MD15G1280900.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008489
Physical Location & Seq
Forward (+)
25095791 .. 25109428
13638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1280900.v1.1.491

Sequence Viewer

Length: 4815 bp
ATGGAGTGGACGACGGTGCAGCATCTGGATCTACGACATGTGGGACACAGCTCAAAGCCGTTGCAGCCTCATGCTGCTGCCTTTCATCCCCATCAGGCCCTCGTCGCCGTTGCCATTGGAAATTACATCATTGAAATGGATGCATTGACAGGAAGCAAGATTTCATCTATTGATATTGGTACCCCCGTTGTCCGCATGTCCTATAGCCCCACAAGTGGGCATTCTGTGGTAGCGATCGTTGAGGACTGTACCATTCGCTCTTGTGATTTTGATGCTGAGCAAACATGCGTCTTGCATTCACCTGAGAAGAAGACAGAACAAATTTCTTCTGATACAGAAGTCCATCTCGCTTTGACGCCTCTCCAGCCTGTAGTGTTTTTCGGTTTCCACAAGAAAATGAGTGTCACAGTTGTTGGAACTGTTGAAGGAGGAAGAGTGCCTACAAAAATAAAGACAGATTTGAAGAAACCTATTGTCAATCTCGCATGCCACCCTCGCCACCCTGTCCTGTATGTAGCTTATGCTGATGGTTTGATTCGAGCCTACAACATTCACACCTATGCTGTTCATTACACCTTACAAATTGATAACACCATAAAGCTCATTGGTGCTGGTGCATTTGGTTTTCACCCAACATTGGAGTGGATCTTTGTTGGTGATCGACGGGGTACTCTGCTCGCATGGGATGTGTCAACTGAGAGACCTAGTATGATTGGAATTACACAAGTGGGTTCCCAACCAATAGCTTCAGTTTCTTGGCTCCCATTGTTGCGGTTACTTGTGACTGTGTCCCAGGATGGAACTCTTCAAGTGTGGAAAACACGGGTTATAATTAATCCGAATAGACCTCCAATGCAAGCAAATTTCTTTGAGCCTGCTGCAATTGAATATCTTGATATCCCTCGCATCCTCTCTCAGCAGGGTGGAGAAGCAGCTTACCCTTTACCGCGAATTAAAACTCTAGAAGTTCACCCAAAATTAAATTTAGCAGCACTTCTGTTTGCAAATATGACTGGTGGAGACAATGTGAAAAATAGGGCAGCATACACTAGGGAAGGAAGGAAACAGCTGTTTGCAGTTTTGCAAGGTGCAAGAGGGTCTTCAGCATCTGTTTTGAAAGAAAAGCTTTCAGCTTTGGGTTCATCTGGAATATTAGCTGAACATCAACTTCAAGCTCAACTGCAAGAACATCATCTGAAAGGCCACAGTCAGCTGACAATATCAGACATTGCCCGGAAGGCTTTTCTCCACAGCCATTTTATGGAAGGTCATGCCAAAAGTGCCCCAATATCTCGGCTGCCCCTCATTACTATTGTAGACACTAAGCATCATCTGAAGGATGTTCCTGTATTTCAGCCTTTTCACGTGGAGTTAAATTTCTTCAATAAAGAGAATCGGGTTCTTCATTATCCTGTCAGGGCCTTTTTTGTTGATGGATTACACATTATGGCATATAACATTTGTTCTGGAGCTGACAGTATCTACAAAAAACTTTACATGACGGTTCCTGGAAATGTGGAATATCATCCGAAGTACATGGCCTACAGTAAAAAACAGGGCCTATTTCTTGTTGTATATGAGTTCAGTGGTGCTACAAATGAAGTGGTGCTTTATTTTGAAAATACCGATTCTCAGGCAGCAAACAGCAAATGTACCACTATAAAAGGTCGTGATGCAGCATTTATTGGACCCAATGAAAATCAGTTTGCAATTCTTGATGATGATAAGACTGGACTGGTTTTGTATATTCTGCCTAAAAAGGCTTCTCCTGAAGCTAATGAGAAGATTCTATTGGCTGAAGAACGCCAACCTGTGGATACTGACACTGGTCCCAAGGGTCCAATGCAATTTATGTTTGAAAGTGAAATTGATCGAATTTTTTCGACTCCAATAGAGTCAACATTGATGTTTGCTTCTCATGGGAATCAGATTGGCTTGGCAAAGTTGATTCAAGGATCCCAGCTTTCTAATTCTGATGGTCATTATATAGCAACAAAGGGTGAAGGGAAAAAGTCAATTAAGTTGAAACTAAATGAGATTGTACTTCAGGTTCACTGGCAAGAAACTCTTAGGGGCTATGTAGCTGGAATATTAACGACGCAAAGGTTTGAGCACTTGACCTCTATTCACTTTAGATCTCTTTTATGGGTTGGACCTGCACTTCTCTTTTCTACTACCACTGCAATTAGCGTTCTTGGCTGGGACGGTAGAGTAAGGACTATTCTCTCTATCAGTATGCCTTATGCAGTTTTGGTTGGTGCGCTGAATGATCGGTTGTTGCTTGCTACCCCAACAGAAATAAACCCCAGACAAAAGAAGGGAGTTGAGATTAAGAGCTGTCTTGTTGGGCTTCTTGAACCTCTTCTTATTGGATTTGCCACAATGCAAGAGAGATTTGAGCAGAAGCTTGACCTTCCAGAGATATTATATCAAATAACATCAAGGTTTGACAGCCTGCGTATTACTCCAAGGTCTCTTGATATTCTTGCTAGAGGCTCTCCTGTTTGTGGAGATCTTTCTGTGTCGCTATCTCAAGCAGGACCACAGTTCACTCAGGTGTTGAGGGGTGTCTATGCTATCAAAGCTCTCCGGTTTACAACTGCTTTGTCTGTATTAAAAGACGAATTTCTGCGCTCTAGAGATTATCCAAGATGTCCTCCAACCTCTCATTTGTTCCACCAGTTCCGCCAGTTGGGTTATGCATGTATCAAGTTTGGGCAGTTTGATAGTGCAAAAGAAACCTTTGAAGTTATAGCTGATTATGAAAGTATGCTCGACCTCTTTATATGCCACCTTAACCCCAGTGCCATGCGGCGTCTTGCTCAAAAGCTGGAAGAAGATGGCACAGATTCAGAATTAAGACGATACTGTGAAAGGATTTTAAGAGTCCGTTCTACTGGATGGACTCAAGGAATTTTCGCTAACTTTGCTGCTGAGAGTATGGTCCCTAAAGGTCCTGAATGGGGTGGTGGGAACTGGGAGATCAAAACCCCAACAAACATGAAGGCTGTTCCTCAGTGGGAACTGGCTGCAGAGGTGATGCCGTACATGAAGACTGACGATGGCACCATCCCATCCATTATCGCTGATCATATTGGTGTTTACTTGGGATCAATCAAAGGAAGAGGCAACATTGTAGAAGTAAGGGAAGATAGCTTGGTCAAAGCTTTTATATCCGCAGGTGGTGACAAGAAGCAAAATGGTCTTCCGTTGTCTAAATCCACATCTAATGTGTCCAAGGGAGTGCCTGGTGGTGGTTCGTTGATGGGTCTGGAAACACTTAACAAACAATTTGCCAGTTCCTCTGCAGCAGATGAACAGGCTAAAGCCGAAGAAGAATTCAAGAAAACAATGTACGGTGCAGCTGATGGCAGCAGCAGTGATGAAGAGGGAACTTCAAAAGCTAAAAAGTTACACATTCGAATAAGAGACAAGCCCATTGCTTCTACTGCAGTGGACGTGGATAAAATCAAAGAAGCCACGAAGCAGCTCAAACTTGGTGAAGGGTTGGGTCCACCTATGACCAGGACCAAATCATTGACTATGGGATCCCAGGACCTTAGCCAGATGTTATCCCAACCACCTCCTCCAGCTAACAGTGGGTCAATGGCTCCTCGTGTAGGCTCTGCCCCTGGTGATCTATTTGGTATGGACTCGTTTACACAACCTGCAACGGTATCACATCAAGCTCCTACCTCAACAGTTAAGGGGGTTGGAGCTGCACCTATTCCAGAGGACTTTTTCCAGAATACAATACCATCCCTTCAGGTTGCAGCCAAACTGCCCCCTCCTGGGACCTATCTTTCAAAAATGGATCAAGCTTCTCAAGGGTTTGAAAGCAACAAGGAAGCATTTAACCAGGCCAATGCATCCAGTGCCAATGTTCGTCTTCCTGATGCTGGTGTTCCCCCTCAAGCTAGTCAACTGGCTGCTCCATTCGAGCCTGTTGGACTTCCTGATGGTGGTGTCCCACCATCCTCAGGCCAGGTTGCTGCGCAGCAACAATCTCATATTCAATCAACTCAGTTCCCAGTTTCTACTCAACCTCTTGATCTTAGTGTCCTTGGGGTTCCAAATTCTACTGACTCGGGAAAACCTTCAGTGCAGCCTCCTTCTCCACCATCTTCTGTGCGTCCTGGACAGGTACCCCGTGGTGCTGCAGCTTCCATATGTTTTAAGACTGGAGTTGCCCACCTTGAGCAGAATCAACTTTCAGATGCATTGTCTTGCTTTGACGAAGCTTTTCTTGCATTGGCTAAGGATCAGTCTCGTGGAGCTGATATTAAAGCACAAGGGACCATCTGTGCGCAATACAAGATAGCAGTCACTCTTCTTCGGGAAATTGGGCGGCTGCAAAGAGTTCAAGGTCCTAGTGCAATCAGTGCAAAAGATGAGATGGCAAGACTTTCACGCCACCTGGGTTCGTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATCCGAACTGCGATTAAGCGGAACATGGAGGTGCAGAATTATGCTTATTCAAAGCAGATGCTTGAACTCCTGCTATCCAAAGCACCTCCAAGTAAGCAGGAAGAGTTGAGGAGCTTAGTTGATATGTGTGTGCAAAGGGGTCTGACCAACAAGTCCATTGATCCACTTGAAGATCCTTCCCAGTTCTGTGCCGCCACACTTAGTCGTCTGTCAACTATTGGATATGACGTTTGTGATCTTTGTGGGGCCAAATTTTCAGCTCTCTCAGCACCCGGTTGCATTATATGCGGTATGGGAAGCATTAAAAGATCAGATGCTCGCACAGGGCCAGGGCCTGTTCCTTCACCGTTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1605

Amino Acids

174.86

Weight (kDa)

8.6

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 17 - 272 5.8e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 152 - 274 4.8e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 155 - 275 8.2e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 155 - 277 9.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 161 - 274 6.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AarI CACCTGC 1 cut(s) 3170
AasI GACNNNNNNGTC 1 cut(s) 4609
Acc16I TGCGCA 2 cut(s) 3996, 4308
Acc36I ACCTGC 3 cut(s) 2164, 3170, 3676
Acc65I GGTACC 2 cut(s) 179, 4144
AccB1I GGYRCC 3 cut(s) 179, 3065, 4144
AccB7I CCANNNNNTGG 2 cut(s) 1261, 1813
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 1 cut(s) 949
AcuI CTGAAG 8 cut(s) 732, 1086, 1355, 1791, 1818, 2030, 3749, 4083
AcvI CACGTG 1 cut(s) 1366
AcyI GRCGYC 2 cut(s) 356, 2815
AfaI GTAC 9 cut(s) 181, 250, 670, 1535, 1654, 2043, 3047, 3356, 4146
AflIII ACRYGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 3460
AjuI GAANNNNNNNTTGG 2 cut(s) 1775, 1807
AleI CACNNNNGTG 1 cut(s) 2555
Alw21I GWGCWC 1 cut(s) 2115
Alw26I GTCTC 5 cut(s) 694, 1014, 2478, 3423, 4272
AlwNI CAGNNNCTG 3 cut(s) 25, 1109, 4793
Ama87I CYCGRG 1 cut(s) 4087
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 4 cut(s) 1879, 2361, 4096, 4242
Asp718I GGTACC 2 cut(s) 179, 4144
AspLEI GCGC 4 cut(s) 2263, 2634, 3997, 4309
AsuC2I CCSGG 2 cut(s) 1234, 4731
AsuII TTCGAA 1 cut(s) 3421
AvaI CYCGRG 1 cut(s) 4087
AxyI CCTNAGG 1 cut(s) 3979
BaeGI GKGCMC 1 cut(s) 1285
BaeI ACNNNNGTAYC 4 cut(s) 171, 204, 324, 357
BamHI GGATCC 2 cut(s) 1955, 3548
BanI GGYRCC 3 cut(s) 179, 3065, 4144
BarI GAAGNNNNNNTAC 2 cut(s) 424, 456
BauI CACGAG 2 cut(s) 3615, 4269
BbrPI CACGTG 1 cut(s) 1366
BbsI GAAGAC 5 cut(s) 317, 1092, 3059, 3197, 3881
Bbv12I GWGCWC 1 cut(s) 2115
