FvH4_1g15690

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008489
Physical Location & Seq
Forward (+)
8907227 .. 8919153
11927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15690.t3

Sequence Viewer

Length: 4863 bp
ATGGAGTGGACGACGGTGCAGCATCTGGATCTACGACACGTCGCGCGGAGCACCAAGCCGCTGCAGCCTCATGCCGCCGCGTTCCATCCGCATCAGGCGCTCATCGCCGTCGCTATCGGCAACTACATCGTCGAAATGGATGCGTTGACGGGATGCAAGATTGCATCTATTGATATCGGTGTACCTGTTATCCGCATGGCATATAGTCCCACAAGTGGGCATGCTGTCATAGCGATCCATGAGGATGGTACCATTCGCTCCTGTGATTTTGATGCCGAACAAACTTGTGTGTTGCATTCACCTGAGAAAAAGTTGGATCAAATTACTCCTGATACAGAAGTCCATCTTGCTTTGACACCACTCCAACCAGTAGTGTTTTTCGGATTCCATAAAAGGATGAGTGTTACAGTTGTTGGGACTGTTGAAGGAGGAAGGGCACCAACAAAAATAAAGACAGATTTGAAAAAACCTATTGTGAATCTTGCATGCCACCCTCGCCATCCTGTCCTGTATGTAGCCTATGCAGATGGTTTGATTCGAGCATACAATATTCACACATATGCAGTTCATTACACCTTACAGATTGACAACACCATAAAGCTTATCGGTGCCGGTGCGTTTGGTTTTCACCCAACATTGGAGTGGATTTTTGTTGGCGATAGACGGGGTACACTTCTTGCATGGGATGTTTCAACTGAGAGACCTAATATGATTGGAATTACACAAGTGGGTTCCCAACCAATTTCTTCAGTTTCTTGGCTACCAATGTTGCGGTTACTGGTAACTGTAACCAGGGATGGAACTCTTCAAGTTTGGAAGACGCGTGTTATAATTAATCCCAACAGACCTCCAATGCAAGCAAATTTCTTTGAACCTGCTGCAATTGAACCACTCGATATCCCTCGCATACTTTCTCAACAGGGAGGAGAAGCAGCTTATCCTTTACCGCGTATCAAAACATTAGAAGTTCACTCCAAACTGAATCTGGCAGCACTTTTGTTCATAAATATGGCAGGTGCAGACAATGTGAAGAATAGAGCCGCATACACTAGGGAAGGAAGGAAACAACTGTTCGCAGTTTTGCAAGGGGCAAGAGGATCTTCAGCATCTGTTTTGAAAGAAAAGCTTTCATCGTTGGGATCATCTGGAATATTAGCTGAACATCAACTTCAAGCTCAATTACAAGAACATCATATGAAAGGGCACAGTCAGCTTACAATATCAGACATTGCTCGGAAGGCTTTTCTCCACAGTCATTTCATGGAAGGCCATGCCAAAAGTGCCCCCATATCTCGGCTGCCTCTAATTACTATTGTAGATTCTAAGCATCATCTGAAGGATGCTCCTGTATGTCAGCCGTTTCACCTGGAGCTGAATTTCTTCAGTAAAGAGAATCGGGTTCTTCATTATCCTGTCAGAGCCTTTTGTATAGATGGATCAAACCTTATGGCATATAATCTGTGTTCTGGAGCAGATAGTATCTACAAGAGACTTCATACATCGGTTCCTGCAAACGTGGAATACCATCCAAAGTACCTGTTCTACAGTAAAAAACAGCACATATTTCTTGTTGTGTATGAATTTAGTGGTGCTACAAATGAAGTTGTGCTTTACTTTGAAAATTCTGATTCTCAGGCAGCAAATAGTAAATGCACCACCATAAAAGGTCGAGATGCAGCATTTATTGGCCCCAACGAAAATCAGTTTGCAATTCTTGATGATGATAAGACTGGACTGGCTTTACATATCCTGCCTGGAAAGGCTACTCCAGAAGCTAATGAAAAAAATCTACTCGCTGATGAAAACCAATCTATGAATACCGAAACCAGTGCCCCCCAAGGTCCGATGCAATTTTTGTTCGAAACTGAAGTTGATCGAATCTTTTCCACTCCAATAGAGTCAACTCTGATGTTTGCTTCTCATGGGGATCAGATTGGCTTGGCAAAGCTGGTTCAAGGATACCGCCTTTCTAATGCTGGCGGTCATTATATAGCAACAACAAATGAAGGAAGAAAGTCAATCAAATTGAAACTCAATGAAATTGTACTTCAGGTTCACTGGCAAGAAACTCTTAGGGGCTATGTTGCAGGAATATTAACTACACAAAGAGTGCTTATAGTTTCGGCAGATCTAGATATACTGGCAGGAAGTTCTGCAAGATTTGATAAAGGGCTTCCTTCATTTAGATCGCTTTTATGGGTTGGGCCTGCGCTTCTCTTTTCTACAACAACTGCAGTTAGTGTGCTCGGTTGGGATGGGAAAGTGAGGACTATACTCTCTATCAGTATGCCGTATGCAGTCTTGATTGGTGCGCTGAATGACCGCTTGTTGCTTGCTACCCCAACAGAGATAAATCCCAGACAAAAGAAGGGAGTTGAGATTAAGAGTTGTCTTGTTGGGCTCCTTGAACCTCTTCTTATTGGATTTGCCACAATGCAAGAAAGATTTGAGCAGAAGCTTGACCTGCCAGAGATATTGTATCAAATAACATCAAGGTTTGATAGCCTGCGTATCACTCCAAGGTCTCTTGATATTCTTGCCAGAGGATCTCCTGTTTGTGGAGATCTTTCTGTCTCATTATCACAAGCAGGACCACAGTTCACACAGGTGTTGCGTGGTGTCTATGCTATCAAAGCTCTCCGCTTTTCTACTGCTTTATCTGTTTTGAAAGATGAGTTTCTGCGCTCTAGAGATTATCCAAGGTGTCCTCCAACATCTCATTTGTTCCACCGGTTCCGGCAGTTGGGCTATGCATGTATCAAGTTTGGTCAGTTTGATAGTGCAAAAGAGACCTTTGAAGTTATAGCAGATTATGAAAGTATGCTCGATCTGTTTATCTGCCACCTTAACCCCAGTGCCATGCGTCGTCTTGCTCAAAAATTGGAAGAAGATGGCACTGATTCAGAACTAAGGAGATACTGTGAGAGGATTTTAAGAGTCCGCTCTACTGGATGGACTCAAGGCATTTTCGCTAACTTCGCTGCTGAGAGTATGGTTCCAAAAGGTCCTGAATGGGGTGGTGGGAACTGGGAAATAAAAACCCCAACTAACATGAAGGCAATACCTCAGTGGGAACTGGCTGCCGAGGTGATGCCATACATGAGGACTGATGACGGTCCCATCCCATCTATTATTGCTGATCATATTGGTGTTTACTTGGGTTCCATCAGAGGACGAGGCAACATCGTAGAAGTAAGGGAAGATAGCTTGGTCAAAGCTTTCAAATCCGCAGGTGGTGACAATAAGCCAAATGGAGTTCAGGACTCTTCTGTGAAATCTGCATCTGATGTGTCCAAGGGAGTGCCTGGTGGTGGTTCGTTGATGGGTCTTGAAACACTTACCAAACAAGTAGCCAGTTCCACTGTGGCAGATGAACAGGCTAAAGCAGAAGAAGAATTCAAGAAATCAATGTATGGTACAGCTGATGGTAGCAGCAGTGATGAGGAGGGAACTTCAAAAGCTAAAAAATTACGTATTCGGATAAGAGACAAACCTGTTACTTCTACCACAGTGGATCTTGATAAGATCAAAGAAGCCACAAAGCAATTCAAACTTGGTGAGGGGTTGGCTCGTCCTAGCAGAACCAAGTCATTGACCGGATCTCAGGATCTTAGCCAGATATTATCCCAGCCACCAGCCAATAGTGGTTTTCCAAATGTCCGGGTAGGTTCTGCCCCTGGTGATCTGTTTGGTATGGATGCACTAACACAACCTGCAACTGTATCACAGCAAGCTCCTACGGCACCAGGTGTGGGAATGACTGCTAGACCAATTCCAGAGGACTTTTTCCAAAATACCATACCGTCCCTCCAAGTTGCAGCCTCACTGCCCCCTCCTGGAACGTATCTTTCAAGGATGGAGCAAGCTTCTCAGGGGGTTGAAAGAAATACGGAAACATTTAACCAGGTCAATGCACCCAAACCCAATATTGATCTTCCTGATGGTGGTGTTCCCCCTCAAGCTACTCAACAGGGTGTTCCACTGGAGTCCTATGGACTTCCTGATGGTGGGGTTCCACCACAAGCCCCACGCCAGGCTGCTATTCAGCAACGGACTCAGATTCAATCAGCTCAACCTCCAATATCTACTCAGCCTCTTGATCTTAGTGCCCTTGGCATTCCGAATTCTGCTGATAATGGAAAACCTTCCGGGCAGCCTCCTTCACCTCCATCTGCTGTGCGTCCTGGACAGGTTCCCCGTGGTGCAGCTGCTACTACATGCTTTAAGACTGGAGTATCCCACCTTGAGCAGAATCAACTTTCGGATGCATTATCCTGCTTCGATGAAGCTTTTCTTGCATTAGCTAAGGACAACTCTCGCGGAGCTGATATAAAAGCACAAGCCACCATATGTGCTCAATACAAGATAGCAGTCACTCTTCTTCAGGAAATCGGGCGGCTTCAGAGAGTCCACGGCCCTAGTGCAATTAGCGCAAAAGATGAGATGGCAAGACTATCACGCCACCTGGGTTCTTