RchiOBHm_Chr2g0105001

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008489
Physical Location & Seq
Forward (+)
16262132 .. 16274525
12394 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47924

Sequence Viewer

Length: 4878 bp
ATGGAGTGGACGACGGTGCAGCATCTCGATCTACGACACGTCGCGCGGGGCACCAAACCCCTCCAGCCTCATGCCGCCGCCTTTCATCCGCATCAGGCCCTCGTCGCCGTCGCCATCGGCAACTACATCGTCGAAATGGATGCGTTGACGGGAAGCAAGATTGCATCTATTGATATCGGTATACCTGTTATCCGCATGGCATATAGTCCCACGAGTGGCCATTCTGTCATAGCGATCCATGAGGATGGTACTATTCGATCCTGTGATTTTGATGCTGAACAAACTTGCGTGTTGCATTCACCTGAGAAGAAGTTGGATCAAATTACTCCTGATACAGAAGTCCATCTTGCTTTGACACCTCTCCAACCTGTAGTGTTTTTCGGGTTCCATAAAAGGATGAGTGTTACAGTTGTTGGGACTGTTGAAGGAGGAAGGGCACCAACAAAAATAAAGACAGATTTGAAAAAACCTATTGTGAATCTTGCATGCCACCCTCGCCATCCCGTCCTGTACGTTGCTTATGCAGATGGTTTGATTCGAGCATACAACATTCACACCTATGCAGTTCATTACACCTTACAGATCGACAACACCATAAAGCTTATCGGTGCTGGAGCGTTTGGTTTTCACCCAACATTGGAGTGGATTTTTGTTGGTGATAGACGGGGTACACTTCTTGCATGGGATGTTTCTACTGAGAGACCTAGTATGATTGGAATTACACAAGTGGGTTCCCAACCAATTTCTTCAGTTTCTTGGCTACCAATGTTGCGGTTACTTGTAACTGTAACCAGGGATGGAACTCTTCAAGTGTGGAAGACACGTGTTATAATTAATCCAAATAGACCTCCAATGCAAGCGAATTTCTTTGAACCAGCTGCAATTGAATCACTTGACATCCCTCGCATACTTTCTCAACAGGGTGGAGAAGCAGCTTACCCTTTACCGCGTATCAAAACATTAGAAGTTCACTCCAAACTGAATTTGGCAGCGCTTCTGTTCATAAATATGACAGGTGGAGACAATGTGAAAAATAGGGCCGCATACACTAGGGAAGGAAGGAAACAACTGTTTGCAGTTTTGCAAGGGGCAAGAGGATCCTCAGCGTCTGTTTTGAAAGAAAAGCTTTCATCTTTGGGGTCGTCTGGAATATTAGCTGAACATCAACTTCAAGCTCAACTACAAGAACATCATATGAAAGGGCACAGTCATCTTACAATATCAGACATTGCTCGGAAGGCTTTTCTCCACAGTCATTTCATGGAAGGTCATGCCAAAAGTGCCCCAATATCTCGGCTGCCTCTAATTACTATTGTAGATTCTAAGCATCATCTGAAGGATGCTCCTGTATGTCAGCCATTTCACCTGGAGCTAAATTTCTTCAGTAAAGAGAATCGGGTTCTTCATTATCCTGTCAGAGCCTTTTGTATAGATGGGTCAAACCTTATGGCATATAATCTGTCTTCTGGAGCCGATAGTATCTACAAGAGACTTCATACATCGGTTCCTGCAAATGTGGAATACCATCCAAAGTACCTGTTCTACAGTAAAAGACGGCGCATATTTCTTGTTGTCTATGAATTTAGTGGTGCTACAAATGAAGTTGTGCTTTATTTTGAAAATACTGATTCTCAGGCAGCAAACAGTAAATGCAGCACAGTAAAAGGTCGAGATGCAGCATTTATTGGTCCCGATGAGAATCAGTTTGCAATTCTTGATGATGATAAGACTGGACTGGCTTTACATATACTGCCTGGAAAGGCTACTCCAGAAGCTAATGAAAAAAATCTACTTGCTGATGAAAACCAATCTATGAATACTGATACTGCTGCCCCCCGAGGTCCAATGCAATTTATGTTTGAAAGTGAAGTGGATCGAATCTTTTCCACTCCAATAGAGTCAACTCTGATGTTTGCTTCTCATGGGGATCAGATTGGCTTGGCAAAGCTGGTTCAAGGATACCGCCTTTCTAATGCTGGTGGTCATTATATAGCAACAACAAATGAGGGGAGAAAGTCAATCAAATTGAAACTTGATGAAATTGTACTTCAGGTTCACTGGCAAGAAACTCTTAGGGGCTATGTTGCAGGAATATTAACTACACAAAGAGTTCTTATAGTTTCAGCAGATCTAGATATACTGGCAGGAAGTTCTGCAAAATTTGATAAAGGACTTCCTTCAATATCCTTATTCAACATCTTGGTATCACTTTTATGGGTTGGGCCTGCACTTCTCTTTTCTACTACCACTGCAGTTAGTGTGCTTGGTTGGGATGGGAAAGTGAGGACTATACTCTCTCTCAGTATGCCTTATGCAGTTTTGGTTGGTGCGCTGAATGACCGGTTGTTGCTTGCTACCCCAACAGAAATAAATCCCAGACAAAAGAAGGGAGTTGAGATTAAGAGTTGTCTTGTTGGGCTCCTTGAACCTCTTCTTATTGGATTTGCCACAATGCAAGAAAGATTTGAGCAGAAGCTTGACCTGCCAGAGATATTGTATCAAATAACATCAAGGTTTGACAGCCTGCGTATCACTCCAAGGTCTCTTGATATTCTGGCCAGAGGCTCTCCTGTTTGTGGAGATCTTTCTGTCTCATTATCTCAAGCAGGACCACAGTTCACACAGGTATTGCGTGGTGTCTATGCTATCAAAGCTCTCCGCTTTTCTACTGCTTTATCTGTTTTAAAAGATGAATTTCTGCGCTCTAGAGATTATCCAAGGTGTCCTCCAACCTCTCATCTGTTCCACCAGTTCCGGCAGTTGGGCTATGCATGTATCAAGTTTGGTCAGTTTGATAGTGCAAAAGAGACCTTTGAAGTCATAGCAGATTATGAAAGTATGCTCGATCTGTTTATATGCCACCTTAACCCCAGTGCCATGCGGCGTCTTGCTCAAAAATTGGAAGAAGATGGCACTGATTCAGAACTGAGGAGATACTGTGAGAGGATCTTAAGAGTGCGCTCTACTGGATGGACTCAAGGCATTTTTGCTAACTTTGCTGCTGAGAGTATGGTTCCGAAGGGTCCTGAATGGGGTGGTGGGAACTGGGAAATCAAAACCCCAACTAATATGAAGGCAATACCTCAATGGGAACTGGCTGCCGAGGTGATGCCATACATGAGGACTGATGACGGTGCCATTCCATCCATTATTGCTGATCATATTGGTGTTTACTTGGGTTCCATCAGAGGACGAGGCAACATTGTAGAAGTAAGGGAAGATAGCTTGGTCAAAGCTTTCAAACCCGCAGGTGGTGATAATAAGCCAAATGGTCTTCAAGACTCTTCAGTGACATCTACATCTAATATGTCCAAGGGAGTGCCTGGTGGTGGTTCATTGATGGGTCTTGAAACACTCACCAAACAAGTAGCCAGTTCCACCGCGGCAGATGAACAGGCTAAAGCTGAAGAAGAATTTAAGAAATCTATGTATGGTGCAGCTGATGGTAGCAGCAGTGATGAGGAGGGAACTTCAAAAACTAAAAGGTTACAGATTCGGATAAGAGACAAGCCAATTGCGTCTACCACAGTAGATGTTGATAAGATCAAAGAAGCCACGAAGCAATTCAAACTTGGTGAGGGGTTGGCTCGCCCCAGCAGAACCAAGTCATTGACTGGATCCCAGGACTTAAGCCAGATGTTATCCCAACCGCCTCCAGCCAGTAGTGGTTTTCCAAATGCTCGTGTAGGTTCTGCCCCTGGTGATCTGTTTGGTATGGATGCACCAACACAACCTGCAACTGTATCACAGCAAGCTCCTGTACCAGGTGTGGGAATGACAGCTGGACCAATTCCAGAGGACTTTTTCCAAAATACCATACCGTCCTTCCAGGTTGCAGCCTCACTGCCCCCTCCTGGAACCTATCTTTCAAAGATGGATCAAGCCTCTCAGGGGGCTGAAAGAAATACGGAAACACTTAACCAGGTCAATGCACCCAAAGCCCATATAGATCTTCCTGATGGTGGTGTTCCCCCTCAAGCTACTCAACAGGGTGTTCCACTGGGGTCCTTTGGACTTCCTGATGGTGGGGTTCCACCACAAGCCCCAAGCCAGGCTACTGTTCAACAGACTCAGGTTCAGTTAGCTCAACCTCCAATATCCACTCAGCCTCTTGATCTAAGTGCCCTTGGCATTCCGAATTCTGCCGATAATGGAAAACCTACTGGGCAGCCTCCTTCACCACCGTCTGCTGTGCGTCCTGGACAGGTTCCCCGTGGAGCAGCCGCTCCTATATGCTTTAAGACTGGCGTATCCCACCTTGAGCAGAATCAACTTTCGGACGCATTATCCTGCTTCGATGAAGCTTTTCTTGCACTAGCGAAGGACCACTCTCGCGGAGCTGATATCAAAGCACAAGCGACCATCTGTGCTCAATATAAGATAGCAGTCACTCTTCTCCAGGAAATTGGCCGGCTTCAGAGAGTCCATGGCCCTAGTGCAATTAGTGCAAAAGATGAGATGGCAAGACTAGCACGCCACCTGGGTTCTTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATTCGAACTGCCATTAAGAGGAATATGGAAGTGCAAAATTATGCTTATTCAAAACAGATGCTTGAACTCCTGCTGTCCAAAGCACCTCCAAGTAAGCAGGATGAGCTAAGGAGCTTGGTTGACATGTGTGTTCAAAGGGGTCTGTCCAACAAGTCCATTGATCCTCTTGAAGACCCTTCCCAGTTCTGTGCGGCCACACTTAGCCGTCTGTCAACCATTGGATACGATGTTTGTGACCTTTGTGGGGCTAAATTTTCGGCTCTCGCAACGCCTGGTTGCATTATATGCGGTATGGGAAGCATCAAAAGATCAGATGCACTTACAGGGCCGGGGCCTGTTCCTTCGCCGTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1625

Amino Acids

176.66

Weight (kDa)

8.32

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 72 - 272 3.4e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 155 - 275 3.9e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 155 - 277 1.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 161 - 274 5.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AarI CACCTGC 1 cut(s) 3239
Acc36I ACCTGC 3 cut(s) 2490, 3239, 3742
AccB1I GGYRCC 3 cut(s) 50, 436, 3134
AccBSI CCGCTC 1 cut(s) 4226
AccI GTMKAC 2 cut(s) 181, 3521
AccII CGCG 5 cut(s) 44, 46, 949, 3383, 4335
AcoI YGGCCR 4 cut(s) 217, 2556, 4408, 4713
AcsI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2693, 3413, 4138, 4772
AcuI CTGAAG 7 cut(s) 732, 1355, 1366, 2033, 3270, 3426, 4400
AcvI CACGTG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 2884
AfaI GTAC 6 cut(s) 250, 512, 670, 1535, 2046, 3762
AfeI AGCGCT 1 cut(s) 993
AflII CTTAAG 2 cut(s) 2950, 3628
AflIII ACRYGT 4 cut(s) 37, 821, 823, 4644
AgeI ACCGGT 1 cut(s) 2340
AjiI CACGTC 1 cut(s) 40
Alw21I GWGCWC 1 cut(s) 4372
Alw26I GTCTC 7 cut(s) 694, 1014, 1483, 2547, 2596, 2801, 3498
AlwNI CAGNNNCTG 1 cut(s) 1109
Ama87I CYCGRG 1 cut(s) 1836
Aor51HI AGCGCT 1 cut(s) 993
ApoI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2693, 3413, 4138, 4772
AseI ATTAAT 1 cut(s) 834
AsiGI ACCGGT 1 cut(s) 2340
Asp700I GAANNNNTTC 4 cut(s) 1882, 2430, 3563, 4305
AspLEI GCGC 6 cut(s) 46, 994, 1560, 2332, 2703, 2961
AsuC2I CCSGG 1 cut(s) 4851
AsuII TTCGAA 1 cut(s) 4525
AvaI CYCGRG 1 cut(s) 1836
AvaII GGWCC 7 cut(s) 1688, 1841, 2609, 3023, 3785, 4005, 4324
BaeGI GKGCMC 5 cut(s) 53, 439, 1206, 1285, 4126
BaeI ACNNNNGTAYC 4 cut(s) 324, 357, 4233, 4266
BalI TGGCCA 2 cut(s) 219, 2558
BamHI GGATCC 2 cut(s) 1097, 3617
BanI GGYRCC 3 cut(s) 50, 436, 3134
BanII GRGCYC 1 cut(s) 2421
BauI CACGAG 2 cut(s) 211, 3681
BbrPI CACGTG 1 cut(s) 824
BbsI GAAGAC 4 cut(s) 824, 1455, 3266, 4698
Bbv12I GWGCWC 1 cut(s) 4372
BbvCI CCTCAGC 1 cut(s) 1102
BceAI ACGGC 4 cut(s) 92, 1571, 4710, 4852
BcgI CGANNNNNNTGC 4 cut(s) 109, 122, 143, 156
BciVI GTATCC 3 cut(s) 1952, 4261, 4736
BclI TGATCA 1 cut(s) 3157
BcnI CCSGG 1 cut(s) 4851
BcoDI GTCTC 7 cut(s) 694, 1014, 1483, 2547, 2596, 2801, 3498
BfaI CTAG 7 cut(s) 705, 1050, 2132, 2706, 4316, 4434, 4469
BfmI CTRYAG 3 cut(s) 369, 1543, 2250
BfoI RGCGCY 1 cut(s) 995
BfrI CTTAAG 2 cut(s) 2950, 3628
BfuAI ACCTGC 3 cut(s) 2490, 3239, 3742
BfuI GTATCC 3 cut(s) 1952, 4261, 4736
BglII AGATCT 3 cut(s) 2128, 2581, 3949
Bme18I GGWCC 7 cut(s) 1688, 1841, 2609, 3023, 3785, 4005, 4324
BmeT110I CYCGRG 1 cut(s) 1836
BmgBI CACGTC 1 cut(s) 40
BmrI ACTGGG 5 cut(s) 2865, 3055, 4010, 4173, 4696
BmuI ACTGGG 5 cut(s) 2865, 3055, 4010, 4173, 4696
BpiI GAAGAC 4 cut(s) 824, 1455, 3266, 4698
BpmI CTGGAG 7 cut(s) 47, 633, 1388, 1488, 1752, 3639, 4382
Bpu10I CCTNAGC 2 cut(s) 1102, 4628
Bpu14I TTCGAA 1 cut(s) 4525
BpuEI CTTGAG 4 cut(s) 2586, 2961, 3960, 4280
BpuMI CCSGG 1 cut(s) 4851
BsaAI YACGTR 1 cut(s) 824
BsaBI GATNNNNATC 1 cut(s) 1698
BsaHI GRCGYC 1 cut(s) 2884
BsaI GGTCTC 3 cut(s) 694, 2547, 2801
BsaWI WCCGGW 1 cut(s) 2340
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 2923, 2953
Bse118I RCCGGY 2 cut(s) 2340, 4410
Bse3DI GCAATG 1 cut(s) 1227
Bse8I GATNNNNATC 1 cut(s) 1698
BseJI GATNNNNATC 1 cut(s) 1698
BseMI GCAATG 1 cut(s) 1227
BseRI GAGGAG 2 cut(s) 2944, 3476
BseSI GKGCMC 5 cut(s) 53, 439, 1206, 1285, 4126
BseYI CCCAGC 1 cut(s) 3593
BsgI GTGCAG 3 cut(s) 38, 2211, 3456
Bsh1236I CGCG 5 cut(s) 44, 46, 949, 3383, 4335
