Rh5CG457200

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011426
Physical Location & Seq
Reverse (-)
63511305 .. 63514323
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG457200.1

Sequence Viewer

Length: 1218 bp
ATGATATTCTTGCATTTTGGGTATTTGGATGAGATTGTTCTATTTCCCAAAATTTTTATTTACCTGTATCATTATGTGGGACATTTGATTGCCAAAATAGTTGCATTCTTCCAGACGGTATCGAAGCCATTACTAGCCCTCTCCATTGCAACGGCTTTTACTGATGAATTAGAATTCGGTCCTAGAACTTCAGATGATGTCAACAATTCTGAGGCGCGTCCAGAAGTACACCCAGAAGTATCCCCTGCAGAGTCTGCTTTGGATACAAGTGTTTCTGAAGAAGTGCCTCAAAATGTAGCATCTGAAAGATGGTTGATACAACTTCATAACGAGCTCGAAAGACAAGGGCTTAGCTTGCCAGAAAGAATTGATGACGAGGAACTTCGCAGGTTCTACACAGCTGCTAACAGAGACTTCTCAAGCTTTCTGTCTGCAATAAAGAAGACAATCAGGTGGAGAAAGACTTATGGAATTCTTTCGGTACAGGAGCTTGAGAAATGGTCAAATATGGTTTTCTGGCATGGATTTGATGTGAAACACCGACCTTGCCTTATAGTACGGCTTGGACTAGCTTGCATCAGCTTGCCATCTCACGATAAACCTCGCTTTGCTCAAGCAATCATTTCTCAAGTTGATCATGGAGTCTTTCATTTACTTGATGCCAACAATTCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTAACTCCATTGAAAATTCCCATGCAAGTATTGAGAACCTGTTCTTCGCTATTGCAAGATCACTTTCCCAACCGTCTTGGCTGTCTGTTTGTTGTACGGCTTCCTCCAATGCTTCGTGTTATTGCCCAAACTTTCATCCAAGTTTTGAAACCTTACACCCGAGAAAAGTTGAGAATTGAAGGGAAGATGTACCGGAAGAGTCTCTCTGAGTGCCTAGAGACACTCCCTTCATGTCTTGGTGGCAAATGCATGTGCAGAATATGTTCAGAAATCAACACAAAAGATATGCAGCAGCCTCACACAAATGATATTATGAAGAGAGAGCTGAAAGCGAGTGTTTGCGAGGATGGGAGTTTATGCTCTCCCGGTCCAACTTGTGAGACCGAGGTTGAACCGAACCATTACTGTGATCAGCTGCTGAGAACTGCCATAGTGAGCATCCTTATGTTTTGGATTTTAGTAGCTTTTATTGCTGGATTATATGACCCTGGAAGCAGTCCCTTTTCTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

405

Amino Acids

46.01

Weight (kDa)

6.13

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 168 - 315 3.7e-24 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccII CGCG 1 cut(s) 217
AcsI RAATTY 4 cut(s) 51, 173, 471, 717
AcuI CTGAAG 2 cut(s) 174, 297
AfaI GTAC 5 cut(s) 228, 483, 558, 798, 893
AgsI TTSAA 4 cut(s) 715, 850, 881, 1094
AjnI CCWGG 1 cut(s) 1189
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 4 cut(s) 405, 908, 914, 1076
AlwNI CAGNNNCTG 2 cut(s) 254, 1120
Ama87I CYCGRG 1 cut(s) 861
ApeKI GCWGC 4 cut(s) 401, 991, 994, 1117
ApoI RAATTY 4 cut(s) 51, 173, 471, 717
Asp700I GAANNNNTTC 3 cut(s) 475, 742, 964
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 179, 1070
AsuC2I CCSGG 1 cut(s) 1068
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 2 cut(s) 179, 1070
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 4 cut(s) 388, 1003, 1006, 1104
BccI CCATC 3 cut(s) 303, 595, 1043
BceAI ACGGC 3 cut(s) 168, 575, 815
BciT130I CCWGG 1 cut(s) 1191
BciVI GTATCC 2 cut(s) 250, 256
BclI TGATCA 2 cut(s) 634, 1111
BcnI CCSGG 1 cut(s) 1068
BcoDI GTCTC 4 cut(s) 405, 908, 914, 1076
BfaI CTAG 4 cut(s) 134, 183, 569, 917
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 2 cut(s) 250, 256
BisI GCNGC 4 cut(s) 402, 992, 995, 1118
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 4 cut(s) 403, 993, 996, 1119
Bme1390I CCNGG 2 cut(s) 1068, 1191
Bme18I GGWCC 2 cut(s) 179, 1070
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 2 cut(s) 179, 1070
BmrFI CCNGG 2 cut(s) 1068, 1191
BmsI GCATC 4 cut(s) 308, 585, 649, 1149
BpiI GAAGAC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 350
BpuEI CTTGAG 4 cut(s) 403, 512, 597, 612
BpuMI CCSGG 1 cut(s) 1068
BsaI GGTCTC 1 cut(s) 1076
BsaJI CCNNGG 2 cut(s) 1086, 1189
BsaWI WCCGGW 1 cut(s) 894
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 1191
BseDI CCNNGG 2 cut(s) 1086, 1189
BseGI GGATG 4 cut(s) 34, 837, 1054, 1140
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 3 cut(s) 201, 900, 1112
BseXI GCAGC 4 cut(s) 388, 1003, 1006, 1104
BsgI GTGCAG 1 cut(s) 976
Bsh1236I CGCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 2 cut(s) 895, 1068
BslFI GGGAC 2 cut(s) 93, 1185
BsmAI GTCTC 4 cut(s) 405, 908, 914, 1076
BsmFI GGGAC 2 cut(s) 93, 1185
BsmI GAATGC 1 cut(s) 104
Bso31I GGTCTC 1 cut(s) 1076
BsoBI CYCGRG 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 3 cut(s) 634, 760, 1111
Bsp1720I GCTNAGC 1 cut(s) 350
BspCNI CTCAG 3 cut(s) 202, 901, 1113
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 1214
BspMAI CTGCAG 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 1076
BsrDI GCAATG 1 cut(s) 144
BssECI CCNNGG 2 cut(s) 1086, 1189
BssMI GATC 3 cut(s) 634, 760, 1111
Bst2UI CCWGG 1 cut(s) 1191
Bst4CI ACNGT 4 cut(s) 118, 682, 776, 1109
Bst6I CTCTTC 2 cut(s) 893, 1013
BstAPI GCANNNNNTGC 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 356, 574, 584
BstDEI CTNAG 4 cut(s) 210, 350, 909, 1121
BstF5I GGATG 4 cut(s) 34, 837, 1054, 1140
BstFNI CGCG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 3 cut(s) 637, 763, 1114
BstMAI GTCTC 4 cut(s) 405, 908, 914, 1076
BstMBI GATC 3 cut(s) 634, 760, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 254, 355, 1172
BstNI CCWGG 1 cut(s) 1191
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 2 cut(s) 1066, 1189
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 4 cut(s) 388, 1003, 1006, 1104
BstV2I GAAGAC 1 cut(s) 449
BsuI GTATCC 2 cut(s) 250, 256
BtsCI GGATG 4 cut(s) 34, 837, 1054, 1140
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 356, 574, 584
CaiI CAGNNNCTG 2 cut(s) 254, 1120
CciI TCATGA 1 cut(s) 1214
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 179, 1070
CseI GACGC 1 cut(s) 206
Csp6I GTAC 5 cut(s) 227, 482, 557, 797, 892
CviAII CATG 6 cut(s) 521, 638, 724, 933, 952, 1215
CviQI GTAC 5 cut(s) 227, 482, 557, 797, 892
DdeI CTNAG 4 cut(s) 210, 350, 909, 1121
DpnI GATC 3 cut(s) 636, 762, 1113
DpnII GATC 3 cut(s) 634, 760, 1111
Eam1104I CTCTTC 2 cut(s) 893, 1013
EarI CTCTTC 2 cut(s) 893, 1013
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 1076
