MD02G1168900.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008489
Physical Location & Seq
Forward (+)
14471942 .. 14484977
13036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1168900.v1.1.491

Sequence Viewer

Length: 4929 bp
ATGGAGTGGACGACGGTGCAGCACCTGGATCTACGACATGTCGGACGCAGCTCAAAACCGTTGCAGCCTCATGCTGCTGCCTTTCATCCCCATCAGGCCCTCGTTGCCGTCGCCATTGGAAACTACATCATTGAAATGGATGCGTTGACAGGAAGCAAGATTTCATCTATTGATATTGGTACACCCATTGTCCGCATGTCTTATAGCCCCACAAGTGGGCATTCTGTGGTGGCGATCCTTGAGGACTGTACCATTCGCTCTTGTGATTTTGATGCTGAGCAAACATGCGTCTTGCATTCACCTGAGAAGAAGACAGAACAAATTTCTTCTGATACAGAAGTTCATCTAGCTTTGACGTCTCTCCAGCCTGTAGTATTTTTCGGTTTCCACAAGAAAATGAGTGTCACAGTTGTTGGAACCGTTGAAGGAGGAAGAGCGCCGACAAAAATAAAGACAGACTTGAAGAAACCGATTGTCAATCTTGCCTGCCACCCTCGCCTCCCTGTCCTGTATGTGGCTTATGCTGATGGTCTGATTCGAGCCTACAACATTCACACATATGCGGTTCATTACACCTTACAAATCGATAACACCATAAAGCTTATTGGTGCTGGTGCATTTGGTTTTCACCCAACATTGGAGTGGATATTTGTTGGTGATAGACGGGGTACGCTGCTTGCATGGGATGTGTCAACTGAGAGACCTAGCATGATTGGCATTACACAAGTGGGTTCCCAACCAATAGCTTCAGTTTCCTGGCTCCCAATGTTGCGGTTACTGGTAACTGTGTCCAAGGATGGAACTCTTCAAGTGTGGAAAACACGGGTTATAATTAATCCTAATAGACCTCCAATGCAAGCAAATTTCTTTGAGCCTGCTGCAATTGAATCACTTGATATCCCTCGCATCCTTTCTCAGCAGGGTGGAGAAGCAGCTTACCCTTTACCGCGAATTAAAACTCTAGAAGTTCACTCGAAATTAAATTTAGCAGCACTTCTGTTTGCAAATATGACAGGTGGAGACAATGTGAAAAATCGGGCTGCATACACTAGGGAAGGAAGGAAACAACTGTTTGCAGTTTTGCAAGGTGCAAGAGGATCTTCAGCATCTGTTTTGAAAGAAAAGCTTTCAGCTTTGGGTTCATCTGGAATATTAGCCGAACATCAACTTCAAGCTCAACTGCAAGAACATCATTCGAAAGGCCGCAGTCAGCTTACAATATCAGACATTGCTCGGAAGGCTTTTCTCCACAGTCATTTTATGGAAGGTCATGCCAAAAGTGCCCCAATATCTCGGCTGCCCCTCATTACTATTATAGACACTAAGCATCATCTGAAGGACTTTCCTGTATTTCAGCCATTTCACCTGGAGTTAAATTTCTTCAATAAAGAGAACCGGGTTCTTCATTATCCTGTCAGGGCCTTTTATGTTGATGGATTACACCTTATGGCATATAACATTTGTTCTGGAGCAGATAGCATTTACAAAAAACTTTACACAACGGTTCCTGGAAATGTGGAATATCATCCAAAGTACATGGCCTACGGTAAAAAACAGGGTCTATTTCTTGTTGTCTATGAATTTAGTGGTGCTACAAATGAAGTGGTGCTTTATTTTGAAAATACCAATACTCAGGCAGCAAACAGCAAGTGTACCACAATAAAAGGTCGTGATGCAGCATTTATTGGACCTAATGAAAATCAGTTTGCAATTCTTGATGATGATAAGACTGGACTGGTTTTGTATATTCTGCCTAAAAAGGCTTCTCCTGAAGCTAATGAGAAGAATCTATTGGCTGAGGAAAGCCAACCTGTGGATACTGACGCTGGTCCCAAGGGTCCAATGCAATTTATGTTTGAAAGTGAAGTGGATCGAATTTTTTCGACTCCAATAGAGTCAACATTGATGTTTGCTTCTCATGGGAGTCAGATTGGCTTGGCAAAGTTGATCCAGGGATACCGGCTTTCCAATTCTGGTGGTCATTATATAGCAACAAAGGGTGAAGGGAAAAACACAATTAAGTTGAAACTAAATGAGATTGTACTTCAGGTTCACTGGCAAGAAACTCTTAGGGGCTATGTAGCTGGAATACTAACAACGCACAGGGTGCTTATAGTTTCGGCAGATCTAGATATACTGGCAGGCGGTTCCGCAAAATTTGATAAAGGACTTCCTTCGATATCCTTTACAACATTTTGGTGTTGTTACAACTTGTTTGAGCACTTGACCTCTATTCACTTTAGATCACTTTTATGGGTTGGGCCTGCACTTCTCTTTTCTACTACCACTGCAATTAGCGTGCTTGGCTGGGACGGGAAAGTAAGGGCTATTCTCTCTATCAGTATGCCTTATGCAGTTTTGGTTGGTGCGCTGAATGATCGGTTGTTGCTTGCTACCCCAACAGAAATAAACCCCAGACAAAGGAAGGGAGTTGAGATTAAGAGCTGTCTTGTTGGGCTCCTTGAACCTCTTCTTATTGGATTTGCCACAATGCAAGAGAGATTTGAGCAGAAACTTGACCTTCCAGAGATATTATATCAAATAACATCAAGGTTTGACAGCCTGCGTATCACTCCAAGGTCTCTTGATATTCTTGCTAGAGGCTCTCCTGTTTGTGGAGATCTTTCTGTCTCACTATCTCAAGCTGGACCCCAGTTTACCCAGGTGTTGAGGGGTGTCTATGCCATCAAAGCTCTCCGTTTTTCGACTGCTTTGTCTGTCTTAAAAGACGAATTTCTGCGCTCCAGAGATTATCCAAGATGTCCTCCAACCTCTCATTTGTTCCACCGGTTCCACCAGTTGGGTTATGCATGTATCAAGTTTGGGCAGTTTGATAGTGCAAAAGAAACCTTTGAAGTTATAGGTGATTATGAAAGTATGCTCGACCTCTTTATATGCCACCTAAACCCCAGTGCCATGCGGCGTCTTGCTCAAAAACTGGAAGAAGATGGCACAGATTCAGAATTAAGACGATACTGTGAAAGGATTTTAAGAGCCCGTTCTACTGGATGGACTCAAGGAATTTTTGCCAACTTTGCCGCTGAGAGTATGGTCCCTAAAGGTCCTGAATGGGGTGGTGGGAACTGGGAAATCAAAACCCCAACAAACATGAAGGCTATACCTCAGTGGGAACTGGCTGCAGAGGTGATGCCGTACATGAAGACTGACGATGGCACCATTCCATCCATTATCGCTGATCATATTGGTGTTTACTTGGGATCAATCAAAGGAAGAGGCAACATTGTAGAAGTAAGGGAAGATAGCTTGGTCAAAGCTTTTAAATCAGCAGGTGGTGGCAACAAGCCAAATGGTCTTCCGTTGTCTACATCCACATCTAATATGTTCAAGGGAGTGCCTGCTGGTGATTCGCTAATGGGTCTGGAAACACTTAACAACAAACAATTTGCCAGTTCCTCTGCAGCAGATGAACAGGCTAAAGCTGAAGAAGAATTCAAGAAAACAATGTACGGGGCAGCTGATGGTAGCAGCAGTGATGAAGAGGGAACTTCAAAAGCTAAAAAGTTACACATTCGAATAAGAGACAAGCCAATTGCTTCTACTGCAGTGGACGTTGATAAAATCAAAGAAGCCACAAAGCAGCTCAAACTCGGTGAAGGGTTGGGTCCACCCATGACCAGGACCAAGTCATTGACTATTGGATCCCAGGACCTTAGCCAGATGTTATCCCAACCTCCTCCTCCAGCTAACGGTGGGTCAGTGGCTCCTCGTGTAGGCTCTGCCCCTGGTGATCTATTTGGTATGGACTCGTTTACACAACCTGCAACAGTATCACATCAAGCTCCTACCTCAACAGGTAAGGGGGTTGGTCCTGCGCCGATTCCAGAGGACTTTTTCCAGAATACGATACCATCTCTTCAGGTTGCGGCCGCACTGCCCCCTCCAGGGACCTATCTTTCAAAAATGGATCAAGCTTCTCAAGGGTTTGAAAGCAACAAGGAAGCATTTAACCAAGCCAATGCATCCAATGCCAATGTTCGTCTTCCTGATGCTGGTGTTCCCCCTCAAGCTAGTCAGCCGGCTGCTGCTCCATTCGAGCCCGTTGGACTTCCTGATGGTGGTGTCCCACCATCCTCAGGCCAGGTTGCCGCGCAGCATCAATCTCATATTCAATCAACTCAGTTCCCAGTTTCTACTCAACCTCTTGATCTGAGTGTCCTTGGGGTTCCAACTTCTGCTGACTCTGGAAAACCTTCAGCGCAGCCTTCTTCTCCACCATCTTCTGTGCGTCCTGGACAGGTACCCCGTGGGGCTGCTGCTTCTGTATGTTTTAAGACTGGAGTTGCCCACCTTGAGCAGAATCAACTTTCAGATGCGTTGTCTTGCTTTGATGAAGCTTTTCTTGCACTGGCTAAGGATCAGTCTCGTGGAGCTGATATTAAAGCACAAGGGACCATCTGTGCTCAATACAAAATAGCAGTCACTCTTCTTCGGGAAATTGGGCGGCTGCAAAGAGTTCAAGGTCCTAGTGCAATCAGTGCAAAAGACGAGATGGCCAGACTCTCACGCCACCTGGGTTCTTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATCCGAACTGCAATTAAGCGGAACATGGAGGTGCAGAATTATGCTTATTCGAAGCAGATGCTTGAACTCCTGCTATCCAAAGCACCTCCAAGTAAGCAGGAAGAATTGAGGAGCTTAGTTGATATGTGTGTGCAAAGGGGTCTGTCCAATAAATCCATTGATCCACTTGAAGATCCTTCCCAGTTCTGTGCCGCCACACTTAGTCGTCTGTCAACTATTGGATATGACGTTTGTGATCTTTGTGGGGCCAAATTTTCTGCTCTCTCATCACCCGGTTGCATTATATGCGGTATGGGAAGCATTAAAAGATCAGATGCACTCACAGGGCCAGGGCCTGTTCCTTCACCGTTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1643

Amino Acids

178.48

Weight (kDa)

8.57

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 20 - 272 1.3e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 152 - 274 3.6e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 155 - 277 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 161 - 274 8.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AarI CACCTGC 1 cut(s) 3278
AasI GACNNNNNNGTC 1 cut(s) 2713
AatII GACGTC 1 cut(s) 359
Acc36I ACCTGC 2 cut(s) 3278, 3787
Acc65I GGTACC 1 cut(s) 4258
AccB1I GGYRCC 2 cut(s) 3173, 4258
AccB7I CCANNNNNTGG 2 cut(s) 1813, 2800
AccI GTMKAC 1 cut(s) 3323
AccII CGCG 2 cut(s) 949, 4109
AcoI YGGCCR 2 cut(s) 3885, 4512
AcuI CTGAAG 8 cut(s) 732, 1086, 1355, 1791, 2030, 3462, 3860, 4197
AcyI GRCGYC 2 cut(s) 356, 2923
AdeI CACNNNGTG 1 cut(s) 2107
AfaI GTAC 9 cut(s) 181, 250, 670, 1535, 1654, 2043, 3155, 3467, 4260
AflIII ACRYGT 1 cut(s) 37
AgeI ACCGGT 1 cut(s) 2787
AjuI GAANNNNNNNTTGG 2 cut(s) 1775, 1807
Alw21I GWGCWC 2 cut(s) 2223, 4423
Alw26I GTCTC 7 cut(s) 363, 694, 1014, 2586, 2635, 3534, 4386
AlwNI CAGNNNCTG 3 cut(s) 25, 1109, 4907
ArsI GACNNNNNNTTYG 2 cut(s) 4422, 4454
AseI ATTAAT 1 cut(s) 834
AsiGI ACCGGT 1 cut(s) 2787
