AT3G50850

Protein N-lysine methyltransferase METTL21A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012035
Physical Location & Seq
Reverse (-)
18901342 .. 18902152
811 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50850.1

Sequence Viewer

Length: 756 bp
ATGGCTCTCCGAGAAGACGACGACGATGTTAACATCGGAGAAAAAGTAGTCGACGTAGAATCGGAGCTTCAGAAGTACCGGATCAATTCGATCGAATCAACGGTTGTGATTCGTCAGTTACCGACACAAGGCATTGCCTTCAAGCTCTGGATACCTGCAACAACTCTCGTCACGCTTTTAGATAACTACCGTCGTGACCCAAACATTAGTCCTCTCAATCGCACCTTCTCGAGTTTTCAATCCGACGGTTCAGATTCTTCTTCTCCGATTAACATCGTCGAGCTCGGATCTGGAACGGGAATCGTCGGTATCGCAGCGGCTGCGACACTAGGTGCTAATGTCACGGTGACGGATCTCCCAAACGTAATCGAGAATCTCAAATTCAACGCCGACGCAAACGCTCAAGTCGTGGCTAAGTTCGGTGGGAAAGTCCACGTGGCGTCTCTTCGTTGGGGCGAAATCGATGACGTGGAAAGTTTGGGTCAAAACGTTGACTTGATTCTAGCGTCTGACGTGGTTTATCACGTTCATCTTTATGAACCTCTCCTCAAAACGCTGCGTTTTCTGCTCTTAGAAGGATCATCCGAGAGAGTGTTTCTTATGGCTCACCTCAAGAGATGGAAGAAAGAATCGATTTTTTTCAAGAAAGCTCGGAGATTCTTCGATGTTGATGTTATACATTGTGATGATCCTCAAGAAGGTGCTAGAATCGGAGTTGTAGTTTACCGTTTTGCACCGAAAAATCAAAACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

251

Amino Acids

27.91

Weight (kDa)

5.43

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 34 - 218 2.5e-34 Lysine methyltransferase
Methyltransf_23 PF13489 86 - 208 5.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
AccI GTMKAC 1 cut(s) 51
AciI CCGC 1 cut(s) 317
AclI AACGTT 1 cut(s) 489
AclWI GGATC 5 cut(s) 89, 295, 360, 586, 683
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 1 cut(s) 53
AcvI CACGTG 1 cut(s) 436
AcyI GRCGYC 1 cut(s) 440
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 128, 698
AgsI TTSAA 4 cut(s) 142, 239, 385, 643
AjiI CACGTC 2 cut(s) 469, 514
AluBI AGCT 4 cut(s) 67, 145, 283, 650
AluI AGCT 4 cut(s) 67, 145, 283, 650
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 5 cut(s) 89, 295, 360, 586, 683
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 229
ApeKI GCWGC 3 cut(s) 314, 320, 556
ApoI RAATTY 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 358, 599
AvaI CYCGRG 1 cut(s) 229
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 307, 326, 543
BccI CCATC 1 cut(s) 612
BciVI GTATCC 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 3 cut(s) 329, 503, 705
BfuAI ACCTGC 1 cut(s) 163
BfuI GTATCC 1 cut(s) 144
BisI GCNGC 4 cut(s) 315, 318, 321, 557
BlsI GCNGC 4 cut(s) 316, 319, 322, 558
BmeT110I CYCGRG 1 cut(s) 229
BmgBI CACGTC 2 cut(s) 469, 514
BpiI GAAGAC 1 cut(s) 21
BpuEI CTTGAG 3 cut(s) 387, 596, 678
Bsa29I ATCGAT 2 cut(s) 462, 632
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 440
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 705, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 698
Bse3DI GCAATG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 272
BseCI ATCGAT 2 cut(s) 462, 632
BseGI GGATG 1 cut(s) 581
BseJI GATNNNNATC 1 cut(s) 272
BseLI CCNNNNNNNGG 2 cut(s) 128, 698
BseMI GCAATG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 3 cut(s) 307, 326, 543
Bsh1285I CGRYCG 1 cut(s) 93
BshVI ATCGAT 2 cut(s) 462, 632
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 229
BsiSI CCGG 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 128, 698
BsmAI GTCTC 1 cut(s) 447
BsmBI CGTCTC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 6 cut(s) 81, 90, 287, 352, 578, 688
BspACI CCGC 1 cut(s) 317
BspDI ATCGAT 2 cut(s) 462, 632
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 5 cut(s) 89, 295, 360, 586, 683
BsrDI GCAATG 1 cut(s) 132
BssMI GATC 6 cut(s) 81, 90, 287, 352, 578, 688
BssNI GRCGYC 1 cut(s) 440
Bst4CI ACNGT 5 cut(s) 103, 191, 248, 346, 728
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 436
BstDEI CTNAG 2 cut(s) 414, 571
BstENI CCTNNNNNAGG 1 cut(s) 696
BstF5I GGATG 1 cut(s) 581
BstKTI GATC 6 cut(s) 84, 93, 290, 355, 581, 691
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 6 cut(s) 81, 90, 287, 352, 578, 688
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 320, 565
BstV1I GCAGC 3 cut(s) 307, 326, 543
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 2 cut(s) 287, 352
BstYI RGATCY 2 cut(s) 287, 352
Bsu15I ATCGAT 2 cut(s) 462, 632
BsuI GTATCC 1 cut(s) 144
BsuTUI ATCGAT 2 cut(s) 462, 632
BtrI CACGTC 2 cut(s) 469, 514
BtsCI GGATG 1 cut(s) 581
BveI ACCTGC 1 cut(s) 163
CaiI CAGNNNCTG 1 cut(s) 320
ClaI ATCGAT 2 cut(s) 462, 632
CseI GACGC 3 cut(s) 401, 429, 495
Csp6I GTAC 1 cut(s) 76
CviJI RGCY 8 cut(s) 5, 67, 145, 283, 320, 413, 605, 650
CviKI_1 RGCY 8 cut(s) 5, 67, 145, 283, 320, 413, 605, 650
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 2 cut(s) 414, 571
DpnI GATC 6 cut(s) 83, 92, 289, 354, 580, 690
DpnII GATC 6 cut(s) 81, 90, 287, 352, 578, 688
DraIII CACNNNGTG 1 cut(s) 332
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 53
Eco72I CACGTG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 447
FaiI YATR 3 cut(s) 537, 602, 677
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 4 cut(s) 315, 318, 321, 557
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 4 cut(s) 315, 318, 321, 557
FspBI CTAG 3 cut(s) 329, 503, 705
GluI GCNGC 4 cut(s) 315, 318, 321, 557
HapII CCGG 1 cut(s) 79
HgaI GACGC 3 cut(s) 401, 429, 495
Hin1I GRCGYC 1 cut(s) 440
HincII GTYRAC 3 cut(s) 31, 52, 493
HindII GTYRAC 3 cut(s) 31, 52, 493
HpaI GTTAAC 1 cut(s) 31
HpaII CCGG 1 cut(s) 79
HphI GGTGA 2 cut(s) 358, 599
Hpy166II GTNNAC 5 cut(s) 31, 52, 433, 493, 724
Hpy188III TCNNGA 8 cut(s) 148, 194, 229, 291, 370, 613, 643, 695
Hpy8I GTNNAC 5 cut(s) 31, 52, 433, 493, 724
Hpy99I CGWCG 8 cut(s) 23, 26, 56, 195, 248, 281, 308, 395
HpyAV CCTTC 4 cut(s) 148, 235, 569, 692
HpyCH4III ACNGT 5 cut(s) 103, 191, 248, 346, 728
HpyCH4IV ACGT 7 cut(s) 54, 363, 435, 468, 489, 513, 525
HpyCH4V TGCA 2 cut(s) 158, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 565
HpyF3I CTNAG 2 cut(s) 414, 571
HpySE526I ACGT 7 cut(s) 54, 363, 435, 468, 489, 513, 525
Hsp92I GRCGYC 1 cut(s) 440
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 81, 90, 287, 352, 578, 688
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 4 cut(s) 92, 133, 168, 276
Lsp1109I GCAGC 3 cut(s) 307, 326, 543
MaeI CTAG 3 cut(s) 329, 503, 705
MaeII ACGT 7 cut(s) 54, 363, 435, 468, 489, 513, 525
MaeIII GTNAC 5 cut(s) 117, 169, 194, 340, 346
MalI GATC 6 cut(s) 83, 92, 289, 354, 580, 690
MboI GATC 6 cut(s) 81, 90, 287, 352, 578, 688
MboII GAAGA 6 cut(s) 26, 249, 252, 437, 634, 652
MflI RGATCY 2 cut(s) 287, 352
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 2 cut(s) 85, 380
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 5 cut(s) 222, 552, 557, 620, 702
MseI TTAA 3 cut(s) 30, 270, 754
MslI CAYNNNNRTG 2 cut(s) 534, 684
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 320, 565
NdeII GATC 6 cut(s) 81, 90, 287, 352, 578, 688
NmuCI GTSAC 4 cut(s) 169, 194, 340, 346
PaeR7I CTCGAG 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PkrI GCNGC 4 cut(s) 316, 319, 322, 558
Ple19I CGATCG 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 436
Psp124BI GAGCTC 1 cut(s) 285
Psp1406I AACGTT 1 cut(s) 489
PspCI CACGTG 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 287, 352
PvuI CGATCG 1 cut(s) 93
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 2 cut(s) 534, 684
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 3 cut(s) 30, 270, 754
SatI GCNGC 4 cut(s) 315, 318, 321, 557
Sau3AI GATC 6 cut(s) 81, 90, 287, 352, 578, 688
SduI GDGCHC 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 229
SmiMI CAYNNNNRTG 2 cut(s) 534, 684
SmlI CTYRAG 4 cut(s) 229, 402, 611, 693
SmoI CTYRAG 4 cut(s) 229, 402, 611, 693
Sse9I AATT 2 cut(s) 85, 380
SsiI CCGC 1 cut(s) 317
SspMI CTAG 3 cut(s) 329, 503, 705
SstI GAGCTC 1 cut(s) 285
TaaI ACNGT 5 cut(s) 103, 191, 248, 346, 728
TaiI ACGT 7 cut(s) 57, 366, 438, 471, 492, 516, 528
TaqI TCGA 9 cut(s) 51, 89, 93, 230, 279, 369, 462, 632, 663
TasI AATT 2 cut(s) 85, 380
TauI GCSGC 1 cut(s) 320
Tru1I TTAA 3 cut(s) 30, 270, 754
Tru9I TTAA 3 cut(s) 30, 270, 754
TseFI GTSAC 4 cut(s) 169, 194, 340, 346
TseI GCWGC 3 cut(s) 314, 320, 556
Tsp45I GTSAC 4 cut(s) 169, 194, 340, 346
TspDTI ATGAA 2 cut(s) 518, 552
TspGWI ACGGA 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 696
XapI RAATTY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 3 cut(s) 329, 503, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.