RLG00000017388

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008489
Physical Location & Seq
Forward (+)
16001871 .. 16013753
11883 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017388

Sequence Viewer

Length: 4908 bp
ATGGAGTGGACAACGGTGCAGCATCTCGATCTACGACACGTCGCGCGGGGCACCAAACCCCTCCAGCCTCATGCCGCCGCCTTTCATCCGCATCAGGCCCTCATCGCCGTCGCCATCGGCAACTACATCGTCGAAATGGATGCGTTGACGGGAAGCAAGATTGCATCTATTGATATCGGTATACCTGTTATCCGCATGGCATATAGTCCCACGAGTGGCCATTCTGTTATAGCGATCCATGAGGATGGTACTATTCGCTCCTGTGATTTTGATGCTGAACAAACTTGCGTGTTGCATTCACCTGAGAAGAAGTTGGATCAAATTACTCCTGATACAGAAGTACATCTTGCTTTGACACCTCTCCAACCTGTAGTGTTTTTCGGGTTCCATAAAAGGATGAGTGTTACAGTTGTTGGGACTGTTGAAGGAGGAAGGGCACCAACAAAAATAAAGACAGATTTGAAAAAACCTATTGTGAATCTTGCATGCCACCCTCGCCATCCTGTCCTGTATGTTGCTTATGCAGATGGTTTGATTCGAGCATACAACATTCACACCTATGCAGTTCATTACACCTTACAGATCGACAACACCATAAAGCTTATCGGTGCTGGAGCATTTGGTTTTCACCCAACATTGGAGTGGATTTTTGTTGGTGATAGACGGGGTACACTTCTTGCATGGGATGTTTCTACTGAGAGACCTAGTATGATTGGAATTACACAAGTGGGTTCCCAACCAATTTCTTCAGTTTCTTGGCTACCAATGTTGCGGTTACTTGTAACTGTAACCAGGGATGGAACTCTTCAAGTGTGGAAGACACGTGTTATAATTAATCCAAATAGACCTCCAATGCAAGCAAATTTCTTTGAACCTGCTGCAATTGAATCACTTGACATCCCTCGCATACTTTCTCAACAGGGTGGAGGAGCAGCTTACCCTTTACCGCGTATCAAAACATTAGAAGTTCACTCCAAACTGAATTTGGCAGCGCTTCTGTTCATAAATATGACAGGTGGAGACAATGTGAAAAATAGGGCGGCATACACTAGGGAAGGAAGGAAACAACTGTTTGCAGTTTTGCAAGGGGCAAGAGGATCCTCAGCGTCTGTTTTGAAAGAAAAGCTTTCATCTTTGGGGTCGTCTGGAATATTAGCTGAACATCAACTTCAAGCTCAACTACAAGAACATCATATGAAAGGGCACAGTCATCTTACAATATCAGACATTGCTCGGAAGGCTTTTCTCCACAGTCATTTCATGGAAGGTCATGCCAAAAGTGCCCCAATATCTCGGCTGCCTCTAATTACTATTGTAGATTCTAAGCATCATCTGAAGGATGCTCCTGTATGTCAGCCATTTCACCTGGAGCTAAATTTCTTCAGTAAAGAGAATCGGGTTCTTCATTATCCTGTCAGAGCCTTTTGTATAGACGGGTCAAACCTTATGGCGTATAATCTGTCTTCTGGAGCAGATAGTATCTACAAAAGACTTCATACATCGGTTCCTGCAAATGTGGAATACCATCCAAAGTACCTGTTCTACAGTAAAAGACAGTGCATATTTCTTGTTGTCTATGAATTTAGCGGTGCTACAAATGAAGTTGTGCTTTATTTTGAAAATACTGATTCCCAGGCAGCAAACAGTAAATGCAGCACAGTAAAAGGTCGAGATGCAGCATTTATTGGTCCCAATGAGAATCAGTTTGCAATTCTTGATGATGATAAGACTGGACTGGCTTTACATATACTGCCTGGAAAGGCTACTCCAGAAGCTAATGAAAAAAATCTACTTGCTGACGAAAACCAATCTATGAATACTGATACTGCTGCCCCCCGAGGTCCAATGCAATTTATGTTTGAAAGTGAAGTGGATCGAATCTTTTCCACTCCAATAGAGTCAACTCTGATGTTTGCTTCTCATGGGGATCAGATTGGCTTGGCAAAGCTGGTTCAAGGATACCGCCTTTCTAATGCTGGTGGTCATTATATAGCAACAACAAATGAAGGGAGAAAGTCAATCAAATTGAAACTTGATGAAATTGTACTTCAGGTTCACTGGCAAGAAACTCTTCGGGGCTATGTTGCAGGAATATTAACTACACAAAGAGTTCTTATAGTTTCAGCAGATCTAGATATACTGGCAGGAAGTTCTGCAAAATTTGATAAAGGACTTCCTTCAATATCCTTATTCAACATCTTGGTGTTAATTCCCTTGACTTCTAATCACTTTAGATCACTTTTATGGGTTGGGCCTGCACTTCTCTTTTCTACTACCACTGCAGTTAGTGTGCTTGGTTGGGATGGGAGAGTGAGGACTATACTCTCTCTCAGTATGCCTTATGCAGTTTTGGTTGGTGCACTGAATGACCGGTTGTTGCTTGCTACCCCAACAGAAATAAATCCCAGACAAAAGAAGGGAGTTGAGATTAAGAGTTGTCTTGTTGGGCTCCTCGAACCTCTTCTTATTGGCTTTGCCACAATGCAAGAAAGATTTGAGCAGAAGCTTGACCTGCCAGAGATATTGTATCAAATAACATCAAGGTTTGACAGCCTGCGTATCACTCCAAGGTCTCTTGATATTCTGGCCAGAGGCTCTCCTGTTTGTGGAGATCTTTCTGTCTCATTATCTCAAGCAGGACCACAGTTCACACAGGTATTGCGTGGTGTCTATGCTATCAAAGCTCTCCGCTTTTCTACTGCTTTATCTGTTTTGAAAGATGAATTTCTGCGCTCTAGAGATTATCCAAGGTGTCCTCCAACCTCACATCTGTTCCACCGGTTCCGGCAGTTGGGCTATGCATGTATCAAGTTTGGTCAGTTTGATAGTGCAAAAGAGACCTTTGAAGTCATAGCAGATTATGAAAGTATGCTCGATCTGTTTATATGCCACCTTAACCCCAGTGCCATGCGGCGTCTTGCTCAAAAATTGGAAGAAGATGGCACTGATTCAGAACTGAGGAGATACTGTGAGAGGATCTTAAGAGTGCGCTCTACGGGATGGACTCAAGGCATTTTTGCTAACTTTGCTGCTGAGAGTATGGTTCCGAAGGGTCCTGAATGGGGTGGTGGGAACTGGGAAATCAAAACCCCAACTAATATGAAGGCAATACCTCAATGGGAACTGGCTGCCGAGGTGATGCCATACATGAGGACTGATGACGGTGCCATTCCATCCATTATTGCTGATCATATTGGTGTTTACTTGGGTTCCATCAGAGGACGAGGCAACATTGTAGAAGTAAGGGAAGATAGCTTGGTCAAAGCTTTCAAACCTGCAGGTGGTGATAATAAGCCAAATGGTCTTCAAGACTCTTCAGTGACATCTACATCTAATATGTCCAAAGGAGTGCCTGGTGGTGGTTCATTGATGGGTCTTGAAACACTTACTAAACAAGTAGCCAGTTCCACCACGGCAGATGAACAGGCTAAAGCTGAAGAAGAATTTAAGAAATCTATGTATGGTGCAGCTGATGGTAGCAGCAGTGATGAGGAGGGAACTTCAAAAACTAAAAAGTTACAGATTCGGATTAGAGACAAGCCAATTGCTTCTACCACAGTGGATGTTGATAAGATCAAAGAAGCCACGAAGCAATTTAAACTTGGTGAGGGGTTGGCTCGCCCCAGCAGAACCAAGTCATTGACTGGATCCCAGGACCTAAGCCAGATGTTATCCCAACCGCCTCCAGCCAATAGTGGTTTTCCAAATGCTCGTGTAGGTTCCGCCCCTGGTGATCTTTTTGGTATGGATGCACCAACACAACCTGCAACTGTATCACAGCAAGCTCCTGTACCAGGTGTGGGAATGACAGCTGGACCAATTCCAGAGGACTTTTTCCAAAATACCATACCGTCCTTCCAGGTTGCAGCCTCACTGCCCCCTCCTGGAACCTATCTGTCAAAGATGGATCAAGCCTCTCAGGGGGCTGAAAGAAATACGGAAACACTTAACCAGGTCAATGCACCCAAAGCCAATATAGATCTTCCTGATGGTGGTGTTCCCCCTCAAGCTACTCAACAGGGTGTTCCACTGGGGTCCTTTGGACTTCCTGATGGCGGGGTTCCACCACAAGCCCCAAGCCAGGCTGCTGTTCAACAGACTCAGATTCAGTCAGCTCAACCTCCAATATCCTCTCAGCCTCTTGATCTAAGTGCCCTTGGCATTCCGAATTCTGCCGATAATGGAAAACCTACAGGGCAGCCTCCTTCACCACCGTCTGCTGTGCGTCCTGGACAGGTTCCCCGTGGAGCAGCTGCTCCTATATGCTTTAAGACTGGAGTATCCCACCTTGAGCAGAATCAACTTTCGGACGCATTATCCTGCTTTGATGAAGCTTTTCTTGCACTAGCGAAGGACAACTCTCGCGGAGCTGATATCAAAGCACAAGCGACCATCTGTGCTCAATATAAGATAGCAGTCACTCTTCTTCAGGAAATTGGCCGGCTTCAGAGAGTCCATGGCCCTAGTGCAATTAGTGCAAAAGATGAGATGGCAAGACTAGCACGCCACCTGGGTTCTTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATTCGAACTGCCATTAAGAGGAATATGGAAGTGCAAAATTATGCTTATTCAAAACAGATGCTTGAACTCCTGCTGTCCAAAGCACCTCCAAGTAAGCAGGATGAGCTAAGGAGCTTGGTTGACATGTGTGTTCAAAGGGGTCTGTCCAACAAGTCCATTGATCCTCTTGAAGACCCTTCCCAGTTCTGTGCGGCGACACTTAGCCGTCTGTCAACCATTGGATACGATGTTTGTGACCTTTGTGGGGCTAAATTTTCGGCTCTCGCAACGCCTGGTTGCATTATATGCGGTATGGGAAGCATCAAAAGATCAGATGCACTTACAGGGCCGGGGCCTGTTCCTTCGCCGTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1636

Amino Acids

177.68

Weight (kDa)

8.44

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 152 - 272 4.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 155 - 275 3.9e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 155 - 277 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 161 - 274 5.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AarI CACCTGC 1 cut(s) 3269
Acc36I ACCTGC 5 cut(s) 883, 2520, 3269, 3283, 3772
AccB1I GGYRCC 3 cut(s) 50, 436, 3164
AccI GTMKAC 1 cut(s) 181
AccII CGCG 4 cut(s) 44, 46, 949, 4365
AcoI YGGCCR 3 cut(s) 217, 2586, 4438
AcsI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2723, 3443, 4168, 4802
AcuI CTGAAG 8 cut(s) 732, 1355, 1366, 2033, 3300, 3456, 4412, 4430
AcvI CACGTG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 2914
AfaI GTAC 6 cut(s) 250, 342, 670, 1535, 2046, 3792
AfeI AGCGCT 1 cut(s) 993
AflII CTTAAG 1 cut(s) 2980
AflIII ACRYGT 4 cut(s) 37, 821, 823, 4674
AgeI ACCGGT 2 cut(s) 2370, 2778
AjiI CACGTC 1 cut(s) 40
Alw21I GWGCWC 2 cut(s) 2362, 4402
Alw26I GTCTC 6 cut(s) 694, 1014, 2577, 2626, 2831, 3528
Alw44I GTGCAC 1 cut(s) 2358
AlwNI CAGNNNCTG 1 cut(s) 1109
Ama87I CYCGRG 1 cut(s) 1836
Aor51HI AGCGCT 1 cut(s) 993
AoxI GGCC 8 cut(s) 96, 217, 2252, 2586, 4438, 4459, 4877, 4883
ApaLI GTGCAC 1 cut(s) 2358
ApoI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2723, 3443, 4168, 4802
AseI ATTAAT 1 cut(s) 834
AsiGI ACCGGT 2 cut(s) 2370, 2778
Asp700I GAANNNNTTC 4 cut(s) 1882, 2070, 2460, 4335