BshNI GGYRCC 3 cut(s) 50, 436, 3134
BshTI ACCGGT 1 cut(s) 2340
BsiHKAI GWGCWC 1 cut(s) 4372
BsiHKCI CYCGRG 1 cut(s) 1836
BsiSI CCGG 4 cut(s) 2341, 2755, 4411, 4850
BslFI GGGAC 3 cut(s) 192, 430, 1674
BsmAI GTCTC 7 cut(s) 694, 1014, 1483, 2547, 2596, 2801, 3498
BsmFI GGGAC 3 cut(s) 192, 430, 1674
BsmI GAATGC 2 cut(s) 295, 4131
Bso31I GGTCTC 3 cut(s) 694, 2547, 2801
BsoBI CYCGRG 1 cut(s) 1836
Bsp119I TTCGAA 1 cut(s) 4525
Bsp1286I GDGCHC 7 cut(s) 53, 439, 1206, 1285, 2421, 4126, 4372
Bsp19I CCATGG 1 cut(s) 4426
BspFNI CGCG 5 cut(s) 44, 46, 949, 3383, 4335
BspMAI CTGCAG 1 cut(s) 2254
BspMI ACCTGC 3 cut(s) 2490, 3239, 3742
BspT104I TTCGAA 1 cut(s) 4525
BspT107I GGYRCC 3 cut(s) 50, 436, 3134
BspTI CTTAAG 2 cut(s) 2950, 3628
BspTNI GGTCTC 3 cut(s) 694, 2547, 2801
BsrBI CCGCTC 1 cut(s) 4226
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 2 cut(s) 2340, 4410
BssAI RCCGGY 2 cut(s) 2340, 4410
BssNAI GTATAC 1 cut(s) 182
BssNI GRCGYC 1 cut(s) 2884
BssSI CACGAG 2 cut(s) 211, 3681
BssT1I CCWWGG 5 cut(s) 2537, 2717, 3312, 4126, 4426
Bst1107I GTATAC 1 cut(s) 182
Bst2BI CACGAG 2 cut(s) 211, 3681
Bst6I CTCTTC 5 cut(s) 810, 2436, 3289, 4398, 4500
BstACI GRCGYC 1 cut(s) 2884
BstAFI CTTAAG 2 cut(s) 2950, 3628
BstAPI GCANNNNNTGC 2 cut(s) 4445, 4806
BstBAI YACGTR 1 cut(s) 824
BstBI TTCGAA 1 cut(s) 4525
BstC8I GCNNGC 9 cut(s) 487, 858, 2226, 2352, 2525, 3589, 3753, 4412, 4474
BstDSI CCRYGG 3 cut(s) 3381, 4213, 4426
BstENI CCTNNNNNAGG 1 cut(s) 3762
BstFNI CGCG 5 cut(s) 44, 46, 949, 3383, 4335
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 6 cut(s) 46, 994, 1560, 2332, 2703, 2961
BstMAI GTCTC 7 cut(s) 694, 1014, 1483, 2547, 2596, 2801, 3498
BstNSI RCATGY 3 cut(s) 489, 2775, 4648
BstSFI CTRYAG 3 cut(s) 369, 1543, 2250
BstSLI GKGCMC 5 cut(s) 53, 439, 1206, 1285, 4126
BstUI CGCG 5 cut(s) 44, 46, 949, 3383, 4335
BstV2I GAAGAC 4 cut(s) 824, 1455, 3266, 4698
BstX2I RGATCY 6 cut(s) 1097, 2128, 2581, 2946, 3617, 3949
BstXI CCANNNNNNTGG 2 cut(s) 245, 4406
BstYI RGATCY 6 cut(s) 1097, 2128, 2581, 2946, 3617, 3949
BstZ17I GTATAC 1 cut(s) 182
BsuI GTATCC 3 cut(s) 1952, 4261, 4736
BtgI CCRYGG 3 cut(s) 3381, 4213, 4426
BtrI CACGTC 1 cut(s) 40
BtsI GCAGTG 3 cut(s) 2247, 3460, 3842
