAGGAGCTTGAGAAATGGTCAAATATGGTCTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTCATAGTACGGCTTGGAATAGCATGCATCAGCTTGCCAGCTCATGATAAACCTCGGTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATACTCAGATTACAGTTGTAGTAGATTGTGAAGGTCTGACTCCATTGAAAATTCCCATGCAAGTATTGAGAACCTGTTCTTCGCTATTGCAAGATCACTTCCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCTATGCTTCGTGTTATTGCCCAAACTTTTATCCAAGTTTTAAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAAAATGTACCAAAGGATTCTTTCTGAGTGCCTGGAGACACTCCCATCATTTCTTGGTGGCAAATGCCTGTGCAAAATATGTTCAGATATTAACACGAGAGACATGCAGCAGCCTTGCATAAATGATATTATGAAGAGAGAGCTGAGAACGAGTGTTTGCGAGGATGGGAATTTTCGCTCTCCCAGTTCAATTTGTGAGTCTGAGGTTGAACCAGACTATAACTGTGATCGGCTTCTGAGAACTGCCATAGTCAGCATCCTTATGTTTTGGATTTTAGTAGCTTTTATTGCTGGATTTTTTGACCCTGGAAGCGGTCCCTTTTCTTCTTCAAGAGGTAAGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

475

Amino Acids

53.45

Weight (kDa)

8.75

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 233 - 380 1.4e-23 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 573
AccII CGCG 4 cut(s) 88, 119, 231, 424
AciI CCGC 4 cut(s) 188, 231, 596, 1392
AclWI GGATC 2 cut(s) 16, 29
AcsI RAATTY 3 cut(s) 666, 912, 1249
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 6 cut(s) 192, 435, 678, 753, 993, 1088
AfiI CCNNNNNNNGG 2 cut(s) 31, 1391
AgsI TTSAA 6 cut(s) 298, 910, 1076, 1269, 1289, 1410
AjnI CCWGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
AluBI AGCT 9 cut(s) 111, 529, 549, 618, 685, 777, 785, 1222, 1361
AluI AGCT 9 cut(s) 111, 529, 549, 618, 685, 777, 785, 1222, 1361
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 5 cut(s) 69, 600, 650, 1109, 1173
AlwI GGATC 2 cut(s) 16, 29
AlwNI CAGNNNCTG 1 cut(s) 253
Ama87I CYCGRG 2 cut(s) 613, 1056
AoxI GGCC 3 cut(s) 33, 96, 207
ApeKI GCWGC 4 cut(s) 152, 176, 1186, 1189
ApoI RAATTY 3 cut(s) 666, 912, 1249
Asp700I GAANNNNTTC 3 cut(s) 477, 670, 937
AspLEI GCGC 4 cut(s) 90, 119, 231, 424
AspS9I GGNCC 3 cut(s) 207, 386, 1394
AvaI CYCGRG 2 cut(s) 613, 1056
AvaII GGWCC 2 cut(s) 386, 1394
BaeGI GKGCMC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 450, 483
BamHI GGATCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 531
BauI CACGAG 1 cut(s) 1174
BbsI GAAGAC 3 cut(s) 441, 644, 700
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 4 cut(s) 163, 164, 1198, 1201
BccI CCATC 3 cut(s) 498, 1132, 1238
BceAI ACGGC 4 cut(s) 319, 375, 770, 1010
BcgI CGANNNNNNTGC 2 cut(s) 1165, 1199
BciT130I CCWGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
BciVI GTATCC 1 cut(s) 451
BclI TGATCA 1 cut(s) 829
BcoDI GTCTC 5 cut(s) 69, 600, 650, 1109, 1173
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 573
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 6 cut(s) 153, 177, 232, 597, 1187, 1190
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 6 cut(s) 154, 178, 233, 598, 1188, 1191
Bme1390I CCNGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
Bme18I GGWCC 2 cut(s) 386, 1394