Asp700I GAANNNNTTC 4 cut(s) 1879, 2469, 4210, 4356
Asp718I GGTACC 1 cut(s) 4258
AspLEI GCGC 6 cut(s) 439, 2371, 2742, 3835, 4111, 4219
AsuC2I CCSGG 2 cut(s) 1397, 4845
AsuII TTCGAA 3 cut(s) 1196, 3532, 4622
AxyI CCTNAGG 1 cut(s) 4093
BaeGI GKGCMC 1 cut(s) 1285
BaeI ACNNNNGTAYC 2 cut(s) 324, 357
BalI TGGCCA 1 cut(s) 4514
BamHI GGATCC 1 cut(s) 3659
BanI GGYRCC 2 cut(s) 3173, 4258
BanII GRGCYC 3 cut(s) 2460, 2998, 4059
BauI CACGAG 2 cut(s) 3726, 4383
BbsI GAAGAC 4 cut(s) 317, 3167, 3305, 3992
Bbv12I GWGCWC 2 cut(s) 2223, 4423
BbvCI CCTCAGC 1 cut(s) 1797
BceAI ACGGC 2 cut(s) 92, 3136
BcgI CGANNNNNNTGC 2 cut(s) 4612, 4646
BciVI GTATCC 3 cut(s) 1810, 1949, 4583
BclI TGATCA 1 cut(s) 3196
BcnI CCSGG 2 cut(s) 1397, 4845
BcoDI GTCTC 7 cut(s) 363, 694, 1014, 2586, 2635, 3534, 4386
BfaI CTAG 8 cut(s) 347, 705, 962, 1050, 2129, 2598, 4029, 4485
BfmI CTRYAG 4 cut(s) 369, 3138, 3417, 3561
BfoI RGCGCY 1 cut(s) 440
BfuAI ACCTGC 2 cut(s) 3278, 3787
BfuI GTATCC 3 cut(s) 1810, 1949, 4583
BglII AGATCT 2 cut(s) 2125, 2620
BlpI GCTNAGC 1 cut(s) 276
BmrI ACTGGG 5 cut(s) 2647, 2904, 3094, 4139, 4747
BmuI ACTGGG 5 cut(s) 2647, 2904, 3094, 4139, 4747
BoxI GACNNNNGTC 1 cut(s) 1827
BpiI GAAGAC 4 cut(s) 317, 3167, 3305, 3992
BpmI CTGGAG 7 cut(s) 347, 1388, 1488, 2728, 3684, 3885, 4317
Bpu10I CCTNAGC 3 cut(s) 1797, 3671, 4371
Bpu1102I GCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 3 cut(s) 1196, 3532, 4622
BpuEI CTTGAG 6 cut(s) 260, 2625, 3000, 3921, 4008, 4331
BpuMI CCSGG 2 cut(s) 1397, 4845
Bsa29I ATCGAT 1 cut(s) 585
BsaHI GRCGYC 2 cut(s) 356, 2923
BsaI GGTCTC 2 cut(s) 694, 2586
BsaWI WCCGGW 1 cut(s) 2787
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bse118I RCCGGY 3 cut(s) 1959, 2787, 4036
Bse21I CCTNAGG 1 cut(s) 4093
Bse3DI GCAATG 1 cut(s) 1227
BseCI ATCGAT 1 cut(s) 585
BseMI GCAATG 1 cut(s) 1227
BseRI GAGGAG 4 cut(s) 3684, 3687, 3714, 4696
BseSI GKGCMC 1 cut(s) 1285
BseX3I CGGCCG 1 cut(s) 3885
BseYI CCCAGC 1 cut(s) 2307
BsgI GTGCAG 3 cut(s) 38, 2250, 4625
Bsh1236I CGCG 2 cut(s) 949, 4109
Bsh1285I CGRYCG 1 cut(s) 3888
BshNI GGYRCC 2 cut(s) 3173, 4258
BshTI ACCGGT 1 cut(s) 2787
BshVI ATCGAT 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 3888