BtsIMutI CAGTG 8 cut(s) 2056, 2247, 2878, 2913, 3294, 3460, 3842, 3998
BveI ACCTGC 3 cut(s) 2490, 3239, 3742
Cac8I GCNNGC 9 cut(s) 487, 858, 2226, 2352, 2525, 3589, 3753, 4412, 4474
CaiI CAGNNNCTG 1 cut(s) 1109
CfoI GCGC 6 cut(s) 46, 994, 1560, 2332, 2703, 2961
Cfr10I RCCGGY 2 cut(s) 2340, 4410
Cfr42I CCGCGG 1 cut(s) 3384
CseI GACGC 5 cut(s) 1095, 2873, 3507, 4184, 4289
CsiI ACCWGGT 2 cut(s) 3763, 3921
Csp6I GTAC 6 cut(s) 249, 511, 669, 1534, 2045, 3761
CspAI ACCGGT 1 cut(s) 2340
CviQI GTAC 6 cut(s) 249, 511, 669, 1534, 2045, 3761
DraI TTTAAA 1 cut(s) 2685
EaeI YGGCCR 4 cut(s) 217, 2556, 4408, 4713
Eam1104I CTCTTC 5 cut(s) 810, 2436, 3289, 4398, 4500
EarI CTCTTC 5 cut(s) 810, 2436, 3289, 4398, 4500
Eco130I CCWWGG 5 cut(s) 2537, 2717, 3312, 4126, 4426
Eco24I GRGCYC 1 cut(s) 2421
Eco31I GGTCTC 3 cut(s) 694, 2547, 2801
Eco32I GATATC 2 cut(s) 175, 4345
Eco47I GGWCC 7 cut(s) 1688, 1841, 2609, 3023, 3785, 4005, 4324
Eco47III AGCGCT 1 cut(s) 993
Eco57I CTGAAG 7 cut(s) 732, 1355, 1366, 2033, 3270, 3426, 4400
Eco72I CACGTG 1 cut(s) 824
Eco88I CYCGRG 1 cut(s) 1836
EcoNI CCTNNNNNAGG 1 cut(s) 3762
EcoO109I RGGNCCY 4 cut(s) 97, 3023, 4005, 4853
EcoRI GAATTC 1 cut(s) 4138
EcoRV GATATC 2 cut(s) 175, 4345
EcoT14I CCWWGG 5 cut(s) 2537, 2717, 3312, 4126, 4426
EcoT22I ATGCAT 1 cut(s) 2773
EcoT38I GRGCYC 1 cut(s) 2421
ErhI CCWWGG 5 cut(s) 2537, 2717, 3312, 4126, 4426
FaqI GGGAC 3 cut(s) 192, 430, 1674
FauI CCCGC 2 cut(s) 39, 3253
FauNDI CATATG 1 cut(s) 1194
FbaI TGATCA 1 cut(s) 3157
FblI GTMKAC 2 cut(s) 181, 3521
FriOI GRGCYC 1 cut(s) 2421
FspBI CTAG 7 cut(s) 705, 1050, 2132, 2706, 4316, 4434, 4469
GlaI GCGC 6 cut(s) 45, 993, 1559, 2331, 2702, 2960
GsaI CCCAGC 1 cut(s) 3597
GsuI CTGGAG 7 cut(s) 47, 633, 1388, 1488, 1752, 3639, 4382
HaeII RGCGCY 1 cut(s) 995
HapII CCGG 4 cut(s) 2341, 2755, 4411, 4850
HgaI GACGC 5 cut(s) 1095, 2873, 3507, 4184, 4289
HhaI GCGC 6 cut(s) 46, 994, 1560, 2332, 2703, 2961
Hin1I GRCGYC 1 cut(s) 2884
Hin6I GCGC 6 cut(s) 44, 992, 1558, 2330, 2701, 2959
HinP1I GCGC 6 cut(s) 44, 992, 1558, 2330, 2701, 2959
HincII GTYRAC 4 cut(s) 147, 1902, 4642, 4734
HindII GTYRAC 4 cut(s) 147, 1902, 4642, 4734