BmeT110I CYCGRG 2 cut(s) 613, 1056
BmgT120I GGNCC 3 cut(s) 207, 386, 1394
BmiI GGNNCC 2 cut(s) 23, 1396
BmrFI CCNGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 5 cut(s) 409, 503, 780, 844, 1344
BmuI ACTGGG 1 cut(s) 1257
BpiI GAAGAC 3 cut(s) 441, 644, 700
BplI GAGNNNNNCTC 2 cut(s) 274, 306
BpmI CTGGAG 2 cut(s) 187, 1133
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 3 cut(s) 707, 792, 807
BsaJI CCNNGG 5 cut(s) 93, 204, 218, 797, 1384
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 1391
Bse1I ACTGG 2 cut(s) 345, 1263
BseBI CCWGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
BseDI CCNNGG 5 cut(s) 93, 204, 218, 797, 1384
BseGI GGATG 3 cut(s) 406, 1249, 1335
BseLI CCNNNNNNNGG 2 cut(s) 31, 1391
BseMII CTCAG 5 cut(s) 881, 1095, 1214, 1272, 1307
BseNI ACTGG 2 cut(s) 345, 1263
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 4 cut(s) 163, 164, 1198, 1201
Bsh1236I CGCG 4 cut(s) 88, 119, 231, 424
BshFI GGCC 3 cut(s) 35, 98, 209
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 2 cut(s) 613, 1056
BslFI GGGAC 1 cut(s) 1380
BslI CCNNNNNNNGG 2 cut(s) 31, 1391
BsmAI GTCTC 5 cut(s) 69, 600, 650, 1109, 1173
BsmFI GGGAC 1 cut(s) 1380
BsnI GGCC 3 cut(s) 35, 98, 209
BsoBI CYCGRG 2 cut(s) 613, 1056
Bsp1286I GDGCHC 2 cut(s) 218, 531
Bsp143I GATC 5 cut(s) 21, 642, 829, 955, 1306
Bsp1720I GCTNAGC 1 cut(s) 545
BspACI CCGC 4 cut(s) 188, 231, 596, 1392
BspANI GGCC 3 cut(s) 35, 98, 209
BspCNI CTCAG 5 cut(s) 880, 1096, 1215, 1273, 1308
BspFNI CGCG 4 cut(s) 88, 119, 231, 424
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 2 cut(s) 23, 1396
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 573
BspPI GGATC 2 cut(s) 16, 29
BsrI ACTGG 2 cut(s) 345, 1263
BssECI CCNNGG 5 cut(s) 93, 204, 218, 797, 1384
BssMI GATC 5 cut(s) 21, 642, 829, 955, 1306
BssSI CACGAG 1 cut(s) 1174
BssT1I CCWWGG 2 cut(s) 93, 218
Bst2BI CACGAG 1 cut(s) 1174
Bst2UI CCWGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
Bst4CI ACNGT 7 cut(s) 159, 267, 325, 438, 877, 971, 1304
Bst6I CTCTTC 1 cut(s) 1208
BstC8I GCNNGC 4 cut(s) 551, 769, 779, 783
BstDEI CTNAG 8 cut(s) 76, 396, 545, 867, 1104, 1223, 1281, 1316
BstF5I GGATG 3 cut(s) 406, 1249, 1335
BstFNI CGCG 4 cut(s) 88, 119, 231, 424
BstHHI GCGC 4 cut(s) 90, 119, 231, 424
BstKTI GATC 5 cut(s) 24, 645, 832, 958, 1309
BstMAI GTCTC 5 cut(s) 69, 600, 650, 1109, 1173
BstMBI GATC 5 cut(s) 21, 642, 829, 955, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 185, 550, 1367
BstNI CCWGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
BstNSI RCATGY 2 cut(s) 771, 1186
BstSCI CCNGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 4 cut(s) 88, 119, 231, 424
BstV1I GCAGC 4 cut(s) 163, 164, 1198, 1201
BstV2I GAAGAC 3 cut(s) 441, 644, 700
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 3 cut(s) 35, 98, 209
BtsCI GGATG 3 cut(s) 406, 1249, 1335
BtsIMutI CAGTG 1 cut(s) 155
BveI ACCTGC 1 cut(s) 573
Cac8I GCNNGC 4 cut(s) 551, 769, 779, 783