AspLEI GCGC 4 cut(s) 46, 994, 2733, 2991
AsuC2I CCSGG 1 cut(s) 4881
AsuHPI GGTGA 9 cut(s) 291, 620, 668, 1355, 3148, 3296, 3617, 3743, 4200
AsuII TTCGAA 1 cut(s) 4555
AvaI CYCGRG 1 cut(s) 1836
AvaII GGWCC 7 cut(s) 1688, 1841, 2639, 3053, 3655, 3815, 4035
BaeGI GKGCMC 6 cut(s) 53, 439, 1206, 1285, 2362, 4156
BaeI ACNNNNGTAYC 4 cut(s) 324, 357, 4263, 4296
BalI TGGCCA 2 cut(s) 219, 2588
BamHI GGATCC 2 cut(s) 1097, 3647
BanI GGYRCC 3 cut(s) 50, 436, 3164
BanII GRGCYC 1 cut(s) 2451
BauI CACGAG 2 cut(s) 211, 3711
BbrPI CACGTG 1 cut(s) 824
BbsI GAAGAC 4 cut(s) 824, 1455, 3296, 4728
Bbv12I GWGCWC 2 cut(s) 2362, 4402
BbvCI CCTCAGC 1 cut(s) 1102
BceAI ACGGC 4 cut(s) 92, 3429, 4740, 4882
BcgI CGANNNNNNTGC 4 cut(s) 109, 122, 143, 156
BciVI GTATCC 3 cut(s) 1952, 4291, 4766
BclI TGATCA 1 cut(s) 3187
BcnI CCSGG 1 cut(s) 4881
BcoDI GTCTC 6 cut(s) 694, 1014, 2577, 2626, 2831, 3528
BfaI CTAG 7 cut(s) 705, 1050, 2132, 2736, 4346, 4464, 4499
BfmI CTRYAG 5 cut(s) 369, 1543, 2280, 3276, 4191
BfoI RGCGCY 1 cut(s) 995
BfrI CTTAAG 1 cut(s) 2980
BfuAI ACCTGC 5 cut(s) 883, 2520, 3269, 3283, 3772
BfuI GTATCC 3 cut(s) 1952, 4291, 4766
BglII AGATCT 3 cut(s) 2128, 2611, 3979
Bme18I GGWCC 7 cut(s) 1688, 1841, 2639, 3053, 3655, 3815, 4035
BmeT110I CYCGRG 1 cut(s) 1836
BmgBI CACGTC 1 cut(s) 40
BmrI ACTGGG 4 cut(s) 2895, 3085, 4040, 4726
BmuI ACTGGG 4 cut(s) 2895, 3085, 4040, 4726
BpiI GAAGAC 4 cut(s) 824, 1455, 3296, 4728
BpmI CTGGAG 7 cut(s) 47, 633, 1388, 1488, 1752, 3669, 4296
Bpu10I CCTNAGC 3 cut(s) 1102, 3659, 4658
Bpu14I TTCGAA 1 cut(s) 4555
BpuEI CTTGAG 4 cut(s) 2616, 2991, 3990, 4310
BpuMI CCSGG 1 cut(s) 4881
BsaAI YACGTR 1 cut(s) 824
BsaHI GRCGYC 1 cut(s) 2914
BsaI GGTCTC 3 cut(s) 694, 2577, 2831
BsaWI WCCGGW 2 cut(s) 2370, 2778
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 2953, 2983
Bse118I RCCGGY 3 cut(s) 2370, 2778, 4440
Bse3DI GCAATG 1 cut(s) 1227
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 9 cut(s) 294, 687, 1116, 2344, 2948, 3024, 3932, 4115, 4148
BseRI GAGGAG 4 cut(s) 942, 2441, 2974, 3506
BseSI GKGCMC 6 cut(s) 53, 439, 1206, 1285, 2362, 4156
BseYI CCCAGC 1 cut(s) 3623
BsgI GTGCAG 3 cut(s) 38, 2241, 3486
Bsh1236I CGCG 4 cut(s) 44, 46, 949, 4365
BshFI GGCC 8 cut(s) 98, 219, 2254, 2588, 4440, 4461, 4879, 4885