HindIII AAGCTT 5 cut(s) 599, 1124, 2474, 3234, 4302
HpaII CCGG 4 cut(s) 2341, 2755, 4411, 4850
Hpy99I CGWCG 5 cut(s) 16, 44, 107, 113, 134
HpyCH4IV ACGT 3 cut(s) 39, 513, 823
HpySE526I ACGT 3 cut(s) 39, 513, 823
Hsp92I GRCGYC 1 cut(s) 2884
HspAI GCGC 6 cut(s) 44, 992, 1558, 2330, 2701, 2959
KroI GCCGGC 1 cut(s) 4410
KroNI GCCGGC 1 cut(s) 4412
Ksp22I TGATCA 1 cut(s) 3157
KspI CCGCGG 1 cut(s) 3384
MabI ACCWGGT 2 cut(s) 3763, 3921
MaeI CTAG 7 cut(s) 705, 1050, 2132, 2706, 4316, 4434, 4469
MaeII ACGT 3 cut(s) 39, 513, 823
MaeIII GTNAC 8 cut(s) 403, 774, 781, 787, 3289, 3486, 4387, 4754
MbiI CCGCTC 1 cut(s) 4226
MfeI CAATTG 2 cut(s) 882, 3513
MflI RGATCY 6 cut(s) 1097, 2128, 2581, 2946, 3617, 3949
MhlI GDGCHC 7 cut(s) 53, 439, 1206, 1285, 2421, 4126, 4372
MlsI TGGCCA 2 cut(s) 219, 2558
MluNI TGGCCA 2 cut(s) 219, 2558
MlyI GAGTC 5 cut(s) 1907, 2968, 3275, 4063, 4431
MmeI TCCRAC 4 cut(s) 294, 388, 2753, 4692
Mox20I TGGCCA 2 cut(s) 219, 2558
Mph1103I ATGCAT 1 cut(s) 2773
MroNI GCCGGC 1 cut(s) 4410
MroXI GAANNNNTTC 4 cut(s) 1882, 2430, 3563, 4305
MscI TGGCCA 2 cut(s) 219, 2558
MslI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2212, 3165
Msp20I TGGCCA 2 cut(s) 219, 2558
MspA1I CMGCKG 4 cut(s) 878, 3383, 3440, 3782
MspCI CTTAAG 2 cut(s) 2950, 3628
MspI CCGG 4 cut(s) 2341, 2755, 4411, 4850
MunI CAATTG 2 cut(s) 882, 3513
Mva1269I GAATGC 2 cut(s) 295, 4131
MvnI CGCG 5 cut(s) 44, 46, 949, 3383, 4335
NaeI GCCGGC 1 cut(s) 4412
NciI CCSGG 1 cut(s) 4851
NcoI CCATGG 1 cut(s) 4426
NdeI CATATG 1 cut(s) 1194
NgoMIV GCCGGC 1 cut(s) 4410
NmeAIII GCCGAG 2 cut(s) 1273, 3127
NmuCI GTSAC 3 cut(s) 3289, 4387, 4754
NsiI ATGCAT 1 cut(s) 2773
NspI RCATGY 3 cut(s) 489, 2775, 4648
NspV TTCGAA 1 cut(s) 4525
PaeI GCATGC 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 3239
PciI ACATGT 1 cut(s) 4644
PctI GAATGC 2 cut(s) 295, 4131
PdiI GCCGGC 1 cut(s) 4412
PdmI GAANNNNTTC 4 cut(s) 1882, 2430, 3563, 4305
PfoI TCCNGGA 3 cut(s) 3853, 4198, 4398
PinAI ACCGGT 1 cut(s) 2340
PleI GAGTC 5 cut(s) 1906, 2968, 3275, 4063, 4430
PmaCI CACGTG 1 cut(s) 824
PmlI CACGTG 1 cut(s) 824
PpsI GAGTC 5 cut(s) 1906, 2968, 3275, 4063, 4430
Ppu21I YACGTR 1 cut(s) 824
PpuMI RGGWCCY 2 cut(s) 3023, 4005
