AT3G50590

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008489
Physical Location & Seq
Forward (+)
18770872 .. 18779502
8631 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50590.1

Sequence Viewer

Length: 4845 bp
ATGGAGTGGGCAACGGTGCAGCATCTGGATCTGCGTCATGTAGGTCGCGGTGTGAGCAAGCCACTACAACCTCATACGGCGGCGTTTCATCCTTCTCAAGCCGTTATCGCTGTTGCCGTTGGCTCTCACATCATGGAATTTGATGCATTAACGGGATGTAAGATCGCGTCTATTGATATTGGATCTCCAGCTGTTAGAATGTTGTATAGTCCTACTAGCAGCAATGCTGTTGTTGCCATTCTTGAGGATTGTACAATTAGGTCTTGTGATTTTGAAACGGAGCAGACTTGTGTATTGCACTCACCGGAGAAGAGGAGTGAGCACATTTCCTCAGATACTGAAGTTCATCTTGCTGTGACGCCACTTCAACCTGTTGTGTTCTTTGGATTCCCAAAACGAATGAGTGTCACAGTTGTTGGAACTGTTGAAGGTGGAAGAGCTCCAACCAAAATTAAGACAGATTTGAAGAAACCAATTGTGAACATTGCTTGCCATCCTCGACTACCTGTCCTGTATGTAGCATATGCAGAGGGGTTGATACGAGCTTATAACATTCATACTTATGCTGTTCATTATACACTACAGCTTGATAATACTATTAAACTTATCGGCGCAAGTTCGTTTGCTTTTCACCCAACACTAGAATGGATTTTTGTTGGAGATAGGAGGGGTACACTTTTAGCGTGGGATGTTTCAACTGAAAGACCTAATATGATTGGAATAACGCAAGTGGGTTCGCAACCCATCACTTCAATTTCTTGGCTTCCTATGTTGCGTGTACTTGTCACTGTGTCCAAGGATGGATCTCTTCAAGTGTGGAAGACACGAGTAATAATTAATCCCAATAGGCCTTCGACACAAACAAACTTCTTTGAGCCTGCTGCTATGGAATCAATAGACATTCCTAAAATTCTCTCCCAACAGGGTGGCGAAGCTGTTTACCCTTTACCACGAATCAAAACTTTAGAAGTTCACCCAAAGCTGAACTTAGCAGCTTTGATTTTTGCAAACATGGTGGGTAATGAGAACACTCAAAATCGAGCAGCTCAGACTAGAGAAGGAAGGAAGCAGCTCTTTGCAGTTTTGCAAAGTGCTAGGGGATCTTCAGCTTCTGTTCTAAAGGAAAAGCTTTCGTCTATGGGTTCCTCTGGAATTTTAGCCGAGCATCAACTTCAAGCTCTGCTACAGGAGCACAAGGGCCAGAGTCAGCTAACAATTTCAGATATCGCACGGAAGGCGTTCTTGTATAGTCATTTTATGGAGGGTCATGCCAAAACTGCTCCAATATCAAGGTTACCCCTTATTACGGTCGTGGATACAAAGGATCAACTTAAAGACATTCCTGTTTGCCAGCCATTTCATTTGGAGCTGAATTTCTTCAATAAGCCAAATAGAGTTCTTCATTATCCTGTGAGGTCCTTTTATATCGAGGGCCTTAACCTCATGGCTCACAATCTCTGTTCTGGAACAGACAACATCTACAAGAAGCTGTACACATCGATCCCCGGAAATGTTGAATATCATTCGAAACACATTGTTTACAGTCGTAAAAGGCACCTCTTTCTGGTTGTCTTTGAGTTCAGTGGTGCTACAAATGAAGTTGTGCTTTACTGGGAAAATACAGGTTCCCAGCTGCCTAATAGCAAAGGAAGCACTGCTAAAGGTTGTGATGCTGCCTTTATCGGCCCAAATGACGATCAATTTGCAATTCTTGACGAGGATAAGACAGGACTGTCTATGTATATCCTCCCAAAACTTACCACAATGGAAGAGAATGAGAAGAATTTGTTATCTGAAGAAAACCAAAAGAAAGAGGCGGATCCTTCTGGAATTCAGGGTCCACAACAATTCATGTTTGAGACAGAAGTCGATCGTGTTTTTTCCACACCGATAGAGTCCACTCTGATGTTTGCTTGTAATGGAACCCAAATTGGGCTGGCAAAGTTGTTTCAAGGCTACCGTCTCTCAGCTTCAGATGGTCATTATATTTCAACACAAGGTGAGGGACGAAAGTCAATCAAGTTGAAGAAACATGAAATAGCTCTTCAGTCGTTTTATTCCTTACCCTTTAATGTGGTTTACATCTGGCCGGTTCTCTTTGAAATTAAAACGATCATGTTCAGAAAATTGACTCGTCATGTTCAATGGCAAGAAACACCCCGGGGATATGTCGCAGGGATATTAACAACCCAAAGGGTGCTGATGGTATCTCTTCTATGGGTTGGGCCTGCCTTACTCTTTTCTACAACAACTGCTGTATGTTTACTTGGCTGGGATGGGAAAGTGAGGACTATTCTTTCCATAAGTACACCATACGCAGCCCTAGTTGGTGCCTTGAATGACCGCCTATTGCTTGCTCATCCCACTGATATAAGTCCGAAGCAGAAAAAGGGGATCGAAATCAAGAGCTGCCTTGTTGGGCTACTTGAACCGCTTTTAATTGGATTTTCTACAATGCAACAAACATTTGAACAGAAAGTAGACCTTTCTGAAATATTGTATCAAATAACAACAAGGTTTGATAGCTTGCGAATTACTCCAAGATCTCTTGATATTCTTGCTAGAAGCGCTCCAGTATGTGGAGATCTTGCTGTATCACTAGCTCAAGCAGGCCCTCAATTCAACCAGGTATTACGTTGTGCATATGCGATAAAGGCTCTTCGGTTCTCCACGGCATTGTCAGTTTTAAAGGACGAGTTCTTGCGTTCTAGAGACTATCCAAAATGTCCTCCAACTTCTCTTTTGTTCCAGAGGTTCCGGCAGCTTGGATATGCCTGTATCAAATATGGTCAATTCGATAGCGCAAAGGAAACTTTTGAAGTAATAGGCGACTATGAGAGCATGCTAGATCTTTTTATATGCCACCTCAACCCTAGTGCTATGCGTCGTCTTGCCCAGAAATTGGAAGAAGAAAGTGGAGATCCCGAGTTGAGACGATATTGTGAAAGGATTTTACGAGTACGGTCTACAGGATGGACCCAGGGAATTTTTGCTAATTTTGCAGCTGAGAGTATGGTTCCAAAAGGACCAGAATGGGGTGGTGGCAACTGGGAAATCAAGACTCCAACCGACATGAAGAGCATACCTAAGTGGGAGCTTGCTGGGGAAGTAATGCCATACATGAAGAATGAAGATGGAACCATCCCCTCAATAGTAGCTGATCATATTGGTGTTTACTTGGGCTGTGTCAAAGGCAGAGTTAATGTGGTGGAAATTAAAGAAGACAGCTTAGTTTCTAAACCTGGAGGGCTTAGTTTGTTGGGTAAACCTGTATCGGATAAACCTCTGGCACTTCCTGCTGGTGAATCAAGTTCGATGATGGGTCTGGAATCCCTTGGAAAACAAAACGTAGCTGATGAACAGGCAAAGGCTGCTGAGGAATTCAAGAAAACAATGTATGGTGCTACTGGTGATGGCAGCAGCAGTGACGAGGAAGGTGTAACAAAACCTAAGAAGCTGCAGATTAGAATACGAGAAAAGCCAACATCTACCACCGTGGACGTCAATAAGCTTAAAGAAGCTGCTAAAACGTTTAAACTCGGAGATGGTCTAGGCTTAACAATGAGTAGAACAAAATCAATTAATGCTGGATCTCAGGATTTGGGTCAGATGTTAAGCCAGCCTTCTTCCTCAACAGTTGCAACTACAACTGCTCCAAGTTCTGCATCTGCTCCTGTTGATCCTTTTGCAATGAGCTCATGGACACAGCAACCTCAACCAGTTTCTCAACCAGCTCCACCTGGTGTAGCCGCGCCGATACCGGAAGACTTTTTCCAAAACACAATCCCATCTGTTGAGGTTGCCAAGACATTGCCTCCTCCTGGCACTTATCTTTCGAAAATGGACCAAGCTGCTCGAGCTGCAATAGCTGCACAGGGTGGTCCAAATCAAGCAAATAATACTCCTCTGCCTGATATTGGTCTTCCCGATGGTGGTGTTCCTCAACAGTATCCTCAACAAACAAGTCAACAACCTGGTGCGCCATTTCAGACTGTTGGACTTCCTGACGGCGGTGTTCGTCAACAGTATCCTGGTCAGAACCAGGTGCCATCTCAGGTTCCCGTTTCTACACAGCCTCTTGATCTTAGCGTTCTTGGAGTTCCAAATACCGGCGATTCTGGTAAGCCTCCTGGACAACCTCAATCGCCTCCTGCATCAGTTCGTCCTGGACAGGTTCCACGTGGAGCTGCAGCTCCGGTTTGTTTCAAGACTGGACTTGCCCATCTTGAACAAAACCAGCTTCCAGATGCACTGTCTTGCTTTGACGAAGCATTCTTGGCTCTGGCTAAGGATCAATCGCGTGGTGCAGATATCAAAGCTCAAGCCACAATCTGTGCTCAGTACAAGATAGCTGTAACTCTCCTTCGGGAAATCTTGAGGCTACAAAGAGTCCAAGGTGCAAGTGCATTAAGCGCAAAGGACGAAATGGCAAGACTTTCAAGGCATCTAGCTTCACTGCCACTACTAGCCAAGCACCGTATAAACTGCATACGTACTGCAATCAAAAGAAACATGGAAGTTCAAAACTATGGCTACTCAAAGCAGATGCTGGAACTTCTTTTATCAAAGGCACCAGCGAGTAAGCAAGAGGAATTAAGAGGACTAGTCGATCTCTGTGTCCAGAGAGGCACATCGAACAAATCCATTGACCCGCTTGAAGATCCTTCTCAGTTATGCTCAGCTACGCTCAGCAGATTATCGACTATTGGATACGATGTTTGTGATCTCTGTGGTGCTAAGTTTGCTGCTCTTTCGTCGCCCGGTTGTATCATATGCGGTATGGGAAGCATCAAACGCTCTGATGCACTCGCTGGACCTGCACCTGTATCCACACCGTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1614

Amino Acids

176.3

Weight (kDa)

8.6

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 153 - 275 8.6e-07 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 154 - 273 3.1e-08 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 156 - 278 4.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 162 - 276 7.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AasI GACNNNNNNGTC 1 cut(s) 1732
AatII GACGTC 1 cut(s) 3504
Acc36I ACCTGC 1 cut(s) 4825
AccB1I GGYRCC 4 cut(s) 1554, 2330, 4045, 4570
AccB7I CCANNNNNTGG 2 cut(s) 2579, 2902
AccI GTMKAC 2 cut(s) 2481, 2966
AccII CGCG 4 cut(s) 48, 167, 3752, 4300
AciI CCGC 9 cut(s) 48, 80, 1817, 2344, 2432, 3750, 4011, 4652, 4776
AclI AACGTT 1 cut(s) 3530
AcoI YGGCCR 1 cut(s) 2087
AcsI RAATTY 8 cut(s) 137, 909, 1152, 1372, 1783, 1830, 2985, 3380
AcuI CTGAAG 5 cut(s) 360, 1089, 1815, 1956, 2030
AcvI CACGTG 1 cut(s) 4181
AcyI GRCGYC 2 cut(s) 359, 3501
AdeI CACNNNGTG 3 cut(s) 2000, 3743, 3878
AfaI GTAC 8 cut(s) 253, 673, 780, 1493, 2308, 2961, 4343, 4495
AfeI AGCGCT 1 cut(s) 2569
AhdI GACNNNNNGTC 1 cut(s) 506
AhlI ACTAGT 1 cut(s) 4603
Alw21I GWGCWC 5 cut(s) 324, 442, 1194, 3698, 4339
Alw26I GTCTC 4 cut(s) 1853, 1967, 2706, 2926
AlwNI CAGNNNCTG 4 cut(s) 25, 338, 1112, 4549
Ama87I CYCGRG 3 cut(s) 2160, 2924, 3855
Aor51HI AGCGCT 1 cut(s) 2569
AoxI GGCC 7 cut(s) 848, 1198, 1432, 1684, 2087, 2225, 2611
ApoI RAATTY 8 cut(s) 137, 909, 1152, 1372, 1783, 1830, 2985, 3380
ArsI GACNNNNNNTTYG 2 cut(s) 1685, 1717
AseI ATTAAT 2 cut(s) 837, 3582
Asp700I GAANNNNTTC 2 cut(s) 1238, 1376
AspLEI GCGC 6 cut(s) 614, 2570, 2804, 3754, 3982, 4415
AsuC2I CCSGG 4 cut(s) 1506, 2161, 2162, 4761
AsuHPI GGTGA 6 cut(s) 294, 623, 965, 2012, 3314, 3422
AsuII TTCGAA 2 cut(s) 1526, 3836
AvaI CYCGRG 3 cut(s) 2160, 2924, 3855
AvaII GGWCC 7 cut(s) 1416, 1838, 2976, 3026, 3844, 3881, 4814
BaeI ACNNNNGTAYC 2 cut(s) 327, 360
BamHI GGATCC 1 cut(s) 1819
BanI GGYRCC 4 cut(s) 1554, 2330, 4045, 4570
BanII GRGCYC 2 cut(s) 442, 3698
BarI GAAGNNNNNNTAC 2 cut(s) 4347, 4379
BauI CACGAG 1 cut(s) 825
BbrPI CACGTG 1 cut(s) 4181
BbsI GAAGAC 4 cut(s) 827, 3228, 3771, 3914
Bbv12I GWGCWC 5 cut(s) 324, 442, 1194, 3698, 4339
BbvCI CCTCAGC 1 cut(s) 3375
BceAI ACGGC 5 cut(s) 86, 93, 101, 2688, 4024
BcgI CGANNNNNNTGC 2 cut(s) 1685, 1719
BciVI GTATCC 5 cut(s) 1309, 3960, 4038, 4703, 4837
BclI TGATCA 1 cut(s) 3160
BcnI CCSGG 4 cut(s) 1506, 2161, 2162, 4761
BcoDI GTCTC 4 cut(s) 1853, 1967, 2706, 2926
BcuI ACTAGT 1 cut(s) 4603
BfmI CTRYAG 5 cut(s) 581, 1184, 2967, 3458, 4188
BfoI RGCGCY 1 cut(s) 2571
BfuAI ACCTGC 1 cut(s) 4825
BfuI GTATCC 5 cut(s) 1309, 3960, 4038, 4703, 4837
BglII AGATCT 3 cut(s) 2543, 2584, 2848
BlpI GCTNAGC 2 cut(s) 4678, 4688
Bme18I GGWCC 7 cut(s) 1416, 1838, 2976, 3026, 3844, 3881, 4814
BmeRI GACNNNNNGTC 1 cut(s) 506
BmeT110I CYCGRG 3 cut(s) 2160, 2924, 3855
BmrI ACTGGG 2 cut(s) 1621, 3058
BmuI ACTGGG 2 cut(s) 1621, 3058
BoxI GACNNNNGTC 2 cut(s) 1865, 2011
BpiI GAAGAC 4 cut(s) 827, 3228, 3771, 3914
BpmI CTGGAG 3 cut(s) 171, 2556, 3264
Bpu10I CCTNAGC 2 cut(s) 3375, 4287
Bpu1102I GCTNAGC 2 cut(s) 4678, 4688
Bpu14I TTCGAA 2 cut(s) 1526, 3836
BpuEI CTTGAG 5 cut(s) 81, 263, 2589, 4305, 4396
BpuMI CCSGG 4 cut(s) 1506, 2161, 2162, 4761
Bsa29I ATCGAT 1 cut(s) 1499
BsaAI YACGTR 2 cut(s) 4181, 4493
BsaBI GATNNNNATC 1 cut(s) 2399
BsaHI GRCGYC 2 cut(s) 359, 3501
BsaWI WCCGGW 3 cut(s) 304, 3760, 4195
BsaXI ACNNNNNCTCC 4 cut(s) 3637, 3667, 3799, 3829
Bse118I RCCGGY 2 cut(s) 2089, 4109
Bse1I ACTGG 6 cut(s) 1616, 2573, 3053, 3412, 3719, 4216
Bse3DI GCAATG 4 cut(s) 229, 483, 3696, 3809
Bse8I GATNNNNATC 1 cut(s) 2399
BseCI ATCGAT 1 cut(s) 1499
BseGI GGATG 9 cut(s) 88, 161, 493, 694, 805, 2281, 2359, 2978, 3141
BseJI GATNNNNATC 1 cut(s) 2399
BseMI GCAATG 4 cut(s) 229, 483, 3696, 3809
BseNI ACTGG 6 cut(s) 1616, 2573, 3053, 3412, 3719, 4216
BseRI GAGGAG 3 cut(s) 328, 3807, 3894
BseYI CCCAGC 3 cut(s) 1629, 2271, 3101
BsgI GTGCAG 4 cut(s) 38, 3855, 4326, 4803
Bsh1236I CGCG 4 cut(s) 48, 167, 3752, 4300
Bsh1285I CGRYCG 2 cut(s) 1311, 1873
BshFI GGCC 7 cut(s) 850, 1200, 1434, 1686, 2089, 2227, 2613
BshNI GGYRCC 4 cut(s) 1554, 2330, 4045, 4570
BshVI ATCGAT 1 cut(s) 1499
BsiEI CGRYCG 2 cut(s) 1311, 1873
BsiHKAI GWGCWC 5 cut(s) 324, 442, 1194, 3698, 4339
BsiHKCI CYCGRG 3 cut(s) 2160, 2924, 3855
BsiSI CCGG 9 cut(s) 305, 1506, 2090, 2161, 2758, 3761, 4110, 4196, 4761