BshNI GGYRCC 3 cut(s) 50, 436, 3164
BshTI ACCGGT 2 cut(s) 2370, 2778
BsiHKAI GWGCWC 2 cut(s) 2362, 4402
BsiHKCI CYCGRG 1 cut(s) 1836
BsiSI CCGG 5 cut(s) 2371, 2779, 2785, 4441, 4880
BslFI GGGAC 3 cut(s) 192, 430, 1674
BsmAI GTCTC 6 cut(s) 694, 1014, 2577, 2626, 2831, 3528
BsmFI GGGAC 3 cut(s) 192, 430, 1674
BsmI GAATGC 2 cut(s) 295, 4161
BsnI GGCC 8 cut(s) 98, 219, 2254, 2588, 4440, 4461, 4879, 4885
Bso31I GGTCTC 3 cut(s) 694, 2577, 2831
BsoBI CYCGRG 1 cut(s) 1836
Bsp119I TTCGAA 1 cut(s) 4555
Bsp1286I GDGCHC 8 cut(s) 53, 439, 1206, 1285, 2362, 2451, 4156, 4402
Bsp19I CCATGG 1 cut(s) 4456
BspANI GGCC 8 cut(s) 98, 219, 2254, 2588, 4440, 4461, 4879, 4885
BspCNI CTCAG 9 cut(s) 295, 688, 1115, 2343, 2949, 3025, 3931, 4114, 4147
BspFNI CGCG 4 cut(s) 44, 46, 949, 4365
BspMAI CTGCAG 2 cut(s) 2284, 3280
BspMI ACCTGC 5 cut(s) 883, 2520, 3269, 3283, 3772
BspT104I TTCGAA 1 cut(s) 4555
BspT107I GGYRCC 3 cut(s) 50, 436, 3164
BspTI CTTAAG 1 cut(s) 2980
BspTNI GGTCTC 3 cut(s) 694, 2577, 2831
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 3 cut(s) 2370, 2778, 4440
BssAI RCCGGY 3 cut(s) 2370, 2778, 4440
BssNAI GTATAC 1 cut(s) 182
BssNI GRCGYC 1 cut(s) 2914
BssSI CACGAG 2 cut(s) 211, 3711
BssT1I CCWWGG 4 cut(s) 2567, 2747, 4156, 4456
Bst1107I GTATAC 1 cut(s) 182
Bst2BI CACGAG 2 cut(s) 211, 3711
Bst6I CTCTTC 6 cut(s) 810, 2076, 2466, 3319, 4428, 4530
BstACI GRCGYC 1 cut(s) 2914
BstAFI CTTAAG 1 cut(s) 2980
BstAPI GCANNNNNTGC 2 cut(s) 4475, 4836
BstBAI YACGTR 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 4555
BstC8I GCNNGC 9 cut(s) 487, 858, 2256, 2382, 2555, 3619, 3783, 4442, 4504
BstDSI CCRYGG 3 cut(s) 3411, 4243, 4456
BstENI CCTNNNNNAGG 1 cut(s) 3792
BstFNI CGCG 4 cut(s) 44, 46, 949, 4365
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 4 cut(s) 46, 994, 2733, 2991
BstMAI GTCTC 6 cut(s) 694, 1014, 2577, 2626, 2831, 3528
BstNSI RCATGY 3 cut(s) 489, 2805, 4678
BstSFI CTRYAG 5 cut(s) 369, 1543, 2280, 3276, 4191
BstSLI GKGCMC 6 cut(s) 53, 439, 1206, 1285, 2362, 4156
BstUI CGCG 4 cut(s) 44, 46, 949, 4365
BstV2I GAAGAC 4 cut(s) 824, 1455, 3296, 4728
BstX2I RGATCY 6 cut(s) 1097, 2128, 2611, 2976, 3647, 3979
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 6 cut(s) 1097, 2128, 2611, 2976, 3647, 3979