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATTCGAACTGCTATCAAGAGGAATATGGAAGTGCAGAATTACGCTTATTCAAAACAGATGCTTGAACTCCTGCTGTCGAAAGCACCTCCAAGTAAACAGGATGAACTAAGGAGCTTAGTTGACATGTGTGTTCAAAGGGGTCTTTCCAACAAGTCCATTGATCCTCTTGAAGACCCTTCTCAGTTCTGTGCTGCCACACTTAGTCGTCTGTCAACCATTGGATACGATGTTTGTGACCTTTGTGGGGCCAAATTTTCGGCTCTCGCAACGCCTGGTTGCATTATCTGCGGTATGGGAAGCATCAAAAGATCAGATGCACTTACAGGGCCGGGGCCTGTTCCTTCGCCGTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

1621

Amino Acids

176.61

Weight (kDa)

8.54

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 108 - 227 9.4e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 110 - 230 3.6e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 110 - 232 1.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 116 - 229 6e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AarI CACCTGC 2 cut(s) 1004, 3221
Acc36I ACCTGC 5 cut(s) 883, 1004, 2472, 3221, 3721
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 4 cut(s) 248, 436, 608, 3742
AccBSI CCGCTC 1 cut(s) 2943
AccII CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
AcsI RAATTY 7 cut(s) 862, 1375, 1580, 1621, 3395, 4123, 4757
AcuI CTGAAG 8 cut(s) 732, 1086, 1355, 1366, 1887, 2033, 4367, 4385
AdeI CACNNNGTG 1 cut(s) 3749
AfaI GTAC 6 cut(s) 183, 250, 670, 1535, 2046, 3418
AflIII ACRYGT 3 cut(s) 37, 821, 4629
AgeI ACCGGT 1 cut(s) 2730
AjiI CACGTC 1 cut(s) 40
AleI CACNNNNGTG 1 cut(s) 2606
Alw21I GWGCWC 3 cut(s) 53, 2247, 4357
Alw26I GTCTC 6 cut(s) 694, 1483, 2529, 2578, 2783, 3480
AlwNI CAGNNNCTG 2 cut(s) 25, 1109
AoxI GGCC 7 cut(s) 1267, 1687, 2204, 4414, 4752, 4832, 4838
ApoI RAATTY 7 cut(s) 862, 1375, 1580, 1621, 3395, 4123, 4757
AseI ATTAAT 1 cut(s) 834
AsiGI ACCGGT 1 cut(s) 2730
Asp700I GAANNNNTTC 5 cut(s) 1379, 1882, 2412, 4144, 4290
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 6 cut(s) 46, 100, 2212, 2314, 2685, 4433
AsuC2I CCSGG 3 cut(s) 3662, 4150, 4836
AsuHPI GGTGA 8 cut(s) 291, 620, 1355, 3100, 3248, 3569, 3692, 4155
AsuII TTCGAA 2 cut(s) 1860, 4510
AvaII GGWCC 4 cut(s) 1841, 2591, 3005, 3116
BaeGI GKGCMC 5 cut(s) 439, 1206, 1285, 1834, 4111
BaeI ACNNNNGTAYC 4 cut(s) 324, 357, 4218, 4251
BanI GGYRCC 4 cut(s) 248, 436, 608, 3742
BanII GRGCYC 1 cut(s) 2403
BbsI GAAGAC 2 cut(s) 824, 4683
Bbv12I GWGCWC 3 cut(s) 53, 2247, 4357
BceAI ACGGC 6 cut(s) 92, 1342, 2275, 3756, 4429, 4837
BcgI CGANNNNNNTGC 6 cut(s) 109, 122, 143, 156, 1811, 1845
BciVI GTATCC 3 cut(s) 1952, 4246, 4721
BclI TGATCA 1 cut(s) 3139
BcnI CCSGG 3 cut(s) 3662, 4150, 4836
BcoDI GTCTC 6 cut(s) 694, 1483, 2529, 2578, 2783, 3480
BfaI CTAG 6 cut(s) 1050, 2132, 2688, 3576, 3765, 4419
BfmI CTRYAG 3 cut(s) 62, 1543, 2232
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 5 cut(s) 883, 1004, 2472, 3221, 3721
BfuI GTATCC 3 cut(s) 1952, 4246, 4721
BglII AGATCT 2 cut(s) 2128, 2563
Bme18I GGWCC 4 cut(s) 1841, 2591, 3005, 3116
BmgBI CACGTC 1 cut(s) 40
BmrI ACTGGG 2 cut(s) 2847, 3037
BmuI ACTGGG 2 cut(s) 2847, 3037
BpiI GAAGAC 2 cut(s) 824, 4683
BpmI CTGGAG 5 cut(s) 1388, 1488, 1752, 4004, 4251
Bpu10I CCTNAGC 1 cut(s) 4305
Bpu14I TTCGAA 2 cut(s) 1860, 4510
BpuEI CTTGAG 3 cut(s) 2943, 3942, 4265
BpuMI CCSGG 3 cut(s) 3662, 4150, 4836
BsaAI YACGTR 1 cut(s) 3473
BsaI GGTCTC 3 cut(s) 694, 2529, 2783
BsaWI WCCGGW 2 cut(s) 2730, 3596
BsaXI ACNNNNNCTCC 4 cut(s) 2905, 2935, 3792, 3822
Bse118I RCCGGY 2 cut(s) 611, 2730
Bse3DI GCAATG 1 cut(s) 1227
BseMI GCAATG 1 cut(s) 1227
BseRI GAGGAG 2 cut(s) 939, 3458
BseSI GKGCMC 5 cut(s) 439, 1206, 1285, 1834, 4111
BseYI CCCAGC 1 cut(s) 3627
BsgI GTGCAG 4 cut(s) 38, 1038, 4224, 4559
Bsh1236I CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
BshFI GGCC 7 cut(s) 1269, 1689, 2206, 4416, 4754, 4834, 4840
BshNI GGYRCC 4 cut(s) 248, 436, 608, 3742
BshTI ACCGGT 1 cut(s) 2730
BsiHKAI GWGCWC 3 cut(s) 53, 2247, 4357
BsiSI CCGG 7 cut(s) 612, 2731, 2737, 3597, 3661, 4149, 4835
BslFI GGGAC 4 cut(s) 192, 430, 3102, 3790
BsmAI GTCTC 6 cut(s) 694, 1483, 2529, 2578, 2783, 3480
BsmFI GGGAC 4 cut(s) 192, 430, 3102, 3790
BsmI GAATGC 2 cut(s) 295, 4116
BsnI GGCC 7 cut(s) 1269, 1689, 2206, 4416, 4754, 4834, 4840
Bso31I GGTCTC 3 cut(s) 694, 2529, 2783
Bsp119I TTCGAA 2 cut(s) 1860, 4510
Bsp1286I GDGCHC 9 cut(s) 53, 439, 1206, 1285, 1834, 2247, 2403, 4111, 4357
BspANI GGCC 7 cut(s) 1269, 1689, 2206, 4416, 4754, 4834, 4840
BspFNI CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
BspMAI CTGCAG 2 cut(s) 66, 2236
BspMI ACCTGC 5 cut(s) 883, 1004, 2472, 3221, 3721
BspT104I TTCGAA 2 cut(s) 1860, 4510
BspT107I GGYRCC 4 cut(s) 248, 436, 608, 3742
BspTNI GGTCTC 3 cut(s) 694, 2529, 2783
BsrBI CCGCTC 1 cut(s) 2943
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 2 cut(s) 611, 2730
BssAI RCCGGY 2 cut(s) 611, 2730
BssT1I CCWWGG 5 cut(s) 1837, 2519, 2699, 3294, 4111