PscI ACATGT 1 cut(s) 4644
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 2 cut(s) 3023, 4005
PspCI CACGTG 1 cut(s) 824
PspFI CCCAGC 1 cut(s) 3593
PspPPI RGGWCCY 2 cut(s) 3023, 4005
PsrI GAACNNNNNNTAC 2 cut(s) 2037, 2069
PstI CTGCAG 1 cut(s) 2254
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 6 cut(s) 1097, 2128, 2581, 2946, 3617, 3949
PvuII CAGCTG 3 cut(s) 878, 3440, 3782
RsaI GTAC 6 cut(s) 250, 512, 670, 1535, 2046, 3762
RsaNI GTAC 6 cut(s) 249, 511, 669, 1534, 2045, 3761
RseI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2212, 3165
SacII CCGCGG 1 cut(s) 3384
SchI GAGTC 5 cut(s) 1907, 2968, 3275, 4063, 4431
SduI GDGCHC 7 cut(s) 53, 439, 1206, 1285, 2421, 4126, 4372
SexAI ACCWGGT 2 cut(s) 3763, 3921
SfcI CTRYAG 3 cut(s) 369, 1543, 2250
Sfr303I CCGCGG 1 cut(s) 3384
SfuI TTCGAA 1 cut(s) 4525
SgrBI CCGCGG 1 cut(s) 3384
SinI GGWCC 7 cut(s) 1688, 1841, 2609, 3023, 3785, 4005, 4324
SmiMI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2212, 3165
SmlI CTYRAG 6 cut(s) 2601, 2950, 2976, 3628, 3975, 4259
SmoI CTYRAG 6 cut(s) 2601, 2950, 2976, 3628, 3975, 4259
SphI GCATGC 1 cut(s) 489
SspI AATATT 2 cut(s) 1152, 2094
SspMI CTAG 7 cut(s) 705, 1050, 2132, 2706, 4316, 4434, 4469
StyI CCWWGG 5 cut(s) 2537, 2717, 3312, 4126, 4426
TaiI ACGT 3 cut(s) 42, 516, 826
TatI WGTACW 1 cut(s) 2044
TauI GCSGC 7 cut(s) 77, 80, 1043, 2884, 3386, 4226, 4715
TscAI CASTG 8 cut(s) 2063, 2254, 2878, 2920, 3294, 3460, 3849, 4005
TseFI GTSAC 3 cut(s) 3289, 4387, 4754
Tsp45I GTSAC 3 cut(s) 3289, 4387, 4754
TspGWI ACGGA 1 cut(s) 3923
TspRI CASTG 8 cut(s) 2063, 2254, 2878, 2920, 3294, 3460, 3849, 4005
Vha464I CTTAAG 2 cut(s) 2950, 3628
VpaK11BI GGWCC 7 cut(s) 1688, 1841, 2609, 3023, 3785, 4005, 4324
VspI ATTAAT 1 cut(s) 834
XagI CCTNNNNNAGG 1 cut(s) 3762
XapI RAATTY 9 cut(s) 862, 982, 1375, 1580, 2159, 2693, 3413, 4138, 4772
XbaI TCTAGA 2 cut(s) 2131, 2705
XceI RCATGY 3 cut(s) 489, 2775, 4648
XcmI CCANNNNNNNNNTGG 2 cut(s) 639, 982
XmiI GTMKAC 2 cut(s) 181, 3521
XmnI GAANNNNTTC 4 cut(s) 1882, 2430, 3563, 4305
XspI CTAG 7 cut(s) 705, 1050, 2132, 2706, 4316, 4434, 4469
Zsp2I ATGCAT 1 cut(s) 2773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.