BceAI ACGGC 3 cut(s) 43, 92, 3028
BciVI GTATCC 2 cut(s) 1810, 4469
BclI TGATCA 1 cut(s) 3088
BcnI CCSGG 2 cut(s) 1234, 4731
BcoDI GTCTC 5 cut(s) 694, 1014, 2478, 3423, 4272
BfaI CTAG 7 cut(s) 705, 962, 1050, 2490, 2637, 3918, 4371
BfmI CTRYAG 7 cut(s) 202, 369, 1543, 3030, 3306, 3450, 4158
BfuAI ACCTGC 3 cut(s) 2164, 3170, 3676
BfuI GTATCC 2 cut(s) 1810, 4469
BglI GCCNNNNNGGC 1 cut(s) 1238
BglII AGATCT 2 cut(s) 2135, 2512
BlpI GCTNAGC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 4087
BmgBI CACGTC 1 cut(s) 3460
BmrI ACTGGG 4 cut(s) 2796, 2986, 4025, 4633
BmuI ACTGGG 4 cut(s) 2796, 2986, 4025, 4633
BoxI GACNNNNGTC 1 cut(s) 1827
BpiI GAAGAC 5 cut(s) 317, 1092, 3059, 3197, 3881
BpmI CTGGAG 4 cut(s) 347, 1488, 3573, 4203
Bpu10I CCTNAGC 2 cut(s) 3560, 4257
Bpu1102I GCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 3421
BpuEI CTTGAG 5 cut(s) 2517, 2892, 3810, 3897, 4217
BpuMI CCSGG 2 cut(s) 1234, 4731
BsaAI YACGTR 1 cut(s) 1366
BsaHI GRCGYC 2 cut(s) 356, 2815
BsaI GGTCTC 2 cut(s) 694, 2478
BsaWI WCCGGW 1 cut(s) 2589
BsaXI ACNNNNNCTCC 4 cut(s) 420, 450, 1011, 1041
Bse21I CCTNAGG 1 cut(s) 3979
Bse3DI GCAATG 2 cut(s) 1227, 3438
BseMI GCAATG 2 cut(s) 1227, 3438
BseRI GAGGAG 3 cut(s) 3576, 3603, 4582
BseSI GKGCMC 1 cut(s) 1285
BseYI CCCAGC 2 cut(s) 1959, 2199
BsgI GTGCAG 6 cut(s) 38, 2142, 3381, 3705, 4124, 4511
Bsh1236I CGCG 1 cut(s) 949
Bsh1285I CGRYCG 1 cut(s) 237
BshNI GGYRCC 3 cut(s) 179, 3065, 4144
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 2115
BsiHKCI CYCGRG 1 cut(s) 4087
BsiSI CCGG 3 cut(s) 1234, 2590, 4731
BslFI GGGAC 8 cut(s) 57, 775, 1815, 2216, 2930, 3808, 3953, 4309
BsmAI GTCTC 5 cut(s) 694, 1014, 2478, 3423, 4272
BsmFI GGGAC 8 cut(s) 57, 775, 1815, 2216, 2930, 3808, 3953, 4309
BsmI GAATGC 2 cut(s) 220, 295
Bso31I GGTCTC 2 cut(s) 694, 2478
BsoBI CYCGRG 1 cut(s) 4087
Bsp119I TTCGAA 1 cut(s) 3421
Bsp1286I GDGCHC 2 cut(s) 1285, 2115
Bsp1720I GCTNAGC 1 cut(s) 276
BspFNI CGCG 1 cut(s) 949
BspMAI CTGCAG 4 cut(s) 3034, 3310, 3454, 4162
BspMI ACCTGC 3 cut(s) 2164, 3170, 3676
BspT104I TTCGAA 1 cut(s) 3421
BspT107I GGYRCC 3 cut(s) 179, 3065, 4144
BspTNI GGTCTC 2 cut(s) 694, 2478
BsrDI GCAATG 2 cut(s) 1227, 3438
BssNI GRCGYC 2 cut(s) 356, 2815
BssSI CACGAG 2 cut(s) 3615, 4269
BssT1I CCWWGG 4 cut(s) 1833, 2468, 3237, 4063
Bst2BI CACGAG 2 cut(s) 3615, 4269