Bst6I CTCTTC 5 cut(s) 810, 2418, 3271, 4383, 4485
BstAPI GCANNNNNTGC 1 cut(s) 4791
BstBAI YACGTR 1 cut(s) 3473
BstBI TTCGAA 2 cut(s) 1860, 4510
BstC8I GCNNGC 9 cut(s) 222, 487, 858, 1978, 2208, 2334, 2507, 3732, 3864
BstDSI CCRYGG 2 cut(s) 4198, 4411
BstFNI CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 6 cut(s) 46, 100, 2212, 2314, 2685, 4433
BstMAI GTCTC 6 cut(s) 694, 1483, 2529, 2578, 2783, 3480
BstNSI RCATGY 5 cut(s) 224, 489, 2757, 4221, 4633
BstSFI CTRYAG 3 cut(s) 62, 1543, 2232
BstSLI GKGCMC 5 cut(s) 439, 1206, 1285, 1834, 4111
BstSNI TACGTA 1 cut(s) 3473
BstUI CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
BstV2I GAAGAC 2 cut(s) 824, 4683
BstX2I RGATCY 8 cut(s) 28, 1097, 2128, 2546, 2563, 3514, 3599, 3607
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 8 cut(s) 28, 1097, 2128, 2546, 2563, 3514, 3599, 3607
BsuI GTATCC 3 cut(s) 1952, 4246, 4721
BsuRI GGCC 7 cut(s) 1269, 1689, 2206, 4416, 4754, 4834, 4840
BtgI CCRYGG 2 cut(s) 4198, 4411
BtgZI GCGATG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 40
BtsI GCAGTG 2 cut(s) 3442, 3824
BveI ACCTGC 5 cut(s) 883, 1004, 2472, 3221, 3721
Cac8I GCNNGC 9 cut(s) 222, 487, 858, 1978, 2208, 2334, 2507, 3732, 3864
CaiI CAGNNNCTG 2 cut(s) 25, 1109
CfoI GCGC 6 cut(s) 46, 100, 2212, 2314, 2685, 4433
Cfr10I RCCGGY 2 cut(s) 611, 2730
CseI GACGC 3 cut(s) 829, 2852, 4169
CsiI ACCWGGT 2 cut(s) 3745, 3903
Csp6I GTAC 6 cut(s) 182, 249, 669, 1534, 2045, 3417
CspAI ACCGGT 1 cut(s) 2730
CviQI GTAC 6 cut(s) 182, 249, 669, 1534, 2045, 3417
DraIII CACNNNGTG 1 cut(s) 3749
Eam1104I CTCTTC 5 cut(s) 810, 2418, 3271, 4383, 4485
EarI CTCTTC 5 cut(s) 810, 2418, 3271, 4383, 4485
Eco105I TACGTA 1 cut(s) 3473
Eco130I CCWWGG 5 cut(s) 1837, 2519, 2699, 3294, 4111
Eco24I GRGCYC 1 cut(s) 2403
Eco31I GGTCTC 3 cut(s) 694, 2529, 2783
Eco32I GATATC 2 cut(s) 175, 898
Eco47I GGWCC 4 cut(s) 1841, 2591, 3005, 3116
Eco57I CTGAAG 8 cut(s) 732, 1086, 1355, 1366, 1887, 2033, 4367, 4385
EcoO109I RGGNCCY 2 cut(s) 3005, 4838
EcoRI GAATTC 2 cut(s) 3395, 4123
EcoRV GATATC 2 cut(s) 175, 898
EcoT14I CCWWGG 5 cut(s) 1837, 2519, 2699, 3294, 4111
EcoT22I ATGCAT 2 cut(s) 2755, 4270
EcoT38I GRGCYC 1 cut(s) 2403
ErhI CCWWGG 5 cut(s) 1837, 2519, 2699, 3294, 4111
FaqI GGGAC 4 cut(s) 192, 430, 3102, 3790
FauNDI CATATG 3 cut(s) 559, 1194, 4349
FbaI TGATCA 1 cut(s) 3139
FriOI GRGCYC 1 cut(s) 2403