BslFI GGGAC 1 cut(s) 2019
BsmAI GTCTC 4 cut(s) 1853, 1967, 2706, 2926
BsmBI CGTCTC 2 cut(s) 1967, 2926
BsmFI GGGAC 1 cut(s) 2019
BsmI GAATGC 1 cut(s) 4271
BsnI GGCC 7 cut(s) 850, 1200, 1434, 1686, 2089, 2227, 2613
BsoBI CYCGRG 3 cut(s) 2160, 2924, 3855
Bsp119I TTCGAA 2 cut(s) 1526, 3836
Bsp1286I GDGCHC 5 cut(s) 324, 442, 1194, 3698, 4339
Bsp1407I TGTACA 2 cut(s) 251, 1491
Bsp1720I GCTNAGC 2 cut(s) 4678, 4688
BspACI CCGC 9 cut(s) 48, 80, 1817, 2344, 2432, 3750, 4011, 4652, 4776
BspANI GGCC 7 cut(s) 850, 1200, 1434, 1686, 2089, 2227, 2613
BspDI ATCGAT 1 cut(s) 1499
BspFNI CGCG 4 cut(s) 48, 167, 3752, 4300
BspMAI CTGCAG 2 cut(s) 3462, 4192
BspMI ACCTGC 1 cut(s) 4825
BspQI GCTCTTC 4 cut(s) 430, 2049, 2664, 3071
BspT104I TTCGAA 2 cut(s) 1526, 3836
BspT107I GGYRCC 4 cut(s) 1554, 2330, 4045, 4570
BsrDI GCAATG 4 cut(s) 229, 483, 3696, 3809
BsrFI RCCGGY 2 cut(s) 2089, 4109
BsrGI TGTACA 2 cut(s) 251, 1491
BsrI ACTGG 6 cut(s) 1616, 2573, 3053, 3412, 3719, 4216
BssAI RCCGGY 2 cut(s) 2089, 4109
BssNI GRCGYC 2 cut(s) 359, 3501
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 3 cut(s) 795, 3334, 4393
Bst2BI CACGAG 1 cut(s) 825
Bst6I CTCTTC 8 cut(s) 305, 430, 813, 1764, 2049, 2217, 2664, 3071
BstACI GRCGYC 2 cut(s) 359, 3501
BstAPI GCANNNNNTGC 3 cut(s) 3296, 3371, 3869
BstAUI TGTACA 2 cut(s) 251, 1491
BstBAI YACGTR 2 cut(s) 4181, 4493
BstBI TTCGAA 2 cut(s) 1526, 3836
BstDSI CCRYGG 2 cut(s) 2670, 3495
BstEII GGTNACC 1 cut(s) 1293
BstF5I GGATG 9 cut(s) 88, 161, 493, 694, 805, 2281, 2359, 2978, 3141
BstFNI CGCG 4 cut(s) 48, 167, 3752, 4300
BstH2I RGCGCY 1 cut(s) 2571
BstHHI GCGC 6 cut(s) 614, 2570, 2804, 3754, 3982, 4415
BstMAI GTCTC 4 cut(s) 1853, 1967, 2706, 2926
BstMCI CGRYCG 2 cut(s) 1311, 1873
BstNSI RCATGY 1 cut(s) 2845
BstPAI GACNNNNGTC 2 cut(s) 1865, 2011
BstPI GGTNACC 1 cut(s) 1293
BstSFI CTRYAG 5 cut(s) 581, 1184, 2967, 3458, 4188
BstSNI TACGTA 1 cut(s) 4493
BstUI CGCG 4 cut(s) 48, 167, 3752, 4300
BstV2I GAAGAC 4 cut(s) 827, 3228, 3771, 3914
BstXI CCANNNNNNTGG 1 cut(s) 926
Bsu15I ATCGAT 1 cut(s) 1499
BsuI GTATCC 5 cut(s) 1309, 3960, 4038, 4703, 4837
BsuRI GGCC 7 cut(s) 850, 1200, 1434, 1686, 2089, 2227, 2613
BsuTUI ATCGAT 1 cut(s) 1499
BtgI CCRYGG 2 cut(s) 2670, 3495
BtsCI GGATG 9 cut(s) 88, 161, 493, 694, 805, 2281, 2359, 2978, 3141
BtsI GCAGTG 3 cut(s) 1653, 3430, 4454
BtsIMutI CAGTG 7 cut(s) 786, 1588, 1653, 2364, 3430, 4250, 4454