BsiHKAI GWGCWC 2 cut(s) 2223, 4423
BsiSI CCGG 5 cut(s) 1396, 1960, 2788, 4037, 4845
BslFI GGGAC 6 cut(s) 1815, 2324, 3038, 3919, 4067, 4423
BsmAI GTCTC 7 cut(s) 363, 694, 1014, 2586, 2635, 3534, 4386
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 6 cut(s) 1815, 2324, 3038, 3919, 4067, 4423
BsmI GAATGC 2 cut(s) 220, 295
Bso31I GGTCTC 2 cut(s) 694, 2586
Bsp119I TTCGAA 3 cut(s) 1196, 3532, 4622
Bsp1286I GDGCHC 6 cut(s) 1285, 2223, 2460, 2998, 4059, 4423
Bsp1720I GCTNAGC 1 cut(s) 276
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 2 cut(s) 949, 4109
BspMAI CTGCAG 3 cut(s) 3142, 3421, 3565
BspMI ACCTGC 2 cut(s) 3278, 3787
BspQI GCTCTTC 1 cut(s) 427
BspT104I TTCGAA 3 cut(s) 1196, 3532, 4622
BspT107I GGYRCC 2 cut(s) 3173, 4258
BspTNI GGTCTC 2 cut(s) 694, 2586
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 3 cut(s) 1959, 2787, 4036
BssAI RCCGGY 3 cut(s) 1959, 2787, 4036
BssNI GRCGYC 2 cut(s) 356, 2923
BssSI CACGAG 2 cut(s) 3726, 4383
BssT1I CCWWGG 4 cut(s) 792, 1833, 2576, 4177
Bst2BI CACGAG 2 cut(s) 3726, 4383
Bst6I CTCTTC 8 cut(s) 427, 810, 2475, 3226, 3492, 3879, 4449, 4551
BstACI GRCGYC 2 cut(s) 356, 2923
BstAPI GCANNNNNTGC 4 cut(s) 2107, 3986, 4496, 4857
BstBI TTCGAA 3 cut(s) 1196, 3532, 4622
BstDSI CCRYGG 1 cut(s) 4264
BstENI CCTNNNNNAGG 1 cut(s) 3729
BstFNI CGCG 2 cut(s) 949, 4109
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 6 cut(s) 439, 2371, 2742, 3835, 4111, 4219
BstMAI GTCTC 7 cut(s) 363, 694, 1014, 2586, 2635, 3534, 4386
BstMCI CGRYCG 1 cut(s) 3888
BstNSI RCATGY 4 cut(s) 41, 199, 288, 2814
BstPAI GACNNNNGTC 1 cut(s) 1827
BstSFI CTRYAG 4 cut(s) 369, 3138, 3417, 3561
BstSLI GKGCMC 1 cut(s) 1285
BstUI CGCG 2 cut(s) 949, 4109
BstV2I GAAGAC 4 cut(s) 317, 3167, 3305, 3992
BstX2I RGATCY 6 cut(s) 28, 1097, 2125, 2620, 3659, 4744
BstYI RGATCY 6 cut(s) 28, 1097, 2125, 2620, 3659, 4744
BstZI CGGCCG 1 cut(s) 3885
Bsu15I ATCGAT 1 cut(s) 585
Bsu36I CCTNAGG 1 cut(s) 4093
BsuI GTATCC 3 cut(s) 1810, 1949, 4583
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 4264
BtsI GCAGTG 4 cut(s) 2286, 3496, 3570, 3890
BveI ACCTGC 2 cut(s) 3278, 3787
CaiI CAGNNNCTG 3 cut(s) 25, 1109, 4907