FspBI CTAG 6 cut(s) 1050, 2132, 2688, 3576, 3765, 4419
GlaI GCGC 6 cut(s) 45, 99, 2211, 2313, 2684, 4432
GsaI CCCAGC 1 cut(s) 3631
GsuI CTGGAG 5 cut(s) 1388, 1488, 1752, 4004, 4251
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 7 cut(s) 1269, 1689, 2206, 4416, 4754, 4834, 4840
HapII CCGG 7 cut(s) 612, 2731, 2737, 3597, 3661, 4149, 4835
HgaI GACGC 3 cut(s) 829, 2852, 4169
HhaI GCGC 6 cut(s) 46, 100, 2212, 2314, 2685, 4433
Hin6I GCGC 6 cut(s) 44, 98, 2210, 2312, 2683, 4431
HinP1I GCGC 6 cut(s) 44, 98, 2210, 2312, 2683, 4431
HincII GTYRAC 4 cut(s) 147, 1902, 4627, 4719
HindII GTYRAC 4 cut(s) 147, 1902, 4627, 4719
HindIII AAGCTT 6 cut(s) 599, 1124, 2456, 3216, 3864, 4287
HpaII CCGG 7 cut(s) 612, 2731, 2737, 3597, 3661, 4149, 4835
HphI GGTGA 8 cut(s) 291, 620, 1355, 3100, 3248, 3569, 3692, 4155
Hpy99I CGWCG 5 cut(s) 16, 44, 113, 134, 2868
HpyCH4IV ACGT 4 cut(s) 39, 1515, 3472, 3842
HpySE526I ACGT 4 cut(s) 39, 1515, 3472, 3842
HspAI GCGC 6 cut(s) 44, 98, 2210, 2312, 2683, 4431
KpnI GGTACC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 3139
MabI ACCWGGT 2 cut(s) 3745, 3903
MaeI CTAG 6 cut(s) 1050, 2132, 2688, 3576, 3765, 4419
MaeII ACGT 4 cut(s) 39, 1515, 3472, 3842
MaeIII GTNAC 8 cut(s) 403, 774, 781, 787, 3236, 3496, 4372, 4739
MbiI CCGCTC 1 cut(s) 2943
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 8 cut(s) 28, 1097, 2128, 2546, 2563, 3514, 3599, 3607
MhlI GDGCHC 9 cut(s) 53, 439, 1206, 1285, 1834, 2247, 2403, 4111, 4357
MluI ACGCGT 1 cut(s) 821
MlyI GAGTC 7 cut(s) 1907, 2946, 2950, 3257, 3995, 4048, 4416
MmeI TCCRAC 4 cut(s) 294, 388, 2735, 4677
Mph1103I ATGCAT 2 cut(s) 2755, 4270
MroXI GAANNNNTTC 5 cut(s) 1379, 1882, 2412, 4144, 4290
MseI TTAA 7 cut(s) 834, 2096, 2382, 2847, 2933, 3900, 4224
MslI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2606, 3147
MspA1I CMGCKG 3 cut(s) 61, 3422, 4208
MspI CCGG 7 cut(s) 612, 2731, 2737, 3597, 3661, 4149, 4835
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 2 cut(s) 295, 4116
MvnI CGCG 6 cut(s) 44, 46, 80, 823, 949, 4320
NciI CCSGG 3 cut(s) 3662, 4150, 4836
NdeI CATATG 3 cut(s) 559, 1194, 4349
NmeAIII GCCGAG 2 cut(s) 1273, 3109
NmuCI GTSAC 3 cut(s) 3236, 4372, 4739
NsiI ATGCAT 2 cut(s) 2755, 4270
NspI RCATGY 5 cut(s) 224, 489, 2757, 4221, 4633
NspV TTCGAA 2 cut(s) 1860, 4510
OliI CACNNNNGTG 1 cut(s) 2606
PaeI GCATGC 2 cut(s) 224, 489
PaqCI CACCTGC 2 cut(s) 1004, 3221