BveI ACCTGC 1 cut(s) 4825
CaiI CAGNNNCTG 4 cut(s) 25, 338, 1112, 4549
CfoI GCGC 6 cut(s) 614, 2570, 2804, 3754, 3982, 4415
Cfr10I RCCGGY 2 cut(s) 2089, 4109
Cfr9I CCCGGG 1 cut(s) 2160
ClaI ATCGAT 1 cut(s) 1499
CseI GACGC 4 cut(s) 23, 156, 367, 2873
CsiI ACCWGGT 4 cut(s) 2625, 3739, 3973, 4041
Csp6I GTAC 8 cut(s) 252, 672, 779, 1492, 2307, 2960, 4342, 4494
CspCI CAANNNNNGTGG 3 cut(s) 996, 1031, 4819
CviQI GTAC 8 cut(s) 252, 672, 779, 1492, 2307, 2960, 4342, 4494
DraI TTTAAA 2 cut(s) 2688, 3535
DraIII CACNNNGTG 3 cut(s) 2000, 3743, 3878
DrdI GACNNNNNNGTC 1 cut(s) 1732
DriI GACNNNNNGTC 1 cut(s) 506
DseDI GACNNNNNNGTC 1 cut(s) 1732
EaeI YGGCCR 1 cut(s) 2087
Eam1104I CTCTTC 8 cut(s) 305, 430, 813, 1764, 2049, 2217, 2664, 3071
Eam1105I GACNNNNNGTC 1 cut(s) 506
EarI CTCTTC 8 cut(s) 305, 430, 813, 1764, 2049, 2217, 2664, 3071
EciI GGCGGA 1 cut(s) 1832
Ecl136II GAGCTC 2 cut(s) 440, 3696
Eco105I TACGTA 1 cut(s) 4493
Eco130I CCWWGG 3 cut(s) 795, 3334, 4393
Eco147I AGGCCT 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 442, 3698
Eco32I GATATC 2 cut(s) 1225, 4312
Eco47I GGWCC 7 cut(s) 1416, 1838, 2976, 3026, 3844, 3881, 4814
Eco47III AGCGCT 1 cut(s) 2569
Eco53kI GAGCTC 2 cut(s) 440, 3696
Eco57I CTGAAG 5 cut(s) 360, 1089, 1815, 1956, 2030
Eco72I CACGTG 1 cut(s) 4181
Eco88I CYCGRG 3 cut(s) 2160, 2924, 3855
Eco91I GGTNACC 1 cut(s) 1293
EcoICRI GAGCTC 2 cut(s) 440, 3696
EcoO109I RGGNCCY 3 cut(s) 1416, 1432, 2612
EcoO65I GGTNACC 1 cut(s) 1293
EcoRI GAATTC 2 cut(s) 1830, 3380
EcoRV GATATC 2 cut(s) 1225, 4312
EcoT14I CCWWGG 3 cut(s) 795, 3334, 4393
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 442, 3698
ErhI CCWWGG 3 cut(s) 795, 3334, 4393
Esp3I CGTCTC 2 cut(s) 1967, 2926
FaqI GGGAC 1 cut(s) 2019
FauI CCCGC 1 cut(s) 4659
FauNDI CATATG 3 cut(s) 523, 2644, 4772
FbaI TGATCA 1 cut(s) 3160
FblI GTMKAC 2 cut(s) 2481, 2966
FokI GGATG 9 cut(s) 75, 168, 480, 701, 812, 2288, 2346, 2985, 3128
FriOI GRGCYC 2 cut(s) 442, 3698
GlaI GCGC 6 cut(s) 613, 2569, 2803, 3753, 3981, 4414
GsaI CCCAGC 3 cut(s) 1633, 2275, 3105
GsuI CTGGAG 3 cut(s) 171, 2556, 3264
HaeII RGCGCY 1 cut(s) 2571
HaeIII GGCC 7 cut(s) 850, 1200, 1434, 1686, 2089, 2227, 2613
HapII CCGG 9 cut(s) 305, 1506, 2090, 2161, 2758, 3761, 4110, 4196, 4761
HgaI GACGC 4 cut(s) 23, 156, 367, 2873
HhaI GCGC 6 cut(s) 614, 2570, 2804, 3754, 3982, 4415
Hin1I GRCGYC 2 cut(s) 359, 3501