CaiI CAGNNNCTG 1 cut(s) 253
CciI TCATGA 1 cut(s) 787
CfoI GCGC 4 cut(s) 90, 119, 231, 424
Cfr13I GGNCC 3 cut(s) 207, 386, 1394
CseI GACGC 1 cut(s) 413
Csp6I GTAC 6 cut(s) 191, 434, 677, 752, 992, 1087
CviAII CATG 6 cut(s) 716, 768, 788, 833, 919, 1183
CviQI GTAC 6 cut(s) 191, 434, 677, 752, 992, 1087
DdeI CTNAG 8 cut(s) 76, 396, 545, 867, 1104, 1223, 1281, 1316
DpnI GATC 5 cut(s) 23, 644, 831, 957, 1308
DpnII GATC 5 cut(s) 21, 642, 829, 955, 1306
DraI TTTAAA 1 cut(s) 1044
Eam1104I CTCTTC 1 cut(s) 1208
EarI CTCTTC 1 cut(s) 1208
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 2 cut(s) 93, 218
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 386, 1394
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 492
Eco88I CYCGRG 2 cut(s) 613, 1056
EcoICRI GAGCTC 1 cut(s) 529
EcoRI GAATTC 1 cut(s) 666
EcoRII CCWGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
EcoT14I CCWWGG 2 cut(s) 93, 218
EcoT22I ATGCAT 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 2 cut(s) 93, 218
FaeI CATG 6 cut(s) 719, 771, 791, 836, 922, 1186
FaqI GGGAC 1 cut(s) 1380
FatI CATG 6 cut(s) 715, 767, 787, 832, 918, 1182
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 6 cut(s) 153, 177, 232, 597, 1187, 1190
FokI GGATG 3 cut(s) 413, 1256, 1322
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 6 cut(s) 153, 177, 232, 597, 1187, 1190
GlaI GCGC 4 cut(s) 89, 118, 230, 423
GluI GCNGC 6 cut(s) 153, 177, 232, 597, 1187, 1190
GsuI CTGGAG 2 cut(s) 187, 1133
HaeIII GGCC 3 cut(s) 35, 98, 209
HgaI GACGC 1 cut(s) 413
HhaI GCGC 4 cut(s) 90, 119, 231, 424
Hin1II CATG 6 cut(s) 719, 771, 791, 836, 922, 1186
Hin6I GCGC 4 cut(s) 88, 117, 229, 422
HinP1I GCGC 4 cut(s) 88, 117, 229, 422
HincII GTYRAC 2 cut(s) 310, 409
HindII GTYRAC 2 cut(s) 310, 409
HinfI GANTC 7 cut(s) 8, 285, 446, 837, 901, 1096, 1277
Hpy166II GTNNAC 2 cut(s) 310, 409
Hpy188I TCNGA 9 cut(s) 282, 418, 499, 870, 900, 1105, 1165, 1282, 1317
Hpy188III TCNNGA 4 cut(s) 428, 613, 788, 1410
Hpy8I GTNNAC 2 cut(s) 310, 409
HpyAV CCTTC 3 cut(s) 304, 887, 1070
HpyCH4III ACNGT 7 cut(s) 159, 267, 325, 438, 877, 971, 1304
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 550, 1367
HpyF3I CTNAG 8 cut(s) 76, 396, 545, 867, 1104, 1223, 1281, 1316
Hsp92II CATG 6 cut(s) 719, 771, 791, 836, 922, 1186
HspAI GCGC 4 cut(s) 88, 117, 229, 422
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 5 cut(s) 21, 642, 829, 955, 1306
LmnI GCTCC 3 cut(s) 95, 295, 682
Lsp1109I GCAGC 4 cut(s) 163, 164, 1198, 1201
LweI GCATC 5 cut(s) 409, 503, 780, 844, 1344
MaeIII GTNAC 2 cut(s) 71, 336
MalI GATC 5 cut(s) 23, 644, 831, 957, 1308
MboI GATC 5 cut(s) 21, 642, 829, 955, 1306
MboII GAAGA 7 cut(s) 441, 649, 700, 933, 1225, 1395, 1398
MflI RGATCY 1 cut(s) 21
MhlI GDGCHC 2 cut(s) 218, 531
MluCI AATT 8 cut(s) 126, 412, 561, 666, 912, 1071, 1249, 1269
MlyI GAGTC 5 cut(s) 2, 455, 846, 895, 1286
Mph1103I ATGCAT 1 cut(s) 773
MroXI GAANNNNTTC 3 cut(s) 477, 670, 937