Eco47I GGWCC 2 cut(s) 179, 1070
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 174, 297
Eco88I CYCGRG 1 cut(s) 861
EcoICRI GAGCTC 1 cut(s) 334
EcoRI GAATTC 2 cut(s) 173, 471
EcoRII CCWGG 1 cut(s) 1189
EcoT22I ATGCAT 1 cut(s) 953
EcoT38I GRGCYC 1 cut(s) 336
FaeI CATG 6 cut(s) 524, 641, 727, 936, 955, 1218
FaqI GGGAC 2 cut(s) 93, 1185
FatI CATG 6 cut(s) 520, 637, 723, 932, 951, 1214
FbaI TGATCA 2 cut(s) 634, 1111
Fnu4HI GCNGC 4 cut(s) 402, 992, 995, 1118
FokI GGATG 4 cut(s) 41, 824, 1061, 1127
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 4 cut(s) 402, 992, 995, 1118
FspBI CTAG 4 cut(s) 134, 183, 569, 917
GlaI GCGC 1 cut(s) 216
GluI GCNGC 4 cut(s) 402, 992, 995, 1118
HapII CCGG 2 cut(s) 895, 1068
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 217
Hin1II CATG 6 cut(s) 524, 641, 727, 936, 955, 1218
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 421
HinfI GANTC 3 cut(s) 251, 642, 901
HpaII CCGG 2 cut(s) 895, 1068
Hpy166II GTNNAC 2 cut(s) 202, 229
Hpy188I TCNGA 6 cut(s) 193, 211, 277, 304, 910, 970
Hpy188III TCNNGA 4 cut(s) 112, 221, 593, 1215
Hpy8I GTNNAC 2 cut(s) 202, 229
HpyAV CCTTC 3 cut(s) 692, 875, 939
HpyCH4III ACNGT 4 cut(s) 118, 682, 776, 1109
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 355, 1172
HpyF3I CTNAG 4 cut(s) 210, 350, 909, 1121
Hsp92II CATG 6 cut(s) 524, 641, 727, 936, 955, 1218
HspAI GCGC 1 cut(s) 215
Ksp22I TGATCA 2 cut(s) 634, 1111
Kzo9I GATC 3 cut(s) 634, 760, 1111
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 4 cut(s) 388, 1003, 1006, 1104
LweI GCATC 4 cut(s) 308, 585, 649, 1149
MaeI CTAG 4 cut(s) 134, 183, 569, 917
MalI GATC 3 cut(s) 636, 762, 1113
MboI GATC 3 cut(s) 634, 760, 1111
MboII GAAGA 9 cut(s) 100, 290, 454, 738, 898, 910, 1030, 1200, 1203
MhlI GDGCHC 1 cut(s) 336
MlyI GAGTC 3 cut(s) 260, 651, 910
MmeI TCCRAC 1 cut(s) 1097
MnlI CCTC 9 cut(s) 149, 205, 297, 370, 612, 816, 1008, 1039, 1081
Mph1103I ATGCAT 1 cut(s) 953
MroXI GAANNNNTTC 3 cut(s) 475, 742, 964
MslI CAYNNNNRTG 3 cut(s) 1005, 1107, 1145
MspA1I CMGCKG 2 cut(s) 401, 1117
MspI CCGG 2 cut(s) 895, 1068
MspR9I CCNGG 2 cut(s) 1068, 1191
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 1191
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 254, 355, 1172
NciI CCSGG 1 cut(s) 1068
NdeII GATC 3 cut(s) 634, 760, 1111
NlaIII CATG 6 cut(s) 524, 641, 727, 936, 955, 1218
NsiI ATGCAT 1 cut(s) 953
NspI RCATGY 1 cut(s) 955
PagI TCATGA 1 cut(s) 1214
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 3 cut(s) 475, 742, 964
PkrI GCNGC 4 cut(s) 403, 993, 996, 1119
PleI GAGTC 3 cut(s) 259, 650, 909
PpsI GAGTC 3 cut(s) 259, 650, 909
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 1189
PspGI CCWGG 1 cut(s) 1189
PspPI GGNCC 2 cut(s) 179, 1070
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 2 cut(s) 254, 1120
PvuII CAGCTG 2 cut(s) 401, 1117
RsaI GTAC 5 cut(s) 228, 483, 558, 798, 893
RsaNI GTAC 5 cut(s) 227, 482, 557, 797, 892
RseI CAYNNNNRTG 3 cut(s) 1005, 1107, 1145
SacI GAGCTC 1 cut(s) 336
SatI GCNGC 4 cut(s) 402, 992, 995, 1118
Sau3AI GATC 3 cut(s) 634, 760, 1111
Sau96I GGNCC 2 cut(s) 179, 1070
SchI GAGTC 3 cut(s) 260, 651, 910
ScrFI CCNGG 2 cut(s) 1068, 1191
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 4 cut(s) 308, 585, 649, 1149
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 2 cut(s) 179, 1070
SmiMI CAYNNNNRTG 3 cut(s) 1005, 1107, 1145
SmlI CTYRAG 4 cut(s) 418, 491, 612, 627
SmoI CTYRAG 4 cut(s) 418, 491, 612, 627
SspMI CTAG 4 cut(s) 134, 183, 569, 917
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 1066, 1189
TaaI ACNGT 4 cut(s) 118, 682, 776, 1109
TaqI TCGA 2 cut(s) 122, 336
TaqII GACCGA 2 cut(s) 167, 1100
TatI WGTACW 1 cut(s) 226
TseI GCWGC 4 cut(s) 401, 991, 994, 1117
TspDTI ATGAA 7 cut(s) 180, 314, 638, 826, 921, 1031, 1203
VpaK11BI GGWCC 2 cut(s) 179, 1070
XapI RAATTY 4 cut(s) 51, 173, 471, 717
XceI RCATGY 1 cut(s) 955
XmnI GAANNNNTTC 3 cut(s) 475, 742, 964
XspI CTAG 4 cut(s) 134, 183, 569, 917
Zsp2I ATGCAT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.