Hin6I GCGC 6 cut(s) 612, 2568, 2802, 3752, 3980, 4413
HinP1I GCGC 6 cut(s) 612, 2568, 2802, 3752, 3980, 4413
HincII GTYRAC 2 cut(s) 3968, 4022
HindII GTYRAC 2 cut(s) 3968, 4022
HindIII AAGCTT 2 cut(s) 1127, 3509
HpaII CCGG 9 cut(s) 305, 1506, 2090, 2161, 2758, 3761, 4110, 4196, 4761
HphI GGTGA 6 cut(s) 294, 623, 965, 2012, 3314, 3422
Hpy99I CGWCG 2 cut(s) 2889, 4759
HpyCH4IV ACGT 6 cut(s) 2635, 3348, 3501, 3530, 4180, 4492
HpySE526I ACGT 6 cut(s) 2635, 3348, 3501, 3530, 4180, 4492
Hsp92I GRCGYC 2 cut(s) 359, 3501
HspAI GCGC 6 cut(s) 612, 2568, 2802, 3752, 3980, 4413
Ksp22I TGATCA 1 cut(s) 3160
LguI GCTCTTC 4 cut(s) 430, 2049, 2664, 3071
MabI ACCWGGT 4 cut(s) 2625, 3739, 3973, 4041
MaeII ACGT 6 cut(s) 2635, 3348, 3501, 3530, 4180, 4492
MaeIII GTNAC 7 cut(s) 355, 406, 784, 1293, 3425, 3439, 4354
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 5 cut(s) 324, 442, 1194, 3698, 4339
MlyI GAGTC 5 cut(s) 1213, 1904, 2125, 3055, 4398
MmeI TCCRAC 6 cut(s) 397, 467, 637, 2756, 3089, 3976
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 2 cut(s) 1238, 1376
MslI CAYNNNNRTG 4 cut(s) 561, 643, 1904, 3168
MspA1I CMGCKG 3 cut(s) 191, 1633, 3005
MspI CCGG 9 cut(s) 305, 1506, 2090, 2161, 2758, 3761, 4110, 4196, 4761
MssI GTTTAAAC 1 cut(s) 3535
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 4271
MvnI CGCG 4 cut(s) 48, 167, 3752, 4300
NciI CCSGG 4 cut(s) 1506, 2161, 2162, 4761
NdeI CATATG 3 cut(s) 523, 2644, 4772
NmeAIII GCCGAG 1 cut(s) 1186
NmuCI GTSAC 4 cut(s) 355, 406, 784, 3425
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 2845
NspV TTCGAA 2 cut(s) 1526, 3836
PaeI GCATGC 1 cut(s) 2845
PaeR7I CTCGAG 1 cut(s) 3855
PasI CCCWGGG 1 cut(s) 2980
PceI AGGCCT 1 cut(s) 850
PciSI GCTCTTC 4 cut(s) 430, 2049, 2664, 3071
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 4271
PdmI GAANNNNTTC 2 cut(s) 1238, 1376
PfeI GAWTC 6 cut(s) 387, 890, 954, 3305, 3329, 4115
PflMI CCANNNNNTGG 2 cut(s) 2579, 2902
PfoI TCCNGGA 2 cut(s) 4129, 4165
Ple19I CGATCG 1 cut(s) 1873
PleI GAGTC 5 cut(s) 1212, 1903, 2125, 3055, 4397
PmaCI CACGTG 1 cut(s) 4181
PmeI GTTTAAAC 1 cut(s) 3535
PmlI CACGTG 1 cut(s) 4181
PpsI GAGTC 5 cut(s) 1212, 1903, 2125, 3055, 4397
Ppu21I YACGTR 2 cut(s) 4181, 4493
PpuMI RGGWCCY 1 cut(s) 1416
PshAI GACNNNNGTC 2 cut(s) 1865, 2011
PshBI ATTAAT 2 cut(s) 837, 3582
PsiI TTATAA 1 cut(s) 549
Psp124BI GAGCTC 2 cut(s) 442, 3698