CciNI GCGGCCGC 1 cut(s) 3885
CfoI GCGC 6 cut(s) 439, 2371, 2742, 3835, 4111, 4219
Cfr10I RCCGGY 3 cut(s) 1959, 2787, 4036
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 5 cut(s) 54, 277, 1832, 2912, 4235
Csp6I GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3154, 3466, 4259
CspAI ACCGGT 1 cut(s) 2787
CspCI CAANNNNNGTGG 4 cut(s) 1584, 1619, 1957, 1992
CviQI GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3154, 3466, 4259
DraI TTTAAA 1 cut(s) 3280
DraIII CACNNNGTG 1 cut(s) 2107
DrdI GACNNNNNNGTC 1 cut(s) 2713
DseDI GACNNNNNNGTC 1 cut(s) 2713
EaeI YGGCCR 2 cut(s) 3885, 4512
EagI CGGCCG 1 cut(s) 3885
Eam1104I CTCTTC 8 cut(s) 427, 810, 2475, 3226, 3492, 3879, 4449, 4551
EarI CTCTTC 8 cut(s) 427, 810, 2475, 3226, 3492, 3879, 4449, 4551
EclXI CGGCCG 1 cut(s) 3885
Eco130I CCWWGG 4 cut(s) 792, 1833, 2576, 4177
Eco24I GRGCYC 3 cut(s) 2460, 2998, 4059
Eco31I GGTCTC 2 cut(s) 694, 2586
Eco32I GATATC 2 cut(s) 898, 2181
Eco52I CGGCCG 1 cut(s) 3885
Eco57I CTGAAG 8 cut(s) 732, 1086, 1355, 1791, 2030, 3462, 3860, 4197
Eco81I CCTNAGG 1 cut(s) 4093
EcoNI CCTNNNNNAGG 1 cut(s) 3729
EcoO109I RGGNCCY 7 cut(s) 97, 1419, 3062, 3667, 3906, 4481, 4904
EcoRI GAATTC 1 cut(s) 3449
EcoRV GATATC 2 cut(s) 898, 2181
EcoT14I CCWWGG 4 cut(s) 792, 1833, 2576, 4177
EcoT22I ATGCAT 2 cut(s) 2812, 3982
EcoT38I GRGCYC 3 cut(s) 2460, 2998, 4059
ErhI CCWWGG 4 cut(s) 792, 1833, 2576, 4177
Esp3I CGTCTC 1 cut(s) 363
FaqI GGGAC 6 cut(s) 1815, 2324, 3038, 3919, 4067, 4423
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 3196
FblI GTMKAC 1 cut(s) 3323
FriOI GRGCYC 3 cut(s) 2460, 2998, 4059
FspBI CTAG 8 cut(s) 347, 705, 962, 1050, 2129, 2598, 4029, 4485
GlaI GCGC 6 cut(s) 438, 2370, 2741, 3834, 4110, 4218
GsaI CCCAGC 1 cut(s) 2311
GsuI CTGGAG 7 cut(s) 347, 1388, 1488, 2728, 3684, 3885, 4317
HaeII RGCGCY 1 cut(s) 440
HapII CCGG 5 cut(s) 1396, 1960, 2788, 4037, 4845
HgaI GACGC 5 cut(s) 54, 277, 1832, 2912, 4235
HhaI GCGC 6 cut(s) 439, 2371, 2742, 3835, 4111, 4219
Hin1I GRCGYC 2 cut(s) 356, 2923
Hin6I GCGC 6 cut(s) 437, 2369, 2740, 3833, 4109, 4217
HinP1I GCGC 6 cut(s) 437, 2369, 2740, 3833, 4109, 4217
HincII GTYRAC 4 cut(s) 147, 693, 1899, 4785