BstZ17I GTATAC 1 cut(s) 182
BsuI GTATCC 3 cut(s) 1952, 4291, 4766
BsuRI GGCC 8 cut(s) 98, 219, 2254, 2588, 4440, 4461, 4879, 4885
BtgI CCRYGG 3 cut(s) 3411, 4243, 4456
BtgZI GCGATG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 40
BtsI GCAGTG 3 cut(s) 2277, 3490, 3872
BveI ACCTGC 5 cut(s) 883, 2520, 3269, 3283, 3772
Cac8I GCNNGC 9 cut(s) 487, 858, 2256, 2382, 2555, 3619, 3783, 4442, 4504
CaiI CAGNNNCTG 1 cut(s) 1109
CfoI GCGC 4 cut(s) 46, 994, 2733, 2991
Cfr10I RCCGGY 3 cut(s) 2370, 2778, 4440
CseI GACGC 4 cut(s) 1095, 2903, 4214, 4319
CsiI ACCWGGT 2 cut(s) 3793, 3951
Csp6I GTAC 6 cut(s) 249, 341, 669, 1534, 2045, 3791
CspAI ACCGGT 2 cut(s) 2370, 2778
CviQI GTAC 6 cut(s) 249, 341, 669, 1534, 2045, 3791
DraI TTTAAA 1 cut(s) 3598
EaeI YGGCCR 3 cut(s) 217, 2586, 4438
Eam1104I CTCTTC 6 cut(s) 810, 2076, 2466, 3319, 4428, 4530
EarI CTCTTC 6 cut(s) 810, 2076, 2466, 3319, 4428, 4530
EciI GGCGGA 1 cut(s) 3712
Eco130I CCWWGG 4 cut(s) 2567, 2747, 4156, 4456
Eco24I GRGCYC 1 cut(s) 2451
Eco31I GGTCTC 3 cut(s) 694, 2577, 2831
Eco32I GATATC 2 cut(s) 175, 4375
Eco47I GGWCC 7 cut(s) 1688, 1841, 2639, 3053, 3655, 3815, 4035
Eco47III AGCGCT 1 cut(s) 993
Eco57I CTGAAG 8 cut(s) 732, 1355, 1366, 2033, 3300, 3456, 4412, 4430
Eco72I CACGTG 1 cut(s) 824
Eco88I CYCGRG 1 cut(s) 1836
EcoNI CCTNNNNNAGG 1 cut(s) 3792
EcoO109I RGGNCCY 5 cut(s) 97, 3053, 3655, 4035, 4883
EcoRI GAATTC 1 cut(s) 4168
EcoRV GATATC 2 cut(s) 175, 4375
EcoT14I CCWWGG 4 cut(s) 2567, 2747, 4156, 4456
EcoT22I ATGCAT 1 cut(s) 2803
EcoT38I GRGCYC 1 cut(s) 2451
ErhI CCWWGG 4 cut(s) 2567, 2747, 4156, 4456
FaqI GGGAC 3 cut(s) 192, 430, 1674
FauI CCCGC 2 cut(s) 39, 4049
FauNDI CATATG 1 cut(s) 1194
FbaI TGATCA 1 cut(s) 3187
FblI GTMKAC 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 2451
FspBI CTAG 7 cut(s) 705, 1050, 2132, 2736, 4346, 4464, 4499
GlaI GCGC 4 cut(s) 45, 993, 2732, 2990
GsaI CCCAGC 1 cut(s) 3627
GsuI CTGGAG 7 cut(s) 47, 633, 1388, 1488, 1752, 3669, 4296
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 8 cut(s) 98, 219, 2254, 2588, 4440, 4461, 4879, 4885
HapII CCGG 5 cut(s) 2371, 2779, 2785, 4441, 4880
HgaI GACGC 4 cut(s) 1095, 2903, 4214, 4319
HhaI GCGC 4 cut(s) 46, 994, 2733, 2991
Hin1I GRCGYC 1 cut(s) 2914