Psp1406I AACGTT 1 cut(s) 3530
Psp5II RGGWCCY 1 cut(s) 1416
PspCI CACGTG 1 cut(s) 4181
PspEI GGTNACC 1 cut(s) 1293
PspFI CCCAGC 3 cut(s) 1629, 2271, 3101
PspPPI RGGWCCY 1 cut(s) 1416
PspXI VCTCGAGB 1 cut(s) 3855
PstI CTGCAG 2 cut(s) 3462, 4192
PstNI CAGNNNCTG 4 cut(s) 25, 338, 1112, 4549
PvuI CGATCG 1 cut(s) 1873
PvuII CAGCTG 3 cut(s) 191, 1633, 3005
RsaI GTAC 8 cut(s) 253, 673, 780, 1493, 2308, 2961, 4343, 4495
RsaNI GTAC 8 cut(s) 252, 672, 779, 1492, 2307, 2960, 4342, 4494
RseI CAYNNNNRTG 4 cut(s) 561, 643, 1904, 3168
SacI GAGCTC 2 cut(s) 442, 3698
SapI GCTCTTC 4 cut(s) 430, 2049, 2664, 3071
SchI GAGTC 5 cut(s) 1213, 1904, 2125, 3055, 4398
SduI GDGCHC 5 cut(s) 324, 442, 1194, 3698, 4339
SexAI ACCWGGT 4 cut(s) 2625, 3739, 3973, 4041
SfcI CTRYAG 5 cut(s) 581, 1184, 2967, 3458, 4188
Sfr274I CTCGAG 1 cut(s) 3855
SfuI TTCGAA 2 cut(s) 1526, 3836
SinI GGWCC 7 cut(s) 1416, 1838, 2976, 3026, 3844, 3881, 4814
SlaI CTCGAG 1 cut(s) 3855
SmaI CCCGGG 1 cut(s) 2162
SmiMI CAYNNNNRTG 4 cut(s) 561, 643, 1904, 3168
SmlI CTYRAG 6 cut(s) 96, 242, 2604, 3855, 4320, 4375
SmoI CTYRAG 6 cut(s) 96, 242, 2604, 3855, 4320, 4375
SnaBI TACGTA 1 cut(s) 4493
SpeI ACTAGT 1 cut(s) 4603
SphI GCATGC 1 cut(s) 2845
SseBI AGGCCT 1 cut(s) 850
SsiI CCGC 9 cut(s) 48, 80, 1817, 2344, 2432, 3750, 4011, 4652, 4776
SspI AATATT 1 cut(s) 2496
SstI GAGCTC 2 cut(s) 442, 3698
StuI AGGCCT 1 cut(s) 850
StyI CCWWGG 3 cut(s) 795, 3334, 4393
TaiI ACGT 6 cut(s) 2638, 3351, 3504, 3533, 4183, 4495
TatI WGTACW 5 cut(s) 251, 778, 1491, 2306, 4341
TauI GCSGC 2 cut(s) 83, 3752
TfiI GAWTC 6 cut(s) 387, 890, 954, 3305, 3329, 4115
TscAI CASTG 7 cut(s) 793, 1588, 1660, 2371, 3430, 4257, 4461
TseFI GTSAC 4 cut(s) 355, 406, 784, 3425
Tsp45I GTSAC 4 cut(s) 355, 406, 784, 3425
TspGWI ACGGA 2 cut(s) 293, 1246
TspMI CCCGGG 1 cut(s) 2160
TspRI CASTG 7 cut(s) 793, 1588, 1660, 2371, 3430, 4257, 4461
Van91I CCANNNNNTGG 2 cut(s) 2579, 2902
VpaK11BI GGWCC 7 cut(s) 1416, 1838, 2976, 3026, 3844, 3881, 4814
VspI ATTAAT 2 cut(s) 837, 3582
XapI RAATTY 8 cut(s) 137, 909, 1152, 1372, 1783, 1830, 2985, 3380
XbaI TCTAGA 1 cut(s) 2708
XceI RCATGY 1 cut(s) 2845
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 3855
XmaI CCCGGG 1 cut(s) 2160
XmiI GTMKAC 2 cut(s) 2481, 2966
XmnI GAANNNNTTC 2 cut(s) 1238, 1376
ZraI GACGTC 1 cut(s) 3502
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.