PciI ACATGT 1 cut(s) 4629
PctI GAATGC 2 cut(s) 295, 4116
PdmI GAANNNNTTC 5 cut(s) 1379, 1882, 2412, 4144, 4290
PfoI TCCNGGA 2 cut(s) 3835, 4183
PinAI ACCGGT 1 cut(s) 2730
PleI GAGTC 7 cut(s) 1906, 2945, 2950, 3257, 3994, 4048, 4415
PpsI GAGTC 7 cut(s) 1906, 2945, 2950, 3257, 3994, 4048, 4415
Ppu21I YACGTR 1 cut(s) 3473
PpuMI RGGWCCY 1 cut(s) 3005
PscI ACATGT 1 cut(s) 4629
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 3005
PspFI CCCAGC 1 cut(s) 3627
PspPPI RGGWCCY 1 cut(s) 3005
PsrI GAACNNNNNNTAC 2 cut(s) 2037, 2069
PstI CTGCAG 2 cut(s) 66, 2236
PstNI CAGNNNCTG 2 cut(s) 25, 1109
PsuI RGATCY 8 cut(s) 28, 1097, 2128, 2546, 2563, 3514, 3599, 3607
PvuII CAGCTG 2 cut(s) 3422, 4208
RsaI GTAC 6 cut(s) 183, 250, 670, 1535, 2046, 3418
RsaNI GTAC 6 cut(s) 182, 249, 669, 1534, 2045, 3417
RseI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2606, 3147
SaqAI TTAA 7 cut(s) 834, 2096, 2382, 2847, 2933, 3900, 4224
SchI GAGTC 7 cut(s) 1907, 2946, 2950, 3257, 3995, 4048, 4416
SduI GDGCHC 9 cut(s) 53, 439, 1206, 1285, 1834, 2247, 2403, 4111, 4357
SexAI ACCWGGT 2 cut(s) 3745, 3903
SfcI CTRYAG 3 cut(s) 62, 1543, 2232
SfuI TTCGAA 2 cut(s) 1860, 4510
SinI GGWCC 4 cut(s) 1841, 2591, 3005, 3116
SmiMI CAYNNNNRTG 6 cut(s) 243, 558, 640, 1007, 2606, 3147
SmlI CTYRAG 3 cut(s) 2958, 3957, 4244
SmoI CTYRAG 3 cut(s) 2958, 3957, 4244
SnaBI TACGTA 1 cut(s) 3473
SphI GCATGC 2 cut(s) 224, 489
SspI AATATT 4 cut(s) 550, 1152, 2094, 3928
SspMI CTAG 6 cut(s) 1050, 2132, 2688, 3576, 3765, 4419
StyI CCWWGG 5 cut(s) 1837, 2519, 2699, 3294, 4111
TaiI ACGT 4 cut(s) 42, 1518, 3475, 3845
TatI WGTACW 1 cut(s) 2044
TauI GCSGC 5 cut(s) 61, 77, 80, 1043, 4399
Tru1I TTAA 7 cut(s) 834, 2096, 2382, 2847, 2933, 3900, 4224
Tru9I TTAA 7 cut(s) 834, 2096, 2382, 2847, 2933, 3900, 4224
TseFI GTSAC 3 cut(s) 3236, 4372, 4739
Tsp45I GTSAC 3 cut(s) 3236, 4372, 4739
TspGWI ACGGA 2 cut(s) 3905, 4066
VpaK11BI GGWCC 4 cut(s) 1841, 2591, 3005, 3116
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 7 cut(s) 862, 1375, 1580, 1621, 3395, 4123, 4757
XbaI TCTAGA 2 cut(s) 2131, 2687
XceI RCATGY 5 cut(s) 224, 489, 2757, 4221, 4633
XcmI CCANNNNNNNNNTGG 3 cut(s) 639, 982, 3361
XmnI GAANNNNTTC 5 cut(s) 1379, 1882, 2412, 4144, 4290
XspI CTAG 6 cut(s) 1050, 2132, 2688, 3576, 3765, 4419
Zsp2I ATGCAT 2 cut(s) 2755, 4270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.