Hin6I GCGC 4 cut(s) 44, 992, 2731, 2989
HinP1I GCGC 4 cut(s) 44, 992, 2731, 2989
HincII GTYRAC 4 cut(s) 147, 1902, 4672, 4764
HindII GTYRAC 4 cut(s) 147, 1902, 4672, 4764
HindIII AAGCTT 5 cut(s) 599, 1124, 2504, 3264, 4332
HpaII CCGG 5 cut(s) 2371, 2779, 2785, 4441, 4880
HphI GGTGA 9 cut(s) 291, 620, 668, 1355, 3148, 3296, 3617, 3743, 4200
Hpy99I CGWCG 3 cut(s) 44, 113, 134
HpyCH4IV ACGT 2 cut(s) 39, 823
HpySE526I ACGT 2 cut(s) 39, 823
Hsp92I GRCGYC 1 cut(s) 2914
HspAI GCGC 4 cut(s) 44, 992, 2731, 2989
KroI GCCGGC 1 cut(s) 4440
KroNI GCCGGC 1 cut(s) 4442
Ksp22I TGATCA 1 cut(s) 3187
MabI ACCWGGT 2 cut(s) 3793, 3951
MaeI CTAG 7 cut(s) 705, 1050, 2132, 2736, 4346, 4464, 4499
MaeII ACGT 2 cut(s) 39, 823
MaeIII GTNAC 8 cut(s) 403, 774, 781, 787, 3319, 3516, 4417, 4784
MfeI CAATTG 2 cut(s) 882, 3543
MflI RGATCY 6 cut(s) 1097, 2128, 2611, 2976, 3647, 3979
MhlI GDGCHC 8 cut(s) 53, 439, 1206, 1285, 2362, 2451, 4156, 4402
MlsI TGGCCA 2 cut(s) 219, 2588
MluNI TGGCCA 2 cut(s) 219, 2588
MlyI GAGTC 5 cut(s) 1907, 2998, 3305, 4093, 4461
MmeI TCCRAC 4 cut(s) 294, 388, 2783, 4722
Mox20I TGGCCA 2 cut(s) 219, 2588
Mph1103I ATGCAT 1 cut(s) 2803
MroNI GCCGGC 1 cut(s) 4440
MroXI GAANNNNTTC 4 cut(s) 1882, 2070, 2460, 4335
MscI TGGCCA 2 cut(s) 219, 2588
MslI CAYNNNNRTG 7 cut(s) 243, 558, 640, 1007, 2201, 2242, 3195
Msp20I TGGCCA 2 cut(s) 219, 2588
MspA1I CMGCKG 3 cut(s) 3470, 3812, 4253
MspCI CTTAAG 1 cut(s) 2980
MspI CCGG 5 cut(s) 2371, 2779, 2785, 4441, 4880
MunI CAATTG 2 cut(s) 882, 3543
Mva1269I GAATGC 2 cut(s) 295, 4161
MvnI CGCG 4 cut(s) 44, 46, 949, 4365
NaeI GCCGGC 1 cut(s) 4442
NciI CCSGG 1 cut(s) 4881
NcoI CCATGG 1 cut(s) 4456
NdeI CATATG 1 cut(s) 1194
NgoMIV GCCGGC 1 cut(s) 4440
NmeAIII GCCGAG 2 cut(s) 1273, 3157
NmuCI GTSAC 3 cut(s) 3319, 4417, 4784
NsiI ATGCAT 1 cut(s) 2803
NspI RCATGY 3 cut(s) 489, 2805, 4678
NspV TTCGAA 1 cut(s) 4555
PaeI GCATGC 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 3269
PciI ACATGT 1 cut(s) 4674
PctI GAATGC 2 cut(s) 295, 4161
PdiI GCCGGC 1 cut(s) 4442
PdmI GAANNNNTTC 4 cut(s) 1882, 2070, 2460, 4335
PfoI TCCNGGA 2 cut(s) 3883, 4228
PinAI ACCGGT 2 cut(s) 2370, 2778
PleI GAGTC 5 cut(s) 1906, 2998, 3305, 4093, 4460
PmaCI CACGTG 1 cut(s) 824
PmlI CACGTG 1 cut(s) 824