Bst6I CTCTTC 8 cut(s) 427, 810, 2367, 3118, 3381, 4335, 4437, 4554
BstACI GRCGYC 2 cut(s) 356, 2815
BstAPI GCANNNNNTGC 3 cut(s) 3875, 4382, 4743
BstBAI YACGTR 1 cut(s) 1366
BstBI TTCGAA 1 cut(s) 3421
BstC8I GCNNGC 7 cut(s) 487, 678, 858, 876, 2283, 2456, 4777
BstDSI CCRYGG 1 cut(s) 4150
BstENI CCTNNNNNAGG 1 cut(s) 3618
BstFNI CGCG 1 cut(s) 949
BstHHI GCGC 4 cut(s) 2263, 2634, 3997, 4309
BstMAI GTCTC 5 cut(s) 694, 1014, 2478, 3423, 4272
BstMCI CGRYCG 1 cut(s) 237
BstNSI RCATGY 5 cut(s) 41, 199, 288, 489, 2706
BstPAI GACNNNNGTC 1 cut(s) 1827
BstSFI CTRYAG 7 cut(s) 202, 369, 1543, 3030, 3306, 3450, 4158
BstSLI GKGCMC 1 cut(s) 1285
BstUI CGCG 1 cut(s) 949
BstV2I GAAGAC 5 cut(s) 317, 1092, 3059, 3197, 3881
BstX2I RGATCY 7 cut(s) 28, 645, 1955, 2135, 2512, 3548, 4630
BstYI RGATCY 7 cut(s) 28, 645, 1955, 2135, 2512, 3548, 4630
Bsu36I CCTNAGG 1 cut(s) 3979
BsuI GTATCC 2 cut(s) 1810, 4469
BtgI CCRYGG 1 cut(s) 4150
BtrI CACGTC 1 cut(s) 3460
BtsI GCAGTG 3 cut(s) 2178, 3385, 3459
BveI ACCTGC 3 cut(s) 2164, 3170, 3676
Cac8I GCNNGC 7 cut(s) 487, 678, 858, 876, 2283, 2456, 4777
CaiI CAGNNNCTG 3 cut(s) 25, 1109, 4793
CfoI GCGC 4 cut(s) 2263, 2634, 3997, 4309
CseI GACGC 5 cut(s) 277, 364, 2107, 2804, 4121
Csp6I GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3046, 3355, 4145
CspCI CAANNNNNGTGG 2 cut(s) 1584, 1619
CviQI GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3046, 3355, 4145
DrdI GACNNNNNNGTC 1 cut(s) 4609
DseDI GACNNNNNNGTC 1 cut(s) 4609
Eam1104I CTCTTC 8 cut(s) 427, 810, 2367, 3118, 3381, 4335, 4437, 4554
EarI CTCTTC 8 cut(s) 427, 810, 2367, 3118, 3381, 4335, 4437, 4554
EciI GGCGGA 1 cut(s) 2675
Eco130I CCWWGG 4 cut(s) 1833, 2468, 3237, 4063
Eco31I GGTCTC 2 cut(s) 694, 2478
Eco32I GATATC 1 cut(s) 898
Eco57I CTGAAG 8 cut(s) 732, 1086, 1355, 1791, 1818, 2030, 3749, 4083
Eco72I CACGTG 1 cut(s) 1366
Eco81I CCTNAGG 1 cut(s) 3979
Eco88I CYCGRG 1 cut(s) 4087
EcoNI CCTNNNNNAGG 1 cut(s) 3618
EcoO109I RGGNCCY 8 cut(s) 97, 1419, 1558, 2954, 3556, 3795, 4367, 4790
EcoRI GAATTC 1 cut(s) 3338
EcoRV GATATC 1 cut(s) 898
EcoT14I CCWWGG 4 cut(s) 1833, 2468, 3237, 4063
EcoT22I ATGCAT 4 cut(s) 145, 2704, 3871, 4222
ErhI CCWWGG 4 cut(s) 1833, 2468, 3237, 4063
FaqI GGGAC 8 cut(s) 57, 775, 1815, 2216, 2930, 3808, 3953, 4309
FauNDI CATATG 1 cut(s) 4169
FbaI TGATCA 1 cut(s) 3088
FblI GTMKAC 1 cut(s) 1317