MseI TTAA 3 cut(s) 270, 1043, 1170
MslI CAYNNNNRTG 1 cut(s) 1340
MspA1I CMGCKG 1 cut(s) 596
MspR9I CCNGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
MvaI CCWGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
MvnI CGCG 4 cut(s) 88, 119, 231, 424
MwoI GCNNNNNNNGC 3 cut(s) 185, 550, 1367
NdeII GATC 5 cut(s) 21, 642, 829, 955, 1306
NlaIII CATG 6 cut(s) 719, 771, 791, 836, 922, 1186
NlaIV GGNNCC 2 cut(s) 23, 1396
NsiI ATGCAT 1 cut(s) 773
NspI RCATGY 2 cut(s) 771, 1186
PaeI GCATGC 1 cut(s) 771
PaeR7I CTCGAG 1 cut(s) 613
PagI TCATGA 1 cut(s) 787
PceI AGGCCT 1 cut(s) 98
PdmI GAANNNNTTC 3 cut(s) 477, 670, 937
PfeI GAWTC 2 cut(s) 285, 1096
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 6 cut(s) 154, 178, 233, 598, 1188, 1191
PleI GAGTC 5 cut(s) 2, 454, 845, 895, 1285
PpsI GAGTC 5 cut(s) 2, 454, 845, 895, 1285
Psp124BI GAGCTC 1 cut(s) 531
Psp6I CCWGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
PspGI CCWGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
PspN4I GGNNCC 2 cut(s) 23, 1396
PspPI GGNCC 3 cut(s) 207, 386, 1394
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 253
PsuI RGATCY 1 cut(s) 21
RsaI GTAC 6 cut(s) 192, 435, 678, 753, 993, 1088
RsaNI GTAC 6 cut(s) 191, 434, 677, 752, 992, 1087
RseI CAYNNNNRTG 1 cut(s) 1340
SacI GAGCTC 1 cut(s) 531
SaqAI TTAA 3 cut(s) 270, 1043, 1170
SatI GCNGC 6 cut(s) 153, 177, 232, 597, 1187, 1190
Sau3AI GATC 5 cut(s) 21, 642, 829, 955, 1306
Sau96I GGNCC 3 cut(s) 207, 386, 1394
SchI GAGTC 5 cut(s) 2, 455, 846, 895, 1286
ScrFI CCNGG 6 cut(s) 149, 205, 239, 390, 1112, 1386
SduI GDGCHC 2 cut(s) 218, 531
SfaNI GCATC 5 cut(s) 409, 503, 780, 844, 1344
SfcI CTRYAG 1 cut(s) 441
Sfr274I CTCGAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 386, 1394
SlaI CTCGAG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 1340
SmlI CTYRAG 4 cut(s) 613, 686, 807, 822
SmoI CTYRAG 4 cut(s) 613, 686, 807, 822
SphI GCATGC 1 cut(s) 771
Sse9I AATT 8 cut(s) 126, 412, 561, 666, 912, 1071, 1249, 1269
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 4 cut(s) 188, 231, 596, 1392
SstI GAGCTC 1 cut(s) 531
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 6 cut(s) 147, 203, 237, 388, 1110, 1384
StyI CCWWGG 2 cut(s) 93, 218
TaaI ACNGT 7 cut(s) 159, 267, 325, 438, 877, 971, 1304
TaqI TCGA 3 cut(s) 329, 531, 614
TasI AATT 8 cut(s) 126, 412, 561, 666, 912, 1071, 1249, 1269
TauI GCSGC 2 cut(s) 234, 599
TfiI GAWTC 2 cut(s) 285, 1096
Tru1I TTAA 3 cut(s) 270, 1043, 1170
Tru9I TTAA 3 cut(s) 270, 1043, 1170
TscAI CASTG 1 cut(s) 162
TseI GCWGC 4 cut(s) 152, 176, 1186, 1189
TspDTI ATGAA 2 cut(s) 833, 1226
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 386, 1394
XapI RAATTY 3 cut(s) 666, 912, 1249
XceI RCATGY 2 cut(s) 771, 1186
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XhoI CTCGAG 1 cut(s) 613
XmnI GAANNNNTTC 3 cut(s) 477, 670, 937
Zsp2I ATGCAT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.