PpsI GAGTC 5 cut(s) 1906, 2998, 3305, 4093, 4460
Ppu21I YACGTR 1 cut(s) 824
PpuMI RGGWCCY 3 cut(s) 3053, 3655, 4035
PscI ACATGT 1 cut(s) 4674
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 3 cut(s) 3053, 3655, 4035
PspCI CACGTG 1 cut(s) 824
PspFI CCCAGC 1 cut(s) 3623
PspPPI RGGWCCY 3 cut(s) 3053, 3655, 4035
PsrI GAACNNNNNNTAC 2 cut(s) 2037, 2069
PstI CTGCAG 2 cut(s) 2284, 3280
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 6 cut(s) 1097, 2128, 2611, 2976, 3647, 3979
PvuII CAGCTG 3 cut(s) 3470, 3812, 4253
RsaI GTAC 6 cut(s) 250, 342, 670, 1535, 2046, 3792
RsaNI GTAC 6 cut(s) 249, 341, 669, 1534, 2045, 3791
RseI CAYNNNNRTG 7 cut(s) 243, 558, 640, 1007, 2201, 2242, 3195
SbfI CCTGCAGG 1 cut(s) 3280
SchI GAGTC 5 cut(s) 1907, 2998, 3305, 4093, 4461
SdaI CCTGCAGG 1 cut(s) 3280
SduI GDGCHC 8 cut(s) 53, 439, 1206, 1285, 2362, 2451, 4156, 4402
SexAI ACCWGGT 2 cut(s) 3793, 3951
SfcI CTRYAG 5 cut(s) 369, 1543, 2280, 3276, 4191
SfuI TTCGAA 1 cut(s) 4555
SinI GGWCC 7 cut(s) 1688, 1841, 2639, 3053, 3655, 3815, 4035
SmiMI CAYNNNNRTG 7 cut(s) 243, 558, 640, 1007, 2201, 2242, 3195
SmlI CTYRAG 5 cut(s) 2631, 2980, 3006, 4005, 4289
SmoI CTYRAG 5 cut(s) 2631, 2980, 3006, 4005, 4289
SphI GCATGC 1 cut(s) 489
Sse8387I CCTGCAGG 1 cut(s) 3280
SspI AATATT 2 cut(s) 1152, 2094
SspMI CTAG 7 cut(s) 705, 1050, 2132, 2736, 4346, 4464, 4499
StyI CCWWGG 4 cut(s) 2567, 2747, 4156, 4456
TaiI ACGT 2 cut(s) 42, 826
TaqI TCGA 9 cut(s) 27, 132, 538, 585, 1669, 1876, 2454, 2874, 4555
TatI WGTACW 2 cut(s) 340, 2044
TauI GCSGC 5 cut(s) 77, 80, 1043, 2914, 4745
TseFI GTSAC 3 cut(s) 3319, 4417, 4784
Tsp45I GTSAC 3 cut(s) 3319, 4417, 4784
TspGWI ACGGA 1 cut(s) 3953
Vha464I CTTAAG 1 cut(s) 2980
VneI GTGCAC 1 cut(s) 2358
VpaK11BI GGWCC 7 cut(s) 1688, 1841, 2639, 3053, 3655, 3815, 4035
VspI ATTAAT 1 cut(s) 834
XagI CCTNNNNNAGG 1 cut(s) 3792
XapI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2723, 3443, 4168, 4802
XbaI TCTAGA 2 cut(s) 2131, 2735
XceI RCATGY 3 cut(s) 489, 2805, 4678
XcmI CCANNNNNNNNNTGG 2 cut(s) 639, 982
XmiI GTMKAC 1 cut(s) 181
XmnI GAANNNNTTC 4 cut(s) 1882, 2070, 2460, 4335
XspI CTAG 7 cut(s) 705, 1050, 2132, 2736, 4346, 4464, 4499
Zsp2I ATGCAT 1 cut(s) 2803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.