HindII GTYRAC 4 cut(s) 147, 693, 1899, 4785
HindIII AAGCTT 5 cut(s) 599, 1124, 3273, 3930, 4353
HpaII CCGG 5 cut(s) 1396, 1960, 2788, 4037, 4845
Hpy99I CGWCG 2 cut(s) 16, 113
HpyCH4IV ACGT 3 cut(s) 356, 3570, 4800
HpySE526I ACGT 3 cut(s) 356, 3570, 4800
Hsp92I GRCGYC 2 cut(s) 356, 2923
HspAI GCGC 6 cut(s) 437, 2369, 2740, 3833, 4109, 4217
KpnI GGTACC 1 cut(s) 4262
KroI GCCGGC 1 cut(s) 4036
KroNI GCCGGC 1 cut(s) 4038
Ksp22I TGATCA 1 cut(s) 3196
LguI GCTCTTC 1 cut(s) 427
LmnI GCTCC 9 cut(s) 765, 1469, 2463, 2747, 3727, 3805, 4051, 4388, 4683
MaeI CTAG 8 cut(s) 347, 705, 962, 1050, 2129, 2598, 4029, 4485
MaeII ACGT 3 cut(s) 356, 3570, 4800
MaeIII GTNAC 6 cut(s) 403, 774, 781, 2204, 3522, 4438
MfeI CAATTG 2 cut(s) 882, 3549
MflI RGATCY 6 cut(s) 28, 1097, 2125, 2620, 3659, 4744
MhlI GDGCHC 6 cut(s) 1285, 2223, 2460, 2998, 4059, 4423
MlsI TGGCCA 1 cut(s) 4514
MluNI TGGCCA 1 cut(s) 4514
MlyI GAGTC 7 cut(s) 1879, 1904, 1933, 3007, 3758, 4193, 4512
MmeI TCCRAC 5 cut(s) 22, 394, 2792, 4042, 4211
Mox20I TGGCCA 1 cut(s) 4514
Mph1103I ATGCAT 2 cut(s) 2812, 3982
MroNI GCCGGC 1 cut(s) 4036
MroXI GAANNNNTTC 4 cut(s) 1879, 2469, 4210, 4356
MscI TGGCCA 1 cut(s) 4514
MslI CAYNNNNRTG 7 cut(s) 134, 558, 640, 2197, 2251, 3204, 4601
Msp20I TGGCCA 1 cut(s) 4514
MspA1I CMGCKG 2 cut(s) 3041, 3476
MspI CCGG 5 cut(s) 1396, 1960, 2788, 4037, 4845
MunI CAATTG 2 cut(s) 882, 3549
Mva1269I GAATGC 2 cut(s) 220, 295
MvnI CGCG 2 cut(s) 949, 4109
NaeI GCCGGC 1 cut(s) 4038
NciI CCSGG 2 cut(s) 1397, 4845
NdeI CATATG 1 cut(s) 559
NgoMIV GCCGGC 1 cut(s) 4036
NmeAIII GCCGAG 1 cut(s) 1273
NmuCI GTSAC 2 cut(s) 403, 4438
NotI GCGGCCGC 1 cut(s) 3885
NsiI ATGCAT 2 cut(s) 2812, 3982
NspI RCATGY 4 cut(s) 41, 199, 288, 2814
NspV TTCGAA 3 cut(s) 1196, 3532, 4622
PaqCI CACCTGC 1 cut(s) 3278
PciI ACATGT 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 427
PctI GAATGC 2 cut(s) 220, 295
PdiI GCCGGC 1 cut(s) 4038
PdmI GAANNNNTTC 4 cut(s) 1879, 2469, 4210, 4356
PfeI GAWTC 7 cut(s) 535, 887, 1786, 2957, 3365, 3838, 4318
PflFI GACNNNGTC 1 cut(s) 3643
PflMI CCANNNNNTGG 2 cut(s) 1813, 2800
PfoI TCCNGGA 2 cut(s) 1507, 4249
PinAI ACCGGT 1 cut(s) 2787