FspBI CTAG 7 cut(s) 705, 962, 1050, 2490, 2637, 3918, 4371
FspI TGCGCA 2 cut(s) 3996, 4308
GlaI GCGC 4 cut(s) 2262, 2633, 3996, 4308
GsaI CCCAGC 2 cut(s) 1963, 2203
GsuI CTGGAG 4 cut(s) 347, 1488, 3573, 4203
HapII CCGG 3 cut(s) 1234, 2590, 4731
HgaI GACGC 5 cut(s) 277, 364, 2107, 2804, 4121
HhaI GCGC 4 cut(s) 2263, 2634, 3997, 4309
Hin1I GRCGYC 2 cut(s) 356, 2815
Hin6I GCGC 4 cut(s) 2261, 2632, 3995, 4307
HinP1I GCGC 4 cut(s) 2261, 2632, 3995, 4307
HincII GTYRAC 4 cut(s) 693, 1899, 3923, 4671
HindII GTYRAC 4 cut(s) 693, 1899, 3923, 4671
HindIII AAGCTT 5 cut(s) 1124, 2405, 3165, 3819, 4239
HpaII CCGG 3 cut(s) 1234, 2590, 4731
Hpy99I CGWCG 4 cut(s) 16, 107, 666, 2101
HpyCH4IV ACGT 3 cut(s) 1365, 3459, 4686
HpySE526I ACGT 3 cut(s) 1365, 3459, 4686
Hsp92I GRCGYC 2 cut(s) 356, 2815
HspAI GCGC 4 cut(s) 2261, 2632, 3995, 4307
KpnI GGTACC 2 cut(s) 183, 4148
Ksp22I TGATCA 1 cut(s) 3088
LmnI GCTCC 8 cut(s) 765, 1469, 3616, 3694, 3716, 3937, 4274, 4569
MaeI CTAG 7 cut(s) 705, 962, 1050, 2490, 2637, 3918, 4371
MaeII ACGT 3 cut(s) 1365, 3459, 4686
MaeIII GTNAC 6 cut(s) 403, 774, 781, 3185, 3411, 4324
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 7 cut(s) 28, 645, 1955, 2135, 2512, 3548, 4630
MhlI GDGCHC 2 cut(s) 1285, 2115
MlyI GAGTC 6 cut(s) 1879, 1904, 2895, 2899, 3647, 4079
MmeI TCCRAC 5 cut(s) 394, 2131, 2684, 3694, 3928
Mph1103I ATGCAT 4 cut(s) 145, 2704, 3871, 4222
MroXI GAANNNNTTC 4 cut(s) 1879, 2361, 4096, 4242
MslI CAYNNNNRTG 6 cut(s) 134, 558, 640, 2555, 3096, 4487
MspA1I CMGCKG 3 cut(s) 1069, 1213, 3365
MspI CCGG 3 cut(s) 1234, 2590, 4731
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 2 cut(s) 220, 295
MvnI CGCG 1 cut(s) 949
NciI CCSGG 2 cut(s) 1234, 4731
NdeI CATATG 1 cut(s) 4169
NmeAIII GCCGAG 1 cut(s) 1273
NmuCI GTSAC 4 cut(s) 403, 781, 3185, 4324
NsbI TGCGCA 2 cut(s) 3996, 4308
NsiI ATGCAT 4 cut(s) 145, 2704, 3871, 4222
NspI RCATGY 5 cut(s) 41, 199, 288, 489, 2706
NspV TTCGAA 1 cut(s) 3421
OliI CACNNNNGTG 1 cut(s) 2555
PaeI GCATGC 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 3170
PciI ACATGT 1 cut(s) 37
PctI GAATGC 2 cut(s) 220, 295
PdmI GAANNNNTTC 4 cut(s) 1879, 2361, 4096, 4242
PfeI GAWTC 8 cut(s) 535, 1393, 1628, 1786, 1924, 1948, 2849, 4204
PflFI GACNNNGTC 1 cut(s) 787
PflMI CCANNNNNTGG 2 cut(s) 1261, 1813
PfoI TCCNGGA 2 cut(s) 1507, 4135