PleI GAGTC 7 cut(s) 1879, 1903, 1932, 3007, 3758, 4193, 4512
PpsI GAGTC 7 cut(s) 1879, 1903, 1932, 3007, 3758, 4193, 4512
PpuMI RGGWCCY 4 cut(s) 3062, 3667, 3906, 4481
PscI ACATGT 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 1827
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 4 cut(s) 3062, 3667, 3906, 4481
PspFI CCCAGC 1 cut(s) 2307
PspPPI RGGWCCY 4 cut(s) 3062, 3667, 3906, 4481
PsrI GAACNNNNNNTAC 2 cut(s) 2034, 2066
PstI CTGCAG 3 cut(s) 3142, 3421, 3565
PstNI CAGNNNCTG 3 cut(s) 25, 1109, 4907
PsuI RGATCY 6 cut(s) 28, 1097, 2125, 2620, 3659, 4744
PsyI GACNNNGTC 1 cut(s) 3643
PvuII CAGCTG 1 cut(s) 3476
RsaI GTAC 9 cut(s) 181, 250, 670, 1535, 1654, 2043, 3155, 3467, 4260
RsaNI GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3154, 3466, 4259
RseI CAYNNNNRTG 7 cut(s) 134, 558, 640, 2197, 2251, 3204, 4601
SapI GCTCTTC 1 cut(s) 427
SchI GAGTC 7 cut(s) 1879, 1904, 1933, 3007, 3758, 4193, 4512
SduI GDGCHC 6 cut(s) 1285, 2223, 2460, 2998, 4059, 4423
SfcI CTRYAG 4 cut(s) 369, 3138, 3417, 3561
SfuI TTCGAA 3 cut(s) 1196, 3532, 4622
SmiMI CAYNNNNRTG 7 cut(s) 134, 558, 640, 2197, 2251, 3204, 4601
SmlI CTYRAG 6 cut(s) 239, 2640, 3015, 3936, 4023, 4310
SmoI CTYRAG 6 cut(s) 239, 2640, 3015, 3936, 4023, 4310
SspI AATATT 1 cut(s) 1152
SspMI CTAG 8 cut(s) 347, 705, 962, 1050, 2129, 2598, 4029, 4485
StyI CCWWGG 4 cut(s) 792, 1833, 2576, 4177
TaiI ACGT 3 cut(s) 359, 3573, 4803
TatI WGTACW 2 cut(s) 1533, 2041
TauI GCSGC 8 cut(s) 1206, 2923, 3041, 3887, 3890, 4109, 4465, 4766
TfiI GAWTC 7 cut(s) 535, 887, 1786, 2957, 3365, 3838, 4318
TseFI GTSAC 2 cut(s) 403, 4438
Tsp45I GTSAC 2 cut(s) 403, 4438
TspGWI ACGGA 2 cut(s) 2687, 3306
Tth111I GACNNNGTC 1 cut(s) 3643
Van91I CCANNNNNTGG 2 cut(s) 1813, 2800
VspI ATTAAT 1 cut(s) 834
XagI CCTNNNNNAGG 1 cut(s) 3729
XbaI TCTAGA 2 cut(s) 961, 2128
XceI RCATGY 4 cut(s) 41, 199, 288, 2814
XcmI CCANNNNNNNNNTGG 1 cut(s) 639
XmiI GTMKAC 1 cut(s) 3323
XmnI GAANNNNTTC 4 cut(s) 1879, 2469, 4210, 4356
XspI CTAG 8 cut(s) 347, 705, 962, 1050, 2129, 2598, 4029, 4485
ZraI GACGTC 1 cut(s) 357
Zsp2I ATGCAT 2 cut(s) 2812, 3982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.