Ple19I CGATCG 1 cut(s) 237
PleI GAGTC 6 cut(s) 1879, 1903, 2894, 2899, 3647, 4079
PmaCI CACGTG 1 cut(s) 1366
PmlI CACGTG 1 cut(s) 1366
PpsI GAGTC 6 cut(s) 1879, 1903, 2894, 2899, 3647, 4079
Ppu21I YACGTR 1 cut(s) 1366
PpuMI RGGWCCY 4 cut(s) 2954, 3556, 3795, 4367
PscI ACATGT 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 1827
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 4 cut(s) 2954, 3556, 3795, 4367
PspCI CACGTG 1 cut(s) 1366
PspFI CCCAGC 2 cut(s) 1959, 2199
PspPPI RGGWCCY 4 cut(s) 2954, 3556, 3795, 4367
PsrI GAACNNNNNNTAC 2 cut(s) 2034, 2066
PstI CTGCAG 4 cut(s) 3034, 3310, 3454, 4162
PstNI CAGNNNCTG 3 cut(s) 25, 1109, 4793
PsuI RGATCY 7 cut(s) 28, 645, 1955, 2135, 2512, 3548, 4630
PsyI GACNNNGTC 1 cut(s) 787
PvuI CGATCG 1 cut(s) 237
PvuII CAGCTG 3 cut(s) 1069, 1213, 3365
RsaI GTAC 9 cut(s) 181, 250, 670, 1535, 1654, 2043, 3047, 3356, 4146
RsaNI GTAC 9 cut(s) 180, 249, 669, 1534, 1653, 2042, 3046, 3355, 4145
RseI CAYNNNNRTG 6 cut(s) 134, 558, 640, 2555, 3096, 4487
SchI GAGTC 6 cut(s) 1879, 1904, 2895, 2899, 3647, 4079
SduI GDGCHC 2 cut(s) 1285, 2115
SfcI CTRYAG 7 cut(s) 202, 369, 1543, 3030, 3306, 3450, 4158
SfuI TTCGAA 1 cut(s) 3421
SmiMI CAYNNNNRTG 6 cut(s) 134, 558, 640, 2555, 3096, 4487
SmlI CTYRAG 5 cut(s) 2532, 2907, 3825, 3912, 4196
SmoI CTYRAG 5 cut(s) 2532, 2907, 3825, 3912, 4196
SphI GCATGC 1 cut(s) 489
SspI AATATT 2 cut(s) 1152, 2091
SspMI CTAG 7 cut(s) 705, 962, 1050, 2490, 2637, 3918, 4371
StyI CCWWGG 4 cut(s) 1833, 2468, 3237, 4063
TaiI ACGT 3 cut(s) 1368, 3462, 4689
TaqI TCGA 7 cut(s) 538, 661, 1873, 1883, 2775, 3421, 3939
TatI WGTACW 2 cut(s) 1533, 2041
TauI GCSGC 3 cut(s) 2815, 4351, 4652
TfiI GAWTC 8 cut(s) 535, 1393, 1628, 1786, 1924, 1948, 2849, 4204
TseFI GTSAC 4 cut(s) 403, 781, 3185, 4324
Tsp45I GTSAC 4 cut(s) 403, 781, 3185, 4324
TspGWI ACGGA 2 cut(s) 2879, 3198
Tth111I GACNNNGTC 1 cut(s) 787
Van91I CCANNNNNTGG 2 cut(s) 1261, 1813
VspI ATTAAT 1 cut(s) 834
XagI CCTNNNNNAGG 1 cut(s) 3618
XbaI TCTAGA 2 cut(s) 961, 2636
XceI RCATGY 5 cut(s) 41, 199, 288, 489, 2706
XcmI CCANNNNNNNNNTGG 1 cut(s) 639
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 4 cut(s) 1879, 2361, 4096, 4242
XspI CTAG 7 cut(s) 705, 962, 1050, 2490, 2637, 3918, 4371
Zsp2I ATGCAT 4 cut(s) 145, 2704, 3871, 4222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.